Status
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Gene information
General information
TC011868 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12387287..12389095
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC011868
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
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      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
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      Name
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      Email
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      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_02928

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection


      pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection


      larva lethality late (L7) - Number of animals showing the phenotype: 6 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011868 RA
        MNLRSKKSA TKPAKAAKN ARAKPATRP KGPEKSVNT 
        PLVPRARKK KSVNYAESS LEVTRTEAT DPVKVPIYK 
        QPLKDTPKK RKGDLYEFD SDSEKYKKK RRQKGDEDS 
        SLDEYNKTI AGILRNIKK RAQKRARKR IYTVKKTEK 
        KVQKEKVPV VLLERIPLE KLQKIARPE HETITDDFF 
        NFDVNENLD SPKINILSD VQLQPPCEL NLSLASPAL 
        FKQGRPDAN STMVNVLTP WRFNEVNIR RNPHFITLK 
        ESALPSINQ EMVLDHTIV DKFHEIENT PMRKQPESR 
        KTQKSITDY FPNNDDKEN VNTTQSSLF DNDQLSPLK 
        NRSTFERVN TVKRRILGE RSENVCANN VSNFGFTSI 
        EDEGTEEAL PGRNSDQKP FRFSVCEND KKYVRKRKV 
        RIVEKLPSS DDESEESLS DVESRSGEV RLFEDFEPT 
        QEFRELQDD KTITKKKKI DNKKHRKRK DEMTKEEEQ 
        EYEKWASAF NSMCEQVEE FPLEIE
      CDS sequence
      • >TC011868 RA
        atgaatctc agatcgaag aaaagcgcg acaaaaccg 
        gccaaagcg gcgaaaaat gctcgtgcg aagcctgcg 
        acccggccc aagggcccc gagaaaagt gttaacacc 
        ccgcttgtg cccagagcc aggaaaaag aagagtgtc 
        aactacgcc gaaagcagt ctagaagtg acccgaacc 
        gaggcaact gacccggtt aaagtgccc atttacaag 
        cagccgctc aaagacacg ccgaagaag cgcaaagga 
        gacctgtac gagtttgac tcagacagc gaaaagtac 
        aagaagaag aggagacag aagggcgat gaggactca 
        agtctggac gaatacaac aagacgatc gctggtatt 
        ttacgaaac atcaaaaaa agggcacaa aagcgggct 
        aggaaacgt atctacacc gtcaaaaaa accgaaaag 
        aaggtgcaa aaggagaag gttccagta gtactattg 
        gagcgaatc ccgctcgaa aaactacaa aaaatcgca 
        agacctgaa catgaaaca ataaccgat gattttttc 
        aatttcgac gtgaacgaa aacctcgac agccccaaa 
        ataaatatt ctcagtgac gtccagctg cagccccct 
        tgcgagttg aacttgagc cttgccagc cccgcgttg 
        ttcaagcaa gggcgacct gacgccaac agcaccatg 
        gtcaatgtt ttaacaccg tggcgtttc aacgaagtc 
        aacattcgg cgtaacccc cactttatc accctcaaa 
        gagtcggcc ctgcctagc atcaaccaa gaaatggtc 
        ctggaccac accattgtg gacaaattc cacgagatt 
        gaaaacacg ccaatgcgg aaacagcca gaaagccgc 
        aaaacgcag aaaagcatc actgattat tttcccaat 
        aacgacgac aaggaaaat gttaacacc acccagagc 
        agtctcttt gacaacgac cagctcagt ccgctgaaa 
        aacaggtcc acgtttgag agagttaac acagttaag 
        cggaggatt ttgggcgaa cggagcgaa aatgtgtgc 
        gcaaataac gtgtcaaac tttgggttt acttcgatt 
        gaagatgag ggcactgag gaggctttg ccgggcagg 
        aacagtgac cagaaaccg ttcaggttt tctgtgtgt 
        gagaacgac aagaaatac gtgcgaaag cgcaaagtt 
        agaattgtg gaaaagtta ccgtcaagt gatgacgaa 
        agtgaagag agtttaagt gatgttgag agccgaagt 
        ggtgaagtg cggctgttt gaagacttt gaacccacg 
        caggagttt cgagagtta caggacgat aaaaccatc 
        acaaagaag aagaagatt gataataag aaacatagg 
        aaacgtaaa gatgaaatg actaaagaa gaggaacaa 
        gagtatgaa aagtgggcg tccgccttt aattccatg 
        tgtgaacag gtggaggag tttccgtta gaaatcgaa 
        taa
      mRNA sequence
      • >TC011868 RA
        aaaatgaat ctcagatcg aagaaaagc gcgacaaaa 
        ccggccaaa gcggcgaaa aatgctcgt gcgaagcct 
        gcgacccgg cccaagggc cccgagaaa agtgttaac 
        accccgctt gtgcccaga gccaggaaa aagaagagt 
        gtcaactac gccgaaagc agtctagaa gtgacccga 
        accgaggca actgacccg gttaaagtg cccatttac 
        aagcagccg ctcaaagac acgccgaag aagcgcaaa 
        ggagacctg tacgagttt gactcagac agcgaaaag 
        tacaagaag aagaggaga cagaagggc gatgaggac 
        tcaagtctg gacgaatac aacaagacg atcgctggt 
        attttacga aacatcaaa aaaagggca caaaagcgg 
        gctaggaaa cgtatctac accgtcaaa aaaaccgaa 
        aagaaggtg caaaaggag aaggttcca gtagtacta 
        ttggagcga atcccgctc gaaaaacta caaaaaatc 
        gcaagacct gaacatgaa acaataacc gatgatttt 
        ttcaatttc gacgtgaac gaaaacctc gacagcccc 
        aaaataaat attctcagt gacgtccag ctgcagccc 
        ccttgcgag ttgaacttg agccttgcc agccccgcg 
        ttgttcaag caagggcga cctgacgcc aacagcacc 
        atggtcaat gttttaaca ccgtggcgt ttcaacgaa 
        gtcaacatt cggcgtaac ccccacttt atcaccctc 
        aaagagtcg gccctgcct agcatcaac caagaaatg 
        gtcctggac cacaccatt gtggacaaa ttccacgag 
        attgaaaac acgccaatg cggaaacag ccagaaagc 
        cgcaaaacg cagaaaagc atcactgat tattttccc 
        aataacgac gacaaggaa aatgttaac accacccag 
        agcagtctc tttgacaac gaccagctc agtccgctg 
        aaaaacagg tccacgttt gagagagtt aacacagtt 
        aagcggagg attttgggc gaacggagc gaaaatgtg 
        tgcgcaaat aacgtgtca aactttggg tttacttcg 
        attgaagat gagggcact gaggaggct ttgccgggc 
        aggaacagt gaccagaaa ccgttcagg ttttctgtg 
        tgtgagaac gacaagaaa tacgtgcga aagcgcaaa 
        gttagaatt gtggaaaag ttaccgtca agtgatgac 
        gaaagtgaa gagagttta agtgatgtt gagagccga 
        agtggtgaa gtgcggctg tttgaagac tttgaaccc 
        acgcaggag tttcgagag ttacaggac gataaaacc 
        atcacaaag aagaagaag attgataat aagaaacat 
        aggaaacgt aaagatgaa atgactaaa gaagaggaa 
        caagagtat gaaaagtgg gcgtccgcc tttaattcc 
        atgtgtgaa caggtggag gagtttccg ttagaaatc 
        gaataagag ctttagggc gactgagcg ttttattta 
        tttttatat tctctttgt tttaacgtg tcattgtgt 
        gactgtttt tgccacaac tactttatt tatgttatt 
        ttatgaatt attatcctt agaaactgg atttattta 
        atttacaat tacttttat ttatgggaa tgtacaaca 
        gatgcgttt tatccattt atatcgaat tcaaaacgt 
        aacaataaa aaatcaaat tgaaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY