Status
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Gene information
General information
TC034409
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011884 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12252564..12257002
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034409
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02927

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     larva segment partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034409 RA
      MCRVAIFAC VLLLVSSED VLIRTTDSS LISASTELC 
GSDAEIELT KSGETSTMT VTLSPLEHN SVCKMRVKA 
PATHVVYVQ FVEPTPEVK RALKLNATT NHPPCVMSF 
FLPENPRVP VWKGDPCSE NALTEVDLL TPDFKIIWN 
PPANPAFTK GRKFVLTAV GQGAVCKEE GQHTCMRIG 
WDPMMCVAD NLLCNGFPN CPKSSTTSD EDEKLCKKS 
FFAPTSLEQ LAVELFKKW KPPELSNKW LHEKLDTAS 
PTPPQRKPF ISWREWQDV RKHESTTAA PEQTTPKKP 
PKATDSISQ VLSKYGPWG YLMLGMLIC GTVLMFCGL 
WECCFKKPK TEIEPPVTQ TQPTTVLII NQEEASPSP 
PNYDELDQP PSYTTLFPN FKVIQNPDD SGHSTNNCQ 
VQEDSTNSC NVQDNTNCN DESNMQSVD ET
    CDS sequence
    • >TC034409 RA
      atgtgcaga gtggctatt tttgcctgc gtgctttta 
ctagtcagt tcagaagac gtcctcata cggaccacc 
gattcttcg ttaatcagc gccagtaca gagctatgc 
ggctctgat gcagaaata gaattgacc aaatcaggg 
gagacatca acaatgaca gtcacgttg tcgcccctc 
gaacacaac agcgtatgc aaaatgcgc gtcaaggcc 
cccgcgacc cacgtggtc tacgtccag ttcgtggag 
cccaccccc gaggtgaaa cgcgccctc aaactcaac 
gccaccacc aaccacccc ccgtgcgtg atgagcttc 
tttctgccc gaaaacccg cgcgtgcca gtctggaag 
ggcgatccg tgctcggaa aacgccctc acagaagtg 
gatctgctc acccccgac ttcaaaatc atctggaac 
cctcccgca aaccccgcc ttcaccaag ggaaggaag 
ttcgtcctg acggcggtc ggccaaggc gccgtgtgc 
aaggaggag ggccagcac acctgcatg cggataggc 
tgggacccc atgatgtgc gtcgcggac aacctcctc 
tgcaacggc ttccccaac tgccccaag agctccaca 
acgagcgac gaggacgag aaactctgc aagaagagt 
ttcttcgcc ccgacgtcg ctcgagcaa ctggccgtg 
gagttgttc aaaaagtgg aagccgcca gagttgagc 
aacaaatgg ctgcatgag aagctagac accgcctcg 
ccgaccccg ccccagcgc aagcccttc atcagctgg 
agggagtgg caagacgtg cggaaacac gaaagcacg 
accgcggcc ccggaacag accacgccc aagaagccg 
cccaaagcc acagactcc atctcgcag gtcttgtcc 
aagtacgga ccgtggggg tatttgatg ctggggatg 
ctgatttgt gggacggtt ttgatgttt tgtggtttg 
tgggagtgt tgtttcaag aaaccaaag actgagatt 
gaaccgccg gtgactcag actcagccc actacagtg 
ctgatcatc aaccaggag gaggcgagt ccgagtccg 
cccaactac gacgagctg gaccagccg ccttcctac 
accactctc tttccgaat tttaaagtg attcagaac 
ccggacgat agtgggcat agcacgaac aattgccaa 
gtgcaggag gacagtacg aactcttgt aacgtgcaa 
gacaatacg aactgtaac gatgaaagt aatatgcaa 
agtgttgac gaaacctaa
    mRNA sequence
    • >TC034409 RA
      actccccac gaaccggca atctagaat ttcaaagaa 
ccaatcctt aagcgctag tacaccgtt cggatgtgc 
ggccgagag tcgtgccgg tttttccgt gaaaaatgc 
ccagcgcat cagtgaaag tgtgcattt gatgcgacg 
aagaaggaa aaaattttt tgtgtacct gaggtgttc 
ctaaaaacc gaaaatgtg cagagtggc tatttttgc 
ctgcgtgct tttactagt cagttcaga agacgtcct 
catacggac caccgattc ttcgttaat cagcgccag 
tacagagct atgcggctc tgatgcaga aatagaatt 
gaccaaatc aggggagac atcaacaat gacagtcac 
gttgtcgcc cctcgaaca caacagcgt atgcaaaat 
gcgcgtcaa ggcccccgc gacccacgt ggtctacgt 
ccagttcgt ggagcccac ccccgaggt gaaacgcgc 
cctcaaact caacgccac caccaacca ccccccgtg 
cgtgatgag cttctttct gcccgaaaa cccgcgcgt 
gccagtctg gaagggcga tccgtgctc ggaaaacgc 
cctcacaga agtggatct gctcacccc cgacttcaa 
aatcatctg gaaccctcc cgcaaaccc cgccttcac 
caagggaag gaagttcgt cctgacggc ggtcggcca 
aggcgccgt gtgcaagga ggagggcca gcacacctg 
catgcggat aggctggga ccccatgat gtgcgtcgc 
ggacaacct cctctgcaa cggcttccc caactgccc 
caagagctc cacaacgag cgacgagga cgagaaact 
ctgcaagaa gagtttctt cgccccgac gtcgctcga 
gcaactggc cgtggagtt gttcaaaaa gtggaagcc 
gccagagtt gagcaacaa atggctgca tgagaagct 
agacaccgc ctcgccgac cccgcccca gcgcaagcc 
cttcatcag ctggaggga gtggcaaga cgtgcggaa 
acacgaaag cacgaccgc ggccccgga acagaccac 
gcccaagaa gccgcccaa agccacaga ctccatctc 
gcaggtctt gtccaagta cggaccgtg ggggtattt 
gatgctggg gatgctgat ttgtgggac ggttttgat 
gttttgtgg tttgtggga gtgttgttt caagaaacc 
aaagactga gattgaacc gccggtgac tcagactca 
gcccactac agtgctgat catcaacca ggaggaggc 
gagtccgag tccgcccaa ctacgacga gctggacca 
gccgccttc ctacaccac tctctttcc gaattttaa 
agtgattca gaacccgga cgatagtgg gcatagcac 
gaacaattg ccaagtgca ggaggacag tacgaactc 
ttgtaacgt gcaagacaa tacgaactg taacgatga 
aagtaatat gcaaagtgt tgacgaaac ctaacgccc 
cgcaaagac tttaggaat ctatgtatt gtgataatt 
aattctcga ctgactgag ttattttta atgttaacg 
caactgaac cctttttta ataaacctt gtaaaaagt 
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    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY