| General information | |||
| TC012551 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 11281604..11312373 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC012551 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02926 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
head appendages position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head appendages position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC012551 RA
MERLSCLLL VLFVFPHLV KLELPRYLQ AKYGMSASP SPHSVKSGV KTKTKTAAA RPPAFGSKF RGGCDSEPP KPQNAGIRC SQYSGCRAD CENLYQFPN GATQLFITC ENGQWIIEG GMWPYVPSC QPICAPPCQ NSGICVAPN QCQCPENFS GPYCEFEER PCMNYPVLP TNSRRSCSH KTCTVSCLH GHQFPDGSA IATISCKNG FWVPDKDKW KQLPDCQPT CTPPCLNGG NCLSFNRCQ CPQDFRGPQ CQYRTDNCD PRKLQFNGG YNCSGDLLS FSCSLHCPE GMNFETTPS YLYTCLYEE ATFKPQPIP QCVFPEGVS GTIRQTSSH NYYQISNNS GFNLKSLSV NGLPDVSYI PAGQRWSPY DHEMSGQLY YDSSSGLKF ATVSPYNQQ TNLIFVQEK LPEPGICLT WQGTHYKTF DGKVFSFDS KCAHTLVQD STDNTFSVV VQNDPQCYQ NPLDCFRII RIYFDKKEY ILRRSAEGP TFGTSKKRL PIPGQLPGL RIEMSAHYV IVSLDAVGA KIKWDGLQL VQVEVSESL WNRTQGLCG SIDGDVSND LLNKNGHKP QTISSFASS WKIESLEEE CNDLPSEEH ACVEDSKGH QATSFCQKL LNDQRFAPC HDVMDVNLL LDACRWDFC GCKTDPSVC ACETMNVYV RACAYKGVT NLAAWRDEN TCPMQCTGG KIYQPCAPP GGQPVCGST TEHSEEGCV EGCYCPPGT VLHDNKCIT RDKCPCRLR TKQFPPGAE VPKECNTCT CSEGKWVCT QLSCGSRCA AVGDPHYVT FDGKRYDFM GQCSYYLVK TGTFSIEAE NVACSGAIS QAMNLPTSL AEGLPSCTK TVTLRAEGQ VIKLKQNHD VVVNGRDVA KVPYQIGNV TIRSVSSIF LQVELGNGM QVWWDGVTR AYIDIPANF PEKTKGLCG TFNHNQKDD FLTPEDDIE QSVTPFANK WKTSEKCSD IPDILKTHP CSSNIHNQP TAEKHCQRL KSELFAPCH WIVDPEPYY QDCLYDMCS CEFKISKCL CPTVAAYAE ECSRQGVKI SWRDSVREC GVHCPAGQK YQVCGNSCT RTCFDVATR PDCRPQCVE GCNCPEGEA LDDIGECVP IGECQCHHD GLQFHAGYN EVRPASKGP ELCTCINAV WSCRLATPQ EQEKFPKAT DLKSLCAAS ENMEFTTCE PPEPVTCKN MHSPDYFTA SVCHPGCKC KDNYVLDTT SRKCVKPAE CPCHHGGRS YKENETVKN DCNTCKCQN GKWKCTDRP CSGECSAWG DSHFKTFDG KHYDFQGQC DYVLSKGVI GSDSYEISI QNVPCGSLG TSCSKSVTI RVKSGEDQD VLTLTRDKP LPDLAPLKH ITVHQKGLF VITEAPDLG LTIKWDKGT RVYVKIDPR WKERVKGLC GNYNDNDAD DFQTPSGGL AEASAKIFG DSWKLQSYC PEALEITNT CEDRPDRKV WALKQCGVL KSPLFAPCH SEVPVDIFL EKCVFDSCA CDQGGDCEC LCTALAAYA QECNTHGVP IKWRSQKLC PMQCDTRCS NYNPCVSTC PIETCDNLL TQVGKNCKE ETCIEGCEA KPCPPDHIY ANSSMFECV PRNVCKPIC AEIDGVTYF EGDLVEGDE CYSCFCSRG QKVCKGQPC STTPLPTTH QLEQNMKCE SGWSQWINQ DKVKPTKTG LRKKQSDYE PLPTLLLLN NLEGGKCSQ DKMVGIECR TVKTHLTPK QTGLDVECS LEKGLICKS PTKGKLCQD FEIRVLCQC EEATTTVVT PCDPVKPHK EHPTNCHIF YHCEDGPTG PKYVEKTCG PSMYYNPVT MICDWPYAV EEIKPECKQ PETVTVEVE PEAEMELPE NKTHQNLEE SQVLPVTVQ KCPPGSQEH ECAIQCDHL CLYYAHVIK TSGKCQSGE KCEPGCVRN EKNVTCPEG MFWSSEDSC VSLGDCLCR SNDGKPVKP GTVYRESDC RVCQCINNY YTCDESECE GKEEGRLLE SQLTHYNGK EVKVELATT ILPLVPSTV TPPEKCDQD HFIDLIQGD QALPDVTFN ASSVLSSAF RPEFAKFNS KVGAKSGGS WAPEYSNNL QFLEINLGQ QEPVYGVKV KGSPLYDEY VTSYKMTYS PDLSGHFFP VLNKDRLPQ VFRGSIDAN TPVQELFDV PFEAQVVRI HPETWHTGI ALRVELIGC GEPLTTTVL YEFITIHAA TSPKPMCDD PMGLDDQMM SDQQVTVSS ELDNNHTKA NLRVNGDDV WQPLTNSVT EFVQFDFLE PRNVTGVET KGGPNGWVT AYTVQYSHD NKAWNPILD KKTMKEKTF LGNFDSNTP QINNFELPI NARYVQLVP KKWNENIQM RVEMHGCFE PYPTLPEEP STVIPPACS YCPGVTTEP LELHACRCK PEKWWDGEN CVSRTECPC IIGHISYPV GTSFKKEDC SECLCKIGG VPHCTPKQC ETCEKGLRS TVTSTCKCT CQPCPDDTI LCPTSSVCI NATLWCNGV QDCPDDEVG CPTTVQTTT TPKPTTVVT QKPVVKCPE LECPTGFKR HVKRPSKGR KYQSSMFSN YQSHRKTSK SYAGVKSGL KTTYRKTPL KKPIRPQNV NNCTEYVCV SAKPAPKYM HSKKECPPA MCPEGYVPF FGNEDFVMN QCPEYTCEL APKPDAVCN VTGRTFNTF DNTEYKYDV CNHILARDL ENDEWEVTL RKNCVDKCW RDLIIQHRD HLFVLHPNL TAEYDGFSY TVEQTKKIG SNTKSFALS RLGNTILFV SNRYGFWAI WDKEGNVKL GVTRKLVDK VDGLCGYFN EDASDDKKK PDGSLARTT VDFGDSWAH VDQFQSCEP QTCPLHLQK KAWEICQVK ERVLEPCTS VIDKDSFIS RCVETTCDC LQAAGPNST ASEECRCHS LENFVVECL TRNPNLDIS DWRTLLDCP ATCDAPLVY HDCYQRKCE PTCESLSNP ELACPKLPN VCFPGCYCP SGMVKKGDT CISPSNCRD CECNVLPHL QYVTYDERN FTINANCVY VMSRDVADN HKFQVLITN APCKNNEEK TCVGKVTIL YQGHKIHVL NDMIRNKLK LIVDGEKID DFTEITWGQ IRETSSKHF KIHLVEVQV EVSVYYPSL GVSVKAPST RYGGKLEGL CGDCDHNPL DDFRTPSGA ITNDTNDFA LSWLYDKLP GGQSREMCQ NKPDDCPPL PKDQDPCNL ILDYKTFGQ CLRVLDPSL FLEWCQKDT CDNHPELAC SAIEAYARD CASAGFCID WRTDVCPKT CPSDKIYNP CGSKCPKTC QSVKEKEKG CSNLPVEGC FCPEGLVLR NDTCVEEKD CEVCDEEGH HPGDTWKKD KCTRCTCEG TSIKCETEH CSSDKICEQ GFQVVKIPG KEDECCDKY ACVPEPTSG PTCEPPQVI SCGPGQIMK LSTKPNGCQ QFICECKPV EECEPINQA LEYPLDEGF VRTIDESGC CPELKLICK TETCPKPKF CPQYHTLRN ETGKCCPWY TCEPPKNKC IYDYEYTSD ENGGERPRT AIEKQKVLK NANETWNDG PCRVCKCLR TSIGNYQAS CTQTDCSAI EISQDYLEY ELEPEFVYD QCCPNIKRV ACKHGEKVY KVGQNWTLD HDFCTVYEC VNGTSGVQK ETQVKSCDT ECELGFEYV PSTKQCCGS CKPYACVVE GEVHKIGTE WTSSDFCTK YSCLNTNGS IQVQSTRVT CPELPTDYI DNFVFETIP VAEECCKKH KTTACKVDG KVYNVGETW PSPDGDKCK KIACVRNKK GEVVKQESV ETCKTNCSK GWEYKESET SCCGECARV ACVVDERLK KAGESWTSP DNCTTYVCE QFGDEFIVS SQQESCPSL EDCPEENIY IKGCCKHCN ITSGSQQQC SAEAIPLNK TISLISAHK SDHGKCVNK KHIHKFTEC VGSCHSATF FDTKKGSHE SVCSCCHAT DYKSIEVEL ECEDGYTWK KKVAVPSAC GCEGCAAAH KSVKHKKTF GFC
- >TC012551 RA
atggaaagg ctttcgtgc cttctgttg gttcttttc gtgtttcca cacttggtt aaattagag ttgccacga tatttgcaa gcgaaatat ggcatgagc gcctctccg agtccacac tccgtgaaa agcggtgtg aagaccaag acgaaaacc gccgccgcc cgccccccg gccttcggc tcaaagttc cgcggagga tgcgattcc gaacccccc aaacctcaa aatgcagga attcgctgc tcgcaatac agcggttgt cgcgctgat tgcgaaaat ctctatcaa tttcctaac ggcgccaca cagctattt atcacttgc gaaaacggc caatggatt atcgagggc ggaatgtgg ccatacgtg ccttcctgc caaccgatt tgtgccccc ccttgccaa aacagcggc atctgtgtg gcccccaac cagtgccag tgccccgag aacttctca ggaccgtac tgcgagttc gaggagagg ccctgcatg aactaccca gtcctgccc acgaactcg cgccgctcc tgctcacac aagacgtgc acggtgtcg tgcctacac ggccaccaa ttccccgat ggttcggcc attgcaacc atttcgtgc aaaaacggc ttttgggtc ccggataag gacaagtgg aaacaatta cccgattgc caacccacc tgtacccca ccatgcctg aacgggggt aactgtctt tcgttcaac cggtgccag tgcccgcag gactttcga gggccccaa tgccaatac cggaccgat aattgtgac cctcgcaaa ctccaattt aatggaggc tacaactgc tccggcgat ctcctaagt ttcagctgc tccttacac tgtcctgaa ggaatgaat tttgagacg actccctct tacctttat acttgtttg tatgaagaa gccacgttt aaaccacaa ccaatcccg caatgcgtt tttcccgaa ggtgtttca gggacgata cgacaaacc tcctctcat aattattac caaatatcg aataattcc ggcttcaat ttaaaaagt ctttcggtg aatggttta ccggacgtt tcttatatt ccagcgggt cagagatgg tccccctat gaccacgaa atgtcggga caactctat tatgacagt tccagtggt cttaagttt gccactgtt agtccctac aaccaacaa accaactta atctttgtc caagaaaaa ttgcccgag cctggcatt tgcctgact tggcaaggc actcattac aagaccttt gacggaaaa gtcttcagt tttgactca aaatgcgcc cacactctc gtgcaggac tccacagac aatacgttc agtgtggtc gtccaaaac gacccacag tgttaccaa aaccctcta gattgtttc agaatcatt cgcatttat ttcgacaaa aaagagtac attttgcgg cgatcggcc gaaggccca actttcgga acctcgaaa aaacgcctt ccaatcccg ggccaactc ccgggattg aggatcgaa atgtccgcc cactacgta attgtgtct ttggacgcc gttggtgcg aaaataaaa tgggacggc ttgcaattg gtccaagtt gaagtgtcg gaaagcctc tggaaccgc actcagggc ttgtgcggg tccatagac ggcgatgtt tccaacgat ttgctcaat aaaaacggc cacaagcca caaacaatc tccagtttc gcctcaagc tggaaaatc gagagtttg gaggaggag tgcaatgat ctcccgtcg gaagaacac gcttgtgtg gaggactcg aaggggcac caagccacc tccttttgc cagaagttg ttgaacgac cagaggttt gccccctgt catgatgtt atggacgta aatctgctt ctggatgct tgtcggtgg gacttttgc gggtgcaaa acggaccct tcggtttgc gcttgcgag actatgaac gtctatgtg agggcttgt gcctacaag ggggtgacg aatttggcg gcgtggagg gacgaaaac acgtgtcca atgcaatgc actgggggc aaaatctac caaccctgc gcccccccc ggcggccag cccgtgtgc ggctccacc accgaacac agcgaagag ggctgtgtt gagggctgc tactgcccc cctggcacg gtcctgcac gacaacaag tgcataact cgcgacaag tgcccttgc cgcctccgc accaagcag ttccccccc ggagccgaa gtgcccaaa gagtgcaac acttgcacc tgcagcgag ggcaagtgg gtctgcacc cagctgtcc tgcggctcg cggtgtgcc gccgtcggc gacccccac tatgtcacg ttcgacggg aaacgctac gacttcatg ggccagtgt tcctactat ttggtcaaa actgggact ttttcgatc gaagcggaa aacgtcgcc tgctcaggg gccatttcg caggcgatg aacttgccg acttcgctg gccgaaggc ctgccctcg tgcaccaaa accgtgact ttgagggcc gaaggccag gtcatcaag ctcaagcaa aaccacgat gttgtggtg aacggacgg gacgtggcc aaagttccg taccagatt ggaaatgtt actataagg agcgtttcg tcgattttt ttgcaagtt gagctgggg aacggtatg caggtttgg tgggacggc gtaacgcgg gcctacatt gacattcct gccaatttc ccggaaaaa accaaagga ctctgtggt actttcaac cacaaccaa aaggacgat ttcctcaca cctgaggac gacatcgag caaagcgtt actcccttt gccaataaa tggaaaacg agcgagaaa tgtagcgac atccccgac atcctcaaa acccatcct tgcagctcc aatatccac aaccaacca acggcggaa aagcactgc caaagactc aaatccgag cttttcgcc ccttgccac tggattgtc gacccggag ccctactac caggactgt ctctacgac atgtgctcc tgtgagttc aaaatctcc aagtgcttg tgccccacg gtggcggcc tacgccgag gagtgttcc cggcaaggg gtgaaaatc tcctggagg gacagtgtg agggaatgc ggggtgcat tgcccggcg ggccagaag taccaagtt tgtgggaac tcgtgcacc aggacgtgc ttcgacgtg gccacgagg ccggactgc aggccccag tgcgtggaa gggtgtaac tgtcccgag ggcgaagcc ctggacgac atcggcgag tgtgtgccg attggggag tgccagtgc caccacgac ggcctccag ttccacgct ggatacaac gaagtcagg cctgccagc aaagggccc gagctgtgc acttgcata aacgccgtg tggagctgc cgtttggca accccccaa gaacaggaa aaatttccc aaagcaact gacctgaaa tcgctctgt gctgcctcc gaaaacatg gaattcaca acttgtgag ccgcccgaa ccggtcact tgcaaaaat atgcacagt ccggactac ttcacggcg tcggtgtgc catccaggg tgcaaatgt aaagacaac tatgtgctg gataccacg agtaggaag tgtgtcaag ccggccgaa tgtccctgc catcatgga ggaaggagt tacaaggag aatgaaacg gtcaaaaac gactgtaac acttgtaaa tgccaaaac gggaagtgg aagtgtaca gacaggccc tgttctggc gaatgcagt gcttggggg gactcacat ttcaagact tttgatggg aaacactat gattttcaa gggcagtgc gactatgtg ctgagtaag ggggttatt gggagcgat tcgtacgaa atttccatt cagaacgtt ccttgcggg tcccttggc accagctgc tccaaatcg gtcacaatc cgggtcaaa tccggcgaa gaccaagac gtcctcacc ctcactcga gacaaaccc ttacccgac ctagccccc ctaaaacac ataacagta caccagaaa ggccttttc gtcataacc gaagcccca gatttggga cttacaatc aagtgggac aaaggcaca agggtgtac gtcaaaatt gaccctcgc tggaaggaa agggtgaag ggtttgtgt ggcaactac aacgacaac gacgccgac gacttccaa acaccttcg ggaggtttg gctgaagcc tcggcgaaa attttcgga gactcgtgg aaactccag tcttactgt cccgaagct ttggaaatt acaaacact tgcgaggac cgaccggac cgaaaagtc tgggcattg aaacagtgc ggggttttg aagtcgccc cttttcgcc ccttgtcat tccgaagtc ccagtggac atatttctg gaaaagtgc gtttttgat tcatgtgcg tgcgaccaa gggggcgat tgcgagtgt ttgtgcaca gctttggcg gcctacgcc caagaatgc aacacgcac ggggtcccg attaagtgg cgatcgcaa aaactgtgt ccaatgcag tgcgacaca cgctgctcc aactacaac ccgtgtgtg tccacttgc cccattgaa acctgcgat aacctcctg acccaagtg ggcaagaac tgcaaagag gagacttgt atcgagggc tgtgaggcc aagccgtgt cctcccgac cacatctac gccaattca tcgatgttc gaatgcgtt ccgcggaac gtctgcaaa ccgatttgt gcggaaatc gacggagtg acgtatttt gagggagat ttggtcgaa ggggacgaa tgttacagt tgtttttgc tcccgaggg cagaaagtg tgcaaggga caaccctgt tcgaccaca ccattacca acaacgcac caattggaa caaaatatg aagtgtgag tctggatgg agtcagtgg atcaatcag gataaggtc aagccgacc aaaaccgga ctaaggaag aaacaaagt gattacgaa ccgctacca actctccta cttctgaat aacttggaa ggcggtaag tgcagtcag gacaaaatg gttggtatt gaatgccga actgtaaag actcatctg acgcccaaa caaaccggt cttgatgtt gaatgtagt ctggaaaag ggcctaatt tgtaaatcc ccgactaag ggaaagctg tgtcaagat ttcgaaatc cgggtcctc tgtcaatgc gaagaggcg accacgact gttgtcaca ccttgcgac ccggtcaaa ccgcacaag gaacaccca accaactgc cacattttc taccactgt gaggatggt cccactggt cccaaatac gtggaaaag acatgtggg cctagcatg tactacaac ccggtcaca atgatttgt gactggcct tacgctgtc gaagagatc aagccggag tgcaaacaa ccggaaact gtgactgtg gaggtggaa ccggaagca gaaatggag cttccggaa aataaaaca caccaaaat ctggaggaa tcacaagtt ttgccagtt actgttcaa aaatgccct cctggcagt caggaacac gaatgtgcc atacagtgt gaccatctg tgtctttac tacgctcac gtgattaaa acaagcggg aaatgccaa agtggcgaa aagtgtgaa cctggatgt gtacgaaac gagaaaaat gtaacatgc cctgagggc atgttttgg tcaagtgag gacagttgt gtgtctttg ggcgattgc ttgtgtcgc tcaaatgac gggaaaccg gtcaaacct ggaaccgtg tacagggag tcagattgt cgcgtctgc caatgtatc aacaattac tacacgtgc gacgaatcc gaatgtgaa ggaaaagag gaaggaaga ttactagaa tcgcaactt acgcattat aacgggaaa gaagttaaa gtagagttg gctacaact attctacca ctagtccca agcacagta acaccaccc gaaaaatgt gaccaggac cacttcatt gacctaatc caaggcgat caagccctt ccggacgtt actttcaac gctagtagt gtccttagc tcggcgttt aggcccgaa tttgccaaa ttcaacagc aaagtggga gcgaaaagc ggcgggagt tgggcgccg gagtattcg aacaacttg caatttttg gaaattaat ttggggcaa caggaaccg gtctatggg gttaaagtg aaaggaagc ccgctttat gacgagtac gtcacgagc tacaaaatg acgtacagt cccgacttg agtggacac ttcttcccg gtgttgaac aaagaccgg ctacctcaa gtttttagg ggttcgatt gatgccaat accccggtt caggaactg ttcgatgtt ccgtttgag gcccaagtt gtgagaatc catcccgag acgtggcac acgggcata gccctaagg gttgagctg attgggtgt ggggagccc ttgacgacc actgtgctc tatgagttt atcacgatt catgctgca acgtccccg aaaccgatg tgtgacgat cccatgggt ttggatgac cagatgatg tcggatcag caagttact gtaagctcg gaactggat aacaatcac actaaagca aacttgagg gtaaatggt gatgatgtg tggcaacct ttgacgaac tctgtgact gaatttgtt caatttgat tttttggaa ccacgaaat gtaacaggt gttgagacg aaaggaggt cctaatggg tgggttaca gcttacact gttcaatat tcacacgac aataaagct tggaacccg attttggat aagaagacg atgaaagag aaaactttt ttggggaat ttcgattcc aacactcca caaatcaat aattttgaa ctgccgatt aatgcaagg tatgtccag ttggtgccg aagaaatgg aatgagaat atacagatg agggtcgag atgcatgga tgttttgag ccttatcct accctccca gaagaacca tccaccgtc atcccccca gcctgcagc tactgccct ggcgtaacc accgaaccc ctcgaactg cacgcttgc agatgcaag cctgagaag tggtgggac ggcgaaaac tgcgtctct cgcaccgaa tgtccttgc atcatcggc cacatttcc taccccgtt gggacctcc ttcaaaaaa gaggactgc tcggagtgc ctctgcaaa atcggcggt gtgccccac tgtaccccc aaacaatgc gagacgtgc gaaaaaggc ctcagaagc accgtcacc tcgacttgc aaatgcact tgtcagccg tgccccgac gacaccatt ctgtgcccc acaagcagc gtctgcatc aacgcgact ttgtggtgc aacggcgtc caggactgt ccagatgat gaagttggg tgtcccaca accgtccaa accaccaca acgccaaaa cccaccact gtggtcacc caaaaacct gtggttaaa tgcccagag cttgagtgc cccacaggg ttcaaaaga cacgtcaaa cggccgagc aaaggccgc aagtaccag tcgtcaatg ttttcgaat tatcagtct catcgtaaa acatcgaag agttatgcc ggagtcaag agtggactc aagacaact tatcgcaaa acacctttg aagaaacca attcggcca caaaatgta aataattgt accgaatac gtgtgtgtt tcagcgaag ccggccccc aaatacatg cattcgaaa aaagagtgc ccccctgcc atgtgccca gaaggctat gtcccattt ttcggaaac gaagatttt gttatgaat caatgtccc gagtacact tgcgagcta gcgccgaaa ccagacgct gtctgtaat gttacagga cgcactttc aacactttc gacaacact gagtacaag tacgatgtt tgtaaccac attctggcc cgagatttg gaaaacgac gagtgggaa gtgactctt cgcaaaaat tgtgttgac aaatgttgg agggacctt ataatacaa catcgggac cacttgttc gtgcttcac ccgaatcta acggctgaa tatgacggt ttttcgtac actgtcgaa cagacgaaa aaaattgga tccaacacg aaatcattc gctttgtcg cggctcggt aatactata ctttttgtc tcaaataga tacggcttt tgggccatt tgggataag gaagggaac gtcaagttg ggcgttacg cgaaaattg gtggataag gtcgatggg ctttgtggt tacttcaat gaggatgcg tctgatgat aagaaaaaa cctgatggt agtttggcc cggacgacg gtcgatttt ggcgatagc tgggcacat gttgatcaa ttccaaagt tgcgagccc caaacctgc ccgcttcac ctccaaaag aaggcctgg gagatttgc caagtcaag gaacgagtg ttggagccg tgcactagt gtcatcgac aaagactcc ttcatttcg cgctgtgtg gaaaccacc tgcgactgc ctccaagcc gcaggaccc aacagcaca gcttccgag gaatgccgc tgccactcc cttgagaat ttcgtcgtt gaatgcttg accagaaac ccaaacctc gacatatcc gattggaga acacttctc gattgtccg gcgacttgt gacgcccct ctggtttac cacgactgt taccaacgc aaatgcgaa ccgacgtgc gaatcttta tcaaatccc gaattagcg tgcccgaaa ctccccaat gtgtgcttc cctggctgt tattgcccc tcagggatg gtcaagaaa ggggacact tgtataagt ccctccaac tgtagggac tgcgaatgc aacgtcctc ccgcacctc caatatgtg acttatgac gaaaggaac ttcacgatc aatgcgaat tgtgtctat gtgatgtcg cgcgatgtg gctgacaat cacaagttt caagttttg atcaccaat gccccgtgc aagaataac gaggaaaag acgtgtgtt ggcaaagtc acgattttg taccaaggg cacaaaatc cacgtcctc aacgatatg atacgaaat aaactgaaa ctgatcgtt gatggggaa aaaattgac gatttcacg gaaatcacg tggggccaa atcagggag acttcaagt aaacacttc aagattcat ttggtcgaa gtccaagtt gaagtatcg gtctattac ccctctttg ggggtttcg gtaaaagcg ccctcgacc agatacggg ggaaagctc gaagggctg tgtggtgat tgtgaccac aaccccctt gatgatttc cggacccct tccggggct ataactaac gacacaaac gactttgcc ttgagctgg ctctacgat aaactgcca ggagggcaa tcaagagaa atgtgtcag aacaaaccg gacgattgt ccaccattg cccaaagac caggaccct tgtaatctc atcctggac tacaagacc ttcgggcaa tgtttacgt gtcttggac ccaagtctt ttccttgaa tggtgccag aaagacacg tgtgacaac cacccagaa ctcgcttgc agtgccatc gaagcctac gctcgtgac tgtgcaagt gctggcttt tgtatcgac tggagaaca gatgtttgc cccaaaacc tgtccttcc gacaagatt tataatcct tgtgggagc aaatgcccc aaaacgtgc caaagtgtg aaggagaag gagaaaggg tgttcaaat ctgccggtt gaagggtgt ttctgtccg gaaggattg gtcttgcgg aacgacact tgcgtggag gaaaaggac tgcgaggtg tgtgacgag gaagggcac catcctggt gacacttgg aagaaggat aagtgtaca aggtgcacc tgcgaaggc acttccatc aagtgtgag acggagcat tgctccagc gacaagatt tgtgagcaa gggttccaa gttgtgaaa atcccgggc aaagaggac gaatgttgc gataaatac gcatgtgta cctgagccc acttcgggc cccacttgt gagcccccg caagtaatt tcctgcggc ccgggccaa ataatgaaa ctctcgacg aaaccaaac ggttgtcag caattcatc tgcgaatgc aaaccagtc gaagagtgc gaaccaata aaccaagcg cttgagtac ccactcgac gaaggcttc gtccgaaca atcgacgag agtggctgt tgccccgaa ctaaaatta atctgcaag acggagact tgtcccaaa ccgaaattt tgcccacaa tatcacact ctcaggaac gaaactggc aagtgttgc ccttggtac acttgcgaa cccccgaag aacaaatgc atctacgat tacgaatac acttcggac gaaaacggg ggcgaacgg ccccgtacg gcaattgag aagcaaaaa gtcctgaaa aatgcgaac gagacgtgg aacgacggt ccctgccgt gtttgcaag tgtttgcgg acgagcatt ggcaattac caggcgagt tgcacccaa actgactgc tcggcgatt gaaatatcg caggattat ctcgagtat gagctagaa ccggagttt gtctacgac cagtgttgc ccgaacatc aaacgagtg gcgtgcaaa cacggcgaa aaagtgtac aaagtgggg cagaactgg acccttgac cacgatttt tgcacggtt tatgagtgc gttaatggc acttcaggg gtgcagaag gaaacgcaa gtcaagagt tgtgacact gagtgtgag ctgggcttc gagtacgtt ccctcgacg aagcaatgt tgcggttct tgcaagccg tacgcctgc gtggttgag ggggaagtc cacaaaatt gggaccgag tggacttcc agcgacttc tgtacgaaa tattcgtgc ctcaacacc aacggctcg attcaagta caatcaact agagttaca tgtccggag ttgccaacc gattatatt gacaatttt gtttttgaa acaatcccg gtagccgag gaatgttgc aagaaacac aaaaccacg gcgtgcaaa gtcgatggg aaagtctac aatgtggga gaaacctgg ccatcgcct gatggagac aagtgcaag aaaatcgca tgtgttagg aacaagaaa ggggaggtt gtgaagcag gagtcggtg gagacgtgc aaaaccaac tgttctaaa ggctgggaa tacaaagag tctgagact tcctgctgc ggcgaatgc gcgcgtgtc gcctgtgtg gtcgacgag aggttgaaa aaagcgggc gagagttgg acttccccc gataattgt acgacctac gtttgcgag caattcggc gacgaattt atagtttca agtcagcaa gaatcgtgt ccgtcgctt gaagactgt cccgaggaa aacatttac attaagggc tgttgtaaa cattgtaac attacatca gggtcgcag cagcaatgc agtgctgag gcgattccg ctcaacaaa accatttcg ctgataagc gcgcacaag tccgaccac ggcaaatgc gttaacaag aaacacatt cacaagttc actgaatgt gtggggagt tgccactcg gccacgttt ttcgataca aagaaaggt tcacacgaa agtgtgtgc tcgtgttgt cacgcgacc gattataag tcaattgaa gtcgaactg gaatgtgag gacgggtac acttggaag aagaaagtg gcagtgccg tcagcgtgt ggttgtgag gggtgtgcc gcagcacac aagtccgta aaacataag aaaactttc gggttttgt taa
- >TC012551 RA
ctagcttac ctaatcgca attttgata acttgcaaa attagctca ctataaatt cattcggtg atgaaagtt tgaagttag tgcttagtg ggttttaaa atggaaagg ctttcgtgc cttctgttg gttcttttc gtgtttcca cacttggtt aaattagag ttgccacga tatttgcaa gcgaaatat ggcatgagc gcctctccg agtccacac tccgtgaaa agcggtgtg aagaccaag acgaaaacc gccgccgcc cgccccccg gccttcggc tcaaagttc cgcggagga tgcgattcc gaacccccc aaacctcaa aatgcagga attcgctgc tcgcaatac agcggttgt cgcgctgat tgcgaaaat ctctatcaa tttcctaac ggcgccaca cagctattt atcacttgc gaaaacggc caatggatt atcgagggc ggaatgtgg ccatacgtg ccttcctgc caaccgatt tgtgccccc ccttgccaa aacagcggc atctgtgtg gcccccaac cagtgccag tgccccgag aacttctca ggaccgtac tgcgagttc gaggagagg ccctgcatg aactaccca gtcctgccc acgaactcg cgccgctcc tgctcacac aagacgtgc acggtgtcg tgcctacac ggccaccaa ttccccgat ggttcggcc attgcaacc atttcgtgc aaaaacggc ttttgggtc ccggataag gacaagtgg aaacaatta cccgattgc caacccacc tgtacccca ccatgcctg aacgggggt aactgtctt tcgttcaac cggtgccag tgcccgcag gactttcga gggccccaa tgccaatac cggaccgat aattgtgac cctcgcaaa ctccaattt aatggaggc tacaactgc tccggcgat ctcctaagt ttcagctgc tccttacac tgtcctgaa ggaatgaat tttgagacg actccctct tacctttat acttgtttg tatgaagaa gccacgttt aaaccacaa ccaatcccg caatgcgtt tttcccgaa ggtgtttca gggacgata cgacaaacc tcctctcat aattattac caaatatcg aataattcc ggcttcaat ttaaaaagt ctttcggtg aatggttta ccggacgtt tcttatatt ccagcgggt cagagatgg tccccctat gaccacgaa atgtcggga caactctat tatgacagt tccagtggt cttaagttt gccactgtt agtccctac aaccaacaa accaactta atctttgtc caagaaaaa ttgcccgag cctggcatt tgcctgact tggcaaggc actcattac aagaccttt gacggaaaa gtcttcagt tttgactca aaatgcgcc cacactctc gtgcaggac tccacagac aatacgttc agtgtggtc gtccaaaac gacccacag tgttaccaa aaccctcta gattgtttc agaatcatt cgcatttat ttcgacaaa aaagagtac attttgcgg cgatcggcc gaaggccca actttcgga acctcgaaa aaacgcctt ccaatcccg ggccaactc ccgggattg aggatcgaa atgtccgcc cactacgta attgtgtct ttggacgcc gttggtgcg aaaataaaa tgggacggc ttgcaattg gtccaagtt gaagtgtcg gaaagcctc tggaaccgc actcagggc ttgtgcggg tccatagac ggcgatgtt tccaacgat ttgctcaat aaaaacggc cacaagcca caaacaatc tccagtttc gcctcaagc tggaaaatc gagagtttg gaggaggag tgcaatgat ctcccgtcg gaagaacac gcttgtgtg gaggactcg aaggggcac caagccacc tccttttgc cagaagttg ttgaacgac cagaggttt gccccctgt catgatgtt atggacgta aatctgctt ctggatgct tgtcggtgg gacttttgc gggtgcaaa acggaccct tcggtttgc gcttgcgag actatgaac gtctatgtg agggcttgt gcctacaag ggggtgacg aatttggcg gcgtggagg gacgaaaac acgtgtcca atgcaatgc actgggggc aaaatctac caaccctgc gcccccccc ggcggccag cccgtgtgc ggctccacc accgaacac agcgaagag ggctgtgtt gagggctgc tactgcccc cctggcacg gtcctgcac gacaacaag tgcataact cgcgacaag tgcccttgc cgcctccgc accaagcag ttccccccc ggagccgaa gtgcccaaa gagtgcaac acttgcacc tgcagcgag ggcaagtgg gtctgcacc cagctgtcc tgcggctcg cggtgtgcc gccgtcggc gacccccac tatgtcacg ttcgacggg aaacgctac gacttcatg ggccagtgt tcctactat ttggtcaaa actgggact ttttcgatc gaagcggaa aacgtcgcc tgctcaggg gccatttcg caggcgatg aacttgccg acttcgctg gccgaaggc ctgccctcg tgcaccaaa accgtgact ttgagggcc gaaggccag gtcatcaag ctcaagcaa aaccacgat gttgtggtg aacggacgg gacgtggcc aaagttccg taccagatt ggaaatgtt actataagg agcgtttcg tcgattttt ttgcaagtt gagctgggg aacggtatg caggtttgg tgggacggc gtaacgcgg gcctacatt gacattcct gccaatttc ccggaaaaa accaaagga ctctgtggt actttcaac cacaaccaa aaggacgat ttcctcaca cctgaggac gacatcgag caaagcgtt actcccttt gccaataaa tggaaaacg agcgagaaa tgtagcgac atccccgac atcctcaaa acccatcct tgcagctcc aatatccac aaccaacca acggcggaa aagcactgc caaagactc aaatccgag cttttcgcc ccttgccac tggattgtc gacccggag ccctactac caggactgt ctctacgac atgtgctcc tgtgagttc aaaatctcc aagtgcttg tgccccacg gtggcggcc tacgccgag gagtgttcc cggcaaggg gtgaaaatc tcctggagg gacagtgtg agggaatgc ggggtgcat tgcccggcg ggccagaag taccaagtt tgtgggaac tcgtgcacc aggacgtgc ttcgacgtg gccacgagg ccggactgc aggccccag tgcgtggaa gggtgtaac tgtcccgag ggcgaagcc ctggacgac atcggcgag tgtgtgccg attggggag tgccagtgc caccacgac ggcctccag ttccacgct ggatacaac gaagtcagg cctgccagc aaagggccc gagctgtgc acttgcata aacgccgtg tggagctgc cgtttggca accccccaa gaacaggaa aaatttccc aaagcaact gacctgaaa tcgctctgt gctgcctcc gaaaacatg gaattcaca acttgtgag ccgcccgaa ccggtcact tgcaaaaat atgcacagt ccggactac ttcacggcg tcggtgtgc catccaggg tgcaaatgt aaagacaac tatgtgctg gataccacg agtaggaag tgtgtcaag ccggccgaa tgtccctgc catcatgga ggaaggagt tacaaggag aatgaaacg gtcaaaaac gactgtaac acttgtaaa tgccaaaac gggaagtgg aagtgtaca gacaggccc tgttctggc gaatgcagt gcttggggg gactcacat ttcaagact tttgatggg aaacactat gattttcaa gggcagtgc gactatgtg ctgagtaag ggggttatt gggagcgat tcgtacgaa atttccatt cagaacgtt ccttgcggg tcccttggc accagctgc tccaaatcg gtcacaatc cgggtcaaa tccggcgaa gaccaagac gtcctcacc ctcactcga gacaaaccc ttacccgac ctagccccc ctaaaacac ataacagta caccagaaa ggccttttc gtcataacc gaagcccca gatttggga cttacaatc aagtgggac aaaggcaca agggtgtac gtcaaaatt gaccctcgc tggaaggaa agggtgaag ggtttgtgt ggcaactac aacgacaac gacgccgac gacttccaa acaccttcg ggaggtttg gctgaagcc tcggcgaaa attttcgga gactcgtgg aaactccag tcttactgt cccgaagct ttggaaatt acaaacact tgcgaggac cgaccggac cgaaaagtc tgggcattg aaacagtgc ggggttttg aagtcgccc cttttcgcc ccttgtcat tccgaagtc ccagtggac atatttctg gaaaagtgc gtttttgat tcatgtgcg tgcgaccaa gggggcgat tgcgagtgt ttgtgcaca gctttggcg gcctacgcc caagaatgc aacacgcac ggggtcccg attaagtgg cgatcgcaa aaactgtgt ccaatgcag tgcgacaca cgctgctcc aactacaac ccgtgtgtg tccacttgc cccattgaa acctgcgat aacctcctg acccaagtg ggcaagaac tgcaaagag gagacttgt atcgagggc tgtgaggcc aagccgtgt cctcccgac cacatctac gccaattca tcgatgttc gaatgcgtt ccgcggaac gtctgcaaa ccgatttgt gcggaaatc gacggagtg acgtatttt gagggagat ttggtcgaa ggggacgaa tgttacagt tgtttttgc tcccgaggg cagaaagtg tgcaaggga caaccctgt tcgaccaca ccattacca acaacgcac caattggaa caaaatatg aagtgtgag tctggatgg agtcagtgg atcaatcag gataaggtc aagccgacc aaaaccgga ctaaggaag aaacaaagt gattacgaa ccgctacca actctccta cttctgaat aacttggaa ggcggtaag tgcagtcag gacaaaatg gttggtatt gaatgccga actgtaaag actcatctg acgcccaaa caaaccggt cttgatgtt gaatgtagt ctggaaaag ggcctaatt tgtaaatcc ccgactaag ggaaagctg tgtcaagat ttcgaaatc cgggtcctc tgtcaatgc gaagaggcg accacgact gttgtcaca ccttgcgac ccggtcaaa ccgcacaag gaacaccca accaactgc cacattttc taccactgt gaggatggt cccactggt cccaaatac gtggaaaag acatgtggg cctagcatg tactacaac ccggtcaca atgatttgt gactggcct tacgctgtc gaagagatc aagccggag tgcaaacaa ccggaaact gtgactgtg gaggtggaa ccggaagca gaaatggag cttccggaa aataaaaca caccaaaat ctggaggaa tcacaagtt ttgccagtt actgttcaa aaatgccct cctggcagt caggaacac gaatgtgcc atacagtgt gaccatctg tgtctttac tacgctcac gtgattaaa acaagcggg aaatgccaa agtggcgaa aagtgtgaa cctggatgt gtacgaaac gagaaaaat gtaacatgc cctgagggc atgttttgg tcaagtgag gacagttgt gtgtctttg ggcgattgc ttgtgtcgc tcaaatgac gggaaaccg gtcaaacct ggaaccgtg tacagggag tcagattgt cgcgtctgc caatgtatc aacaattac tacacgtgc gacgaatcc gaatgtgaa ggaaaagag gaaggaaga ttactagaa tcgcaactt acgcattat aacgggaaa gaagttaaa gtagagttg gctacaact attctacca ctagtccca agcacagta acaccaccc gaaaaatgt gaccaggac cacttcatt gacctaatc caaggcgat caagccctt ccggacgtt actttcaac gctagtagt gtccttagc tcggcgttt aggcccgaa tttgccaaa ttcaacagc aaagtggga gcgaaaagc ggcgggagt tgggcgccg gagtattcg aacaacttg caatttttg gaaattaat ttggggcaa caggaaccg gtctatggg gttaaagtg aaaggaagc ccgctttat gacgagtac gtcacgagc tacaaaatg acgtacagt cccgacttg agtggacac ttcttcccg gtgttgaac aaagaccgg ctacctcaa gtttttagg ggttcgatt gatgccaat accccggtt caggaactg ttcgatgtt ccgtttgag gcccaagtt gtgagaatc catcccgag acgtggcac acgggcata gccctaagg gttgagctg attgggtgt ggggagccc ttgacgacc actgtgctc tatgagttt atcacgatt catgctgca acgtccccg aaaccgatg tgtgacgat cccatgggt ttggatgac cagatgatg tcggatcag caagttact gtaagctcg gaactggat aacaatcac actaaagca aacttgagg gtaaatggt gatgatgtg tggcaacct ttgacgaac tctgtgact gaatttgtt caatttgat tttttggaa ccacgaaat gtaacaggt gttgagacg aaaggaggt cctaatggg tgggttaca gcttacact gttcaatat tcacacgac aataaagct tggaacccg attttggat aagaagacg atgaaagag aaaactttt ttggggaat ttcgattcc aacactcca caaatcaat aattttgaa ctgccgatt aatgcaagg tatgtccag ttggtgccg aagaaatgg aatgagaat atacagatg agggtcgag atgcatgga tgttttgag ccttatcct accctccca gaagaacca tccaccgtc atcccccca gcctgcagc tactgccct ggcgtaacc accgaaccc ctcgaactg cacgcttgc agatgcaag cctgagaag tggtgggac ggcgaaaac tgcgtctct cgcaccgaa tgtccttgc atcatcggc cacatttcc taccccgtt gggacctcc ttcaaaaaa gaggactgc tcggagtgc ctctgcaaa atcggcggt gtgccccac tgtaccccc aaacaatgc gagacgtgc gaaaaaggc ctcagaagc accgtcacc tcgacttgc aaatgcact tgtcagccg tgccccgac gacaccatt ctgtgcccc acaagcagc gtctgcatc aacgcgact ttgtggtgc aacggcgtc caggactgt ccagatgat gaagttggg tgtcccaca accgtccaa accaccaca acgccaaaa cccaccact gtggtcacc caaaaacct gtggttaaa tgcccagag cttgagtgc cccacaggg ttcaaaaga cacgtcaaa cggccgagc aaaggccgc aagtaccag tcgtcaatg ttttcgaat tatcagtct catcgtaaa acatcgaag agttatgcc ggagtcaag agtggactc aagacaact tatcgcaaa acacctttg aagaaacca attcggcca caaaatgta aataattgt accgaatac gtgtgtgtt tcagcgaag ccggccccc aaatacatg cattcgaaa aaagagtgc ccccctgcc atgtgccca gaaggctat gtcccattt ttcggaaac gaagatttt gttatgaat caatgtccc gagtacact tgcgagcta gcgccgaaa ccagacgct gtctgtaat gttacagga cgcactttc aacactttc gacaacact gagtacaag tacgatgtt tgtaaccac attctggcc cgagatttg gaaaacgac gagtgggaa gtgactctt cgcaaaaat tgtgttgac aaatgttgg agggacctt ataatacaa catcgggac cacttgttc gtgcttcac ccgaatcta acggctgaa tatgacggt ttttcgtac actgtcgaa cagacgaaa aaaattgga tccaacacg aaatcattc gctttgtcg cggctcggt aatactata ctttttgtc tcaaataga tacggcttt tgggccatt tgggataag gaagggaac gtcaagttg ggcgttacg cgaaaattg gtggataag gtcgatggg ctttgtggt tacttcaat gaggatgcg tctgatgat aagaaaaaa cctgatggt agtttggcc cggacgacg gtcgatttt ggcgatagc tgggcacat gttgatcaa ttccaaagt tgcgagccc caaacctgc ccgcttcac ctccaaaag aaggcctgg gagatttgc caagtcaag gaacgagtg ttggagccg tgcactagt gtcatcgac aaagactcc ttcatttcg cgctgtgtg gaaaccacc tgcgactgc ctccaagcc gcaggaccc aacagcaca gcttccgag gaatgccgc tgccactcc cttgagaat ttcgtcgtt gaatgcttg accagaaac ccaaacctc gacatatcc gattggaga acacttctc gattgtccg gcgacttgt gacgcccct ctggtttac cacgactgt taccaacgc aaatgcgaa ccgacgtgc gaatcttta tcaaatccc gaattagcg tgcccgaaa ctccccaat gtgtgcttc cctggctgt tattgcccc tcagggatg gtcaagaaa ggggacact tgtataagt ccctccaac tgtagggac tgcgaatgc aacgtcctc ccgcacctc caatatgtg acttatgac gaaaggaac ttcacgatc aatgcgaat tgtgtctat gtgatgtcg cgcgatgtg gctgacaat cacaagttt caagttttg atcaccaat gccccgtgc aagaataac gaggaaaag acgtgtgtt ggcaaagtc acgattttg taccaaggg cacaaaatc cacgtcctc aacgatatg atacgaaat aaactgaaa ctgatcgtt gatggggaa aaaattgac gatttcacg gaaatcacg tggggccaa atcagggag acttcaagt aaacacttc aagattcat ttggtcgaa gtccaagtt gaagtatcg gtctattac ccctctttg ggggtttcg gtaaaagcg ccctcgacc agatacggg ggaaagctc gaagggctg tgtggtgat tgtgaccac aaccccctt gatgatttc cggacccct tccggggct ataactaac gacacaaac gactttgcc ttgagctgg ctctacgat aaactgcca ggagggcaa tcaagagaa atgtgtcag aacaaaccg gacgattgt ccaccattg cccaaagac caggaccct tgtaatctc atcctggac tacaagacc ttcgggcaa tgtttacgt gtcttggac ccaagtctt ttccttgaa tggtgccag aaagacacg tgtgacaac cacccagaa ctcgcttgc agtgccatc gaagcctac gctcgtgac tgtgcaagt gctggcttt tgtatcgac tggagaaca gatgtttgc cccaaaacc tgtccttcc gacaagatt tataatcct tgtgggagc aaatgcccc aaaacgtgc caaagtgtg aaggagaag gagaaaggg tgttcaaat ctgccggtt gaagggtgt ttctgtccg gaaggattg gtcttgcgg aacgacact tgcgtggag gaaaaggac tgcgaggtg tgtgacgag gaagggcac catcctggt gacacttgg aagaaggat aagtgtaca aggtgcacc tgcgaaggc acttccatc aagtgtgag acggagcat tgctccagc gacaagatt tgtgagcaa gggttccaa gttgtgaaa atcccgggc aaagaggac gaatgttgc gataaatac gcatgtgta cctgagccc acttcgggc cccacttgt gagcccccg caagtaatt tcctgcggc ccgggccaa ataatgaaa ctctcgacg aaaccaaac ggttgtcag caattcatc tgcgaatgc aaaccagtc gaagagtgc gaaccaata aaccaagcg cttgagtac ccactcgac gaaggcttc gtccgaaca atcgacgag agtggctgt tgccccgaa ctaaaatta atctgcaag acggagact tgtcccaaa ccgaaattt tgcccacaa tatcacact ctcaggaac gaaactggc aagtgttgc ccttggtac acttgcgaa cccccgaag aacaaatgc atctacgat tacgaatac acttcggac gaaaacggg ggcgaacgg ccccgtacg gcaattgag aagcaaaaa gtcctgaaa aatgcgaac gagacgtgg aacgacggt ccctgccgt gtttgcaag tgtttgcgg acgagcatt ggcaattac caggcgagt tgcacccaa actgactgc tcggcgatt gaaatatcg caggattat ctcgagtat gagctagaa ccggagttt gtctacgac cagtgttgc ccgaacatc aaacgagtg gcgtgcaaa cacggcgaa aaagtgtac aaagtgggg cagaactgg acccttgac cacgatttt tgcacggtt tatgagtgc gttaatggc acttcaggg gtgcagaag gaaacgcaa gtcaagagt tgtgacact gagtgtgag ctgggcttc gagtacgtt ccctcgacg aagcaatgt tgcggttct tgcaagccg tacgcctgc gtggttgag ggggaagtc cacaaaatt gggaccgag tggacttcc agcgacttc tgtacgaaa tattcgtgc ctcaacacc aacggctcg attcaagta caatcaact agagttaca tgtccggag ttgccaacc gattatatt gacaatttt gtttttgaa acaatcccg gtagccgag gaatgttgc aagaaacac aaaaccacg gcgtgcaaa gtcgatggg aaagtctac aatgtggga gaaacctgg ccatcgcct gatggagac aagtgcaag aaaatcgca tgtgttagg aacaagaaa ggggaggtt gtgaagcag gagtcggtg gagacgtgc aaaaccaac tgttctaaa ggctgggaa tacaaagag tctgagact tcctgctgc ggcgaatgc gcgcgtgtc gcctgtgtg gtcgacgag aggttgaaa aaagcgggc gagagttgg acttccccc gataattgt acgacctac gtttgcgag caattcggc gacgaattt atagtttca agtcagcaa gaatcgtgt ccgtcgctt gaagactgt cccgaggaa aacatttac attaagggc tgttgtaaa cattgtaac attacatca gggtcgcag cagcaatgc agtgctgag gcgattccg ctcaacaaa accatttcg ctgataagc gcgcacaag tccgaccac ggcaaatgc gttaacaag aaacacatt cacaagttc actgaatgt gtggggagt tgccactcg gccacgttt ttcgataca aagaaaggt tcacacgaa agtgtgtgc tcgtgttgt cacgcgacc gattataag tcaattgaa gtcgaactg gaatgtgag gacgggtac acttggaag aagaaagtg gcagtgccg tcagcgtgt ggttgtgag gggtgtgcc gcagcacac aagtccgta aaacataag aaaactttc gggttttgt taaatcgta attgagatg atttatttt tgtacgttt tttcatttc tctaataaa atgcaatga agagt
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