Status
Please wait ...
Gene information
General information
TC011996 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 1261786..1275396
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011996
    iBeetle screen iB_02924
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Pupal morphology
    head not bent - Number of animals showing the phenotype: 3

    wings shortened - Number of animals showing the phenotype: 10

    wings not closed - Number of animals showing the phenotype: 10

    Adult morphology
    wings not closed - Number of animals showing the phenotype: 5


    wings slightlyshortened - Number of animals showing the phenotype: 3


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011996 RA
      MRENNLQMP PFPREYSPD FWSFDPEDS VELTCLMPN 
      GIVILLKVS YNATLLDIK ADLWEEAPK YPLHGKLHD 
      MSSYVFTCI NSMAEREEL HDETRRICD VKPTCAVLK 
      IECLGNEPD NKINVQIGH LIGRSVKEF DELNNPEIN 
      DFRFQMRKL GDDITQKRL KYTWLEKLY YQFPPRLCP 
      KSKIFPETL DQKKNIKIT TRFEDNENS FTFNVSPYI 
      YPNKLLEMI LAKRSNVLK IRYDRCNDY VLKVCGQDE 
      YLVGDHQLI QFQYIQDSL STDVTPIVV TVRVNKVPV 
      TPDNPYESL DNFDHRRMR STYSTLTWR KRAHKSAWD 
      IQRKFSVTL NAVSGLNCD MRKTNEIGI QVGLFHGGK 
      SLCERLKAS IQPINEKNE SFFRETLVF DILVCNIPR 
      NAKLCFCIY EVPKSSKLV RTKKGKEYG NNKESITPI 
      AWANTTVYD FKNQLRNGA MTLYMWTFA NDLMCEDIF 
      NALGNVVNN PNKSATALT LHLIDQTIY YPTTEAMIN 
      FAQENKSKY ISNSIEYNP TNIEHCQKI LTENDNLFE 
      VHEQDRKNI WLLKDYWKK QNPEVLPKL LSSIDWDTR 
      EVVSDVAAL LKDWPKLPP EKALELLDY AYPDQRVRS 
      FAVECLDDV SDEDLLLYL LQLVQAIKH EMYLECDLV 
      KFLIKRALN NQKIGHYLF WHLKSELQV PSVSVRFGL 
      ILEAYCRGS QEHMPVLLK QLEFLDKLK NSREHVKNK 
      SKEKAKSNL KDYLTECHI DETVQNIRS PLDPSFRCS 
      KIKLDRCRI MDSKMRPLW IVLQNYDPF GDDVYIIFK 
      DGDDLRQDM LTLQMLKIM DRLWKSEGL DLRLSPYNC 
      ISLGDRAGM IEVVLNAET IANIQKDKG VFSATSAFR 
      KGSILAWLR DHNPTESAL NKAINEFTL SCAGYCVAT 
      YVLGVADRH SDNIMVKKS GQLFHIDFG HILGHFKEK 
      FGIKRERVP FVLTHDFEY VINKGQKTT LEFKIFQEY 
      CEKAFVILR KHGSLILSL CAMMISTGL PELSSEKDL 
      NYIRDTLAL SKSEEEALN HFRNKFDEA LSNSWKTSL 
      NWASHNLAK NNKA
    CDS sequence
    • >TC011996 RA
      atgcgagaa aacaatctc caaatgcct ccatttccc 
      cgagaatac tccccggac ttctggtcc tttgaccca 
      gaagattcc gtggaattg acttgtctc atgcctaac 
      gggattgtg attctcctc aaagtatcc tacaatgcc 
      accctactg gacatcaaa gcggacttg tgggaggag 
      gcccccaag tacccgctc catggcaag ctccacgac 
      atgtcctcg tatgtcttc acctgcatt aatagcatg 
      gcagagcgg gaggagctg catgacgag acgaggaga 
      atctgcgat gttaaacct acttgcgct gtactgaaa 
      attgagtgc cttgggaat gaaccagat aacaagatc 
      aatgtccag atcgggcat ttgataggg aggagtgtt 
      aaagaattt gacgagttg aataatccc gagattaac 
      gatttccgt tttcaaatg cggaaattg ggcgatgat 
      attacgcag aaacgcttg aaatatacg tggcttgaa 
      aagttgtat tatcagttt ccgccgcga ctgtgtccc 
      aaaagcaaa atctttccg gaaacgctt gaccaaaaa 
      aagaatatt aaaattact acacgattt gaagataat 
      gagaattct tttacgttc aatgtctct ccttacatt 
      tacccaaac aaattattg gaaatgatt ctcgcgaaa 
      aggtccaac gttttgaaa atcagatac gaccgttgt 
      aacgattat gtattaaaa gtgtgtggc caggacgag 
      tatttagta ggagatcac caactcatt caattccaa 
      tacatccaa gactcttta tctacagac gtaactcct 
      attgtggtc acagtgaga gttaacaaa gtcccagtc 
      actcctgat aatccctac gaatcgttg gataatttc 
      gaccacagg agaatgcgg tcgacttat tcaacatta 
      acatggcgt aaaagagcc cacaagtcg gcttgggat 
      attcagagg aagtttagt gtgactttg aacgcagtc 
      agcggattg aattgtgat atgaggaaa acaaatgag 
      attggcatt caggtggga ttgtttcat gggggaaag 
      tcgctttgc gaaagactt aaagcttcc atccaacca 
      ataaacgaa aagaatgaa agttttttt cgtgagact 
      ttagttttt gatatctta gtgtgcaat atacccaga 
      aatgcgaaa ttgtgtttt tgcatttat gaagttcct 
      aaatctagt aagttagtt agaacgaaa aaaggcaaa 
      gaatatgga aataataag gagtctatt actcctata 
      gcttgggct aatacgacc gtttacgat tttaaaaac 
      cagctgaga aatggcgca atgacactt tacatgtgg 
      acttttgcc aacgatttg atgtgtgag gatatattt 
      aatgcttta gggaatgtt gttaataat ccgaataaa 
      agtgcaacg gccttaaca ctacatctt atagatcaa 
      acgatttat tatccaacg acggaagca atgattaat 
      tttgctcag gaaaacaaa agcaaatat attagtaac 
      agtattgaa tacaatccg acgaacatc gaacattgt 
      cagaaaatt cttaccgaa aatgacaat ttgtttgag 
      gttcatgaa caggatcga aaaaatatc tggctgttg 
      aaagactat tggaaaaaa caaaatccg gaagtcctt 
      cctaagctg ttatcttcg attgactgg gatactcgt 
      gaagtggtg agcgatgta gctgcacta ttaaaagac 
      tggcctaag cttccgcct gagaaagca ttagaatta 
      ttagattat gcatatcct gatcagaga gttcggagt 
      tttgcagtt gaatgtctg gacgatgtc agtgacgag 
      gacttactt ctttatctc ttacaatta gtacaagca 
      atcaaacac gaaatgtat ctagaatgt gatttagta 
      aaatttcta ataaaacga gcgctaaat aatcaaaag 
      atcggtcac tacttattc tggcatctc aaatccgag 
      ctgcaagtc ccatctgta tcggtccgg ttcggactt 
      attctggaa gcctattgc agagggagt caggaacac 
      atgcctgtg ttgttaaaa cagttagaa tttctcgat 
      aagttgaag aacagtcga gaacacgtc aagaacaaa 
      agcaaggaa aaggctaaa tcaaatctc aaagattac 
      ttaacagag tgccacatt gacgagacc gtgcaaaac 
      attaggagc ccgctggac cccagtttc cggtgctct 
      aaaataaaa ttagacaga tgtaggatt atggatagc 
      aaaatgcgg ccgttgtgg atcgttctc cagaattac 
      gatccgttc ggagacgat gtttacatt attttcaaa 
      gacggcgac gacttgaga caagatatg ctcacgttg 
      cagatgttg aagattatg gatagattg tggaagagc 
      gaagggttg gatttaaga ttgagtccg tacaattgc 
      atatcgtta ggagatagg gccggtatg attgaagta 
      gtgctcaac gcggagacc attgccaac atccagaag 
      gacaagggc gtgttttcg gccacttcg gccttccgc 
      aagggctcg attttggct tggttgaga gaccacaac 
      ccgacggag tcagctctc aacaaggcc atcaacgag 
      ttcaccctt tcgtgtgcc ggctattgc gtcgcgacg 
      tacgtcttg ggcgtcgcg gatcgccat tctgacaat 
      ataatggtc aaaaaatca ggtcaactg tttcacatc 
      gactttggc cacattttg ggccatttt aaggagaaa 
      tttgggata aaacgggaa agagtcccg tttgtgttg 
      acccacgac tttgaatat gtcataaac aaggggcag 
      aagacgacg cttgagttt aaaattttt caggagtat 
      tgtgagaag gcttttgtc attttgcga aaacatggg 
      agtttgatt ttgtcgctg tgtgcgatg atgatttcg 
      accggtttg cccgagttg agttcggag aaggacttg 
      aattacatc agagacact ttggccctg tcgaagtcg 
      gaggaggag gcgctaaac cactttagg aataaattc 
      gacgaagct ttgtcgaac tcgtggaag acttcgttg 
      aattgggcg tcgcataac ttagcgaag aataataaa 
      gcatga
    mRNA sequence
    • >TC011996 RA
      tgggcgatg ggtgtatga tgaaagcga ttcaaggag 
      attatttag aataaacag gttaaccac agcattatc 
      tgtgtttct cgaaaatga agcagcaaa attttccaa 
      ggaaatagc tcccgagcc ggaaattct aatctagaa 
      tcaaacagt gaattacca tgaagtagt gatgcgaga 
      aaacaatct ccaaatgcc tccatttcc ccgagaata 
      ctccccgga cttctggtc ctttgaccc agaagattc 
      cgtggaatt gacttgtct catgcctaa cgggattgt 
      gattctcct caaagtatc ctacaatgc caccctact 
      ggacatcaa agcggactt gtgggagga ggcccccaa 
      gtacccgct ccatggcaa gctccacga catgtcctc 
      gtatgtctt cacctgcat taatagcat ggcagagcg 
      ggaggagct gcatgacga gacgaggag aatctgcga 
      tgttaaacc tacttgcgc tgtactgaa aattgagtg 
      ccttgggaa tgaaccaga taacaagat caatgtcca 
      gatcgggca tttgatagg gaggagtgt taaagaatt 
      tgacgagtt gaataatcc cgagattaa cgatttccg 
      ttttcaaat gcggaaatt gggcgatga tattacgca 
      gaaacgctt gaaatatac gtggcttga aaagttgta 
      ttatcagtt tccgccgcg actgtgtcc caaaagcaa 
      aatctttcc ggaaacgct tgaccaaaa aaagaatat 
      taaaattac tacacgatt tgaagataa tgagaattc 
      ttttacgtt caatgtctc tccttacat ttacccaaa 
      caaattatt ggaaatgat tctcgcgaa aaggtccaa 
      cgttttgaa aatcagata cgaccgttg taacgatta 
      tgtattaaa agtgtgtgg ccaggacga gtatttagt 
      aggagatca ccaactcat tcaattcca atacatcca 
      agactcttt atctacaga cgtaactcc tattgtggt 
      cacagtgag agttaacaa agtcccagt cactcctga 
      taatcccta cgaatcgtt ggataattt cgaccacag 
      gagaatgcg gtcgactta ttcaacatt aacatggcg 
      taaaagagc ccacaagtc ggcttggga tattcagag 
      gaagtttag tgtgacttt gaacgcagt cagcggatt 
      gaattgtga tatgaggaa aacaaatga gattggcat 
      tcaggtggg attgtttca tgggggaaa gtcgctttg 
      cgaaagact taaagcttc catccaacc aataaacga 
      aaagaatga aagtttttt tcgtgagac tttagtttt 
      tgatatctt agtgtgcaa tatacccag aaatgcgaa 
      attgtgttt ttgcattta tgaagttcc taaatctag 
      taagttagt tagaacgaa aaaaggcaa agaatatgg 
      aaataataa ggagtctat tactcctat agcttgggc 
      taatacgac cgtttacga ttttaaaaa ccagctgag 
      aaatggcgc aatgacact ttacatgtg gacttttgc 
      caacgattt gatgtgtga ggatatatt taatgcttt 
      agggaatgt tgttaataa tccgaataa aagtgcaac 
      ggccttaac actacatct tatagatca aacgattta 
      ttatccaac gacggaagc aatgattaa ttttgctca 
      ggaaaacaa aagcaaata tattagtaa cagtattga 
      atacaatcc gacgaacat cgaacattg tcagaaaat 
      tcttaccga aaatgacaa tttgtttga ggttcatga 
      acaggatcg aaaaaatat ctggctgtt gaaagacta 
      ttggaaaaa acaaaatcc ggaagtcct tcctaagct 
      gttatcttc gattgactg ggatactcg tgaagtggt 
      gagcgatgt agctgcact attaaaaga ctggcctaa 
      gcttccgcc tgagaaagc attagaatt attagatta 
      tgcatatcc tgatcagag agttcggag ttttgcagt 
      tgaatgtct ggacgatgt cagtgacga ggacttact 
      tctttatct cttacaatt agtacaagc aatcaaaca 
      cgaaatgta tctagaatg tgatttagt aaaatttct 
      aataaaacg agcgctaaa taatcaaaa gatcggtca 
      ctacttatt ctggcatct caaatccga gctgcaagt 
      cccatctgt atcggtccg gttcggact tattctgga 
      agcctattg cagagggag tcaggaaca catgcctgt 
      gttgttaaa acagttaga atttctcga taagttgaa 
      gaacagtcg agaacacgt caagaacaa aagcaagga 
      aaaggctaa atcaaatct caaagatta cttaacaga 
      gtgccacat tgacgagac cgtgcaaaa cattaggag 
      cccgctgga ccccagttt ccggtgctc taaaataaa 
      attagacag atgtaggat tatggatag caaaatgcg 
      gccgttgtg gatcgttct ccagaatta cgatccgtt 
      cggagacga tgtttacat tattttcaa agacggcga 
      cgacttgag acaagatat gctcacgtt gcagatgtt 
      gaagattat ggatagatt gtggaagag cgaagggtt 
      ggatttaag attgagtcc gtacaattg catatcgtt 
      aggagatag ggccggtat gattgaagt agtgctcaa 
      cgcggagac cattgccaa catccagaa ggacaaggg 
      cgtgttttc ggccacttc ggccttccg caagggctc 
      gattttggc ttggttgag agaccacaa cccgacgga 
      gtcagctct caacaaggc catcaacga gttcaccct 
      ttcgtgtgc cggctattg cgtcgcgac gtacgtctt 
      gggcgtcgc ggatcgcca ttctgacaa tataatggt 
      caaaaaatc aggtcaact gtttcacat cgactttgg 
      ccacatttt gggccattt taaggagaa atttgggat 
      aaaacggga aagagtccc gtttgtgtt gacccacga 
      ctttgaata tgtcataaa caaggggca gaagacgac 
      gcttgagtt taaaatttt tcaggagta ttgtgagaa 
      ggcttttgt cattttgcg aaaacatgg gagtttgat 
      tttgtcgct gtgtgcgat gatgatttc gaccggttt 
      gcccgagtt gagttcgga gaaggactt gaattacat 
      cagagacac tttggccct gtcgaagtc ggaggagga 
      ggcgctaaa ccactttag gaataaatt cgacgaagc 
      tttgtcgaa ctcgtggaa gacttcgtt gaattgggc 
      gtcgcataa cttagcgaa gaataataa agcatgagc 
      taacgattt tgttgccaa tcaaagtgc caatataat 
      ttagtaata tcactagtg aattaatca aattttgta 
      catattgtt gtcgattta gttgtggaa ttttctatc 
      tggagcaat tttttaaat taggtcaag gatatcagg 
      gaaatggaa tttccgaaa tttagtacc taaagagcg 
      aatattttc attgagttt agggttaga cttgacgac 
      catgacaat agtcgtacc ttttgctta gtttgatat 
      ttctagtta aaattcggt tctcacaac aacgttaat 
      gccacgcaa tgcaatttc ttttaagtc gtactgatg 
      cgagtacta aaaatgttt ttttttata attttaatt 
      aggtcttag ctacaacac gcgtgattt gggttttac 
      aaaaggcac gcgcaaggt gcacattta tgatttatt 
      tattacgta actattcgt cttgatctt gggaactta 
      tatatttta cggttttat cgtttctgt tcatttaaa 
      atgttgtgt gaggtacac attaaaata tttgaatcc 
      caagagaaa aggagaata gcagccatt agacaaaat 
      gaatgtttc taatattat ttgtattac gctgattta 
      tgtacaaat atttaagtt tcattatat gtaaactat 
      atacaataa aatgtgtct ttattt