Status
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Gene information
General information
TC011996 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9066229..9079839
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011996
    iBeetle screen iB_02924

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    Pupal morphology
    head not bent - Number of animals showing the phenotype: 3

    wings shortened - Number of animals showing the phenotype: 10

    wings not closed - Number of animals showing the phenotype: 10

    Adult morphology
    wings not closed - Number of animals showing the phenotype: 5


    wings slightlyshortened - Number of animals showing the phenotype: 3


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011996 RA
      MRENNLQMP PFPREYSPD FWSFDPEDS VELTCLMPN 
GIVILLKVS YNATLLDIK ADLWEEAPK YPLHGKLHD 
MSSYVFTCI NSMAEREEL HDETRRICD VKPTCAVLK 
IECLGNEPD NKINVQIGH LIGRSVKEF DELNNPEIN 
DFRFQMRKL GDDITQKRL KYTWLEKLY YQFPPRLCP 
KSKIFPETL DQKKNIKIT TRFEDNENS FTFNVSPYI 
YPNKLLEMI LAKRSNVLK IRYDRCNDY VLKVCGQDE 
YLVGDHQLI QFQYIQDSL STDVTPIVV TVRVNKVPV 
TPDNPYESL DNFDHRRMR STYSTLTWR KRAHKSAWD 
IQRKFSVTL NAVSGLNCD MRKTNEIGI QVGLFHGGK 
SLCERLKAS IQPINEKNE SFFRETLVF DILVCNIPR 
NAKLCFCIY EVPKSSKLV RTKKGKEYG NNKESITPI 
AWANTTVYD FKNQLRNGA MTLYMWTFA NDLMCEDIF 
NALGNVVNN PNKSATALT LHLIDQTIY YPTTEAMIN 
FAQENKSKY ISNSIEYNP TNIEHCQKI LTENDNLFE 
VHEQDRKNI WLLKDYWKK QNPEVLPKL LSSIDWDTR 
EVVSDVAAL LKDWPKLPP EKALELLDY AYPDQRVRS 
FAVECLDDV SDEDLLLYL LQLVQAIKH EMYLECDLV 
KFLIKRALN NQKIGHYLF WHLKSELQV PSVSVRFGL 
ILEAYCRGS QEHMPVLLK QLEFLDKLK NSREHVKNK 
SKEKAKSNL KDYLTECHI DETVQNIRS PLDPSFRCS 
KIKLDRCRI MDSKMRPLW IVLQNYDPF GDDVYIIFK 
DGDDLRQDM LTLQMLKIM DRLWKSEGL DLRLSPYNC 
ISLGDRAGM IEVVLNAET IANIQKDKG VFSATSAFR 
KGSILAWLR DHNPTESAL NKAINEFTL SCAGYCVAT 
YVLGVADRH SDNIMVKKS GQLFHIDFG HILGHFKEK 
FGIKRERVP FVLTHDFEY VINKGQKTT LEFKIFQEY 
CEKAFVILR KHGSLILSL CAMMISTGL PELSSEKDL 
NYIRDTLAL SKSEEEALN HFRNKFDEA LSNSWKTSL 
NWASHNLAK NNKA
    CDS sequence
    • >TC011996 RA
      atgcgagaa aacaatctc caaatgcct ccatttccc 
cgagaatac tccccggac ttctggtcc tttgaccca 
gaagattcc gtggaattg acttgtctc atgcctaac 
gggattgtg attctcctc aaagtatcc tacaatgcc 
accctactg gacatcaaa gcggacttg tgggaggag 
gcccccaag tacccgctc catggcaag ctccacgac 
atgtcctcg tatgtcttc acctgcatt aatagcatg 
gcagagcgg gaggagctg catgacgag acgaggaga 
atctgcgat gttaaacct acttgcgct gtactgaaa 
attgagtgc cttgggaat gaaccagat aacaagatc 
aatgtccag atcgggcat ttgataggg aggagtgtt 
aaagaattt gacgagttg aataatccc gagattaac 
gatttccgt tttcaaatg cggaaattg ggcgatgat 
attacgcag aaacgcttg aaatatacg tggcttgaa 
aagttgtat tatcagttt ccgccgcga ctgtgtccc 
aaaagcaaa atctttccg gaaacgctt gaccaaaaa 
aagaatatt aaaattact acacgattt gaagataat 
gagaattct tttacgttc aatgtctct ccttacatt 
tacccaaac aaattattg gaaatgatt ctcgcgaaa 
aggtccaac gttttgaaa atcagatac gaccgttgt 
aacgattat gtattaaaa gtgtgtggc caggacgag 
tatttagta ggagatcac caactcatt caattccaa 
tacatccaa gactcttta tctacagac gtaactcct 
attgtggtc acagtgaga gttaacaaa gtcccagtc 
actcctgat aatccctac gaatcgttg gataatttc 
gaccacagg agaatgcgg tcgacttat tcaacatta 
acatggcgt aaaagagcc cacaagtcg gcttgggat 
attcagagg aagtttagt gtgactttg aacgcagtc 
agcggattg aattgtgat atgaggaaa acaaatgag 
attggcatt caggtggga ttgtttcat gggggaaag 
tcgctttgc gaaagactt aaagcttcc atccaacca 
ataaacgaa aagaatgaa agttttttt cgtgagact 
ttagttttt gatatctta gtgtgcaat atacccaga 
aatgcgaaa ttgtgtttt tgcatttat gaagttcct 
aaatctagt aagttagtt agaacgaaa aaaggcaaa 
gaatatgga aataataag gagtctatt actcctata 
gcttgggct aatacgacc gtttacgat tttaaaaac 
cagctgaga aatggcgca atgacactt tacatgtgg 
acttttgcc aacgatttg atgtgtgag gatatattt 
aatgcttta gggaatgtt gttaataat ccgaataaa 
agtgcaacg gccttaaca ctacatctt atagatcaa 
acgatttat tatccaacg acggaagca atgattaat 
tttgctcag gaaaacaaa agcaaatat attagtaac 
agtattgaa tacaatccg acgaacatc gaacattgt 
cagaaaatt cttaccgaa aatgacaat ttgtttgag 
gttcatgaa caggatcga aaaaatatc tggctgttg 
aaagactat tggaaaaaa caaaatccg gaagtcctt 
cctaagctg ttatcttcg attgactgg gatactcgt 
gaagtggtg agcgatgta gctgcacta ttaaaagac 
tggcctaag cttccgcct gagaaagca ttagaatta 
ttagattat gcatatcct gatcagaga gttcggagt 
tttgcagtt gaatgtctg gacgatgtc agtgacgag 
gacttactt ctttatctc ttacaatta gtacaagca 
atcaaacac gaaatgtat ctagaatgt gatttagta 
aaatttcta ataaaacga gcgctaaat aatcaaaag 
atcggtcac tacttattc tggcatctc aaatccgag 
ctgcaagtc ccatctgta tcggtccgg ttcggactt 
attctggaa gcctattgc agagggagt caggaacac 
atgcctgtg ttgttaaaa cagttagaa tttctcgat 
aagttgaag aacagtcga gaacacgtc aagaacaaa 
agcaaggaa aaggctaaa tcaaatctc aaagattac 
ttaacagag tgccacatt gacgagacc gtgcaaaac 
attaggagc ccgctggac cccagtttc cggtgctct 
aaaataaaa ttagacaga tgtaggatt atggatagc 
aaaatgcgg ccgttgtgg atcgttctc cagaattac 
gatccgttc ggagacgat gtttacatt attttcaaa 
gacggcgac gacttgaga caagatatg ctcacgttg 
cagatgttg aagattatg gatagattg tggaagagc 
gaagggttg gatttaaga ttgagtccg tacaattgc 
atatcgtta ggagatagg gccggtatg attgaagta 
gtgctcaac gcggagacc attgccaac atccagaag 
gacaagggc gtgttttcg gccacttcg gccttccgc 
aagggctcg attttggct tggttgaga gaccacaac 
ccgacggag tcagctctc aacaaggcc atcaacgag 
ttcaccctt tcgtgtgcc ggctattgc gtcgcgacg 
tacgtcttg ggcgtcgcg gatcgccat tctgacaat 
ataatggtc aaaaaatca ggtcaactg tttcacatc 
gactttggc cacattttg ggccatttt aaggagaaa 
tttgggata aaacgggaa agagtcccg tttgtgttg 
acccacgac tttgaatat gtcataaac aaggggcag 
aagacgacg cttgagttt aaaattttt caggagtat 
tgtgagaag gcttttgtc attttgcga aaacatggg 
agtttgatt ttgtcgctg tgtgcgatg atgatttcg 
accggtttg cccgagttg agttcggag aaggacttg 
aattacatc agagacact ttggccctg tcgaagtcg 
gaggaggag gcgctaaac cactttagg aataaattc 
gacgaagct ttgtcgaac tcgtggaag acttcgttg 
aattgggcg tcgcataac ttagcgaag aataataaa 
gcatga
    mRNA sequence
    • >TC011996 RA
      tgggcgatg ggtgtatga tgaaagcga ttcaaggag 
attatttag aataaacag gttaaccac agcattatc 
tgtgtttct cgaaaatga agcagcaaa attttccaa 
ggaaatagc tcccgagcc ggaaattct aatctagaa 
tcaaacagt gaattacca tgaagtagt gatgcgaga 
aaacaatct ccaaatgcc tccatttcc ccgagaata 
ctccccgga cttctggtc ctttgaccc agaagattc 
cgtggaatt gacttgtct catgcctaa cgggattgt 
gattctcct caaagtatc ctacaatgc caccctact 
ggacatcaa agcggactt gtgggagga ggcccccaa 
gtacccgct ccatggcaa gctccacga catgtcctc 
gtatgtctt cacctgcat taatagcat ggcagagcg 
ggaggagct gcatgacga gacgaggag aatctgcga 
tgttaaacc tacttgcgc tgtactgaa aattgagtg 
ccttgggaa tgaaccaga taacaagat caatgtcca 
gatcgggca tttgatagg gaggagtgt taaagaatt 
tgacgagtt gaataatcc cgagattaa cgatttccg 
ttttcaaat gcggaaatt gggcgatga tattacgca 
gaaacgctt gaaatatac gtggcttga aaagttgta 
ttatcagtt tccgccgcg actgtgtcc caaaagcaa 
aatctttcc ggaaacgct tgaccaaaa aaagaatat 
taaaattac tacacgatt tgaagataa tgagaattc 
ttttacgtt caatgtctc tccttacat ttacccaaa 
caaattatt ggaaatgat tctcgcgaa aaggtccaa 
cgttttgaa aatcagata cgaccgttg taacgatta 
tgtattaaa agtgtgtgg ccaggacga gtatttagt 
aggagatca ccaactcat tcaattcca atacatcca 
agactcttt atctacaga cgtaactcc tattgtggt 
cacagtgag agttaacaa agtcccagt cactcctga 
taatcccta cgaatcgtt ggataattt cgaccacag 
gagaatgcg gtcgactta ttcaacatt aacatggcg 
taaaagagc ccacaagtc ggcttggga tattcagag 
gaagtttag tgtgacttt gaacgcagt cagcggatt 
gaattgtga tatgaggaa aacaaatga gattggcat 
tcaggtggg attgtttca tgggggaaa gtcgctttg 
cgaaagact taaagcttc catccaacc aataaacga 
aaagaatga aagtttttt tcgtgagac tttagtttt 
tgatatctt agtgtgcaa tatacccag aaatgcgaa 
attgtgttt ttgcattta tgaagttcc taaatctag 
taagttagt tagaacgaa aaaaggcaa agaatatgg 
aaataataa ggagtctat tactcctat agcttgggc 
taatacgac cgtttacga ttttaaaaa ccagctgag 
aaatggcgc aatgacact ttacatgtg gacttttgc 
caacgattt gatgtgtga ggatatatt taatgcttt 
agggaatgt tgttaataa tccgaataa aagtgcaac 
ggccttaac actacatct tatagatca aacgattta 
ttatccaac gacggaagc aatgattaa ttttgctca 
ggaaaacaa aagcaaata tattagtaa cagtattga 
atacaatcc gacgaacat cgaacattg tcagaaaat 
tcttaccga aaatgacaa tttgtttga ggttcatga 
acaggatcg aaaaaatat ctggctgtt gaaagacta 
ttggaaaaa acaaaatcc ggaagtcct tcctaagct 
gttatcttc gattgactg ggatactcg tgaagtggt 
gagcgatgt agctgcact attaaaaga ctggcctaa 
gcttccgcc tgagaaagc attagaatt attagatta 
tgcatatcc tgatcagag agttcggag ttttgcagt 
tgaatgtct ggacgatgt cagtgacga ggacttact 
tctttatct cttacaatt agtacaagc aatcaaaca 
cgaaatgta tctagaatg tgatttagt aaaatttct 
aataaaacg agcgctaaa taatcaaaa gatcggtca 
ctacttatt ctggcatct caaatccga gctgcaagt 
cccatctgt atcggtccg gttcggact tattctgga 
agcctattg cagagggag tcaggaaca catgcctgt 
gttgttaaa acagttaga atttctcga taagttgaa 
gaacagtcg agaacacgt caagaacaa aagcaagga 
aaaggctaa atcaaatct caaagatta cttaacaga 
gtgccacat tgacgagac cgtgcaaaa cattaggag 
cccgctgga ccccagttt ccggtgctc taaaataaa 
attagacag atgtaggat tatggatag caaaatgcg 
gccgttgtg gatcgttct ccagaatta cgatccgtt 
cggagacga tgtttacat tattttcaa agacggcga 
cgacttgag acaagatat gctcacgtt gcagatgtt 
gaagattat ggatagatt gtggaagag cgaagggtt 
ggatttaag attgagtcc gtacaattg catatcgtt 
aggagatag ggccggtat gattgaagt agtgctcaa 
cgcggagac cattgccaa catccagaa ggacaaggg 
cgtgttttc ggccacttc ggccttccg caagggctc 
gattttggc ttggttgag agaccacaa cccgacgga 
gtcagctct caacaaggc catcaacga gttcaccct 
ttcgtgtgc cggctattg cgtcgcgac gtacgtctt 
gggcgtcgc ggatcgcca ttctgacaa tataatggt 
caaaaaatc aggtcaact gtttcacat cgactttgg 
ccacatttt gggccattt taaggagaa atttgggat 
aaaacggga aagagtccc gtttgtgtt gacccacga 
ctttgaata tgtcataaa caaggggca gaagacgac 
gcttgagtt taaaatttt tcaggagta ttgtgagaa 
ggcttttgt cattttgcg aaaacatgg gagtttgat 
tttgtcgct gtgtgcgat gatgatttc gaccggttt 
gcccgagtt gagttcgga gaaggactt gaattacat 
cagagacac tttggccct gtcgaagtc ggaggagga 
ggcgctaaa ccactttag gaataaatt cgacgaagc 
tttgtcgaa ctcgtggaa gacttcgtt gaattgggc 
gtcgcataa cttagcgaa gaataataa agcatgagc 
taacgattt tgttgccaa tcaaagtgc caatataat 
ttagtaata tcactagtg aattaatca aattttgta 
catattgtt gtcgattta gttgtggaa ttttctatc 
tggagcaat tttttaaat taggtcaag gatatcagg 
gaaatggaa tttccgaaa tttagtacc taaagagcg 
aatattttc attgagttt agggttaga cttgacgac 
catgacaat agtcgtacc ttttgctta gtttgatat 
ttctagtta aaattcggt tctcacaac aacgttaat 
gccacgcaa tgcaatttc ttttaagtc gtactgatg 
cgagtacta aaaatgttt ttttttata attttaatt 
aggtcttag ctacaacac gcgtgattt gggttttac 
aaaaggcac gcgcaaggt gcacattta tgatttatt 
tattacgta actattcgt cttgatctt gggaactta 
tatatttta cggttttat cgtttctgt tcatttaaa 
atgttgtgt gaggtacac attaaaata tttgaatcc 
caagagaaa aggagaata gcagccatt agacaaaat 
gaatgtttc taatattat ttgtattac gctgattta 
tgtacaaat atttaagtt tcattatat gtaaactat 
atacaataa aatgtgtct ttattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY