Status
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Gene information
General information
TC010681 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7818610..7839575
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010681
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02923

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010681 RA
      MEPRAAAAL QFNASRGHK MDAVPKRLK PEPEEAEAG 
      PRAPSPSFR RENSTRSLR SAYVGDAKT SSESGSHLS 
      KPLSDAASV RSWASVGMG STDGRKMIV RRVPTSPVE 
      LFNIVNPPT PPEEYLYDG ESDDGSGDD EDSVYIKPR 
      RQTWSNKLQ FVLACVGYS VGLGSVWRF PYLCYKSGG 
      GVFLIPYAI IMIVCGVPM LYMELSVGQ YTGRGPIGA 
      LGHLCPLLK GTGLGSVVI SFLMSTYYS VIIAYGIYY 
      FFTSFKAKQ PWEDCSHRW NTDECWVPG KSSPSIAKP 
      NDSQTPAEQ FYDKKVLQI GDGIEDFDS LRWELVACL 
      LCAWVMVYF AIWKSIKSS AKVRYFTAT LPFLLIIVF 
      LAKSLTLEG ADKGMRYFF KPKFQLLLD AKVWVNAAA 
      QTFNSMGIA FGSMISFAS YSKYNNNIL HDTLAVSFV 
      NAITSLLVG IFAFATIGN IASEQGTSI EAVIDDGPG 
      LIFVVYPQA MAKMPAAQL WAVLFFFML ICLALNSQF 
      AIVEVVVTS IQDGFPAWI KKHLMCHEV LVLIICVVS 
      LICGLPNVT QGGIYFFQL IDHYAASIS IMYLAFFEV 
      IAIAWFYGV TRLSKNVKT MTGRQPSLY FKFCWLIAT 
      PLMIFSVWV FCMIDYESP TYNNGEYHY PIWAIVIGW 
      IISALSILC IPIYMVYVF MKSPGNTFM EKLKSSLKS 
      DLLDKCPKC EEFDCECVV KEGEIEPML VMHKPDSKD 
      SNSTSVPFY IPLSRVPTV HKFDLEKVA VQRPLFLNI 
      DKVQRHKLD NSKEDVLKN SVQNGTIPE EDRLQADDL 
      ASDSTNTEV QQEKDYSSH SNIHLVAAD AANQLKTCE 
      KGDGEVQAN GGECDGAKE
    CDS sequence
    • >TC010681 RA
      atggagcct agagccgcc gccgctctc cagttcaac 
      gcgagccga ggccacaaa atggacgcc gtcccgaag 
      cgcctcaag ccggagccc gaggaggcc gaagcgggc 
      ccgcgggcg ccgtcgccg agcttccgg cgcgagaac 
      agcaccagg tccctgcgg tcggcctac gtcggcgac 
      gccaagacc agcagcgag tccggcagc cacctctcg 
      aagccgctc tccgacgcg gcctcggtg cgctcctgg 
      gcctccgtg gggatgggc tccaccgac ggcaggaag 
      atgatcgtg cggagggtg cccacgtcg cccgttgaa 
      ctcttcaac atcgtcaat ccgccgacg ccccccgaa 
      gaatattta tacgacggc gagagtgac gacggcagt 
      ggcgatgac gaagacagc gtctatatt aaaccacga 
      agacaaaca tggtccaac aagctccaa ttcgtcctg 
      gcctgtgtg ggctacagc gtcggccta ggatcggtc 
      tggaggttt ccgtattta tgttacaaa agtggtgga 
      ggagtgttt ctcatccct tacgcgatc ataatgata 
      gtctgcggc gtccccatg ctgtacatg gagttgagc 
      gtgggccaa tacaccggc cgcggcccc atcggcgcc 
      ctgggccac ctgtgcccc cttctgaaa ggaaccggt 
      ctaggtagc gtagtcatc tcatttctc atgtcaacc 
      tactacagt gtgataata gcgtacgga atttactat 
      ttcttcact tcgttcaaa gcgaaacag ccgtgggag 
      gactgctcc caccgctgg aacacggac gagtgctgg 
      gtccccggc aagtcctcc cccagcatc gccaagccc 
      aacgacagc cagaccccc gccgagcag ttctacgac 
      aaaaaggtc ctccagatc ggcgacggc atcgaggac 
      ttcgacagc ctgcgctgg gagctggtc gcctgcctg 
      ctgtgcgcc tgggtgatg gtctatttc gcgatctgg 
      aagagtatt aagtcctcg gccaaagtg cggtatttc 
      accgccacg ttgcccttc ctgctcatc attgtcttc 
      ctcgccaag agtctgacg ctggagggg gccgacaaa 
      ggcatgcgc tattttttc aagcccaag ttccagctg 
      ctcctagat gccaaagtg tgggtcaat gccgccgcc 
      cagaccttc aattccatg ggcatcgcc tttggttcg 
      atgatctcg ttcgccagc tacagcaaa tacaacaac 
      aacatcctg cacgacact ctggccgtg tcgtttgtc 
      aatgccata acgagcctt ttggttggg attttcgcg 
      ttcgcgacc atcgggaac atcgcctcc gagcaggga 
      acgtcgatc gaagccgtc atcgacgat ggtcccggc 
      ctgattttc gtggtgtac ccccaggcc atggccaaa 
      atgccggca gcgcagctc tgggccgtc ctcttcttc 
      ttcatgctg atttgcctg gctttgaac agccaattt 
      gcgattgtt gaagtcgtg gtgacttcc attcaggac 
      ggcttcccg gcgtggatc aagaagcac ctgatgtgc 
      cacgaggtc ctggtgctg atcatctgc gttgtctcg 
      ctcatctgc ggactgccg aacgtcacg cagggcgga 
      atttacttc ttccagttg atagaccac tacgccgcg 
      tcgatttcc ataatgtat ctcgcgttt ttcgaagtg 
      attgcaatt gcgtggttt tacggcgtg acgagattg 
      agcaagaac gtgaagaca atgactggc cgccagcca 
      agtctctat ttcaaattc tgttggctg atcgcaacc 
      ccgcttatg attttctcc gtctgggtc ttctgcatg 
      atcgactac gagtcaccg acttacaac aacggggaa 
      taccactac ccgatttgg gccattgtg atcggctgg 
      atcatctcg gctttgtca attctatgc attccgatt 
      tacatggtg tacgtgttt atgaaatct cccggcaat 
      acgttcatg gagaaattg aaaagtagt ttgaagtcg 
      gacctcctc gacaagtgc cccaagtgc gaggagttc 
      gactgcgaa tgcgtggtg aaagagggc gagatcgag 
      ccgatgttg gtgatgcac aagccggat tcgaaagac 
      tccaactcc acgagcgtg cccttctac attccccta 
      agccgcgtc cccacggtc cataaattc gatttggag 
      aaagtggcc gtgcaaagg cccctcttt ttgaacatc 
      gataaagtc cagcgccac aaactggac aattcgaag 
      gaagacgtc ctcaaaaat agtgttcaa aacgggacg 
      atacccgaa gaagacagg ttgcaggcc gatgatcta 
      gcgtccgat agtacaaat accgaagtt cagcaagaa 
      aaagattac tcgtctcat tcaaacata caccttgtg 
      gcggctgat gcagcgaat cagttgaaa acgtgtgag 
      aagggagat ggggaagtg caagcgaat ggaggtgaa 
      tgtgatggc gccaaagag tag
    mRNA sequence
    • >TC010681 RA
      tccgtttca aatcttgaa aacccagct tattaataa 
      aatatcgcg ataataacc gcgagtggg aacccgaga 
      ttggcgttc cacaccgaa ttgtcagtc ttgccgcgg 
      cagcgctac tcccagcag ttcgccagt cgcctctcc 
      catccgagc catgaagtg cgcccgtcg taatgacag 
      tgtaggccc aaattgctc ccaatcttc caataatgg 
      agcctagag ccgccgccg ctctccagt tcaacgcga 
      gccgaggcc acaaaatgg acgccgtcc cgaagcgcc 
      tcaagccgg agcccgagg aggccgaag cgggcccgc 
      gggcgccgt cgccgagct tccggcgcg agaacagca 
      ccaggtccc tgcggtcgg cctacgtcg gcgacgcca 
      agaccagca gcgagtccg gcagccacc tctcgaagc 
      cgctctccg acgcggcct cggtgcgct cctgggcct 
      ccgtgggga tgggctcca ccgacggca ggaagatga 
      tcgtgcgga gggtgccca cgtcgcccg ttgaactct 
      tcaacatcg tcaatccgc cgacgcccc ccgaagaat 
      atttatacg acggcgaga gtgacgacg gcagtggcg 
      atgacgaag acagcgtct atattaaac cacgaagac 
      aaacatggt ccaacaagc tccaattcg tcctggcct 
      gtgtgggct acagcgtcg gcctaggat cggtctgga 
      ggtttccgt atttatgtt acaaaagtg gtggaggag 
      tgtttctca tcccttacg cgatcataa tgatagtct 
      gcggcgtcc ccatgctgt acatggagt tgagcgtgg 
      gccaataca ccggccgcg gccccatcg gcgccctgg 
      gccacctgt gcccccttc tgaaaggaa ccggtctag 
      gtagcgtag tcatctcat ttctcatgt caacctact 
      acagtgtga taatagcgt acggaattt actatttct 
      tcacttcgt tcaaagcga aacagccgt gggaggact 
      gctcccacc gctggaaca cggacgagt gctgggtcc 
      ccggcaagt cctccccca gcatcgcca agcccaacg 
      acagccaga cccccgccg agcagttct acgacaaaa 
      aggtcctcc agatcggcg acggcatcg aggacttcg 
      acagcctgc gctgggagc tggtcgcct gcctgctgt 
      gcgcctggg tgatggtct atttcgcga tctggaaga 
      gtattaagt cctcggcca aagtgcggt atttcaccg 
      ccacgttgc ccttcctgc tcatcattg tcttcctcg 
      ccaagagtc tgacgctgg agggggccg acaaaggca 
      tgcgctatt ttttcaagc ccaagttcc agctgctcc 
      tagatgcca aagtgtggg tcaatgccg ccgcccaga 
      ccttcaatt ccatgggca tcgcctttg gttcgatga 
      tctcgttcg ccagctaca gcaaataca acaacaaca 
      tcctgcacg acactctgg ccgtgtcgt ttgtcaatg 
      ccataacga gccttttgg ttgggattt tcgcgttcg 
      cgaccatcg ggaacatcg cctccgagc agggaacgt 
      cgatcgaag ccgtcatcg acgatggtc ccggcctga 
      ttttcgtgg tgtaccccc aggccatgg ccaaaatgc 
      cggcagcgc agctctggg ccgtcctct tcttcttca 
      tgctgattt gcctggctt tgaacagcc aatttgcga 
      ttgttgaag tcgtggtga cttccattc aggacggct 
      tcccggcgt ggatcaaga agcacctga tgtgccacg 
      aggtcctgg tgctgatca tctgcgttg tctcgctca 
      tctgcggac tgccgaacg tcacgcagg gcggaattt 
      acttcttcc agttgatag accactacg ccgcgtcga 
      tttccataa tgtatctcg cgtttttcg aagtgattg 
      caattgcgt ggttttacg gcgtgacga gattgagca 
      agaacgtga agacaatga ctggccgcc agccaagtc 
      tctatttca aattctgtt ggctgatcg caaccccgc 
      ttatgattt tctccgtct gggtcttct gcatgatcg 
      actacgagt caccgactt acaacaacg gggaatacc 
      actacccga tttgggcca ttgtgatcg gctggatca 
      tctcggctt tgtcaattc tatgcattc cgatttaca 
      tggtgtacg tgtttatga aatctcccg gcaatacgt 
      tcatggaga aattgaaaa gtagtttga agtcggacc 
      tcctcgaca agtgcccca agtgcgagg agttcgact 
      gcgaatgcg tggtgaaag agggcgaga tcgagccga 
      tgttggtga tgcacaagc cggattcga aagactcca 
      actccacga gcgtgccct tctacattc ccctaagcc 
      gcgtcccca cggtccata aattcgatt tggagaaag 
      tggccgtgc aaaggcccc tctttttga acatcgata 
      aagtccagc gccacaaac tggacaatt cgaaggaag 
      acgtcctca aaaatagtg ttcaaaacg ggacgatac 
      ccgaagaag acaggttgc aggccgatg atctagcgt 
      ccgatagta caaataccg aagttcagc aagaaaaag 
      attactcgt ctcattcaa acatacacc ttgtggcgg 
      ctgatgcag cgaatcagt tgaaaacgt gtgagaagg 
      gagatgggg aagtgcaag cgaatggag gtgaatgtg 
      atggcgcca aagagtagt ggtttcgcg tgaccaatt 
      aacattccc ttaattaag ctcatgatt agtctagtt 
      gtaaattca ttataaatg aaacaattg tgatatagg 
      tagagtttt aacttaaat tggacctaa atacgtaat 
      gtatcaaat ttcaaacaa taaatcagc aatcacgag 
      attttcatt tttcataaa catgttttt caaattttt 
      atgtcgaga cattgacgc aaacgtgca gttggcgtt 
      ccgcgcaat aaataactt tagcatac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY