Status
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Gene information
General information
TC010651 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 8219097..8225384
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010651
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02922

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Few larvae on slide, No observable phenotype found.

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010651 RA
      MSRVKSRQN VEICGDCGA PDATWASIN KGILLCTQC 
      CSIHRSLGR HISQVKSLQ KGSWNPNQL AMVCTLNNN 
      GANSIWEHN LLENNGKLM KKKPNPKDA ISVKSEFIK 
      AKHLQCAYT FRDNATYDE GLENELGKQ LHASVRTPN 
      LETSFRLLV QGADPNYFH DERGTTPLH IAAKTEQKL 
      QVELLLVYG ADPTFPDAH GKTPIDYAK KNVNKDLLN 
      RLIESQYEV TDAFIQYLT HKRPDHQNG IHFILPQGG 
      FESNPNSLT KLQKMKNAL FEQLAMDVY DEIDRRETE 
      AIWLSCVDA IDLNVVPFL PVDSTMSKP RNQGRQKLA 
      RFSTPELKS LVFDILIDT QRRQLLSET GPNRTHLRE 
      YSPVSDDDP LYDSVAPDE DYALPPIQE NEKPQTDTV 
      EQLTKQLKA SDSTICDLK AEVNQLKAH VESLKCENI 
      ELKTRLSQC KVPETNNSI NGDEFSSLQ PIISNGDVD 
      LRQNRKNQR PSSMYETRE GLKTPNWQL LKNQIKQND 
      QLRNATQSL YGTAQDKQT VFECTELIT RSIQQLCKS 
      IQDRDKEDC VDSGEKVKF AVLRLATIL PKETEKKSI 
      KPMCDILPH LLAECYSLQ TAHKEGDAA KIERHLTKI 
      RELSFYLAK HTKDILTRY SSAQ
    CDS sequence
    • >TC010651 RA
      atgtctcgc gttaagtcg cgccagaat gtcgaaatt 
      tgtggcgac tgtggtgcc cctgatgcg acttgggct 
      tccataaat aagggcatt ctgctctgt acgcaatgc 
      tgcagcata caccgcagt ctcgggcgg cacatctca 
      caagtgaag tcgctccaa aagggcagt tggaacccg 
      aaccaatta gctatggtc tgtacgttg aataataac 
      ggggctaac agcatatgg gagcacaat ctgctggaa 
      aacaatggg aaattgatg aaaaagaag cccaatccc 
      aaagatgcc atcagtgtc aagagcgag tttattaag 
      gcgaagcac ctgcagtgc gcttatacg ttccgcgac 
      aacgccacc tatgacgag gggcttgaa aatgagctg 
      gggaagcag ctgcacgct agcgtgaga acaccaaat 
      ttggagact tcgtttcgg ttacttgtg caaggagct 
      gatccgaat tattttcac gatgaaagg ggcaccaca 
      cccctacat atagccgct aaaactgag cagaagtta 
      caagtggaa cttttgtta gtttacggt gctgatcca 
      acattccca gatgcccac ggcaaaaca cccatcgat 
      tatgcaaag aaaaatgtc aataaagat ttgttgaac 
      agattaata gaaagtcaa tatgaagtt acggatgcg 
      ttcatacag tatttgact cacaagagg ccggatcac 
      caaaatggc atacatttt attttgcca caaggcggc 
      tttgaatca aatcctaat tccctcaca aagttgcaa 
      aagatgaaa aatgcgctc tttgaacag ttggccatg 
      gacgtgtat gatgaaatt gacaggcgg gaaacagaa 
      gcaatttgg ttgagttgc gtggacgca attgatcta 
      aacgtggtc cctttcctt cccgtggac tcaacaatg 
      tcaaaaccg cgaaatcaa ggtcgtcaa aaattggcc 
      cgtttttca actcctgaa ttaaaaagt ctagttttt 
      gatatctta attgacaca caaagacgc cagttgtta 
      tccgagaca ggccccaat cgaacgcac cttcgtgaa 
      tattcgccc gtttcggac gacgatccg ctttacgac 
      tctgtggca cctgacgaa gattacgca ttgccgcca 
      attcaagaa aacgaaaaa ccacaaacc gacaccgtc 
      gaacagctc acaaaacaa ttgaaggcg tcggacagc 
      accatctgt gatctcaaa gcggaagtg aatcaatta 
      aaagcgcac gtcgagagt ttgaagtgc gaaaatatc 
      gaactgaaa acgcgtttg tcccagtgc aaagttcca 
      gagaccaat aacagtatt aacggcgat gagtttagt 
      tcgctacaa ccgataata tcgaacggt gatgttgat 
      ttgcgacaa aatcgtaaa aatcagcgt ccgtcgagc 
      atgtacgag actcgagaa ggcctgaaa acaccaaat 
      tggcaattg ttgaaaaat caaatcaag caaaacgac 
      caattacgc aacgccacg caaagtttg tacgggacg 
      gctcaagac aaacaaacc gtttttgaa tgcaccgaa 
      ttgataaca cgaagtatt caacagttg tgtaaaagt 
      attcaggat cgggataaa gaagattgt gtcgatagt 
      ggggaaaaa gtcaagttt gctgtactc agacttgcc 
      acgatcttg ccgaaagaa acagagaag aaaagtatc 
      aagcccatg tgcgatatt ttaccgcat ttgttggcg 
      gagtgttac tcgttacaa actgcccac aaggagggc 
      gatgccgct aaaatagaa cgtcatctt actaaaata 
      agggaattg tcattttac ttagctaag cacacgaag 
      gacatacta acgagatat tcatccgcg caatga
    mRNA sequence
    • >TC010651 RA
      acaaatatt attaatcaa acatttgtt tgtttatat 
      tttaacact aattggtga tagtggcgt aacataaac 
      actcgtttg tggcgatgt ctcgcgtta agtcgcgcc 
      agaatgtcg aaatttgtg gcgactgtg gtgcccctg 
      atgcgactt gggcttcca taaataagg gcattctgc 
      tctgtacgc aatgctgca gcatacacc gcagtctcg 
      ggcggcaca tctcacaag tgaagtcgc tccaaaagg 
      gcagttgga acccgaacc aattagcta tggtctgta 
      cgttgaata ataacgggg ctaacagca tatgggagc 
      acaatctgc tggaaaaca atgggaaat tgatgaaaa 
      agaagccca atcccaaag atgccatca gtgtcaaga 
      gcgagttta ttaaggcga agcacctgc agtgcgctt 
      atacgttcc gcgacaacg ccacctatg acgaggggc 
      ttgaaaatg agctgggga agcagctgc acgctagcg 
      tgagaacac caaatttgg agacttcgt ttcggttac 
      ttgtgcaag gagctgatc cgaattatt ttcacgatg 
      aaaggggca ccacacccc tacatatag ccgctaaaa 
      ctgagcaga agttacaag tggaacttt tgttagttt 
      acggtgctg atccaacat tcccagatg cccacggca 
      aaacaccca tcgattatg caaagaaaa atgtcaata 
      aagatttgt tgaacagat taatagaaa gtcaatatg 
      aagttacgg atgcgttca tacagtatt tgactcaca 
      agaggccgg atcaccaaa atggcatac attttattt 
      tgccacaag gcggctttg aatcaaatc ctaattccc 
      tcacaaagt tgcaaaaga tgaaaaatg cgctctttg 
      aacagttgg ccatggacg tgtatgatg aaattgaca 
      ggcgggaaa cagaagcaa tttggttga gttgcgtgg 
      acgcaattg atctaaacg tggtccctt tccttcccg 
      tggactcaa caatgtcaa aaccgcgaa atcaaggtc 
      gtcaaaaat tggcccgtt tttcaactc ctgaattaa 
      aaagtctag tttttgata tcttaattg acacacaaa 
      gacgccagt tgttatccg agacaggcc ccaatcgaa 
      cgcaccttc gtgaatatt cgcccgttt cggacgacg 
      atccgcttt acgactctg tggcacctg acgaagatt 
      acgcattgc cgccaattc aagaaaacg aaaaaccac 
      aaaccgaca ccgtcgaac agctcacaa aacaattga 
      aggcgtcgg acagcacca tctgtgatc tcaaagcgg 
      aagtgaatc aattaaaag cgcacgtcg agagtttga 
      agtgcgaaa atatcgaac tgaaaacgc gtttgtccc 
      agtgcaaag ttccagaga ccaataaca gtattaacg 
      gcgatgagt ttagttcgc tacaaccga taatatcga 
      acggtgatg ttgatttgc gacaaaatc gtaaaaatc 
      agcgtccgt cgagcatgt acgagactc gagaaggcc 
      tgaaaacac caaattggc aattgttga aaaatcaaa 
      tcaagcaaa acgaccaat tacgcaacg ccacgcaaa 
      gtttgtacg ggacggctc aagacaaac aaaccgttt 
      ttgaatgca ccgaattga taacacgaa gtattcaac 
      agttgtgta aaagtattc aggatcggg ataaagaag 
      attgtgtcg atagtgggg aaaaagtca agtttgctg 
      tactcagac ttgccacga tcttgccga aagaaacag 
      agaagaaaa gtatcaagc ccatgtgcg atattttac 
      cgcatttgt tggcggagt gttactcgt tacaaactg 
      cccacaagg agggcgatg ccgctaaaa tagaacgtc 
      atcttacta aaataaggg aattgtcat tttacttag 
      ctaagcaca cgaaggaca tactaacga gatattcat 
      ccgcgcaat gattaattc cagtgtttt gattcctta 
      tttattacg aataacagt aagctatta attgtttct 
      caaaagttt taacaagtg ttatgatgg aatgtgcaa 
      tttgaataa ttgttttta aaaatgttg ctccagatt 
      gttttaaat tttaactta ttgcgattg ttcgtagct 
      aatagactg attgtatct gtttatcca tcattgaat 
      tgacataag gtgctaaaa tctacatca ctgttgcca 
      gtttatctt ccat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY