| General information | |||
| TC012455 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 7756236..7778628 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC012455 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02921 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
Days post injection
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
Days post injection
larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
Days post injection
- >TC012455 RA
MSEQLKVGQ KVKVTGKDV QGVIAFIGP TSFAADTWI GLKLDEPKG KNNGSVQGV EYFKCEDKH GLFVKPSQV IPLDEHGKP LRDAGEETP KARARPSSS RLSLASSRQ SLADSRQSL SGSRQSLAG SRQSLMGSR SHLASPIVE KSDAGDHPS QIPKRASFI ETGFVETLN PQFTPGQVI TTTPTPITS VDEKINVIQ LQQEIENRN QQIRDLGEK LETLKIKRQ EDKEKLKEL DKLKIQLEQ LVEFKTKIM EVQASLQRE VQRAKQEAK EAVEAKEAH AEEVADLAE AVEMATLDK EMAEEKAET LQLELEVCK EKLEEVTLD LKILKTEMQ EPGGTSASG EEAVSTYEV KQLQQQNAR LRETLVRLR DLSAHDKHE YQKLLKDID QKKSEIVEL GKTKEKLSA RVEEMEQQV ADLQEQVDA AMGAEEMVV ILGEQKLTL EEKVAQLLE EVAELEALQ DMNDQLVES NAELEAELR EDLDMARMA AKQAMRDRD AALETIADR EATLNKFRE LVQKLQEQS LQLQSRLET ESSKPVSAL PEIVDFTKM FNETKAHTK AIDLELRRV EIQQLQSHI KFLTSYMPD SFMNRGGDY DAVLVLLLV LRMIQKTEI LIGQIKDKF PGVDKIDRA AILKGHAVE QFSFRCRAS FYIYALQSI LHQYYYALN TCKTEALLK VGATYPEMA SYEKIIDGF IELVKRDQL DENVPTEAL EKCVGYFNT LFPVLLGPE NRLNHTQLL VDNVKSLTS ACDGFNNDS IVIRNLIET ANVGDIGLL AQHVITTSE QLQQQLKLI KRRLPPDSN VANIGLNKE VFENLYQCY QHCGRIIKT LQDIVKNSV QLMTSSGEL EKGLPQDKI KEIALNASD KIYEQDDLG PVQSIKNSL SFVVTQTTQ IAQFLQDNE YEIATANKN EEKPIPPIQ IRADAVKKE LEQTKTLTS KLENKESDI KELRKALKE KQEQLSEMT IRKELAEKK LGNVNKDYE LTIEKLQRK LEEAHNNYK KKEKEFEET LDHLQTDID SLENEKGEM KEKLKLLSK KTQMELSTS RSASGSQLS AQQSLGPSL PAVVKDSPL LLQEIESLR KLFHQERNE RIKLENEKI KKELDSLAP LPSFKTTPD EVLDKLFKE GALLKQEIL MSLAKPKFP QIYKVKPGN GPAAWKKHF AEENDKLAD LNRRAREFQ TKVANEIIK RKMGGRVEA DFAVFPTIE MVKALSESK PVNIGYVKI PKSYLPKGE KPRIVDLEL DFINLQKIL QSLSC
- >TC012455 RB
MSEQLKVGQ KVKVTGKDV QGVIAFIGP TSFAADTWI GLKLDEPKG KNNGSVQGV EYFKCEDKH GLFVKPSQV IPLDEHGKP LRDAGEETP KARARPSSV GTKPKPTTS RLSLASSRQ SLADSRQSL SGSRQSLAG SRQSLMGSR SHLASPIVE KSDAGDHPS QIPKRASFI ETGFVETLN PQFTPGQVI TTTPTPITS VDEKINVIQ LQQEIENRN QQIRDLGEK LETLKIKRQ EDKEKLKEL DKLKIQLEQ LVEFKTKIM EVQASLQRE VQRAKQEAK EAVEAKEAH AEEVADLAE AVEMATLDK EMAEEKAET LQLELEVCK EKLEEVTLD LKILKTEMQ EPGGTSASG EEAVSTYEV KQLQQQNAR LRETLVRLR DLSAHDKHE YQKLLKDID QKKSEIVEL GKTKEKLSA RVEEMEQQV ADLQEQVDA AMGAEEMVV ILGEQKLTL EEKVAQLLE EVAELEALQ DMNDQLVES NAELEAELR EDLDMARMA AKQAMRDRD AALETIADR EATLNKFRE LVQKLQEQS LQLQSRLET ESSKPVSAL PEIVDFTKM FNETKAHTK AIDLELRRV EIQQLQSHI KFLTSYMPD SFMNRGGDY DAVLVLLLV LRMIQKTEI LIGQIKDKF PGVDKIDRA AILKGHAVE QFSFRCRAS FYIYALQSI LHQYYYALN TCKTEALLK VGATYPEMA SYEKIIDGF IELVKRDQL DENVPTEAL EKCVGYFNT LFPVLLGPE NRLNHTQLL VDNVKSLTS ACDGFNNDS IVIRNLIET ANVGDIGLL AQHVITTSE QLQQQLKLI KRRLPPDSN VANIGLNKE VFENLYQCY QHCGRIIKT LQDIVKNSV QLMTSSGEL EKGLPQDKI KEIALNASD KIYEQDDLG PVQSIKNSL SFVVTQTTQ IAQFLQDNE YEIATANKN EEKPIPPIQ IRADAVKKE LEQTKTLTS KLENKESDI KELRKALKE KQEQLSEMT IRKELAEKK LGNVNKDYE LTIEKLQRK LEEAHNNYK KKEKEFEET LDHLQTDID SLENEKGEM KEKLKLLSK KTQMELSTS RSASGSQLS AQQSLGPSL PAVVKDSPL LLQEIESLR KLFHQERNE RIKLENEKI KKELDSLAP LPSFKTTPD EVLDKLFKE GALLKQEIL MSLAKPKFP QIYKVKPGN GPAAWKKHF AEENDKLAD LNRRAREFQ TKVANEIIK RKMGGRVEA DFAVFPTIE MVKALSESK PVNIGYVKI PKSYLPKGE KPRIVDLEL DFINLQKIL QSLSC
- >TC012455 RA
atgtcggag caactgaaa gtcggtcag aaagtcaaa gtgaccggt aaagacgtc cagggggtt attgcgttc atcggcccc acgagcttt gcggccgac acatggatc ggtctcaag ctggacgag cccaagggt aagaacaac gggagtgtt cagggggtt gaatacttc aagtgcgag gacaagcac gggctgttt gtgaagccg tcgcaggtg atccccctc gacgagcat ggcaagccg ctccgggac gccggagag gagaccccc aaggctcgg gccaggcct agcagttct cgcttgtca ctggccagt agccggcag tctttggcc gatagccgc cagtcactg tccggcagc cgccaatcg cttgcagga agtcgccaa tcccttatg gggagtcgt agtcacttg gcctcaccg attgttgaa aagtcagat gcgggggat cacccgagc caaatcccc aaacgggcc tcatttatt gagaccggc tttgtggaa accctcaat ccacaattc acaccaggc caagtgatc acgaccaca ccgactcca attacctcc gttgacgag aagattaac gtaatccag ctgcaacaa gagatcgaa aatcgcaac caacaaata cgcgatctg ggcgagaag ttggaaact ttgaaaatt aagcgccag gaggacaag gagaagttg aaggagttg gataaattg aaaatccag ttggagcaa ttggttgaa ttcaagacg aaaattatg gaagtgcag gcgagtttg cagagggag gtgcaaagg gccaagcag gaggcgaaa gaagcggtc gaggccaag gaggcgcac gctgaggaa gtggccgac ttggccgaa gccgtcgaa atggccacc cttgacaag gaaatggcg gaggaaaag gcagaaacc ttgcagctg gaattggaa gtgtgcaaa gagaagttg gaagaggtg acgctcgac ctgaaaatt ctcaaaacg gagatgcaa gaacctggt ggaacttca gcaagtggg gaagaagcc gtttccacc tacgaagtg aaacaactc cagcagcaa aatgcccga cttcgggaa actctagtc aggttgagg gatctctca gctcacgac aaacacgaa tatcagaaa cttctaaaa gacatcgac cagaagaaa tccgaaatt gtcgagttg gggaaaacg aaggaaaag ttgagtgcc agggttgaa gaaatggag caacaagtt gccgatctt caagaacag gtcgatgcg gcgatgggc gccgaagaa atggtcgta atcctcggc gagcagaag ctcaccctg gaggagaaa gtggcccaa ctcctcgaa gaagtcgcc gaactggaa gccctccaa gacatgaac gaccaactg gtcgaaagc aacgccgag ctggaagcc gaactgcgc gaagacctc gacatggcc cgaatggcc gccaaacag gcgatgcgc gaccgcgac gccgccttg gaaacaata gccgaccgc gaagccact ctcaacaaa ttccgcgaa ctcgtccaa aaactccaa gagcagtcg ctgcagctg caaagtcgg ctcgaaacc gagtcgagc aaacccgtc tcagctctg cccgaaatc gtcgacttc acgaaaatg ttcaacgag acgaaggcc cacaccaaa gctatcgac ttggagctg agacgcgtt gaaatccaa cagctgcaa agccacatc aagttcttg acctcttac atgccggat tcgttcatg aaccgcggc ggggattac gacgctgtg ctggtgctg ctgctggtc ttgcggatg attcagaag acggagatt ctgatcggg cagatcaag gacaagttt ccgggggtt gataagatt gatagggcg gcgattttg aaagggcat gctgtggag cagttctcg ttcaggtgc agggcttcg ttctatatt tatgcccta cagtcgatt ttgcaccag tattactat gcgttaaat acgtgtaaa acagaggca cttctcaaa gtcggagct acgtatcct gagatggct tcctacgag aaaatcatt gatgggttc attgaattg gtgaaacgc gatcaactg gacgagaat gtaccgact gaagcactc gaaaaatgt gttggatat ttcaataca ctgtttcct gtgcttcta ggaccggaa aatcgcctc aatcacacg caattgttg gtcgacaat gttaaaagt ttaacgtcg gcgtgtgac ggctttaat aacgattct atcgtcatc agaaacttg attgagact gccaatgtt ggtgacatc ggattgtta gcccaacac gtcataaca acatctgaa cagttgcaa caacaacta aaactgata aaacggcgc cttccgccc gactcgaac gttgccaac atcggttta aacaaggaa gtttttgag aacttgtac cagtgttac caacactgc ggccgcatc ataaaaaca ttgcaagac atcgtcaag aactcagtc cagttgatg acatctagc ggagaactg gaaaaagga ctcccacag gacaaaatc aaagaaatc gcgttgaat gccagcgat aagatctac gaacaggac gacttaggt ccagtgcaa agtattaaa aattcgctg agtttcgta gtcactcag acgacgcaa attgcgcag tttttgcaa gataacgag tacgaaatt gcgacggct aataaaaac gaggagaag cccatacct ccgatccaa attcgggcc gatgctgtg aaaaaagag ctcgaacaa acgaaaact ttgacgagc aaattggag aacaaagag tccgatatt aaagagttg cgtaaagca ctcaaagag aaacaagag caattatcc gaaatgacc atacggaag gaattagct gagaaaaag ttgggtaac gtcaataaa gattacgag ttgacgatt gaaaagctg caaaggaag ctggaagag gcgcacaat aactacaaa aagaaggag aaggagttc gaggagact ttagaccat ctccagacc gatatcgat tcgctcgag aatgaaaaa ggcgaaatg aaggagaag ttaaaactg ctctcgaag aaaacacaa atggagttg tcaacttca agaagcgcg tccggttcc caattatcc gcccaacaa tctctaggt ccaagtctc ccagctgtg gtcaaagac tcgcccttg ctcctgcaa gaaattgag agtctccgg aagctgttt caccaagaa cgcaacgag agaatcaaa ctcgaaaat gagaaaatc aaaaaggag ttggacagt ttggctccc ttgccctcg ttcaagacc acaccggac gaagttctg gataaatta ttcaaagag ggggcgtta cttaaacag gaaattttg atgagtttg gctaaaccg aagttcccg caaatttac aaagtcaag ccggggaat gggccagct gcttggaag aaacatttc gctgaggaa aacgacaag ttggcggat ctcaacagg cgagcgcgc gagttccag acgaaggta gccaatgag attatcaag cggaagatg ggggggagg gtagaagcg gatttcgca gtttttccc acgattgag atggttaag gcattgagt gagagtaag ccggtgaat atcggctac gtgaaaatc ccgaagagc tacttgccg aagggggag aagcctcgg atcgttgac ttggagttg gactttatc aacttgcag aagatcctg cagtcgcta tcgtgttaa
- >TC012455 RB
atgtcggag caactgaaa gtcggtcag aaagtcaaa gtgaccggt aaagacgtc cagggggtt attgcgttc atcggcccc acgagcttt gcggccgac acatggatc ggtctcaag ctggacgag cccaagggt aagaacaac gggagtgtt cagggggtt gaatacttc aagtgcgag gacaagcac gggctgttt gtgaagccg tcgcaggtg atccccctc gacgagcat ggcaagccg ctccgggac gccggagag gagaccccc aaggctcgg gccaggcct agcagcgtc ggtacaaaa cccaaacct acaacttct cgcttgtca ctggccagt agccggcag tctttggcc gatagccgc cagtcactg tccggcagc cgccaatcg cttgcagga agtcgccaa tcccttatg gggagtcgt agtcacttg gcctcaccg attgttgaa aagtcagat gcgggggat cacccgagc caaatcccc aaacgggcc tcatttatt gagaccggc tttgtggaa accctcaat ccacaattc acaccaggc caagtgatc acgaccaca ccgactcca attacctcc gttgacgag aagattaac gtaatccag ctgcaacaa gagatcgaa aatcgcaac caacaaata cgcgatctg ggcgagaag ttggaaact ttgaaaatt aagcgccag gaggacaag gagaagttg aaggagttg gataaattg aaaatccag ttggagcaa ttggttgaa ttcaagacg aaaattatg gaagtgcag gcgagtttg cagagggag gtgcaaagg gccaagcag gaggcgaaa gaagcggtc gaggccaag gaggcgcac gctgaggaa gtggccgac ttggccgaa gccgtcgaa atggccacc cttgacaag gaaatggcg gaggaaaag gcagaaacc ttgcagctg gaattggaa gtgtgcaaa gagaagttg gaagaggtg acgctcgac ctgaaaatt ctcaaaacg gagatgcaa gaacctggt ggaacttca gcaagtggg gaagaagcc gtttccacc tacgaagtg aaacaactc cagcagcaa aatgcccga cttcgggaa actctagtc aggttgagg gatctctca gctcacgac aaacacgaa tatcagaaa cttctaaaa gacatcgac cagaagaaa tccgaaatt gtcgagttg gggaaaacg aaggaaaag ttgagtgcc agggttgaa gaaatggag caacaagtt gccgatctt caagaacag gtcgatgcg gcgatgggc gccgaagaa atggtcgta atcctcggc gagcagaag ctcaccctg gaggagaaa gtggcccaa ctcctcgaa gaagtcgcc gaactggaa gccctccaa gacatgaac gaccaactg gtcgaaagc aacgccgag ctggaagcc gaactgcgc gaagacctc gacatggcc cgaatggcc gccaaacag gcgatgcgc gaccgcgac gccgccttg gaaacaata gccgaccgc gaagccact ctcaacaaa ttccgcgaa ctcgtccaa aaactccaa gagcagtcg ctgcagctg caaagtcgg ctcgaaacc gagtcgagc aaacccgtc tcagctctg cccgaaatc gtcgacttc acgaaaatg ttcaacgag acgaaggcc cacaccaaa gctatcgac ttggagctg agacgcgtt gaaatccaa cagctgcaa agccacatc aagttcttg acctcttac atgccggat tcgttcatg aaccgcggc ggggattac gacgctgtg ctggtgctg ctgctggtc ttgcggatg attcagaag acggagatt ctgatcggg cagatcaag gacaagttt ccgggggtt gataagatt gatagggcg gcgattttg aaagggcat gctgtggag cagttctcg ttcaggtgc agggcttcg ttctatatt tatgcccta cagtcgatt ttgcaccag tattactat gcgttaaat acgtgtaaa acagaggca cttctcaaa gtcggagct acgtatcct gagatggct tcctacgag aaaatcatt gatgggttc attgaattg gtgaaacgc gatcaactg gacgagaat gtaccgact gaagcactc gaaaaatgt gttggatat ttcaataca ctgtttcct gtgcttcta ggaccggaa aatcgcctc aatcacacg caattgttg gtcgacaat gttaaaagt ttaacgtcg gcgtgtgac ggctttaat aacgattct atcgtcatc agaaacttg attgagact gccaatgtt ggtgacatc ggattgtta gcccaacac gtcataaca acatctgaa cagttgcaa caacaacta aaactgata aaacggcgc cttccgccc gactcgaac gttgccaac atcggttta aacaaggaa gtttttgag aacttgtac cagtgttac caacactgc ggccgcatc ataaaaaca ttgcaagac atcgtcaag aactcagtc cagttgatg acatctagc ggagaactg gaaaaagga ctcccacag gacaaaatc aaagaaatc gcgttgaat gccagcgat aagatctac gaacaggac gacttaggt ccagtgcaa agtattaaa aattcgctg agtttcgta gtcactcag acgacgcaa attgcgcag tttttgcaa gataacgag tacgaaatt gcgacggct aataaaaac gaggagaag cccatacct ccgatccaa attcgggcc gatgctgtg aaaaaagag ctcgaacaa acgaaaact ttgacgagc aaattggag aacaaagag tccgatatt aaagagttg cgtaaagca ctcaaagag aaacaagag caattatcc gaaatgacc atacggaag gaattagct gagaaaaag ttgggtaac gtcaataaa gattacgag ttgacgatt gaaaagctg caaaggaag ctggaagag gcgcacaat aactacaaa aagaaggag aaggagttc gaggagact ttagaccat ctccagacc gatatcgat tcgctcgag aatgaaaaa ggcgaaatg aaggagaag ttaaaactg ctctcgaag aaaacacaa atggagttg tcaacttca agaagcgcg tccggttcc caattatcc gcccaacaa tctctaggt ccaagtctc ccagctgtg gtcaaagac tcgcccttg ctcctgcaa gaaattgag agtctccgg aagctgttt caccaagaa cgcaacgag agaatcaaa ctcgaaaat gagaaaatc aaaaaggag ttggacagt ttggctccc ttgccctcg ttcaagacc acaccggac gaagttctg gataaatta ttcaaagag ggggcgtta cttaaacag gaaattttg atgagtttg gctaaaccg aagttcccg caaatttac aaagtcaag ccggggaat gggccagct gcttggaag aaacatttc gctgaggaa aacgacaag ttggcggat ctcaacagg cgagcgcgc gagttccag acgaaggta gccaatgag attatcaag cggaagatg ggggggagg gtagaagcg gatttcgca gtttttccc acgattgag atggttaag gcattgagt gagagtaag ccggtgaat atcggctac gtgaaaatc ccgaagagc tacttgccg aagggggag aagcctcgg atcgttgac ttggagttg gactttatc aacttgcag aagatcctg cagtcgcta tcgtgttaa
- >TC012455 RA
tcaaaagag tgccaagtt cctggttgt cttgtattt tcctcgctt tatttaata attttactg gcataaaat aacataaac cctttattg gaatagtaa ataatgtaa tttagctaa atcgggtca gtttgtgca aaatgtcgg agcaactga aagtcggtc agaaagtca aagtgaccg gtaaagacg tccaggggg ttattgcgt tcatcggcc ccacgagct ttgcggccg acacatgga tcggtctca agctggacg agcccaagg gtaagaaca acgggagtg ttcaggggg ttgaatact tcaagtgcg aggacaagc acgggctgt ttgtgaagc cgtcgcagg tgatccccc tcgacgagc atggcaagc cgctccggg acgccggag aggagaccc ccaaggctc gggccaggc ctagcagtt ctcgcttgt cactggcca gtagccggc agtctttgg ccgatagcc gccagtcac tgtccggca gccgccaat cgcttgcag gaagtcgcc aatccctta tggggagtc gtagtcact tggcctcac cgattgttg aaaagtcag atgcggggg atcacccga gccaaatcc ccaaacggg cctcattta ttgagaccg gctttgtgg aaaccctca atccacaat tcacaccag gccaagtga tcacgacca caccgactc caattacct ccgttgacg agaagatta acgtaatcc agctgcaac aagagatcg aaaatcgca accaacaaa tacgcgatc tgggcgaga agttggaaa ctttgaaaa ttaagcgcc aggaggaca aggagaagt tgaaggagt tggataaat tgaaaatcc agttggagc aattggttg aattcaaga cgaaaatta tggaagtgc aggcgagtt tgcagaggg aggtgcaaa gggccaagc aggaggcga aagaagcgg tcgaggcca aggaggcgc acgctgagg aagtggccg acttggccg aagccgtcg aaatggcca cccttgaca aggaaatgg cggaggaaa aggcagaaa ccttgcagc tggaattgg aagtgtgca aagagaagt tggaagagg tgacgctcg acctgaaaa ttctcaaaa cggagatgc aagaacctg gtggaactt cagcaagtg gggaagaag ccgtttcca cctacgaag tgaaacaac tccagcagc aaaatgccc gacttcggg aaactctag tcaggttga gggatctct cagctcacg acaaacacg aatatcaga aacttctaa aagacatcg accagaaga aatccgaaa ttgtcgagt tggggaaaa cgaaggaaa agttgagtg ccagggttg aagaaatgg agcaacaag ttgccgatc ttcaagaac aggtcgatg cggcgatgg gcgccgaag aaatggtcg taatcctcg gcgagcaga agctcaccc tggaggaga aagtggccc aactcctcg aagaagtcg ccgaactgg aagccctcc aagacatga acgaccaac tggtcgaaa gcaacgccg agctggaag ccgaactgc gcgaagacc tcgacatgg cccgaatgg ccgccaaac aggcgatgc gcgaccgcg acgccgcct tggaaacaa tagccgacc gcgaagcca ctctcaaca aattccgcg aactcgtcc aaaaactcc aagagcagt cgctgcagc tgcaaagtc ggctcgaaa ccgagtcga gcaaacccg tctcagctc tgcccgaaa tcgtcgact tcacgaaaa tgttcaacg agacgaagg cccacacca aagctatcg acttggagc tgagacgcg ttgaaatcc aacagctgc aaagccaca tcaagttct tgacctctt acatgccgg attcgttca tgaaccgcg gcggggatt acgacgctg tgctggtgc tgctgctgg tcttgcgga tgattcaga agacggaga ttctgatcg ggcagatca aggacaagt ttccggggg ttgataaga ttgataggg cggcgattt tgaaagggc atgctgtgg agcagttct cgttcaggt gcagggctt cgttctata tttatgccc tacagtcga ttttgcacc agtattact atgcgttaa atacgtgta aaacagagg cacttctca aagtcggag ctacgtatc ctgagatgg cttcctacg agaaaatca ttgatgggt tcattgaat tggtgaaac gcgatcaac tggacgaga atgtaccga ctgaagcac tcgaaaaat gtgttggat atttcaata cactgtttc ctgtgcttc taggaccgg aaaatcgcc tcaatcaca cgcaattgt tggtcgaca atgttaaaa gtttaacgt cggcgtgtg acggcttta ataacgatt ctatcgtca tcagaaact tgattgaga ctgccaatg ttggtgaca tcggattgt tagcccaac acgtcataa caacatctg aacagttgc aacaacaac taaaactga taaaacggc gccttccgc ccgactcga acgttgcca acatcggtt taaacaagg aagtttttg agaacttgt accagtgtt accaacact gcggccgca tcataaaaa cattgcaag acatcgtca agaactcag tccagttga tgacatcta gcggagaac tggaaaaag gactcccac aggacaaaa tcaaagaaa tcgcgttga atgccagcg ataagatct acgaacagg acgacttag gtccagtgc aaagtatta aaaattcgc tgagtttcg tagtcactc agacgacgc aaattgcgc agtttttgc aagataacg agtacgaaa ttgcgacgg ctaataaaa acgaggaga agcccatac ctccgatcc aaattcggg ccgatgctg tgaaaaaag agctcgaac aaacgaaaa ctttgacga gcaaattgg agaacaaag agtccgata ttaaagagt tgcgtaaag cactcaaag agaaacaag agcaattat ccgaaatga ccatacgga aggaattag ctgagaaaa agttgggta acgtcaata aagattacg agttgacga ttgaaaagc tgcaaagga agctggaag aggcgcaca ataactaca aaaagaagg agaaggagt tcgaggaga ctttagacc atctccaga ccgatatcg attcgctcg agaatgaaa aaggcgaaa tgaaggaga agttaaaac tgctctcga agaaaacac aaatggagt tgtcaactt caagaagcg cgtccggtt cccaattat ccgcccaac aatctctag gtccaagtc tcccagctg tggtcaaag actcgccct tgctcctgc aagaaattg agagtctcc ggaagctgt ttcaccaag aacgcaacg agagaatca aactcgaaa atgagaaaa tcaaaaagg agttggaca gtttggctc ccttgccct cgttcaaga ccacaccgg acgaagttc tggataaat tattcaaag agggggcgt tacttaaac aggaaattt tgatgagtt tggctaaac cgaagttcc cgcaaattt acaaagtca agccgggga atgggccag ctgcttgga agaaacatt tcgctgagg aaaacgaca agttggcgg atctcaaca ggcgagcgc gcgagttcc agacgaagg tagccaatg agattatca agcggaaga tggggggga gggtagaag cggatttcg cagtttttc ccacgattg agatggtta aggcattga gtgagagta agccggtga atatcggct acgtgaaaa tcccgaaga gctacttgc cgaaggggg agaagcctc ggatcgttg acttggagt tggacttta tcaacttgc agaagatcc tgcagtcgc tatcgtgtt aacttattt tctataact gtaataaat tgaattaat tggt
- >TC012455 RB
tcaaaagag tgccaagtt cctggttgt cttgtattt tcctcgctt tatttaata attttactg gcataaaat aacataaac cctttattg gaatagtaa ataatgtaa tttagctaa atcgggtca gtttgtgca aaatgtcgg agcaactga aagtcggtc agaaagtca aagtgaccg gtaaagacg tccaggggg ttattgcgt tcatcggcc ccacgagct ttgcggccg acacatgga tcggtctca agctggacg agcccaagg gtaagaaca acgggagtg ttcaggggg ttgaatact tcaagtgcg aggacaagc acgggctgt ttgtgaagc cgtcgcagg tgatccccc tcgacgagc atggcaagc cgctccggg acgccggag aggagaccc ccaaggctc gggccaggc ctagcagcg tcggtacaa aacccaaac ctacaactt ctcgcttgt cactggcca gtagccggc agtctttgg ccgatagcc gccagtcac tgtccggca gccgccaat cgcttgcag gaagtcgcc aatccctta tggggagtc gtagtcact tggcctcac cgattgttg aaaagtcag atgcggggg atcacccga gccaaatcc ccaaacggg cctcattta ttgagaccg gctttgtgg aaaccctca atccacaat tcacaccag gccaagtga tcacgacca caccgactc caattacct ccgttgacg agaagatta acgtaatcc agctgcaac aagagatcg aaaatcgca accaacaaa tacgcgatc tgggcgaga agttggaaa ctttgaaaa ttaagcgcc aggaggaca aggagaagt tgaaggagt tggataaat tgaaaatcc agttggagc aattggttg aattcaaga cgaaaatta tggaagtgc aggcgagtt tgcagaggg aggtgcaaa gggccaagc aggaggcga aagaagcgg tcgaggcca aggaggcgc acgctgagg aagtggccg acttggccg aagccgtcg aaatggcca cccttgaca aggaaatgg cggaggaaa aggcagaaa ccttgcagc tggaattgg aagtgtgca aagagaagt tggaagagg tgacgctcg acctgaaaa ttctcaaaa cggagatgc aagaacctg gtggaactt cagcaagtg gggaagaag ccgtttcca cctacgaag tgaaacaac tccagcagc aaaatgccc gacttcggg aaactctag tcaggttga gggatctct cagctcacg acaaacacg aatatcaga aacttctaa aagacatcg accagaaga aatccgaaa ttgtcgagt tggggaaaa cgaaggaaa agttgagtg ccagggttg aagaaatgg agcaacaag ttgccgatc ttcaagaac aggtcgatg cggcgatgg gcgccgaag aaatggtcg taatcctcg gcgagcaga agctcaccc tggaggaga aagtggccc aactcctcg aagaagtcg ccgaactgg aagccctcc aagacatga acgaccaac tggtcgaaa gcaacgccg agctggaag ccgaactgc gcgaagacc tcgacatgg cccgaatgg ccgccaaac aggcgatgc gcgaccgcg acgccgcct tggaaacaa tagccgacc gcgaagcca ctctcaaca aattccgcg aactcgtcc aaaaactcc aagagcagt cgctgcagc tgcaaagtc ggctcgaaa ccgagtcga gcaaacccg tctcagctc tgcccgaaa tcgtcgact tcacgaaaa tgttcaacg agacgaagg cccacacca aagctatcg acttggagc tgagacgcg ttgaaatcc aacagctgc aaagccaca tcaagttct tgacctctt acatgccgg attcgttca tgaaccgcg gcggggatt acgacgctg tgctggtgc tgctgctgg tcttgcgga tgattcaga agacggaga ttctgatcg ggcagatca aggacaagt ttccggggg ttgataaga ttgataggg cggcgattt tgaaagggc atgctgtgg agcagttct cgttcaggt gcagggctt cgttctata tttatgccc tacagtcga ttttgcacc agtattact atgcgttaa atacgtgta aaacagagg cacttctca aagtcggag ctacgtatc ctgagatgg cttcctacg agaaaatca ttgatgggt tcattgaat tggtgaaac gcgatcaac tggacgaga atgtaccga ctgaagcac tcgaaaaat gtgttggat atttcaata cactgtttc ctgtgcttc taggaccgg aaaatcgcc tcaatcaca cgcaattgt tggtcgaca atgttaaaa gtttaacgt cggcgtgtg acggcttta ataacgatt ctatcgtca tcagaaact tgattgaga ctgccaatg ttggtgaca tcggattgt tagcccaac acgtcataa caacatctg aacagttgc aacaacaac taaaactga taaaacggc gccttccgc ccgactcga acgttgcca acatcggtt taaacaagg aagtttttg agaacttgt accagtgtt accaacact gcggccgca tcataaaaa cattgcaag acatcgtca agaactcag tccagttga tgacatcta gcggagaac tggaaaaag gactcccac aggacaaaa tcaaagaaa tcgcgttga atgccagcg ataagatct acgaacagg acgacttag gtccagtgc aaagtatta aaaattcgc tgagtttcg tagtcactc agacgacgc aaattgcgc agtttttgc aagataacg agtacgaaa ttgcgacgg ctaataaaa acgaggaga agcccatac ctccgatcc aaattcggg ccgatgctg tgaaaaaag agctcgaac aaacgaaaa ctttgacga gcaaattgg agaacaaag agtccgata ttaaagagt tgcgtaaag cactcaaag agaaacaag agcaattat ccgaaatga ccatacgga aggaattag ctgagaaaa agttgggta acgtcaata aagattacg agttgacga ttgaaaagc tgcaaagga agctggaag aggcgcaca ataactaca aaaagaagg agaaggagt tcgaggaga ctttagacc atctccaga ccgatatcg attcgctcg agaatgaaa aaggcgaaa tgaaggaga agttaaaac tgctctcga agaaaacac aaatggagt tgtcaactt caagaagcg cgtccggtt cccaattat ccgcccaac aatctctag gtccaagtc tcccagctg tggtcaaag actcgccct tgctcctgc aagaaattg agagtctcc ggaagctgt ttcaccaag aacgcaacg agagaatca aactcgaaa atgagaaaa tcaaaaagg agttggaca gtttggctc ccttgccct cgttcaaga ccacaccgg acgaagttc tggataaat tattcaaag agggggcgt tacttaaac aggaaattt tgatgagtt tggctaaac cgaagttcc cgcaaattt acaaagtca agccgggga atgggccag ctgcttgga agaaacatt tcgctgagg aaaacgaca agttggcgg atctcaaca ggcgagcgc gcgagttcc agacgaagg tagccaatg agattatca agcggaaga tggggggga gggtagaag cggatttcg cagtttttc ccacgattg agatggtta aggcattga gtgagagta agccggtga atatcggct acgtgaaaa tcccgaaga gctacttgc cgaaggggg agaagcctc ggatcgttg acttggagt tggacttta tcaacttgc agaagatcc tgcagtcgc tatcgtgtt aacttattt tctataact gtaataaat tgaattaat tggt
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