Status
Please wait ...
Gene information
General information
TC034340
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012026,TC012025,TC012024
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid66: 4793797..4813463
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034340
    iBeetle screen iB_02920
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    posterior terminus sometimes remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    gut sometimes remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    appendages sometimes partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    head partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Mandibles and labium seem to be the most often affected head parts.
    mandible not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    labium not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034340 RA
      MDSSSHTTT IVKVYKYRW MTLLIFCFY SSINFVQFL 
      QYSIISNIT VKYYNVEEI LVDLTGLFF MISFIIFCL 
      PVNHLIEKY NLRITALIT SGLTTFGNL IKLLSVSPN 
      RFYVALIGQ ALCSIAQVF AMSIPSKLA STWFGPEEV 
      STACAIAIL GTQIGVAIG CIMPTHLVR NSQNLDEIG 
      DDFMTLHII NGSITVTTF IVVLLARPP QPPSQSQVG 
      IIEAEKPST MSIIKQMVR NRDYVLLVI AIGSSNGLY 
      SSFGILFNG LYLNYFPVI ILTVFYFEK TGYESEAGI 
      ISALAVVAG GCIGSVIFG YVLDKWHHF KLTTFSIII 
      SSAVTYIFV IVSMEEKAQ LATYFTIPL FGFFIASTL 
      LVGFEFATE LMYPVPEAA SYKMCNRVD CLSNATTVK 
      VYKYRWIIL LIFCFFSSI NFLQFFQYS IISNITVKY 
      YDVEDTLVD FTGLIFMIS FILLCLPAN QFIEKYNLR 
      NTALLSSGL TALGNLVKL FSISRDRFY VVIIGQVLC 
      AIAQVFLTS VPSKLASTW FGPDEVSTA CAVAIFGSH 
      IGLAIGCFM PSSLVRNSD NLDEIGDDF MTLQIINGS 
      ITVASFIAV LLFFRAKPP LPPSQSQVA IIDAAKPSI 
      ISIIKQMIN NRDYLLVVV VIGSSNGLY SSFGILFNS 
      VYLHYFPGS ETAVGIIAA LTVVAGGCI GSIIFGYVL 
      DKWHHFKLT TFFIVFFAA VTFSLQIVL MEAKAQLAT 
      YFTIPLFGI VMDSLSNTS AVKVYKYRW ITLLIFCFY 
      SAINFLQFF QYSIISNIT VKYYDVEDT LVDLTGLVF 
      MISFILLCL PANQFIEKY VTDPLHDSQ QRSNPIFPE 
      FEKHSPVIQ RLNNPRKPN KIILNISRS ILCRDNWPS 
      PLCHCPSFH DKCSIEISL DLGYETAVG LIAALAVVA 
      GGCIGSVIF GYVLDKWHH FKYFPQTVT HSYNFISRL 
      TTFFIVFFS AVTFGFEVV SLEARAQLA TYFTAPLFG
    CDS sequence
    • >TC034340 RA
      atggattcc tcgtcacat actacaaca atagtgaaa 
      gtgtacaaa tatcgatgg atgactctt ttaatattt 
      tgcttctat tcttcaata aacttcgtg cagtttctt 
      caatattcg atcatttcg aacatcaca gtcaagtac 
      tacaatgtg gaagaaata cttgtggac ctcactggt 
      ctattcttc atgatttcc tttataata ttttgcctt 
      ccggtcaac caccttatc gaaaaatac aatttacga 
      atcacagct cttataact agcggccta acaacgttc 
      ggaaactta ataaaattg ctctcagta tctccgaac 
      cgcttttat gtcgcttta atcggccaa gctctctgt 
      tcgattgcc caagttttt gcgatgagt atcccgtcg 
      aaattagca tcgacttgg tttggacct gaagaggtg 
      tccacagct tgcgccatc gcaattttg gggacgcaa 
      atcggggtt gcgatcggt tgtattatg cccacacat 
      ttggtgagg aacagccag aatcttgac gaaatcggt 
      gatgatttt atgacgctg catataatc aacggctct 
      ataaccgtg accactttt atcgtagtt ttgctggct 
      agaccgcca cagccccca agccagtct caagtgggt 
      attattgaa gctgagaaa ccttcgact atgtcgatt 
      atcaagcaa atggtcagg aatcgagat tatgtgttg 
      ttggtcatt gcgataggg agctcgaat gggctgtat 
      tcgtcgttt gggattttg ttcaacgga ctctacctc 
      aattatttt cctgtaatt atacttaca gttttctat 
      tttgaaaaa acaggttat gagtccgaa gctgggata 
      atttcagct ttggccgta gttgctggt gggtgtatc 
      ggcagtgta atttttgga tacgtttta gacaagtgg 
      caccacttc aaactcacc accttttcc attattatt 
      tcttctgca gtcacgtac atttttgta attgtctca 
      atggaagag aaggcgcag ttggccaca tatttcacc 
      attccgcta tttggattt ttcattgcg agtacatta 
      ctcgtcggt tttgagttt gcaaccgaa ctcatgtat 
      ccggttcct gaagctgcg tcttacaaa atgtgtaac 
      agagtagac tgtctatcg aatgccaca accgtgaaa 
      gtgtacaaa tatcgatgg attatcctg ttaatattt 
      tgcttcttt tcttcgata aatttcttg cagtttttc 
      caatactca atcatttcc aacatcaca gtcaaatat 
      tacgatgtg gaagacaca cttgtggac ttcacgggg 
      ctaattttc atgatttcg tttatatta ctttgcctt 
      ccagccaac caatttatc gaaaaatac aatttgaga 
      aacacagcc ctgttatcc agcggctta acagctttg 
      ggaaaccta gttaaatta ttctccata tctcgcgat 
      cgattttat gtcgtgata attggccaa gtcctctgt 
      gccattgcc caagttttc ctgacaagt gttccatcg 
      aaattagcc tcgacttgg tttgggcca gatgaagtg 
      tccacagct tgcgctgtc gcaattttt ggaagtcac 
      atcggactt gcgatcggt tgtttcatg ccttcaagc 
      ttggtcaga aatagcgac aatctggac gaaatcggt 
      gacgatttt atgacacta caaataatc aacggctct 
      ataacggtc gccagtttt attgcagtt ttgctgttt 
      ttcagggct aaacctcca ctgccgcca agtcagtct 
      caagtcgcc atcattgat gcggcgaaa ccgtcgatt 
      atatctatt atcaagcaa atgatcaac aatagagat 
      tacttgttg gtggtggtg gtgatagga agttcgaat 
      ggattgtat tcctcgttt ggaattttg tttaacagc 
      gtttacctc cactatttt cctggctct gagaccgca 
      gttggcata atcgctgcc ctcactgtt gttgctggc 
      ggatgcatc ggtagtata atttttggc tatgttttg 
      gacaagtgg caccacttt aaactcacc accttcttc 
      attgttttc tttgcagca gtgacattc agtttacag 
      attgtttta atggaagca aaggcacaa ctggccaca 
      tatttcacc ataccgtta tttggtatt gtaatggac 
      tccttatca aacacgtcg gccgtgaaa gtctacaaa 
      tatcgatgg attacactt ttaatattt tgcttctat 
      tctgcgata aatttccta cagtttttc cagtactca 
      atcatttcc aacatcaca gtcaaatat tacgatgtg 
      gaagacaca cttgtggac ctcacaggt ctagtcttc 
      atgatttcc tttatatta ctttgcctt ccagccaac 
      caatttatc gaaaaatac gtaactgac ccgttacat 
      gacagtcaa caaagatca aacccaatt tttccagaa 
      tttgagaaa cacagccct gttatccag cggcttaac 
      aaccctcgg aaacctaat aaaattatt ctcaatatc 
      tcccgatcg attttatgt cgtgataat tggccaagt 
      cctctgtgc cattgccca agttttcat gacaagtgt 
      tccatcgaa attagcctc gacttgggc tacgaaacc 
      gcagttggc ctaatagca gccctggct gttgttgct 
      ggcggatgc atcggtagt gtaattttt ggctacgtt 
      ttggacaag tggcaccac tttaagtat tttccccaa 
      acagttacc cactcatac aattttatt tctagactc 
      accactttt ttcattgtg ttcttttct gcggtgact 
      ttcggcttc gaggttgtt tcactggaa gcaagggcc 
      caattggcc acgtacttc accgcaccg ttatttggg 
      tga
    mRNA sequence
    • >TC034340 RA
      taagtataa aacaataac attttaatt tattgaagg 
      tatctacaa ttcaaaaca ttatttttt actgacaaa 
      acgcaaact tggacccca aacaataac ttactgtta 
      tcgtagtga tggtcgcac atgcggtaa caattgtat 
      ggtccagga cacaactgt ttatgtgtt cataattaa 
      catagcgac agttcatat cttatcttg cagtaccta 
      ttaaataaa actttcgat tcgttataa tcattttac 
      ctattgtaa tggttataa tcctattgg ccttgaatt 
      ttgcactat acacacaac tgctgcaat taaatacat 
      aatttttta aggtttttt tcatttctg tgtgagctg 
      tgttgtagt tcgtatcgc aagtaatga gtgcgatgt 
      gttgtttca attacaaac ttcagcact tgtgacaat 
      aatccttaa tccgacgaa acgaattcg gaatggatt 
      cctcgtcac atactacaa caatagtga aagtgtaca 
      aatatcgat ggatgactc ttttaatat tttgcttct 
      attcttcaa taaacttcg tgcagtttc ttcaatatt 
      cgatcattt cgaacatca cagtcaagt actacaatg 
      tggaagaaa tacttgtgg acctcactg gtctattct 
      tcatgattt cctttataa tattttgcc ttccggtca 
      accacctta tcgaaaaat acaatttac gaatcacag 
      ctcttataa ctagcggcc taacaacgt tcggaaact 
      taataaaat tgctctcag tatctccga accgctttt 
      atgtcgctt taatcggcc aagctctct gttcgattg 
      cccaagttt ttgcgatga gtatcccgt cgaaattag 
      catcgactt ggtttggac ctgaagagg tgtccacag 
      cttgcgcca tcgcaattt tggggacgc aaatcgggg 
      ttgcgatcg gttgtatta tgcccacac atttggtga 
      ggaacagcc agaatcttg acgaaatcg gtgatgatt 
      ttatgacgc tgcatataa tcaacggct ctataaccg 
      tgaccactt ttatcgtag ttttgctgg ctagaccgc 
      cacagcccc caagccagt ctcaagtgg gtattattg 
      aagctgaga aaccttcga ctatgtcga ttatcaagc 
      aaatggtca ggaatcgag attatgtgt tgttggtca 
      ttgcgatag ggagctcga atgggctgt attcgtcgt 
      ttgggattt tgttcaacg gactctacc tcaattatt 
      ttcctgtaa ttatactta cagttttct attttgaaa 
      aaacaggtt atgagtccg aagctggga taatttcag 
      ctttggccg tagttgctg gtgggtgta tcggcagtg 
      taatttttg gatacgttt tagacaagt ggcaccact 
      tcaaactca ccacctttt ccattatta tttcttctg 
      cagtcacgt acatttttg taattgtct caatggaag 
      agaaggcgc agttggcca catatttca ccattccgc 
      tatttggat ttttcattg cgagtacat tactcgtcg 
      gttttgagt ttgcaaccg aactcatgt atccggttc 
      ctgaagctg cgtcttaca aaatgtgta acagagtag 
      actgtctat cgaatgcca caaccgtga aagtgtaca 
      aatatcgat ggattatcc tgttaatat tttgcttct 
      tttcttcga taaatttct tgcagtttt tccaatact 
      caatcattt ccaacatca cagtcaaat attacgatg 
      tggaagaca cacttgtgg acttcacgg ggctaattt 
      tcatgattt cgtttatat tactttgcc ttccagcca 
      accaattta tcgaaaaat acaatttga gaaacacag 
      ccctgttat ccagcggct taacagctt tgggaaacc 
      tagttaaat tattctcca tatctcgcg atcgatttt 
      atgtcgtga taattggcc aagtcctct gtgccattg 
      cccaagttt tcctgacaa gtgttccat cgaaattag 
      cctcgactt ggtttgggc cagatgaag tgtccacag 
      cttgcgctg tcgcaattt ttggaagtc acatcggac 
      ttgcgatcg gttgtttca tgccttcaa gcttggtca 
      gaaatagcg acaatctgg acgaaatcg gtgacgatt 
      ttatgacac tacaaataa tcaacggct ctataacgg 
      tcgccagtt ttattgcag ttttgctgt ttttcaggg 
      ctaaacctc cactgccgc caagtcagt ctcaagtcg 
      ccatcattg atgcggcga aaccgtcga ttatatcta 
      ttatcaagc aaatgatca acaatagag attacttgt 
      tggtggtgg tggtgatag gaagttcga atggattgt 
      attcctcgt ttggaattt tgtttaaca gcgtttacc 
      tccactatt ttcctggct ctgagaccg cagttggca 
      taatcgctg ccctcactg ttgttgctg gcggatgca 
      tcggtagta taatttttg gctatgttt tggacaagt 
      ggcaccact ttaaactca ccaccttct tcattgttt 
      tctttgcag cagtgacat tcagtttac agattgttt 
      taatggaag caaaggcac aactggcca catatttca 
      ccataccgt tatttggta ttgtaatgg actccttat 
      caaacacgt cggccgtga aagtctaca aatatcgat 
      ggattacac ttttaatat tttgcttct attctgcga 
      taaatttcc tacagtttt tccagtact caatcattt 
      ccaacatca cagtcaaat attacgatg tggaagaca 
      cacttgtgg acctcacag gtctagtct tcatgattt 
      cctttatat tactttgcc ttccagcca accaattta 
      tcgaaaaat acgtaactg acccgttac atgacagtc 
      aacaaagat caaacccaa tttttccag aatttgaga 
      aacacagcc ctgttatcc agcggctta acaaccctc 
      ggaaaccta ataaaatta ttctcaata tctcccgat 
      cgattttat gtcgtgata attggccaa gtcctctgt 
      gccattgcc caagttttc atgacaagt gttccatcg 
      aaattagcc tcgacttgg gctacgaaa ccgcagttg 
      gcctaatag cagccctgg ctgttgttg ctggcggat 
      gcatcggta gtgtaattt ttggctacg ttttggaca 
      agtggcacc actttaagt attttcccc aaacagtta 
      cccactcat acaatttta tttctagac tcaccactt 
      ttttcattg tgttctttt ctgcggtga ctttcggct 
      tcgaggttg tttcactgg aagcaaggg cccaattgg 
      ccacgtact tcaccgcac cgttatttg ggtgagtca 
      aaaatcgca aaatttttc attaacaga aaatcgttg 
      cagattttt catcgggag cacattact agtcggttt 
      tgagtttgc aaccgaact catgtatcc cgtccctga 
      agcc