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Gene information
General information
TC012450 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7674010..7677834
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC012450
      iBeetle screen iB_02919

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC012450 RA
        MLNPFSSTN TNIFDERTH NTTPANLCG QNVLVSQEQ 
        HRKIFNTLT TSITGMPGT MQPSFHNPN VVTFTFKPN 
        QDYYPQNYW CCQTPAMQN LNQLQQQTA ADLLQLQRQ 
        LNQPYQCKF HLDSQTSTT DLGAKPEAR PDLKNLLCT 
        CAWLQGPIS KNHKKLILK QVTPKIEGS ICDQIDQEE 
        HKAKKVNFE CAEGTKNAE VQVECKCPT CPGQEAPAR 
        VKKKRRRSR KKSPTTTSD ESTDNESDN RNLEFVTQL 
        LYYVANSRS KLEVIYQRL MNCKMAQDT LDSLLSCIQ 
        NNQNKWEEI LQSFENKCY CNEIIDENR ACIYKLADN 
        LFATLSNHY EMLDSIESK ATDCYVKNK EEPIYLLFQ 
        IMDMNLNML DKINIHIEK LKNRVDFYK NIWAECNKR 
        CTWT
      • >TC012450 RB
        MLNPFSSTN TNIFDERTH NTTPANLCG QNVLVSQEQ 
        HRKIFNTLT TSITGMPGT MQPSFHNPN VVTFTFKPN 
        QDYYPQNYW CCQTPAMQN LNQLQQQTA ADLLQLQRQ 
        LNQPYQCKF HLDSQTSTT DLGAKPGSI CDQIDQEEH 
        KAKKVNFEC AEGTKNAEV QVECKCPTC PGQEAPARV 
        KKKRRRSRK KSPTTTSDE STDNESDNR NLEFVTQLL 
        YYVANSRSK LEVIYQRLM NCKMAQDTL DSLLSCIQN 
        NQNKWEEIL QSFENKCYC NEIIDENRA CIYKLADNL 
        FATLSNHYE MLDSIESKA TDCYVKNKE EPIYLLFQI 
        MDMNLNMLD KINIHIEKL KNRVDFYKN IWAECNKRC 
        TWT
      CDS sequence
      • >TC012450 RA
        atgttgaac ccattttca tcaaccaac accaacatc 
        ttcgacgaa cgaactcac aatacaacc ccggcaaac 
        ttgtgcggc caaaatgtc ctagtttcg caggaacag 
        catcgcaaa atcttcaac actctaaca acctccatc 
        accggaatg cccggaaca atgcaacca agtttccac 
        aaccccaac gtcgtcacc tttactttc aaaccgaac 
        caagactat tacccccaa aattactgg tgctgtcaa 
        actcccgca atgcaaaat ctgaaccag ttgcaacaa 
        caaaccgcc gccgatttg ctccagctc caaagacag 
        ctaaatcag ccctaccag tgcaagttc cacctcgat 
        agccagact tcgaccaca gatttgggg gcgaaacca 
        gaagcgagg cctgatttg aaaaacctc ctttgtact 
        tgcgcatgg ctacaaggc ccgatttcg aaaaaccac 
        aaaaaacta atcttgaag caagtaacg cctaaaatt 
        gaagggagc atctgcgac cagatcgac caagaggaa 
        cacaaggcg aagaaagtc aacttcgag tgtgccgaa 
        gggacgaaa aatgccgaa gttcaagtt gagtgcaag 
        tgtccaact tgtcccggc caggaagct ccagcacga 
        gttaagaaa aagcgaaga aggagtcgg aaaaaatca 
        ccaacgacc acaagcgac gaaagcacc gataacgag 
        agtgacaac cggaatttg gagtttgtt acacaattg 
        ctttattac gtggcaaat tcgagaagc aagttggag 
        gtgatttac cagcgctta atgaattgc aaaatggca 
        caagatact ctcgactct ttgttatcg tgcattcaa 
        aacaatcaa aataaatgg gaggaaatt ttgcaaagc 
        tttgaaaat aaatgttat tgtaacgaa atcattgat 
        gaaaatcgc gcttgcata tacaaactc gctgataat 
        ttattcgca accttgtcc aaccactac gagatgctt 
        gatagtatt gagagtaaa gcaaccgac tgttatgtc 
        aaaaacaag gaagaaccg atctattta ctattccaa 
        atcatggac atgaactta aacatgttg gataaaatt 
        aacattcac atcgagaaa ctgaagaat cgtgtggat 
        ttctacaaa aacatttgg gcggaatgc aacaaacgc 
        tgcacttgg acgtga
      • >TC012450 RB
        atgttgaac ccattttca tcaaccaac accaacatc 
        ttcgacgaa cgaactcac aatacaacc ccggcaaac 
        ttgtgcggc caaaatgtc ctagtttcg caggaacag 
        catcgcaaa atcttcaac actctaaca acctccatc 
        accggaatg cccggaaca atgcaacca agtttccac 
        aaccccaac gtcgtcacc tttactttc aaaccgaac 
        caagactat tacccccaa aattactgg tgctgtcaa 
        actcccgca atgcaaaat ctgaaccag ttgcaacaa 
        caaaccgcc gccgatttg ctccagctc caaagacag 
        ctaaatcag ccctaccag tgcaagttc cacctcgat 
        agccagact tcgaccaca gatttgggg gcgaaacca 
        gggagcatc tgcgaccag atcgaccaa gaggaacac 
        aaggcgaag aaagtcaac ttcgagtgt gccgaaggg 
        acgaaaaat gccgaagtt caagttgag tgcaagtgt 
        ccaacttgt cccggccag gaagctcca gcacgagtt 
        aagaaaaag cgaagaagg agtcggaaa aaatcacca 
        acgaccaca agcgacgaa agcaccgat aacgagagt 
        gacaaccgg aatttggag tttgttaca caattgctt 
        tattacgtg gcaaattcg agaagcaag ttggaggtg 
        atttaccag cgcttaatg aattgcaaa atggcacaa 
        gatactctc gactctttg ttatcgtgc attcaaaac 
        aatcaaaat aaatgggag gaaattttg caaagcttt 
        gaaaataaa tgttattgt aacgaaatc attgatgaa 
        aatcgcgct tgcatatac aaactcgct gataattta 
        ttcgcaacc ttgtccaac cactacgag atgcttgat 
        agtattgag agtaaagca accgactgt tatgtcaaa 
        aacaaggaa gaaccgatc tatttacta ttccaaatc 
        atggacatg aacttaaac atgttggat aaaattaac 
        attcacatc gagaaactg aagaatcgt gtggatttc 
        tacaaaaac atttgggcg gaatgcaac aaacgctgc 
        acttggacg tga
      mRNA sequence
      • >TC012450 RA
        ccgtatgtc aaaatgttg aacccattt tcatcaacc 
        aacaccaac atcttcgac gaacgaact cacaataca 
        accccggca aacttgtgc ggccaaaat gtcctagtt 
        tcgcaggaa cagcatcgc aaaatcttc aacactcta 
        acaacctcc atcaccgga atgcccgga acaatgcaa 
        ccaagtttc cacaacccc aacgtcgtc acctttact 
        ttcaaaccg aaccaagac tattacccc caaaattac 
        tggtgctgt caaactccc gcaatgcaa aatctgaac 
        cagttgcaa caacaaacc gccgccgat ttgctccag 
        ctccaaaga cagctaaat cagccctac cagtgcaag 
        ttccacctc gatagccag acttcgacc acagatttg 
        ggggcgaaa ccagaagcg aggcctgat ttgaaaaac 
        ctcctttgt acttgcgca tggctacaa ggcccgatt 
        tcgaaaaac cacaaaaaa ctaatcttg aagcaagta 
        acgcctaaa attgaaggg agcatctgc gaccagatc 
        gaccaagag gaacacaag gcgaagaaa gtcaacttc 
        gagtgtgcc gaagggacg aaaaatgcc gaagttcaa 
        gttgagtgc aagtgtcca acttgtccc ggccaggaa 
        gctccagca cgagttaag aaaaagcga agaaggagt 
        cggaaaaaa tcaccaacg accacaagc gacgaaagc 
        accgataac gagagtgac aaccggaat ttggagttt 
        gttacacaa ttgctttat tacgtggca aattcgaga 
        agcaagttg gaggtgatt taccagcgc ttaatgaat 
        tgcaaaatg gcacaagat actctcgac tctttgtta 
        tcgtgcatt caaaacaat caaaataaa tgggaggaa 
        attttgcaa agctttgaa aataaatgt tattgtaac 
        gaaatcatt gatgaaaat cgcgcttgc atatacaaa 
        ctcgctgat aatttattc gcaaccttg tccaaccac 
        tacgagatg cttgatagt attgagagt aaagcaacc 
        gactgttat gtcaaaaac aaggaagaa ccgatctat 
        ttactattc caaatcatg gacatgaac ttaaacatg 
        ttggataaa attaacatt cacatcgag aaactgaag 
        aatcgtgtg gatttctac aaaaacatt tgggcggaa 
        tgcaacaaa cgctgcact tggacgtga cttatttat 
        ttatatttg cttctcttc gccgcaaat tggacctaa 
        taaaataca caagaagtg
      • >TC012450 RB
        ccgtatgtc aaaatgttg aacccattt tcatcaacc 
        aacaccaac atcttcgac gaacgaact cacaataca 
        accccggca aacttgtgc ggccaaaat gtcctagtt 
        tcgcaggaa cagcatcgc aaaatcttc aacactcta 
        acaacctcc atcaccgga atgcccgga acaatgcaa 
        ccaagtttc cacaacccc aacgtcgtc acctttact 
        ttcaaaccg aaccaagac tattacccc caaaattac 
        tggtgctgt caaactccc gcaatgcaa aatctgaac 
        cagttgcaa caacaaacc gccgccgat ttgctccag 
        ctccaaaga cagctaaat cagccctac cagtgcaag 
        ttccacctc gatagccag acttcgacc acagatttg 
        ggggcgaaa ccagggagc atctgcgac cagatcgac 
        caagaggaa cacaaggcg aagaaagtc aacttcgag 
        tgtgccgaa gggacgaaa aatgccgaa gttcaagtt 
        gagtgcaag tgtccaact tgtcccggc caggaagct 
        ccagcacga gttaagaaa aagcgaaga aggagtcgg 
        aaaaaatca ccaacgacc acaagcgac gaaagcacc 
        gataacgag agtgacaac cggaatttg gagtttgtt 
        acacaattg ctttattac gtggcaaat tcgagaagc 
        aagttggag gtgatttac cagcgctta atgaattgc 
        aaaatggca caagatact ctcgactct ttgttatcg 
        tgcattcaa aacaatcaa aataaatgg gaggaaatt 
        ttgcaaagc tttgaaaat aaatgttat tgtaacgaa 
        atcattgat gaaaatcgc gcttgcata tacaaactc 
        gctgataat ttattcgca accttgtcc aaccactac 
        gagatgctt gatagtatt gagagtaaa gcaaccgac 
        tgttatgtc aaaaacaag gaagaaccg atctattta 
        ctattccaa atcatggac atgaactta aacatgttg 
        gataaaatt aacattcac atcgagaaa ctgaagaat 
        cgtgtggat ttctacaaa aacatttgg gcggaatgc 
        aacaaacgc tgcacttgg acgtgactt atttattta 
        tatttgctt ctcttcgcc gcaaattgg acctaataa 
        aatacacaa gaagtg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY