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Gene information
General information
TC012433 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7326980..7350984
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012433
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02918

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    tropharium structure irregular - Number of animals showing the phenotype: 4
    tropharium slightly shortened - Number of animals showing the phenotype: 4
    tropharium potentially partiallydegenerated - Number of animals showing the phenotype: 4

    vitellogenic egg chamber potentially partiallyfused - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late partiallydegenerated - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    Pupal morphology
    abdomen gin-trap setae not present - Number of animals showing the phenotype: 8

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012433 RA
      MGNQLIGIA PSQILPVER YLTEHPDLK FDLGLGSTR 
      FFKVARAES QEGLVVVKI FAIHDPTLP LSTYKDQLD 
      EIRKRLSSA VNCIPFQRI ILTEKAGIL VREFVKYSL 
      YDRISTRPF LTNIEKRWI TFQILYALH QCHKVGVCH 
      GDIKLENIT VTSWNWVLL VDFASFKPT FVPEDNPAD 
      YSYFFDTSR RRTCYIAPE RFSKTTNNE NSALITETT 
      CESGELTPA MDIFSTGCA LLELWNELH VPFEYSQLL 
      AYRSNKYSP EKHLNNLNN PSLKSLISS MIEIDPSKR 
      LSAEDYLAK ERGKLFPEY FFTFLQSYM LIFSATPIL 
      SPDEKILRL KSDIANIFK FLGPIKHSH EDDSEKYEK 
      GESEGLVII ISLVTSCIR GLHDCSSKL CSLEILLEL 
      SNHANSETI LDRILPYIM YLARDNSSR VKIAAINTI 
      TKCLELVQQ LPKSDSNIF PEYVLPGLA PLAGDPNTC 
      VRAAYAKNI ATLAEIALR YLDQMQSDW YDNNIKQKY 
      NHTFNYELE LQALHEMVE QTVSVLLTD SQALVKQTL 
      IENGITKLC VFFGKQKAN DVLLSHMIT FLNDKDDKQ 
      LRGSFFDCI VGVAAYIGW HCSGILTPL LLQGLTDPC 
      EFVTTKSIN AMSALTELG LLTKSSLCE MVSECSCFL 
      VHPNLWIRQ AVVGFITTT TKALSILDV QCKIIPNLS 
      GYMKYPLIQ IDKPELLLE SLDNPIPRN IYDSVVTYT 
      ELDVLVRML KDRKYAREA ANIGRVTTL ESWYTPSIK 
      NLFRRLEAN GLTEAMEDY FLKMEHHLK KINKHRMAT 
      KTNYGDGRI ELGSTNGQI KSHVFYLGE SQKGDFIAD 
      RRLHRTNTS GSIDTNVNS DWNYSSDLV TRHSESTNS 
      GGMSSRSTS SRPSSPPPD PHTPFISTD PSSNISLHE 
      RSYIQYRRS SCYLELNKL KIKQQEHYL EALRGKDWA 
      EQTAWLPQL PPPEWHSRG VLVAHLHEH RGTVNRLCT 
      IPDKPLFAS CSADGYVRL WDCAKMEGK NIANRSKQH 
      FKTPSGVGV TSMSVCDGG QSLGAATLD GAINILRID 
      PSGNKMNMF QTRQLNLDE EGFAVDVQC LDSGFQSVL 
      VYATLYGSI IGWDLRTPG IAWKLENSL EKGFITSFC 
      VDSHQSLLT LGTSIGHYK AWDLRFQLP INSFEQPGT 
      EKIKKVISH PTEPSWIIS SVHGNNKIL MWNLETKSL 
      EKILWGSSA PPLSQSQGP SNSVCALLA GCIDRNPFV 
      LSGGTDQRL RFWNLESPE KSHIVAPAA NDHIGSNIT 
      YESRLIDGF HVIYENPES DRGKESSEE GPRGGPEQP 
      PAGHRDCIT DITLCKASQ CFMVSSSRD GIIKVWK
    CDS sequence
    • >TC012433 RA
      atgggtaac cagctgata ggaatcgcc ccctcgcag 
      atcctcccg gtcgagcgc tacttaacg gagcacccg 
      gatttgaaa ttcgaccta ggtctggga agtacccgt 
      ttttttaaa gtagcgcga gccgaatcc caggagggc 
      ctagtagtg gtaaaaatc ttcgcaatc catgacccc 
      actctcccc ttatccacc tacaaagac caactggac 
      gaaatccgc aagcgtcta tcaagtgca gtgaactgt 
      atcccgttc cagaggatt attttaaca gaaaaggcc 
      ggtattctg gtccgagag ttcgttaag tatagtctg 
      tatgataga attagtacg cgtccgttt ttgaccaac 
      attgagaaa cgttggatc actttccaa atactgtac 
      gcactgcac cagtgccac aaagttggt gtctgtcac 
      ggtgatatt aagcttgag aatatcacg gtgacgtcg 
      tggaattgg gtgctgttg gtcgatttt gcgtcgttt 
      aagcccact tttgtcccg gaggataat ccggccgat 
      tattcgtat ttttttgac acgtcgagg cgacgtacg 
      tgctacata gccccggaa aggttcagc aagacgaca 
      aataatgaa aatagcgct ttgatcacc gagaccacg 
      tgcgaaagt ggggaattg accccagcg atggatatt 
      ttctcgaca gggtgcgca ttattggaa ttatggaac 
      gaactacac gtcccattt gaatactca caactacta 
      gcctacaga tctaacaaa tactccccc gaaaaacac 
      ttgaataat ttaaacaac ccctcactg aagtcacta 
      atttcaagc atgatcgaa atagaccca agcaagcga 
      ctgtcagcg gaagattac ctagcgaaa gagcgcgga 
      aaactattc ccggaatat ttcttcacg ttcttacag 
      agttacatg ctaattttt tccgctact ccgattcta 
      tcacccgac gaaaagatc ctaagactc aagagtgat 
      atcgccaat attttcaaa tttctagga cccatcaaa 
      cattcgcat gaagatgac agcgaaaag tatgaaaag 
      ggcgaaagt gaaggtttg gtcatcatt atttcattg 
      gtgacttca tgtatccgc ggcttgcac gactgtagc 
      tcgaaattg tgcagtctc gagatttta ttagaattg 
      tcaaatcat gcgaacagt gagactatc cttgaccgc 
      attctgcca tacattatg tatttggct cgggacaat 
      tcatcacgg gtcaaaatc gccgcaatt aacaccata 
      acaaagtgt ctcgagttg gtgcaacag ctgcccaag 
      tcggactct aatattttc cccgagtac gtgctgccc 
      gggctggcc cccttggcc ggggacccc aacacttgc 
      gtccgcgcg gcgtacgct aaaaacatc gcaacgttg 
      gctgagatc gccttacgg tacttggat cagatgcaa 
      agcgactgg tacgacaac aacatcaag caaaagtat 
      aatcacacg tttaactac gagttagaa ctgcaagcg 
      ctgcacgag atggtcgag caaaccgtt agtgtactg 
      ctgaccgat tcgcaagca ctggttaag caaacactg 
      atcgagaat gggatcacg aaattgtgt gtgtttttc 
      gggaagcaa aaggccaat gatgttctc ttgtcgcat 
      atgatcacg tttttgaat gataaggat gataagcag 
      ctgcggggg tcgtttttt gattgcatt gtgggggtg 
      gcggcgtat attgggtgg cattgctcg gggattttg 
      acgccgctt ttgttgcaa ggtttgacc gacccgtgc 
      gagttcgtc accaccaaa agtataaac gcaatgtca 
      gcattgacc gagttgggg ttgttgacg aagtcatca 
      ctgtgtgaa atggtctcg gaatgttcg tgttttttg 
      gttcacccg aatttgtgg atacgccaa gccgtggtt 
      gggtttata acaacgacg acgaaagcg ttgtcaatt 
      ttggacgtt caatgcaaa attataccg aatttgagc 
      ggctacatg aaatacccg ttgattcaa attgacaaa 
      ccagaattg ttactcgaa tctttggat aatccaatt 
      ccgaggaac atttacgac agtgtggtc acttatacg 
      gaactggat gtcttggtc cggatgctg aaagaccgg 
      aaatacgca cgagaggcg gcaaatatt ggccgtgtc 
      actactttg gagtcgtgg tacacaccg tcaattaaa 
      aaccttttt agacgtttg gaagccaac ggtttgacc 
      gaagcaatg gaagattat ttcctcaag atggaacac 
      catttgaaa aaaatcaac aaacacaga atggccaca 
      aaaacgaac tatggcgac ggtagaatt gagctcggt 
      agcaccaac ggtcaaatc aaaagtcac gtgttttat 
      ttgggggaa tcacagaag ggcgacttt atcgccgat 
      agaaggttg cacaggacg aacacatca gggtcaatt 
      gacacgaat gtgaactcc gattggaac tacagttct 
      gatctagtg acgagacat tcggaaagc actaattca 
      ggcgggatg tcgtcgagg tcgacgtct tcgagaccc 
      tcgtcgccg ccccctgac ccccacacg ccttttatc 
      agtaccgat ccttcgtct aatatcagt ctccacgaa 
      cggtcttac atacaatat cgcaggtcg agttgttat 
      ttggagctg aataaactg aaaatcaag cagcaagag 
      cactatctg gaggcttta aggggcaaa gactgggct 
      gaacaaact gcatggctg cctcagctg ccgccccct 
      gagtggcat tcacgcggc gttttggtg gcccacctc 
      cacgagcac agagggacg gtcaaccga ctatgtacc 
      atccccgat aaaccatta tttgcgagt tgttcagcc 
      gatggttac gtccgactt tgggattgt gcaaaaatg 
      gaagggaaa aatatcgca aatcgatcg aaacaacat 
      ttcaaaaca ccgagtggt gtgggggtc accagcatg 
      tcggtttgt gacgggggg cagagtctc ggagcagcc 
      actttggac ggggctatc aatatttta cggatcgat 
      ccgtccgga aataagatg aacatgttc caaacccgt 
      caactgaac ttagacgaa gaaggcttt gcagtggac 
      gtccagtgc ttggattct ggcttccag agcgtctta 
      gtttacgcc actttgtac ggctccatc atcggctgg 
      gacttgcga acgcctggc atcgcctgg aagctcgaa 
      aacagcctt gaaaaaggc ttcatcaca agtttttgc 
      gtcgactcg caccagagc ctcctcaca ctgggcacc 
      agcatcggc cactacaaa gcctgggat ctacgcttc 
      caactcccc ataaactca ttcgaacaa cccggcact 
      gagaaaatc aagaaagtg atcagccac ccaaccgag 
      ccctcatgg atcatctca agcgtccac ggcaacaac 
      aagatccta atgtggaac ttggagacc aagtctttg 
      gagaaaatc ctctggggc agctcggcc ccgcccctg 
      tcgcagtcg cagggcccc tcgaacagc gtgtgcgcc 
      ctgctggcg ggctgcatc gaccgcaac cccttcgtg 
      ctgtcgggg ggcaccgac caaagactc cgcttttgg 
      aatttggag tcgccggaa aagtcgcac attgtggcc 
      cccgctgcc aatgaccac atcgggagc aatatcacg 
      tacgagagt cggctgatt gacgggttt cacgtaatt 
      tacgagaac cccgagagc gacaggggg aaggagagc 
      agcgaggag gggccgcgg gggggcccg gagcagccc 
      ccagccggc cacagagac tgcattacc gacattacc 
      ctgtgcaag gcgtcgcaa tgtttcatg gtttcgtcc 
      tcacgggac ggaatcatc aaagtatgg aaataa
    mRNA sequence
    • >TC012433 RA
      gtatgtcaa aacaaatgc catttgcca tttcagtaa 
      atgacagaa gtgatacaa aatgattgt tggtttaac 
      aaaaacagt gataattta agtgagaaa tgggtaacc 
      agctgatag gaatcgccc cctcgcaga tcctcccgg 
      tcgagcgct acttaacgg agcacccgg atttgaaat 
      tcgacctag gtctgggaa gtacccgtt tttttaaag 
      tagcgcgag ccgaatccc aggagggcc tagtagtgg 
      taaaaatct tcgcaatcc atgacccca ctctcccct 
      tatccacct acaaagacc aactggacg aaatccgca 
      agcgtctat caagtgcag tgaactgta tcccgttcc 
      agaggatta ttttaacag aaaaggccg gtattctgg 
      tccgagagt tcgttaagt atagtctgt atgatagaa 
      ttagtacgc gtccgtttt tgaccaaca ttgagaaac 
      gttggatca ctttccaaa tactgtacg cactgcacc 
      agtgccaca aagttggtg tctgtcacg gtgatatta 
      agcttgaga atatcacgg tgacgtcgt ggaattggg 
      tgctgttgg tcgattttg cgtcgttta agcccactt 
      ttgtcccgg aggataatc cggccgatt attcgtatt 
      tttttgaca cgtcgaggc gacgtacgt gctacatag 
      ccccggaaa ggttcagca agacgacaa ataatgaaa 
      atagcgctt tgatcaccg agaccacgt gcgaaagtg 
      gggaattga ccccagcga tggatattt tctcgacag 
      ggtgcgcat tattggaat tatggaacg aactacacg 
      tcccatttg aatactcac aactactag cctacagat 
      ctaacaaat actcccccg aaaaacact tgaataatt 
      taaacaacc cctcactga agtcactaa tttcaagca 
      tgatcgaaa tagacccaa gcaagcgac tgtcagcgg 
      aagattacc tagcgaaag agcgcggaa aactattcc 
      cggaatatt tcttcacgt tcttacaga gttacatgc 
      taatttttt ccgctactc cgattctat cacccgacg 
      aaaagatcc taagactca agagtgata tcgccaata 
      ttttcaaat ttctaggac ccatcaaac attcgcatg 
      aagatgaca gcgaaaagt atgaaaagg gcgaaagtg 
      aaggtttgg tcatcatta tttcattgg tgacttcat 
      gtatccgcg gcttgcacg actgtagct cgaaattgt 
      gcagtctcg agattttat tagaattgt caaatcatg 
      cgaacagtg agactatcc ttgaccgca ttctgccat 
      acattatgt atttggctc gggacaatt catcacggg 
      tcaaaatcg ccgcaatta acaccataa caaagtgtc 
      tcgagttgg tgcaacagc tgcccaagt cggactcta 
      atattttcc ccgagtacg tgctgcccg ggctggccc 
      ccttggccg gggacccca acacttgcg tccgcgcgg 
      cgtacgcta aaaacatcg caacgttgg ctgagatcg 
      ccttacggt acttggatc agatgcaaa gcgactggt 
      acgacaaca acatcaagc aaaagtata atcacacgt 
      ttaactacg agttagaac tgcaagcgc tgcacgaga 
      tggtcgagc aaaccgtta gtgtactgc tgaccgatt 
      cgcaagcac tggttaagc aaacactga tcgagaatg 
      ggatcacga aattgtgtg tgtttttcg ggaagcaaa 
      aggccaatg atgttctct tgtcgcata tgatcacgt 
      ttttgaatg ataaggatg ataagcagc tgcgggggt 
      cgttttttg attgcattg tgggggtgg cggcgtata 
      ttgggtggc attgctcgg ggattttga cgccgcttt 
      tgttgcaag gtttgaccg acccgtgcg agttcgtca 
      ccaccaaaa gtataaacg caatgtcag cattgaccg 
      agttggggt tgttgacga agtcatcac tgtgtgaaa 
      tggtctcgg aatgttcgt gttttttgg ttcacccga 
      atttgtgga tacgccaag ccgtggttg ggtttataa 
      caacgacga cgaaagcgt tgtcaattt tggacgttc 
      aatgcaaaa ttataccga atttgagcg gctacatga 
      aatacccgt tgattcaaa ttgacaaac cagaattgt 
      tactcgaat ctttggata atccaattc cgaggaaca 
      tttacgaca gtgtggtca cttatacgg aactggatg 
      tcttggtcc ggatgctga aagaccgga aatacgcac 
      gagaggcgg caaatattg gccgtgtca ctactttgg 
      agtcgtggt acacaccgt caattaaaa accttttta 
      gacgtttgg aagccaacg gtttgaccg aagcaatgg 
      aagattatt tcctcaaga tggaacacc atttgaaaa 
      aaatcaaca aacacagaa tggccacaa aaacgaact 
      atggcgacg gtagaattg agctcggta gcaccaacg 
      gtcaaatca aaagtcacg tgttttatt tgggggaat 
      cacagaagg gcgacttta tcgccgata gaaggttgc 
      acaggacga acacatcag ggtcaattg acacgaatg 
      tgaactccg attggaact acagttctg atctagtga 
      cgagacatt cggaaagca ctaattcag gcgggatgt 
      cgtcgaggt cgacgtctt cgagaccct cgtcgccgc 
      cccctgacc cccacacgc cttttatca gtaccgatc 
      cttcgtcta atatcagtc tccacgaac ggtcttaca 
      tacaatatc gcaggtcga gttgttatt tggagctga 
      ataaactga aaatcaagc agcaagagc actatctgg 
      aggctttaa ggggcaaag actgggctg aacaaactg 
      catggctgc ctcagctgc cgccccctg agtggcatt 
      cacgcggcg ttttggtgg cccacctcc acgagcaca 
      gagggacgg tcaaccgac tatgtacca tccccgata 
      aaccattat ttgcgagtt gttcagccg atggttacg 
      tccgacttt gggattgtg caaaaatgg aagggaaaa 
      atatcgcaa atcgatcga aacaacatt tcaaaacac 
      cgagtggtg tgggggtca ccagcatgt cggtttgtg 
      acggggggc agagtctcg gagcagcca ctttggacg 
      gggctatca atattttac ggatcgatc cgtccggaa 
      ataagatga acatgttcc aaacccgtc aactgaact 
      tagacgaag aaggctttg cagtggacg tccagtgct 
      tggattctg gcttccaga gcgtcttag tttacgcca 
      ctttgtacg gctccatca tcggctggg acttgcgaa 
      cgcctggca tcgcctgga agctcgaaa acagccttg 
      aaaaaggct tcatcacaa gtttttgcg tcgactcgc 
      accagagcc tcctcacac tgggcacca gcatcggcc 
      actacaaag cctgggatc tacgcttcc aactcccca 
      taaactcat tcgaacaac ccggcactg agaaaatca 
      agaaagtga tcagccacc caaccgagc cctcatgga 
      tcatctcaa gcgtccacg gcaacaaca agatcctaa 
      tgtggaact tggagacca agtctttgg agaaaatcc 
      tctggggca gctcggccc cgcccctgt cgcagtcgc 
      agggcccct cgaacagcg tgtgcgccc tgctggcgg 
      gctgcatcg accgcaacc ccttcgtgc tgtcggggg 
      gcaccgacc aaagactcc gcttttgga atttggagt 
      cgccggaaa agtcgcaca ttgtggccc ccgctgcca 
      atgaccaca tcgggagca atatcacgt acgagagtc 
      ggctgattg acgggtttc acgtaattt acgagaacc 
      ccgagagcg acaggggga aggagagca gcgaggagg 
      ggccgcggg ggggcccgg agcagcccc cagccggcc 
      acagagact gcattaccg acattaccc tgtgcaagg 
      cgtcgcaat gtttcatgg tttcgtcct cacgggacg 
      gaatcatca aagtatgga aataagact gtttattga 
      ttttattta aatgtttac attattgtt acaataaac 
      gtgaaatgt caatagggg cacaccgga tttccgact 
      cggccgggg aaggcctgg gcgtccact gcgtggacg 
      cgacaaatg gccttatat cttcgattt ccgccctca 
      gagccgtac tggcgtccg taatttcgc ggtcggcgt 
      tgctggtag tagtcttcg aagccgaaa aggcgcaaa 
      aacggcggt gaaaacgaa tcagcggcc acaaaaacg 
      gatagaaat attaacaat gccgggaac actaacttc 
      aacttcatt ttgtcggtt ctaattgat aaaattcgt 
      tcgctgcaa ctatttata acatgggaa cgcgtcatt 
      atttatctt gatattctc agtagctca gtgttaagg 
      tcagtgaag aagaggcct cgcatttca agtaagtta 
      gtcagacta aacaaagga atgagac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY