General information | |||
TC034329
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 7086719..7095843 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC034329 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02917 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature potentially degenerated - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature not present - Penetrance (percentage of animals/eggs on slide): more than 80%
Comment: Larval musculature development is either disrupted or the animals die before the muscles are formed. |
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva cuticle thinner - Penetrance (percentage of animals/eggs on slide): less than 30%
Comment: most animals die potentially just before secreting a cuticle |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC034329 RA
MAATVKSVA SRLSILSNL KRLTPRTYF TYTNEPAQP IKGKEPKWT SLENAFEAL KSGDTVFVQ GAAATPIDL VTAMTEYGK SKKLKDVTV CHMHTEGPA AYCDPSCEG IFRSFSFFM GGNVRKAVA EGRGDCLPI FLSEIPLLF YKKIFQPEI ALIHVSPPD EHGYCSLGT SVDCVRAAM TNSKLIIAQ VNPKMPRTF GDGIIHISH IDVAVKVDT PLPQHGGRP PTEVESKIG KLIADNLVT DGATLQMGI GNIPDAVLA ALTNHKDLG IHSEMFAGG VVDLVEKGC VTNNRKSMH KGRIVGSFL IGTQKLYDF VDNNPFIEM LAIDYVNNT KIVAQQPSM TAINSCIEV DLTGQIVSD SIGTRMYSG FGGQVDFIR GAAESLDGL GKPIIALPS TTNKGESKI SPLIKQGAG VVTTRAHVH YVVTEHGIA YLFGKSLRQ RAHALINIA HPNHREALE KAAFERLKV MPSA
- >TC034329 RA
atggctgcc acagtaaaa agtgttgcg agtagactt tccatttta tcgaattta aaaaggctg acaccaaga acgtacttt acttacacc aatgaaccg gcacagcct attaagggc aaagagccc aaatggacc tccttagaa aatgcattt gaagcttta aagtcaggg gacaccgtt tttgtacaa ggggccgcc gcaaccccg atcgatcta gtaactgca atgaccgaa tatggaaaa tccaaaaaa ctgaaagac gttacggtg tgtcacatg cacactgag ggtcctgct gcttactgc gacccctcc tgtgaaggc attttcaga tcgttctcc tttttcatg ggaggaaac gtgcggaag gccgtagct gagggtaga ggtgactgt ttaccaatt ttcctctcg gaaattccg ctcctattt tacaagaag attttccaa ccggaaatc gcactaatc catgtttct ccacccgat gagcacggt tattgcagt ttgggcacc agtgttgac tgtgtcaga gcagccatg acaaactcc aagctgata atcgctcag gttaatcca aaaatgccc cgaactttc ggcgatggc ataatccac ataagtcac atcgacgtt gccgtgaag gttgacact cctttgcca caacacggg ggccgtccg ccaaccgaa gttgaatcg aaaatcggc aaattgatt gccgataat ttggtcaca gatggggcc actttacag atgggtatt ggtaacatt cccgatgct gttcttgca gccctaacg aatcacaag gatctcggt attcattcg gaaatgttt gccggtggt gtggttgat ttggtggaa aagggatgc gttacaaat aatcgaaag tcgatgcat aagggacgt atcgtcggg tcgttcttg atcggaacg cagaaattg tacgatttt gtggataat aacccgttc atcgaaatg ttggccatc gactacgtc aacaataca aagattgtt gcgcaacag cctagcatg actgcgatc aattcttgc atcgaagtg gatctaact ggccaaatc gtgtccgac tcgatcgga acccggatg tactccggc ttcggaggc caagtggac ttcatccga ggcgccgct gagagttta gacggctta gggaaacca attattgcc ttaccttcc acgacaaac aagggcgag agcaaaatt tcgcccttg attaaacaa ggtgccggt gtggtgacc accagggcc cacgtccac tacgtggtg accgagcac ggcatcgcc tacctgttc ggcaagtcc ctccggcag cgggcgcac gctctcatc aacatcgcc caccccaac caccgcgaa gccctcgaa aaagccgcc ttcgagcgc ctcaaagta atgcccagc gcttaa
- >TC034329 RA
atcgcttag acagagaca ccaatacaa attctccaa gtgcgaaaa ggagggcaa cacgtagtt gtcaaacat tggcaaaca cataaaaaa tcagtctta ttttcattc attcggttt cacacgaat ttgccttgc caagggagg tttagtttt tgagtgtct ttgagggat tttagaaga aaattgtga gtgatggct gccacagta aaaagtgtt gcgagtaga ctttccatt ttatcgaat ttaaaaagg ctgacacca agaacgtac tttacttac accaatgaa ccggcacag cctattaag ggcaaagag cccaaatgg acctcctta gaaaatgca tttgaagct ttaaagtca ggggacacc gtttttgta caaggggcc gccgcaacc ccgatcgat ctagtaact gcaatgacc gaatatgga aaatccaaa aaactgaaa gacgttacg gtgtgtcac atgcacact gagggtcct gctgcttac tgcgacccc tcctgtgaa ggcattttc agatcgttc tcctttttc atgggagga aacgtgcgg aaggccgta gctgagggt agaggtgac tgtttacca attttcctc tcggaaatt ccgctccta ttttacaag aagattttc caaccggaa atcgcacta atccatgtt tctccaccc gatgagcac ggttattgc agtttgggc accagtgtt gactgtgtc agagcagcc atgacaaac tccaagctg ataatcgct caggttaat ccaaaaatg ccccgaact ttcggcgat ggcataatc cacataagt cacatcgac gttgccgtg aaggttgac actcctttg ccacaacac gggggccgt ccgccaacc gaagttgaa tcgaaaatc ggcaaattg attgccgat aatttggtc acagatggg gccacttta cagatgggt attggtaac attcccgat gctgttctt gcagcccta acgaatcac aaggatctc ggtattcat tcggaaatg tttgccggt ggtgtggtt gatttggtg gaaaaggga tgcgttaca aataatcga aagtcgatg cataaggga cgtatcgtc gggtcgttc ttgatcgga acgcagaaa ttgtacgat tttgtggat aataacccg ttcatcgaa atgttggcc atcgactac gtcaacaat acaaagatt gttgcgcaa cagcctagc atgactgcg atcaattct tgcatcgaa gtggatcta actggccaa atcgtgtcc gactcgatc ggaacccgg atgtactcc ggcttcgga ggccaagtg gacttcatc cgaggcgcc gctgagagt ttagacggc ttagggaaa ccaattatt gccttacct tccacgaca aacaagggc gagagcaaa atttcgccc ttgattaaa caaggtgcc ggtgtggtg accaccagg gcccacgtc cactacgtg gtgaccgag cacggcatc gcctacctg ttcggcaag tccctccgg cagcgggcg cacgctctc atcaacatc gcccacccc aaccaccgc gaagccctc gaaaaagcc gccttcgag cgcctcaaa gtaatgccc agcgcttaa tttcatttc cacaattat tttttaccc gcaacacaa taagttaca aaagct
