Status
Please wait ...
Gene information
General information
TC034329
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012421
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid66: 4202548..4211672
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034329
    iBeetle screen iB_02917
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%
    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature potentially degenerated - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: Larval musculature development is either disrupted or the animals die before the muscles are formed.
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva cuticle thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: most animals die potentially just before secreting a cuticle
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034329 RA
      MAATVKSVA SRLSILSNL KRLTPRTYF TYTNEPAQP 
      IKGKEPKWT SLENAFEAL KSGDTVFVQ GAAATPIDL 
      VTAMTEYGK SKKLKDVTV CHMHTEGPA AYCDPSCEG 
      IFRSFSFFM GGNVRKAVA EGRGDCLPI FLSEIPLLF 
      YKKIFQPEI ALIHVSPPD EHGYCSLGT SVDCVRAAM 
      TNSKLIIAQ VNPKMPRTF GDGIIHISH IDVAVKVDT 
      PLPQHGGRP PTEVESKIG KLIADNLVT DGATLQMGI 
      GNIPDAVLA ALTNHKDLG IHSEMFAGG VVDLVEKGC 
      VTNNRKSMH KGRIVGSFL IGTQKLYDF VDNNPFIEM 
      LAIDYVNNT KIVAQQPSM TAINSCIEV DLTGQIVSD 
      SIGTRMYSG FGGQVDFIR GAAESLDGL GKPIIALPS 
      TTNKGESKI SPLIKQGAG VVTTRAHVH YVVTEHGIA 
      YLFGKSLRQ RAHALINIA HPNHREALE KAAFERLKV 
      MPSA
    CDS sequence
    • >TC034329 RA
      atggctgcc acagtaaaa agtgttgcg agtagactt 
      tccatttta tcgaattta aaaaggctg acaccaaga 
      acgtacttt acttacacc aatgaaccg gcacagcct 
      attaagggc aaagagccc aaatggacc tccttagaa 
      aatgcattt gaagcttta aagtcaggg gacaccgtt 
      tttgtacaa ggggccgcc gcaaccccg atcgatcta 
      gtaactgca atgaccgaa tatggaaaa tccaaaaaa 
      ctgaaagac gttacggtg tgtcacatg cacactgag 
      ggtcctgct gcttactgc gacccctcc tgtgaaggc 
      attttcaga tcgttctcc tttttcatg ggaggaaac 
      gtgcggaag gccgtagct gagggtaga ggtgactgt 
      ttaccaatt ttcctctcg gaaattccg ctcctattt 
      tacaagaag attttccaa ccggaaatc gcactaatc 
      catgtttct ccacccgat gagcacggt tattgcagt 
      ttgggcacc agtgttgac tgtgtcaga gcagccatg 
      acaaactcc aagctgata atcgctcag gttaatcca 
      aaaatgccc cgaactttc ggcgatggc ataatccac 
      ataagtcac atcgacgtt gccgtgaag gttgacact 
      cctttgcca caacacggg ggccgtccg ccaaccgaa 
      gttgaatcg aaaatcggc aaattgatt gccgataat 
      ttggtcaca gatggggcc actttacag atgggtatt 
      ggtaacatt cccgatgct gttcttgca gccctaacg 
      aatcacaag gatctcggt attcattcg gaaatgttt 
      gccggtggt gtggttgat ttggtggaa aagggatgc 
      gttacaaat aatcgaaag tcgatgcat aagggacgt 
      atcgtcggg tcgttcttg atcggaacg cagaaattg 
      tacgatttt gtggataat aacccgttc atcgaaatg 
      ttggccatc gactacgtc aacaataca aagattgtt 
      gcgcaacag cctagcatg actgcgatc aattcttgc 
      atcgaagtg gatctaact ggccaaatc gtgtccgac 
      tcgatcgga acccggatg tactccggc ttcggaggc 
      caagtggac ttcatccga ggcgccgct gagagttta 
      gacggctta gggaaacca attattgcc ttaccttcc 
      acgacaaac aagggcgag agcaaaatt tcgcccttg 
      attaaacaa ggtgccggt gtggtgacc accagggcc 
      cacgtccac tacgtggtg accgagcac ggcatcgcc 
      tacctgttc ggcaagtcc ctccggcag cgggcgcac 
      gctctcatc aacatcgcc caccccaac caccgcgaa 
      gccctcgaa aaagccgcc ttcgagcgc ctcaaagta 
      atgcccagc gcttaa
    mRNA sequence
    • >TC034329 RA
      atcgcttag acagagaca ccaatacaa attctccaa 
      gtgcgaaaa ggagggcaa cacgtagtt gtcaaacat 
      tggcaaaca cataaaaaa tcagtctta ttttcattc 
      attcggttt cacacgaat ttgccttgc caagggagg 
      tttagtttt tgagtgtct ttgagggat tttagaaga 
      aaattgtga gtgatggct gccacagta aaaagtgtt 
      gcgagtaga ctttccatt ttatcgaat ttaaaaagg 
      ctgacacca agaacgtac tttacttac accaatgaa 
      ccggcacag cctattaag ggcaaagag cccaaatgg 
      acctcctta gaaaatgca tttgaagct ttaaagtca 
      ggggacacc gtttttgta caaggggcc gccgcaacc 
      ccgatcgat ctagtaact gcaatgacc gaatatgga 
      aaatccaaa aaactgaaa gacgttacg gtgtgtcac 
      atgcacact gagggtcct gctgcttac tgcgacccc 
      tcctgtgaa ggcattttc agatcgttc tcctttttc 
      atgggagga aacgtgcgg aaggccgta gctgagggt 
      agaggtgac tgtttacca attttcctc tcggaaatt 
      ccgctccta ttttacaag aagattttc caaccggaa 
      atcgcacta atccatgtt tctccaccc gatgagcac 
      ggttattgc agtttgggc accagtgtt gactgtgtc 
      agagcagcc atgacaaac tccaagctg ataatcgct 
      caggttaat ccaaaaatg ccccgaact ttcggcgat 
      ggcataatc cacataagt cacatcgac gttgccgtg 
      aaggttgac actcctttg ccacaacac gggggccgt 
      ccgccaacc gaagttgaa tcgaaaatc ggcaaattg 
      attgccgat aatttggtc acagatggg gccacttta 
      cagatgggt attggtaac attcccgat gctgttctt 
      gcagcccta acgaatcac aaggatctc ggtattcat 
      tcggaaatg tttgccggt ggtgtggtt gatttggtg 
      gaaaaggga tgcgttaca aataatcga aagtcgatg 
      cataaggga cgtatcgtc gggtcgttc ttgatcgga 
      acgcagaaa ttgtacgat tttgtggat aataacccg 
      ttcatcgaa atgttggcc atcgactac gtcaacaat 
      acaaagatt gttgcgcaa cagcctagc atgactgcg 
      atcaattct tgcatcgaa gtggatcta actggccaa 
      atcgtgtcc gactcgatc ggaacccgg atgtactcc 
      ggcttcgga ggccaagtg gacttcatc cgaggcgcc 
      gctgagagt ttagacggc ttagggaaa ccaattatt 
      gccttacct tccacgaca aacaagggc gagagcaaa 
      atttcgccc ttgattaaa caaggtgcc ggtgtggtg 
      accaccagg gcccacgtc cactacgtg gtgaccgag 
      cacggcatc gcctacctg ttcggcaag tccctccgg 
      cagcgggcg cacgctctc atcaacatc gcccacccc 
      aaccaccgc gaagccctc gaaaaagcc gccttcgag 
      cgcctcaaa gtaatgccc agcgcttaa tttcatttc 
      cacaattat tttttaccc gcaacacaa taagttaca 
      aaagct