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Gene information
General information
TC012408 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid66: 3864564..3870408
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012408
    iBeetle screen iB_02916
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012408 RA
      MYKLIMKFK NYFTKSKCP GQTPPPPQS SSPEVPATK 
      GPSMTAPPT PNIIRKILP LAAFSVAFA IVMTVLILY 
      MDNTAMRHY QFRVNMSQD YELLSVSQD NPQLITYIR 
      EVHMQPAIE PHHKPLESQ ATGPPEDTA YVLKLLNNK 
      RDGVFVESG AYSDGKTSK TEWLEKKLG WRGLLIQSD 
      TRHYFSLRR HNRVRSQAI HACLSSMPY PKEVTFHQE 
      NEVKINSVV DDPDWFTTR IKCFPLFSL LLAMNVTTI 
      DYLSLESAG TELQVLETI PFERVKIEV IGVHLLASE 
      AERDTIKKF LAMKQYAFM QGFNHTYIF MMNRVKI
    CDS sequence
    • >TC012408 RA
      atgtacaaa cttattatg aaattcaaa aactacttc 
      accaagtcg aagtgcccg ggccagacg ccgccgccg 
      ccccagagc agctcaccc gaggtccct gcgacaaag 
      ggccccagc atgacagcg ccacccact cccaacatt 
      atcaggaaa atcttaccc ctggccgcg ttttcggtg 
      gcttttgcc atcgtcatg acagtcctt atcctctat 
      atggataac acagccatg cgacactac cagtttcgg 
      gtcaacatg agccaggat tacgaactt ttgagcgtc 
      tcacaagac aatccacaa cttatcacc tacatcagg 
      gaggtgcac atgcagccc gccattgag ccccaccac 
      aagccgctc gagtcgcag gccacgggg cccccggaa 
      gacaccgcc tacgtgctc aaactactc aacaataag 
      cgggacggc gtgttcgtg gagtcgggg gcgtacagc 
      gacggcaag acgtcgaaa accgagtgg ctggagaag 
      aagctgggg tggcggggg ctcctgatc cagtccgac 
      acccggcac tacttcagc ctgcgacgg cacaaccgg 
      gtgcggtcc caggcgatc cacgcctgc ctcagctcc 
      atgccgtac cccaaagaa gtgacattc caccaagag 
      aacgaggtc aaaatcaac agcgtggtg gacgacccc 
      gactggttc accacgcgg atcaagtgt ttcccgctg 
      ttctcgctg ctgctcgcg atgaacgtc acgacgatc 
      gactacctg agcctggag tcggccggc accgagctg 
      caggtgctg gagacgatt ccgttcgaa agggtgaag 
      atcgaagtg attggggtg catttgctg gccagtgag 
      gcggagaga gacacgatc aagaagttt ctggcgatg 
      aagcagtac gccttcatg cagggcttc aaccacact 
      tatattttt atgatgaat agggtgaaa atttga
    mRNA sequence
    • >TC012408 RA
      ccattgttc taattttag cacaaatgg ttaatttaa 
      ccctgcaac agcttgcag acccgtcta cctgctgtt 
      attacaggt ttcagcaaa caatagcta aggaaatat 
      cgacccgaa gacaccgaa aaatttcgg ctgttcacc 
      ccctcacaa atcggaaat cgccaaggc cggaacacc 
      atatttatg gtaatttgt tcctgaaag tcacgttct 
      gatttgtat cgtaatggg gtggaaatt taaaccgat 
      tcggacctg tcacagctc aaattagtt tccaagttt 
      tggccaaat cgaacgaaa ttctgatta acccccaga 
      gacgcattc ttttttgta gacaaatta aaattattg 
      agtatcttg ttaccgaaa agataatta aaagtttga 
      aagcatttt tgcgcaatt tatgggttg tgtgcaaaa 
      agcccaaac cgcgaaatt aggccaaag tattggttt 
      acaaatggg caatttttg tgaatttct ctgggaata 
      aattacggc gagcgcacg aacgagtag aaagagaaa 
      cattgttat tcttgatgt ctctccaac aaggaacaa 
      ggtgggagt gcaaatcaa taaagtcga aaatcgtgc 
      atttcgctt tgtatttcg agccgaacg cgaccgtag 
      cgcacttca agcacctgg aaagacagg atgtgccat 
      cgggtcatt tcattgtat tgctcatta tttcacctt 
      taaaccgaa aattatgta cgaattaaa aaaccgtcc 
      caattaacc cgaccgtct aattgtgac gatgtacaa 
      acttattat gaaattcaa aaactactt caccaagtc 
      gaagtgccc gggccagac gccgccgcc gccccagag 
      cagctcacc cgaggtccc tgcgacaaa gggccccag 
      catgacagc gccacccac tcccaacat tatcaggaa 
      aatcttacc cctggccgc gttttcggt ggcttttgc 
      catcgtcat gacagtcct tatcctcta tatggataa 
      cacagccat gcgacacta ccagtttcg ggtcaacat 
      gagccagga ttacgaact tttgagcgt ctcacaaga 
      caatccaca acttatcac ctacatcag ggaggtgca 
      catgcagcc cgccattga gccccacca caagccgct 
      cgagtcgca ggccacggg gcccccgga agacaccgc 
      ctacgtgct caaactact caacaataa gcgggacgg 
      cgtgttcgt ggagtcggg ggcgtacag cgacggcaa 
      gacgtcgaa aaccgagtg gctggagaa gaagctggg 
      gtggcgggg gctcctgat ccagtccga cacccggca 
      ctacttcag cctgcgacg gcacaaccg ggtgcggtc 
      ccaggcgat ccacgcctg cctcagctc catgccgta 
      ccccaaaga agtgacatt ccaccaaga gaacgaggt 
      caaaatcaa cagcgtggt ggacgaccc cgactggtt 
      caccacgcg gatcaagtg tttcccgct gttctcgct 
      gctgctcgc gatgaacgt cacgacgat cgactacct 
      gagcctgga gtcggccgg caccgagct gcaggtgct 
      ggagacgat tccgttcga aagggtgaa gatcgaagt 
      gattggggt gcatttgct ggccagtga ggcggagag 
      agacacgat caagaagtt tctggcgat gaagcagta 
      cgccttcat gcagggctt caaccacac ttatatttt 
      tatgatgaa tagggtgaa aatttgagg ggtaattgt 
      cacgtagaa gactgaagc gcgcaaatg aatgctgat 
      caccgtatt tttatacct atgtgatag gacttggtt 
      tttatttcg ctgtcacta gtgcctttg tagcgttcc 
      aattgttga gatgcattc ccgttatac aactgattt 
      ttatgcatt tattttagg attaatgat tagattagg 
      cgacaatta agtacttat gcttgattt catttgtac 
      aattctcca tgacatttt tgtacttat tctttatta 
      aataaaggt cacgatatg agttgcttt ttgcatgca 
      ccaggagta ttaaggtag aaatttatt tttataaat 
      atattttct tgtacacta atacaattt ttatgtaac 
      aactatatc aataaaaat tggaattta tacaattta 
      tcaaatata atttctagt gattatttg gatagatca 
      caattatta tttttgttc agtaaaaat tatatcgtg 
      ctactctat tctccttat aatagctga aaaaacaaa 
      acttggtta aatatgtgc accacggag gactaaaac 
      aggaggcga aacgagaaa atttgccgc aaatctcaa 
      tttctccga gaaaaacgc gttttgagt tttgaattc 
      gcacttctt acggcgaaa caaccttca aacgaacaa 
      aaaaaatat atttttttt taatttaat taagttaaa 
      tttttacga aaaataata aacaaaatc cttttttac 
      aattagatg ttcttaaaa aaaacaatt attttccag 
      gtcagtttt atctcattt taaatattc gtggccgtt 
      gtaacaaat ctgttcata aaaaaataa aacaaattg 
      tgtgcagtt tttatatta aaaataaaa taacaaaaa 
      aattttttt tgttttatt atgacaaaa taacgtcca 
      caatcgcat gaaataaat acatgaata acataaata 
      aataacaaa atgtgtgca gtctttata ttaaaaata 
      aaatagcaa tttttttta ttatgacaa tgtttactt 
      cacattata ccatataca aggtgattc tcttagggc 
      ctaagttag caaatat