Status
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Gene information
General information
TC012368 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5783937..5792819
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012368
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02914

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva segment random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012368 RA
      MGAKVCKPC KKRKANTFQ GGSILDRVI SQASNQDQC 
      LLYKLANYK KGGELIDAY NQGGQAEVE KLIREQFGQ 
      LMYQEGKGQ IINRSEYLR WKFRDHEQV ILPIEASLS 
      RYDPLAKWN DHEACWQMQ FRGSLGESL LHVLIICDT 
      KIHTRLART LIKCFPKLA LDVVEGEEY LGASALHLA 
      IAYNNNELV QDLVEAGAN VNQRAIGSF FLPRDQQRQ 
      KPAKHTDYE GLAYLGEYP LAWAACCAN ESVYNLLLD 
      SGAHPDYQD NFGNMILHM VVVCDKLDM FGYALRHPK 
      LPASNGIVN KAGLTPLTL ACKLGRAEV FREMLELSA 
      KEFWRYSNI TCSAYFLNA LDTLLPDGR TNWNSALFI 
      ILNGTKEEH LAMLDGGII QRLLEEKWK TFARNQFLK 
      RLLILVVHL LFLSLAVYL RPDDPDESL LTWSDDVTL 
      IARYVCEVG TILGVLSYL VLQQGDEIR NQGLTAFLK 
      QQLNSPPKL IFLISNFLI LACIPCRLY GDKETEEAI 
      LCFAVPGSW FLLMFFAGA VRLTGPFVT MIYSMITGD 
      MLTFGIIYT VFLFGFSQS FYFLYKGFP GVKTSLYNT 
      YMSTWMALF QITLGNYEY SELSATTYP AVSKTVFAI 
      FMVFVPILL LNMLIAMMG NTYAHVIEQ SEKEWVKQW 
      AKIVIALER AIPQSDAQH YLQEYSISL GPSEQDPST 
      EKRGVLVIK SKSKTRAKQ RKGAVANWK RVGKVTINA 
      LKKRGLTGE EMRCLMWGR ESINTPVKT KKPVKDPLL 
      DPQGPNLTG GFGDALTTA LDVMTFTHD LDIVGASQG 
      LNLATNPKP VPPTTTASA VTVNNQINS ALNAQQKAV 
      AGAGVGALA MIGTVTQLT DSQGYIMQN SVKKEEKIV 
      ATLEDPFRE LVINANDSN CDPEKLKML ALSAANLKD 
      VEELSVAKP QTKSVKSLA GIFAGTETF VRKVEETIK 
      KKYAALDPS DSEGFGEPP LLGKISRTR RAKSANLRN 
      SSARSKASD KKKLVAGSQ SSSTDTVNN EINEKNIEN 
      SDLDYAEER IKLVKESLK QVVDVAQIR PINIDVALE 
      EQVSVTISE TMRVQGSGD GAEVQSSNV KPKRKKRSK 
      TAKNNKVAP AVEPRPCRS ANDHFDSDD SESPDPLEP 
      WSTKKIANM NKILAWEND QDSM
    CDS sequence
    • >TC012368 RA
      atgggtgca aaagtgtgc aaaccgtgt aaaaagcgc 
      aaagccaac accttccaa gggggctcc atcctcgac 
      cgggtcatc agccaagcc tcaaaccaa gaccagtgc 
      ctcctttac aaattagca aattacaag aaaggcggc 
      gaactgatc gacgcgtac aaccaaggg ggccaagcc 
      gaggtggaa aaattaata cgcgagcaa ttcggccag 
      ttgatgtac caggaaggc aaaggccag ataatcaac 
      cgctcggag tatctcagg tggaaattt cgcgaccac 
      gagcaggtc attctccca atcgaggcc tccttgagc 
      cgatacgac cccctggcc aagtggaac gaccacgag 
      gcgtgctgg cagatgcag ttcaggggg tctttaggc 
      gagtctttg ctgcacgtc ctcataatt tgcgacacg 
      aaaattcac acaagactg gcgcggact ctgatcaag 
      tgtttcccg aagctggcc ctcgacgtt gtcgagggc 
      gaggagtat ctaggggcg agtgctttg cacctagcg 
      atcgcttac aacaacaat gagctagtc caggacttg 
      gtcgaagct ggtgctaac gtgaaccag cgcgccatt 
      gggagcttt ttcctcccg agggaccaa caacgccaa 
      aaacccgca aaacacacc gactacgag gggctcgcc 
      tacttgggg gaataccct ctggcctgg gccgcgtgt 
      tgcgccaac gaaagcgtc tacaatctg ttgctggac 
      agtggggcg caccccgac tatcaggac aattttgga 
      aacatgatc ttgcacatg gtggtggtt tgcgacaag 
      ttggacatg ttcggctac gccttgaga cacccgaaa 
      ctaccggca agcaacgga attgtgaat aaagcaggt 
      ctaactccc ctaacgctg gcttgtaag ctgggtcgg 
      gctgaggtt ttccgcgaa atgttggag ctttcagcc 
      aaggagttt tggaggtat agtaacatc acctgctcg 
      gcgtatttt ttgaacgct ttggatacg ctgctaccg 
      gacgggcgc acaaactgg aattcggcc cttttcatt 
      attttaaac ggaaccaag gaagagcat ttagccatg 
      cttgacggt gggattatc caacgactg ttggaggaa 
      aaatggaaa acattcgca cggaaccaa tttttgaaa 
      cggcttctt atcctcgtt gtgcatttg ttgtttctg 
      tctttggcg gtttacttg cgaccagac gacccagac 
      gagtctctt ctcacctgg tctgatgat gttacactg 
      atagctaga tatgtgtgt gaagtgggc acgattttg 
      ggcgtcctc agttatctg gttttgcaa caaggggac 
      gaaatccgt aatcaggga cttactgcg ttcttgaaa 
      caacaactg aattcaccc ccgaaatta attttcctc 
      atttcgaat ttcctaatt ttggcgtgc attccatgc 
      aggttgtat ggcgataag gaaaccgag gaggctatt 
      ctgtgtttt gcggtacct gggtcttgg tttcttctc 
      atgtttttt gcaggggcc gttcgtttg actggtcct 
      tttgtcaca atgatttac agcatgatt accggcgat 
      atgttgact tttggcatc atttacacc gttttctta 
      tttggtttt tcgcagtcg ttctatttc ctgtataag 
      ggattcccc ggggtcaaa acttcctta tacaacacg 
      tacatgtcg acttggatg gccttattc caaattact 
      ttgggcaat tatgaatat tctgagttg agtgccacc 
      acataccca gcggtcagc aaaacagtc tttgccatc 
      ttcatggtg ttcgtccca attttgctg ctcaacatg 
      ttaatcgcc atgatgggc aacacttac gcccatgta 
      atcgagcaa agcgagaaa gaatgggtc aaacaatgg 
      gcaaaaatc gtcatcgcc ctggaacgg gcaatccca 
      caatccgac gcccaacat tacttacaa gagtacagt 
      atttcgctg gggcccagt gaacaggac cccagtacc 
      gaaaaacgc ggagttttg gtcataaaa tcaaagagt 
      aaaacgcgg gcaaaacaa cgaaaaggg gccgtggcc 
      aattggaag cgggtgggc aaagtaacg ataaacgcg 
      ctcaaaaaa cgcggctta accggtgaa gaaatgcgc 
      tgcttgatg tggggccgc gagtctatt aatacaccg 
      gtcaaaaca aagaagcct gtgaaggac ccactgctg 
      gacccgcaa gggccgaat ttaacgggt ggcttcggc 
      gatgcttta accactgct ttggatgtg atgacgttc 
      acgcacgat ttggatatt gtgggggct tcccagggc 
      ctgaatttg gccacgaac cctaaaccc gtccctccc 
      acaacaaca gcgagtgcc gtgactgtg aataaccag 
      ataaattcg gctctgaac gcgcagcaa aaggccgta 
      gctggggct ggagttggt gctttggct atgataggc 
      acagtgacc caactcacc gactctcaa ggctacatt 
      atgcaaaac agtgtgaaa aaagaagaa aaaattgtc 
      gcaactttg gaggaccct ttcagggaa cttgtcata 
      aacgctaat gacagtaac tgcgacccg gaaaaatta 
      aaaatgctt gcgctatcg gccgccaat ttgaaagac 
      gttgaagag ttatcggtg gccaagcca caaacgaaa 
      agtgttaag agtctagcg gggattttc gccgggact 
      gagactttt gtgaggaaa gttgaagaa acaataaag 
      aagaaatat gcggctttg gatccaagt gatagtgaa 
      ggttttggc gaacctcca ctattaggg aaaatatcc 
      aggactaga agggccaaa tcggcaaac ttgcgcaac 
      tcttcagcg cgatctaaa gcttcagat aagaaaaaa 
      ctggtcgcg gggtcacag tcaagctcc actgatacg 
      gtcaataac gaaattaat gaaaagaat atcgaaaat 
      tctgactta gactatgcc gaagagagg atcaagctg 
      gtgaaagag tcgttaaag caggtggtt gacgttgcg 
      caaattcgg ccgattaac atcgatgtg gccctcgag 
      gagcaagtg tcggtcaca atttcggaa acgatgcga 
      gtgcagggc tccggggac ggggccgaa gtccagtca 
      tccaacgtc aaaccaaag cgcaaaaaa cggagcaaa 
      acagccaaa aacaacaag gttgcgccc gcggttgaa 
      ccgagacct tgtcggtca gctaacgac cacttcgat 
      tccgacgat tctgaatca cctgaccct ttagaacct 
      tggagtaca aaaaaaatc gcaaatatg aacaaaatt 
      ctagcttgg gagaacgac caagacagc atgtaa
    mRNA sequence
    • >TC012368 RA
      gatcacaac tgttttgaa atgggtgca aaagtgtgc 
      aaaccgtgt aaaaagcgc aaagccaac accttccaa 
      gggggctcc atcctcgac cgggtcatc agccaagcc 
      tcaaaccaa gaccagtgc ctcctttac aaattagca 
      aattacaag aaaggcggc gaactgatc gacgcgtac 
      aaccaaggg ggccaagcc gaggtggaa aaattaata 
      cgcgagcaa ttcggccag ttgatgtac caggaaggc 
      aaaggccag ataatcaac cgctcggag tatctcagg 
      tggaaattt cgcgaccac gagcaggtc attctccca 
      atcgaggcc tccttgagc cgatacgac cccctggcc 
      aagtggaac gaccacgag gcgtgctgg cagatgcag 
      ttcaggggg tctttaggc gagtctttg ctgcacgtc 
      ctcataatt tgcgacacg aaaattcac acaagactg 
      gcgcggact ctgatcaag tgtttcccg aagctggcc 
      ctcgacgtt gtcgagggc gaggagtat ctaggggcg 
      agtgctttg cacctagcg atcgcttac aacaacaat 
      gagctagtc caggacttg gtcgaagct ggtgctaac 
      gtgaaccag cgcgccatt gggagcttt ttcctcccg 
      agggaccaa caacgccaa aaacccgca aaacacacc 
      gactacgag gggctcgcc tacttgggg gaataccct 
      ctggcctgg gccgcgtgt tgcgccaac gaaagcgtc 
      tacaatctg ttgctggac agtggggcg caccccgac 
      tatcaggac aattttgga aacatgatc ttgcacatg 
      gtggtggtt tgcgacaag ttggacatg ttcggctac 
      gccttgaga cacccgaaa ctaccggca agcaacgga 
      attgtgaat aaagcaggt ctaactccc ctaacgctg 
      gcttgtaag ctgggtcgg gctgaggtt ttccgcgaa 
      atgttggag ctttcagcc aaggagttt tggaggtat 
      agtaacatc acctgctcg gcgtatttt ttgaacgct 
      ttggatacg ctgctaccg gacgggcgc acaaactgg 
      aattcggcc cttttcatt attttaaac ggaaccaag 
      gaagagcat ttagccatg cttgacggt gggattatc 
      caacgactg ttggaggaa aaatggaaa acattcgca 
      cggaaccaa tttttgaaa cggcttctt atcctcgtt 
      gtgcatttg ttgtttctg tctttggcg gtttacttg 
      cgaccagac gacccagac gagtctctt ctcacctgg 
      tctgatgat gttacactg atagctaga tatgtgtgt 
      gaagtgggc acgattttg ggcgtcctc agttatctg 
      gttttgcaa caaggggac gaaatccgt aatcaggga 
      cttactgcg ttcttgaaa caacaactg aattcaccc 
      ccgaaatta attttcctc atttcgaat ttcctaatt 
      ttggcgtgc attccatgc aggttgtat ggcgataag 
      gaaaccgag gaggctatt ctgtgtttt gcggtacct 
      gggtcttgg tttcttctc atgtttttt gcaggggcc 
      gttcgtttg actggtcct tttgtcaca atgatttac 
      agcatgatt accggcgat atgttgact tttggcatc 
      atttacacc gttttctta tttggtttt tcgcagtcg 
      ttctatttc ctgtataag ggattcccc ggggtcaaa 
      acttcctta tacaacacg tacatgtcg acttggatg 
      gccttattc caaattact ttgggcaat tatgaatat 
      tctgagttg agtgccacc acataccca gcggtcagc 
      aaaacagtc tttgccatc ttcatggtg ttcgtccca 
      attttgctg ctcaacatg ttaatcgcc atgatgggc 
      aacacttac gcccatgta atcgagcaa agcgagaaa 
      gaatgggtc aaacaatgg gcaaaaatc gtcatcgcc 
      ctggaacgg gcaatccca caatccgac gcccaacat 
      tacttacaa gagtacagt atttcgctg gggcccagt 
      gaacaggac cccagtacc gaaaaacgc ggagttttg 
      gtcataaaa tcaaagagt aaaacgcgg gcaaaacaa 
      cgaaaaggg gccgtggcc aattggaag cgggtgggc 
      aaagtaacg ataaacgcg ctcaaaaaa cgcggctta 
      accggtgaa gaaatgcgc tgcttgatg tggggccgc 
      gagtctatt aatacaccg gtcaaaaca aagaagcct 
      gtgaaggac ccactgctg gacccgcaa gggccgaat 
      ttaacgggt ggcttcggc gatgcttta accactgct 
      ttggatgtg atgacgttc acgcacgat ttggatatt 
      gtgggggct tcccagggc ctgaatttg gccacgaac 
      cctaaaccc gtccctccc acaacaaca gcgagtgcc 
      gtgactgtg aataaccag ataaattcg gctctgaac 
      gcgcagcaa aaggccgta gctggggct ggagttggt 
      gctttggct atgataggc acagtgacc caactcacc 
      gactctcaa ggctacatt atgcaaaac agtgtgaaa 
      aaagaagaa aaaattgtc gcaactttg gaggaccct 
      ttcagggaa cttgtcata aacgctaat gacagtaac 
      tgcgacccg gaaaaatta aaaatgctt gcgctatcg 
      gccgccaat ttgaaagac gttgaagag ttatcggtg 
      gccaagcca caaacgaaa agtgttaag agtctagcg 
      gggattttc gccgggact gagactttt gtgaggaaa 
      gttgaagaa acaataaag aagaaatat gcggctttg 
      gatccaagt gatagtgaa ggttttggc gaacctcca 
      ctattaggg aaaatatcc aggactaga agggccaaa 
      tcggcaaac ttgcgcaac tcttcagcg cgatctaaa 
      gcttcagat aagaaaaaa ctggtcgcg gggtcacag 
      tcaagctcc actgatacg gtcaataac gaaattaat 
      gaaaagaat atcgaaaat tctgactta gactatgcc 
      gaagagagg atcaagctg gtgaaagag tcgttaaag 
      caggtggtt gacgttgcg caaattcgg ccgattaac 
      atcgatgtg gccctcgag gagcaagtg tcggtcaca 
      atttcggaa acgatgcga gtgcagggc tccggggac 
      ggggccgaa gtccagtca tccaacgtc aaaccaaag 
      cgcaaaaaa cggagcaaa acagccaaa aacaacaag 
      gttgcgccc gcggttgaa ccgagacct tgtcggtca 
      gctaacgac cacttcgat tccgacgat tctgaatca 
      cctgaccct ttagaacct tggagtaca aaaaaaatc 
      gcaaatatg aacaaaatt ctagcttgg gagaacgac 
      caagacagc atgtaatct ttcaaacct gtcaaataa 
      attctattt ttttgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY