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Gene information
General information
TC012368 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid66: 2899766..2908648
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012368
    iBeetle screen iB_02914
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva segment random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012368 RA
      MGAKVCKPC KKRKANTFQ GGSILDRVI SQASNQDQC 
LLYKLANYK KGGELIDAY NQGGQAEVE KLIREQFGQ 
LMYQEGKGQ IINRSEYLR WKFRDHEQV ILPIEASLS 
RYDPLAKWN DHEACWQMQ FRGSLGESL LHVLIICDT 
KIHTRLART LIKCFPKLA LDVVEGEEY LGASALHLA 
IAYNNNELV QDLVEAGAN VNQRAIGSF FLPRDQQRQ 
KPAKHTDYE GLAYLGEYP LAWAACCAN ESVYNLLLD 
SGAHPDYQD NFGNMILHM VVVCDKLDM FGYALRHPK 
LPASNGIVN KAGLTPLTL ACKLGRAEV FREMLELSA 
KEFWRYSNI TCSAYFLNA LDTLLPDGR TNWNSALFI 
ILNGTKEEH LAMLDGGII QRLLEEKWK TFARNQFLK 
RLLILVVHL LFLSLAVYL RPDDPDESL LTWSDDVTL 
IARYVCEVG TILGVLSYL VLQQGDEIR NQGLTAFLK 
QQLNSPPKL IFLISNFLI LACIPCRLY GDKETEEAI 
LCFAVPGSW FLLMFFAGA VRLTGPFVT MIYSMITGD 
MLTFGIIYT VFLFGFSQS FYFLYKGFP GVKTSLYNT 
YMSTWMALF QITLGNYEY SELSATTYP AVSKTVFAI 
FMVFVPILL LNMLIAMMG NTYAHVIEQ SEKEWVKQW 
AKIVIALER AIPQSDAQH YLQEYSISL GPSEQDPST 
EKRGVLVIK SKSKTRAKQ RKGAVANWK RVGKVTINA 
LKKRGLTGE EMRCLMWGR ESINTPVKT KKPVKDPLL 
DPQGPNLTG GFGDALTTA LDVMTFTHD LDIVGASQG 
LNLATNPKP VPPTTTASA VTVNNQINS ALNAQQKAV 
AGAGVGALA MIGTVTQLT DSQGYIMQN SVKKEEKIV 
ATLEDPFRE LVINANDSN CDPEKLKML ALSAANLKD 
VEELSVAKP QTKSVKSLA GIFAGTETF VRKVEETIK 
KKYAALDPS DSEGFGEPP LLGKISRTR RAKSANLRN 
SSARSKASD KKKLVAGSQ SSSTDTVNN EINEKNIEN 
SDLDYAEER IKLVKESLK QVVDVAQIR PINIDVALE 
EQVSVTISE TMRVQGSGD GAEVQSSNV KPKRKKRSK 
TAKNNKVAP AVEPRPCRS ANDHFDSDD SESPDPLEP 
WSTKKIANM NKILAWEND QDSM
    CDS sequence
    • >TC012368 RA
      atgggtgca aaagtgtgc aaaccgtgt aaaaagcgc 
aaagccaac accttccaa gggggctcc atcctcgac 
cgggtcatc agccaagcc tcaaaccaa gaccagtgc 
ctcctttac aaattagca aattacaag aaaggcggc 
gaactgatc gacgcgtac aaccaaggg ggccaagcc 
gaggtggaa aaattaata cgcgagcaa ttcggccag 
ttgatgtac caggaaggc aaaggccag ataatcaac 
cgctcggag tatctcagg tggaaattt cgcgaccac 
gagcaggtc attctccca atcgaggcc tccttgagc 
cgatacgac cccctggcc aagtggaac gaccacgag 
gcgtgctgg cagatgcag ttcaggggg tctttaggc 
gagtctttg ctgcacgtc ctcataatt tgcgacacg 
aaaattcac acaagactg gcgcggact ctgatcaag 
tgtttcccg aagctggcc ctcgacgtt gtcgagggc 
gaggagtat ctaggggcg agtgctttg cacctagcg 
atcgcttac aacaacaat gagctagtc caggacttg 
gtcgaagct ggtgctaac gtgaaccag cgcgccatt 
gggagcttt ttcctcccg agggaccaa caacgccaa 
aaacccgca aaacacacc gactacgag gggctcgcc 
tacttgggg gaataccct ctggcctgg gccgcgtgt 
tgcgccaac gaaagcgtc tacaatctg ttgctggac 
agtggggcg caccccgac tatcaggac aattttgga 
aacatgatc ttgcacatg gtggtggtt tgcgacaag 
ttggacatg ttcggctac gccttgaga cacccgaaa 
ctaccggca agcaacgga attgtgaat aaagcaggt 
ctaactccc ctaacgctg gcttgtaag ctgggtcgg 
gctgaggtt ttccgcgaa atgttggag ctttcagcc 
aaggagttt tggaggtat agtaacatc acctgctcg 
gcgtatttt ttgaacgct ttggatacg ctgctaccg 
gacgggcgc acaaactgg aattcggcc cttttcatt 
attttaaac ggaaccaag gaagagcat ttagccatg 
cttgacggt gggattatc caacgactg ttggaggaa 
aaatggaaa acattcgca cggaaccaa tttttgaaa 
cggcttctt atcctcgtt gtgcatttg ttgtttctg 
tctttggcg gtttacttg cgaccagac gacccagac 
gagtctctt ctcacctgg tctgatgat gttacactg 
atagctaga tatgtgtgt gaagtgggc acgattttg 
ggcgtcctc agttatctg gttttgcaa caaggggac 
gaaatccgt aatcaggga cttactgcg ttcttgaaa 
caacaactg aattcaccc ccgaaatta attttcctc 
atttcgaat ttcctaatt ttggcgtgc attccatgc 
aggttgtat ggcgataag gaaaccgag gaggctatt 
ctgtgtttt gcggtacct gggtcttgg tttcttctc 
atgtttttt gcaggggcc gttcgtttg actggtcct 
tttgtcaca atgatttac agcatgatt accggcgat 
atgttgact tttggcatc atttacacc gttttctta 
tttggtttt tcgcagtcg ttctatttc ctgtataag 
ggattcccc ggggtcaaa acttcctta tacaacacg 
tacatgtcg acttggatg gccttattc caaattact 
ttgggcaat tatgaatat tctgagttg agtgccacc 
acataccca gcggtcagc aaaacagtc tttgccatc 
ttcatggtg ttcgtccca attttgctg ctcaacatg 
ttaatcgcc atgatgggc aacacttac gcccatgta 
atcgagcaa agcgagaaa gaatgggtc aaacaatgg 
gcaaaaatc gtcatcgcc ctggaacgg gcaatccca 
caatccgac gcccaacat tacttacaa gagtacagt 
atttcgctg gggcccagt gaacaggac cccagtacc 
gaaaaacgc ggagttttg gtcataaaa tcaaagagt 
aaaacgcgg gcaaaacaa cgaaaaggg gccgtggcc 
aattggaag cgggtgggc aaagtaacg ataaacgcg 
ctcaaaaaa cgcggctta accggtgaa gaaatgcgc 
tgcttgatg tggggccgc gagtctatt aatacaccg 
gtcaaaaca aagaagcct gtgaaggac ccactgctg 
gacccgcaa gggccgaat ttaacgggt ggcttcggc 
gatgcttta accactgct ttggatgtg atgacgttc 
acgcacgat ttggatatt gtgggggct tcccagggc 
ctgaatttg gccacgaac cctaaaccc gtccctccc 
acaacaaca gcgagtgcc gtgactgtg aataaccag 
ataaattcg gctctgaac gcgcagcaa aaggccgta 
gctggggct ggagttggt gctttggct atgataggc 
acagtgacc caactcacc gactctcaa ggctacatt 
atgcaaaac agtgtgaaa aaagaagaa aaaattgtc 
gcaactttg gaggaccct ttcagggaa cttgtcata 
aacgctaat gacagtaac tgcgacccg gaaaaatta 
aaaatgctt gcgctatcg gccgccaat ttgaaagac 
gttgaagag ttatcggtg gccaagcca caaacgaaa 
agtgttaag agtctagcg gggattttc gccgggact 
gagactttt gtgaggaaa gttgaagaa acaataaag 
aagaaatat gcggctttg gatccaagt gatagtgaa 
ggttttggc gaacctcca ctattaggg aaaatatcc 
aggactaga agggccaaa tcggcaaac ttgcgcaac 
tcttcagcg cgatctaaa gcttcagat aagaaaaaa 
ctggtcgcg gggtcacag tcaagctcc actgatacg 
gtcaataac gaaattaat gaaaagaat atcgaaaat 
tctgactta gactatgcc gaagagagg atcaagctg 
gtgaaagag tcgttaaag caggtggtt gacgttgcg 
caaattcgg ccgattaac atcgatgtg gccctcgag 
gagcaagtg tcggtcaca atttcggaa acgatgcga 
gtgcagggc tccggggac ggggccgaa gtccagtca 
tccaacgtc aaaccaaag cgcaaaaaa cggagcaaa 
acagccaaa aacaacaag gttgcgccc gcggttgaa 
ccgagacct tgtcggtca gctaacgac cacttcgat 
tccgacgat tctgaatca cctgaccct ttagaacct 
tggagtaca aaaaaaatc gcaaatatg aacaaaatt 
ctagcttgg gagaacgac caagacagc atgtaa
    mRNA sequence
    • >TC012368 RA
      gatcacaac tgttttgaa atgggtgca aaagtgtgc 
aaaccgtgt aaaaagcgc aaagccaac accttccaa 
gggggctcc atcctcgac cgggtcatc agccaagcc 
tcaaaccaa gaccagtgc ctcctttac aaattagca 
aattacaag aaaggcggc gaactgatc gacgcgtac 
aaccaaggg ggccaagcc gaggtggaa aaattaata 
cgcgagcaa ttcggccag ttgatgtac caggaaggc 
aaaggccag ataatcaac cgctcggag tatctcagg 
tggaaattt cgcgaccac gagcaggtc attctccca 
atcgaggcc tccttgagc cgatacgac cccctggcc 
aagtggaac gaccacgag gcgtgctgg cagatgcag 
ttcaggggg tctttaggc gagtctttg ctgcacgtc 
ctcataatt tgcgacacg aaaattcac acaagactg 
gcgcggact ctgatcaag tgtttcccg aagctggcc 
ctcgacgtt gtcgagggc gaggagtat ctaggggcg 
agtgctttg cacctagcg atcgcttac aacaacaat 
gagctagtc caggacttg gtcgaagct ggtgctaac 
gtgaaccag cgcgccatt gggagcttt ttcctcccg 
agggaccaa caacgccaa aaacccgca aaacacacc 
gactacgag gggctcgcc tacttgggg gaataccct 
ctggcctgg gccgcgtgt tgcgccaac gaaagcgtc 
tacaatctg ttgctggac agtggggcg caccccgac 
tatcaggac aattttgga aacatgatc ttgcacatg 
gtggtggtt tgcgacaag ttggacatg ttcggctac 
gccttgaga cacccgaaa ctaccggca agcaacgga 
attgtgaat aaagcaggt ctaactccc ctaacgctg 
gcttgtaag ctgggtcgg gctgaggtt ttccgcgaa 
atgttggag ctttcagcc aaggagttt tggaggtat 
agtaacatc acctgctcg gcgtatttt ttgaacgct 
ttggatacg ctgctaccg gacgggcgc acaaactgg 
aattcggcc cttttcatt attttaaac ggaaccaag 
gaagagcat ttagccatg cttgacggt gggattatc 
caacgactg ttggaggaa aaatggaaa acattcgca 
cggaaccaa tttttgaaa cggcttctt atcctcgtt 
gtgcatttg ttgtttctg tctttggcg gtttacttg 
cgaccagac gacccagac gagtctctt ctcacctgg 
tctgatgat gttacactg atagctaga tatgtgtgt 
gaagtgggc acgattttg ggcgtcctc agttatctg 
gttttgcaa caaggggac gaaatccgt aatcaggga 
cttactgcg ttcttgaaa caacaactg aattcaccc 
ccgaaatta attttcctc atttcgaat ttcctaatt 
ttggcgtgc attccatgc aggttgtat ggcgataag 
gaaaccgag gaggctatt ctgtgtttt gcggtacct 
gggtcttgg tttcttctc atgtttttt gcaggggcc 
gttcgtttg actggtcct tttgtcaca atgatttac 
agcatgatt accggcgat atgttgact tttggcatc 
atttacacc gttttctta tttggtttt tcgcagtcg 
ttctatttc ctgtataag ggattcccc ggggtcaaa 
acttcctta tacaacacg tacatgtcg acttggatg 
gccttattc caaattact ttgggcaat tatgaatat 
tctgagttg agtgccacc acataccca gcggtcagc 
aaaacagtc tttgccatc ttcatggtg ttcgtccca 
attttgctg ctcaacatg ttaatcgcc atgatgggc 
aacacttac gcccatgta atcgagcaa agcgagaaa 
gaatgggtc aaacaatgg gcaaaaatc gtcatcgcc 
ctggaacgg gcaatccca caatccgac gcccaacat 
tacttacaa gagtacagt atttcgctg gggcccagt 
gaacaggac cccagtacc gaaaaacgc ggagttttg 
gtcataaaa tcaaagagt aaaacgcgg gcaaaacaa 
cgaaaaggg gccgtggcc aattggaag cgggtgggc 
aaagtaacg ataaacgcg ctcaaaaaa cgcggctta 
accggtgaa gaaatgcgc tgcttgatg tggggccgc 
gagtctatt aatacaccg gtcaaaaca aagaagcct 
gtgaaggac ccactgctg gacccgcaa gggccgaat 
ttaacgggt ggcttcggc gatgcttta accactgct 
ttggatgtg atgacgttc acgcacgat ttggatatt 
gtgggggct tcccagggc ctgaatttg gccacgaac 
cctaaaccc gtccctccc acaacaaca gcgagtgcc 
gtgactgtg aataaccag ataaattcg gctctgaac 
gcgcagcaa aaggccgta gctggggct ggagttggt 
gctttggct atgataggc acagtgacc caactcacc 
gactctcaa ggctacatt atgcaaaac agtgtgaaa 
aaagaagaa aaaattgtc gcaactttg gaggaccct 
ttcagggaa cttgtcata aacgctaat gacagtaac 
tgcgacccg gaaaaatta aaaatgctt gcgctatcg 
gccgccaat ttgaaagac gttgaagag ttatcggtg 
gccaagcca caaacgaaa agtgttaag agtctagcg 
gggattttc gccgggact gagactttt gtgaggaaa 
gttgaagaa acaataaag aagaaatat gcggctttg 
gatccaagt gatagtgaa ggttttggc gaacctcca 
ctattaggg aaaatatcc aggactaga agggccaaa 
tcggcaaac ttgcgcaac tcttcagcg cgatctaaa 
gcttcagat aagaaaaaa ctggtcgcg gggtcacag 
tcaagctcc actgatacg gtcaataac gaaattaat 
gaaaagaat atcgaaaat tctgactta gactatgcc 
gaagagagg atcaagctg gtgaaagag tcgttaaag 
caggtggtt gacgttgcg caaattcgg ccgattaac 
atcgatgtg gccctcgag gagcaagtg tcggtcaca 
atttcggaa acgatgcga gtgcagggc tccggggac 
ggggccgaa gtccagtca tccaacgtc aaaccaaag 
cgcaaaaaa cggagcaaa acagccaaa aacaacaag 
gttgcgccc gcggttgaa ccgagacct tgtcggtca 
gctaacgac cacttcgat tccgacgat tctgaatca 
cctgaccct ttagaacct tggagtaca aaaaaaatc 
gcaaatatg aacaaaatt ctagcttgg gagaacgac 
caagacagc atgtaatct ttcaaacct gtcaaataa 
attctattt ttttgc