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Gene information
General information
TC012367 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5766059..5782492
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012367
    iBeetle screen iB_02913

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 3
    vitellogenic egg chamber structure irregular - Number of animals showing the phenotype: 4
    vitellogenic egg chamber potentially partiallydegenerated - Number of animals showing the phenotype: 4

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: Penetrance: about 50% The muscle bundles seem to be thinner and more fragile than usual
    larval musculature partially thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Cuticles look wt. Many old (L1-L2) larvea which died potentially during or just before molting.

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Pupal morphology
    wings slightly shortened - Number of animals showing the phenotype: 5

    oblique dorsal thoracic muscles irregular - Number of animals showing the phenotype: 4
    oblique dorsal thoracic muscles not present - Number of animals showing the phenotype: 2

    oblique dorsal thoracic muscles thinner - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012367 RA
      MVVGDPSIN NIMSGIENA GGVKQHNHK KKLKQRFDI 
      IKKLGQGTF GKVQLGINK ETGQEVAIK TIKKSKIES 
      EADLVRIRR EIQIMSSVQ HPNIIHIYE VFENREKMV 
      LVMEYAAGG ELYDYLSER KILDENEAR RIFRQIATA 
      CYYCHKHKI CHRDLKLEN ILLDENNNA KIADFGLSN 
      VFDDQRLLS TFCGSPLYA SPEIVKGTP YHGPEVDCW 
      SLGVLLYTL VYGAMPFDG SNFKRLVRQ ISQGDYFEP 
      AKPSPASPL IREMLTVNP KNRADIEKI CTHWWVNEN 
      YDVSCLEIS EELANQTPV RLDLLLSLA PPPPQLESD 
      KLVVTGDVA EEMKTEVAP PTRSQSVGS LMELTHPAE 
      RRIKELLTE EKASPKRKL ENTVSTDRV KDGLKRKDK 
      IIKENTVAD ITLHGAIKE NASLEDASM SEPIPLDKS 
      LTQTITRDM DVETNEEPV DPSLQGAAC SEIIEEAKK 
      TTEKKPKTK KTLSTSVSN KALEGINEI PSQENVNNV 
      DKENKDVVA QPEKTEKPE SKIAKKKTV KKKVLTDKN 
      ETVKPKEPS PPPAPPVEE KPKEPEEKP TKPIERRKS 
      RIFEAAEKF QNMISSPTE SKPTPVEKP KKIVIPGVS 
      VDGFKKEFE RKASLTSTS PPKLKGTPS KKILIDQQK 
      PPEADKEEP KPDPETPKP VIDDERKER VRNAVSIIS 
      SALDKEGAR KSKSRPCCV RKPPVPFGV GGRSASGNI 
      GLLASPLSP PLGPKPFVR PQFDSKAEV KKVEEKPEV 
      EEENKTSSA EITLKSATL PRRKTTKAE IQLNYPMPK 
      PATMEFKTE MAHNVTAPP KLATQRSEV VVPVTAPSV 
      GNLRSSSLE PESKGSPKE RIIPISFEQ QDTKEAVQS 
      PPTKPPMPR PLQSQKSTT SQRSNSLSR QSTQDSDTE 
      TTSGEPIKK SPREYIIPI AVEGGGYVT PRAGSVEPS 
      DTASTTSTM TNKSRNKFG KARRLNSLF SDRDSEDET 
      SFPSLYRHS SFGKDSDNE DSRKDAFHL HRLRSTRPK 
      RTILEHNDS LSSGEEDDD DGFEILTAE HLFSNLLSR 
      VRDLTQKLN VEDGGRPGF PSSRLLSHF DHGTNFWNF 
      HNRLDPLSR HSTLGRSLS RERSNETSA TPWRRSVSR 
      DLASDIESV FKDPNSTAT RPRDKKAKK ENLNLADLD 
      LKKIKLSDE DALALSYLT PGLSRRIQK HLLSQLAPS 
      EAQKLQRTL SSNGSNNYE DNHKFARRS VDRLSSTLP 
      HRYSVENNL NEPNYKSGL PVKLRDDNR SASVAYDND 
      FGYKRPKAV RSVSLREPK NYLPNKDKS PDSSLSESL 
      SRSDVTDSY TTSLSRPSS YSKYSDLSN KYLTPKTEI 
      KKPSSTPRR ISRFLRPDF FDPPKEENV LVKEKKERE 
      METQKVLKE IRDKRKCRL NARRERSAS REKSTDFED 
      EKKDSNKLS TPDEGPKET NRLLSKVSN NIKNLENNV 
      KTLHDYVNV TVPKDNKEI EGNKGVHDY VNVKVTKPE 
      EPPVVKKTR ISKLVRPKS YPTESVIEK EKEKKVSPE 
      KESKISRLR KGFTRQGSK ERDKTKEDK NETNKSINE 
      EEKGHKNKL LQSIEKKLE KFRSSSKDP EEAKALKEK 
      KSGVESAIK RLREQSLPR NLDHCTESG LIKRAVSVE 
      DVSNLSKPL QASRKSVTK ILGLFKKYE EQENKKNDK 
      IVKKKKFKE SPNRDEAVI ETSQEPKKP ERPRSLLLD 
      KMKQFQPPD NKASKASRL PVNSFRRSL NLEVDVPKK 
      TEAKRNSEP RSAAEESRE NRNSVATDD SSALLSPDD 
      NVSDSWSVC SDYHNQDLH SPVSPNGVL CSGDESESV 
      IDRIRRKSF YTRFNEKKR TRKPSLTRN YKDLDLYKN 
      TDYGSLDRR SSYDYKAPT RRSSYVSTL QDPVKRDYK 
      NYARSSSLL NDYVNVPSR YQTYNARVS RPTTVFSDD 
      ETTLDELLS AAKSRKYST PVPSINRGS VSPVNGPTS 
      SVNSPEPV
    CDS sequence
    • >TC012367 RA
      atggttgtc ggagacccg agtataaat aatataatg 
      agcggcatt gagaatgcc ggcggagtt aaacaacac 
      aatcacaaa aagaaattg aaacagagg ttcgatata 
      ataaagaaa ctgggtcaa ggcacgttc ggcaaagtg 
      caattagga ataaacaaa gagactggt caagaagta 
      gcaataaaa acaatcaaa aaatccaaa atcgaatcg 
      gaggccgac ctagtccgg ataaggcgc gaaattcaa 
      atcatgtct tcggtgcaa catcctaat attatccac 
      atttacgaa gtgtttgaa aatagggaa aaaatggtg 
      ctagtgatg gaatatgcg gcagggggc gagctttac 
      gattatttg tcggagcgc aagatttta gacgagaac 
      gaagccagg cgaatattt cgacagatc gccactgcg 
      tgttactat tgccacaaa cataaaatt tgccatagg 
      gatttgaaa ctggaaaat attcttttg gacgaaaac 
      aataatgct aagattgcc gatttcgga ctgtcaaat 
      gtttttgac gatcaaaga ctgttaagc actttctgt 
      ggttcccct ctatacgct tctcccgaa attgtcaaa 
      gggacgccc taccatgga ccagaagtc gactgttgg 
      tcgttaggt gtactttta tatactcta gtctacggt 
      gccatgcct tttgatgga agcaatttt aaacgttta 
      gttaggcaa atttcgcaa ggagattac ttcgagcca 
      gccaaaccc agtcccgcc tctcctctc attagggaa 
      atgttaacc gtgaatcct aaaaataga gcagatata 
      gaaaaaatt tgtacccac tggtgggtg aacgagaat 
      tacgacgtt agctgttta gagatctca gaagaatta 
      gccaatcaa actcccgta agattagat ttgttatta 
      tcattggct cctccgcca cctcagctt gaaagtgac 
      aaacttgtg gttactgga gatgttgct gaagagatg 
      aagactgaa gttgcaccg cccacacga tcccaatct 
      gtaggtagt ttaatggaa ttaactcat cccgctgaa 
      cgaaggatc aaagaactt ttaaccgaa gaaaaagct 
      tcacctaaa cgtaaacta gaaaatact gttagtacg 
      gatcgagtc aaggatggc cttaagaga aaggataag 
      ataatcaaa gagaatact gttgctgat attaccctc 
      cacggtgca attaaggaa aatgcttct ttggaagat 
      gcaagtatg tctgagcca atcccattg gataagagt 
      ctaactcaa acaataaca cgtgacatg gacgttgaa 
      acaaacgaa gaaccagtt gatccgtcg ttacaagga 
      gccgcttgt agtgagata atcgaagag gcaaagaaa 
      acaacggaa aagaagccc aaaacaaag aaaactcta 
      tcaactagt gtcagcaat aaagcattg gaaggcata 
      aatgaaatt cctagtcaa gaaaacgta aacaatgta 
      gataaagaa aacaaggac gttgttgct caacctgaa 
      aaaaccgag aaaccggaa tccaaaatc gcgaagaag 
      aaaacagtc aagaagaag gtcttaacc gataagaac 
      gaaacagtt aagcccaaa gaaccatcg ccaccccca 
      gccccaccc gtcgaagaa aaaccaaaa gaacccgaa 
      gaaaaaccc accaaacca atcgaacgg agaaaatcg 
      cgtattttc gaagcggcc gaaaaattc caaaacatg 
      atttcatcc cccaccgag tccaaaccc acccccgtg 
      gaaaagcca aagaaaatc gtaatcccc ggcgtcagc 
      gttgacggt ttcaagaag gagtttgaa cgcaaagca 
      agcctcaca tcaacatct cccccaaaa ctgaaaggc 
      accccctcg aagaaaatc ctcatcgat caacaaaaa 
      ccccccgaa gctgacaag gaggagccc aaaccagac 
      cccgaaaca ccaaagcct gtgatcgac gatgagcgc 
      aaagagcgc gtgcgcaat gccgttagt attatctca 
      agtgcctta gacaaggaa ggagcgcgg aaatcaaaa 
      tctcggccg tgttgcgtc cggaagccc ccggtgccg 
      ttcggggtt ggcggccgc tcggcttcg ggcaacatc 
      gggctgctg gcgtccccc ctgtcgccc ccattgggc 
      cccaagccg ttcgtccgg ccccagttt gatagcaaa 
      gccgaggtg aagaaggtg gaggagaag ccggaggtt 
      gaagaggag aataagacc tcctcggcg gagattacg 
      ctcaaatcg gccacgttg ccgaggagg aagaccacc 
      aaagcggaa attcagctc aattatccc atgcctaag 
      ccagctacc atggagttt aagactgaa atggctcat 
      aatgtgacg gcgccgccc aagttggcg acgcagagg 
      agtgaggtt gtggtgccg gtgacggcg ccatctgtc 
      gggaactta agatcatca tcgctggaa cccgaatcg 
      aagggctcc cccaaagag cgaatcatc ccgatttcc 
      ttcgaacag caagacaca aaggaggcc gtccaatcg 
      ccccctacc aaaccacca atgccgcga cctctccaa 
      agccaaaaa tcgacaaca tcgcaaaga tccaacagc 
      ttgtcacga cagtccact caagactcc gacactgaa 
      acaacatcc ggcgaaccg attaaaaaa tccccgaga 
      gaatacatc atcccgatt gcggtcgaa ggcggtggt 
      tatgtgacc ccacgcgct ggtagcgtg gaaccgagt 
      gacacagcg agtacaaca agcaccatg acaaacaaa 
      tcgaggaac aagtttggc aaagccagg agattaaac 
      tccttgttt agtgatcgg gactcggaa gacgagacc 
      tccttcccc agcttgtat cgccacagt tcgttcggg 
      aaagacagc gacaatgag gattcgcga aaggatgcg 
      ttccatttg cacaggctg aggagtaca agaccgaag 
      aggaccatt ttggagcac aacgattcg ctcagttct 
      ggcgaggag gacgacgat gacgggttt gagatttta 
      acagccgaa cacttattc tccaatttg ttatcaagg 
      gtaagggat ttgacccag aaattgaat gttgaagat 
      gggggcagg cctggattc cccagcagt cggttattg 
      agccatttt gaccatggc accaacttc tggaatttt 
      cacaaccgg ctagacccc ttgtccagg cattcaacg 
      ttgggccgg agcctgagc cgcgaacgc agcaacgag 
      acctcagct acgccttgg cgccgtagc gtgagtcgt 
      gacttagcc tcagatatc gaaagtgtc ttcaaagac 
      ccaaactcg acagcgacg cgcccccga gataaaaaa 
      gcgaagaaa gaaaacctt aatttagca gatttagat 
      cttaaaaaa attaaatta agcgatgaa gacgcttta 
      gctttgtcg tacctaaca cctggcttg tcacgacgg 
      attcaaaaa cacttactg tcacaactc gcgccatct 
      gaagcccaa aaactgcaa cggaccctg tcctcaaac 
      ggaagtaac aactacgaa gacaaccac aaatttgca 
      cgccgatca gttgaccga ttgtcttca acattacct 
      catcgctac tctgtagaa aataactta aacgaaccg 
      aactacaaa tcgggcctt ccggtgaag cttcgcgat 
      gacaacaga tcggctagt gtggcctat gataatgac 
      tttggctac aaaagaccc aaagcggtt cgttccgtc 
      tcactcaga gagcccaaa aactatttg ccaaacaaa 
      gacaaaagt cccgattcg agcctttcg gaaagcttg 
      tcacgaagc gacgtaacc gacagctac accacgtcc 
      ctatcacgt ccttcctcc tactcgaaa tattcagac 
      ttgtcgaat aaatactta acaccaaag actgaaatt 
      aaaaaaccg tcctcaacg cctcgaaga atctcgagg 
      tttttacga cctgatttc ttcgaccca cccaaagaa 
      gaaaacgtg ctagtcaag gaaaagaaa gagcgggaa 
      atggaaacg caaaaggtt ttgaaagag attcgggac 
      aaaagaaag tgtcgactg aatgccagg cgggaaagg 
      tccgcctca cgtgagaaa agcaccgat ttcgaggac 
      gagaaaaag gattcgaac aaattgtca actcctgac 
      gaaggtccg aaagagacc aaccgctta ttgagcaaa 
      gtttcgaac aacattaaa aaccttgaa aataacgtc 
      aagacgctc catgactac gtcaatgta acggtcccg 
      aaagacaac aaagaaatc gaaggaaat aaaggagtt 
      catgattat gtcaacgtt aaagtcaca aagcctgaa 
      gagcccccc gttgtgaaa aaaacccga atttcgaaa 
      cttgtccgc ccaaaaagc tacccgacc gagagtgtt 
      attgaaaaa gagaaagag aagaaggtt tcaccggaa 
      aaagaatca aaaattagc cggttacgt aaaggtttc 
      acgcgtcaa ggctcaaaa gaacgggac aaaacaaaa 
      gaagataaa aacgagact aacaaaagc ataaatgag 
      gaagaaaaa ggccacaag aataagctg cttcagtcg 
      attgaaaag aaactggag aagttccgc tcgtcgtcg 
      aaagacccc gaggaggcc aaagccctg aaggagaaa 
      aaatccggc gttgagagc gccatcaag cggctccgg 
      gagcaaagt ctcccccgg aatttggac cactgcacg 
      gagtctggc cttattaaa agggccgtt tcggtcgaa 
      gacgtgtca aatttgagt aagccgttg caagcgtcg 
      cgcaaaagt gtgacaaaa attttgggt ttgttcaaa 
      aaatacgag gaacaagag aacaaaaag aacgacaaa 
      atcgtgaaa aagaaaaag ttcaaggag agtccgaat 
      cgtgacgaa gctgtgatt gaaactagt caagagccg 
      aaaaagccc gaacgtccc cggtccctc ctcctggac 
      aaaatgaag cagtttcaa ccccctgac aacaaagct 
      agcaaagcg tcgcgcttg ccagtcaat tcatttaga 
      cgtagtttg aatttagaa gtcgatgtt cctaaaaaa 
      accgaagcg aaacgcaac agcgaacct cgcagtgca 
      gctgaagag agtcgggag aatcgcaat tcggtggcg 
      accgacgat agttcggcg ctgttgtcg ccggatgat 
      aacgtgagc gactcgtgg tcggtgtgc tccgactac 
      cacaaccag gacttgcac tcgccggtg tcacccaat 
      ggggttttg tgctccggc gacgagagc gagtcggtg 
      attgaccgg atccgacgc aagagtttc tatacgagg 
      ttcaacgag aagaaacga actcgcaag ccgagtctg 
      acccggaat tacaaagat ctcgacttg tacaaaaac 
      acggattat gggtctttg gaccgtaga tcgagttat 
      gactataaa gcgccaact cgtcgatcg tcgtacgtg 
      tcgactctc caggatccg gtcaagcgg gattataag 
      aattatgct cgaagttcg tcgcttttg aacgactat 
      gtcaacgtc ccgagtcgc taccagact tacaacgcg 
      agggtaagt cggccaact acggttttc tccgacgac 
      gaaacgact ttggatgag ctgctttca gctgcgaaa 
      tctcgaaag tacagtacc ccggttcca tccataaat 
      agaggctct gtttctccc gttaatgga cctacgtct 
      tccgttaat tcccctgaa cctgtttaa
    mRNA sequence
    • >TC012367 RA
      tggttataa aaacgcacc aatgtttaa caaacattt 
      gaatatttt ccatgtttt ggtcgtttt tttcaaagt 
      gggacgagt tcgcgccaa aattcaaac gcaacattc 
      caatgtcgt gtagggacc tatttttaa accgaagaa 
      acatggcgg cgagcagac atatcgggc tatttaaaa 
      tgttaaaaa aactctttt tccattgat aataataat 
      gtgaatgcg aaattcgtg cgcctccaa gtgctgcga 
      accgaaaaa accgtgttg tgcgtaaat tcgagtgaa 
      atccggcga ttgtggtgc ccgctcttg gcaatttca 
      aatctacgg ggtgtttcg atgaattaa ggcggcaaa 
      atggttgtc ggagacccg agtataaat aatataatg 
      agcggcatt gagaatgcc ggcggagtt aaacaacac 
      aatcacaaa aagaaattg aaacagagg ttcgatata 
      ataaagaaa ctgggtcaa ggcacgttc ggcaaagtg 
      caattagga ataaacaaa gagactggt caagaagta 
      gcaataaaa acaatcaaa aaatccaaa atcgaatcg 
      gaggccgac ctagtccgg ataaggcgc gaaattcaa 
      atcatgtct tcggtgcaa catcctaat attatccac 
      atttacgaa gtgtttgaa aatagggaa aaaatggtg 
      ctagtgatg gaatatgcg gcagggggc gagctttac 
      gattatttg tcggagcgc aagatttta gacgagaac 
      gaagccagg cgaatattt cgacagatc gccactgcg 
      tgttactat tgccacaaa cataaaatt tgccatagg 
      gatttgaaa ctggaaaat attcttttg gacgaaaac 
      aataatgct aagattgcc gatttcgga ctgtcaaat 
      gtttttgac gatcaaaga ctgttaagc actttctgt 
      ggttcccct ctatacgct tctcccgaa attgtcaaa 
      gggacgccc taccatgga ccagaagtc gactgttgg 
      tcgttaggt gtactttta tatactcta gtctacggt 
      gccatgcct tttgatgga agcaatttt aaacgttta 
      gttaggcaa atttcgcaa ggagattac ttcgagcca 
      gccaaaccc agtcccgcc tctcctctc attagggaa 
      atgttaacc gtgaatcct aaaaataga gcagatata 
      gaaaaaatt tgtacccac tggtgggtg aacgagaat 
      tacgacgtt agctgttta gagatctca gaagaatta 
      gccaatcaa actcccgta agattagat ttgttatta 
      tcattggct cctccgcca cctcagctt gaaagtgac 
      aaacttgtg gttactgga gatgttgct gaagagatg 
      aagactgaa gttgcaccg cccacacga tcccaatct 
      gtaggtagt ttaatggaa ttaactcat cccgctgaa 
      cgaaggatc aaagaactt ttaaccgaa gaaaaagct 
      tcacctaaa cgtaaacta gaaaatact gttagtacg 
      gatcgagtc aaggatggc cttaagaga aaggataag 
      ataatcaaa gagaatact gttgctgat attaccctc 
      cacggtgca attaaggaa aatgcttct ttggaagat 
      gcaagtatg tctgagcca atcccattg gataagagt 
      ctaactcaa acaataaca cgtgacatg gacgttgaa 
      acaaacgaa gaaccagtt gatccgtcg ttacaagga 
      gccgcttgt agtgagata atcgaagag gcaaagaaa 
      acaacggaa aagaagccc aaaacaaag aaaactcta 
      tcaactagt gtcagcaat aaagcattg gaaggcata 
      aatgaaatt cctagtcaa gaaaacgta aacaatgta 
      gataaagaa aacaaggac gttgttgct caacctgaa 
      aaaaccgag aaaccggaa tccaaaatc gcgaagaag 
      aaaacagtc aagaagaag gtcttaacc gataagaac 
      gaaacagtt aagcccaaa gaaccatcg ccaccccca 
      gccccaccc gtcgaagaa aaaccaaaa gaacccgaa 
      gaaaaaccc accaaacca atcgaacgg agaaaatcg 
      cgtattttc gaagcggcc gaaaaattc caaaacatg 
      atttcatcc cccaccgag tccaaaccc acccccgtg 
      gaaaagcca aagaaaatc gtaatcccc ggcgtcagc 
      gttgacggt ttcaagaag gagtttgaa cgcaaagca 
      agcctcaca tcaacatct cccccaaaa ctgaaaggc 
      accccctcg aagaaaatc ctcatcgat caacaaaaa 
      ccccccgaa gctgacaag gaggagccc aaaccagac 
      cccgaaaca ccaaagcct gtgatcgac gatgagcgc 
      aaagagcgc gtgcgcaat gccgttagt attatctca 
      agtgcctta gacaaggaa ggagcgcgg aaatcaaaa 
      tctcggccg tgttgcgtc cggaagccc ccggtgccg 
      ttcggggtt ggcggccgc tcggcttcg ggcaacatc 
      gggctgctg gcgtccccc ctgtcgccc ccattgggc 
      cccaagccg ttcgtccgg ccccagttt gatagcaaa 
      gccgaggtg aagaaggtg gaggagaag ccggaggtt 
      gaagaggag aataagacc tcctcggcg gagattacg 
      ctcaaatcg gccacgttg ccgaggagg aagaccacc 
      aaagcggaa attcagctc aattatccc atgcctaag 
      ccagctacc atggagttt aagactgaa atggctcat 
      aatgtgacg gcgccgccc aagttggcg acgcagagg 
      agtgaggtt gtggtgccg gtgacggcg ccatctgtc 
      gggaactta agatcatca tcgctggaa cccgaatcg 
      aagggctcc cccaaagag cgaatcatc ccgatttcc 
      ttcgaacag caagacaca aaggaggcc gtccaatcg 
      ccccctacc aaaccacca atgccgcga cctctccaa 
      agccaaaaa tcgacaaca tcgcaaaga tccaacagc 
      ttgtcacga cagtccact caagactcc gacactgaa 
      acaacatcc ggcgaaccg attaaaaaa tccccgaga 
      gaatacatc atcccgatt gcggtcgaa ggcggtggt 
      tatgtgacc ccacgcgct ggtagcgtg gaaccgagt 
      gacacagcg agtacaaca agcaccatg acaaacaaa 
      tcgaggaac aagtttggc aaagccagg agattaaac 
      tccttgttt agtgatcgg gactcggaa gacgagacc 
      tccttcccc agcttgtat cgccacagt tcgttcggg 
      aaagacagc gacaatgag gattcgcga aaggatgcg 
      ttccatttg cacaggctg aggagtaca agaccgaag 
      aggaccatt ttggagcac aacgattcg ctcagttct 
      ggcgaggag gacgacgat gacgggttt gagatttta 
      acagccgaa cacttattc tccaatttg ttatcaagg 
      gtaagggat ttgacccag aaattgaat gttgaagat 
      gggggcagg cctggattc cccagcagt cggttattg 
      agccatttt gaccatggc accaacttc tggaatttt 
      cacaaccgg ctagacccc ttgtccagg cattcaacg 
      ttgggccgg agcctgagc cgcgaacgc agcaacgag 
      acctcagct acgccttgg cgccgtagc gtgagtcgt 
      gacttagcc tcagatatc gaaagtgtc ttcaaagac 
      ccaaactcg acagcgacg cgcccccga gataaaaaa 
      gcgaagaaa gaaaacctt aatttagca gatttagat 
      cttaaaaaa attaaatta agcgatgaa gacgcttta 
      gctttgtcg tacctaaca cctggcttg tcacgacgg 
      attcaaaaa cacttactg tcacaactc gcgccatct 
      gaagcccaa aaactgcaa cggaccctg tcctcaaac 
      ggaagtaac aactacgaa gacaaccac aaatttgca 
      cgccgatca gttgaccga ttgtcttca acattacct 
      catcgctac tctgtagaa aataactta aacgaaccg 
      aactacaaa tcgggcctt ccggtgaag cttcgcgat 
      gacaacaga tcggctagt gtggcctat gataatgac 
      tttggctac aaaagaccc aaagcggtt cgttccgtc 
      tcactcaga gagcccaaa aactatttg ccaaacaaa 
      gacaaaagt cccgattcg agcctttcg gaaagcttg 
      tcacgaagc gacgtaacc gacagctac accacgtcc 
      ctatcacgt ccttcctcc tactcgaaa tattcagac 
      ttgtcgaat aaatactta acaccaaag actgaaatt 
      aaaaaaccg tcctcaacg cctcgaaga atctcgagg 
      tttttacga cctgatttc ttcgaccca cccaaagaa 
      gaaaacgtg ctagtcaag gaaaagaaa gagcgggaa 
      atggaaacg caaaaggtt ttgaaagag attcgggac 
      aaaagaaag tgtcgactg aatgccagg cgggaaagg 
      tccgcctca cgtgagaaa agcaccgat ttcgaggac 
      gagaaaaag gattcgaac aaattgtca actcctgac 
      gaaggtccg aaagagacc aaccgctta ttgagcaaa 
      gtttcgaac aacattaaa aaccttgaa aataacgtc 
      aagacgctc catgactac gtcaatgta acggtcccg 
      aaagacaac aaagaaatc gaaggaaat aaaggagtt 
      catgattat gtcaacgtt aaagtcaca aagcctgaa 
      gagcccccc gttgtgaaa aaaacccga atttcgaaa 
      cttgtccgc ccaaaaagc tacccgacc gagagtgtt 
      attgaaaaa gagaaagag aagaaggtt tcaccggaa 
      aaagaatca aaaattagc cggttacgt aaaggtttc 
      acgcgtcaa ggctcaaaa gaacgggac aaaacaaaa 
      gaagataaa aacgagact aacaaaagc ataaatgag 
      gaagaaaaa ggccacaag aataagctg cttcagtcg 
      attgaaaag aaactggag aagttccgc tcgtcgtcg 
      aaagacccc gaggaggcc aaagccctg aaggagaaa 
      aaatccggc gttgagagc gccatcaag cggctccgg 
      gagcaaagt ctcccccgg aatttggac cactgcacg 
      gagtctggc cttattaaa agggccgtt tcggtcgaa 
      gacgtgtca aatttgagt aagccgttg caagcgtcg 
      cgcaaaagt gtgacaaaa attttgggt ttgttcaaa 
      aaatacgag gaacaagag aacaaaaag aacgacaaa 
      atcgtgaaa aagaaaaag ttcaaggag agtccgaat 
      cgtgacgaa gctgtgatt gaaactagt caagagccg 
      aaaaagccc gaacgtccc cggtccctc ctcctggac 
      aaaatgaag cagtttcaa ccccctgac aacaaagct 
      agcaaagcg tcgcgcttg ccagtcaat tcatttaga 
      cgtagtttg aatttagaa gtcgatgtt cctaaaaaa 
      accgaagcg aaacgcaac agcgaacct cgcagtgca 
      gctgaagag agtcgggag aatcgcaat tcggtggcg 
      accgacgat agttcggcg ctgttgtcg ccggatgat 
      aacgtgagc gactcgtgg tcggtgtgc tccgactac 
      cacaaccag gacttgcac tcgccggtg tcacccaat 
      ggggttttg tgctccggc gacgagagc gagtcggtg 
      attgaccgg atccgacgc aagagtttc tatacgagg 
      ttcaacgag aagaaacga actcgcaag ccgagtctg 
      acccggaat tacaaagat ctcgacttg tacaaaaac 
      acggattat gggtctttg gaccgtaga tcgagttat 
      gactataaa gcgccaact cgtcgatcg tcgtacgtg 
      tcgactctc caggatccg gtcaagcgg gattataag 
      aattatgct cgaagttcg tcgcttttg aacgactat 
      gtcaacgtc ccgagtcgc taccagact tacaacgcg 
      agggtaagt cggccaact acggttttc tccgacgac 
      gaaacgact ttggatgag ctgctttca gctgcgaaa 
      tctcgaaag tacagtacc ccggttcca tccataaat 
      agaggctct gtttctccc gttaatgga cctacgtct 
      tccgttaat tcccctgaa cctgtttaa attatttct 
      ctcaaacaa tgtgatcac tagtataca aaacgcaga 
      ctcaaagat tcatgttag agttagagc gtagtcata 
      tcaattttt ctgttaaat taaggtttt taactaacg 
      aaagtattg cagtcttat ttatttatt tactagaga 
      aatgtagat acataaaag tgtattatt tatatgtaa 
      atgttgttt gctttttta tttattgtt agaagatta 
      gttcacttc gagtttttc tagtaggta ttttgtaac 
      ttattattt aacaaaaat tggattgaa agcgttata 
      aaacactat tgtagcaat tgttcaagt ttacataat 
      tttaattca gttgactct ctaagacaa atgcataag 
      attaagttg agttcgtgt gaattgtta gaattgtat 
      gttgaattt ggtgatttt ataaaaaat atatttcaa 
      aattaact
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