Status
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Gene information
General information
TC034287 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 2324196..2361714
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034287
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02911

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    egg chamber potentiallynumber increased - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034287 RA
      MVLRCLVAL VVLRVVQCG ILDWARSDK IEVNLPWLP 
      FENETRCYD DLGCLNITR SWYHLILRP INLFPLPRN 
      VINTRFILY TRFNPNEGT YLKVEDEST IEESNFNPN 
      KSTKFIVHG FIDTPLSNW VRDMKDELI KAGDMNVIV 
      VDWAGGSLP LYTQATANT RLVGMEIAH LIKFLMEKY 
      DIDASDFHI IGHSLGAHT AGYAGSLVP KLGRITGLD 
      PAEPFFQGM PPHVRLDPS DAEFVDVIH TDGKGIIFL 
      GYGMSQPCG HLDFYPNNG KEQPGCDIT QTPLVPLTL 
      IRDGLEEAS RVLVACNHV RAIKLFIDS INTQCPYIG 
      HQCSSFEQF MAGKCFTCK PGTTNCAVM GYHAHTSPG 
      IENNEISQS KLTDLVGNK YFLTTGREF PFCRRLYRV 
      RIELAKPRM AESWVQGFI KASIYSSAG VIHVDLTPS 
      GETKLEHGT SFTSVVSHP ADLGGNIKK VELSWEYEL 
      NVLEPKTIC LFWCNDHLY VESVAVDEM ALPGRGKRD 
      ASFSNKLCP KGKKSYSDI ASRGKGVFV DLCKQSDKY 
      DKS
    CDS sequence
    • >TC034287 RA
      atggtactg aggtgtttg gtggcactg gtcgtgttg 
      cgcgtggtt cagtgcggg attttggac tgggcaagg 
      tccgataaa atcgaagtt aatttgcct tggttgcct 
      ttcgagaat gagaccagg tgttatgat gacttggga 
      tgtttgaat attacaagg agttggtat catttgata 
      ctcaggccc attaatttg tttccgttg cccaggaat 
      gtcatcaat acgaggttt atactctac acacgcttc 
      aatccaaat gaggggacc tacctcaaa gtggaagat 
      gaaagcacc atcgaagaa tcaaatttt aacccgaac 
      aaaagtaca aaattcata gtacacggt tttatcgac 
      accccttta tcaaactgg gtgagagat atgaaagat 
      gagctgatc aaagcaggt gatatgaat gtgattgtg 
      gtggattgg gctgggggt agtttgccc ctttacaca 
      caagctaca gccaatacg cggctagta ggtatggaa 
      attgcccat ttaatcaaa tttttgatg gaaaaatat 
      gatattgat gccagcgat ttccacatt attgggcac 
      tctttaggg gcacacact gctggatat gcaggttcg 
      cttgtgccc aagctgggg aggatcact ggactggat 
      cctgccgag ccgtttttt caggggatg cctccccat 
      gtccgtctg gacccttcc gacgccgaa ttcgtcgac 
      gtgatccac accgacggc aaaggcatc atcttcctc 
      ggctacgga atgtcgcag ccttgcggc catttagac 
      ttctatccc aacaatggg aaagagcaa cctggatgt 
      gatattacc caaactcct ttagtacct ctgaccctc 
      atcagggat ggactagag gaagcgtcc agagttctg 
      gtcgcttgt aatcatgta agagccata aaattattt 
      attgatagt ataaacaca cagtgccct tacataggg 
      catcaatgt tccagtttc gagcagttt atggctggg 
      aaatgcttc acttgtaag cctggaact acaaattgc 
      gctgtgatg ggttatcat gcccacact agcccaggg 
      attgaaaat aatgaaatc tcccaatcg aaactgacc 
      gatttggtc gggaataaa tatttcctt accacggga 
      cgagagttc ccgttttgc cgaagactg taccgagtg 
      agaatagaa ttagctaaa ccccgaatg gccgaatcc 
      tgggtccag ggtttcatc aaagcctcc atttacagt 
      tccgctggt gtaatccac gtggattta acaccctct 
      ggtgaaacc aaactggaa cacggcacc agtttcacc 
      agtgtcgtc tcacatcca gccgactta ggtggcaat 
      ataaaaaaa gtcgaactg agttgggag tacgaacta 
      aatgttctc gaaccaaaa actatttgt ctgttttgg 
      tgcaacgat cacttgtat gttgaaagt gtagccgtg 
      gatgagatg gcattgccg ggaagaggg aaaagagat 
      gcgtcgttt tcgaacaag ctttgtccc aaaggaaag 
      aagagttac tcggatata gcaagcagg gggaaagga 
      gtgttcgtt gatttgtgc aaacagtca gataaatac 
      gataagagc tga
    mRNA sequence
    • >TC034287 RA
      ctctaatta aatacctcc aacttcctg caaacgcat 
      cttcaccca actccaagc aatcgttgc aaaatgacg 
      caactttga tgggattag aaagtaacc aagtcgtgg 
      agcgatttt tatgcggcg cgctccagt ggtggagaa 
      agtaaaatg ttttcaaca ataataagt gtctgtcgg 
      tgcagtgtg agtgcgcct tcggtgctg tttagtcgt 
      tggagtgct tcgttacgt aattatttt ctacagggg 
      attcacgtg gtcgagtgc agagcgagc aagggaagc 
      cttttggaa tagtattca gtgtgtgag tgcctccaa 
      ctccagatc catgcaaag gctacaacc gcccaagaa 
      acggttagt accgggaag atggtactg aggtgtttg 
      gtggcactg gtcgtgttg cgcgtggtt cagtgcggg 
      attttggac tgggcaagg tccgataaa atcgaagtt 
      aatttgcct tggttgcct ttcgagaat gagaccagg 
      tgttatgat gacttggga tgtttgaat attacaagg 
      agttggtat catttgata ctcaggccc attaatttg 
      tttccgttg cccaggaat gtcatcaat acgaggttt 
      atactctac acacgcttc aatccaaat gaggggacc 
      tacctcaaa gtggaagat gaaagcacc atcgaagaa 
      tcaaatttt aacccgaac aaaagtaca aaattcata 
      gtacacggt tttatcgac accccttta tcaaactgg 
      gtgagagat atgaaagat gagctgatc aaagcaggt 
      gatatgaat gtgattgtg gtggattgg gctgggggt 
      agtttgccc ctttacaca caagctaca gccaatacg 
      cggctagta ggtatggaa attgcccat ttaatcaaa 
      tttttgatg gaaaaatat gatattgat gccagcgat 
      ttccacatt attgggcac tctttaggg gcacacact 
      gctggatat gcaggttcg cttgtgccc aagctgggg 
      aggatcact ggactggat cctgccgag ccgtttttt 
      caggggatg cctccccat gtccgtctg gacccttcc 
      gacgccgaa ttcgtcgac gtgatccac accgacggc 
      aaaggcatc atcttcctc ggctacgga atgtcgcag 
      ccttgcggc catttagac ttctatccc aacaatggg 
      aaagagcaa cctggatgt gatattacc caaactcct 
      ttagtacct ctgaccctc atcagggat ggactagag 
      gaagcgtcc agagttctg gtcgcttgt aatcatgta 
      agagccata aaattattt attgatagt ataaacaca 
      cagtgccct tacataggg catcaatgt tccagtttc 
      gagcagttt atggctggg aaatgcttc acttgtaag 
      cctggaact acaaattgc gctgtgatg ggttatcat 
      gcccacact agcccaggg attgaaaat aatgaaatc 
      tcccaatcg aaactgacc gatttggtc gggaataaa 
      tatttcctt accacggga cgagagttc ccgttttgc 
      cgaagactg taccgagtg agaatagaa ttagctaaa 
      ccccgaatg gccgaatcc tgggtccag ggtttcatc 
      aaagcctcc atttacagt tccgctggt gtaatccac 
      gtggattta acaccctct ggtgaaacc aaactggaa 
      cacggcacc agtttcacc agtgtcgtc tcacatcca 
      gccgactta ggtggcaat ataaaaaaa gtcgaactg 
      agttgggag tacgaacta aatgttctc gaaccaaaa 
      actatttgt ctgttttgg tgcaacgat cacttgtat 
      gttgaaagt gtagccgtg gatgagatg gcattgccg 
      ggaagaggg aaaagagat gcgtcgttt tcgaacaag 
      ctttgtccc aaaggaaag aagagttac tcggatata 
      gcaagcagg gggaaagga gtgttcgtt gatttgtgc 
      aaacagtca gataaatac gataagagc tgatattca 
      accagtgat taattaagt tttaataaa ttgttgtta 
      gtaag
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