Status
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Gene information
General information
TC034279
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010964,TC010965
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3393183..3403017
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034279
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02910

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    iBeetle screen iB_04952

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs partially decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 1
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034279 RA
      MWCLFLFIA TFLGVGNLT QNYSREALQ EKYPGFGTE 
      WIPVGQKEN GELIIGYFD DSDDARFIF PPEPKEVVT 
      FTLYTRQHP EGTKIEDSL EKLEGIFDK SKPSKFVTH 
      GWMSSGSAD TCVKIKDGF LKKYDANVF IMDWSEISS 
      NVLYPIPMR ATKSVGDFY SEFLNKLVD SGADPKNFH 
      LVGHSLGAH VTGFGARGV KGKVGRVTG LDPALPGFN 
      MGLVEGGHL DKEDADFVD VIHTCAGYL GMSSSIGHA 
      DFHPNGGSV PQPGCENIF EMIEACSHG RSWAYFAES 
      LTAEVPFMA YRCDSFENF LHKEECKEE GIPMGDPAP 
      TSARGNYYL RTQSEAPFA VKNIQENTC LVGKTALIT 
      GANTGIGYE IALDFAKRG ARVILACRN EQKAREACR 
      KIINETGNE NVVVKFIDL SSFASVRAF VEDVKRSED 
      RLNILVNNA AILEVGLNS LTIDGNLLV MQVNYFSPF 
      LLTVLLTDL MRKSKPSRI INLTSVMAR VAHGFNVDK 
      INNVEGTLN DYGITKLCM ILFTLELAK RLDKSGVTA 
      YSADPGLVD TEIFKQTPR FINLLAQSF KRFFFKTPE 
      EGAQTPIHC SVTKGIEEC NGENFIDCQ RVKKYAIVE 
      NQELTKFWN ITEMIVSLK PDEIYKSED NDKTSISDD 
      YTITSKLCF RQFLFLNKM FYIFVIVFL LIFGVILKI 
      YIKFTTGWC RSKTCLVGK TAIITGANT GIGFETALD 
      FAKRGARVI LACRDEKRA EDARYKVIE ETGNKNVVV 
      KLINMSSFN SVREFAKEI NETEDRLDI LVNNAGAGG 
      IGDKKSKDG HVLLMQINY FSSFLLTHL LIGLLKKTK 
      GSRVINVSS MVAKYAKNF DVSNVDKYP GIVTVYYYS 
      KLCNILFTK ELARRLEGT EVTTYSLHP GAVKTELYR 
      HAKNGYKLL FQFLTNIFF KTSEEGAQT TIYCSVTKR 
      IEKYSGEHF EDCQKVASY KTVADPDLP KKLWEFTQD 
      IVQLKPDEI LT
    CDS sequence
    • >TC034279 RA
      atgtggtgc ctctttcta tttattgct acatttttg 
      ggtgtgggt aatctgact caaaattac agtagggaa 
      gctcttcag gaaaagtat ccgggattt ggtactgaa 
      tggattccc gtaggccaa aaagaaaac ggcgaattg 
      attattggt tactttgac gattcagat gatgctagg 
      tttattttt cctcctgaa ccaaaagaa gtcgtaact 
      tttacactt tacaccaga caacaccct gagggcacc 
      aaaattgag gattccctc gaaaaactt gaaggaatt 
      ttcgataaa agtaaaccg agcaagttt gtgactcat 
      ggctggatg tccagcggt tctgcggac acttgtgtg 
      aaaattaaa gacgggttt ttgaaaaaa tacgacgcg 
      aatgtgttt ataatggac tggagcgaa atctcgagc 
      aatgttctt tatccaatt ccaatgagg gccaccaaa 
      tcggtgggc gatttctat tccgaattt cttaacaaa 
      cttgtcgat tctggtgcc gaccctaaa aactttcat 
      ctggttggt catagttta ggggctcat gtcacgggg 
      tttggggct cgaggtgtg aaaggcaag gttggtaga 
      gtgacaggt cttgaccca gcattgcct gggttcaac 
      atgggtcta gtcgaagga ggacatctt gataaggag 
      gatgctgat tttgtggat gttatccat acttgtgca 
      ggttatttg gggatgagt agttcaatt ggacatgct 
      gatttccac ccaaatggg ggaagtgtg cctcagcct 
      ggatgcgag aacattttt gaaatgatt gaagcttgt 
      agccatggt cgaagttgg gcctatttc gccgaaagt 
      ttgactgct gaagtacct tttatggca tatcgttgt 
      gattcgttt gagaatttc ttgcacaaa gaagagtgt 
      aaggaagaa ggaattcct atgggagat ccagctcca 
      acttcagct cgaggaaat tattattta agaacacag 
      agtgaggcg ccatttgca gtgaaaaat attcaggaa 
      aatacgtgt ttagttgga aaaacagct cttataacc 
      ggagcaaat acaggcatc ggttacgag atagcattg 
      gattttgca aaaagagga gctcgagtg attttagct 
      tgccgaaat gaacaaaag gcaagagag gcatgcagg 
      aaaataatt aatgaaact ggaaatgaa aatgtagtt 
      gtcaaattt atcgatttg agttctttc gcttcagtt 
      agagccttt gttgaagat gtaaaaaga tctgaagac 
      cgtttaaac attctggta aataatgca gcgatcctt 
      gaagttggt ctaaattcc ttaactatc gatggtaat 
      ctgttagtt atgcaagtc aattatttc tcacctttt 
      ctattaact gttctttta acagatcta atgaggaaa 
      agtaaacca agtcgcata attaatcta acatcggta 
      atggcaaga gttgctcac ggttttaat gtcgataaa 
      attaataac gttgaagga actttgaac gattatggt 
      atcacgaaa ctttgtatg atattgttt acactagag 
      ttagccaaa agactagat aaaagtgga gtcactgct 
      tattctgca gatccgggg ttagtggat acagaaatt 
      tttaaacaa acaccaaga ttcataaat ttgctggcc 
      cagtcattc aagcgtttc ttttttaag acaccagag 
      gagggagca caaacccca atccattgc tctgtgaca 
      aaaggaatc gaagaatgt aatggtgaa aactttata 
      gattgccaa agggttaaa aaatatgca attgtagaa 
      aaccaagaa ttgacaaaa ttttggaac attacggaa 
      atgatagtc tcattaaag cctgatgaa atatataaa 
      agtgaagat aatgataaa acaagcata tcagatgat 
      tatacaata acttccaag ttatgtttc cgacaattt 
      ttgtttttg aacaaaatg ttctacata tttgtgata 
      gtgtttctt cttatattt ggtgtaatt ttaaaaatt 
      tacatcaag tttactaca ggatggtgc agaagcaaa 
      acatgctta gtaggcaaa actgctata ataactggt 
      gcaaacaca ggtattggt tttgagacg gcgttagat 
      tttgccaaa aggggggca cgggtgatt ttagcttgt 
      cgcgatgaa aaacgagca gaagatgcc cgttataaa 
      gttatagaa gaaactggc aacaaaaat gttgttgta 
      aaactgatc aatatgagt tcgtttaat tcggtacga 
      gagtttgct aaggaaatt aatgaaaca gaagatcgt 
      ttagatatt ctggtgaat aacgcaggt gctggaggg 
      atcggtgat aaaaagagc aaagacgga catgtttta 
      ttgatgcag attaactat ttttcgagt tttctattg 
      actcattta cttataggt cttttaaag aaaacgaaa 
      ggaagtcgt gtgatcaat gtgtcatct atggtggcc 
      aagtatgcc aaaaatttt gatgtcagc aatgttgat 
      aaatatcca ggaatagtt accgtatac tactactcc 
      aagttatgc aatattctg tttacaaaa gaactggca 
      agaagattg gaaggaact gaagttacg acttattcg 
      ctgcatcca ggcgctgtg aaaactgaa ctttacaga 
      catgcaaaa aatggttat aaactcctt tttcagttt 
      ttgaccaat atatttttc aagacaagt gaggaaggg 
      gctcagaca acaatctac tgttctgtc acaaaacga 
      attgaaaaa tacagtggc gaacatttt gaagactgc 
      caaaaagtt gcatcttat aaaaccgtg gcagatccc 
      gaccttcct aagaaatta tgggaattt acacaagat 
      atagttcag ctaaaacct gatgaaatt cttacatag
    mRNA sequence
    • >TC034279 RA
      tactttaat aaaaatgtg gtgcctctt tctatttat 
      tgctacatt tttgggtgt gggtaatct gactcaaaa 
      ttacagtag ggaagctct tcaggaaaa gtatccggg 
      atttggtac tgaatggat tcccgtagg ccaaaaaga 
      aaacggcga attgattat tggttactt tgacgattc 
      agatgatgc taggtttat ttttcctcc tgaaccaaa 
      agaagtcgt aacttttac actttacac cagacaaca 
      ccctgaggg caccaaaat tgaggattc cctcgaaaa 
      acttgaagg aattttcga taaaagtaa accgagcaa 
      gtttgtgac tcatggctg gatgtccag cggttctgc 
      ggacacttg tgtgaaaat taaagacgg gtttttgaa 
      aaaatacga cgcgaatgt gtttataat ggactggag 
      cgaaatctc gagcaatgt tctttatcc aattccaat 
      gagggccac caaatcggt gggcgattt ctattccga 
      atttcttaa caaacttgt cgattctgg tgccgaccc 
      taaaaactt tcatctggt tggtcatag tttaggggc 
      tcatgtcac ggggtttgg ggctcgagg tgtgaaagg 
      caaggttgg tagagtgac aggtcttga cccagcatt 
      gcctgggtt caacatggg tctagtcga aggaggaca 
      tcttgataa ggaggatgc tgattttgt ggatgttat 
      ccatacttg tgcaggtta tttggggat gagtagttc 
      aattggaca tgctgattt ccacccaaa tgggggaag 
      tgtgcctca gcctggatg cgagaacat ttttgaaat 
      gattgaagc ttgtagcca tggtcgaag ttgggccta 
      tttcgccga aagtttgac tgctgaagt accttttat 
      ggcatatcg ttgtgattc gtttgagaa tttcttgca 
      caaagaaga gtgtaagga agaaggaat tcctatggg 
      agatccagc tccaacttc agctcgagg aaattatta 
      tttaagaac acagagtga ggcgccatt tgcagtgaa 
      aaatattca ggaaaatac gtgtttagt tggaaaaac 
      agctcttat aaccggagc aaatacagg catcggtta 
      cgagatagc attggattt tgcaaaaag aggagctcg 
      agtgatttt agcttgccg aaatgaaca aaaggcaag 
      agaggcatg caggaaaat aattaatga aactggaaa 
      tgaaaatgt agttgtcaa atttatcga tttgagttc 
      tttcgcttc agttagagc ctttgttga agatgtaaa 
      aagatctga agaccgttt aaacattct ggtaaataa 
      tgcagcgat ccttgaagt tggtctaaa ttccttaac 
      tatcgatgg taatctgtt agttatgca agtcaatta 
      tttctcacc ttttctatt aactgttct tttaacaga 
      tctaatgag gaaaagtaa accaagtcg cataattaa 
      tctaacatc ggtaatggc aagagttgc tcacggttt 
      taatgtcga taaaattaa taacgttga aggaacttt 
      gaacgatta tggtatcac gaaactttg tatgatatt 
      gtttacact agagttagc caaaagact agataaaag 
      tggagtcac tgcttattc tgcagatcc ggggttagt 
      ggatacaga aatttttaa acaaacacc aagattcat 
      aaatttgct ggcccagtc attcaagcg tttcttttt 
      taagacacc agaggaggg agcacaaac cccaatcca 
      ttgctctgt gacaaaagg aatcgaaga atgtaatgg 
      tgaaaactt tatagattg ccaaagggt taaaaaata 
      tgcaattgt agaaaacca agaattgac aaaattttg 
      gaacattac ggaaatgat agtctcatt aaagcctga 
      tgaaatata taaaagtga agataatga taaaacaag 
      catatcaga tgattatac aataacttc caagttatg 
      tttccgaca atttttgtt tttgaacaa aatgttcta 
      catatttgt gatagtgtt tcttcttat atttggtgt 
      aattttaaa aatttacat caagtttac tacaggatg 
      gtgcagaag caaaacatg cttagtagg caaaactgc 
      tataataac tggtgcaaa cacaggtat tggttttga 
      gacggcgtt agattttgc caaaagggg ggcacgggt 
      gattttagc ttgtcgcga tgaaaaacg agcagaaga 
      tgcccgtta taaagttat agaagaaac tggcaacaa 
      aaatgttgt tgtaaaact gatcaatat gagttcgtt 
      taattcggt acgagagtt tgctaagga aattaatga 
      aacagaaga tcgtttaga tattctggt gaataacgc 
      aggtgctgg agggatcgg tgataaaaa gagcaaaga 
      cggacatgt tttattgat gcagattaa ctatttttc 
      gagttttct attgactca tttacttat aggtctttt 
      aaagaaaac gaaaggaag tcgtgtgat caatgtgtc 
      atctatggt ggccaagta tgccaaaaa ttttgatgt 
      cagcaatgt tgataaata tccaggaat agttaccgt 
      atactacta ctccaagtt atgcaatat tctgtttac 
      aaaagaact ggcaagaag attggaagg aactgaagt 
      tacgactta ttcgctgca tccaggcgc tgtgaaaac 
      tgaacttta cagacatgc aaaaaatgg ttataaact 
      cctttttca gtttttgac caatatatt tttcaagac 
      aagtgagga aggggctca gacaacaat ctactgttc 
      tgtcacaaa acgaattga aaaatacag tggcgaaca 
      ttttgaaga ctgccaaaa agttgcatc ttataaaac 
      cgtggcaga tcccgacct tcctaagaa attatggga 
      atttacaca agatatagt tcagctaaa acctgatga 
      aattcttac atagggaat aaaacactt cgttctcgc 
      gtttaaacc ttttcgcgg attaatcac attgtaaga 
      cccttatat aatgtgtcc aaaaataaa atggttgtt 
      catca
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