Status
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Gene information
General information
TC031844
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001410,TC001411
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451510.1: 20716..60613
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031844
    iBeetle screen iB_02908

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    iBeetle screen iB_06734

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031844 RA
      MPAPELNGP SSTNGSPLS TDRFTVFQS SQPISVRAT 
      YGPFSTKQT VPARYVVPD PLPLNTTGP AIDLQDLAT 
      HHLDMSAHI VRNEIPRDS PVLRVLFHT GTDPGGRRQ 
      ILLTRHHQR VCIVLHATL GTKPPLHAA CSPDGEDGV 
      CLAQITIPS SWWPPLPSP DKDGRPGKI TKTPPRLVQ 
      VAYSVMEPR PEDAEGCHP KMQIQPSTV LGSVPLVPP 
      KGAYKELKL TDAITMLLP HPPLFPKSR MHVPVFIDK 
      EKAKMLTAI VISSVYVDG SEARGSVNG SVLVKYGTY 
      IGLARFTVW MPEFPLDLQ VTDTRLSQL KSWRVPDYH 
      PSGVKSRRK KRSYASNWG GSNPPDDLG NVVEKPICR 
      LRYQQSPVD VYAHFMAVD HDSGRISYL INRRTWLRV 
      TDLVLPLLR VSDPRIASL HGRILQGRS MGRTEVQVL 
      SPITGRVIG AKEIRVGND KVGLSRLLV QVVSGLQLN 
      ISPDSSIEN GYIAETSVT RKLTAQYQE GLLDIELEF 
      SDGTRTPLR DIADSDYHL VVESLDPEV VAFAPMVAS 
      HHPRVIAVG EGNGDLLQV TLLLAEECR TSNRPKMKG 
      AGPLATASA SVTVDFTSS DLQHRPDIL QNDGGSYGG 
      SKREFQDLQ DILKGTPLK DDDSQEPNV QARQYQGGK 
      GVLRQHSPQ HMTPLEISM YILLGTFCC AIVVFVVSC 
      VVYASKFKA EEGAIAPGE RAAPVAPGS LVVHRKPRE 
      TTTNAHDWV WLGRATLDS SNRNSSVAN TNGNEMRIT 
      ANPLNLNYC DPDDCLATS FSNPSHIEL PSSSQADIN 
      SNRPIDSNT YCKSKSGGS NNEDLQVWN KPTPPPPLP 
      PHGVPLQPK PPQPVEDYR PPVPPHRNI GVTSEATPP 
      RKHHHHHHH RNSKHQDKH HHDYGWAVN GWSKDQEEE 
      DEDLVMTAS QEPMRSVFE FDDEPPKEE EKPEEVKLR 
      SKPPKPDEE EVQFVQYPK SPNANGNGE LVPV
    CDS sequence
    • >TC031844 RA
      atgccggca ccggaacta aacggtcct tcctccacc 
      aacggctct cctctctcg accgatcgc ttcaccgtg 
      ttccaaagc tcgcagcct atctcagtc cgcgccacg 
      tacggccct ttcagcacg aaacagacc gtaccggcg 
      cgctacgtc gttccggac cctctgccg ctcaacaca 
      acaggccct gccatcgac ttgcaagac ctcgccacg 
      caccacttg gacatgtcg gcccacatc gtccggaat 
      gagatcccg cgggactcc cccgtgctc cgggtgctc 
      ttccacacc ggaacagat ccgggcggc cgcagacaa 
      attctgtta acacgccac caccagcgc gtgtgtatc 
      gtgctccac gccactctg ggcactaag ccgcccctc 
      cacgctgcc tgcagcccc gacggagag gacggcgtg 
      tgtttggcg caaattacg ataccgtct tcgtggtgg 
      cccccgttg ccgagtccg gataaggac ggaaggccg 
      ggcaaaatc accaaaacg ccgccaagg cttgtccaa 
      gtggcgtat tcagtgatg gaaccgagg cctgaagat 
      gcggaaggt tgccatccc aaaatgcaa atacagccg 
      tcgacggtg ttaggcagt gtgccgctt gttccgcca 
      aaaggtgcc tacaaggag ctcaaactg acagatgct 
      ataacgatg ttactgccg catccgccg ttgtttccc 
      aagtccagg atgcacgta ccggttttt atcgacaag 
      gagaaggcg aaaatgcta acagcgatt gtcataagc 
      tctgtttac gtggacgga agtgaggcc cgcggttcc 
      gttaacggt tcagttctc gtaaaatac ggcacttat 
      attggcctc gcccgcttc actgtctgg atgcccgaa 
      ttccccttg gacctccaa gtgaccgac acgaggctg 
      tctcagctc aagtcgtgg cgcgtcccg gattaccat 
      cccagcggt gttaaatcc cgccggaag aagcgatcc 
      tatgccagt aattgggga ggctctaat ccccccgat 
      gacttgggg aatgtggta gaaaagccg atttgccgg 
      ctgaggtat cagcagagc ccagtggac gtttatgcc 
      cacttcatg gccgtggac cacgactcc ggaaggatc 
      agttatctg atcaatcgg aggacgtgg ttaagggtc 
      acggacttg gtcttgccc ttgttaagg gtgagcgac 
      ccccgcatt gcaagcctg cacgggagg atactccaa 
      gggcgcagc atgggccgg accgaggtg caggttctg 
      agccccatc acgggacgt gtcattgga gccaaggaa 
      atcagagtc gggaatgac aaagtggga ttatcgcgg 
      cttctggtc caagttgtc tcaggatta caactaaat 
      atctcgccc gatagttcg atcgaaaac ggctacata 
      gccgagaca agtgttaca aggaaatta acagctcaa 
      taccaggag ggcctgctt gacatcgag ctggaattt 
      tccgatggg actagaacc ccactccgg gatattgca 
      gactcggat tatcacctc gtggtcgag agtctggac 
      ccagaggtg gtggcgttt gcccccatg gtggcgtcc 
      caccaccca cgcgtgata gcagtgggt gaaggcaac 
      ggagacttg ctacaagtt actctattg ttagctgaa 
      gaatgtaga actagcaat aggccaaaa atgaagggc 
      gctgggcct ctggccact gcctcagct tcggtcact 
      gttgacttt acttcaagt gacttacaa cacaggccc 
      gatatttta cagaatgac ggggggtcg tatgggggc 
      tccaagagg gaatttcag gatcttcag gacatttta 
      aaaggcact cccttgaaa gacgacgac tcgcaggag 
      cccaacgtg caggcccgc caataccaa gggggcaaa 
      ggggtgctg cggcagcac tccccccag cacatgacg 
      ccgctcgaa atcagcatg tacatcctc ctgggcacg 
      ttctgctgc gccatcgtt gttttcgtg gtatcgtgc 
      gtggtctac gcgtccaag ttcaaggcg gaagaaggc 
      gcgattgct ccgggtgag agggccgct ccggtggcc 
      cccggaagc ctggtagtg catcgcaaa ccccgagaa 
      accacgaca aacgcccac gattgggtc tggctaggt 
      cgtgccact ttggacagt tcgaatcga aattcctca 
      gttgcgaac acaaacgga aacgaaatg agaattaca 
      gcgaatccg ctcaatttg aattattgc gatccggat 
      gattgtcta gcgacgagt tttagtaat cctagtcat 
      atcgagctg ccgagctcg tcgcaggcc gatattaat 
      tccaatagg ccgatcgat agcaacacc tattgcaag 
      agcaagagt ggaggcagc aacaatgag gatctacaa 
      gtgtggaac aaaccaacg ccgcctcca ccgctcccc 
      ccgcacggc gtgcccctc cagccgaag cccccgcag 
      ccggtggag gactaccgg ccccctgtt ccgccccat 
      cgcaacatt ggagtgact tccgaagcc acccctccc 
      cgcaagcat caccaccac caccatcac aggaacagc 
      aaacaccaa gacaagcac caccacgac tacggatgg 
      gccgtcaac ggctggtcc aaggaccag gaggaggaa 
      gacgaagac ttagtaatg acagcgagc caggaaccc 
      atgcgcagc gtgttcgag ttcgacgac gagcctccg 
      aaggaggaa gagaagccg gaagaggtg aagctgcgg 
      agcaagccc ccgaagccc gacgaggag gaggtccag 
      ttcgtgcag tacccgaag tcgccgaac gcgaacgga 
      aacggtgag ctggtccct gtttga
    mRNA sequence
    • >TC031844 RA
      ttccagctt actcagaat gcgtgaacg tacatttcg 
      aaaacaaag acggaggct tcttcctga aacaatctc 
      ctcgtcagc atttcatgc cggcaccgg aactaaacg 
      gtccttcct ccaccaacg gctctcctc tctcgaccg 
      atcgcttca ccgtgttcc aaagctcgc agcctatct 
      cagtccgcg ccacgtacg gccctttca gcacgaaac 
      agaccgtac cggcgcgct acgtcgttc cggaccctc 
      tgccgctca acacaacag gccctgcca tcgacttgc 
      aagacctcg ccacgcacc acttggaca tgtcggccc 
      acatcgtcc ggaatgaga tcccgcggg actcccccg 
      tgctccggg tgctcttcc acaccggaa cagatccgg 
      gcggccgca gacaaattc tgttaacac gccaccacc 
      agcgcgtgt gtatcgtgc tccacgcca ctctgggca 
      ctaagccgc ccctccacg ctgcctgca gccccgacg 
      gagaggacg gcgtgtgtt tggcgcaaa ttacgatac 
      cgtcttcgt ggtggcccc cgttgccga gtccggata 
      aggacggaa ggccgggca aaatcacca aaacgccgc 
      caaggcttg tccaagtgg cgtattcag tgatggaac 
      cgaggcctg aagatgcgg aaggttgcc atcccaaaa 
      tgcaaatac agccgtcga cggtgttag gcagtgtgc 
      cgcttgttc cgccaaaag gtgcctaca aggagctca 
      aactgacag atgctataa cgatgttac tgccgcatc 
      cgccgttgt ttcccaagt ccaggatgc acgtaccgg 
      tttttatcg acaaggaga aggcgaaaa tgctaacag 
      cgattgtca taagctctg tttacgtgg acggaagtg 
      aggcccgcg gttccgtta acggttcag ttctcgtaa 
      aatacggca cttatattg gcctcgccc gcttcactg 
      tctggatgc ccgaattcc ccttggacc tccaagtga 
      ccgacacga ggctgtctc agctcaagt cgtggcgcg 
      tcccggatt accatccca gcggtgtta aatcccgcc 
      ggaagaagc gatcctatg ccagtaatt ggggaggct 
      ctaatcccc ccgatgact tggggaatg tggtagaaa 
      agccgattt gccggctga ggtatcagc agagcccag 
      tggacgttt atgcccact tcatggccg tggaccacg 
      actccggaa ggatcagtt atctgatca atcggagga 
      cgtggttaa gggtcacgg acttggtct tgcccttgt 
      taagggtga gcgaccccc gcattgcaa gcctgcacg 
      ggaggatac tccaagggc gcagcatgg gccggaccg 
      aggtgcagg ttctgagcc ccatcacgg gacgtgtca 
      ttggagcca aggaaatca gagtcggga atgacaaag 
      tgggattat cgcggcttc tggtccaag ttgtctcag 
      gattacaac taaatatct cgcccgata gttcgatcg 
      aaaacggct acatagccg agacaagtg ttacaagga 
      aattaacag ctcaatacc aggagggcc tgcttgaca 
      tcgagctgg aattttccg atgggacta gaaccccac 
      tccgggata ttgcagact cggattatc acctcgtgg 
      tcgagagtc tggacccag aggtggtgg cgtttgccc 
      ccatggtgg cgtcccacc acccacgcg tgatagcag 
      tgggtgaag gcaacggag acttgctac aagttactc 
      tattgttag ctgaagaat gtagaacta gcaataggc 
      caaaaatga agggcgctg ggcctctgg ccactgcct 
      cagcttcgg tcactgttg actttactt caagtgact 
      tacaacaca ggcccgata ttttacaga atgacgggg 
      ggtcgtatg ggggctcca agagggaat ttcaggatc 
      ttcaggaca ttttaaaag gcactccct tgaaagacg 
      acgactcgc aggagccca acgtgcagg cccgccaat 
      accaagggg gcaaagggg tgctgcggc agcactccc 
      cccagcaca tgacgccgc tcgaaatca gcatgtaca 
      tcctcctgg gcacgttct gctgcgcca tcgttgttt 
      tcgtggtat cgtgcgtgg tctacgcgt ccaagttca 
      aggcggaag aaggcgcga ttgctccgg gtgagaggg 
      ccgctccgg tggcccccg gaagcctgg tagtgcatc 
      gcaaacccc gagaaacca cgacaaacg cccacgatt 
      gggtctggc taggtcgtg ccactttgg acagttcga 
      atcgaaatt cctcagttg cgaacacaa acggaaacg 
      aaatgagaa ttacagcga atccgctca atttgaatt 
      attgcgatc cggatgatt gtctagcga cgagtttta 
      gtaatccta gtcatatcg agctgccga gctcgtcgc 
      aggccgata ttaattcca ataggccga tcgatagca 
      acacctatt gcaagagca agagtggag gcagcaaca 
      atgaggatc tacaagtgt ggaacaaac caacgccgc 
      ctccaccgc tccccccgc acggcgtgc ccctccagc 
      cgaagcccc cgcagccgg tggaggact accggcccc 
      ctgttccgc cccatcgca acattggag tgacttccg 
      aagccaccc ctccccgca agcatcacc accaccacc 
      atcacagga acagcaaac accaagaca agcaccacc 
      acgactacg gatgggccg tcaacggct ggtccaagg 
      accaggagg aggaagacg aagacttag taatgacag 
      cgagccagg aacccatgc gcagcgtgt tcgagttcg 
      acgacgagc ctccgaagg aggaagaga agccggaag 
      aggtgaagc tgcggagca agcccccga agcccgacg 
      aggaggagg tccagttcg tgcagtacc cgaagtcgc 
      cgaacgcga acggaaacg gtgagctgg tccctgttt 
      gaaggcaaa ccaactgag cgcggtttc aggcgcagc 
      agcgcggag gtgaagcgc gccaccatt gtggggaac 
      cccatgttc agcagcgcg gacgcgccc cttccggcg 
      acctgccgg gnnnnnnnn nnnnnn
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY