| General information | |||
| TC006465 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 10075361..10099948 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006465 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02906 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 22.22% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
Days post injection
- >TC006465 RA
MEWSTGDLI WFDPGVGHW LPGEVLECH RSANVLTVQ AVINGKPQT FALTEGEGS VRRRQDLGP GGVEDMIQL TDLHEAALL WNLKLRYDR NLIYTYAGS ILVAVNPYR MFDSSYGIE AAQRYRGKM IGALPPHLF ALGASAYSA LPAPQVVVI SGESGSGKT ESTKLVMQY LAAVAPSAP RGQALVTEQ ILEAAPLLE AFGNARTAR NDNSSRFGK YLEVYFKYG AIVGAKVTQ YLLEKSRII TQAPGERNY HVFYELLGG LSNTERQKY GLVDADKYF YLNQGGGDC APGHSGSGA DWGALTRAM QVLGVGEAE QEGIIKVLA SVLHLGNVY FHRRQLRHG QEGVEVGSD VEIKWAAHL LQISPSGLQ RALTSRITE ARAERVYSP LSIDQALDA RDAFAKALY SALFNWLVT RVNSIVQRG GLHDAARIS LLDIFGFEN LNENSFEQL CINYASESL QLYFNKHVF KLEQQEYAR ERLEWTNMT WMDNTPVIH LLGKKPVGI LHLLDDESN FPRASDASF LEKCHYNHA LNEHYCRPR VGGREFGIR HFAGQVWYS VDGFLDKNR DALRPEVVE LISSSKEPL VASIAKPLI NQTQSRTLP KGTDGRFVT MKPRTPTVA ARFSDSLQQ LLESMARCN PWFVRCIKP NNDKSPMRF DMPVVLEQL RYAGMLDTI KIRQSGYPV RMKFQQFVE RYRYLLSGV LPRGAPYRD LCRAILEQM PSTGAEGPD YQLGATRVF LRENLQRQL EVKRSDCLR DSAIVIQKH IRGYLARKN YKNLRRSTV TIQKHWRGY KHRKQFKTI RHGVIKAQA LVRGRRERK RFAQRKADF KRRVEAEKL AKERAKQRA AREAQLQKA APKSSVHHL EIPAELAFI FSKIENFNP PHTERNLIK VLGGVTGTI PPLNLPHDL NQYEFSRFS SVYFKGADL GMRKEPITA PFLSKAATR DQDFQDAVA LFKLILRWS NDGQLSGPR ERALADFIV YKALNSRGL RDELLVQLC NQVWRNENS DRIWQLMAH CLSCFQPSP PLSKYLLKF ITDHASANY KELLQRKLT RSLQKAPHA RSLPPTLLE WRSTRQRTN TALPLTLPD NNVASVNVD SWTTCEEAA SIAINSLGL TAEGWSVVM DDAGIVSEG SGLDYIMDL VSELELCPA FPISKSSLL KVGTRRASP AEIDHSPTT PTRPQVPPP EPPTHLNRK ISREMARQS PPHHKSNQV ASRKVSRES HREYSPPVT STPARKSSH EALSRNSAL NERYFDIEK VRSRSLDNL LSEPEPTPL SDLGLSQSR LNDRYHSVE QLAPIKPVL ANQNYMSKQ EIDFEYPDV SSVSTSHRG GPRYIKSQY TGKKAPPGS HSSRAHIEK SEFGVRSSA MSDTSETPS LASHVRRVR VPSQASDVD QFLDELFSP VLDGNLDEL SDARSLAAS IKGGTRDDY SDFLDDVLG DSLDEYLDM SNAGFIARK IKGGGHRNR RDSQGSSVL DQEVEKLTS GTSQPNTAR TSADKGNVD DYISDLFRP IFINDSLKN LTEKHNLVD SIKGGGTTH QQNGAGPSY TFPTIPAPI VGSPPVLMP LLSPTQEGF MPVYNVPPP NGTDMATYQ QSLQRAFLQ SAMAQNIQI QQQLLAQNQ ALQQLLTQQ IPTEVKVNE AIQTTVKAQ VHQSSPNRK SSFKTSNST RKSSSPSTN DYKSRKTSS DSNMSMRNG VPPPPPMPP PLDGTDPSE ARPFLDPYG RAKTVRIGK WRWPPPKDE KVAENGEDF MQFKMRQNQ RKVTPVRDQ PIVTNGHPK VEQSSAEWE EIEFEPIVR EPERPTKVS SKRSFEIGA SRPSPGSVG KLKLSSEMR QRLEKVTAN HSVRSTSSH VEKPARAVN KLEDTRKMM LEQQLAGRW GDDSQDLPS GKSSPVTPP HISHNGSTS PTQQSWSSS NWKPGPPPP PIGPNSLPP APPGPAPPP PVVRPNQPP LPIEPVRES FMAQRQDRD TFGVHQNRV LQNNSKRNS FSANWEVQS AITHETQDD STHWAKDAL DLDKTDGRL SRDSWDQAD STTITSSDQ PRRIISERW ETERKRVGK ETGERPTFR THQFNKSAQ EREKKHSIA STQMTDKTD RNDVPEWPE FMRPIHTPP PKSPVIHRS QDQVQTQSP KTTHRLLPL GASACVTYN RVSWLLRVR KEVFSPNEA LGPPSILHL IFCQIVGDV YGLTPCLRL TQTEKRAGV NMLSGYGIT AENYNSSHR ANIKRNVIE LARTWPLYF ARLFPVSGA AQLSDVQLV AVSHWGIHL VKREQTHLQ VLKSFPLKD IASCTAPRP TTVSFDGPQ GRLSLHTPR AQQLSEMVT KYCAENRKS SSSPRSPVA GKSFETQPR YRRHCDVAL LSGKMNKTK WAEISKYKP SDDFRANSL DSLANSSNP SESLSDDSH DSDSGTDTK SKNSFTVKC EVYAQNPCG SIRSWTKSE SSNSDGYDF VASDSLGEK KQDVISSRM ETIPEEAEP KVSVKEILA RFENLKDKD GKDCNNNQV KSNGITNNI KNIANGATK NIKNTVVNK SEASIVSAK PKSDNSVVE KNCVNSANK TNASKTNVN KSKKTENTE TSATAPAKP QTKELQMKL SPPQRVPPV EQRSEVSPR KANNVPETR LAASPTEMR GEMRGERAQ SPSSPLPPT GKYSLMQFA MHNFRQTAD LELLKMDSS IKSKEKRKE WTWKQQTDL IKWQGTPID GPLLRLEDP ELGPLAVEC FECILRYCG DLPLTPEMP EVKCVYTVL MHCHKHAQL RDEVYCQLM KQTTSNKSE SPESCQRAW RLLSIVAAY FACSDNLLP FLTEHLSSA ASDRRRVCH GTAAVCLAN LRKTQRCGG RKNVPSVEE VTAVSAGRS ARRQIYRLP GGAERVVNT RCSTVVADV IAELCSLIG VGTEAEQQE FSLYCIVQG DAFTMPLAA DEYILDVTT ELHKAAQPF YLIFCRSVW YHPLKHDAS PLYTEVLFN QVAPDYLEG LLLRLGNPN LPPSVIRDM ALIAALLHR AADLNHPPS LKEVKFLLP KPVLGLREP RPPQWLALV QTSWGQAAG LQVARAKHR VLQILSNWP LFGSSFFAV KRVSGDLEN WQEHILALN RSGVHFLDM TSHETLQHW PFAEVISTR KVRSEDGAL FLDMKCGNL LQQRVTRLQ TEQAHEISR LVRQYINLE QASSARN
- >TC006465 RA
atggagtgg agtaccggc gacctgatc tggtttgac ccaggagtg ggccactgg ctccctggc gaggtccta gaatgtcac aggagtgcc aacgtgtta accgtccaa gcagtcatc aacgggaag ccgcagacg ttcgctctg acggagggc gagggctcg gtccggcgc cgccaggac ctgggcccc ggcggcgtc gaggacatg atccaactc accgacttg cacgaagcc gccttatta tggaatctc aagctgcgg tacgaccgg aacttgatc tacacgtac gcgggaagt attctcgtg gccgttaac ccctacagg atgttcgac tccagctac ggcatcgaa gcggcgcag agatacagg ggcaagatg atcggcgct ttgcctccg catctcttc gccttgggc gcttccgcc tactcggcg ctgcccgcc ccccaggtg gtggtgatc agcggggag agcggctcc ggaaagact gaatccacc aaactcgtc atgcagtac ttggcggcg gtggcgccg tccgccccc agaggacag gccttggtc acggagcag atcctcgag gcggctccc ttgcttgaa gctttcggg aatgcgcga acggcacgc aatgataac agttcgcgg tttgggaag tacctcgag gtttatttc aagtatggg gcgatcgtc ggggctaaa gtgactcag taccttttg gagaagtcg aggattatc actcaagcg ccgggcgaa cggaattat cacgttttt tacgaactt ttgggcggt ttgagtaac acagaaaga cagaagtac ggtttagtc gacgctgat aagtacttc tatcttaac caagggggc ggcgattgt gcgccgggt cattccggc tcgggggcc gactggggg gctctgaca agagccatg caagtgtta ggtgtcgga gaggcggaa caagaagga ataattaag gttctagcc tcggttctg cacttggga aatgtctat ttccataga aggcagctc cggcatggc caggagggt gtggaagtt gggtccgat gttgaaatt aagtgggct gctcatctt ttgcaaatt tcccccagt ggtttacaa agagccttg acttcaaga atcacagaa gctagagcc gaacgcgtc tactcacct ttaagtata gatcaagcg ctagacgcg agagatgct ttcgccaaa gctctgtat tctgccctt ttcaattgg ttagtgacg cgtgtcaat tcaattgtc cagcgagga ggactacat gatgcggcc cgaatttcg ctacttgac attttcggg tttgagaat ctaaacgag aattccttc gaacaatta tgcatcaac tatgcctca gaatcgttg caattgtat ttcaacaaa cacgtcttt aaactggaa caacaggag tatgcaagg gagcgtcta gaatggacc aacatgact tggatggac aacacgcca gtcatacat ctcttgggc aaaaaacca gtaggtatt ctccacctt ttagacgac gaaagcaac ttccccaga gcttccgat gcttcgttt ttggaaaag tgtcactat aatcacgcc ctgaacgag cattattgc cgtccgaga gtcggaggc cgggaattt gggattaga cacttcgcg ggacaagtg tggtattcc gttgacggt ttcctggac aagaaccgg gacgctttg cgtccggaa gtggtcgag ttgatttca tcaagcaag gaaccgttg gttgcgtcc attgccaag cctttaatc aaccaaacg caaagtcgt acccttccg aagggcacc gatggccgt tttgtcaca atgaaaccc agaaccccg acagttgcg gcccgcttc agtgacagt cttcagcaa ctgttagag tccatggct cggtgcaac ccttggttc gtccggtgc atcaaaccc aacaacgac aagagcccc atgcggttt gacatgccg gtggttctc gagcaacta agatatgca ggaatgttg gacactatt aaaatccgc cagtcggga tatccggtc aggatgaag tttcagcaa ttcgttgag agatatagg tatttactt tctggcgtg ttgccaaga ggtgcgccg tatagagac ttatgtcgg gccattctg gaacaaatg ccttccacg ggagctgaa ggtccagat taccaatta ggagcaacc cgcgtcttc ctccgagaa aacctccaa cgccagttg gaagtgaaa cgctccgac tgtctccga gactcggcc atcgtgatc caaaaacac atcagaggg tacttggcg cgcaaaaac tacaagaac ctccgccgc agtaccgta acaatccag aaacattgg cgtggttac aaacaccgc aaacaattc aaaacaatc cgacatgga gtcatcaaa gctcaagct cttgtccgc ggacgcaga gaacgcaag cgcttcgcg caaagaaaa gccgacttc aagcgacgc gtcgaggct gaaaaactg gctaaagaa cgtgctaaa caaagagcc gcccgagag gcacaacta cagaaagct gcacccaaa tcaagcgtc caccatctg gaaattcca gcagaactg gctttcatt ttctcaaaa atcgaaaac ttcaacccg ccgcatact gaaagaaac ctaatcaag gttctgggc ggtgtcact ggaacaatc ccgccgcta aacctaccg catgacttg aaccaatat gagttctcc cgattttcc tcggtttac ttcaagggg gccgacctc gggatgcgg aaagaacca atcactgca cccttcctg tccaaggct gcgactaga gaccaggat tttcaagac gcagtggcg ctgtttaaa ctgattttg cgctggtct aacgacggg cagttgagc ggaccgcgc gagcgggcg cttgccgat tttatcgtg tacaaggcg ctgaattcg agaggattg agagacgag ttgttggtg cagttgtgt aaccaagtg tggcggaat gagaattcg gaccggatt tggcaactg atggcgcat tgtctcagt tgtttccag ccgagcccg ccattgagc aagtatcta ctaaaattc ataaccgac cacgcgtcc gccaattac aaagaattg ctgcaaagg aaattgacg cgaagtttg cagaaagca ccacatgct cgctccctt cctccgact ctcctcgaa tggcgcagt acccgccag cggacgaac acagcccta cctttaact ctccctgat aataacgtg gcttcggtc aatgttgac tcttggacg acttgcgag gaagccgca tctatcgcc ataaactcg ctgggctta accgccgaa ggctggagc gttgtgatg gacgatgcc ggtatagtc tcagaagga agtggcttg gactacatc atggacctc gtatccgaa ctagaatta tgccccgct tttcccata tctaaatcc tctctcctt aaagtcggt actcgcaga gcatcaccg gccgaaatc gatcactca cccacgacc ccaacccgg ccccaagta ccaccaccg gaaccccca actcacctc aatcggaaa atatcacgg gaaatggcc cgccaatca ccccctcac cacaagtcg aaccaagtg gcctcccgg aaagtttcc cgtgagagt caccgtgag tactcacct ccggttact agtacccca gctaggaaa tcctcacat gaggcgctt tctcgcaat tctgccctg aacgaacga tactttgat atagagaaa gtgaggtct cggagttta gataatttg ttgagtgag cctgaaccg accccgctc tccgatttg ggtttgagt cagtcgaga ctgaacgac cggtaccac tctgttgag cagttggcg ccgattaag ccagttttg gccaatcag aattacatg tcgaagcaa gagattgat tttgagtac ccggacgtg tccagtgtt agtacctca caccgggga ggtccgagg tacatcaag tcacaatac accggcaag aaggcacct ccagggtca cactcgagc cgtgcacat atcgagaaa tcagaattt ggagttagg agttcggca atgtctgat actagcgaa actccgagt ttggcaagt catgttaga agagtgagg gttccaagc caggcgtcc gacgttgac cagtttctg gacgagttg ttcagtcca gtccttgac gggaatttg gacgagtta agcgatgct cgttccctg gctgctagt atcaaaggt gggacacgg gacgactat tccgatttt ctcgacgat gttttgggg gattctctt gacgaatat ttggatatg tctaatgct gggttcatt gcaaggaaa ataaagggt ggggggcac cggaaccga cgtgacagt caaggtagt tccgttctg gatcaggaa gttgagaaa ttgacttcc ggtacttca caaccaaac acagccagg acttcggcc gataaaggc aatgttgat gattacatt agcgatttg tttagaccg atttttata aacgatagt ttgaaaaac ctgactgag aaacacaat ttggtcgat agtatcaaa ggtgggggt actacccat caacaaaac ggtgctggt ccgagttac acattcccg accattccc gcacccata gtgggctca ccaccggta cttatgccc ttgttaagt cccacccag gagggtttc atgcctgtt tacaacgtc ccacctccg aacggtacc gacatggcc acataccaa caaagcctc cagcgcgcc ttcctccag tcagcaatg gcccagaac atccaaatc caacaacaa ctcctagcg caaaaccaa gccttgcaa caactacta acccaacaa atcccaacc gaagtcaaa gtcaacgaa gccatacaa accaccgtt aaagcccaa gtccatcaa tcatcaccc aatcgtaaa tcaagcttc aagacgtct aacagtacc cggaagagc agttctcca agcacaaac gattacaaa tcacgcaaa accagctcc gactctaac atgagcatg cggaacggt gttccaccc ccgcccccg atgccgccg ccactcgac ggaaccgac cccagcgaa gcccgcccg tttctggac ccgtatgga cgcgccaag accgtccga atcggcaaa tggcggtgg ccgcccccg aaagacgaa aaagtggcc gaaaacggg gaggatttc atgcagttt aagatgcgc cagaaccag aggaaagtg acaccggtt agggaccaa ccgattgtg acaaacgga cacccaaaa gtggagcag tcgtcggca gaatgggaa gaaatcgag ttcgaaccc atcgtgcga gagccggaa cggccgact aaagtgagt tcgaagcga tctttcgag attggcgcc tccaggcca tcgccaggc agcgtcggc aaactcaaa ctcagttct gaaatgcgc caaaggctc gaaaaagtc actgcgaac cactcggtg cgctcgacg agtagtcac gtggaaaaa ccagcccgg gctgttaac aaactggaa gatacgcgg aaaatgatg ctggagcaa cagctagcc gggaggtgg ggcgacgac tctcaggac ttacccagc gggaagtca agtccagtc actccgcct catattagt cacaacggg agcacctcc ccgacccag caaagctgg tcgagttcc aactggaaa cctggccct cccccccct ccaatcggg cccaactcg cttccccca gcccctccc gggccggct ccgccccct cccgtggtc cggcccaat caaccccca ctcccaatc gagccagtg cgtgaatcc ttcatggcc cagcgccaa gaccgggac acttttggc gtccaccaa aaccgagtt ttacagaat aattccaaa aggaactcg tttagtgcc aattgggag gtacagagt gccataacg catgaaact caagacgat agcacccat tgggctaag gatgcacta gatttagac aaaacagat gggcggttg tcgcgagac agctgggac caagctgac tcaacaacg attacttcg agcgatcaa ccgcgaagg attataagt gaacgttgg gaaaccgaa aggaagaga gttgggaag gaaacaggg gagagaccg acgttcaga acacaccag tttaataaa agcgcacaa gagagggag aagaagcat tcgattgcg tcgacgcaa atgacggac aagacggac cgaaatgac gtgccagag tggcccgaa ttcatgcgg cccatccac acgccgccc ccgaaatcg cctgtgatc caccggagc caggaccag gtccaaacc caatccccc aagacaacg caccgactc ctgcctttg ggcgcctcg gcttgtgtg acctacaac cgggtatcg tggcttttg cgcgtgaga aaagaagtt ttctccccg aacgaagct ctcggcccg ccttcaatc ctccacttg atcttttgc caaatcgtt ggcgacgtc tacggcctc accccttgt ctccgactc acccaaacg gaaaaacgc gctggcgtt aacatgtta tcagggtac ggcataacc gccgaaaac tacaatagt tcccatcgg gccaacatc aagcgaaac gtgatcgag ttggcccgc acgtggcct ttgtacttc gcccgcttg ttcccagtt tcaggggcg gcacagtta tcggacgtc caactcgtg gctgtgtcc cactggggg atccacttg gtcaaaagg gagcagacc cacctccag gtgctcaaa tcgttccct ttgaaagac atcgcctcc tgcaccgcc ccccgaccc accaccgtc agtttcgac ggcccccag ggccggctc agcctccac acccctcgg gcccaacaa ctgtccgaa atggtgaca aagtactgc gctgagaat cgcaagagc tcatctagc ccgcgctcg ccagttgcc ggcaagtca ttcgaaacg cagccacgg taccgtcga cactgcgat gttgcgctt ctttccgga aaaatgaac aagacaaag tgggccgaa atctcgaaa tacaagccc tccgacgac ttccgggcc aactccttg gacagcttg gccaactcc tccaacccg tcggagagc ctcagcgac gactcgcac gactcggac tcgggcacc gacaccaag tccaagaac tccttcacg gtcaagtgc gaggtctac gcgcagaac ccctgcggg agcatccgc tcctggacc aagtcggag agcagcaac tcggacggg tacgatttc gtcgccagt gactcgcta ggggagaag aaacaagat gttatctcg tcgcggatg gagacgatc ccagaggaa gcggaaccg aaagtctca gtcaaggag attctagcg cggtttgag aatttgaag gataaggat ggcaaggat tgcaacaac aatcaagtg aagagtaat ggaattacc aacaatatt aaaaatatc gccaatggg gccaccaag aatattaag aatacggtg gttaataaa agtgaagcg agtattgtc agcgctaaa ccgaagtct gataacagc gtcgttgaa aaaaattgt gtgaacagt gctaacaaa actaacgct agtaaaaca aatgttaat aaaagtaaa aagactgaa aatactgag accagtgcc accgcgcct gcgaaacct cagaccaag gagttacaa atgaaatta tcgccgccg cagagagta ccaccagtc gaacagcgc agcgaagtt tcgccaagg aaggccaac aatgtgccg gaaacgaga ctcgccgcc tctccgacc gagatgaga ggcgaaatg aggggcgaa agggcgcag tcccccagc agccctctg cccccgacc gggaagtac tccctgatg cagttcgcc atgcacaac ttcaggcag acggccgat ttggagctc ctcaagatg gactcttcg atcaaatcc aaagagaag cgcaaagaa tggacctgg aagcagcaa accgacctg atcaagtgg caagggacg cccatcgac ggccctctg ctgcgcctg gaggacccc gagctgggc cccctggcc gttgagtgc ttcgagtgc attctgcgg tactgcggg gacctgccc ctaaccccg gaaatgccc gaagtcaag tgtgtctac acagtgctg atgcactgc cacaaacac gcccaatta cgtgacgaa gtctactgt cagctcatg aagcagaca actagtaat aaatccgag tcaccagaa tcctgccaa cgcgcttgg agactcctt tcaatcgtt gcagcgtac tttgcttgc tcggacaat ctcctaccg tttttgacg gaacacctc tccagcgcc gcttcggac agacgtcgc gtctgtcat ggcactgcg gccgtctgc cttgcaaac ctccgaaag acccagcgg tgtggaggc cgcaagaac gtcccttcc gtcgaggaa gtgacagcg gttagcgcc ggacgcagt gcccgccgc caaatctac cgcctccct gggggagcc gaacgcgta gtcaacaca agatgtagc acggttgtg gccgacgta atcgccgaa ctgtgcagt ctgatcggc gtcggcacc gaagccgag caacaagag ttcagtttg tactgcata gtgcaaggg gacgccttc accatgcct ctcgccgcc gatgagtac atcctggac gtcaccacc gagctgcac aaagccgcg cagcccttc tacctgatt ttctgccgc tcggtctgg taccacccc ctcaaacac gatgctagt cccctctac accgaagtc ctgttcaac caagtggcc cccgactac ctcgaaggc ctcctcctg cgcctaggc aaccccaac ctccccccc agcgtcatc cgcgacatg gccctcatt gccgcgctc ctgcaccgc gccgccgac ctgaaccac cccccgagc ctcaaggaa gtcaagttt ctgctcccg aagcccgtc ctgggcctg cgggagccc cggccgccg cagtggctg gctctagtc cagacctcc tgggggcag gcggccggc ctgcaagtg gcgcgggcc aagcaccgc gtcttgcaa atcctcagc aattggccg ctgttcggc agcagcttc ttcgccgtg aagcgcgtc tcgggcgac ttggagaac tggcaagag catattctg gcgctgaac cgcagcggg gtacacttt ctggacatg accagtcac gagacgctg cagcattgg ccgttcgcc gaggtgatt tcgaccagg aaggtgcgg tccgaggac ggggccctg tttttggat atgaagtgt gggaatttg ttgcagcaa cgagtgacg aggttgcag actgaacaa gcgcatgag atttccagg ctggttagg cagtatatt aatctggag caggcgagc agcgcgagg aattag
- >TC006465 RA
ccgataata aacagtggc gataatgga gagtgactg taacaggtg cttcaggtc ccattcttc ttatttcag gtaaaatcc cccattcga cgataaaat ggagaccat cagccacgt tcaacactg ctagtttct atacgcata ccaacctaa cgggacgca cctttactt cacctctcc aacgacccg acagtgttg ttgagttac ctgaagtgc tgagtgtga taacttgtg cgaaatcta acgaaatga agtgattct ttcagtgcc aaaatgttc ggataatta aaaaatatc gcaacggat ttttcgaaa atgtttgag aggaaatag ccatggagt ggagtaccg gcgacctga tctggtttg acccaggag tgggccact ggctccctg gcgaggtcc tagaatgtc acaggagtg ccaacgtgt taaccgtcc aagcagtca tcaacggga agccgcaga cgttcgctc tgacggagg gcgagggct cggtccggc gccgccagg acctgggcc ccggcggcg tcgaggaca tgatccaac tcaccgact tgcacgaag ccgccttat tatggaatc tcaagctgc ggtacgacc ggaacttga tctacacgt acgcgggaa gtattctcg tggccgtta acccctaca ggatgttcg actccagct acggcatcg aagcggcgc agagataca ggggcaaga tgatcggcg ctttgcctc cgcatctct tcgccttgg gcgcttccg cctactcgg cgctgcccg ccccccagg tggtggtga tcagcgggg agagcggct ccggaaaga ctgaatcca ccaaactcg tcatgcagt acttggcgg cggtggcgc cgtccgccc ccagaggac aggccttgg tcacggagc agatcctcg aggcggctc ccttgcttg aagctttcg ggaatgcgc gaacggcac gcaatgata acagttcgc ggtttggga agtacctcg aggtttatt tcaagtatg gggcgatcg tcggggcta aagtgactc agtaccttt tggagaagt cgaggatta tcactcaag cgccgggcg aacggaatt atcacgttt tttacgaac ttttgggcg gtttgagta acacagaaa gacagaagt acggtttag tcgacgctg ataagtact tctatctta accaagggg gcggcgatt gtgcgccgg gtcattccg gctcggggg ccgactggg gggctctga caagagcca tgcaagtgt taggtgtcg gagaggcgg aacaagaag gaataatta aggttctag cctcggttc tgcacttgg gaaatgtct atttccata gaaggcagc tccggcatg gccaggagg gtgtggaag ttgggtccg atgttgaaa ttaagtggg ctgctcatc ttttgcaaa tttccccca gtggtttac aaagagcct tgacttcaa gaatcacag aagctagag ccgaacgcg tctactcac ctttaagta tagatcaag cgctagacg cgagagatg ctttcgcca aagctctgt attctgccc ttttcaatt ggttagtga cgcgtgtca attcaattg tccagcgag gaggactac atgatgcgg cccgaattt cgctacttg acattttcg ggtttgaga atctaaacg agaattcct tcgaacaat tatgcatca actatgcct cagaatcgt tgcaattgt atttcaaca aacacgtct ttaaactgg aacaacagg agtatgcaa gggagcgtc tagaatgga ccaacatga cttggatgg acaacacgc cagtcatac atctcttgg gcaaaaaac cagtaggta ttctccacc ttttagacg acgaaagca acttcccca gagcttccg atgcttcgt ttttggaaa agtgtcact ataatcacg ccctgaacg agcattatt gccgtccga gagtcggag gccgggaat ttgggatta gacacttcg cgggacaag tgtggtatt ccgttgacg gtttcctgg acaagaacc gggacgctt tgcgtccgg aagtggtcg agttgattt catcaagca aggaaccgt tggttgcgt ccattgcca agcctttaa tcaaccaaa cgcaaagtc gtacccttc cgaagggca ccgatggcc gttttgtca caatgaaac ccagaaccc cgacagttg cggcccgct tcagtgaca gtcttcagc aactgttag agtccatgg ctcggtgca acccttggt tcgtccggt gcatcaaac ccaacaacg acaagagcc ccatgcggt ttgacatgc cggtggttc tcgagcaac taagatatg caggaatgt tggacacta ttaaaatcc gccagtcgg gatatccgg tcaggatga agtttcagc aattcgttg agagatata ggtatttac tttctggcg tgttgccaa gaggtgcgc cgtatagag acttatgtc gggccattc tggaacaaa tgccttcca cgggagctg aaggtccag attaccaat taggagcaa cccgcgtct tcctccgag aaaacctcc aacgccagt tggaagtga aacgctccg actgtctcc gagactcgg ccatcgtga tccaaaaac acatcagag ggtacttgg cgcgcaaaa actacaaga acctccgcc gcagtaccg taacaatcc agaaacatt ggcgtggtt acaaacacc gcaaacaat tcaaaacaa tccgacatg gagtcatca aagctcaag ctcttgtcc gcggacgca gagaacgca agcgcttcg cgcaaagaa aagccgact tcaagcgac gcgtcgagg ctgaaaaac tggctaaag aacgtgcta aacaaagag ccgcccgag aggcacaac tacagaaag ctgcaccca aatcaagcg tccaccatc tggaaattc cagcagaac tggctttca ttttctcaa aaatcgaaa acttcaacc cgccgcata ctgaaagaa acctaatca aggttctgg gcggtgtca ctggaacaa tcccgccgc taaacctac cgcatgact tgaaccaat atgagttct cccgatttt cctcggttt acttcaagg gggccgacc tcgggatgc ggaaagaac caatcactg cacccttcc tgtccaagg ctgcgacta gagaccagg attttcaag acgcagtgg cgctgttta aactgattt tgcgctggt ctaacgacg ggcagttga gcggaccgc gcgagcggg cgcttgccg attttatcg tgtacaagg cgctgaatt cgagaggat tgagagacg agttgttgg tgcagttgt gtaaccaag tgtggcgga atgagaatt cggaccgga tttggcaac tgatggcgc attgtctca gttgtttcc agccgagcc cgccattga gcaagtatc tactaaaat tcataaccg accacgcgt ccgccaatt acaaagaat tgctgcaaa ggaaattga cgcgaagtt tgcagaaag caccacatg ctcgctccc ttcctccga ctctcctcg aatggcgca gtacccgcc agcggacga acacagccc tacctttaa ctctccctg ataataacg tggcttcgg tcaatgttg actcttgga cgacttgcg aggaagccg catctatcg ccataaact cgctgggct taaccgccg aaggctgga gcgttgtga tggacgatg ccggtatag tctcagaag gaagtggct tggactaca tcatggacc tcgtatccg aactagaat tatgccccg cttttccca tatctaaat cctctctcc ttaaagtcg gtactcgca gagcatcac cggccgaaa tcgatcact cacccacga ccccaaccc ggccccaag taccaccac cggaacccc caactcacc tcaatcgga aaatatcac gggaaatgg cccgccaat caccccctc accacaagt cgaaccaag tggcctccc ggaaagttt cccgtgaga gtcaccgtg agtactcac ctccggtta ctagtaccc cagctagga aatcctcac atgaggcgc tttctcgca attctgccc tgaacgaac gatactttg atatagaga aagtgaggt ctcggagtt tagataatt tgttgagtg agcctgaac cgaccccgc tctccgatt tgggtttga gtcagtcga gactgaacg accggtacc actctgttg agcagttgg cgccgatta agccagttt tggccaatc agaattaca tgtcgaagc aagagattg attttgagt acccggacg tgtccagtg ttagtacct cacaccggg gaggtccga ggtacatca agtcacaat acaccggca agaaggcac ctccagggt cacactcga gccgtgcac atatcgaga aatcagaat ttggagtta ggagttcgg caatgtctg atactagcg aaactccga gtttggcaa gtcatgtta gaagagtga gggttccaa gccaggcgt ccgacgttg accagtttc tggacgagt tgttcagtc cagtccttg acgggaatt tggacgagt taagcgatg ctcgttccc tggctgcta gtatcaaag gtgggacac gggacgact attccgatt ttctcgacg atgttttgg gggattctc ttgacgaat atttggata tgtctaatg ctgggttca ttgcaagga aaataaagg gtggggggc accggaacc gacgtgaca gtcaaggta gttccgttc tggatcagg aagttgaga aattgactt ccggtactt cacaaccaa acacagcca ggacttcgg ccgataaag gcaatgttg atgattaca ttagcgatt tgtttagac cgattttta taaacgata gtttgaaaa acctgactg agaaacaca atttggtcg atagtatca aaggtgggg gtactaccc atcaacaaa acggtgctg gtccgagtt acacattcc cgaccattc ccgcaccca tagtgggct caccaccgg tacttatgc ccttgttaa gtcccaccc aggagggtt tcatgcctg tttacaacg tcccacctc cgaacggta ccgacatgg ccacatacc aacaaagcc tccagcgcg ccttcctcc agtcagcaa tggcccaga acatccaaa tccaacaac aactcctag cgcaaaacc aagccttgc aacaactac taacccaac aaatcccaa ccgaagtca aagtcaacg aagccatac aaaccaccg ttaaagccc aagtccatc aatcatcac ccaatcgta aatcaagct tcaagacgt ctaacagta cccggaaga gcagttctc caagcacaa acgattaca aatcacgca aaaccagct ccgactcta acatgagca tgcggaacg gtgttccac ccccgcccc cgatgccgc cgccactcg acggaaccg accccagcg aagcccgcc cgtttctgg acccgtatg gacgcgcca agaccgtcc gaatcggca aatggcggt ggccgcccc cgaaagacg aaaaagtgg ccgaaaacg gggaggatt tcatgcagt ttaagatgc gccagaacc agaggaaag tgacaccgg ttagggacc aaccgattg tgacaaacg gacacccaa aagtggagc agtcgtcgg cagaatggg aagaaatcg agttcgaac ccatcgtgc gagagccgg aacggccga ctaaagtga gttcgaagc gatctttcg agattggcg cctccaggc catcgccag gcagcgtcg gcaaactca aactcagtt ctgaaatgc gccaaaggc tcgaaaaag tcactgcga accactcgg tgcgctcga cgagtagtc acgtggaaa aaccagccc gggctgtta acaaactgg aagatacgc ggaaaatga tgctggagc aacagctag ccgggaggt ggggcgacg actctcagg acttaccca gcgggaagt caagtccag tcactccgc ctcatatta gtcacaacg ggagcacct ccccgaccc agcaaagct ggtcgagtt ccaactgga aacctggcc ctccccccc ctccaatcg ggcccaact cgcttcccc cagcccctc ccgggccgg ctccgcccc ctcccgtgg tccggccca atcaacccc cactcccaa tcgagccag tgcgtgaat ccttcatgg cccagcgcc aagaccggg acacttttg gcgtccacc aaaaccgag ttttacaga ataattcca aaaggaact cgtttagtg ccaattggg aggtacaga gtgccataa cgcatgaaa ctcaagacg atagcaccc attgggcta aggatgcac tagatttag acaaaacag atgggcggt tgtcgcgag acagctggg accaagctg actcaacaa cgattactt cgagcgatc aaccgcgaa ggattataa gtgaacgtt gggaaaccg aaaggaaga gagttggga aggaaacag gggagagac cgacgttca gaacacacc agtttaata aaagcgcac aagagaggg agaagaagc attcgattg cgtcgacgc aaatgacgg acaagacgg accgaaatg acgtgccag agtggcccg aattcatgc ggcccatcc acacgccgc ccccgaaat cgcctgtga tccaccgga gccaggacc aggtccaaa cccaatccc ccaagacaa cgcaccgac tcctgcctt tgggcgcct cggcttgtg tgacctaca accgggtat cgtggcttt tgcgcgtga gaaaagaag ttttctccc cgaacgaag ctctcggcc cgccttcaa tcctccact tgatctttt gccaaatcg ttggcgacg tctacggcc tcacccctt gtctccgac tcacccaaa cggaaaaac gcgctggcg ttaacatgt tatcagggt acggcataa ccgccgaaa actacaata gttcccatc gggccaaca tcaagcgaa acgtgatcg agttggccc gcacgtggc ctttgtact tcgcccgct tgttcccag tttcagggg cggcacagt tatcggacg tccaactcg tggctgtgt cccactggg ggatccact tggtcaaaa gggagcaga cccacctcc aggtgctca aatcgttcc ctttgaaag acatcgcct cctgcaccg ccccccgac ccaccaccg tcagtttcg acggccccc agggccggc tcagcctcc acacccctc gggcccaac aactgtccg aaatggtga caaagtact gcgctgaga atcgcaaga gctcatcta gcccgcgct cgccagttg ccggcaagt cattcgaaa cgcagccac ggtaccgtc gacactgcg atgttgcgc ttctttccg gaaaaatga acaagacaa agtgggccg aaatctcga aatacaagc cctccgacg acttccggg ccaactcct tggacagct tggccaact cctccaacc cgtcggaga gcctcagcg acgactcgc acgactcgg actcgggca ccgacacca agtccaaga actccttca cggtcaagt gcgaggtct acgcgcaga acccctgcg ggagcatcc gctcctgga ccaagtcgg agagcagca actcggacg ggtacgatt tcgtcgcca gtgactcgc taggggaga agaaacaag atgttatct cgtcgcgga tggagacga tcccagagg aagcggaac cgaaagtct cagtcaagg agattctag cgcggtttg agaatttga aggataagg atggcaagg attgcaaca acaatcaag tgaagagta atggaatta ccaacaata ttaaaaata tcgccaatg gggccacca agaatatta agaatacgg tggttaata aaagtgaag cgagtattg tcagcgcta aaccgaagt ctgataaca gcgtcgttg aaaaaaatt gtgtgaaca gtgctaaca aaactaacg ctagtaaaa caaatgtta ataaaagta aaaagactg aaaatactg agaccagtg ccaccgcgc ctgcgaaac ctcagacca aggagttac aaatgaaat tatcgccgc cgcagagag taccaccag tcgaacagc gcagcgaag tttcgccaa ggaaggcca acaatgtgc cggaaacga gactcgccg cctctccga ccgagatga gaggcgaaa tgaggggcg aaagggcgc agtccccca gcagccctc tgcccccga ccgggaagt actccctga tgcagttcg ccatgcaca acttcaggc agacggccg atttggagc tcctcaaga tggactctt cgatcaaat ccaaagaga agcgcaaag aatggacct ggaagcagc aaaccgacc tgatcaagt ggcaaggga cgcccatcg acggccctc tgctgcgcc tggaggacc ccgagctgg gccccctgg ccgttgagt gcttcgagt gcattctgc ggtactgcg gggacctgc ccctaaccc cggaaatgc ccgaagtca agtgtgtct acacagtgc tgatgcact gccacaaac acgcccaat tacgtgacg aagtctact gtcagctca tgaagcaga caactagta ataaatccg agtcaccag aatcctgcc aacgcgctt ggagactcc tttcaatcg ttgcagcgt actttgctt gctcggaca atctcctac cgtttttga cggaacacc tctccagcg ccgcttcgg acagacgtc gcgtctgtc atggcactg cggccgtct gccttgcaa acctccgaa agacccagc ggtgtggag gccgcaaga acgtccctt ccgtcgagg aagtgacag cggttagcg ccggacgca gtgcccgcc gccaaatct accgcctcc ctgggggag ccgaacgcg tagtcaaca caagatgta gcacggttg tggccgacg taatcgccg aactgtgca gtctgatcg gcgtcggca ccgaagccg agcaacaag agttcagtt tgtactgca tagtgcaag gggacgcct tcaccatgc ctctcgccg ccgatgagt acatcctgg acgtcacca ccgagctgc acaaagccg cgcagccct tctacctga ttttctgcc gctcggtct ggtaccacc ccctcaaac acgatgcta gtcccctct acaccgaag tcctgttca accaagtgg cccccgact acctcgaag gcctcctcc tgcgcctag gcaacccca acctccccc ccagcgtca tccgcgaca tggccctca ttgccgcgc tcctgcacc gcgccgccg acctgaacc accccccga gcctcaagg aagtcaagt ttctgctcc cgaagcccg tcctgggcc tgcgggagc cccggccgc cgcagtggc tggctctag tccagacct cctgggggc aggcggccg gcctgcaag tggcgcggg ccaagcacc gcgtcttgc aaatcctca gcaattggc cgctgttcg gcagcagct tcttcgccg tgaagcgcg tctcgggcg acttggaga actggcaag agcatattc tggcgctga accgcagcg gggtacact ttctggaca tgaccagtc acgagacgc tgcagcatt ggccgttcg ccgaggtga tttcgacca ggaaggtgc ggtccgagg acggggccc tgtttttgg atatgaagt gtgggaatt tgttgcagc aacgagtga cgaggttgc agactgaac aagcgcatg agatttcca ggctggtta ggcagtata ttaatctgg agcaggcga gcagcgcga ggaattagt taagtgtag gtttaggtt gtatagtca ttgttaact ttgctcact tatccgtgt tactccctt atacgtaat ggaattctg gtaattgtg gaacggaaa tttcgaggt ttgctgttg gccagtaaa gtcgaaagt ggttgttca catggggtt aattggtgg tttcacaat tacttcatt atttattgt tcataccca gaaaagtac gcagtgtat attatttga tacttaatc attttgcga ctgttttag ctataaata tatctttta ttgtcttca ttctgattc aacaaaaag tttcttttg ttgcatggg aattcgttt gttaagtaa tatgtaatt atgagaaaa cattattgt ttttataca cttgatgtg cattatgga aggccggat ttgattatt tattattga gatacgagt taaatctct cgagattga ttcatgtct gacattggc ggcggcctt tgcggaata ttctgtacg taaaattat tggtgtttg tattttcta ttaataaaa gtattttat tgaa
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