Status
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Gene information
General information
TC005686 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9261086..9263633
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC005686
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02903

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 2


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005686 RA
      MSQKMRDIP IAKLNNGIE IPVFGLAAP QVENVQLVV 
SSNYRLMDC SIFENEKRF GVALQQLIE GGKIRRKDL 
FLMSKFCCT NTTNLVKAA LLDTLKKLQ VAYLDLYLI 
ERPPACKDH NLIETWEAM EGLYSEKLT RAIGLSNFN 
SKQIERILE NCTIRPQVN QIQCHPYLN QAKLRKYCS 
NNGIAVISY SPLGARNNI QPESLDVLS DCRIKEIAR 
RYNKTPTEI VLKYNVQLG NIVISDMLI ESANFFDFY 
LAPEDMSYI STFSSESLK NLQLDYLDV YLMHWPHAL 
KEGPSLHPI DPKTGLFIP SDVDFVDTW KAMEKMHEK 
DLTKSIGIS NFNSNQIDR LLKSAKIAP VINQIECHP 
YLNQSKLRK FCSDRGITV TSYSPLGSP ARPWQKPGD 
PYVINDPKI KEIGNKYGK SPAQILLRY NVQLGNVVI 
PKSSNRNRL VENMSIFDF TLSEDDMKY IGTFDCNGR 
ICPQAEARE HPFYPFKPG VEF
    CDS sequence
    • >TC005686 RA
      atgtcacaa aaaatgcgt gatattcct attgctaag 
ttaaataat gggatagaa attccagtt tttggatta 
gcggcacct caggtggaa aatgtgcaa cttgtggta 
agttcaaac taccgacta atggattgc tctattttt 
gaaaatgag aaaagattt ggagtcgct cttcaacaa 
ctaatcgaa ggaggaaaa atcagacgg aaagactta 
tttctcatg agcaaattt tgctgcact aacaccaca 
aatttagtc aaggctgct cttttagac actttgaaa 
aaattacaa gttgcatat ttagattta tacttaatt 
gagcgacca ccagcttgc aaagaccac aaccttatt 
gaaacgtgg gaagctatg gaagggctt tacagtgag 
aaactgacc agagccatt ggtctttca aattttaac 
agtaaacag atcgaacgc attttagaa aactgcaca 
attcgcccg caagttaat caaattcaa tgtcaccct 
tacttaaat caagctaaa ttacgaaag tattgttcg 
aacaatgga attgctgta ataagttac agtccgttg 
ggtgcgagg aataatatt caacctgaa agtctcgac 
gttcttagt gactgcagg attaaagaa attgcaagg 
agatacaac aaaacacca acagaaatt gtgctaaaa 
tataacgtg cagttagga aatattgtc atttcggac 
atgttaatc gaaagtgcg aattttttc gatttttac 
ttagcacct gaagatatg agttacatt tcaacgttt 
agttcagaa tcgctgaaa aacctccag ttggactat 
ctcgatgtt tatcttatg cactggcca catgctctc 
aaggaaggc cctagtttg cacccaatc gacccaaaa 
acaggccta tttatccca agcgatgtc gattttgtc 
gacacttgg aaagccatg gaaaaaatg cacgaaaaa 
gatctcacc aaatcgatt ggcatttcc aacttcaac 
agtaaccaa atcgacaga cttttaaaa tcggccaaa 
atcgcccct gttataaat cagattgaa tgtcaccct 
tatttgaac caaagcaag ttgcgtaaa ttttgctct 
gataggggc attactgta acttcgtat agtccttta 
ggatcccca gcgaggccg tggcaaaaa cccggcgat 
ccctatgtc attaacgac cctaaaatc aaggaaatc 
ggaaataag tacggaaaa tcacccgcg caaattctg 
ctccggtat aatgtgcag ttaggaaac gttgttata 
ccgaaaagt tccaacaga aatcggctt gtggaaaat 
atgagcatt tttgatttc acactgagc gaggacgac 
atgaaatac atcggcact ttcgactgt aatggcagg 
atttgtcca caagctgag gccagagag cacccgttc 
tatcccttt aagcccgga gttgaattt taa
    mRNA sequence
    • >TC005686 RA
      ataattaat ttcattgat ttttccgac cgtgcactc 
taaaaatgt cacaaaaaa tgcgtgata ttcctattg 
ctaagttaa ataatggga tagaaattc cagtttttg 
gattagcgg cacctcagg tggaaaatg tgcaacttg 
tggtaagtt caaactacc gactaatgg attgctcta 
tttttgaaa atgagaaaa gatttggag tcgctcttc 
aacaactaa tcgaaggag gaaaaatca gacggaaag 
acttatttc tcatgagca aattttgct gcactaaca 
ccacaaatt tagtcaagg ctgctcttt tagacactt 
tgaaaaaat tacaagttg catatttag atttatact 
taattgagc gaccaccag cttgcaaag accacaacc 
ttattgaaa cgtgggaag ctatggaag ggctttaca 
gtgagaaac tgaccagag ccattggtc tttcaaatt 
ttaacagta aacagatcg aacgcattt tagaaaact 
gcacaattc gcccgcaag ttaatcaaa ttcaatgtc 
acccttact taaatcaag ctaaattac gaaagtatt 
gttcgaaca atggaattg ctgtaataa gttacagtc 
cgttgggtg cgaggaata atattcaac ctgaaagtc 
tcgacgttc ttagtgact gcaggatta aagaaattg 
caaggagat acaacaaaa caccaacag aaattgtgc 
taaaatata acgtgcagt taggaaata ttgtcattt 
cggacatgt taatcgaaa gtgcgaatt ttttcgatt 
tttacttag cacctgaag atatgagtt acatttcaa 
cgtttagtt cagaatcgc tgaaaaacc tccagttgg 
actatctcg atgtttatc ttatgcact ggccacatg 
ctctcaagg aaggcccta gtttgcacc caatcgacc 
caaaaacag gcctattta tcccaagcg atgtcgatt 
ttgtcgaca cttggaaag ccatggaaa aaatgcacg 
aaaaagatc tcaccaaat cgattggca tttccaact 
tcaacagta accaaatcg acagacttt taaaatcgg 
ccaaaatcg cccctgtta taaatcaga ttgaatgtc 
acccttatt tgaaccaaa gcaagttgc gtaaatttt 
gctctgata ggggcatta ctgtaactt cgtatagtc 
ctttaggat ccccagcga ggccgtggc aaaaacccg 
gcgatccct atgtcatta acgacccta aaatcaagg 
aaatcggaa ataagtacg gaaaatcac ccgcgcaaa 
ttctgctcc ggtataatg tgcagttag gaaacgttg 
ttataccga aaagttcca acagaaatc ggcttgtgg 
aaaatatga gcatttttg atttcacac tgagcgagg 
acgacatga aatacatcg gcactttcg actgtaatg 
gcaggattt gtccacaag ctgaggcca gagagcacc 
cgttctatc cctttaagc ccggagttg aattttaat 
acgttttgt tttgtgatt atgtgttac gagttgtgt 
aatattgac aaaaaccaa taaatgata tttatagaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY