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Gene information
General information
TC005686 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 2197856..2200403
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005686
    iBeetle screen iB_02903
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 2


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005686 RA
      MSQKMRDIP IAKLNNGIE IPVFGLAAP QVENVQLVV 
      SSNYRLMDC SIFENEKRF GVALQQLIE GGKIRRKDL 
      FLMSKFCCT NTTNLVKAA LLDTLKKLQ VAYLDLYLI 
      ERPPACKDH NLIETWEAM EGLYSEKLT RAIGLSNFN 
      SKQIERILE NCTIRPQVN QIQCHPYLN QAKLRKYCS 
      NNGIAVISY SPLGARNNI QPESLDVLS DCRIKEIAR 
      RYNKTPTEI VLKYNVQLG NIVISDMLI ESANFFDFY 
      LAPEDMSYI STFSSESLK NLQLDYLDV YLMHWPHAL 
      KEGPSLHPI DPKTGLFIP SDVDFVDTW KAMEKMHEK 
      DLTKSIGIS NFNSNQIDR LLKSAKIAP VINQIECHP 
      YLNQSKLRK FCSDRGITV TSYSPLGSP ARPWQKPGD 
      PYVINDPKI KEIGNKYGK SPAQILLRY NVQLGNVVI 
      PKSSNRNRL VENMSIFDF TLSEDDMKY IGTFDCNGR 
      ICPQAEARE HPFYPFKPG VEF
    CDS sequence
    • >TC005686 RA
      atgtcacaa aaaatgcgt gatattcct attgctaag 
      ttaaataat gggatagaa attccagtt tttggatta 
      gcggcacct caggtggaa aatgtgcaa cttgtggta 
      agttcaaac taccgacta atggattgc tctattttt 
      gaaaatgag aaaagattt ggagtcgct cttcaacaa 
      ctaatcgaa ggaggaaaa atcagacgg aaagactta 
      tttctcatg agcaaattt tgctgcact aacaccaca 
      aatttagtc aaggctgct cttttagac actttgaaa 
      aaattacaa gttgcatat ttagattta tacttaatt 
      gagcgacca ccagcttgc aaagaccac aaccttatt 
      gaaacgtgg gaagctatg gaagggctt tacagtgag 
      aaactgacc agagccatt ggtctttca aattttaac 
      agtaaacag atcgaacgc attttagaa aactgcaca 
      attcgcccg caagttaat caaattcaa tgtcaccct 
      tacttaaat caagctaaa ttacgaaag tattgttcg 
      aacaatgga attgctgta ataagttac agtccgttg 
      ggtgcgagg aataatatt caacctgaa agtctcgac 
      gttcttagt gactgcagg attaaagaa attgcaagg 
      agatacaac aaaacacca acagaaatt gtgctaaaa 
      tataacgtg cagttagga aatattgtc atttcggac 
      atgttaatc gaaagtgcg aattttttc gatttttac 
      ttagcacct gaagatatg agttacatt tcaacgttt 
      agttcagaa tcgctgaaa aacctccag ttggactat 
      ctcgatgtt tatcttatg cactggcca catgctctc 
      aaggaaggc cctagtttg cacccaatc gacccaaaa 
      acaggccta tttatccca agcgatgtc gattttgtc 
      gacacttgg aaagccatg gaaaaaatg cacgaaaaa 
      gatctcacc aaatcgatt ggcatttcc aacttcaac 
      agtaaccaa atcgacaga cttttaaaa tcggccaaa 
      atcgcccct gttataaat cagattgaa tgtcaccct 
      tatttgaac caaagcaag ttgcgtaaa ttttgctct 
      gataggggc attactgta acttcgtat agtccttta 
      ggatcccca gcgaggccg tggcaaaaa cccggcgat 
      ccctatgtc attaacgac cctaaaatc aaggaaatc 
      ggaaataag tacggaaaa tcacccgcg caaattctg 
      ctccggtat aatgtgcag ttaggaaac gttgttata 
      ccgaaaagt tccaacaga aatcggctt gtggaaaat 
      atgagcatt tttgatttc acactgagc gaggacgac 
      atgaaatac atcggcact ttcgactgt aatggcagg 
      atttgtcca caagctgag gccagagag cacccgttc 
      tatcccttt aagcccgga gttgaattt taa
    mRNA sequence
    • >TC005686 RA
      ataattaat ttcattgat ttttccgac cgtgcactc 
      taaaaatgt cacaaaaaa tgcgtgata ttcctattg 
      ctaagttaa ataatggga tagaaattc cagtttttg 
      gattagcgg cacctcagg tggaaaatg tgcaacttg 
      tggtaagtt caaactacc gactaatgg attgctcta 
      tttttgaaa atgagaaaa gatttggag tcgctcttc 
      aacaactaa tcgaaggag gaaaaatca gacggaaag 
      acttatttc tcatgagca aattttgct gcactaaca 
      ccacaaatt tagtcaagg ctgctcttt tagacactt 
      tgaaaaaat tacaagttg catatttag atttatact 
      taattgagc gaccaccag cttgcaaag accacaacc 
      ttattgaaa cgtgggaag ctatggaag ggctttaca 
      gtgagaaac tgaccagag ccattggtc tttcaaatt 
      ttaacagta aacagatcg aacgcattt tagaaaact 
      gcacaattc gcccgcaag ttaatcaaa ttcaatgtc 
      acccttact taaatcaag ctaaattac gaaagtatt 
      gttcgaaca atggaattg ctgtaataa gttacagtc 
      cgttgggtg cgaggaata atattcaac ctgaaagtc 
      tcgacgttc ttagtgact gcaggatta aagaaattg 
      caaggagat acaacaaaa caccaacag aaattgtgc 
      taaaatata acgtgcagt taggaaata ttgtcattt 
      cggacatgt taatcgaaa gtgcgaatt ttttcgatt 
      tttacttag cacctgaag atatgagtt acatttcaa 
      cgtttagtt cagaatcgc tgaaaaacc tccagttgg 
      actatctcg atgtttatc ttatgcact ggccacatg 
      ctctcaagg aaggcccta gtttgcacc caatcgacc 
      caaaaacag gcctattta tcccaagcg atgtcgatt 
      ttgtcgaca cttggaaag ccatggaaa aaatgcacg 
      aaaaagatc tcaccaaat cgattggca tttccaact 
      tcaacagta accaaatcg acagacttt taaaatcgg 
      ccaaaatcg cccctgtta taaatcaga ttgaatgtc 
      acccttatt tgaaccaaa gcaagttgc gtaaatttt 
      gctctgata ggggcatta ctgtaactt cgtatagtc 
      ctttaggat ccccagcga ggccgtggc aaaaacccg 
      gcgatccct atgtcatta acgacccta aaatcaagg 
      aaatcggaa ataagtacg gaaaatcac ccgcgcaaa 
      ttctgctcc ggtataatg tgcagttag gaaacgttg 
      ttataccga aaagttcca acagaaatc ggcttgtgg 
      aaaatatga gcatttttg atttcacac tgagcgagg 
      acgacatga aatacatcg gcactttcg actgtaatg 
      gcaggattt gtccacaag ctgaggcca gagagcacc 
      cgttctatc cctttaagc ccggagttg aattttaat 
      acgttttgt tttgtgatt atgtgttac gagttgtgt 
      aatattgac aaaaaccaa taaatgata tttatagaa