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Gene information
General information
TC005740 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 1207491..1212290
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005740
    iBeetle screen iB_02901
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005740 RA
      MKTLTAFAA VICISLADD TALKEFTSG NNLFTTSIY 
      KEIKKTTPG NFLVSPFSA ESVLALVQG GAKDATALE 
      MRDALHLPA APETTQAAI KALHPQLKQ TLYTLKTAN 
      KIYLKPGFE VRPEFRKVA SEVFEAGLE NIDFERKEE 
      AAQAMNQWV EEQTEHKIQ NLISPENLN SRTRSILIN 
      ALYFKGNWS SPFKKFLTK EEDFYKNGK QVAPVATMK 
      LQSSTKYYE SEELKAKFL ELPFEGGDV SMIIVLPNE 
      KDGLEVLEN QLEKTVELP QLAEEYVKV ALPKFQIRS 
      QFILKEMLQ ELGMKKAFK GEEADLSGI AGDKGDLVI 
      DQVVQKTFI DLNEDGVEA AAATFVGVG IPLSGVVST 
      VTPKEFKAD HPFLFYIKF KDLILFAGR VVDPTDSK
    CDS sequence
    • >TC005740 RA
      atgaagact ctcacagcc ttcgccgca gtaatttgc 
      atctccttg gccgacgac acggccttg aaagaattc 
      acttcaggc aataatttg tttaccaca tcaatttac 
      aaagaaatc aaaaaaaca acacctggc aatttcctc 
      gtgagcccc ttttcggcc gagtcggtc ctggccctg 
      gtccaaggg ggcgccaag gacgcaacc gccctggaa 
      atgcgcgac gccctccac ctgcccgcc gcccccgaa 
      acgacccaa gccgccatc aaagcactc cacccccaa 
      ctcaagcaa accctctac accctcaaa accgccaac 
      aagatctac ctcaaacca gggtttgag gtgcggccc 
      gagttccgg aaagtggca agtgaggtt tttgaggcc 
      ggcttggag aacatcgat tttgagagg aaagaggaa 
      gctgcccag gccatgaac caatgggtg gaggaacaa 
      actgaacac aagatccag aatttgatc agtccggag 
      aatctcaat tccaggacg cgctcaatt ttgatcaat 
      gccttgtat ttcaagggc aattggagc tctccgttt 
      aagaaattt ttaacgaaa gaggaggat ttttacaag 
      aatggcaaa caagtggct cctgttgcc acaatgaag 
      ctgcagtcg tcgacaaaa tactacgag agtgaggaa 
      cttaaggcc aagtttttg gagctgccg ttcgaaggg 
      ggtgatgtg tcgatgatt attgtgctt ccgaatgag 
      aaagacggg cttgaggtt ttggaaaat cagttagag 
      aaaaccgtt gagttgcca caactggcg gaagagtac 
      gtgaaagtg gcgctcccg aaattccaa atccggagc 
      caattcatt ttgaaggaa atgctacaa gagctgggg 
      atgaagaaa gccttcaag ggcgaggag gccgacttg 
      agtggcatt gcaggtgac aaaggcgat ctcgtaatt 
      gaccaagtc gtccaaaaa actttcatt gatttgaac 
      gaggatgga gttgaggca gcggctgct actttcgtt 
      ggagttgga attccttta agtggagtt gtttcaact 
      gtcactccg aaagaattc aaagccgac catccgttc 
      ctgttctac atcaaattc aaagacttg atcctgttc 
      gctggacgg gtcgtggat ccgaccgat tcgaaataa
    mRNA sequence
    • >TC005740 RA
      cgctttgtt cgtaaccac cttttaacc aagtgctgt 
      tttgcaact cgttcagtt ccaaagatc catccaaac 
      aatgaagac tctcacagc cttcgccgc agtaatttg 
      catctcctt ggccgacga cacggcctt gaaagaatt 
      cacttcagg caataattt gtttaccac atcaattta 
      caaagaaat caaaaaaac aacacctgg caatttcct 
      cgtgagccc cttttcggc cgagtcggt cctggccct 
      ggtccaagg gggcgccaa ggacgcaac cgccctgga 
      aatgcgcga cgccctcca cctgcccgc cgcccccga 
      aacgaccca agccgccat caaagcact ccaccccca 
      actcaagca aaccctcta caccctcaa aaccgccaa 
      caagatcta cctcaaacc agggtttga ggtgcggcc 
      cgagttccg gaaagtggc aagtgaggt ttttgaggc 
      cggcttgga gaacatcga ttttgagag gaaagagga 
      agctgccca ggccatgaa ccaatgggt ggaggaaca 
      aactgaaca caagatcca gaatttgat cagtccgga 
      gaatctcaa ttccaggac gcgctcaat tttgatcaa 
      tgccttgta tttcaaggg caattggag ctctccgtt 
      taagaaatt tttaacgaa agaggagga tttttacaa 
      gaatggcaa acaagtggc tcctgttgc cacaatgaa 
      gctgcagtc gtcgacaaa atactacga gagtgagga 
      acttaaggc caagttttt ggagctgcc gttcgaagg 
      gggtgatgt gtcgatgat tattgtgct tccgaatga 
      gaaagacgg gcttgaggt tttggaaaa tcagttaga 
      gaaaaccgt tgagttgcc acaactggc ggaagagta 
      cgtgaaagt ggcgctccc gaaattcca aatccggag 
      ccaattcat tttgaagga aatgctaca agagctggg 
      gatgaagaa agccttcaa gggcgagga ggccgactt 
      gagtggcat tgcaggtga caaaggcga tctcgtaat 
      tgaccaagt cgtccaaaa aactttcat tgatttgaa 
      cgaggatgg agttgaggc agcggctgc tactttcgt 
      tggagttgg aattccttt aagtggagt tgtttcaac 
      tgtcactcc gaaagaatt caaagccga ccatccgtt 
      cctgttcta catcaaatt caaagactt gatcctgtt 
      cgctggacg ggtcgtgga tccgaccga ttcgaaata 
      aatagtgtt gtgaaaaat aaagtgtaa taataaata 
      ttaaacaat ggaaactta tgcaataaa caatgaaat 
      aaaaa