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Gene information
General information
TC034047
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005809 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7399050..7404700
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034047
    iBeetle screen iB_02899

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034047 RA
      MDTGLPQPS RSESDELRT PRRLIPQRY VVTLMILLV 
VINSYANRD TLMYTIQYF AEISQKKHL YKNVTIGQH 
CEILQDNYH KTSSLVQNT TTKLVKLKQ SSVGTINAI 
LFVSYLPTH ILGGIYSDS RSPKRVISI STLVTSVIN 
ICTPVLISA TKSNLTVLK VLRLLMGIS QGAFLPAST 
TLMAHWAPV RERASISTI SVSGLYIGL ICSKICTSI 
LVESSGSWS TPYYSKRPT VPTVPCVVG EIVDHRRKD 
IPYRAILTS FHVWVLIVA FIAYRWIGN FFAFTLPEY 
LEVVLKYNE RESLLISSL PFLPITVIL ILSGFLSDW 
IVRNGYINL ITVRKVFST IGMMGPPIY MLTGSFASC 
NRFAAVVLF VLALSLMVF TFLGLYLIT LDLGPNYAG 
FTTSLTNGL GNISWVFLN KVKDIIAPD KTVLQYRKL 
FWLSMVIAV VTNTIFMIF GTTKVQAWN KPLVKGK
    CDS sequence
    • >TC034047 RA
      atggacact ggacttccc caaccctca cgttcagaa 
tctgacgaa ctgagaact ccccgacga cttatcccg 
caacgctat gtagtaact ttgatgatc ctactggtg 
gtaataaac agctatgca aatcgtgat actctaatg 
tacaccatt caatatttc gcggaaatc agtcagaag 
aaacacctc tacaagaat gtcacaatt ggacaacac 
tgcgaaatc ctacaagac aattaccac aaaacctca 
tcacttgtg caaaacacg accacaaaa ttggtaaaa 
ctgaagcaa tcgtcagtt ggaacaatc aacgcgatt 
ctcttcgtg tcgtatctg cctacacac attttggga 
gggatttac tcggacagc cgcagcccc aagcgagtg 
atttcgatc tcgacttta gttacctca gtcatcaat 
atttgcacc ccggtgctt atcagtgcg accaagagc 
aatctcacg gtactgaag gtactgaga ctactcatg 
ggcatatcc cagggtgct ttcctcccg gcttcgacc 
actctgatg gcccactgg gccccagtg cgcgagaga 
gcctccata tccacaata tcagtctcg gggttgtac 
atcggactt atctgtagt aaaatttgc acaagtatt 
ttagttgag tcgagtggc agttggtcc actccgtac 
tacagtaaa cgaccaacg gtaccaaca gtaccatgc 
gttgtaggg gaaatcgtt gaccaccgc cgtaaggac 
atcccctac cgtgccatt ctcacctcc ttccatgtc 
tgggttttg atagtggcc ttcatagcg tatcgatgg 
attggcaat tttttcgcc ttcaccctt cctgagtac 
ttggaggtt gttctcaag tacaacgag agggaaagt 
ttgctcatt tcctcactc ccgttcctc cccataaca 
gtcattcta atcctgtca gggttcctg tcggactgg 
atcgtccgg aacggctac attaacctg ataacagtc 
cggaaagtc ttctccacc attggaatg atggggccc 
cccatttac atgttaacc gggtcattt gccagttgc 
aaccgattt gcagccgtg gtcttgttt gtcctagct 
ctgagtctc atggttttt acttttttg ggactttac 
ttaatcacg ctggatttg ggaccaaac tatgcgggg 
tttacgaca agtctcacc aatggactt ggaaacatt 
tcgtgggtt tttcttaac aaagtcaaa gatattatt 
gcaccggat aaaactgta ctacagtac cggaagctc 
ttttggttg tcgatggtt atagctgtg gtcacgaac 
acgattttt atgattttt ggtaccacc aaggtacag 
gcgtggaac aagcctttg gttaaagga aaataa
    mRNA sequence
    • >TC034047 RA
      cgctcaaat aaatttaat cgcccttta cttgtctac 
aattgccaa gttggctct cgctacttt tgtcaaaat 
gacacgcca aaaatggac actggactt ccccaaccc 
tcacgttca gaatctgac gaactgaga actccccga 
cgacttatc ccgcaacgc tatgtagta actttgatg 
atcctactg gtggtaata aacagctat gcaaatcgt 
gatactcta atgtacacc attcaatat ttcgcggaa 
atcagtcag aagaaacac ctctacaag aatgtcaca 
attggacaa cactgcgaa atcctacaa gacaattac 
cacaaaacc tcatcactt gtgcaaaac acgaccaca 
aaattggta aaactgaag caatcgtca gttggaaca 
atcaacgcg attctcttc gtgtcgtat ctgcctaca 
cacattttg ggagggatt tactcggac agccgcagc 
cccaagcga gtgatttcg atctcgact ttagttacc 
tcagtcatc aatatttgc accccggtg cttatcagt 
gcgaccaag agcaatctc acggtactg aaggtactg 
agactactc atgggcata tcccagggt gctttcctc 
ccggcttcg accactctg atggcccac tgggcccca 
gtgcgcgag agagcctcc atatccaca atatcagtc 
tcggggttg tacatcgga cttatctgt agtaaaatt 
tgcacaagt attttagtt gagtcgagt ggcagttgg 
tccactccg tactacagt aaacgacca acggtacca 
acagtacca tgcgttgta ggggaaatc gttgaccac 
cgccgtaag gacatcccc taccgtgcc attctcacc 
tccttccat gtctgggtt ttgatagtg gccttcata 
gcgtatcga tggattggc aattttttc gccttcacc 
cttcctgag tacttggag gttgttctc aagtacaac 
gagagggaa agtttgctc atttcctca ctcccgttc 
ctccccata acagtcatt ctaatcctg tcagggttc 
ctgtcggac tggatcgtc cggaacggc tacattaac 
ctgataaca gtccggaaa gtcttctcc accattgga 
atgatgggg ccccccatt tacatgtta accgggtca 
tttgccagt tgcaaccga tttgcagcc gtggtcttg 
tttgtccta gctctgagt ctcatggtt tttactttt 
ttgggactt tacttaatc acgctggat ttgggacca 
aactatgcg gggtttacg acaagtctc accaatgga 
cttggaaac atttcgtgg gtttttctt aacaaagtc 
aaagatatt attgcaccg gataaaact gtactacag 
taccggaag ctcttttgg ttgtcgatg gttatagct 
gtggtcacg aacacgatt tttatgatt tttggtacc 
accaaggta caggcgtgg aacaagcct ttggttaaa 
ggaaaataa agagatttc tagggctag cgagtgttt 
catttgtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY