Status
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Gene information
General information
TC034047
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005809
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7399050..7404700
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034047
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02899

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034047 RA
      MDTGLPQPS RSESDELRT PRRLIPQRY VVTLMILLV 
      VINSYANRD TLMYTIQYF AEISQKKHL YKNVTIGQH 
      CEILQDNYH KTSSLVQNT TTKLVKLKQ SSVGTINAI 
      LFVSYLPTH ILGGIYSDS RSPKRVISI STLVTSVIN 
      ICTPVLISA TKSNLTVLK VLRLLMGIS QGAFLPAST 
      TLMAHWAPV RERASISTI SVSGLYIGL ICSKICTSI 
      LVESSGSWS TPYYSKRPT VPTVPCVVG EIVDHRRKD 
      IPYRAILTS FHVWVLIVA FIAYRWIGN FFAFTLPEY 
      LEVVLKYNE RESLLISSL PFLPITVIL ILSGFLSDW 
      IVRNGYINL ITVRKVFST IGMMGPPIY MLTGSFASC 
      NRFAAVVLF VLALSLMVF TFLGLYLIT LDLGPNYAG 
      FTTSLTNGL GNISWVFLN KVKDIIAPD KTVLQYRKL 
      FWLSMVIAV VTNTIFMIF GTTKVQAWN KPLVKGK
    CDS sequence
    • >TC034047 RA
      atggacact ggacttccc caaccctca cgttcagaa 
      tctgacgaa ctgagaact ccccgacga cttatcccg 
      caacgctat gtagtaact ttgatgatc ctactggtg 
      gtaataaac agctatgca aatcgtgat actctaatg 
      tacaccatt caatatttc gcggaaatc agtcagaag 
      aaacacctc tacaagaat gtcacaatt ggacaacac 
      tgcgaaatc ctacaagac aattaccac aaaacctca 
      tcacttgtg caaaacacg accacaaaa ttggtaaaa 
      ctgaagcaa tcgtcagtt ggaacaatc aacgcgatt 
      ctcttcgtg tcgtatctg cctacacac attttggga 
      gggatttac tcggacagc cgcagcccc aagcgagtg 
      atttcgatc tcgacttta gttacctca gtcatcaat 
      atttgcacc ccggtgctt atcagtgcg accaagagc 
      aatctcacg gtactgaag gtactgaga ctactcatg 
      ggcatatcc cagggtgct ttcctcccg gcttcgacc 
      actctgatg gcccactgg gccccagtg cgcgagaga 
      gcctccata tccacaata tcagtctcg gggttgtac 
      atcggactt atctgtagt aaaatttgc acaagtatt 
      ttagttgag tcgagtggc agttggtcc actccgtac 
      tacagtaaa cgaccaacg gtaccaaca gtaccatgc 
      gttgtaggg gaaatcgtt gaccaccgc cgtaaggac 
      atcccctac cgtgccatt ctcacctcc ttccatgtc 
      tgggttttg atagtggcc ttcatagcg tatcgatgg 
      attggcaat tttttcgcc ttcaccctt cctgagtac 
      ttggaggtt gttctcaag tacaacgag agggaaagt 
      ttgctcatt tcctcactc ccgttcctc cccataaca 
      gtcattcta atcctgtca gggttcctg tcggactgg 
      atcgtccgg aacggctac attaacctg ataacagtc 
      cggaaagtc ttctccacc attggaatg atggggccc 
      cccatttac atgttaacc gggtcattt gccagttgc 
      aaccgattt gcagccgtg gtcttgttt gtcctagct 
      ctgagtctc atggttttt acttttttg ggactttac 
      ttaatcacg ctggatttg ggaccaaac tatgcgggg 
      tttacgaca agtctcacc aatggactt ggaaacatt 
      tcgtgggtt tttcttaac aaagtcaaa gatattatt 
      gcaccggat aaaactgta ctacagtac cggaagctc 
      ttttggttg tcgatggtt atagctgtg gtcacgaac 
      acgattttt atgattttt ggtaccacc aaggtacag 
      gcgtggaac aagcctttg gttaaagga aaataa
    mRNA sequence
    • >TC034047 RA
      cgctcaaat aaatttaat cgcccttta cttgtctac 
      aattgccaa gttggctct cgctacttt tgtcaaaat 
      gacacgcca aaaatggac actggactt ccccaaccc 
      tcacgttca gaatctgac gaactgaga actccccga 
      cgacttatc ccgcaacgc tatgtagta actttgatg 
      atcctactg gtggtaata aacagctat gcaaatcgt 
      gatactcta atgtacacc attcaatat ttcgcggaa 
      atcagtcag aagaaacac ctctacaag aatgtcaca 
      attggacaa cactgcgaa atcctacaa gacaattac 
      cacaaaacc tcatcactt gtgcaaaac acgaccaca 
      aaattggta aaactgaag caatcgtca gttggaaca 
      atcaacgcg attctcttc gtgtcgtat ctgcctaca 
      cacattttg ggagggatt tactcggac agccgcagc 
      cccaagcga gtgatttcg atctcgact ttagttacc 
      tcagtcatc aatatttgc accccggtg cttatcagt 
      gcgaccaag agcaatctc acggtactg aaggtactg 
      agactactc atgggcata tcccagggt gctttcctc 
      ccggcttcg accactctg atggcccac tgggcccca 
      gtgcgcgag agagcctcc atatccaca atatcagtc 
      tcggggttg tacatcgga cttatctgt agtaaaatt 
      tgcacaagt attttagtt gagtcgagt ggcagttgg 
      tccactccg tactacagt aaacgacca acggtacca 
      acagtacca tgcgttgta ggggaaatc gttgaccac 
      cgccgtaag gacatcccc taccgtgcc attctcacc 
      tccttccat gtctgggtt ttgatagtg gccttcata 
      gcgtatcga tggattggc aattttttc gccttcacc 
      cttcctgag tacttggag gttgttctc aagtacaac 
      gagagggaa agtttgctc atttcctca ctcccgttc 
      ctccccata acagtcatt ctaatcctg tcagggttc 
      ctgtcggac tggatcgtc cggaacggc tacattaac 
      ctgataaca gtccggaaa gtcttctcc accattgga 
      atgatgggg ccccccatt tacatgtta accgggtca 
      tttgccagt tgcaaccga tttgcagcc gtggtcttg 
      tttgtccta gctctgagt ctcatggtt tttactttt 
      ttgggactt tacttaatc acgctggat ttgggacca 
      aactatgcg gggtttacg acaagtctc accaatgga 
      cttggaaac atttcgtgg gtttttctt aacaaagtc 
      aaagatatt attgcaccg gataaaact gtactacag 
      taccggaag ctcttttgg ttgtcgatg gttatagct 
      gtggtcacg aacacgatt tttatgatt tttggtacc 
      accaaggta caggcgtgg aacaagcct ttggttaaa 
      ggaaaataa agagatttc tagggctag cgagtgttt 
      catttgtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY