| General information | |||
| TC006336 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 7370899..7376218 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006336 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02898 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC006336 RA
MIEFLATFG LFYLTVGLL PLLVFSYFV LSLSHKLWI FTISILHPD VEFLRTITV RTLLDTHRN QGIISVLLC VKGQAQPEA IRRHLQEVV RRRDKSGNL TFPRLRHCL VTRCGTYAW ERGKFDLDQ NITVAPLSY KGRAVTEYN IQDYVSEIV SKYLPQGIP PWQIVIIPS SEDQHYILL KLHHVLLNE GLNIGDLLP LIPPTKQGV FVTKSPLVE VLPKLEAIP KLKQRLGEE ISNSWNEFI SNYDPLECT ELLKTTPGF FQLQAITLV ALVSTVKEC RKGFRVIRN DSFSRCKFI FLTFKRECK KRKVTFTNV LKSMLVTLG PRNLLKLFV KCAVMLATW PLRFYLRFC REVMALYNC IVVGYCTYN NTIVGTLYT YVPLVYLCL REVFQYVGI VVKAPRTII EDILLQKES FQTITLCGR KSVAWSDSV KLELLRSIS KSTGVSEIE ILLAAISSC ISKYFIQTK HLIPDSLPI SLRNINSNY IFGTGPNLR PEDSVSGIL CLNLPILDP EKEISTLEN LLEVKTNFR TSLDKQGLS HLLSLCQTK FGFLTKILP LSLLSVYLR YLSRKYAVS VTELTSRYP NVSQKTLWG QEVTSVIYW RPPQANSSV SLCLNEYAD HVRLGVMCD SQLAPHHPL LARSFSEHV HELARAAGV MPTQ
- >TC006336 RA
atgatcgag ttcttggcc actttcgga ctgttttac ttaacggtg gggctgctg cccttgctt gtgtttagc tattttgtt ttaagtctc agccacaaa ctttggatt ttcacaatt tcgattctt caccccgat gttgagttt ttgcgcact ataacagtg cgtaccctc cttgacacg caccgaaat caggggata atttcagta ttgctgtgt gtcaaaggc caggcgcag cctgaggcc attcggagg catctccag gaggtggtg cgaaggcgc gacaaatcg ggaaattta acgtttccg cggctacgc cactgtctg gtcaccagg tgcggaacg tacgcttgg gagcggggc aaattcgat ttggaccag aacattaca gtggcccct ttgagttac aaaggacgg gcagttact gagtacaat atccaggat tatgtgagc gaaattgta tcaaagtat ttgccacag gggattccc ccctggcaa atcgttata atcccctcg tccgaggac caacattac attctgttg aaattgcat catgttttg ttgaacgag gggctgaat attggcgat ttgttaccg ctgattccc cccaccaaa caaggggtt tttgtgacc aaaagcccc ctagttgag gtgttgccc aaactggag gcaatcccc aaactgaag caacgtctc ggggaggaa atctcaaac tcgtggaac gaatttatt tcgaattat gacccgctc gagtgtacc gagttgctt aaaacaaca ccaggtttt tttcaattg caggcaatt acgttggta gcactggtc tcgacagtt aaggagtgt cgtaaaggt tttcgagtt atccgaaat gattccttc tctcggtgt aaatttata tttttgacg ttcaaacgc gaatgcaag aaacgtaaa gtgacattt acaaatgtc ctaaaatcg atgcttgtg actctagga ccgagaaat ctgctgaaa ttgtttgta aaatgtgct gtcatgttg gcaacatgg ccgctgcgg ttttatctc cgtttctgt cgcgaagtc atggctctg tataactgt attgttgtg ggttattgt acttacaac aataccatt gttggtact ttgtatact tatgtacca ttggtctac ctttgcttg agggaggtg ttccaatac gttggtatt gtggttaag gcgccgcgt accataatt gaggatatc ctgctccag aaggaatcc ttccaaact ataactctt tgtgggcga aaatccgtc gcatggtcc gactctgtt aagctcgaa cttttgaga tccatctca aaatctaca ggagtctcc gaaattgag atactccta gctgccatc tcctcctgt atctcaaaa tatttcatc caaaccaag cacttgatc cccgattct ctcccaatc tccctcagg aacatcaat tcgaactac attttcggg accggaccg aacttaagg cctgaggat tcggtcagc ggcatttta tgcctcaat ttaccaatc ctcgacccc gagaaagaa atctcgact ttggagaac ctcctcgaa gtaaaaacc aacttccgg acgagttta gacaaacaa ggcctatca catttgctg agcttgtgc caaacaaaa ttcggattt ttgacaaaa atcctaccc ttatccctc ctctcagtc tatttgcgt tacttatcc cgaaaatac gccgtttct gtgaccgaa ctaaccagc cgctacccc aacgtgagt cagaaaacc ctctggggg caagaagtc accagtgtg atctactgg cgcccccca caggccaac tccagcgtg tcgctctgt cttaacgaa tacgccgat catgtccga ttgggcgta atgtgtgat tcacagctc gccccccac cacccgcta ctggcacga agcttctcc gaacacgtg cacgaactc gcacgagcg gccggcgtc atgccaaca caataa
- >TC006336 RA
cgagtatca attgatttg ttggtaatt gatgtgtac atttgtgcg aagtttgta aaaaatgat cgagttctt ggccacttt cggactgtt ttacttaac ggtggggct gctgccctt gcttgtgtt tagctattt tgttttaag tctcagcca caaactttg gattttcac aatttcgat tcttcaccc cgatgttga gtttttgcg cactataac agtgcgtac cctccttga cacgcaccg aaatcaggg gataatttc agtattgct gtgtgtcaa aggccaggc gcagcctga ggccattcg gaggcatct ccaggaggt ggtgcgaag gcgcgacaa atcgggaaa tttaacgtt tccgcggct acgccactg tctggtcac caggtgcgg aacgtacgc ttgggagcg gggcaaatt cgatttgga ccagaacat tacagtggc ccctttgag ttacaaagg acgggcagt tactgagta caatatcca ggattatgt gagcgaaat tgtatcaaa gtatttgcc acaggggat tcccccctg gcaaatcgt tataatccc ctcgtccga ggaccaaca ttacattct gttgaaatt gcatcatgt tttgttgaa cgaggggct gaatattgg cgatttgtt accgctgat tccccccac caaacaagg ggtttttgt gaccaaaag ccccctagt tgaggtgtt gcccaaact ggaggcaat ccccaaact gaagcaacg tctcgggga ggaaatctc aaactcgtg gaacgaatt tatttcgaa ttatgaccc gctcgagtg taccgagtt gcttaaaac aacaccagg tttttttca attgcaggc aattacgtt ggtagcact ggtctcgac agttaagga gtgtcgtaa aggttttcg agttatccg aaatgattc cttctctcg gtgtaaatt tatattttt gacgttcaa acgcgaatg caagaaacg taaagtgac atttacaaa tgtcctaaa atcgatgct tgtgactct aggaccgag aaatctgct gaaattgtt tgtaaaatg tgctgtcat gttggcaac atggccgct gcggtttta tctccgttt ctgtcgcga agtcatggc tctgtataa ctgtattgt tgtgggtta ttgtactta caacaatac cattgttgg tactttgta tacttatgt accattggt ctacctttg cttgaggga ggtgttcca atacgttgg tattgtggt taaggcgcc gcgtaccat aattgagga tatcctgct ccagaagga atccttcca aactataac tctttgtgg gcgaaaatc cgtcgcatg gtccgactc tgttaagct cgaactttt gagatccat ctcaaaatc tacaggagt ctccgaaat tgagatact cctagctgc catctcctc ctgtatctc aaaatattt catccaaac caagcactt gatccccga ttctctccc aatctccct caggaacat caattcgaa ctacatttt cgggaccgg accgaactt aaggcctga ggattcggt cagcggcat tttatgcct caatttacc aatcctcga ccccgagaa agaaatctc gactttgga gaacctcct cgaagtaaa aaccaactt ccggacgag tttagacaa acaaggcct atcacattt gctgagctt gtgccaaac aaaattcgg atttttgac aaaaatcct acccttatc cctcctctc agtctattt gcgttactt atcccgaaa atacgccgt ttctgtgac cgaactaac cagccgcta ccccaacgt gagtcagaa aaccctctg ggggcaaga agtcaccag tgtgatcta ctggcgccc cccacaggc caactccag cgtgtcgct ctgtcttaa cgaatacgc cgatcatgt ccgattggg cgtaatgtg tgattcaca gctcgcccc ccaccaccc gctactggc acgaagctt ctccgaaca cgtgcacga actcgcacg agcggccgg cgtcatgcc aacacaata aaaaatcaa caaaaatcg tgttttatt caagtgtga ctccccatt cctcacagg gttgccagg tgtagggat ttttgggtg tttttgtgt tcaaagtca caaattaga gaacatttt aataattca catattttt aacaaaaca attaatctt tctagcgat ggacagttg agcaaactt atcacagaa agcgaatta agaagatta cacattact gtaatattt cggaaagga gttaccgaa atttaaata taagtaaat cccagag
