Status
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Gene information
General information
TC006336 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7370899..7376218
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006336
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02898

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006336 RA
      MIEFLATFG LFYLTVGLL PLLVFSYFV LSLSHKLWI 
      FTISILHPD VEFLRTITV RTLLDTHRN QGIISVLLC 
      VKGQAQPEA IRRHLQEVV RRRDKSGNL TFPRLRHCL 
      VTRCGTYAW ERGKFDLDQ NITVAPLSY KGRAVTEYN 
      IQDYVSEIV SKYLPQGIP PWQIVIIPS SEDQHYILL 
      KLHHVLLNE GLNIGDLLP LIPPTKQGV FVTKSPLVE 
      VLPKLEAIP KLKQRLGEE ISNSWNEFI SNYDPLECT 
      ELLKTTPGF FQLQAITLV ALVSTVKEC RKGFRVIRN 
      DSFSRCKFI FLTFKRECK KRKVTFTNV LKSMLVTLG 
      PRNLLKLFV KCAVMLATW PLRFYLRFC REVMALYNC 
      IVVGYCTYN NTIVGTLYT YVPLVYLCL REVFQYVGI 
      VVKAPRTII EDILLQKES FQTITLCGR KSVAWSDSV 
      KLELLRSIS KSTGVSEIE ILLAAISSC ISKYFIQTK 
      HLIPDSLPI SLRNINSNY IFGTGPNLR PEDSVSGIL 
      CLNLPILDP EKEISTLEN LLEVKTNFR TSLDKQGLS 
      HLLSLCQTK FGFLTKILP LSLLSVYLR YLSRKYAVS 
      VTELTSRYP NVSQKTLWG QEVTSVIYW RPPQANSSV 
      SLCLNEYAD HVRLGVMCD SQLAPHHPL LARSFSEHV 
      HELARAAGV MPTQ
    CDS sequence
    • >TC006336 RA
      atgatcgag ttcttggcc actttcgga ctgttttac 
      ttaacggtg gggctgctg cccttgctt gtgtttagc 
      tattttgtt ttaagtctc agccacaaa ctttggatt 
      ttcacaatt tcgattctt caccccgat gttgagttt 
      ttgcgcact ataacagtg cgtaccctc cttgacacg 
      caccgaaat caggggata atttcagta ttgctgtgt 
      gtcaaaggc caggcgcag cctgaggcc attcggagg 
      catctccag gaggtggtg cgaaggcgc gacaaatcg 
      ggaaattta acgtttccg cggctacgc cactgtctg 
      gtcaccagg tgcggaacg tacgcttgg gagcggggc 
      aaattcgat ttggaccag aacattaca gtggcccct 
      ttgagttac aaaggacgg gcagttact gagtacaat 
      atccaggat tatgtgagc gaaattgta tcaaagtat 
      ttgccacag gggattccc ccctggcaa atcgttata 
      atcccctcg tccgaggac caacattac attctgttg 
      aaattgcat catgttttg ttgaacgag gggctgaat 
      attggcgat ttgttaccg ctgattccc cccaccaaa 
      caaggggtt tttgtgacc aaaagcccc ctagttgag 
      gtgttgccc aaactggag gcaatcccc aaactgaag 
      caacgtctc ggggaggaa atctcaaac tcgtggaac 
      gaatttatt tcgaattat gacccgctc gagtgtacc 
      gagttgctt aaaacaaca ccaggtttt tttcaattg 
      caggcaatt acgttggta gcactggtc tcgacagtt 
      aaggagtgt cgtaaaggt tttcgagtt atccgaaat 
      gattccttc tctcggtgt aaatttata tttttgacg 
      ttcaaacgc gaatgcaag aaacgtaaa gtgacattt 
      acaaatgtc ctaaaatcg atgcttgtg actctagga 
      ccgagaaat ctgctgaaa ttgtttgta aaatgtgct 
      gtcatgttg gcaacatgg ccgctgcgg ttttatctc 
      cgtttctgt cgcgaagtc atggctctg tataactgt 
      attgttgtg ggttattgt acttacaac aataccatt 
      gttggtact ttgtatact tatgtacca ttggtctac 
      ctttgcttg agggaggtg ttccaatac gttggtatt 
      gtggttaag gcgccgcgt accataatt gaggatatc 
      ctgctccag aaggaatcc ttccaaact ataactctt 
      tgtgggcga aaatccgtc gcatggtcc gactctgtt 
      aagctcgaa cttttgaga tccatctca aaatctaca 
      ggagtctcc gaaattgag atactccta gctgccatc 
      tcctcctgt atctcaaaa tatttcatc caaaccaag 
      cacttgatc cccgattct ctcccaatc tccctcagg 
      aacatcaat tcgaactac attttcggg accggaccg 
      aacttaagg cctgaggat tcggtcagc ggcatttta 
      tgcctcaat ttaccaatc ctcgacccc gagaaagaa 
      atctcgact ttggagaac ctcctcgaa gtaaaaacc 
      aacttccgg acgagttta gacaaacaa ggcctatca 
      catttgctg agcttgtgc caaacaaaa ttcggattt 
      ttgacaaaa atcctaccc ttatccctc ctctcagtc 
      tatttgcgt tacttatcc cgaaaatac gccgtttct 
      gtgaccgaa ctaaccagc cgctacccc aacgtgagt 
      cagaaaacc ctctggggg caagaagtc accagtgtg 
      atctactgg cgcccccca caggccaac tccagcgtg 
      tcgctctgt cttaacgaa tacgccgat catgtccga 
      ttgggcgta atgtgtgat tcacagctc gccccccac 
      cacccgcta ctggcacga agcttctcc gaacacgtg 
      cacgaactc gcacgagcg gccggcgtc atgccaaca 
      caataa
    mRNA sequence
    • >TC006336 RA
      cgagtatca attgatttg ttggtaatt gatgtgtac 
      atttgtgcg aagtttgta aaaaatgat cgagttctt 
      ggccacttt cggactgtt ttacttaac ggtggggct 
      gctgccctt gcttgtgtt tagctattt tgttttaag 
      tctcagcca caaactttg gattttcac aatttcgat 
      tcttcaccc cgatgttga gtttttgcg cactataac 
      agtgcgtac cctccttga cacgcaccg aaatcaggg 
      gataatttc agtattgct gtgtgtcaa aggccaggc 
      gcagcctga ggccattcg gaggcatct ccaggaggt 
      ggtgcgaag gcgcgacaa atcgggaaa tttaacgtt 
      tccgcggct acgccactg tctggtcac caggtgcgg 
      aacgtacgc ttgggagcg gggcaaatt cgatttgga 
      ccagaacat tacagtggc ccctttgag ttacaaagg 
      acgggcagt tactgagta caatatcca ggattatgt 
      gagcgaaat tgtatcaaa gtatttgcc acaggggat 
      tcccccctg gcaaatcgt tataatccc ctcgtccga 
      ggaccaaca ttacattct gttgaaatt gcatcatgt 
      tttgttgaa cgaggggct gaatattgg cgatttgtt 
      accgctgat tccccccac caaacaagg ggtttttgt 
      gaccaaaag ccccctagt tgaggtgtt gcccaaact 
      ggaggcaat ccccaaact gaagcaacg tctcgggga 
      ggaaatctc aaactcgtg gaacgaatt tatttcgaa 
      ttatgaccc gctcgagtg taccgagtt gcttaaaac 
      aacaccagg tttttttca attgcaggc aattacgtt 
      ggtagcact ggtctcgac agttaagga gtgtcgtaa 
      aggttttcg agttatccg aaatgattc cttctctcg 
      gtgtaaatt tatattttt gacgttcaa acgcgaatg 
      caagaaacg taaagtgac atttacaaa tgtcctaaa 
      atcgatgct tgtgactct aggaccgag aaatctgct 
      gaaattgtt tgtaaaatg tgctgtcat gttggcaac 
      atggccgct gcggtttta tctccgttt ctgtcgcga 
      agtcatggc tctgtataa ctgtattgt tgtgggtta 
      ttgtactta caacaatac cattgttgg tactttgta 
      tacttatgt accattggt ctacctttg cttgaggga 
      ggtgttcca atacgttgg tattgtggt taaggcgcc 
      gcgtaccat aattgagga tatcctgct ccagaagga 
      atccttcca aactataac tctttgtgg gcgaaaatc 
      cgtcgcatg gtccgactc tgttaagct cgaactttt 
      gagatccat ctcaaaatc tacaggagt ctccgaaat 
      tgagatact cctagctgc catctcctc ctgtatctc 
      aaaatattt catccaaac caagcactt gatccccga 
      ttctctccc aatctccct caggaacat caattcgaa 
      ctacatttt cgggaccgg accgaactt aaggcctga 
      ggattcggt cagcggcat tttatgcct caatttacc 
      aatcctcga ccccgagaa agaaatctc gactttgga 
      gaacctcct cgaagtaaa aaccaactt ccggacgag 
      tttagacaa acaaggcct atcacattt gctgagctt 
      gtgccaaac aaaattcgg atttttgac aaaaatcct 
      acccttatc cctcctctc agtctattt gcgttactt 
      atcccgaaa atacgccgt ttctgtgac cgaactaac 
      cagccgcta ccccaacgt gagtcagaa aaccctctg 
      ggggcaaga agtcaccag tgtgatcta ctggcgccc 
      cccacaggc caactccag cgtgtcgct ctgtcttaa 
      cgaatacgc cgatcatgt ccgattggg cgtaatgtg 
      tgattcaca gctcgcccc ccaccaccc gctactggc 
      acgaagctt ctccgaaca cgtgcacga actcgcacg 
      agcggccgg cgtcatgcc aacacaata aaaaatcaa 
      caaaaatcg tgttttatt caagtgtga ctccccatt 
      cctcacagg gttgccagg tgtagggat ttttgggtg 
      tttttgtgt tcaaagtca caaattaga gaacatttt 
      aataattca catattttt aacaaaaca attaatctt 
      tctagcgat ggacagttg agcaaactt atcacagaa 
      agcgaatta agaagatta cacattact gtaatattt 
      cggaaagga gttaccgaa atttaaata taagtaaat 
      cccagag
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