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Gene information
General information
TC030608 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 4843930..4861964
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030608
    iBeetle screen iB_02896
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030608 RA
      MALLNFSAA PFAATPSPP LAPGYYQPP VPYSYPVPQ 
      KPFLREDFG PPQGGHPPY TKVTDDGTK TKVHAVIDY 
      DYDDDEDEG LPPVTPIQG PIFIKNGSV PVVPLYSHP 
      VLNNGTFVQ IPILWTALS VALGLELRG EYIRGVPCI 
      KRHQQLFCP TAGNSYPLD RIELFIDEN KALMKRMYG 
      EFDMGSPGG EYGPGRKRK RSTKPGVPD LHFDPGPDP 
      GGKPGGDSF FSQIRNTRQ NFRNNQSSE SGRIDACES 
      KVEIVTPYW ASNSAGKIR AIVNTQHFE QAIHQEVCA 
      KVRTNRCSG DCGCEQKYK WHRLLAYDP DNDCKGIFM 
      DWFLFPSCC VCRCNPT
    • >TC030608 RB
      MPKRCTYLP TQLILWTAL SVALGLELR GEYIRGVPC 
      IKRHQQLFC PTAGNSYPL DRIELFIDE NKALMKRMY 
      GEFDMGSPG GEYGPGRKR KRSTKPGVP DLHFDPGPD 
      PGGKPGGDS FFSQIRNTR QNFRNNQSS ESGRIDACE 
      SKVEIVTPY WASNSAGKI RAIVNTQHF EQAIHQEVC 
      AKVRTNRCS GDCGCEQKY KWHRLLAYD PDNDCKGIF 
      MDWFLFPSC CVCRCNPT
    CDS sequence
    • >TC030608 RA
      atggccctt ttgaatttc tcagcggcg cccttcgcg 
      gcgacgccc agcccccct ctagccccc ggttactac 
      cagccgccg gtgccctac tcgtacccc gtgccccag 
      aagcccttc ctgcgggag gacttcggg cccccgcaa 
      gggggccac ccgccctat accaaagtt accgatgat 
      ggtactaag actaaagtg cacgcggtt atcgactat 
      gattacgac gacgatgag gacgaagga ctgcccccg 
      gtcaccccc attcagggg ccgattttt atcaaaaat 
      ggttctgtt cctgtcgtg ccgctttac tcgcatccg 
      gtgctcaac aacggcact tttgtgcag attcccata 
      ttatggacg gcgttatcc gtcgcctta ggtttagaa 
      ttacgcggc gagtacatt cgcggcgtc ccttgcata 
      aaacgtcac cagcaattg ttctgccca acggcagga 
      aattcctat ccattagat cgtatagag ttattcata 
      gacgagaac aaggcactg atgaagagg atgtacgga 
      gagttcgac atgggaagt cccggcggc gaatacgga 
      cctggccgg aaaaggaaa agatcaaca aaaccgggc 
      gtcccggac ctgcacttc gatcccgga ccggatccg 
      ggaggaaaa cccggcgga gactcattt tttagtcaa 
      atcagaaac acgagacag aattttcgc aacaaccag 
      agctcagaa agcggccga atcgacgcc tgcgagagc 
      aaagtcgaa attgtgacg ccctattgg gcctcgaat 
      tccgccgga aaaatccgg gccatcgtc aacacgcag 
      catttcgag caagcgata catcaagaa gtctgcgct 
      aaagtgagg acgaaccgt tgttcgggc gattgcgga 
      tgcgagcag aagtacaag tggcaccgc ctactggcc 
      tacgacccg gataacgac tgcaagggc atttttatg 
      gattggttc ctatttccg tcgtgttgt gtctgccga 
      tgcaacccg acttaa
    • >TC030608 RB
      atgccaaaa cgatgcact tacctcccg acccaactg 
      atattatgg acggcgtta tccgtcgcc ttaggttta 
      gaattacgc ggcgagtac attcgcggc gtcccttgc 
      ataaaacgt caccagcaa ttgttctgc ccaacggca 
      ggaaattcc tatccatta gatcgtata gagttattc 
      atagacgag aacaaggca ctgatgaag aggatgtac 
      ggagagttc gacatggga agtcccggc ggcgaatac 
      ggacctggc cggaaaagg aaaagatca acaaaaccg 
      ggcgtcccg gacctgcac ttcgatccc ggaccggat 
      ccgggagga aaacccggc ggagactca ttttttagt 
      caaatcaga aacacgaga cagaatttt cgcaacaac 
      cagagctca gaaagcggc cgaatcgac gcctgcgag 
      agcaaagtc gaaattgtg acgccctat tgggcctcg 
      aattccgcc ggaaaaatc cgggccatc gtcaacacg 
      cagcatttc gagcaagcg atacatcaa gaagtctgc 
      gctaaagtg aggacgaac cgttgttcg ggcgattgc 
      ggatgcgag cagaagtac aagtggcac cgcctactg 
      gcctacgac ccggataac gactgcaag ggcattttt 
      atggattgg ttcctattt ccgtcgtgt tgtgtctgc 
      cgatgcaac ccgacttaa
    mRNA sequence
    • >TC030608 RA
      ccgaccaaa ctctccgac taatgtcac ccagtcgcg 
      atcgcggtg tgccgagag ataagcttt cgatggctt 
      cgtcttgtg ggtggacgt gacggcgcc ccgatggcc 
      cttttgaat ttctcagcg gcgcccttc gcggcgacg 
      cccagcccc cctctagcc cccggttac taccagccg 
      ccggtgccc tactcgtac cccgtgccc cagaagccc 
      ttcctgcgg gaggacttc gggcccccg caagggggc 
      cacccgccc tataccaaa gttaccgat gatggtact 
      aagactaaa gtgcacgcg gttatcgac tatgattac 
      gacgacgat gaggacgaa ggactgccc ccggtcacc 
      cccattcag gggccgatt tttatcaaa aatggttct 
      gttcctgtc gtgccgctt tactcgcat ccggtgctc 
      aacaacggc acttttgtg cagattccc atattatgg 
      acggcgtta tccgtcgcc ttaggttta gaattacgc 
      ggcgagtac attcgcggc gtcccttgc ataaaacgt 
      caccagcaa ttgttctgc ccaacggca ggaaattcc 
      tatccatta gatcgtata gagttattc atagacgag 
      aacaaggca ctgatgaag aggatgtac ggagagttc 
      gacatggga agtcccggc ggcgaatac ggacctggc 
      cggaaaagg aaaagatca acaaaaccg ggcgtcccg 
      gacctgcac ttcgatccc ggaccggat ccgggagga 
      aaacccggc ggagactca ttttttagt caaatcaga 
      aacacgaga cagaatttt cgcaacaac cagagctca 
      gaaagcggc cgaatcgac gcctgcgag agcaaagtc 
      gaaattgtg acgccctat tgggcctcg aattccgcc 
      ggaaaaatc cgggccatc gtcaacacg cagcatttc 
      gagcaagcg atacatcaa gaagtctgc gctaaagtg 
      aggacgaac cgttgttcg ggcgattgc ggatgcgag 
      cagaagtac aagtggcac cgcctactg gcctacgac 
      ccggataac gactgcaag ggcattttt atggattgg 
      ttcctattt ccgtcgtgt tgtgtctgc cgatgcaac 
      ccgacttaa gtaatttcg cacacaggt tttcgggga 
      gtagacagg ggcggggct aggccaagc cccgcccgc 
      atggtctac ttaggaaaa ttaatactc aaagtgcca 
      aattgatca catcactaa tccctccca taaaattga 
      tacaaagaa aatgtgcaa tcaattcgt gactgatat 
      tttgtaatc tacgttacc aatcagtcc tgttcttgg 
      ttacgaatc tagttacca ctttagaag tatttttta 
      cgatttatt agggctatt tataaaaag taattgatt 
      aatgtcgta agttgtagc agatggctg ccattttct 
      gtagcatta ttttgtaat aaattattt gtaaaata
    • >TC030608 RB
      acatgttgg aaacacatc accgtcgcg aaaaaatgt 
      gctcgtgtc gtttgattt agtgacaat caattcgaa 
      agcgcaaca atgccaaaa cgatgcact tacctcccg 
      acccaactg atattatgg acggcgtta tccgtcgcc 
      ttaggttta gaattacgc ggcgagtac attcgcggc 
      gtcccttgc ataaaacgt caccagcaa ttgttctgc 
      ccaacggca ggaaattcc tatccatta gatcgtata 
      gagttattc atagacgag aacaaggca ctgatgaag 
      aggatgtac ggagagttc gacatggga agtcccggc 
      ggcgaatac ggacctggc cggaaaagg aaaagatca 
      acaaaaccg ggcgtcccg gacctgcac ttcgatccc 
      ggaccggat ccgggagga aaacccggc ggagactca 
      ttttttagt caaatcaga aacacgaga cagaatttt 
      cgcaacaac cagagctca gaaagcggc cgaatcgac 
      gcctgcgag agcaaagtc gaaattgtg acgccctat 
      tgggcctcg aattccgcc ggaaaaatc cgggccatc 
      gtcaacacg cagcatttc gagcaagcg atacatcaa 
      gaagtctgc gctaaagtg aggacgaac cgttgttcg 
      ggcgattgc ggatgcgag cagaagtac aagtggcac 
      cgcctactg gcctacgac ccggataac gactgcaag 
      ggcattttt atggattgg ttcctattt ccgtcgtgt 
      tgtgtctgc cgatgcaac ccgacttaa gtaatttcg 
      cacacaggt tttcgggga gtagacagg ggcggggct 
      aggccaagc cccgcccgc atggtctac ttaggaaaa 
      ttaatactc aaagtgcca aattgatca catcactaa 
      tccctccca taaaattga tacaaagaa aatgtgcaa 
      tcaattcgt gactgatat tttgtaatc tacgttacc 
      aatcagtcc tgttcttgg ttacgaatc tagttacca 
      ctttagaag tatttttta cgatttatt agggctatt 
      tataaaaag taattgatt aatgtcgta agttgtagc 
      agatggctg ccattttct gtagcatta ttttgtaat 
      aaattattt gtaaaata