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Gene information
General information
TC006213 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5114198..5124650
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006213
    iBeetle screen iB_02895

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006213 RA
      MNRVTNGNL AMPPSVVDK SKDWQLEIL MEKLRSKAS 
      QYKSLTEIS KNVRMAMLD KRYALDSVE KSQHQKCLD 
      TLQHSIKVT SLQSMIERL ESLTRQLGL KFMVGPSGV 
      ELFISSDMF YLEIVLDQT GAVKDVKVH HDSKLEQQS 
      CAELVNCLS SGDFADFTA QLEGFASIY QLNAEKKVK 
      CKAFTALQS LESDLSTLA HLQSFMKEP FNLLYKSPV 
      GVLEKRRGG HPMKLTYFV SPYDLLNVE KCDIEPITV 
      DTIVSKALG YSVTVCMEG SATHKLQTT TLITVNRNI 
      NGKSTPSFS SLSSQNSAA IPACFVLKL NKPMPMCVN 
      LVRQIQQVT ELDTIEQSA THPLLSLIV HHASEGKME 
      SGNNKGLFV TLPDQTHCY FMTENKNMD GVLVSSIPF 
      THPAHVSQI LVILRQQAL FNTIIGSCV RPNSKQDFE 
      NMTMFEISA LSWTHISLS LEHPIEESM ATAEIDLSD 
      ISNLVCKIV SPGTPPPPN APDTASDLS TKILNRCFS 
      IPVTMRAVL KLWEKQTTK RHYNGHENF SLPLGSGDP 
      GGHKGAPGG NLTEFGGLD KIKQEPLGN GSHGMMMQS 
      HQGMFLNES MIASANFPN FQTSENMLT SMELTNILA 
      GANQSDKMK RPHKRKSDD LWKNKRKLG EGADPDLLV 
      ETSSSDSTS RSTPISQEN EIATPNSGL GFQSDLELM 
      SGLDPADLL AESDKVGGD FGALDELGD VEEMLAGPS 
      RRKSHDQKS PTIMMDLGE KSLVPPSVS ITPIASTSP 
      GFNQGGNAE KRPGIEIIA LATSAATAL PSSITITPI 
      TTSQSKGDE KNREKKRSK SSSDDKGRL EKKRKRKRD 
      ESPMGPPDK VPPKQDPLT KPVTVSIKP AESPPLNMS 
      TPTSPSMMR KFNASPTQN RNISMSGKL SPNLMKPTL 
      KTGSSHHSP KHSPAHVPS SPKHIIPGI SSPKSHGTS 
      PKHPSTSGS GKPSMSTLK SAANSPKNS DSKTKSTSK 
      DSSRDKEKK ISGVFNVAS KNKSSVKVK PLDLNTENL 
      SGVQDGLPS PSSTDLSKS ATSNQVRNR KGSLSAIVD 
      KLKSAQHCD GVTDLSSKG ASSGGNKDR SGQVSTKLN 
      EGKSGKIGE TKNSEYMVK PSSDGMKIT INKTRSKES 
      KTSVSKSGS GSPKTHTGL KPGVNSGPA SKKPQHLGQ 
      KLVNSTSGP NYSSLKSKA PSGAKLSSG PSKLTKLSN 
      SPKSATDLS RKDRPKLNK SSSEKSIFT SKDGRKSSP 
      TSNRDESDG IYKMSKMDH YNPPSIVEG LMTVRQLDK 
      NFQIPKLSA RASVDDKKP KVSSDNLNN INRTVDTKI 
      FDMMNKSDL KYPLALPQK TFDNAVDVK LRNNVTPLP 
      SSSPKLKDE DDKKDQPQN LAQTKDDSA YKVNYPIAV 
      SKSVLPPIS EPLNLYTKS IDLTSKFVA PAPKDDKKD 
      VKKNDDVLL DFSAAGKID KGAAVVTFP QSPSVSVHI 
      VKSPAPSPL IAPSPHSAS PCITDDELM DEAVIGIGK
    CDS sequence
    • >TC006213 RA
      atgaatcgc gtaacaaac ggcaattta gcgatgcca 
      ccgtccgtt gtggacaaa tccaaagac tggcagttg 
      gagatattg atggagaag ctgcgctcg aaggcgtcg 
      cagtacaaa agcttgaca gagatatcc aaaaatgtg 
      cgaatggcc atgcttgac aagcgctac gcgttggac 
      agcgtcgag aagagccag caccagaaa tgcctcgac 
      actctgcag cactccatc aaagtgacg tccttgcag 
      agcatgatc gagcggctg gagagcttg acgcggcag 
      ctggggctc aagttcatg gtgggcccc tcgggggtg 
      gagctcttc atctcctcc gacatgttc tacctggag 
      atagtgctc gaccagacg ggggccgtc aaagacgtg 
      aaagtgcac catgacagt aaattggag caacagagt 
      tgcgctgag ctcgtcaat tgtttatct agtggcgat 
      tttgccgat ttcactgcc cagttggaa ggttttgcc 
      tcgatttac cagctcaat gctgagaaa aaggtcaag 
      tgtaaggcg ttcacagcc ttgcagtcg ctggagtct 
      gatttgagc accttggct cacttgcag tctttcatg 
      aaggaacct ttcaacctg ctgtataag tctccggtt 
      ggggtgctg gagaagcgc cgaggggga caccccatg 
      aaactcacc tattttgtc tctccttat gatttatta 
      aatgttgag aaatgtgat attgaacct attactgtt 
      gatacgatc gttagtaaa gcgctgggg tacagtgtg 
      acggtgtgt atggaaggg tccgctaca cataagttg 
      caaacgacc actctcatc acagttaat aggaatatt 
      aacgggaaa agtacgccg tctttttca tcgctaagt 
      agccaaaat agtgcagca attccggcc tgttttgtc 
      ctcaaatta aacaaacca atgccaatg tgtgtcaat 
      ttagttaga caaattcaa caagttact gaattggat 
      actatagaa caatcagca actcatccg ttacttagt 
      ttaatagta catcacgcc agtgaaggg aaaatggag 
      tctgggaac aataagggc ctttttgtg actttgccc 
      gaccaaaca cattgttat ttcatgact gaaaacaaa 
      aatatggac ggcgtttta gtttcaagc attccattc 
      acccaccca gcacatgtg tcccaaatt ttagtaatc 
      ttaagacaa caagctctc ttcaacacg ataatcggc 
      agttgtgtg cgtcctaat agcaaacaa gattttgaa 
      aatatgacc atgtttgag ataagtgcc ctttcctgg 
      acccacatc tccctctcc ctcgagcac ccaattgaa 
      gaaagcatg gcaacggcc gaaatcgac ctcagcgac 
      atctccaac cttgtttgt aaaatcgta agtcccggt 
      acaccgccg ccgcccaac gcccccgac accgcctcc 
      gatctaagc acgaaaatt ttaaaccgg tgtttttcc 
      attccggtg acgatgcga gccgtcttg aaactatgg 
      gagaaacaa accactaaa cgccactac aacggacat 
      gaaaatttt agtttaccc ctcggttcc ggcgaccct 
      gggggtcac aagggggcc ccaggcggc aatttaacg 
      gaattcggg gggttagac aagatcaag caggagccc 
      cttggcaac ggcagccac ggcatgatg atgcaaagc 
      catcaaggc atgtttttg aacgaatct atgatcgct 
      agtgccaat tttcccaat tttcaaacc agtgaaaat 
      atgctcact agcatggag ctgactaat atactggca 
      ggggccaac caatcggat aaaatgaaa cggccccac 
      aagcgcaaa tcggacgat ttgtggaaa aacaagcga 
      aagttgggc gagggggct gaccctgac cttctggtc 
      gagacgtca agtagcgac tccacgtcg cgaagtacc 
      ccaatctcg caggagaat gaaattgcg accccaaat 
      tcggggttg gggtttcag tcggatctg gagcttatg 
      agtggctta gatccggcg gatttgttg gccgagtcg 
      gataaagtc ggcggtgat tttggggcc ttagatgag 
      ttgggggat gtggaggag atgttggcg gggccgtcg 
      aggcggaaa tcgcacgat cagaaatca ccaactatt 
      atgatggat ttgggggag aaaagtctg gtaccacca 
      agtgttagt attacgccg attgcgagt acttcaccg 
      gggtttaat caggggggt aatgcggaa aaaaggccc 
      gggattgag attatagca cttgcgacg tcagctgcg 
      actgcattg cccagttct attacgatt acgccgatt 
      acgacgtcg caaagtaaa ggggatgag aagaatcgg 
      gagaagaag aggagcaaa agtagttcg gatgataag 
      gggcgtctt gagaagaag cgtaagcga aaacgcgac 
      gagagcccg atgggcccc ccggacaag gtgcccccg 
      aagcaggac cccctcacc aaaccggtc accgtcagc 
      ataaaaccg gccgagtct ccgcccctc aacatgagc 
      accccaaca agccccagc atgatgcga aaattcaac 
      gcttcgccc actcaaaat cgcaatatt tccatgagt 
      ggtaaacta agccccaac ctaatgaaa cccactctc 
      aaaacgggc tcatcgcat cacagtcct aaacactcc 
      ccagcccat gttcccagt agtcctaaa cacataatt 
      cccggtatt tcgagtcct aaaagccac ggtacttcc 
      cccaaacac ccttcgacg agtggttcc ggtaaaccg 
      agcatgtcc acattgaaa agtgccgcg aactcaccg 
      aaaaattcc gattctaag acgaaatcc acctcgaag 
      gattcgtcc agagacaag gagaaaaaa atttcgggt 
      gtgtttaac gttgcgtcg aaaaataaa tcatcagtg 
      aaagtgaaa ccgttggac ttgaatact gaaaatttg 
      agcggggtt caggacggg ttaccgtca cccagttcc 
      acggacttg tcaaaatca gcgacttcg aatcaagtg 
      aggaaccga aaaggctca ctgtctgcc attgtcgat 
      aagttaaag tcggcccaa cattgcgat ggagtcacc 
      gatttgagt agcaagggg gctagtagt ggaggaaac 
      aaagacaga agtgggcaa gtttcaacg aaactaaac 
      gagggaaaa agtgggaaa atcggtgaa acgaaaaat 
      tcggaatat atggttaag cccagttcg gacggcatg 
      aaaatcaca ataaataaa accagaagt aaagaaagt 
      aaaacaagt gtgagtaaa tcaggctca gggtcgccc 
      aaaactcac acggggtta aaaccgggg gttaacagc 
      ggccctgcg tcgaaaaaa ccccaacat ttggggcaa 
      aaacttgta aattcgaca agtggcccc aattattca 
      agtttgaag agtaaagcc ccttcgggg gctaaacta 
      tcaagcggg ccttcgaaa ctaacaaaa ctgagtaat 
      tcaccgaaa agtgcgacc gatttgagc cgaaaagac 
      cgcccaaaa ctgaacaaa agcagttca gagaagtcg 
      attttcacg tcgaaggat ggccgaaaa tcgagtccg 
      acttcaaac agggacgag agtgacggt atttacaag 
      atgtcgaaa atggatcat tataaccca ccttcgatt 
      gtcgagggt ttaatgaca gtgcgtcaa ttagacaaa 
      aatttccaa ataccgaaa ctatcggca cgtgcatca 
      gtcgacgac aaaaaaccg aaagtaagt tcggataat 
      ttaaacaac atcaatcgg actgtcgat acgaaaatc 
      ttcgatatg atgaacaag tcggacctc aaatacccc 
      ctcgccctc cctcaaaaa accttcgac aatgcggtc 
      gatgtcaaa ttgaggaac aacgtcacg cccctccct 
      agctctagt ccaaaactg aaagacgaa gacgataaa 
      aaggaccaa ccccagaac ctcgcccag accaaggac 
      gactccgcc tataaagtg aattatcca atcgcggtg 
      tccaaatcg gtgctcccg cccatttcc gaacccctc 
      aacctctac acgaagagc atcgatttg acgtcgaag 
      ttcgtggcg cccgccccc aaagacgat aaaaaggac 
      gtgaagaag aacgacgat gtgctttta gacttttcg 
      gcggccgga aaaatcgat aagggtgca gccgtggtt 
      acgtttccc caatcgcct tcggttagt gttcatata 
      gttaagtca ccggcgccg tcgccgttg atcgcacca 
      agtccacat tcggcctcg ccgtgtatc actgacgat 
      gagttgatg gacgaggcg gtgataggt ataggaaaa 
      tag
    mRNA sequence
    • >TC006213 RA
      agtaacgaa actgtcaag tttttgttt ggtgctgat 
      gtatgttcg aaaaatcgg tttttttcc cttaattcg 
      ccccaaata gaaaatgtt tacgaacat ttaacgtta 
      atttcgccc gaactcatc atgaatcgc gtaacaaac 
      ggcaattta gcgatgcca ccgtccgtt gtggacaaa 
      tccaaagac tggcagttg gagatattg atggagaag 
      ctgcgctcg aaggcgtcg cagtacaaa agcttgaca 
      gagatatcc aaaaatgtg cgaatggcc atgcttgac 
      aagcgctac gcgttggac agcgtcgag aagagccag 
      caccagaaa tgcctcgac actctgcag cactccatc 
      aaagtgacg tccttgcag agcatgatc gagcggctg 
      gagagcttg acgcggcag ctggggctc aagttcatg 
      gtgggcccc tcgggggtg gagctcttc atctcctcc 
      gacatgttc tacctggag atagtgctc gaccagacg 
      ggggccgtc aaagacgtg aaagtgcac catgacagt 
      aaattggag caacagagt tgcgctgag ctcgtcaat 
      tgtttatct agtggcgat tttgccgat ttcactgcc 
      cagttggaa ggttttgcc tcgatttac cagctcaat 
      gctgagaaa aaggtcaag tgtaaggcg ttcacagcc 
      ttgcagtcg ctggagtct gatttgagc accttggct 
      cacttgcag tctttcatg aaggaacct ttcaacctg 
      ctgtataag tctccggtt ggggtgctg gagaagcgc 
      cgaggggga caccccatg aaactcacc tattttgtc 
      tctccttat gatttatta aatgttgag aaatgtgat 
      attgaacct attactgtt gatacgatc gttagtaaa 
      gcgctgggg tacagtgtg acggtgtgt atggaaggg 
      tccgctaca cataagttg caaacgacc actctcatc 
      acagttaat aggaatatt aacgggaaa agtacgccg 
      tctttttca tcgctaagt agccaaaat agtgcagca 
      attccggcc tgttttgtc ctcaaatta aacaaacca 
      atgccaatg tgtgtcaat ttagttaga caaattcaa 
      caagttact gaattggat actatagaa caatcagca 
      actcatccg ttacttagt ttaatagta catcacgcc 
      agtgaaggg aaaatggag tctgggaac aataagggc 
      ctttttgtg actttgccc gaccaaaca cattgttat 
      ttcatgact gaaaacaaa aatatggac ggcgtttta 
      gtttcaagc attccattc acccaccca gcacatgtg 
      tcccaaatt ttagtaatc ttaagacaa caagctctc 
      ttcaacacg ataatcggc agttgtgtg cgtcctaat 
      agcaaacaa gattttgaa aatatgacc atgtttgag 
      ataagtgcc ctttcctgg acccacatc tccctctcc 
      ctcgagcac ccaattgaa gaaagcatg gcaacggcc 
      gaaatcgac ctcagcgac atctccaac cttgtttgt 
      aaaatcgta agtcccggt acaccgccg ccgcccaac 
      gcccccgac accgcctcc gatctaagc acgaaaatt 
      ttaaaccgg tgtttttcc attccggtg acgatgcga 
      gccgtcttg aaactatgg gagaaacaa accactaaa 
      cgccactac aacggacat gaaaatttt agtttaccc 
      ctcggttcc ggcgaccct gggggtcac aagggggcc 
      ccaggcggc aatttaacg gaattcggg gggttagac 
      aagatcaag caggagccc cttggcaac ggcagccac 
      ggcatgatg atgcaaagc catcaaggc atgtttttg 
      aacgaatct atgatcgct agtgccaat tttcccaat 
      tttcaaacc agtgaaaat atgctcact agcatggag 
      ctgactaat atactggca ggggccaac caatcggat 
      aaaatgaaa cggccccac aagcgcaaa tcggacgat 
      ttgtggaaa aacaagcga aagttgggc gagggggct 
      gaccctgac cttctggtc gagacgtca agtagcgac 
      tccacgtcg cgaagtacc ccaatctcg caggagaat 
      gaaattgcg accccaaat tcggggttg gggtttcag 
      tcggatctg gagcttatg agtggctta gatccggcg 
      gatttgttg gccgagtcg gataaagtc ggcggtgat 
      tttggggcc ttagatgag ttgggggat gtggaggag 
      atgttggcg gggccgtcg aggcggaaa tcgcacgat 
      cagaaatca ccaactatt atgatggat ttgggggag 
      aaaagtctg gtaccacca agtgttagt attacgccg 
      attgcgagt acttcaccg gggtttaat caggggggt 
      aatgcggaa aaaaggccc gggattgag attatagca 
      cttgcgacg tcagctgcg actgcattg cccagttct 
      attacgatt acgccgatt acgacgtcg caaagtaaa 
      ggggatgag aagaatcgg gagaagaag aggagcaaa 
      agtagttcg gatgataag gggcgtctt gagaagaag 
      cgtaagcga aaacgcgac gagagcccg atgggcccc 
      ccggacaag gtgcccccg aagcaggac cccctcacc 
      aaaccggtc accgtcagc ataaaaccg gccgagtct 
      ccgcccctc aacatgagc accccaaca agccccagc 
      atgatgcga aaattcaac gcttcgccc actcaaaat 
      cgcaatatt tccatgagt ggtaaacta agccccaac 
      ctaatgaaa cccactctc aaaacgggc tcatcgcat 
      cacagtcct aaacactcc ccagcccat gttcccagt 
      agtcctaaa cacataatt cccggtatt tcgagtcct 
      aaaagccac ggtacttcc cccaaacac ccttcgacg 
      agtggttcc ggtaaaccg agcatgtcc acattgaaa 
      agtgccgcg aactcaccg aaaaattcc gattctaag 
      acgaaatcc acctcgaag gattcgtcc agagacaag 
      gagaaaaaa atttcgggt gtgtttaac gttgcgtcg 
      aaaaataaa tcatcagtg aaagtgaaa ccgttggac 
      ttgaatact gaaaatttg agcggggtt caggacggg 
      ttaccgtca cccagttcc acggacttg tcaaaatca 
      gcgacttcg aatcaagtg aggaaccga aaaggctca 
      ctgtctgcc attgtcgat aagttaaag tcggcccaa 
      cattgcgat ggagtcacc gatttgagt agcaagggg 
      gctagtagt ggaggaaac aaagacaga agtgggcaa 
      gtttcaacg aaactaaac gagggaaaa agtgggaaa 
      atcggtgaa acgaaaaat tcggaatat atggttaag 
      cccagttcg gacggcatg aaaatcaca ataaataaa 
      accagaagt aaagaaagt aaaacaagt gtgagtaaa 
      tcaggctca gggtcgccc aaaactcac acggggtta 
      aaaccgggg gttaacagc ggccctgcg tcgaaaaaa 
      ccccaacat ttggggcaa aaacttgta aattcgaca 
      agtggcccc aattattca agtttgaag agtaaagcc 
      ccttcgggg gctaaacta tcaagcggg ccttcgaaa 
      ctaacaaaa ctgagtaat tcaccgaaa agtgcgacc 
      gatttgagc cgaaaagac cgcccaaaa ctgaacaaa 
      agcagttca gagaagtcg attttcacg tcgaaggat 
      ggccgaaaa tcgagtccg acttcaaac agggacgag 
      agtgacggt atttacaag atgtcgaaa atggatcat 
      tataaccca ccttcgatt gtcgagggt ttaatgaca 
      gtgcgtcaa ttagacaaa aatttccaa ataccgaaa 
      ctatcggca cgtgcatca gtcgacgac aaaaaaccg 
      aaagtaagt tcggataat ttaaacaac atcaatcgg 
      actgtcgat acgaaaatc ttcgatatg atgaacaag 
      tcggacctc aaatacccc ctcgccctc cctcaaaaa 
      accttcgac aatgcggtc gatgtcaaa ttgaggaac 
      aacgtcacg cccctccct agctctagt ccaaaactg 
      aaagacgaa gacgataaa aaggaccaa ccccagaac 
      ctcgcccag accaaggac gactccgcc tataaagtg 
      aattatcca atcgcggtg tccaaatcg gtgctcccg 
      cccatttcc gaacccctc aacctctac acgaagagc 
      atcgatttg acgtcgaag ttcgtggcg cccgccccc 
      aaagacgat aaaaaggac gtgaagaag aacgacgat 
      gtgctttta gacttttcg gcggccgga aaaatcgat 
      aagggtgca gccgtggtt acgtttccc caatcgcct 
      tcggttagt gttcatata gttaagtca ccggcgccg 
      tcgccgttg atcgcacca agtccacat tcggcctcg 
      ccgtgtatc actgacgat gagttgatg gacgaggcg 
      gtgataggt ataggaaaa taggacacg gccggtaag 
      gtagggcac gacctaatc tcaaattcg gtgtaaata 
      gggaggtta ggtctgcga gtttgtgcg cgcgcctcg 
      tatatacag tgcgattgg ttgagtgtg atctgcgtc 
      ttccagttg aatttttaa aacgaaaag agaaaacaa 
      ccgaattaa gcaattcct taaacaatt cagatttgt 
      gttgcctag ggtgaccag attggcctt aacaaaaaa 
      catgtgtac tatattatt caaataaaa gaaatatca 
      aaat
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