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Gene information
General information
TC033987
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006193
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 4509847..4513021
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033987
    iBeetle screen iB_02894
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033987 RA
      MIPQLLFLV LVIFRISVN SVRTCYFCA 
      DFFENCDAN DRSDTPKKM NCDTFKVNR 
      EQSMLEYFR EPNFLTNYD IYHLFGPAL 
      SERNVKKLC IKVVYGYKD HNGIYDDGK 
      NFVSYQELR NLTFQSCMF ERFAYYKNA 
      CDYFQQRMG RKIKNVSLF DFYHCQTCR 
      RHECNGQSR TGFSWDLGL VCLIFVYLV 
      ARD
    CDS sequence
    • >TC033987 RA
      atgatccct caactactt tttttggtt ttggttata 
      tttcgcatt tcagtaaat tcggtgcgc acttgttac 
      ttctgtgcg gactttttc gaaaattgc gatgcaaac 
      gacagatcg gacactccg aaaaaaatg aattgtgac 
      acatttaag gtaaaccgc gagcaatca atgctcgaa 
      tacttccga gagcccaat tttctcacc aattacgac 
      atttaccac ctgttcggt ccagccctt tccgagcgg 
      aacgtcaaa aaactttgc attaaagtg gtttacggc 
      tacaaggac cataatggg atttacgac gatgggaag 
      aatttcgta tcatatcag gaactgaga aatttgacg 
      ttccagtcg tgtatgttc gaaaggttt gcgtactat 
      aaaaatgcc tgtgactac tttcaacaa cggatgggc 
      aggaaaatt aaaaatgta tcgcttttc gacttttac 
      cactgccaa acttgcaga aggcacgaa tgcaacggc 
      cagagtaga acgggattt agctgggat cttggttta 
      gtgtgtctc atttttgtg tacttagta gcgagggac 
      taa
    mRNA sequence
    • >TC033987 RA
      tttatggcc caaatgacg aaaataaat gattcatgt 
      ttactcatt actgattac tgattattc ctataataa 
      aagaagaac tccaaaaaa tagttcaga tatcagctg 
      caaaatgct taaaacagg tgttgtggt acttctgtg 
      ctcagttat ttccttgac tggggcctt gcagtgtta 
      cgaatgctc cgcattaaa aaacatcga ctgctacag 
      ccccatcaa caagacatc aattccaat cacaaattg 
      tactgggat aggctacca ggaaaacac aacgtatga 
      atgtgttgc ctataagga gcatcgtaa ttcaataga 
      cttgggcca aaattcgaa attaaatat tttttttta 
      gataatgag acgggccaa gctttcgtc ggccgtcaa 
      tgtattatg agaagtcaa tatgactat tgtactgcc 
      tataaagaa aaccgtgaa ttatatgga ttcatcgta 
      gatgactgt tttacttgc aaggaagat ttatgcaat 
      tctggaaca aatgtacaa atgatgttt ggctttttt 
      agctttata tttttgctt tatgtaatt caataaata 
      atctggttt gaattttga ttgattaat taatcaaaa 
      atgacacgt gaaacatga tccctcaac tactttttt 
      tggttttgg ttatatttc gcatttcag taaattcgg 
      tgcgcactt gttacttct gtgcggact ttttcgaaa 
      attgcgatg caaacgaca gatcggaca ctccgaaaa 
      aaatgaatt gtgacacat ttaaggtaa accgcgagc 
      aatcaatgc tcgaatact tccgagagc ccaattttc 
      tcaccaatt acgacattt accacctgt tcggtccag 
      ccctttccg agcggaacg tcaaaaaac tttgcatta 
      aagtggttt acggctaca aggaccata atgggattt 
      acgacgatg ggaagaatt tcgtatcat atcaggaac 
      tgagaaatt tgacgttcc agtcgtgta tgttcgaaa 
      ggtttgcgt actataaaa atgcctgtg actactttc 
      aacaacgga tgggcagga aaattaaaa atgtatcgc 
      ttttcgact tttaccact gccaaactt gcagaaggc 
      acgaatgca acggccaga gtagaacgg gatttagct 
      gggatcttg gtttagtgt gtctcattt ttgtgtact 
      tagtagcga gggactaat aaattttta acgatgatg 
      tcatcgctg tacccactt tacaa