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Gene information
General information
TC006162 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 3858841..3862445
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006162
    iBeetle screen iB_02893
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006162 RA
      MSLFGSLMG DIEEDPFFG SHMNMMRQM NTMMNSFFS 
      DPFSMGFGD FDRGHRMSN SLMPFSMPI MPNFNRLLS 
      GSLDSLGAH SYSSSTVVS MSSGPDGRP QVYKATSST 
      RTAPGGIKE TQKTVTDTR SGTKKMAIG HHIGERAHI 
      IEKEQNMHT GDREERQDF INLDEEEAE DFNKEWETK 
      TRSRSEIPR ICSGSVRNR HGYGAPGGM LAITGGPSR 
      RRQRQSTPM VSPRRSIRS SPLTVPQAS ASSRTPYSP 
      PGQVQNVRK SKNVKTVSG SPPPPPPAD L
    • >TC006162 RB
      MSLFGSLMG DIEEDPFFG SHMNMMRQM NTMMNSFFS 
      DPFSMGFGD FDRGHRMSN SLMPFSMPI MPNFNRLLS 
      GSLDSLGAH SYSSSTVVS MSSGPDGRP QVYKATSST 
      RTAPGGIKE TQKTVTDTR SGTKKMAIG HHIGERAHI 
      IEKEQNMHT GDREERQDF INLDEEEAE DFNKEWETK 
      TRSRSEIPR ICSGSVRNR HGYGAPGGM LAITGGPRR 
      RQRQSTPMV SPRRSIRSS PLTVPQASA SSRTPYSPP 
      GQVQNVRKS KNVKTVSGS PPPPPPADL
    • >TC006162 RC
      MSLFGSLMG DIEEDPFFG SHMNMMRQM NTMMNSFFS 
      DPFSMGFGD FDRGHRMSN SLMPFSMPI MPNFNRLLS 
      GSLDSLGAH SYSSSTVVS MSSGPDGRP QVYKATSST 
      RTAPGGIKE TQKTVTDTR SGTKKMAIG HHIGERAHI 
      IEKEQNMHT GDREERQDF INLDEEEAE DFNKEWETK 
      TRSRSEIPR ICSGSVRNR HGYGAPGGM LAITGGPSS 
      RTPYSPPGQ VQNVRKSKN VKTVSGSPP PPPPADL
    CDS sequence
    • >TC006162 RA
      atgtctctc ttcggctca ttaatgggc gatattgag 
      gaggatccg tttttcgga tctcacatg aatatgatg 
      cgccaaatg aacacaatg atgaattcc ttcttctcg 
      gaccctttt tcgatgggt ttcggtgat ttcgatcgc 
      ggtcaccgc atgagcaac tctcttatg cctttttca 
      atgccaatt atgccaaat ttcaaccgg ctgttgagc 
      ggctcgttg gactccctg ggggcgcac agttacagt 
      tccagcacc gtggttagc atgtcttcg gggcccgac 
      ggccgcccc caggtgtac aaagccacg tcgagcacg 
      cgcacagct cctgggggt attaaagag acccaaaag 
      acagtcact gacacacgg tcagggacc aagaaaatg 
      gccatcggg caccacatc ggcgaacgg gcccacatc 
      atagaaaag gagcagaat atgcacact ggggaccgt 
      gaggaaagg caggatttt atcaatttg gacgaggaa 
      gaggccgag gattttaat aaagagtgg gagactaag 
      actaggagt cgttcggag attcccagg atttgttcg 
      gggtctgtg cgaaaccga catgggtat ggggctcca 
      ggaggcatg ctggcgatc actggaggg cctagtaga 
      cgtcggcaa agacagtca accccaatg gtctcgcct 
      cgtagaagt ataagatcg tcgccgtta actgtgcca 
      caggcgtcg gctagctct aggactcct tacagcccc 
      ccgggacaa gtccaaaat gtcaggaag agtaaaaat 
      gttaagacg gtgtcagga tcaccacca ccaccacca 
      cctgcagat ctttaa
    • >TC006162 RB
      atgtctctc ttcggctca ttaatgggc gatattgag 
      gaggatccg tttttcgga tctcacatg aatatgatg 
      cgccaaatg aacacaatg atgaattcc ttcttctcg 
      gaccctttt tcgatgggt ttcggtgat ttcgatcgc 
      ggtcaccgc atgagcaac tctcttatg cctttttca 
      atgccaatt atgccaaat ttcaaccgg ctgttgagc 
      ggctcgttg gactccctg ggggcgcac agttacagt 
      tccagcacc gtggttagc atgtcttcg gggcccgac 
      ggccgcccc caggtgtac aaagccacg tcgagcacg 
      cgcacagct cctgggggt attaaagag acccaaaag 
      acagtcact gacacacgg tcagggacc aagaaaatg 
      gccatcggg caccacatc ggcgaacgg gcccacatc 
      atagaaaag gagcagaat atgcacact ggggaccgt 
      gaggaaagg caggatttt atcaatttg gacgaggaa 
      gaggccgag gattttaat aaagagtgg gagactaag 
      actaggagt cgttcggag attcccagg atttgttcg 
      gggtctgtg cgaaaccga catgggtat ggggctcca 
      ggaggcatg ctggcgatc actggaggg cctagacgt 
      cggcaaaga cagtcaacc ccaatggtc tcgcctcgt 
      agaagtata agatcgtcg ccgttaact gtgccacag 
      gcgtcggct agctctagg actccttac agccccccg 
      ggacaagtc caaaatgtc aggaagagt aaaaatgtt 
      aagacggtg tcaggatca ccaccacca ccaccacct 
      gcagatctt taa
    • >TC006162 RC
      atgtctctc ttcggctca ttaatgggc gatattgag 
      gaggatccg tttttcgga tctcacatg aatatgatg 
      cgccaaatg aacacaatg atgaattcc ttcttctcg 
      gaccctttt tcgatgggt ttcggtgat ttcgatcgc 
      ggtcaccgc atgagcaac tctcttatg cctttttca 
      atgccaatt atgccaaat ttcaaccgg ctgttgagc 
      ggctcgttg gactccctg ggggcgcac agttacagt 
      tccagcacc gtggttagc atgtcttcg gggcccgac 
      ggccgcccc caggtgtac aaagccacg tcgagcacg 
      cgcacagct cctgggggt attaaagag acccaaaag 
      acagtcact gacacacgg tcagggacc aagaaaatg 
      gccatcggg caccacatc ggcgaacgg gcccacatc 
      atagaaaag gagcagaat atgcacact ggggaccgt 
      gaggaaagg caggatttt atcaatttg gacgaggaa 
      gaggccgag gattttaat aaagagtgg gagactaag 
      actaggagt cgttcggag attcccagg atttgttcg 
      gggtctgtg cgaaaccga catgggtat ggggctcca 
      ggaggcatg ctggcgatc actggaggg cctagctct 
      aggactcct tacagcccc ccgggacaa gtccaaaat 
      gtcaggaag agtaaaaat gttaagacg gtgtcagga 
      tcaccacca ccaccacca cctgcagat ctttaa
    mRNA sequence
    • >TC006162 RA
      ctttgattt tccgaacgc cgcagcgcc gcctggcgg 
      ccgtttcat tccccgcgc caagtgtca ctagtgtcg 
      ttcattagc aaagtgttt tgtgaatta ttgttgaga 
      aaagtgtta aaacctttc agaaaatgt ctctcttcg 
      gctcattaa tgggcgata ttgaggagg atccgtttt 
      tcggatctc acatgaata tgatgcgcc aaatgaaca 
      caatgatga attccttct tctcggacc ctttttcga 
      tgggtttcg gtgatttcg atcgcggtc accgcatga 
      gcaactctc ttatgcctt tttcaatgc caattatgc 
      caaatttca accggctgt tgagcggct cgttggact 
      ccctggggg cgcacagtt acagttcca gcaccgtgg 
      ttagcatgt cttcggggc ccgacggcc gcccccagg 
      tgtacaaag ccacgtcga gcacgcgca cagctcctg 
      ggggtatta aagagaccc aaaagacag tcactgaca 
      cacggtcag ggaccaaga aaatggcca tcgggcacc 
      acatcggcg aacgggccc acatcatag aaaaggagc 
      agaatatgc acactgggg accgtgagg aaaggcagg 
      attttatca atttggacg aggaagagg ccgaggatt 
      ttaataaag agtgggaga ctaagacta ggagtcgtt 
      cggagattc ccaggattt gttcggggt ctgtgcgaa 
      accgacatg ggtatgggg ctccaggag gcatgctgg 
      cgatcactg gagggccta gtagacgtc ggcaaagac 
      agtcaaccc caatggtct cgcctcgta gaagtataa 
      gatcgtcgc cgttaactg tgccacagg cgtcggcta 
      gctctagga ctccttaca gccccccgg gacaagtcc 
      aaaatgtca ggaagagta aaaatgtta agacggtgt 
      caggatcac caccaccac caccacctg cagatcttt 
      aaatttgtt caatgttac cttctctga ctaagaagt 
      aattatatt ttttgtaat gggaaatta tttattttt 
      attctagac aagaaaata gtacaatta ttgtcatgg 
      tcgtttgtt tcacacttg cgtgtcaat tcctaaact 
      caaacataa ttattaatt gtatgacgt atgtattgt 
      aacgttgtt gggtgattt cattgtaaa acctagagt 
      tcctatatt gggagag
    • >TC006162 RB
      ctttgattt tccgaacgc cgcagcgcc gcctggcgg 
      ccgtttcat tccccgcgc caagtgtca ctagtgtcg 
      ttcattagc aaagtgttt tgtgaatta ttgttgaga 
      aaagtgtta aaacctttc agaaaatgt ctctcttcg 
      gctcattaa tgggcgata ttgaggagg atccgtttt 
      tcggatctc acatgaata tgatgcgcc aaatgaaca 
      caatgatga attccttct tctcggacc ctttttcga 
      tgggtttcg gtgatttcg atcgcggtc accgcatga 
      gcaactctc ttatgcctt tttcaatgc caattatgc 
      caaatttca accggctgt tgagcggct cgttggact 
      ccctggggg cgcacagtt acagttcca gcaccgtgg 
      ttagcatgt cttcggggc ccgacggcc gcccccagg 
      tgtacaaag ccacgtcga gcacgcgca cagctcctg 
      ggggtatta aagagaccc aaaagacag tcactgaca 
      cacggtcag ggaccaaga aaatggcca tcgggcacc 
      acatcggcg aacgggccc acatcatag aaaaggagc 
      agaatatgc acactgggg accgtgagg aaaggcagg 
      attttatca atttggacg aggaagagg ccgaggatt 
      ttaataaag agtgggaga ctaagacta ggagtcgtt 
      cggagattc ccaggattt gttcggggt ctgtgcgaa 
      accgacatg ggtatgggg ctccaggag gcatgctgg 
      cgatcactg gagggccta gacgtcggc aaagacagt 
      caaccccaa tggtctcgc ctcgtagaa gtataagat 
      cgtcgccgt taactgtgc cacaggcgt cggctagct 
      ctaggactc cttacagcc ccccgggac aagtccaaa 
      atgtcagga agagtaaaa atgttaaga cggtgtcag 
      gatcaccac caccaccac cacctgcag atctttaaa 
      tttgttcaa tgttacctt ctctgacta agaagtaat 
      tatattttt tgtaatggg aaattattt atttttatt 
      ctagacaag aaaatagta caattattg tcatggtcg 
      tttgtttca cacttgcgt gtcaattcc taaactcaa 
      acataatta ttaattgta tgacgtatg tattgtaac 
      gttgttggg tgatttcat tgtaaaacc tagagttcc 
      tatattggg agag
    • >TC006162 RC
      ctttgattt tccgaacgc cgcagcgcc gcctggcgg 
      ccgtttcat tccccgcgc caagtgtca ctagtgtcg 
      ttcattagc aaagtgttt tgtgaatta ttgttgaga 
      aaagtgtta aaacctttc agaaaatgt ctctcttcg 
      gctcattaa tgggcgata ttgaggagg atccgtttt 
      tcggatctc acatgaata tgatgcgcc aaatgaaca 
      caatgatga attccttct tctcggacc ctttttcga 
      tgggtttcg gtgatttcg atcgcggtc accgcatga 
      gcaactctc ttatgcctt tttcaatgc caattatgc 
      caaatttca accggctgt tgagcggct cgttggact 
      ccctggggg cgcacagtt acagttcca gcaccgtgg 
      ttagcatgt cttcggggc ccgacggcc gcccccagg 
      tgtacaaag ccacgtcga gcacgcgca cagctcctg 
      ggggtatta aagagaccc aaaagacag tcactgaca 
      cacggtcag ggaccaaga aaatggcca tcgggcacc 
      acatcggcg aacgggccc acatcatag aaaaggagc 
      agaatatgc acactgggg accgtgagg aaaggcagg 
      attttatca atttggacg aggaagagg ccgaggatt 
      ttaataaag agtgggaga ctaagacta ggagtcgtt 
      cggagattc ccaggattt gttcggggt ctgtgcgaa 
      accgacatg ggtatgggg ctccaggag gcatgctgg 
      cgatcactg gagggccta gctctagga ctccttaca 
      gccccccgg gacaagtcc aaaatgtca ggaagagta 
      aaaatgtta agacggtgt caggatcac caccaccac 
      caccacctg cagatcttt aaatttgtt caatgttac 
      cttctctga ctaagaagt aattatatt ttttgtaat 
      gggaaatta tttattttt attctagac aagaaaata 
      gtacaatta ttgtcatgg tcgtttgtt tcacacttg 
      cgtgtcaat tcctaaact caaacataa ttattaatt 
      gtatgacgt atgtattgt aacgttgtt gggtgattt 
      cattgtaaa acctagagt tcctatatt gggagag