Status
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Gene information
General information
TC033963
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006004,TC006006
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 3139409..3308826
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033963
    iBeetle screen iB_02892
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033963 RA
      MMAGREVLY LVLLAASVF IGHGYSSPS PIKRTDPKK 
      DHCNKTVEI YEDVASPEV TSENFGKPM TCWYRFRSF 
      RGTPKDWIL RIKFNKFKV GVLLNASHC EGGYMQIVD 
      GNAKTDVSN RKEPGIFCG ESEQPQTFM SETSFVKVV 
      FHSDNFTDQ TYFSFDTRA EQQLVVYLR YGQHPELYP 
      NRRGEVVQG SYCERIFRD CRLQTCYVQ SPAYPGVYP 
      RNLHCRYYL NTRLPFIKL YIENEEFNI DGQRCENIM 
      TCPMRPISS GQENCPYDY IKIYDGKNE LSPVIGTFC 
      GMGKFPYSI IGTSQDLFV EFVSSPAGP LLNTGFHFN 
      VGNWPGHVD TAGSRNGTC DWLLSSESL RASGDSEGI 
      FLSVAHWYP PHTSCTYLM QGEEGEIVR LYFPSFRIN 
      RIESPIQPI EGDCGESLT LYDSSWPDD SKIIKTFCD 
      TFSKAMEKH DFVSTGNAL FVRFESKTG SYSGSSLYY 
      WAHYDFFNN TKLGEPVPG KPCDEVFAS WRQTRGWLR 
      SPLNTLVYK QTQSSEDVQ CLYRFVTDR RLFARVILT 
      ITNIDFKEN PYNQAPCSN CLEDRVDKL VIWEPLPPG 
      ANTSYPPIP LTKAAQSGN VTCLCSKHT SSTQIISRG 
      EQLNLQLIV DSAHSALSY FKHNSPLFE ATYEFVHGP 
      LCGPPIIQP LLNGEIHYP YYEAIGYVE PPKSIRCIW 
      EIKVNRERD LWLHFDRIK FSSKACDDG IVDIFLKGR 
      LEPYLSICG ENVSLVKEL PVLSAAELS PDGTEPGVT 
      IQFIGRTSP TRAAFTIAW TELFHLPRN QDGSLMSSR 
      LTGGGNVED DEGCGFVCP GEAGLCIAA GLVCNGVIN 
      CPNVTDHKG TTFSDESAE LCVSEHSPE INWIYVGAA 
      AVSVLLLLC CFCVCLCRR CCRYCCSDD
    • >TC033963 RB
      MEQHLHQFK VIAIIVMEA ETYMGYNLV QVLYDVGLM 
      DARNGLLLQ VHYSYNFYI FTPKEHSNF KPMEFSLTD 
      PKKDHCNKT VEIYEDVAS PEVTSENFG KPMTCWYRF 
      RSFRGTPKD WILRIKFNK FKVGVLLNA SHCEGGYMQ 
      IVDGNAKTD VSNRKEPGI FCGESEQPQ TFMSETSFV 
      KVVFHSDNF TDQTYFSFD TRAEQQLVV YLRYGQHPE 
      LYPNRRGEV VQGSYCERI FRDCRLQTC YVQSPAYPG 
      VYPRNLHCR YYLNTRLPF IKLYIENEE FNIDGQRCE 
      NIMTCPMRP ISSGQENCP YDYIKIYDG KNELSPVIG 
      TFCGMGKFP YSIIGTSQD LFVEFVSSP AGPLLNTGF 
      HFNVGNWPG HVDTAGSRN GTCDWLLSS ESLRASGDS 
      EGIFLSVAH WYPPHTSCT YLMQGEEGE IVRLYFPSF 
      RINRIESPI QPIEGDCGE SLTLYDSSW PDDSKIIKT 
      FCDTFSKAM EKHDFVSTG NALFVRFES KTGSYSGSS 
      LYYWAHYDF FNNTKLGEP VPGKPCDEV FASWRQTRG 
      WLRSPLNTL VYKQTQSSE DVQCLYRFV TDRRLFARV 
      ILTITNIDF KENPYNQAP CSNCLEDRV DKLVIWEPL 
      PPGANTSYP PIPLTKAAQ SGNVTCLCS KHTSSTQII 
      SRGEQLNLQ LIVDSAHSA LSYFKHNSP LFEATYEFV 
      HGPLCGPPI IQPLLNGEI HYPYYEAIG YVEPPKSIR 
      CIWEIKVNR ERDLWLHFD RIKFSSKAC DDGIVDIFL 
      KGRLEPYLS ICGENVSLV KELPVLSAA ELSPDGTEP 
      GVTIQFIGR TSPTRAAFT IAWTELFHL PRNQDGSLM 
      SSRLTGGGN VEDDEGCGF VCPGEAGLC IAAGLVCNG 
      VINCPNVTD HKGTTFSDE SAELCVSEH SPEINWIYV 
      GAAAVSVLL LLCCFCVCL CRRCCRYCC SDD
    CDS sequence
    • >TC033963 RA
      atgatggcc gggagggaa gtgctgtat ctcgtcttg 
      ctggccgcc agcgtcttc ataggacac ggctattcg 
      agtccctcg ccaatcaaa agaactgat ccgaagaaa 
      gaccactgc aacaaaacg gtggaaatt tacgaggac 
      gtcgcaagt ccggaagtg acgagtgag aacttcggg 
      aaaccgatg acatgttgg tacaggttt cggtcgttc 
      cgcggaact cccaaagat tggattctt cggataaag 
      ttcaacaaa ttcaaagtg ggtgttttg ctcaacgct 
      agccactgc gagggagga tatatgcag atcgtcgat 
      ggcaacgca aagacggac gtctcaaac cgcaaagag 
      cccggcatt ttttgcggt gagagtgaa caacctcaa 
      actttcatg tcggaaacg tctttcgtg aaggttgtg 
      tttcatagc gataatttc accgaccaa acttacttt 
      agtttcgat acgcgagcc gaacaacaa ctcgttgta 
      tatttgagg tatggccag cacccggaa ctttatcca 
      aatagacga ggggaggtc gttcagggg tcttactgc 
      gagaggata ttccgggac tgccgcctt caaacttgt 
      tacgtgcaa tctccggcc tatcccgga gtctatccc 
      cggaatttg cactgcaga tattacctg aacacaaga 
      ctgcctttc atcaagctg tacatcgag aacgaagaa 
      ttcaacatc gacggacag cgatgcgaa aatatcatg 
      acatgtccc atgaggccg atatcctca ggccaggag 
      aattgcccc tacgactac ataaaaatc tacgacgga 
      aagaacgag ttgagtcct gttattgga acgttttgc 
      gggatggga aagttcccg tactccatt ataggcaca 
      tctcaggac cttttcgtg gagtttgtt tcgtcgccg 
      gcaggccct ttgctcaac actggtttc cacttcaac 
      gtcggaaac tggccaggc cacgtggat acggccggg 
      agtcgaaac ggcacctgt gactggctc ctgtcctcc 
      gagagtctt cgagcatct ggagattcc gaaggaatt 
      ttcctctcc gtagcccac tggtacccc ccacatacc 
      agctgtaca tatctcatg caaggggaa gagggcgaa 
      atcgtccga ctatatttc cccagtttt cggataaac 
      cgaatcgag tctccaatc cagccaatt gaaggcgac 
      tgtggcgag tcgctcact ctttacgac tcctcctgg 
      cctgacgac tcaaaaatc atcaagact ttctgtgat 
      accttctcc aaagcaatg gaaaagcac gatttcgtc 
      tcaaccgga aacgccctc ttcgttcgc ttcgagagc 
      aaaaccggg agttacagc ggctcgtct ttgtactat 
      tgggcccat tacgatttt ttcaacaac acgaaattg 
      ggcgaacca gtgcctggc aaaccctgc gatgaagtg 
      ttcgcttcg tggaggcaa actcgaggc tggttgagg 
      tctccgctg aacactttg gtttacaag cagacacag 
      tcgagtgaa gatgtgcaa tgtttgtac cgttttgtt 
      accgatcgg agacttttc gctcgtgtt attttgact 
      ataacgaac atcgatttc aaggagaat ccatacaat 
      caagcgcct tgctcaaat tgtttggag gatcgagtt 
      gataagttg gtaatttgg gagcctctt cctccgggg 
      gctaacacc tcgtatccg ccgataccg ttgaccaag 
      gcggcccaa agtggcaat gtgacttgc ttgtgtagc 
      aagcacact tcttcgact caaatcata tcaagagga 
      gaacaactt aatcttcag ctgattgtg gatagtgcg 
      cattcggcg ttgagctac ttcaaacac aattcgccg 
      ctttttgaa gccacgtat gagtttgtg cacgggccg 
      ctgtgtggg cctccaatt attcaacca cttctaaat 
      ggtgaaatc cactaccct tactacgaa gccatcggc 
      tacgtggag cctcccaaa tcaatccgc tgcatctgg 
      gagattaaa gtaaaccga gagcgcgac ctttggttg 
      cacttcgac cgcattaaa ttctcctca aaggcttgc 
      gacgacggg atagtggac atcttcctc aaaggtcgt 
      ctcgagcca tacttatcg atctgcggc gagaacgtt 
      tctctcgtc aaagaactg ccagtgttg tcagcggcc 
      gaactaagt cctgatgga actgaaccg ggagttaca 
      atccagttc atcgggaga acatcacca acgagggca 
      gctttcact atagcgtgg acggagttg ttccatttg 
      ccgaggaat caagacgga agtcttatg tcttcgaga 
      cttacaggc ggagggaat gtcgaagac gatgaggga 
      tgcgggttt gtgtgtccg ggagaggca gggttgtgt 
      atagctgcg ggacttgtt tgtaatgga gtgattaat 
      tgcccgaat gttaccgat cacaaaggt accaccttt 
      agtgacgaa tctgcggag ttgtgcgtg tcggaacac 
      agccccgag atcaattgg atttacgtg ggggcggcg 
      gcagtgtca gtgttactc ctcctgtgt tgtttttgt 
      gtctgttta tgcaggagg tgttgtaga tactgttgc 
      agcgacgac taa
    • >TC033963 RB
      atggaacaa catctgcat cagttcaag gttatagcc 
      attattgtg atggaagct gagacttat atgggatat 
      aatttagtt caagttttg tacgatgtg ggattaatg 
      gatgcgagg aacggctta ctgctgcaa gttcactac 
      agctacaac ttttacatt tttacgccg aaagagcat 
      tcaaatttt aaaccgatg gaatttagc ttaactgat 
      ccgaagaaa gaccactgc aacaaaacg gtggaaatt 
      tacgaggac gtcgcaagt ccggaagtg acgagtgag 
      aacttcggg aaaccgatg acatgttgg tacaggttt 
      cggtcgttc cgcggaact cccaaagat tggattctt 
      cggataaag ttcaacaaa ttcaaagtg ggtgttttg 
      ctcaacgct agccactgc gagggagga tatatgcag 
      atcgtcgat ggcaacgca aagacggac gtctcaaac 
      cgcaaagag cccggcatt ttttgcggt gagagtgaa 
      caacctcaa actttcatg tcggaaacg tctttcgtg 
      aaggttgtg tttcatagc gataatttc accgaccaa 
      acttacttt agtttcgat acgcgagcc gaacaacaa 
      ctcgttgta tatttgagg tatggccag cacccggaa 
      ctttatcca aatagacga ggggaggtc gttcagggg 
      tcttactgc gagaggata ttccgggac tgccgcctt 
      caaacttgt tacgtgcaa tctccggcc tatcccgga 
      gtctatccc cggaatttg cactgcaga tattacctg 
      aacacaaga ctgcctttc atcaagctg tacatcgag 
      aacgaagaa ttcaacatc gacggacag cgatgcgaa 
      aatatcatg acatgtccc atgaggccg atatcctca 
      ggccaggag aattgcccc tacgactac ataaaaatc 
      tacgacgga aagaacgag ttgagtcct gttattgga 
      acgttttgc gggatggga aagttcccg tactccatt 
      ataggcaca tctcaggac cttttcgtg gagtttgtt 
      tcgtcgccg gcaggccct ttgctcaac actggtttc 
      cacttcaac gtcggaaac tggccaggc cacgtggat 
      acggccggg agtcgaaac ggcacctgt gactggctc 
      ctgtcctcc gagagtctt cgagcatct ggagattcc 
      gaaggaatt ttcctctcc gtagcccac tggtacccc 
      ccacatacc agctgtaca tatctcatg caaggggaa 
      gagggcgaa atcgtccga ctatatttc cccagtttt 
      cggataaac cgaatcgag tctccaatc cagccaatt 
      gaaggcgac tgtggcgag tcgctcact ctttacgac 
      tcctcctgg cctgacgac tcaaaaatc atcaagact 
      ttctgtgat accttctcc aaagcaatg gaaaagcac 
      gatttcgtc tcaaccgga aacgccctc ttcgttcgc 
      ttcgagagc aaaaccggg agttacagc ggctcgtct 
      ttgtactat tgggcccat tacgatttt ttcaacaac 
      acgaaattg ggcgaacca gtgcctggc aaaccctgc 
      gatgaagtg ttcgcttcg tggaggcaa actcgaggc 
      tggttgagg tctccgctg aacactttg gtttacaag 
      cagacacag tcgagtgaa gatgtgcaa tgtttgtac 
      cgttttgtt accgatcgg agacttttc gctcgtgtt 
      attttgact ataacgaac atcgatttc aaggagaat 
      ccatacaat caagcgcct tgctcaaat tgtttggag 
      gatcgagtt gataagttg gtaatttgg gagcctctt 
      cctccgggg gctaacacc tcgtatccg ccgataccg 
      ttgaccaag gcggcccaa agtggcaat gtgacttgc 
      ttgtgtagc aagcacact tcttcgact caaatcata 
      tcaagagga gaacaactt aatcttcag ctgattgtg 
      gatagtgcg cattcggcg ttgagctac ttcaaacac 
      aattcgccg ctttttgaa gccacgtat gagtttgtg 
      cacgggccg ctgtgtggg cctccaatt attcaacca 
      cttctaaat ggtgaaatc cactaccct tactacgaa 
      gccatcggc tacgtggag cctcccaaa tcaatccgc 
      tgcatctgg gagattaaa gtaaaccga gagcgcgac 
      ctttggttg cacttcgac cgcattaaa ttctcctca 
      aaggcttgc gacgacggg atagtggac atcttcctc 
      aaaggtcgt ctcgagcca tacttatcg atctgcggc 
      gagaacgtt tctctcgtc aaagaactg ccagtgttg 
      tcagcggcc gaactaagt cctgatgga actgaaccg 
      ggagttaca atccagttc atcgggaga acatcacca 
      acgagggca gctttcact atagcgtgg acggagttg 
      ttccatttg ccgaggaat caagacgga agtcttatg 
      tcttcgaga cttacaggc ggagggaat gtcgaagac 
      gatgaggga tgcgggttt gtgtgtccg ggagaggca 
      gggttgtgt atagctgcg ggacttgtt tgtaatgga 
      gtgattaat tgcccgaat gttaccgat cacaaaggt 
      accaccttt agtgacgaa tctgcggag ttgtgcgtg 
      tcggaacac agccccgag atcaattgg atttacgtg 
      ggggcggcg gcagtgtca gtgttactc ctcctgtgt 
      tgtttttgt gtctgttta tgcaggagg tgttgtaga 
      tactgttgc agcgacgac taa
    mRNA sequence
    • >TC033963 RA
      tgcgtcggt tcaccgtga gtgcaactt caatggtgt 
      cgaaagctc gattgcgat agtgccaat gatgtaatt 
      ggtgtgagt gtgagcttt tgctgcagc tttgctgtg 
      agttatgtg cttatgatg atttgtgcg cattgagaa 
      aaatgatgg ccgggaggg aagtgctgt atctcgtct 
      tgctggccg ccagcgtct tcataggac acggctatt 
      cgagtccct cgccaatca aaagaactg atccgaaga 
      aagaccact gcaacaaaa cggtggaaa tttacgagg 
      acgtcgcaa gtccggaag tgacgagtg agaacttcg 
      ggaaaccga tgacatgtt ggtacaggt ttcggtcgt 
      tccgcggaa ctcccaaag attggattc ttcggataa 
      agttcaaca aattcaaag tgggtgttt tgctcaacg 
      ctagccact gcgagggag gatatatgc agatcgtcg 
      atggcaacg caaagacgg acgtctcaa accgcaaag 
      agcccggca ttttttgcg gtgagagtg aacaacctc 
      aaactttca tgtcggaaa cgtctttcg tgaaggttg 
      tgtttcata gcgataatt tcaccgacc aaacttact 
      ttagtttcg atacgcgag ccgaacaac aactcgttg 
      tatatttga ggtatggcc agcacccgg aactttatc 
      caaatagac gaggggagg tcgttcagg ggtcttact 
      gcgagagga tattccggg actgccgcc ttcaaactt 
      gttacgtgc aatctccgg cctatcccg gagtctatc 
      cccggaatt tgcactgca gatattacc tgaacacaa 
      gactgcctt tcatcaagc tgtacatcg agaacgaag 
      aattcaaca tcgacggac agcgatgcg aaaatatca 
      tgacatgtc ccatgaggc cgatatcct caggccagg 
      agaattgcc cctacgact acataaaaa tctacgacg 
      gaaagaacg agttgagtc ctgttattg gaacgtttt 
      gcgggatgg gaaagttcc cgtactcca ttataggca 
      catctcagg accttttcg tggagtttg tttcgtcgc 
      cggcaggcc ctttgctca acactggtt tccacttca 
      acgtcggaa actggccag gccacgtgg atacggccg 
      ggagtcgaa acggcacct gtgactggc tcctgtcct 
      ccgagagtc ttcgagcat ctggagatt ccgaaggaa 
      ttttcctct ccgtagccc actggtacc ccccacata 
      ccagctgta catatctca tgcaagggg aagagggcg 
      aaatcgtcc gactatatt tccccagtt ttcggataa 
      accgaatcg agtctccaa tccagccaa ttgaaggcg 
      actgtggcg agtcgctca ctctttacg actcctcct 
      ggcctgacg actcaaaaa tcatcaaga ctttctgtg 
      ataccttct ccaaagcaa tggaaaagc acgatttcg 
      tctcaaccg gaaacgccc tcttcgttc gcttcgaga 
      gcaaaaccg ggagttaca gcggctcgt ctttgtact 
      attgggccc attacgatt ttttcaaca acacgaaat 
      tgggcgaac cagtgcctg gcaaaccct gcgatgaag 
      tgttcgctt cgtggaggc aaactcgag gctggttga 
      ggtctccgc tgaacactt tggtttaca agcagacac 
      agtcgagtg aagatgtgc aatgtttgt accgttttg 
      ttaccgatc ggagacttt tcgctcgtg ttattttga 
      ctataacga acatcgatt tcaaggaga atccataca 
      atcaagcgc cttgctcaa attgtttgg aggatcgag 
      ttgataagt tggtaattt gggagcctc ttcctccgg 
      gggctaaca cctcgtatc cgccgatac cgttgacca 
      aggcggccc aaagtggca atgtgactt gcttgtgta 
      gcaagcaca cttcttcga ctcaaatca tatcaagag 
      gagaacaac ttaatcttc agctgattg tggatagtg 
      cgcattcgg cgttgagct acttcaaac acaattcgc 
      cgctttttg aagccacgt atgagtttg tgcacgggc 
      cgctgtgtg ggcctccaa ttattcaac cacttctaa 
      atggtgaaa tccactacc cttactacg aagccatcg 
      gctacgtgg agcctccca aatcaatcc gctgcatct 
      gggagatta aagtaaacc gagagcgcg acctttggt 
      tgcacttcg accgcatta aattctcct caaaggctt 
      gcgacgacg ggatagtgg acatcttcc tcaaaggtc 
      gtctcgagc catacttat cgatctgcg gcgagaacg 
      tttctctcg tcaaagaac tgccagtgt tgtcagcgg 
      ccgaactaa gtcctgatg gaactgaac cgggagtta 
      caatccagt tcatcggga gaacatcac caacgaggg 
      cagctttca ctatagcgt ggacggagt tgttccatt 
      tgccgagga atcaagacg gaagtctta tgtcttcga 
      gacttacag gcggaggga atgtcgaag acgatgagg 
      gatgcgggt ttgtgtgtc cgggagagg cagggttgt 
      gtatagctg cgggacttg tttgtaatg gagtgatta 
      attgcccga atgttaccg atcacaaag gtaccacct 
      ttagtgacg aatctgcgg agttgtgcg tgtcggaac 
      acagccccg agatcaatt ggatttacg tgggggcgg 
      cggcagtgt cagtgttac tcctcctgt gttgttttt 
      gtgtctgtt tatgcagga ggtgttgta gatactgtt 
      gcagcgacg actaaagtt caattttac gaaaccgaa 
      gccttaatg aaccttttt aacgaaaaa atacaaact 
      aaaagttca agtcttgac tacgtgaca atccaacaa 
      taaattttc cttttgtaa aaaccgcaa tgtagtcca 
      agacttgac cttttaata gagacttaa agcgaagtg 
      aagtgcaca tcgatggaa ctttttggc aaagcactc 
      acgtttagt ttagggtct ttagagtac cgttgtatt 
      atttccaag agagatttg aaccttgca cagttttta 
      cgattcaac aaataaagc gatcatgtt ctaaaattt 
      gtttttgta aaataccta atgccaaaa attcgaaga 
      agtgtatat ttttagcga taagttttt aatttaaat 
      gatgtacat attatgtaa tactttcac atttccatg 
      tgataataa accattgtt ttaaaa
    • >TC033963 RB
      ccggatccg gagtcgcga ccggcttat tgcaggaat 
      gtttgggtg ttaccatta taaacagat gccggaggc 
      cgactagtt atggaacaa catctgcat cagttcaag 
      gttatagcc attattgtg atggaagct gagacttat 
      atgggatat aatttagtt caagttttg tacgatgtg 
      ggattaatg gatgcgagg aacggctta ctgctgcaa 
      gttcactac agctacaac ttttacatt tttacgccg 
      aaagagcat tcaaatttt aaaccgatg gaatttagc 
      ttaactgat ccgaagaaa gaccactgc aacaaaacg 
      gtggaaatt tacgaggac gtcgcaagt ccggaagtg 
      acgagtgag aacttcggg aaaccgatg acatgttgg 
      tacaggttt cggtcgttc cgcggaact cccaaagat 
      tggattctt cggataaag ttcaacaaa ttcaaagtg 
      ggtgttttg ctcaacgct agccactgc gagggagga 
      tatatgcag atcgtcgat ggcaacgca aagacggac 
      gtctcaaac cgcaaagag cccggcatt ttttgcggt 
      gagagtgaa caacctcaa actttcatg tcggaaacg 
      tctttcgtg aaggttgtg tttcatagc gataatttc 
      accgaccaa acttacttt agtttcgat acgcgagcc 
      gaacaacaa ctcgttgta tatttgagg tatggccag 
      cacccggaa ctttatcca aatagacga ggggaggtc 
      gttcagggg tcttactgc gagaggata ttccgggac 
      tgccgcctt caaacttgt tacgtgcaa tctccggcc 
      tatcccgga gtctatccc cggaatttg cactgcaga 
      tattacctg aacacaaga ctgcctttc atcaagctg 
      tacatcgag aacgaagaa ttcaacatc gacggacag 
      cgatgcgaa aatatcatg acatgtccc atgaggccg 
      atatcctca ggccaggag aattgcccc tacgactac 
      ataaaaatc tacgacgga aagaacgag ttgagtcct 
      gttattgga acgttttgc gggatggga aagttcccg 
      tactccatt ataggcaca tctcaggac cttttcgtg 
      gagtttgtt tcgtcgccg gcaggccct ttgctcaac 
      actggtttc cacttcaac gtcggaaac tggccaggc 
      cacgtggat acggccggg agtcgaaac ggcacctgt 
      gactggctc ctgtcctcc gagagtctt cgagcatct 
      ggagattcc gaaggaatt ttcctctcc gtagcccac 
      tggtacccc ccacatacc agctgtaca tatctcatg 
      caaggggaa gagggcgaa atcgtccga ctatatttc 
      cccagtttt cggataaac cgaatcgag tctccaatc 
      cagccaatt gaaggcgac tgtggcgag tcgctcact 
      ctttacgac tcctcctgg cctgacgac tcaaaaatc 
      atcaagact ttctgtgat accttctcc aaagcaatg 
      gaaaagcac gatttcgtc tcaaccgga aacgccctc 
      ttcgttcgc ttcgagagc aaaaccggg agttacagc 
      ggctcgtct ttgtactat tgggcccat tacgatttt 
      ttcaacaac acgaaattg ggcgaacca gtgcctggc 
      aaaccctgc gatgaagtg ttcgcttcg tggaggcaa 
      actcgaggc tggttgagg tctccgctg aacactttg 
      gtttacaag cagacacag tcgagtgaa gatgtgcaa 
      tgtttgtac cgttttgtt accgatcgg agacttttc 
      gctcgtgtt attttgact ataacgaac atcgatttc 
      aaggagaat ccatacaat caagcgcct tgctcaaat 
      tgtttggag gatcgagtt gataagttg gtaatttgg 
      gagcctctt cctccgggg gctaacacc tcgtatccg 
      ccgataccg ttgaccaag gcggcccaa agtggcaat 
      gtgacttgc ttgtgtagc aagcacact tcttcgact 
      caaatcata tcaagagga gaacaactt aatcttcag 
      ctgattgtg gatagtgcg cattcggcg ttgagctac 
      ttcaaacac aattcgccg ctttttgaa gccacgtat 
      gagtttgtg cacgggccg ctgtgtggg cctccaatt 
      attcaacca cttctaaat ggtgaaatc cactaccct 
      tactacgaa gccatcggc tacgtggag cctcccaaa 
      tcaatccgc tgcatctgg gagattaaa gtaaaccga 
      gagcgcgac ctttggttg cacttcgac cgcattaaa 
      ttctcctca aaggcttgc gacgacggg atagtggac 
      atcttcctc aaaggtcgt ctcgagcca tacttatcg 
      atctgcggc gagaacgtt tctctcgtc aaagaactg 
      ccagtgttg tcagcggcc gaactaagt cctgatgga 
      actgaaccg ggagttaca atccagttc atcgggaga 
      acatcacca acgagggca gctttcact atagcgtgg 
      acggagttg ttccatttg ccgaggaat caagacgga 
      agtcttatg tcttcgaga cttacaggc ggagggaat 
      gtcgaagac gatgaggga tgcgggttt gtgtgtccg 
      ggagaggca gggttgtgt atagctgcg ggacttgtt 
      tgtaatgga gtgattaat tgcccgaat gttaccgat 
      cacaaaggt accaccttt agtgacgaa tctgcggag 
      ttgtgcgtg tcggaacac agccccgag atcaattgg 
      atttacgtg ggggcggcg gcagtgtca gtgttactc 
      ctcctgtgt tgtttttgt gtctgttta tgcaggagg 
      tgttgtaga tactgttgc agcgacgac taaagttca 
      attttacga aaccgaagc cttaatgaa cctttttaa 
      cgaaaaaat acaaactaa aagttcaag tcttgacta 
      cgtgacaat ccaacaata aattttcct tttgtaaaa 
      accgcaatg tagtccaag acttgacct tttaataga 
      gacttaaag cgaagtgaa gtgcacatc gatggaact 
      ttttggcaa agcactcac gtttagttt agggtcttt 
      agagtaccg ttgtattat ttccaagag agatttgaa 
      ccttgcaca gtttttacg attcaacaa ataaagcga 
      tcatgttct aaaatttgt ttttgtaaa atacctaat 
      gccaaaaat tcgaagaag tgtatattt ttagcgata 
      agtttttaa tttaaatga tgtacatat tatgtaata 
      ctttcacat ttccatgtg ataataaac cattgtttt 
      aaaa