| General information | |||
| TC033963
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 3444831..3614248 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC033963 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02892 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC033963 RA
MMAGREVLY LVLLAASVF IGHGYSSPS PIKRTDPKK DHCNKTVEI YEDVASPEV TSENFGKPM TCWYRFRSF RGTPKDWIL RIKFNKFKV GVLLNASHC EGGYMQIVD GNAKTDVSN RKEPGIFCG ESEQPQTFM SETSFVKVV FHSDNFTDQ TYFSFDTRA EQQLVVYLR YGQHPELYP NRRGEVVQG SYCERIFRD CRLQTCYVQ SPAYPGVYP RNLHCRYYL NTRLPFIKL YIENEEFNI DGQRCENIM TCPMRPISS GQENCPYDY IKIYDGKNE LSPVIGTFC GMGKFPYSI IGTSQDLFV EFVSSPAGP LLNTGFHFN VGNWPGHVD TAGSRNGTC DWLLSSESL RASGDSEGI FLSVAHWYP PHTSCTYLM QGEEGEIVR LYFPSFRIN RIESPIQPI EGDCGESLT LYDSSWPDD SKIIKTFCD TFSKAMEKH DFVSTGNAL FVRFESKTG SYSGSSLYY WAHYDFFNN TKLGEPVPG KPCDEVFAS WRQTRGWLR SPLNTLVYK QTQSSEDVQ CLYRFVTDR RLFARVILT ITNIDFKEN PYNQAPCSN CLEDRVDKL VIWEPLPPG ANTSYPPIP LTKAAQSGN VTCLCSKHT SSTQIISRG EQLNLQLIV DSAHSALSY FKHNSPLFE ATYEFVHGP LCGPPIIQP LLNGEIHYP YYEAIGYVE PPKSIRCIW EIKVNRERD LWLHFDRIK FSSKACDDG IVDIFLKGR LEPYLSICG ENVSLVKEL PVLSAAELS PDGTEPGVT IQFIGRTSP TRAAFTIAW TELFHLPRN QDGSLMSSR LTGGGNVED DEGCGFVCP GEAGLCIAA GLVCNGVIN CPNVTDHKG TTFSDESAE LCVSEHSPE INWIYVGAA AVSVLLLLC CFCVCLCRR CCRYCCSDD
- >TC033963 RB
MEQHLHQFK VIAIIVMEA ETYMGYNLV QVLYDVGLM DARNGLLLQ VHYSYNFYI FTPKEHSNF KPMEFSLTD PKKDHCNKT VEIYEDVAS PEVTSENFG KPMTCWYRF RSFRGTPKD WILRIKFNK FKVGVLLNA SHCEGGYMQ IVDGNAKTD VSNRKEPGI FCGESEQPQ TFMSETSFV KVVFHSDNF TDQTYFSFD TRAEQQLVV YLRYGQHPE LYPNRRGEV VQGSYCERI FRDCRLQTC YVQSPAYPG VYPRNLHCR YYLNTRLPF IKLYIENEE FNIDGQRCE NIMTCPMRP ISSGQENCP YDYIKIYDG KNELSPVIG TFCGMGKFP YSIIGTSQD LFVEFVSSP AGPLLNTGF HFNVGNWPG HVDTAGSRN GTCDWLLSS ESLRASGDS EGIFLSVAH WYPPHTSCT YLMQGEEGE IVRLYFPSF RINRIESPI QPIEGDCGE SLTLYDSSW PDDSKIIKT FCDTFSKAM EKHDFVSTG NALFVRFES KTGSYSGSS LYYWAHYDF FNNTKLGEP VPGKPCDEV FASWRQTRG WLRSPLNTL VYKQTQSSE DVQCLYRFV TDRRLFARV ILTITNIDF KENPYNQAP CSNCLEDRV DKLVIWEPL PPGANTSYP PIPLTKAAQ SGNVTCLCS KHTSSTQII SRGEQLNLQ LIVDSAHSA LSYFKHNSP LFEATYEFV HGPLCGPPI IQPLLNGEI HYPYYEAIG YVEPPKSIR CIWEIKVNR ERDLWLHFD RIKFSSKAC DDGIVDIFL KGRLEPYLS ICGENVSLV KELPVLSAA ELSPDGTEP GVTIQFIGR TSPTRAAFT IAWTELFHL PRNQDGSLM SSRLTGGGN VEDDEGCGF VCPGEAGLC IAAGLVCNG VINCPNVTD HKGTTFSDE SAELCVSEH SPEINWIYV GAAAVSVLL LLCCFCVCL CRRCCRYCC SDD
- >TC033963 RA
atgatggcc gggagggaa gtgctgtat ctcgtcttg ctggccgcc agcgtcttc ataggacac ggctattcg agtccctcg ccaatcaaa agaactgat ccgaagaaa gaccactgc aacaaaacg gtggaaatt tacgaggac gtcgcaagt ccggaagtg acgagtgag aacttcggg aaaccgatg acatgttgg tacaggttt cggtcgttc cgcggaact cccaaagat tggattctt cggataaag ttcaacaaa ttcaaagtg ggtgttttg ctcaacgct agccactgc gagggagga tatatgcag atcgtcgat ggcaacgca aagacggac gtctcaaac cgcaaagag cccggcatt ttttgcggt gagagtgaa caacctcaa actttcatg tcggaaacg tctttcgtg aaggttgtg tttcatagc gataatttc accgaccaa acttacttt agtttcgat acgcgagcc gaacaacaa ctcgttgta tatttgagg tatggccag cacccggaa ctttatcca aatagacga ggggaggtc gttcagggg tcttactgc gagaggata ttccgggac tgccgcctt caaacttgt tacgtgcaa tctccggcc tatcccgga gtctatccc cggaatttg cactgcaga tattacctg aacacaaga ctgcctttc atcaagctg tacatcgag aacgaagaa ttcaacatc gacggacag cgatgcgaa aatatcatg acatgtccc atgaggccg atatcctca ggccaggag aattgcccc tacgactac ataaaaatc tacgacgga aagaacgag ttgagtcct gttattgga acgttttgc gggatggga aagttcccg tactccatt ataggcaca tctcaggac cttttcgtg gagtttgtt tcgtcgccg gcaggccct ttgctcaac actggtttc cacttcaac gtcggaaac tggccaggc cacgtggat acggccggg agtcgaaac ggcacctgt gactggctc ctgtcctcc gagagtctt cgagcatct ggagattcc gaaggaatt ttcctctcc gtagcccac tggtacccc ccacatacc agctgtaca tatctcatg caaggggaa gagggcgaa atcgtccga ctatatttc cccagtttt cggataaac cgaatcgag tctccaatc cagccaatt gaaggcgac tgtggcgag tcgctcact ctttacgac tcctcctgg cctgacgac tcaaaaatc atcaagact ttctgtgat accttctcc aaagcaatg gaaaagcac gatttcgtc tcaaccgga aacgccctc ttcgttcgc ttcgagagc aaaaccggg agttacagc ggctcgtct ttgtactat tgggcccat tacgatttt ttcaacaac acgaaattg ggcgaacca gtgcctggc aaaccctgc gatgaagtg ttcgcttcg tggaggcaa actcgaggc tggttgagg tctccgctg aacactttg gtttacaag cagacacag tcgagtgaa gatgtgcaa tgtttgtac cgttttgtt accgatcgg agacttttc gctcgtgtt attttgact ataacgaac atcgatttc aaggagaat ccatacaat caagcgcct tgctcaaat tgtttggag gatcgagtt gataagttg gtaatttgg gagcctctt cctccgggg gctaacacc tcgtatccg ccgataccg ttgaccaag gcggcccaa agtggcaat gtgacttgc ttgtgtagc aagcacact tcttcgact caaatcata tcaagagga gaacaactt aatcttcag ctgattgtg gatagtgcg cattcggcg ttgagctac ttcaaacac aattcgccg ctttttgaa gccacgtat gagtttgtg cacgggccg ctgtgtggg cctccaatt attcaacca cttctaaat ggtgaaatc cactaccct tactacgaa gccatcggc tacgtggag cctcccaaa tcaatccgc tgcatctgg gagattaaa gtaaaccga gagcgcgac ctttggttg cacttcgac cgcattaaa ttctcctca aaggcttgc gacgacggg atagtggac atcttcctc aaaggtcgt ctcgagcca tacttatcg atctgcggc gagaacgtt tctctcgtc aaagaactg ccagtgttg tcagcggcc gaactaagt cctgatgga actgaaccg ggagttaca atccagttc atcgggaga acatcacca acgagggca gctttcact atagcgtgg acggagttg ttccatttg ccgaggaat caagacgga agtcttatg tcttcgaga cttacaggc ggagggaat gtcgaagac gatgaggga tgcgggttt gtgtgtccg ggagaggca gggttgtgt atagctgcg ggacttgtt tgtaatgga gtgattaat tgcccgaat gttaccgat cacaaaggt accaccttt agtgacgaa tctgcggag ttgtgcgtg tcggaacac agccccgag atcaattgg atttacgtg ggggcggcg gcagtgtca gtgttactc ctcctgtgt tgtttttgt gtctgttta tgcaggagg tgttgtaga tactgttgc agcgacgac taa
- >TC033963 RB
atggaacaa catctgcat cagttcaag gttatagcc attattgtg atggaagct gagacttat atgggatat aatttagtt caagttttg tacgatgtg ggattaatg gatgcgagg aacggctta ctgctgcaa gttcactac agctacaac ttttacatt tttacgccg aaagagcat tcaaatttt aaaccgatg gaatttagc ttaactgat ccgaagaaa gaccactgc aacaaaacg gtggaaatt tacgaggac gtcgcaagt ccggaagtg acgagtgag aacttcggg aaaccgatg acatgttgg tacaggttt cggtcgttc cgcggaact cccaaagat tggattctt cggataaag ttcaacaaa ttcaaagtg ggtgttttg ctcaacgct agccactgc gagggagga tatatgcag atcgtcgat ggcaacgca aagacggac gtctcaaac cgcaaagag cccggcatt ttttgcggt gagagtgaa caacctcaa actttcatg tcggaaacg tctttcgtg aaggttgtg tttcatagc gataatttc accgaccaa acttacttt agtttcgat acgcgagcc gaacaacaa ctcgttgta tatttgagg tatggccag cacccggaa ctttatcca aatagacga ggggaggtc gttcagggg tcttactgc gagaggata ttccgggac tgccgcctt caaacttgt tacgtgcaa tctccggcc tatcccgga gtctatccc cggaatttg cactgcaga tattacctg aacacaaga ctgcctttc atcaagctg tacatcgag aacgaagaa ttcaacatc gacggacag cgatgcgaa aatatcatg acatgtccc atgaggccg atatcctca ggccaggag aattgcccc tacgactac ataaaaatc tacgacgga aagaacgag ttgagtcct gttattgga acgttttgc gggatggga aagttcccg tactccatt ataggcaca tctcaggac cttttcgtg gagtttgtt tcgtcgccg gcaggccct ttgctcaac actggtttc cacttcaac gtcggaaac tggccaggc cacgtggat acggccggg agtcgaaac ggcacctgt gactggctc ctgtcctcc gagagtctt cgagcatct ggagattcc gaaggaatt ttcctctcc gtagcccac tggtacccc ccacatacc agctgtaca tatctcatg caaggggaa gagggcgaa atcgtccga ctatatttc cccagtttt cggataaac cgaatcgag tctccaatc cagccaatt gaaggcgac tgtggcgag tcgctcact ctttacgac tcctcctgg cctgacgac tcaaaaatc atcaagact ttctgtgat accttctcc aaagcaatg gaaaagcac gatttcgtc tcaaccgga aacgccctc ttcgttcgc ttcgagagc aaaaccggg agttacagc ggctcgtct ttgtactat tgggcccat tacgatttt ttcaacaac acgaaattg ggcgaacca gtgcctggc aaaccctgc gatgaagtg ttcgcttcg tggaggcaa actcgaggc tggttgagg tctccgctg aacactttg gtttacaag cagacacag tcgagtgaa gatgtgcaa tgtttgtac cgttttgtt accgatcgg agacttttc gctcgtgtt attttgact ataacgaac atcgatttc aaggagaat ccatacaat caagcgcct tgctcaaat tgtttggag gatcgagtt gataagttg gtaatttgg gagcctctt cctccgggg gctaacacc tcgtatccg ccgataccg ttgaccaag gcggcccaa agtggcaat gtgacttgc ttgtgtagc aagcacact tcttcgact caaatcata tcaagagga gaacaactt aatcttcag ctgattgtg gatagtgcg cattcggcg ttgagctac ttcaaacac aattcgccg ctttttgaa gccacgtat gagtttgtg cacgggccg ctgtgtggg cctccaatt attcaacca cttctaaat ggtgaaatc cactaccct tactacgaa gccatcggc tacgtggag cctcccaaa tcaatccgc tgcatctgg gagattaaa gtaaaccga gagcgcgac ctttggttg cacttcgac cgcattaaa ttctcctca aaggcttgc gacgacggg atagtggac atcttcctc aaaggtcgt ctcgagcca tacttatcg atctgcggc gagaacgtt tctctcgtc aaagaactg ccagtgttg tcagcggcc gaactaagt cctgatgga actgaaccg ggagttaca atccagttc atcgggaga acatcacca acgagggca gctttcact atagcgtgg acggagttg ttccatttg ccgaggaat caagacgga agtcttatg tcttcgaga cttacaggc ggagggaat gtcgaagac gatgaggga tgcgggttt gtgtgtccg ggagaggca gggttgtgt atagctgcg ggacttgtt tgtaatgga gtgattaat tgcccgaat gttaccgat cacaaaggt accaccttt agtgacgaa tctgcggag ttgtgcgtg tcggaacac agccccgag atcaattgg atttacgtg ggggcggcg gcagtgtca gtgttactc ctcctgtgt tgtttttgt gtctgttta tgcaggagg tgttgtaga tactgttgc agcgacgac taa
- >TC033963 RA
tgcgtcggt tcaccgtga gtgcaactt caatggtgt cgaaagctc gattgcgat agtgccaat gatgtaatt ggtgtgagt gtgagcttt tgctgcagc tttgctgtg agttatgtg cttatgatg atttgtgcg cattgagaa aaatgatgg ccgggaggg aagtgctgt atctcgtct tgctggccg ccagcgtct tcataggac acggctatt cgagtccct cgccaatca aaagaactg atccgaaga aagaccact gcaacaaaa cggtggaaa tttacgagg acgtcgcaa gtccggaag tgacgagtg agaacttcg ggaaaccga tgacatgtt ggtacaggt ttcggtcgt tccgcggaa ctcccaaag attggattc ttcggataa agttcaaca aattcaaag tgggtgttt tgctcaacg ctagccact gcgagggag gatatatgc agatcgtcg atggcaacg caaagacgg acgtctcaa accgcaaag agcccggca ttttttgcg gtgagagtg aacaacctc aaactttca tgtcggaaa cgtctttcg tgaaggttg tgtttcata gcgataatt tcaccgacc aaacttact ttagtttcg atacgcgag ccgaacaac aactcgttg tatatttga ggtatggcc agcacccgg aactttatc caaatagac gaggggagg tcgttcagg ggtcttact gcgagagga tattccggg actgccgcc ttcaaactt gttacgtgc aatctccgg cctatcccg gagtctatc cccggaatt tgcactgca gatattacc tgaacacaa gactgcctt tcatcaagc tgtacatcg agaacgaag aattcaaca tcgacggac agcgatgcg aaaatatca tgacatgtc ccatgaggc cgatatcct caggccagg agaattgcc cctacgact acataaaaa tctacgacg gaaagaacg agttgagtc ctgttattg gaacgtttt gcgggatgg gaaagttcc cgtactcca ttataggca catctcagg accttttcg tggagtttg tttcgtcgc cggcaggcc ctttgctca acactggtt tccacttca acgtcggaa actggccag gccacgtgg atacggccg ggagtcgaa acggcacct gtgactggc tcctgtcct ccgagagtc ttcgagcat ctggagatt ccgaaggaa ttttcctct ccgtagccc actggtacc ccccacata ccagctgta catatctca tgcaagggg aagagggcg aaatcgtcc gactatatt tccccagtt ttcggataa accgaatcg agtctccaa tccagccaa ttgaaggcg actgtggcg agtcgctca ctctttacg actcctcct ggcctgacg actcaaaaa tcatcaaga ctttctgtg ataccttct ccaaagcaa tggaaaagc acgatttcg tctcaaccg gaaacgccc tcttcgttc gcttcgaga gcaaaaccg ggagttaca gcggctcgt ctttgtact attgggccc attacgatt ttttcaaca acacgaaat tgggcgaac cagtgcctg gcaaaccct gcgatgaag tgttcgctt cgtggaggc aaactcgag gctggttga ggtctccgc tgaacactt tggtttaca agcagacac agtcgagtg aagatgtgc aatgtttgt accgttttg ttaccgatc ggagacttt tcgctcgtg ttattttga ctataacga acatcgatt tcaaggaga atccataca atcaagcgc cttgctcaa attgtttgg aggatcgag ttgataagt tggtaattt gggagcctc ttcctccgg gggctaaca cctcgtatc cgccgatac cgttgacca aggcggccc aaagtggca atgtgactt gcttgtgta gcaagcaca cttcttcga ctcaaatca tatcaagag gagaacaac ttaatcttc agctgattg tggatagtg cgcattcgg cgttgagct acttcaaac acaattcgc cgctttttg aagccacgt atgagtttg tgcacgggc cgctgtgtg ggcctccaa ttattcaac cacttctaa atggtgaaa tccactacc cttactacg aagccatcg gctacgtgg agcctccca aatcaatcc gctgcatct gggagatta aagtaaacc gagagcgcg acctttggt tgcacttcg accgcatta aattctcct caaaggctt gcgacgacg ggatagtgg acatcttcc tcaaaggtc gtctcgagc catacttat cgatctgcg gcgagaacg tttctctcg tcaaagaac tgccagtgt tgtcagcgg ccgaactaa gtcctgatg gaactgaac cgggagtta caatccagt tcatcggga gaacatcac caacgaggg cagctttca ctatagcgt ggacggagt tgttccatt tgccgagga atcaagacg gaagtctta tgtcttcga gacttacag gcggaggga atgtcgaag acgatgagg gatgcgggt ttgtgtgtc cgggagagg cagggttgt gtatagctg cgggacttg tttgtaatg gagtgatta attgcccga atgttaccg atcacaaag gtaccacct ttagtgacg aatctgcgg agttgtgcg tgtcggaac acagccccg agatcaatt ggatttacg tgggggcgg cggcagtgt cagtgttac tcctcctgt gttgttttt gtgtctgtt tatgcagga ggtgttgta gatactgtt gcagcgacg actaaagtt caattttac gaaaccgaa gccttaatg aaccttttt aacgaaaaa atacaaact aaaagttca agtcttgac tacgtgaca atccaacaa taaattttc cttttgtaa aaaccgcaa tgtagtcca agacttgac cttttaata gagacttaa agcgaagtg aagtgcaca tcgatggaa ctttttggc aaagcactc acgtttagt ttagggtct ttagagtac cgttgtatt atttccaag agagatttg aaccttgca cagttttta cgattcaac aaataaagc gatcatgtt ctaaaattt gtttttgta aaataccta atgccaaaa attcgaaga agtgtatat ttttagcga taagttttt aatttaaat gatgtacat attatgtaa tactttcac atttccatg tgataataa accattgtt ttaaaa
- >TC033963 RB
ccggatccg gagtcgcga ccggcttat tgcaggaat gtttgggtg ttaccatta taaacagat gccggaggc cgactagtt atggaacaa catctgcat cagttcaag gttatagcc attattgtg atggaagct gagacttat atgggatat aatttagtt caagttttg tacgatgtg ggattaatg gatgcgagg aacggctta ctgctgcaa gttcactac agctacaac ttttacatt tttacgccg aaagagcat tcaaatttt aaaccgatg gaatttagc ttaactgat ccgaagaaa gaccactgc aacaaaacg gtggaaatt tacgaggac gtcgcaagt ccggaagtg acgagtgag aacttcggg aaaccgatg acatgttgg tacaggttt cggtcgttc cgcggaact cccaaagat tggattctt cggataaag ttcaacaaa ttcaaagtg ggtgttttg ctcaacgct agccactgc gagggagga tatatgcag atcgtcgat ggcaacgca aagacggac gtctcaaac cgcaaagag cccggcatt ttttgcggt gagagtgaa caacctcaa actttcatg tcggaaacg tctttcgtg aaggttgtg tttcatagc gataatttc accgaccaa acttacttt agtttcgat acgcgagcc gaacaacaa ctcgttgta tatttgagg tatggccag cacccggaa ctttatcca aatagacga ggggaggtc gttcagggg tcttactgc gagaggata ttccgggac tgccgcctt caaacttgt tacgtgcaa tctccggcc tatcccgga gtctatccc cggaatttg cactgcaga tattacctg aacacaaga ctgcctttc atcaagctg tacatcgag aacgaagaa ttcaacatc gacggacag cgatgcgaa aatatcatg acatgtccc atgaggccg atatcctca ggccaggag aattgcccc tacgactac ataaaaatc tacgacgga aagaacgag ttgagtcct gttattgga acgttttgc gggatggga aagttcccg tactccatt ataggcaca tctcaggac cttttcgtg gagtttgtt tcgtcgccg gcaggccct ttgctcaac actggtttc cacttcaac gtcggaaac tggccaggc cacgtggat acggccggg agtcgaaac ggcacctgt gactggctc ctgtcctcc gagagtctt cgagcatct ggagattcc gaaggaatt ttcctctcc gtagcccac tggtacccc ccacatacc agctgtaca tatctcatg caaggggaa gagggcgaa atcgtccga ctatatttc cccagtttt cggataaac cgaatcgag tctccaatc cagccaatt gaaggcgac tgtggcgag tcgctcact ctttacgac tcctcctgg cctgacgac tcaaaaatc atcaagact ttctgtgat accttctcc aaagcaatg gaaaagcac gatttcgtc tcaaccgga aacgccctc ttcgttcgc ttcgagagc aaaaccggg agttacagc ggctcgtct ttgtactat tgggcccat tacgatttt ttcaacaac acgaaattg ggcgaacca gtgcctggc aaaccctgc gatgaagtg ttcgcttcg tggaggcaa actcgaggc tggttgagg tctccgctg aacactttg gtttacaag cagacacag tcgagtgaa gatgtgcaa tgtttgtac cgttttgtt accgatcgg agacttttc gctcgtgtt attttgact ataacgaac atcgatttc aaggagaat ccatacaat caagcgcct tgctcaaat tgtttggag gatcgagtt gataagttg gtaatttgg gagcctctt cctccgggg gctaacacc tcgtatccg ccgataccg ttgaccaag gcggcccaa agtggcaat gtgacttgc ttgtgtagc aagcacact tcttcgact caaatcata tcaagagga gaacaactt aatcttcag ctgattgtg gatagtgcg cattcggcg ttgagctac ttcaaacac aattcgccg ctttttgaa gccacgtat gagtttgtg cacgggccg ctgtgtggg cctccaatt attcaacca cttctaaat ggtgaaatc cactaccct tactacgaa gccatcggc tacgtggag cctcccaaa tcaatccgc tgcatctgg gagattaaa gtaaaccga gagcgcgac ctttggttg cacttcgac cgcattaaa ttctcctca aaggcttgc gacgacggg atagtggac atcttcctc aaaggtcgt ctcgagcca tacttatcg atctgcggc gagaacgtt tctctcgtc aaagaactg ccagtgttg tcagcggcc gaactaagt cctgatgga actgaaccg ggagttaca atccagttc atcgggaga acatcacca acgagggca gctttcact atagcgtgg acggagttg ttccatttg ccgaggaat caagacgga agtcttatg tcttcgaga cttacaggc ggagggaat gtcgaagac gatgaggga tgcgggttt gtgtgtccg ggagaggca gggttgtgt atagctgcg ggacttgtt tgtaatgga gtgattaat tgcccgaat gttaccgat cacaaaggt accaccttt agtgacgaa tctgcggag ttgtgcgtg tcggaacac agccccgag atcaattgg atttacgtg ggggcggcg gcagtgtca gtgttactc ctcctgtgt tgtttttgt gtctgttta tgcaggagg tgttgtaga tactgttgc agcgacgac taaagttca attttacga aaccgaagc cttaatgaa cctttttaa cgaaaaaat acaaactaa aagttcaag tcttgacta cgtgacaat ccaacaata aattttcct tttgtaaaa accgcaatg tagtccaag acttgacct tttaataga gacttaaag cgaagtgaa gtgcacatc gatggaact ttttggcaa agcactcac gtttagttt agggtcttt agagtaccg ttgtattat ttccaagag agatttgaa ccttgcaca gtttttacg attcaacaa ataaagcga tcatgttct aaaatttgt ttttgtaaa atacctaat gccaaaaat tcgaagaag tgtatattt ttagcgata agtttttaa tttaaatga tgtacatat tatgtaata ctttcacat ttccatgtg ataataaac cattgtttt aaaa
