| General information | |||
| TC033954
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 2610694..2618235 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033954 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02890 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
- >TC033954 RA
MNDQIEHEI CGNCKKEIP QHNYVMHSA HCARNITLC PVCKEPIPK SQFESHKKN CVAVKPKPR KPEPPPTNL EKSSYFQER KAVEDKKIA ERKEKQLKK LEKFVDLGH TLKGSEKKA SLIACKFCE LELPKLELE EHENYCGTR TDKCLQCGE LVMFKYKQI HMDSNHGFL KLKDEPGPR PSWDSTTQR SENSYTPRP RPPPLSFSS FDASVYSSY STPSPIKKE KGESYKEIS RRLDCKTEY IRNLLHDSA SITVPLRHN GAVPRNHFN HNKGPAPQP PRRRPNPPT ELTIPCEFC NVPIPHDDL IQHETGCRP DLARFNPRR RVPSEEDDY FVPPQPSSP EVELPCEFC ADMIPASQL LRHQATCI
- >TC033954 RA
atgaacgac caaatcgag cacgaaata tgcggcaat tgcaaaaag gagattcct cagcacaac tacgttatg cacagtgca cactgcgcc cgcaacatc acgctatgt ccagtgtgc aaggaaccg atccccaaa agccagttc gaaagccac aagaaaaac tgcgtggcg gttaagcca aagccgcgg aaacccgag ccgcccccg accaacttg gagaaaagc tcgtacttc caagagcgc aaagcggtc gaagacaag aaaatcgca gagcgcaag gagaaacaa ttgaagaaa ttggagaag tttgtggat ttggggcac acgctgaaa ggcagcgag aagaaagcc agcttgatt gcgtgcaag ttttgcgag cttgagctc ccgaagctg gagctggag gagcatgag aactattgt ggcacgagg accgataag tgtctgcag tgtggggag ctggtcatg ttcaagtat aagcaaatc catatggat tcgaatcac ggatttctc aaactgaaa gatgagcca gggcctcga ccatcatgg gactccacc acccagcgt tcggaaaat tcctacact ccacgtccg agaccacca cctttgagc ttttccagc tttgatgcg tcagtctat tcgagctat tcaacccca tcgccgatt aagaaggaa aaaggggag agctacaag gagatttca agacggctt gattgcaag accgagtac attcgtaat ttgctgcac gattcggcc agcattact gtaccttta cggcacaac ggggctgtc ccgaggaat cacttcaac cacaacaaa ggtccggcc ccccagccc ccaaggcga aggcccaat cctccgaca gaattaacg attccttgc gagttttgc aacgtcccg ataccgcac gatgatctg atccagcac gaaacgggg tgcaggccc gacctggcc agatttaac cccagaaga cgggtgcct tcggaggag gacgactac tttgtgccc cctcaaccc agctcccct gaggtcgag ttgccgtgc gaattttgc gccgatatg ataccggcg tcccaactt ttgcgccac caagccacc tgtatctaa
- >TC033954 RA
cacctcgtg tagagaaaa ggtagacta ttatttttt aaataacgc aaccctacc gcggcaata aattgtccc ttacatttt tttgacaga taagcataa tttatatca gttctaaat acctatagt ttggaaaca acgaaattt ggtttttaa aataaaatg ctcaagctt ttaccgatt aattcccaa tttgaacaa atggacatg gttcggtgt ataactggt tgcctgcca ttcgtgttt tgaattatt cgtttttaa ttaaaataa caaagacga agattcata aagcaacaa gtcgtcccg aaattagat ttgtaaaca ctttttgcg tgtcactta ttgttttca agacagtgg ccaggattt gccaattaa ttgtatccg aaaaattga acttccttt atgttttaa tcggtcaca cgtttgata taattagct tgtccatgt tttaatccg ttgcacgcg tgatgacgc acaatcagc taaacgggg gaagtccct attaaagca gtttgcatc tgcgtcgtt cccctccga taacgtgtg cacttttcc cccacatag ttacgcaaa aaatcgcga tacgaaatt aataatcgt aaaaatagt gaacccatc agtggtggt ttggaataa aatgccttg gtgttaaaa atagtttcg tacagtcat tggaagagt cgtgaaggc taaaaatac gcatttacg acaaccttt agtgttctc ctcagtgca ttcacacgc gttttgcga aaaccaaag tgcggaaaa tgaacgacc aaatcgagc acgaaatat gcggcaatt gcaaaaagg agattcctc agcacaact acgttatgc acagtgcac actgcgccc gcaacatca cgctatgtc cagtgtgca aggaaccga tccccaaaa gccagttcg aaagccaca agaaaaact gcgtggcgg ttaagccaa agccgcgga aacccgagc cgcccccga ccaacttgg agaaaagct cgtacttcc aagagcgca aagcggtcg aagacaaga aaatcgcag agcgcaagg agaaacaat tgaagaaat tggagaagt ttgtggatt tggggcaca cgctgaaag gcagcgaga agaaagcca gcttgattg cgtgcaagt tttgcgagc ttgagctcc cgaagctgg agctggagg agcatgaga actattgtg gcacgagga ccgataagt gtctgcagt gtggggagc tggtcatgt tcaagtata agcaaatcc atatggatt cgaatcacg gatttctca aactgaaag atgagccag ggcctcgac catcatggg actccacca cccagcgtt cggaaaatt cctacactc cacgtccga gaccaccac ctttgagct tttccagct ttgatgcgt cagtctatt cgagctatt caaccccat cgccgatta agaaggaaa aaggggaga gctacaagg agatttcaa gacggcttg attgcaaga ccgagtaca ttcgtaatt tgctgcacg attcggcca gcattactg tacctttac ggcacaacg gggctgtcc cgaggaatc acttcaacc acaacaaag gtccggccc cccagcccc caaggcgaa ggcccaatc ctccgacag aattaacga ttccttgcg agttttgca acgtcccga taccgcacg atgatctga tccagcacg aaacggggt gcaggcccg acctggcca gatttaacc ccagaagac gggtgcctt cggaggagg acgactact ttgtgcccc ctcaaccca gctcccctg aggtcgagt tgccgtgcg aattttgcg ccgatatga taccggcgt cccaacttt tgcgccacc aagccacct gtatctaaa ttttgttgc atcgagaga aatgtttaa tttaatacg ctttctatt ctagcttcg taatgtagt tgttttatt attattaaa taaataaag ttttttacg ccca
