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Gene information
General information
TC033954
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006115
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2610694..2618235
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC033954
      iBeetle screen iB_02890

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection


      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
      Days post injection


      Fertility
      fertility after larval injection decreased (no living offspring) -
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC033954 RA
        MNDQIEHEI CGNCKKEIP QHNYVMHSA HCARNITLC 
        PVCKEPIPK SQFESHKKN CVAVKPKPR KPEPPPTNL 
        EKSSYFQER KAVEDKKIA ERKEKQLKK LEKFVDLGH 
        TLKGSEKKA SLIACKFCE LELPKLELE EHENYCGTR 
        TDKCLQCGE LVMFKYKQI HMDSNHGFL KLKDEPGPR 
        PSWDSTTQR SENSYTPRP RPPPLSFSS FDASVYSSY 
        STPSPIKKE KGESYKEIS RRLDCKTEY IRNLLHDSA 
        SITVPLRHN GAVPRNHFN HNKGPAPQP PRRRPNPPT 
        ELTIPCEFC NVPIPHDDL IQHETGCRP DLARFNPRR 
        RVPSEEDDY FVPPQPSSP EVELPCEFC ADMIPASQL 
        LRHQATCI
      CDS sequence
      • >TC033954 RA
        atgaacgac caaatcgag cacgaaata tgcggcaat 
        tgcaaaaag gagattcct cagcacaac tacgttatg 
        cacagtgca cactgcgcc cgcaacatc acgctatgt 
        ccagtgtgc aaggaaccg atccccaaa agccagttc 
        gaaagccac aagaaaaac tgcgtggcg gttaagcca 
        aagccgcgg aaacccgag ccgcccccg accaacttg 
        gagaaaagc tcgtacttc caagagcgc aaagcggtc 
        gaagacaag aaaatcgca gagcgcaag gagaaacaa 
        ttgaagaaa ttggagaag tttgtggat ttggggcac 
        acgctgaaa ggcagcgag aagaaagcc agcttgatt 
        gcgtgcaag ttttgcgag cttgagctc ccgaagctg 
        gagctggag gagcatgag aactattgt ggcacgagg 
        accgataag tgtctgcag tgtggggag ctggtcatg 
        ttcaagtat aagcaaatc catatggat tcgaatcac 
        ggatttctc aaactgaaa gatgagcca gggcctcga 
        ccatcatgg gactccacc acccagcgt tcggaaaat 
        tcctacact ccacgtccg agaccacca cctttgagc 
        ttttccagc tttgatgcg tcagtctat tcgagctat 
        tcaacccca tcgccgatt aagaaggaa aaaggggag 
        agctacaag gagatttca agacggctt gattgcaag 
        accgagtac attcgtaat ttgctgcac gattcggcc 
        agcattact gtaccttta cggcacaac ggggctgtc 
        ccgaggaat cacttcaac cacaacaaa ggtccggcc 
        ccccagccc ccaaggcga aggcccaat cctccgaca 
        gaattaacg attccttgc gagttttgc aacgtcccg 
        ataccgcac gatgatctg atccagcac gaaacgggg 
        tgcaggccc gacctggcc agatttaac cccagaaga 
        cgggtgcct tcggaggag gacgactac tttgtgccc 
        cctcaaccc agctcccct gaggtcgag ttgccgtgc 
        gaattttgc gccgatatg ataccggcg tcccaactt 
        ttgcgccac caagccacc tgtatctaa
      mRNA sequence
      • >TC033954 RA
        cacctcgtg tagagaaaa ggtagacta ttatttttt 
        aaataacgc aaccctacc gcggcaata aattgtccc 
        ttacatttt tttgacaga taagcataa tttatatca 
        gttctaaat acctatagt ttggaaaca acgaaattt 
        ggtttttaa aataaaatg ctcaagctt ttaccgatt 
        aattcccaa tttgaacaa atggacatg gttcggtgt 
        ataactggt tgcctgcca ttcgtgttt tgaattatt 
        cgtttttaa ttaaaataa caaagacga agattcata 
        aagcaacaa gtcgtcccg aaattagat ttgtaaaca 
        ctttttgcg tgtcactta ttgttttca agacagtgg 
        ccaggattt gccaattaa ttgtatccg aaaaattga 
        acttccttt atgttttaa tcggtcaca cgtttgata 
        taattagct tgtccatgt tttaatccg ttgcacgcg 
        tgatgacgc acaatcagc taaacgggg gaagtccct 
        attaaagca gtttgcatc tgcgtcgtt cccctccga 
        taacgtgtg cacttttcc cccacatag ttacgcaaa 
        aaatcgcga tacgaaatt aataatcgt aaaaatagt 
        gaacccatc agtggtggt ttggaataa aatgccttg 
        gtgttaaaa atagtttcg tacagtcat tggaagagt 
        cgtgaaggc taaaaatac gcatttacg acaaccttt 
        agtgttctc ctcagtgca ttcacacgc gttttgcga 
        aaaccaaag tgcggaaaa tgaacgacc aaatcgagc 
        acgaaatat gcggcaatt gcaaaaagg agattcctc 
        agcacaact acgttatgc acagtgcac actgcgccc 
        gcaacatca cgctatgtc cagtgtgca aggaaccga 
        tccccaaaa gccagttcg aaagccaca agaaaaact 
        gcgtggcgg ttaagccaa agccgcgga aacccgagc 
        cgcccccga ccaacttgg agaaaagct cgtacttcc 
        aagagcgca aagcggtcg aagacaaga aaatcgcag 
        agcgcaagg agaaacaat tgaagaaat tggagaagt 
        ttgtggatt tggggcaca cgctgaaag gcagcgaga 
        agaaagcca gcttgattg cgtgcaagt tttgcgagc 
        ttgagctcc cgaagctgg agctggagg agcatgaga 
        actattgtg gcacgagga ccgataagt gtctgcagt 
        gtggggagc tggtcatgt tcaagtata agcaaatcc 
        atatggatt cgaatcacg gatttctca aactgaaag 
        atgagccag ggcctcgac catcatggg actccacca 
        cccagcgtt cggaaaatt cctacactc cacgtccga 
        gaccaccac ctttgagct tttccagct ttgatgcgt 
        cagtctatt cgagctatt caaccccat cgccgatta 
        agaaggaaa aaggggaga gctacaagg agatttcaa 
        gacggcttg attgcaaga ccgagtaca ttcgtaatt 
        tgctgcacg attcggcca gcattactg tacctttac 
        ggcacaacg gggctgtcc cgaggaatc acttcaacc 
        acaacaaag gtccggccc cccagcccc caaggcgaa 
        ggcccaatc ctccgacag aattaacga ttccttgcg 
        agttttgca acgtcccga taccgcacg atgatctga 
        tccagcacg aaacggggt gcaggcccg acctggcca 
        gatttaacc ccagaagac gggtgcctt cggaggagg 
        acgactact ttgtgcccc ctcaaccca gctcccctg 
        aggtcgagt tgccgtgcg aattttgcg ccgatatga 
        taccggcgt cccaacttt tgcgccacc aagccacct 
        gtatctaaa ttttgttgc atcgagaga aatgtttaa 
        tttaatacg ctttctatt ctagcttcg taatgtagt 
        tgttttatt attattaaa taaataaag ttttttacg 
        ccca
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY