Status
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Gene information
General information
TC002221 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 1106552..1112104
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002221
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02889

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    Pupal morphology
    gnathal appendages partiallyorientation irregular - Number of animals showing the phenotype: 5

    wings orientation irregular - Number of animals showing the phenotype: 5

    elytra orientation irregular - Number of animals showing the phenotype: 5

    hindwing orientation irregular - Number of animals showing the phenotype: 5

    Adult morphology
    flagellomeres number decreased - Number of animals showing the phenotype: 2


    flagellomeres fused - Number of animals showing the phenotype: 2

    elytra surface irregular - Number of animals showing the phenotype: 2

    elytra shortened - Number of animals showing the phenotype: 2


    elytra partially blistered - Number of animals showing the phenotype: 2


    elytra distal colour lighter - Number of animals showing the phenotype: 2

    hindwing partially folding irregular - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002221 RA
      MTETVTDQQ QYEAGEAVL GQSFVSYAN YNFENSALE 
LARCQWSNY ADQHYLKGC KWSPDGTCL LTVVRGAGM 
HVMELPTDL YTGETIMNS RPLVALSPA VSVPESGLI 
YDYCWYPGM NSANPATCC WLSSGHEGP IHLWDAFTG 
NLRCSYRGY NNVDELEAA ISVTFSADG QNVFCGYKK 
NVKIFSTGR PGREFVEYP TTHPASCLV ASQAQPGVV 
AIGTWKNTI ELVAQSDGT FRHLCKLQG HRGGITSMA 
FSLDGFRLY SGARKDKEI ICWDLRVPG RPMFCLTRE 
ANTNQKISI DLSMCGNWL VSGGTDGKV QVWNVHDST 
YPSVHMQMP LHSDSCNGV SLHPYRPIL ATATGQHHP 
LDPLHHTRT AMVYENALC MWWVGQQQP DPDLLASIM 
KAAAKSNS
    • >TC002221 RB
      MTETVTDQQ QYEAGEAVL GQSFVSYAN YNFENSALE 
LARCQWSNY ADQHYLKGC KWSPDGTCL LTVVRGAGM 
HVMELPTDL YTGETIMNS RPLVALSPA VSVPESGLI 
YDYCWYPGM NSANPATCC WLSSGHEGP IHLWDAFTG 
NLRCSYRGY NNVDELEAA ISVTFSADG QNVFCGYKK 
NVKIFSTGR PGREFVEYP TTHPASCLV ASQAQPGVV 
AIGTWKNTI ELVAQSDGT FRHLCKLQG HRGGITSMA 
FSLDGFRLY SGARKDKEI ICWDLRVPG RPMFCLTRE 
ANTNQKISI DLSMCGNWL VSGGTDGKV QVWNVHDST 
YPSVHMQVS TLWNYLLCN YFMFSVSNL VTFLFES
    CDS sequence
    • >TC002221 RA
      atgacggag acagtgacg gatcaacaa caatacgaa 
gcgggcgag gccgtgctc gggcagtcg ttcgtctcg 
tacgccaat tataacttt gagaactca gctttagaa 
ttagcacgg tgtcagtgg tcgaattat gccgatcaa 
cattacctc aaagggtgt aagtggtcg cccgatggg 
acctgtctc cttactgtg gtccgaggg gctgggatg 
catgtcatg gagctgcca actgatctc tacacgggg 
gagacaatc atgaatagc cgacccctt gtggcgctc 
agccccgct gtcagtgtg cctgagagt ggactcatc 
tacgattat tgttggtat ccggggatg aatagcgca 
aatccagca acgtgttgt tggttatca agtggtcat 
gaaggccca atccatttg tgggacgct ttcacagga 
aatttgcgg tgttcatac agagggtac aacaatgtc 
gatgaactg gaagctgca ataagcgta actttttca 
gctgatggt cagaatgta ttttgtggt tacaagaag 
aatgttaaa atttttagt accggtcga ccgggacgg 
gagtttgtc gagtatcca acgacgcat cctgcttcc 
tgtctggtg gctagtcaa gcgcagccc ggcgttgta 
gcaataggt acctggaaa aacaccatc gaattggta 
gctcaaagc gacggtaca ttccgtcac ctgtgcaag 
ttgcaaggc cacagaggg ggcattaca tcaatggct 
ttctcactg gacggcttc cggctgtac agcggcgcg 
cgaaaggat aaagaaatc atctgctgg gatctacga 
gtgccaggt cggccaatg ttctgtttg acacgcgaa 
gcaaatacc aaccaaaaa atcagtatt gacctgtcc 
atgtgtggc aactggctc gtctcaggg ggcacggat 
ggtaaggtg caagtttgg aatgttcac gatagtacc 
tatccaagc gtacacatg cagatgcct ctgcatagt 
gacagttgc aacggcgta agtttacat ccttatcga 
ccgattctt gcaaccgct acaggacag caccatcct 
ctagaccct cttcatcat acaagaact gcaatggtt 
tatgaaaat gcgctgtgt atgtggtgg gtgggccaa 
caacaacca gatcctgat ttgctagct tcgatcatg 
aaagcggct gctaagtct aactcttag
    • >TC002221 RB
      atgacggag acagtgacg gatcaacaa caatacgaa 
gcgggcgag gccgtgctc gggcagtcg ttcgtctcg 
tacgccaat tataacttt gagaactca gctttagaa 
ttagcacgg tgtcagtgg tcgaattat gccgatcaa 
cattacctc aaagggtgt aagtggtcg cccgatggg 
acctgtctc cttactgtg gtccgaggg gctgggatg 
catgtcatg gagctgcca actgatctc tacacgggg 
gagacaatc atgaatagc cgacccctt gtggcgctc 
agccccgct gtcagtgtg cctgagagt ggactcatc 
tacgattat tgttggtat ccggggatg aatagcgca 
aatccagca acgtgttgt tggttatca agtggtcat 
gaaggccca atccatttg tgggacgct ttcacagga 
aatttgcgg tgttcatac agagggtac aacaatgtc 
gatgaactg gaagctgca ataagcgta actttttca 
gctgatggt cagaatgta ttttgtggt tacaagaag 
aatgttaaa atttttagt accggtcga ccgggacgg 
gagtttgtc gagtatcca acgacgcat cctgcttcc 
tgtctggtg gctagtcaa gcgcagccc ggcgttgta 
gcaataggt acctggaaa aacaccatc gaattggta 
gctcaaagc gacggtaca ttccgtcac ctgtgcaag 
ttgcaaggc cacagaggg ggcattaca tcaatggct 
ttctcactg gacggcttc cggctgtac agcggcgcg 
cgaaaggat aaagaaatc atctgctgg gatctacga 
gtgccaggt cggccaatg ttctgtttg acacgcgaa 
gcaaatacc aaccaaaaa atcagtatt gacctgtcc 
atgtgtggc aactggctc gtctcaggg ggcacggat 
ggtaaggtg caagtttgg aatgttcac gatagtacc 
tatccaagc gtacacatg caggtgagt accctttgg 
aattacctc ttatgtaat tatttcatg ttttcagta 
tctaattta gtcacgttt ctatttgaa tcataa
    mRNA sequence
    • >TC002221 RA
      cggtgtata aaagtaaat ttaaatctc ccgccacga 
tgttggtgt tttttgcgt cgcggctga tttcggttg 
gcgacttgc gatcgttta tgtcatttt ctgtagaca 
cattccgac gttgttgat ccattttcg gtgtatttg 
tggcaattt tgtcgtgtt gactgtaaa aaagccgtg 
aatgtgttt agtttttag gtgtcgtta ataaaaatg 
acggagaca gtgacggat caacaacaa tacgaagcg 
ggcgaggcc gtgctcggg cagtcgttc gtctcgtac 
gccaattat aactttgag aactcagct ttagaatta 
gcacggtgt cagtggtcg aattatgcc gatcaacat 
tacctcaaa gggtgtaag tggtcgccc gatgggacc 
tgtctcctt actgtggtc cgaggggct gggatgcat 
gtcatggag ctgccaact gatctctac acgggggag 
acaatcatg aatagccga ccccttgtg gcgctcagc 
cccgctgtc agtgtgcct gagagtgga ctcatctac 
gattattgt tggtatccg gggatgaat agcgcaaat 
ccagcaacg tgttgttgg ttatcaagt ggtcatgaa 
ggcccaatc catttgtgg gacgctttc acaggaaat 
ttgcggtgt tcatacaga gggtacaac aatgtcgat 
gaactggaa gctgcaata agcgtaact ttttcagct 
gatggtcag aatgtattt tgtggttac aagaagaat 
gttaaaatt tttagtacc ggtcgaccg ggacgggag 
tttgtcgag tatccaacg acgcatcct gcttcctgt 
ctggtggct agtcaagcg cagcccggc gttgtagca 
ataggtacc tggaaaaac accatcgaa ttggtagct 
caaagcgac ggtacattc cgtcacctg tgcaagttg 
caaggccac agagggggc attacatca atggctttc 
tcactggac ggcttccgg ctgtacagc ggcgcgcga 
aaggataaa gaaatcatc tgctgggat ctacgagtg 
ccaggtcgg ccaatgttc tgtttgaca cgcgaagca 
aataccaac caaaaaatc agtattgac ctgtccatg 
tgtggcaac tggctcgtc tcagggggc acggatggt 
aaggtgcaa gtttggaat gttcacgat agtacctat 
ccaagcgta cacatgcag atgcctctg catagtgac 
agttgcaac ggcgtaagt ttacatcct tatcgaccg 
attcttgca accgctaca ggacagcac catcctcta 
gaccctctt catcataca agaactgca atggtttat 
gaaaatgcg ctgtgtatg tggtgggtg ggccaacaa 
caaccagat cctgatttg ctagcttcg atcatgaaa 
gcggctgct aagtctaac tcttaggtt ttttgcatt 
tacgttagt ggggtgcca catcacatt ctagatgat 
aatattgtg cattaaata aaaagtatt ttcacac
    • >TC002221 RB
      cggtgtata aaagtaaat ttaaatctc ccgccacga 
tgttggtgt tttttgcgt cgcggctga tttcggttg 
gcgacttgc gatcgttta tgtcatttt ctgtagaca 
cattccgac gttgttgat ccattttcg gtgtatttg 
tggcaattt tgtcgtgtt gactgtaaa aaagccgtg 
aatgtgttt agtttttag gtgtcgtta ataaaaatg 
acggagaca gtgacggat caacaacaa tacgaagcg 
ggcgaggcc gtgctcggg cagtcgttc gtctcgtac 
gccaattat aactttgag aactcagct ttagaatta 
gcacggtgt cagtggtcg aattatgcc gatcaacat 
tacctcaaa gggtgtaag tggtcgccc gatgggacc 
tgtctcctt actgtggtc cgaggggct gggatgcat 
gtcatggag ctgccaact gatctctac acgggggag 
acaatcatg aatagccga ccccttgtg gcgctcagc 
cccgctgtc agtgtgcct gagagtgga ctcatctac 
gattattgt tggtatccg gggatgaat agcgcaaat 
ccagcaacg tgttgttgg ttatcaagt ggtcatgaa 
ggcccaatc catttgtgg gacgctttc acaggaaat 
ttgcggtgt tcatacaga gggtacaac aatgtcgat 
gaactggaa gctgcaata agcgtaact ttttcagct 
gatggtcag aatgtattt tgtggttac aagaagaat 
gttaaaatt tttagtacc ggtcgaccg ggacgggag 
tttgtcgag tatccaacg acgcatcct gcttcctgt 
ctggtggct agtcaagcg cagcccggc gttgtagca 
ataggtacc tggaaaaac accatcgaa ttggtagct 
caaagcgac ggtacattc cgtcacctg tgcaagttg 
caaggccac agagggggc attacatca atggctttc 
tcactggac ggcttccgg ctgtacagc ggcgcgcga 
aaggataaa gaaatcatc tgctgggat ctacgagtg 
ccaggtcgg ccaatgttc tgtttgaca cgcgaagca 
aataccaac caaaaaatc agtattgac ctgtccatg 
tgtggcaac tggctcgtc tcagggggc acggatggt 
aaggtgcaa gtttggaat gttcacgat agtacctat 
ccaagcgta cacatgcag gtgagtacc ctttggaat 
tacctctta tgtaattat ttcatgttt tcagtatct 
aatttagtc acgtttcta tttgaatca taatttaaa 
aaaaatcat tttgtcttt atttgttgt tacaccaat 
acttacagg ttcccagga ctctatact ttgtctcat 
tttgtttca ctctttatt ttgtttgtt ttctttcaa 
ttttatgta aaataaata aagtgaggt ccttaggaa 
cagtgacaa attcaactt ttggtaaca acgaagact 
ttgtaagag gttataatt aattgggga tcaacgctt 
tcagatgcc tctgcatag tgacagttg caacggcgt 
aagtttaca tccttatcg accgattct tgcaaccgc 
tacaggaca gcaccatcc tctagaccc tcttcatca 
tacaagaac tgcaatggt ttatgaaaa tgcgctgtg 
tatgtggtg ggtgggcca acaacaacc agatcctga 
tttgctagc ttcgatcat gaaagcggc tgctaagtc 
taactctta ggttttttg catttacgt tagtggggt 
gccacatca cattctaga tgataatat tgtgcatta 
aataaaaag tattttcac ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY