Status
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Gene information
General information
TC031292
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006799,TC006798,TC006796,TC006797
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid58: 1179914..1187749
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031292
    iBeetle screen iB_02887
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031292 RA
      METQTEEYT DGLIPPDAF PGGKALSGC DTYIGQAYI 
      HGQGLYIVQ IFPGKKQVD AAWNGVKKA TNDIKILCT 
      ENPENFEWM PTSSQLYHF NTTRVQPVP GGYDFVNND 
      GLFHVGRLA YQGVVKIGG IWSSQIEKA DFYFGHDNA 
      LKKATLYEA LVYRKVVYD IDIIVASFN HFVANTAMK 
      YPLLVSFFL LLLRLISVH CDIGYYWRD FSNSKIPKD 
      AFPAGKDLN GKNTYIGQT LLHTFGFFV MHIIPFSKQ 
      NTVGFYSVV KPSPVQKIL CSKNHDKFS WYPTSAKTF 
      HLDTTEKHA VIGGYDHVD ASRGMLHVG RVMFDGSIK 
      IGSVKGYAL QNAELLFSS NNVIKTASS YEVLLFDNS 
      GIANDRKNV TNYLMINSW RLVVVFFIQ ANAFDEKGY 
      YWKEYTGVI PPDALPGGK DSNDEDLFI GQAYIHNHG 
      LYVVQICPG TMTVNAGWW GVKKATNDI KILCAKKPE 
      NFEWVPSSQ TTFHKDMVN KTPVPGGYD FINNDGTMH 
      IGRIRYQEF VKVGGIWSA KKDNSWLYF DYNNVLKRI 
      SILNKTMGK VKFTEPLEF LNVVGLNPE NCSNFSLFR 
      RVISLGFFL VVITLGLLE LLLHFEGLE TCSRASEAM 
      IVQYQLFIK IAVLLKHRK NLVVLMQKT RKFWPLDKF 
      GQDAKIERP HKLLKAFFF AYKLIMILM ALQYILRKF 
      VSKNGKPLA IAFGESKGL SPKVDHLYF VLHSTSTFV 
      VLHAVTGFD RLFFFLIGH VLTELKLVK KSYRLTQNR 
      REKFLETVQ HHAFALEFV RKLNRIYSQ VLLNQHLSC 
      LFGICFGLF LVSKDGIPP DLGHVTKYV PYVISFITQ 
      TFTFCFIGS LLITWSLQV PDAIFYNDW GKNQAYKYK 
      TDKIIAMIR GQRAAKLTL GGRVGTGGY IMDKVKLAQ 
      PLAHLNIIG LDPLKNDRF SKIRTVITV AVFALCNVF 
      SFSELFLHY NNPHVIVRS SEVVFPFFQ NDWKIAIML 
      VYKKNLAQL IQNTSRFWQ IDAFGKNYQ YSMGIKHKY 
      VRIFYLVYR LMLMFSCSQ YILLTIGSD RPMILSFGE 
      TGGLGSGAL LFYLIFHIV YLLIIFNVI NGFDGLFFF 
      LVAHVLSEL QMVKVAFSS SKVITFWNH KRRFKSAIQ 
      HHRFVLDYI NRLNSIYSI LLLNQHISC LFGICFGLY 
      LFISDGFPP DYEHISKYV PYVIYYITQ VWVFCFAGQ 
      LIIDWSVNI SDEIFYHDW TLNRTYENK TDKLIIIQR 
      AQHAARLSL AGYGNLDLQ SFNLVLKNG LSFFTFVNA 
      VIHK
    CDS sequence
    • >TC031292 RA
      atggaaaca cagacagag gaatacacc gatgggtta 
      atcccacct gacgctttt cctggtgga aaggcctta 
      agtggttgt gacacctac attggacaa gcatacatc 
      cacggacaa ggcctttac attgtgcaa atttttccc 
      ggaaaaaag caagtcgac gctgcttgg aacggcgta 
      aaaaaggcc accaacgat attaaaatc ttgtgtacc 
      gaaaatcct gaaaatttt gagtggatg ccaacgtcc 
      agtcagttg taccatttt aataccacg agggtacaa 
      ccagtacca ggagggtac gattttgtg aacaacgat 
      gggcttttc catgttggt agattggcg tatcaagga 
      gtcgtaaaa attgggggc atttggtcg tcacaaatt 
      gaaaaggcc gatttttat tttgggcat gacaatgcc 
      ctcaagaaa gccactttg tacgaagcg ctagtctat 
      cgcaaagtg gtttatgat atcgatata attgtcgct 
      tcatttaat cattttgtt gcaaacaca gctatgaaa 
      tacccactc ttggtttcc ttcttcttg ttactgcta 
      cgtttgatt tcagttcat tgtgacata ggttactat 
      tggagggac ttttcaaac tcaaaaatc cccaaagat 
      gccttccct gctgggaaa gacctgaac gggaaaaac 
      acctacatt ggacaaacc cttttacac acttttggc 
      tttttcgta atgcacatt attccattt agtaaacaa 
      aatactgtt ggtttttac agtgtggta aaaccaagc 
      ccagtccaa aagatttta tgttcaaaa aatcacgac 
      aaattttcg tggtatccg acaagtgcc aaaactttt 
      cacttggac actacagaa aaacatgca gtgataggg 
      ggttacgat catgttgat gcttctaga gggatgtta 
      catgtaggt cgtgtgatg tttgatgga agtataaaa 
      attgggagt gttaaagga tacgcattg caaaatgcg 
      gaactttta ttcagttca aataacgtt ataaaaaca 
      gcctcatct tacgaagtg ttgctgttt gacaacagt 
      ggtattgcc aatgatagg aaaaatgta accaactat 
      ttgatgatc aactcgtgg cgtcttgtt gttgtattt 
      ttcattcaa gcaaatgcg tttgatgaa aaaggctac 
      tactggaag gaatacacc ggtgttatt cctccagac 
      gcccttcca ggcggcaaa gactcaaac gatgaggac 
      ctatttatc ggacaagct tacatccac aaccatggt 
      ctttacgtt gtgcaaatt tgtcccgga acaatgacc 
      gtcaatgct ggttggtgg ggtgtcaag aaagcaaca 
      aacgatatt aaaattttg tgtgccaaa aaaccggaa 
      aattttgaa tgggtgccg tcgtcccaa acaacgttt 
      cataaagac atggtcaac aaaacccca gtgcctgga 
      ggttacgat tttatcaac aacgatgga actatgcac 
      attgggcgt ataaggtac caggagttt gtgaaagtt 
      ggagggatc tggtcggcg aaaaaagac aactcttgg 
      ctgtacttc gattacaac aatgtttta aaacgaatt 
      tcgattttg aacaaaacc atgggcaaa gtcaagttt 
      acggaacct ttggagttt ctaaacgtt gtgggtttg 
      aacccggag aactgctca aacttttcg ctgtttcgg 
      agagtcatt tcgttgggt tttttcctg gttgtgatc 
      acccttgga ttattggaa ctgttgcta cattttgaa 
      ggccttgag acctgttct cgggcctct gaggcaatg 
      atcgtccaa tatcaactg ttcataaag attgctgtt 
      ttgctcaaa caccgaaaa aatctggtg gttttgatg 
      caaaagact cgcaagttt tggccgctt gataagttc 
      ggacaagac gcaaaaatc gaacgtcca cataaatta 
      ctgaaagcg tttttcttc gcctacaag ttgataatg 
      attctaatg gccttgcag tacatttta cgtaaattt 
      gtctcgaaa aatggcaag cctttggct atagcgttt 
      ggggaaagt aaggggttg tccccgaaa gttgatcat 
      ttgtatttt gtgcttcac tcaacttcg acgtttgtg 
      gttttgcac gctgttact ggttttgac agacttttc 
      ttttttcta attgggcac gtcttgact gaactgaaa 
      ctggtaaaa aaatcgtac agattgaca caaaaccgc 
      agggaaaaa tttctggaa actgtacaa caccatgcc 
      tttgcgtta gaatttgta agaaaatta aaccgaatt 
      tattcccaa gtactgctg aatcagcat ttgagttgt 
      ctttttggc atctgtttt gggctattt ttggtttca 
      aaagatggg ataccacct gacttggga catgttact 
      aaatacgtg ccctatgta atttcattc ataacacaa 
      actttcacg ttttgtttt atcggttct ttgttaatc 
      acctggtct ttgcaagtc cctgatgca attttctac 
      aatgattgg ggcaaaaat caagcttat aaatacaag 
      acagataag attattgca atgattcgg ggccaaaga 
      gctgcgaaa ttaacactt ggaggaaga gtcgggact 
      gggggttac attatggat aaagtcaag ctagcgcag 
      cctctggcc cacctcaac ataatcggt ctagacccg 
      ttgaaaaat gaccgattt tccaaaatt cggaccgtt 
      atcacggtt gcggttttc gccctgtgt aacgttttc 
      tccttctcg gagttattt cttcactac aacaacccg 
      cacgtgatt gtgcgaagc tcagaagtt gtgtttccg 
      tttttccaa aacgattgg aagattgca attatgcta 
      gtatacaaa aaaaatcta gcacaactg attcaaaac 
      acctccagg ttttggcaa atcgatgcg tttggcaaa 
      aattaccaa tacagtatg ggcataaaa cacaaatat 
      gtgcgaata ttttatttg gtgtaccga ttaatgctg 
      atgtttagt tgttcgcaa tatattttg ctgacgatt 
      gggagcgac agaccgatg attttgagt tttggggaa 
      actggaggt cttggttcg ggagctttg ttgttttat 
      ttgatattt catattgtt tatctttta attattttt 
      aacgtaatt aatggtttt gacggtttg ttcttcttt 
      cttgtggct catgtttta tcggagctg cagatggtc 
      aaagttgcg ttcagtagc tcgaaagtg atcacgttt 
      tggaatcac aaaaggagg ttcaagtcg gccattcaa 
      caccataga tttgtgtta gattacatc aatcggctt 
      aactcaatt tattcgata ttgctactg aaccagcac 
      atttcgtgc ctgtttggg atttgtttc ggtttatat 
      cttttcata tcagacggt tttccgcca gactacgaa 
      cacatttcg aaatatgtt ccctatgtt atttattac 
      attacccaa gtttgggtc ttttgtttc gcaggccag 
      ttaataatt gattggagt gttaacatt tccgatgaa 
      atattttat cacgactgg actttaaac agaacctac 
      gaaaataaa accgataaa ttgattatt atccaaagg 
      gcacaacat gctgcgaga ttatcttta gcaggatac 
      ggaaatcta gatctacaa agcttcaat ttggttttg 
      aaaaatggg ctgtcgttt ttcacgttt gtgaatgct 
      gttattcac aagtag
    mRNA sequence
    • >TC031292 RA
      tttttgctc aattttcgt tttattgcc acattatgg 
      aaacacaga cagaggaat acaccgatg ggttaatcc 
      cacctgacg cttttcctg gtggaaagg ccttaagtg 
      gttgtgaca cctacattg gacaagcat acatccacg 
      gacaaggcc tttacattg tgcaaattt ttcccggaa 
      aaaagcaag tcgacgctg cttggaacg gcgtaaaaa 
      aggccacca acgatatta aaatcttgt gtaccgaaa 
      atcctgaaa attttgagt ggatgccaa cgtccagtc 
      agttgtacc attttaata ccacgaggg tacaaccag 
      taccaggag ggtacgatt ttgtgaaca acgatgggc 
      ttttccatg ttggtagat tggcgtatc aaggagtcg 
      taaaaattg ggggcattt ggtcgtcac aaattgaaa 
      aggccgatt tttattttg ggcatgaca atgccctca 
      agaaagcca ctttgtacg aagcgctag tctatcgca 
      aagtggttt atgatatcg atataattg tcgcttcat 
      ttaatcatt ttgttgcaa acacagcta tgaaatacc 
      cactcttgg tttccttct tcttgttac tgctacgtt 
      tgatttcag ttcattgtg acataggtt actattgga 
      gggactttt caaactcaa aaatcccca aagatgcct 
      tccctgctg ggaaagacc tgaacggga aaaacacct 
      acattggac aaacccttt tacacactt ttggctttt 
      tcgtaatgc acattattc catttagta aacaaaata 
      ctgttggtt tttacagtg tggtaaaac caagcccag 
      tccaaaaga ttttatgtt caaaaaatc acgacaaat 
      tttcgtggt atccgacaa gtgccaaaa cttttcact 
      tggacacta cagaaaaac atgcagtga tagggggtt 
      acgatcatg ttgatgctt ctagaggga tgttacatg 
      taggtcgtg tgatgtttg atggaagta taaaaattg 
      ggagtgtta aaggatacg cattgcaaa atgcggaac 
      ttttattca gttcaaata acgttataa aaacagcct 
      catcttacg aagtgttgc tgtttgaca acagtggta 
      ttgccaatg ataggaaaa atgtaacca actatttga 
      tgatcaact cgtggcgtc ttgttgttg tatttttca 
      ttcaagcaa atgcgtttg atgaaaaag gctactact 
      ggaaggaat acaccggtg ttattcctc cagacgccc 
      ttccaggcg gcaaagact caaacgatg aggacctat 
      ttatcggac aagcttaca tccacaacc atggtcttt 
      acgttgtgc aaatttgtc ccggaacaa tgaccgtca 
      atgctggtt ggtggggtg tcaagaaag caacaaacg 
      atattaaaa ttttgtgtg ccaaaaaac cggaaaatt 
      ttgaatggg tgccgtcgt cccaaacaa cgtttcata 
      aagacatgg tcaacaaaa ccccagtgc ctggaggtt 
      acgatttta tcaacaacg atggaacta tgcacattg 
      ggcgtataa ggtaccagg agtttgtga aagttggag 
      ggatctggt cggcgaaaa aagacaact cttggctgt 
      acttcgatt acaacaatg ttttaaaac gaatttcga 
      ttttgaaca aaaccatgg gcaaagtca agtttacgg 
      aacctttgg agtttctaa acgttgtgg gtttgaacc 
      cggagaact gctcaaact tttcgctgt ttcggagag 
      tcatttcgt tgggttttt tcctggttg tgatcaccc 
      ttggattat tggaactgt tgctacatt ttgaaggcc 
      ttgagacct gttctcggg cctctgagg caatgatcg 
      tccaatatc aactgttca taaagattg ctgttttgc 
      tcaaacacc gaaaaaatc tggtggttt tgatgcaaa 
      agactcgca agttttggc cgcttgata agttcggac 
      aagacgcaa aaatcgaac gtccacata aattactga 
      aagcgtttt tcttcgcct acaagttga taatgattc 
      taatggcct tgcagtaca ttttacgta aatttgtct 
      cgaaaaatg gcaagcctt tggctatag cgtttgggg 
      aaagtaagg ggttgtccc cgaaagttg atcatttgt 
      attttgtgc ttcactcaa cttcgacgt ttgtggttt 
      tgcacgctg ttactggtt ttgacagac ttttctttt 
      ttctaattg ggcacgtct tgactgaac tgaaactgg 
      taaaaaaat cgtacagat tgacacaaa accgcaggg 
      aaaaatttc tggaaactg tacaacacc atgcctttg 
      cgttagaat ttgtaagaa aattaaacc gaatttatt 
      cccaagtac tgctgaatc agcatttga gttgtcttt 
      ttggcatct gttttgggc tatttttgg tttcaaaag 
      atgggatac cacctgact tgggacatg ttactaaat 
      acgtgccct atgtaattt cattcataa cacaaactt 
      tcacgtttt gttttatcg gttctttgt taatcacct 
      ggtctttgc aagtccctg atgcaattt tctacaatg 
      attggggca aaaatcaag cttataaat acaagacag 
      ataagatta ttgcaatga ttcggggcc aaagagctg 
      cgaaattaa cacttggag gaagagtcg ggactgggg 
      gttacatta tggataaag tcaagctag cgcagcctc 
      tggcccacc tcaacataa tcggtctag acccgttga 
      aaaatgacc gattttcca aaattcgga ccgttatca 
      cggttgcgg ttttcgccc tgtgtaacg ttttctcct 
      tctcggagt tatttcttc actacaaca acccgcacg 
      tgattgtgc gaagctcag aagttgtgt ttccgtttt 
      tccaaaacg attggaaga ttgcaatta tgctagtat 
      acaaaaaaa atctagcac aactgattc aaaacacct 
      ccaggtttt ggcaaatcg atgcgtttg gcaaaaatt 
      accaataca gtatgggca taaaacaca aatatgtgc 
      gaatatttt atttggtgt accgattaa tgctgatgt 
      ttagttgtt cgcaatata ttttgctga cgattggga 
      gcgacagac cgatgattt tgagttttg gggaaactg 
      gaggtcttg gttcgggag ctttgttgt tttatttga 
      tatttcata ttgtttatc ttttaatta tttttaacg 
      taattaatg gttttgacg gtttgttct tctttcttg 
      tggctcatg ttttatcgg agctgcaga tggtcaaag 
      ttgcgttca gtagctcga aagtgatca cgttttgga 
      atcacaaaa ggaggttca agtcggcca ttcaacacc 
      atagatttg tgttagatt acatcaatc ggcttaact 
      caatttatt cgatattgc tactgaacc agcacattt 
      cgtgcctgt ttgggattt gtttcggtt tatatcttt 
      tcatatcag acggttttc cgccagact acgaacaca 
      tttcgaaat atgttccct atgttattt attacatta 
      cccaagttt gggtctttt gtttcgcag gccagttaa 
      taattgatt ggagtgtta acatttccg atgaaatat 
      tttatcacg actggactt taaacagaa cctacgaaa 
      ataaaaccg ataaattga ttattatcc aaagggcac 
      aacatgctg cgagattat ctttagcag gatacggaa 
      atctagatc tacaaagct tcaatttgg ttttgaaaa 
      atgggctgt cgtttttca cgtttgtga atgctgtta 
      ttcacaagt agataagaa tttcagcat gtaccgaaa 
      attaaagca tacttcacg ta