| General information | |||
| TC006787 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 14255929..14260835 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006787 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02886 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
- >TC006787 RA
MLFGAPKFF FGTGVLVFA LIFVVKSDE SCDIEIENT LKTLLTPQD YPSMQQQAL RKDLLRRFQ QALDRADDE DEMNYKRSI SSLAQWGNL PGKRNLEAL ARAGYIRTL PNPDEEDPN NKRSLSTLA KNDQLPTTF QNNESKRGI ESLARNGEL HNRRDIQEL LDELYNKRN VGSLARNFN FPSYGKRYL ASLVRNGEL KYSGKRNIA SIKAQYPGT RSKRQASYY DGEGGEFSL PVYQNQNVD DYEELVKAL AAAYPNNDK RFLGRLPQM GKPKTTPSP KTRFH
- >TC006787 RB
MLFGAPKFF FGTGVLVFA LIFVVKSDE SCDIEIENT LKTLLTPQD YPSMQQQAL RKDLLRRFQ QALDRADDE DEMNYKRSI SSLAQWGNL PGKRNLEAL ARAGYIRTL PNPDEEDPN NKRSLSTLA KNDQLPTTF QNNESKRGI ESLARNGEL HNRRDIQEL LDELYNKRN VGSLARNFN FPSYGKRYL ASLVRNGEL NGKRNIASI KAQYPGTRS KRQASYYDG EGGEFSLPV YQNQNVDDY EELVKALAA AYPNNDKRF LGRLPQMGK PKTTPSPKT RFH
- >TC006787 RC
MLFGAPKFF FGTGVLVFA LIFVVKSDE SCDIEIENT LKTLLTPQD YPSMQQQAL RKDLLRRFQ QALDRADDE DEMNYKRSI SSLAQWGNL PGKRNLEAL ARAGYIRTL PNPDEEDPN NKRSLSTLA KNDQLPTTF QNNESKRGI ESLARNGEL HNRRDIQEL LDELYNKRN VGSLARNFN FPSYGKRYL ASLVRNGEL KYSGKRNIA SIKAQYPGT RSKRQASYY DGEGGEFSL PVYQNQNVD DYEELVKAL AAAYPNNDK RFLGSVARS GWFRPNSRY RSPEKRHIG ALARLGWLP TLRNVRRFN RSGRSTTLE GCREAATDG QTEDDAFAE DQISLEDKR FLLQPAVDQ ILLRKIFMH PRTHPAIN
- >TC006787 RA
atgcttttc ggtgcgccg aagtttttt tttgggacc ggtgtattg gtgtttgcc ttaattttt gtggtaaaa tcggacgaa tcatgtgat atagaaata gaaaacact ctaaaaacc ttactaaca ccacaagac tacccatcg atgcagcag caggcgctt cgcaaagac ttgttgcgt agatttcaa caggcgctc gatcgagcc gacgacgaa gacgaaatg aattacaaa aggtcgata agttcgcta gcacaatgg ggtaattta cccggaaaa cgtaacttg gaagcactg gccagagcc ggctatatc cgcactcta cccaatccc gacgaggaa gatcccaac aataaaaga agtctctcg actttggcc aaaaacgac caattaccc accacgttc caaaataac gaaagcaaa cggggaatc gagtcgttg gcacgaaac ggcgaatta cacaacagg agagacatc caagaactg ttggacgaa ctctacaac aagcggaac gttggcagt ctggcccgg aattttaat ttccccagt tatggcaaa cgatatttg gcgagtttg gtgcgaaac ggcgaactc aagtacagc gggaaaagg aacatcgcc tcgatcaaa gcccaatat cccggcact aggagtaaa agacaggcg agttattac gacggggag ggtggcgag ttctccctc ccggtgtat caaaaccag aacgtggac gattatgag gagctcgtg aaagcgttg gcggcggct tatcctaat aacgacaag cgattccta gggaggctg ccacagatg ggcaaaccg aagacgacg ccttcgccg aagaccaga tttcactag
- >TC006787 RB
atgcttttc ggtgcgccg aagtttttt tttgggacc ggtgtattg gtgtttgcc ttaattttt gtggtaaaa tcggacgaa tcatgtgat atagaaata gaaaacact ctaaaaacc ttactaaca ccacaagac tacccatcg atgcagcag caggcgctt cgcaaagac ttgttgcgt agatttcaa caggcgctc gatcgagcc gacgacgaa gacgaaatg aattacaaa aggtcgata agttcgcta gcacaatgg ggtaattta cccggaaaa cgtaacttg gaagcactg gccagagcc ggctatatc cgcactcta cccaatccc gacgaggaa gatcccaac aataaaaga agtctctcg actttggcc aaaaacgac caattaccc accacgttc caaaataac gaaagcaaa cggggaatc gagtcgttg gcacgaaac ggcgaatta cacaacagg agagacatc caagaactg ttggacgaa ctctacaac aagcggaac gttggcagt ctggcccgg aattttaat ttccccagt tatggcaaa cgatatttg gcgagtttg gtgcgaaac ggcgaactc aacgggaaa aggaacatc gcctcgatc aaagcccaa tatcccggc actaggagt aaaagacag gcgagttat tacgacggg gagggtggc gagttctcc ctcccggtg tatcaaaac cagaacgtg gacgattat gaggagctc gtgaaagcg ttggcggcg gcttatcct aataacgac aagcgattc ctagggagg ctgccacag atgggcaaa ccgaagacg acgccttcg ccgaagacc agatttcac tag
- >TC006787 RC
atgcttttc ggtgcgccg aagtttttt tttgggacc ggtgtattg gtgtttgcc ttaattttt gtggtaaaa tcggacgaa tcatgtgat atagaaata gaaaacact ctaaaaacc ttactaaca ccacaagac tacccatcg atgcagcag caggcgctt cgcaaagac ttgttgcgt agatttcaa caggcgctc gatcgagcc gacgacgaa gacgaaatg aattacaaa aggtcgata agttcgcta gcacaatgg ggtaattta cccggaaaa cgtaacttg gaagcactg gccagagcc ggctatatc cgcactcta cccaatccc gacgaggaa gatcccaac aataaaaga agtctctcg actttggcc aaaaacgac caattaccc accacgttc caaaataac gaaagcaaa cggggaatc gagtcgttg gcacgaaac ggcgaatta cacaacagg agagacatc caagaactg ttggacgaa ctctacaac aagcggaac gttggcagt ctggcccgg aattttaat ttccccagt tatggcaaa cgatatttg gcgagtttg gtgcgaaac ggcgaactc aagtacagc gggaaaagg aacatcgcc tcgatcaaa gcccaatat cccggcact aggagtaaa agacaggcg agttattac gacggggag ggtggcgag ttctccctc ccggtgtat caaaaccag aacgtggac gattatgag gagctcgtg aaagcgttg gcggcggct tatcctaat aacgacaag cgattccta ggctcggtg gctaggagc ggctggttt cggccaaat tcccgctac cggtcgcct gaaaagcgc cacattggc gctttggca cgtcttggg tggctgccg acgctgcga aacgtccgg agattcaac cgctccggc aggtccacc actttggag ggctgcagg gaggctgcc acagatggg caaaccgaa gacgacgcc ttcgccgaa gaccagatt tcactagag gataagagg tttctactg caacctgct gtcgatcaa atattgtta aggaagata tttatgcat ccgaggaca catccggcg attaattag
- >TC006787 RA
tttcccaaa ctgtataaa accgacgtc cggcgtcgc gacgactgt gagcgtcgg tcgacggtt gatgaggac gtgttgcat ctgaagcaa cagttttgt gtgtctgac gccgctatt ttttcaaat catgctttt cggtgcgcc gaagttttt ttttgggac cggtgtatt ggtgtttgc cttaatttt tgtggtaaa atcggacga atcatgtga tatagaaat agaaaacac tctaaaaac cttactaac accacaaga ctacccatc gatgcagca gcaggcgct tcgcaaaga cttgttgcg tagatttca acaggcgct cgatcgagc cgacgacga agacgaaat gaattacaa aaggtcgat aagttcgct agcacaatg gggtaattt acccggaaa acgtaactt ggaagcact ggccagagc cggctatat ccgcactct acccaatcc cgacgagga agatcccaa caataaaag aagtctctc gactttggc caaaaacga ccaattacc caccacgtt ccaaaataa cgaaagcaa acggggaat cgagtcgtt ggcacgaaa cggcgaatt acacaacag gagagacat ccaagaact gttggacga actctacaa caagcggaa cgttggcag tctggcccg gaattttaa tttccccag ttatggcaa acgatattt ggcgagttt ggtgcgaaa cggcgaact caagtacag cgggaaaag gaacatcgc ctcgatcaa agcccaata tcccggcac taggagtaa aagacaggc gagttatta cgacgggga gggtggcga gttctccct cccggtgta tcaaaacca gaacgtgga cgattatga ggagctcgt gaaagcgtt ggcggcggc ttatcctaa taacgacaa gcgattcct agggaggct gccacagat gggcaaacc gaagacgac gccttcgcc gaagaccag atttcacta gaggataag aggtttcta ctgcaacct gctgtcgat caaatattg ttaaggaag atatttatg catccgagg acacatccg gcgattaat tagtttggt tttttggca catatctcg ataattttt gcatatctc cgatatatc agactcact tcgaagtta atattttta ataacaata attctatcg atcaacaag tcatcaaaa ttatggtta taggactta gactagtta aggccaatg ataatttta gtattggat gttgggctg gacgagaca atattatgc tgtgtaaga caatagatc ctccttaaa aatatgtaa tgacaataa atgtaactg tagacaata taacgccgg actttacaa cggcttgtc ttatctagt gttaaaaat aaatgtgaa ttttaaaa
- >TC006787 RB
tttcccaaa ctgtataaa accgacgtc cggcgtcgc gacgactgt gagcgtcgg tcgacggtt gatgaggac gtgttgcat ctgaagcaa cagttttgt gtgtctgac gccgctatt ttttcaaat catgctttt cggtgcgcc gaagttttt ttttgggac cggtgtatt ggtgtttgc cttaatttt tgtggtaaa atcggacga atcatgtga tatagaaat agaaaacac tctaaaaac cttactaac accacaaga ctacccatc gatgcagca gcaggcgct tcgcaaaga cttgttgcg tagatttca acaggcgct cgatcgagc cgacgacga agacgaaat gaattacaa aaggtcgat aagttcgct agcacaatg gggtaattt acccggaaa acgtaactt ggaagcact ggccagagc cggctatat ccgcactct acccaatcc cgacgagga agatcccaa caataaaag aagtctctc gactttggc caaaaacga ccaattacc caccacgtt ccaaaataa cgaaagcaa acggggaat cgagtcgtt ggcacgaaa cggcgaatt acacaacag gagagacat ccaagaact gttggacga actctacaa caagcggaa cgttggcag tctggcccg gaattttaa tttccccag ttatggcaa acgatattt ggcgagttt ggtgcgaaa cggcgaact caacgggaa aaggaacat cgcctcgat caaagccca atatcccgg cactaggag taaaagaca ggcgagtta ttacgacgg ggagggtgg cgagttctc cctcccggt gtatcaaaa ccagaacgt ggacgatta tgaggagct cgtgaaagc gttggcggc ggcttatcc taataacga caagcgatt cctagggag gctgccaca gatgggcaa accgaagac gacgccttc gccgaagac cagatttca ctagaggat aagaggttt ctactgcaa cctgctgtc gatcaaata ttgttaagg aagatattt atgcatccg aggacacat ccggcgatt aattagttt ggttttttg gcacatatc tcgataatt tttgcatat ctccgatat atcagactc acttcgaag ttaatattt ttaataaca ataattcta tcgatcaac aagtcatca aaattatgg ttataggac ttagactag ttaaggcca atgataatt ttagtattg gatgttggg ctggacgag acaatatta tgctgtgta agacaatag atcctcctt aaaaatatg taatgacaa taaatgtaa ctgtagaca atataacgc cggacttta caacggctt gtcttatct agtgttaaa aataaatgt gaattttaa aa
- >TC006787 RC
tttcccaaa ctgtataaa accgacgtc cggcgtcgc gacgactgt gagcgtcgg tcgacggtt gatgaggac gtgttgcat ctgaagcaa cagttttgt gtgtctgac gccgctatt ttttcaaat catgctttt cggtgcgcc gaagttttt ttttgggac cggtgtatt ggtgtttgc cttaatttt tgtggtaaa atcggacga atcatgtga tatagaaat agaaaacac tctaaaaac cttactaac accacaaga ctacccatc gatgcagca gcaggcgct tcgcaaaga cttgttgcg tagatttca acaggcgct cgatcgagc cgacgacga agacgaaat gaattacaa aaggtcgat aagttcgct agcacaatg gggtaattt acccggaaa acgtaactt ggaagcact ggccagagc cggctatat ccgcactct acccaatcc cgacgagga agatcccaa caataaaag aagtctctc gactttggc caaaaacga ccaattacc caccacgtt ccaaaataa cgaaagcaa acggggaat cgagtcgtt ggcacgaaa cggcgaatt acacaacag gagagacat ccaagaact gttggacga actctacaa caagcggaa cgttggcag tctggcccg gaattttaa tttccccag ttatggcaa acgatattt ggcgagttt ggtgcgaaa cggcgaact caagtacag cgggaaaag gaacatcgc ctcgatcaa agcccaata tcccggcac taggagtaa aagacaggc gagttatta cgacgggga gggtggcga gttctccct cccggtgta tcaaaacca gaacgtgga cgattatga ggagctcgt gaaagcgtt ggcggcggc ttatcctaa taacgacaa gcgattcct aggctcggt ggctaggag cggctggtt tcggccaaa ttcccgcta ccggtcgcc tgaaaagcg ccacattgg cgctttggc acgtcttgg gtggctgcc gacgctgcg aaacgtccg gagattcaa ccgctccgg caggtccac cactttgga gggctgcag ggaggctgc cacagatgg gcaaaccga agacgacgc cttcgccga agaccagat ttcactaga ggataagag gtttctact gcaacctgc tgtcgatca aatattgtt aaggaagat atttatgca tccgaggac acatccggc gattaatta gtttggttt tttggcaca tatctcgat aatttttgc atatctccg atatatcag actcacttc gaagttaat atttttaat aacaataat tctatcgat caacaagtc atcaaaatt atggttata ggacttaga ctagttaag gccaatgat aattttagt attggatgt tgggctgga cgagacaat attatgctg tgtaagaca atagatcct ccttaaaaa tatgtaatg acaataaat gtaactgta gacaatata acgccggac tttacaacg gcttgtctt atctagtgt taaaaataa atgtgaatt ttaaaa
