Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02884 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
Days post injection
- >TC034185 RA
MFEPKAIFI IILTRASPH IFQNLAQHF IYKVVVLTP QNDLLTYDP FVIEDALAK GVAPKNLGK CINFDPSRK IFTKSLPFY WRNTTVSTT FIQHYPYVF LEGGSLKGQ FFDILDVVA KKLQFQVNL TEFRSQNNT SEAALSFLQ AGGSHMMLN HRRTDVHNY FKFDLSQIN LPAHFYWFA PNCSYSPVW RIFFRQFDF LTLFFTTFF LVFLSLIWY KVSERNDLV YTTLLNLLM LVEAPIFEF AKIRKISGR ILATSMFGF LILSAVGKN ELLRSFTTH LTQCPYKNL QDIIKNNLK CYVSSDMKD YYQFHDEVH IKSFIHQMP EIVVITATE IGQDLTTLE KTELLNPRA KFVIISNET AKFPELFQT LALYFIYKV VVITQGREI VTYDPFRNE NINNKVEPY PLCDCFNHN LAKIFDKKL PKFWRNTTV RVIFSEHFP YLYYEKGAL LGENFKVLN LIKEKLKFK LVIEELDEH KSWRFVDLL QDRKFHQIA NLQTLKVHN HLKVDLVPI DLAAHFYWV VPNGGQVPY WRIFLQNVD PLVWLSIVF FQLVVTLLW CFIEGKDLL YSFFTHFQL FSESNSFNV LKIRKTSSR VFIASSMFA CLILSTSFK SEMLNSFTT IIYDYPIKS LDDIIAKNL SCFVAQDMK LLYKSVGDF YYKYVSSCE VLDDLDDQQ DILLKIALE KKSATISRN LKFKFAANT LLQMGYKKP PMFLIRKQV KFDFLYIYL TKGFPLYDR ITEVVSRMH SSGFTAYFR RIVDYKIAS ALKSNEKIQ SKNLELENV SMGFYTRKA QILMNFRQA NLAAYFDFE LVPLNLGAT YSWVVPKAR RVPFWKSIF LTFNIFLWL VILVFLVLL ATTWRFLQN QGFVTSFLT IFQLILEST TVEITRLRT NSARIVIYS TLLPFLVIS NMFKSEMIR SLTSFSFEK QINSLRDIV ENGLSCSVT NTVKLSYFH SNDPYKDYV QNCAVHQFE EKKDEIFDR IAFKRDHAT VSRTTQFKF ALSKFYNMG HKEPLLHMV PGPMAYYYI YVAKGSPFY HRFLDISSR LQAFGFKKM LFERNKSKA ETVLKIHER IKTDDLTIE HIAVAFYLW FFGIGLAFV AFLGEIIDK KRVSNKRPD LYVITVKGD LKPTLEYLA SFDIFNPRA QFIILIKTT NYKFYFREL AQFFIYRAI LVDTKGNIV TNNIKIIRC TQAKEFPKD LTHHLWKNT TIPVVFYQD YPYLYVKNG KLGGFFYNH FTTVQKFLK FRTEMFEFV NGGYDNRST TLELLKSRK YAVMINFEN ANYYTQFDS VSLNLGMQY FWVVLKADY SPGWKVLYR SFDLFTWLS ILFFLIFLS FFHKKNDLV RMILLHYQL LLENGLNLN NLRKNWITL ATLFAFMVL TTTFQTLMI VSFEKRPKE RQINTLRGI IDSGMKCYT SAEVKLLYA TLKEPFKSY IGNCTTLTD THRNLLVQE FAQKKPTAV TLRRIESKH FAYKFYEMG FEDGLLYNI PARIKLDPA YIYFAKGHP LVARYRQIV ARMFDCGFR KYLLAKADY KIDRLLQAK SYIRPHNLT FEQISVAFY VLLAGLGVA IAVFLAEIL DATEKCIEK IINFSYKPD SLIYLIDTG LKLPFPVVY QTSSSPVLQ LFEYQKPQF YVISDKTNL ANLTKFEIF NPRAKFILL VNSTEYGTY FQELANYYV HSVVLVTLD GQVITPSHK ILNCLDNKL GVFDQGNTQ QGNSSHKVI FFQDYPYVF YDRRNNLRG FFNRFLKTV SEGLKIELK IVENLQNET DNRTAALER LLRREFPFM LNFNTAKRT DFYKFDLVP LYLTFYFYW VVPKSEPLP AWKSLHRNF DICTWTLII STLLILSLL RRLLIKKNL NNSFLLHYQ ILVENSNFA VHGIRKSST RLVVFLTIF SFLILSTSF KTLMIRSFT NDLREQQID SFGQIVESQ MSCFTTDEV KEIYDLWYV SNCATLSDR NKKQVLARF ARKVPTALT LRRIEYKYA EYEFYLMGF NEPLLHLIR QRIKLDYAY IYFSKGHPL YFRFKELVK RLANSGISS WLVSKANYR IDRILKTKS AIFSKTLDF HQISVAFYL LVTGLSVSV LVFSVEITK MKLLFACIS LTLCVNESH FIDMETTCV ERIITKFPN SVIYTIDTN LKLNYPVIY QTTIRQYLT LFQFRYPDL YVIDLEALD IDQMLQFLE QFDIFNSRA KYIILSKNA KPGVILQKL LKYFIYNAV VLTPNGTTV TFNPFINEN VSPDDIQPI VLGSCLELE PSELFNTYL PKVWRNTTI PAVFFNMYP FLYADQNNE IQGMAFRNF KLIQDVLKF RINVTRTKV GAFGAVRIN RTYTNVLGL LQTRQQHLM LNNFMLLTS QQVLEFDLT TFWGVLSQH WVVPKAQPV PDWLAIFYA FNPIIWVLV FFSLIVLSV VWHYFGNDS LLESLELHF QILLESSNS KVLKITPLS SRVLACVAI FSFYVVSTA FRREMVRIF TSYLYQDQT NSLKGLVKK RIPCTVYGE IKELYQSKE EIFGDYVNN VCSTIVPNT DIADTFTRI ASGENLATV LTKMTFKSL KNRMYLKGG KEPQVYLIP KAIKSEFIY SFLAKGHPF HQRFKIVTG RLVSSGITS WFRKQFQQS LEKELESKE IVNADDLSF EEVSAAIYI LFAGFILGF VTYVGYMQF TNPTLLIRD IDLIKQIGV KIFDSFHDH VTAAGIETE PLLNQSLIS LHGEDWKRM RATLSPAFT SSKMKNMYH LIYECSENF AQHFQDGKQ NVEVKDIFS KFTNDVIAT AAFGIQVNS LKEPDNEFY SMGKALTTF QGFQLIKFF FVQLVPKIA EILKVRFLN PKVANFFTS IIINNMQKR ERDGIIRPD LIHLLMEAR KGKLKYDKG NEFGDGFAT VEESEIGKN TKQVELTDD LIVAQALLF FFAGFDTVS TGASFLAHE LATNPDVQK KLQEEIDMV LEENHGKIS YTRIQSMKY LDQVISESL RLWPPAPQT DRFCNTDFV LEPTKPHER RFTIEKGVT TIIPIYGIH RDPQYFPNP DKFDPERFS DENKAKIVP GTYMPFGVG PRNCIAWPL VGNMWSTFI QKKNFNEIS NDIYKKYPN DRYVGYMQF TDPVLLIRD LDLIKQIGV KVFDNFRDH HAVAGFKTE PLFNKSLAF LEGEEWKQM RATLSPAFT SSKMKNMYH LIYECAENF AKHFQGKKQ DVEVKDVFS RFTNDIIAT VAFGIQINS LEERNNEFY LSGKSVTTF SGKQLLKFF AAQVIPQVF EFFKVGVFD QKIVKYFND IIIDNMHKR EKEGIIRPD LIHLLMEAR KGKLKHDNS NEGGDGFAT VEESDIGKN SKKMELTDE LMLFGT
- >TC034185 RA
atgtttgaa ccgaaagca attttcata attatcctg acacgtgcc agtccccat attttccaa aacctggcc caacatttc atttacaaa gttgttgtc ttgacccct cagaacgat ttgttaact tacgacccc tttgtcatt gaggacgcc cttgcaaaa ggagtggcg ccaaaaaat ttgggcaaa tgcatcaat tttgatccc tcgcgtaaa attttcacc aaaagtttg cccttttat tggcgcaac acaaccgtt tccaccact tttatccaa cactaccct tacgttttt ctcgagggc ggctccctc aaagggcaa tttttcgac attttggac gtggtggcc aaaaaattg caatttcaa gtgaatctg accgaattt cgcagtcaa aataacacg tccgaagcc gccctaagt tttttacag gctgggggc tcccacatg atgctaaac caccgtcgg accgacgtc cacaactac ttcaaattc gacctgagt cagatcaat ttacccgcg catttttac tggtttgcc ccgaattgt tcctactcg cccgtttgg cgcattttt ttccgccag tttgacttc cttacgctt tttttcacc acgtttttc ctcgtcttt ttgagcttg atttggtac aaagtctcg gaacgcaac gatttggtt tacaccact ttgctgaac ttgctaatg ctggtggag gctccgatt tttgagttt gcgaaaatt cgcaaaatt tcggggcgc attttggcc acttctatg tttgggttt ttgattctt tcggctgtt ggcaaaaat gagttgctt cgttcgttc acaacgcat ttgactcag tgtccgtac aaaaatttg caggacatt atcaaaaat aatttaaag tgttacgtt agcagtgat atgaaagat tattatcaa tttcacgac gaagttcat attaaaagt ttcattcat caaatgccc gaaatcgtc gtaataaca gccaccgaa atcggtcaa gacctaaca actttggaa aaaaccgag ctattgaac ccgcgagcc aaattcgtg attatctca aacgaaacc gcaaagttc cccgaattg tttcaaaca ctcgctttg tacttcatc tacaaagtt gtggtaatc acccaaggg cgggaaata gtgacttac gaccctttc cgtaacgaa aacataaat aataaagtt gaaccgtac ccactgtgt gactgtttt aaccataat cttgcaaaa atcttcgac aagaagttg ccaaaattt tggcgcaac acaacggtt cgggtcatt ttcagcgag cattttccc tacttgtat tacgaaaag ggggcgcta cttggcgaa aatttcaaa gtcctgaac ttaattaaa gaaaaactc aagtttaag ttagttatt gaagaacta gacgaacac aaatcctgg cgttttgtc gatttgtta caagaccgg aagttccac caaatcgca aatctccaa actttgaag gttcacaac cacttgaaa gttgatttg gtgccaatt gacttggcg gcccatttt tactgggtg gttccaaac gggggccaa gtcccttac tggagaatc tttttgcaa aatgtggac ccccttgtc tggctttca atcgttttt ttccaactt gtggtcaca cttttgtgg tgtttcatc gaaggcaag gacttgctt tactcgttt ttcacccac ttccagctt ttttccgaa tcaaacagt ttcaacgtt ttgaaaatc aggaaaact tcaagccgg gttttcatc gcctcgtcc atgttcgcc tgtttgatt ctttcgacg agtttcaag agcgaaatg ttgaactcg ttcaccaca attatctac gattatccg atcaaaagt cttgacgat attattgcc aaaaacttg tcctgtttt gtggcacaa gacatgaag cttttgtac aaatcagtc ggggatttt tactacaag tacgtctca agctgtgag gttttggac gatttggac gaccaacaa gacatttta cttaaaatc gcgctcgag aaaaaatca gcgacgatt tcgcgcaat ttgaagttt aaatttgcg gccaatacg ttgctacag atggggtac aagaaacca ccgatgttt ttgatcagg aaacaagtc aagtttgat tttttgtac atttatcta acgaagggg tttccccta tacgaccgc attaccgaa gttgtgagt cggatgcat tcgagtggc ttcacagct tacttccgg agaatcgtc gattataaa atagcaagt gctttaaaa tcgaatgag aagattcag tcgaaaaat ttggaactt gaaaacgtc tccatgggg ttttacacg cgcaaagcc cagattttg atgaatttc cggcaagcc aacctggcc gcatatttc gattttgag ttggtacca ttgaatttg ggcgcgacg tattcatgg gtggtacca aaagcccgc cgtgtgccc ttttggaaa tcaattttc ctaactttt aacattttc ctctggttg gttattttg gtgttttta gtattgctt gcaaccact tggcgtttt ttgcaaaac cagggtttt gtcacgtct tttttgaca attttccaa ctgattttg gaatcaaca actgttgaa ataaccaga ctgcggacg aattcagcc agaattgtg atttattca acacttttg ccctttctg gtcatttct aacatgttc aagtcggag atgattcgc tcattgact agtttttcc ttcgagaaa caaattaat tctctcagg gatattgtt gagaatgga ttatcctgc agtgttaca aacaccgtc aaattgtcc tactttcac tcaaatgat ccttacaag gactacgtc cagaactgt gcagttcat cagtttgag gaaaaaaag gatgagatt tttgaccgg attgccttc aaacgtgat catgcaacc gtttcaaga accactcaa tttaaattt gctttgagc aaattttac aacatggga cacaaggaa ccattgctc catatggta ccaggtcct atggcttac tattacatt tatgtggca aaagggagt cctttttac caccgtttt ctcgacatt agtagtcga ttacaagcc ttcgggttt aagaaaatg ttatttgaa cggaacaaa tccaaagct gagactgtt ctaaaaata catgaaagg atcaaaacg gacgatcta accattgaa cacattgct gttgctttc tatttgtgg ttttttggg ataggtctg gcctttgtg gcctttttg ggggaaata atagacaag aagcgagtt tcgaataaa cgaccagat ttgtacgta atcacagtc aagggcgat ttaaaaccc acccttgaa tatctggca agtttcgat attttcaac cctcgggca caatttatt attttaatc aaaacaacc aactacaag ttttatttc cgagaatta gcccagttt tttatttat cgtgcaatt ttggtcgac actaaggga aacattgtt accaataat atcaaaatc ataagatgc actcaagca aaagaattc cctaaagat ttaacacat catttgtgg aaaaatact acaattcca gttgtcttc taccaggat tatccttac ctttatgtg aaaaatgga aaactgggc ggttttttt tacaaccat tttaccacc gttcagaaa tttctaaaa ttccggaca gaaatgttc gagtttgtt aatgggggg tacgacaat cgcagcacc acgttagag ttgttaaaa agtcgcaaa tacgcagtc atgatcaat ttcgaaaat gcaaattat tacacacag ttcgattcc gtttcgtta aacctcgga atgcaatac ttttgggtg gttttaaaa gccgattat tcacccggc tggaaagta ctttaccgc agttttgat ttattcacc tggctttca attctattt tttctaatt tttttgagt tttttccac aagaaaaac gacttggtc cgaatgatt ttactccac taccagtta ctcctcgaa aatggttta aacttgaac aatttacgc aaaaactgg attacttta gccacgtta ttcgctttc atggtctta accacaacc tttcagacc ttgatgatc gtctcattt gagaaacgc ccaaaagaa cgtcaaata aacacactc agagggatc attgattcg ggaatgaag tgttacaca agtgccgaa gtcaaatta ttgtatgcc acgttaaaa gagcccttc aaaagttac attggcaat tgtaccacc ttgacggac acacaccga aaccttcta gtccaagaa ttcgcccag aaaaaaccc acagcggtc acactcaga agaatagaa tcgaaacat ttcgcctac aaattctat gaaatgggt tttgaggac gggcttttg tacaacatt ccggcaagg attaagcta gaccctgcc tatatttat ttcgcaaaa ggacatcca ctggttgcc agatatcgg caaatagtg gctaggatg ttcgactgt gggtttagg aaatatttg ttagccaaa gctgactac aagattgat cggttgctc caagccaaa agctacatt cggccgcat aatctgact tttgagcag atttcggtg gctttttac gttttgtta gccggcttg ggggtcgct atagctgtg tttttggcc gaaattttg gatgcaact gaaaaatgt atcgagaaa ataattaat ttttcgtac aaacccgac tctttgatt tatttaatt gacacaggc ttgaaatta ccattccct gtcgtttac cagacgagt tccagcccc gtattgcaa ttattcgag taccagaaa ccacaattt tacgttatt agtgacaaa acaaacctg gcaaattta accaagttt gagattttt aacccgcgg gcaaagttc atacttttg gtgaattcg actgagtac gggacgtat tttcaagaa ctggccaat tattacgtc cacagtgtg gttttggtg actttggac ggtcaagtc ataacacca agtcacaaa attttaaat tgtttggac aataagttg ggggttttt gaccaggga aacacccaa caggggaac agctcacac aaagtgatt ttttttcaa gactatccg tacgttttt tacgacagg aggaacaat ctgcggggg tttttcaac cgattttta aaaactgtg agtgagggc ttgaaaatt gagctcaaa attgtggaa aacttgcag aatgagact gataatcga actgctgct ttagagcgt ttgcttcgg cgagaattt cccttcatg ctcaatttt aacacagcc aagaggacc gacttttac aaatttgac ttggtccca ctttactta acgttttac ttctattgg gtggttcca aagtctgag cctttaccg gcttggaag tcactgcat cgtaatttt gatatttgc acctggact ttaatcatc tcaactctc ctaattcta tcattactg agacgtctg ttaattaag aaaaacctg aacaactcg tttctcctc cattatcaa attctggtc gaaaatagc aattttgca gttcatggg atacgcaaa agttcgact cgacttgtg gtctttctt acaattttc tccttttta attttatcg acaagtttt aagacttta atgattcgt tcgtttacg aacgatttg cgcgagcaa caaattgac agttttggg caaatagtc gagtcgcaa atgtcgtgt tttacgact gatgaagtt aaggagatt tatgatttg tggtacgtt tcaaattgt gccactttg agtgatcgc aacaaaaag caagtcctt gccaggttt gcccggaag gttccaact gcgttaaca cttcgaagg attgagtac aaatacgca gaatatgag ttttatttg atgggtttt aatgaacct ctcttgcat ttgataaga caaaggatt aagctggat tatgcctat atttatttt tcgaagggc catccactt tatttcagg tttaaggag cttgtgaaa cgattggcc aattcgggg atttccagc tggcttgtc tcaaaagcc aattaccgg attgatcga atactaaaa accaaaagt gcgattttt tccaaaact ctcgacttt caccaaatt tcggtcgct ttttattta ctagtgaca gggttgtcg gttagtgta ctagttttt agtgtagaa attacaaaa atgaaacta ctctttgcc tgtatttct ttgaccctt tgtgttaac gaatcccac tttatcgac atggaaaca acatgtgtt gagagaatt atcacaaaa tttccaaat tctgtgatc tacacaatt gacacaaac ctcaaactt aactatccg gtgatttac caaaccacc attcgacaa tacctgact ttgttccaa ttccgctat cccgacctc tacgttatc gatttggaa gcgctggac attgaccaa atgttgcaa tttttggag cagtttgac attttcaac tcgcgggct aaatacatc attttgagc aaaaacgcc aaacctgga gtgattttg cagaaatta ttaaagtac tttatttac aacgctgtt gttttaaca ccaaatggt accacagtg actttcaac cctttcatc aacgaaaac gtctcacct gacgacatt caaccaata gttttgggt tcttgttta gaactagaa ccaagcgaa ctttttaac acttatctc ccaaaagtt tggaggaac acaacaatc ccagccgtt ttcttcaac atgtaccca tttttgtac gccgaccag aacaacgaa atccaagga atggccttc cggaacttc aaactgatc caagacgtg ctaaaattc cggataaac gtcacccgg actaaagtt ggggcgttc ggagctgtc cgaatcaac cggacttac accaacgtg ctaggtttg ttgcagact cgccaacaa cacctaatg ctcaacaac ttcatgttg ctgacaagc cagcaagtg ctggagttt gatttgacc actttttgg ggcgttttg tcccaacac tgggtggta cctaaagcc cagcctgtg cccgactgg cttgcaatt ttctacgcc tttaacccg atcatttgg gtgctagtt ttcttttcc ttgattgtg ttaagtgtg gtttggcat tattttggc aacgacagt ttgctggag tctttggag ctccacttc cagattttg ctcgaatct agcaattct aaagtactc aaaattaca ccgttgtca agtcgggtc ttggcctgt gttgcaatt ttttccttt tatgtcgtt tcgacggct ttcaggcgc gaaatggtc cggattttt acgagttat ttgtaccag gaccagaca aattccttg aaaggttta gttaagaag cgaattccg tgcactgtt tatggagaa attaaggaa ttgtatcaa tccaaagag gagattttt ggtgattat gtcaataat gtgtgttcg acgattgtt ccaaatacc gatattgcg gacactttt actagaatt gcctctggg gaaaatctg gccacggtt ttgacgaaa atgacgttt aaaagtctc aagaaccgg atgtacctc aaagggggg aaagagccg caagtttac ttaattccg aaagcgatc aagtccgag tttatttat agttttttg gcgaaagga cacccgttc caccaacgg tttaaaata gtgactgga cggttggtt tcgtctgga attacaagt tggttcagg aaacagttt caacagtcg cttgaaaaa gagttggaa agtaaagaa attgtgaat gcggatgat ctgtcgttt gaggaagtt tcagcagcg atttatatt ctctttgct gggtttatt ttgggtttt gttacatac gtgggttat atgcagttt acaaacccg actctttta atccgtgac attgatttg atcaaacaa attggagtg aaaattttc gacagtttt cacgaccac gtcactgca gctgggatt gaaactgaa cctttgctc aaccaaagt cttattagt ttacatgga gaggactgg aagcgaatg agggcaacc ctaagcccg gctttcact agcagtaaa atgaaaaat atgtatcat ttaatctac gaatgttcc gaaaatttc gcccaacat tttcaagat gggaaacag aacgttgaa gttaaggac attttctcg aagtttacc aacgatgtg attgcaact gcggcgttc ggaatccaa gtaaattcg ttaaaagaa cccgacaat gagttttat tcgatggga aaagcactg actacgttt caaggtttt cagctcatc aagtttttt tttgttcaa ttagtgccc aaaattgct gaaatcctg aaggtgcgt tttttgaat ccaaaggtg gcaaatttt ttcacaagt atcattatt aataatatg caaaaaagg gagcgagat ggaattatt cggcccgat ttaattcat ctgttaatg gaagcgaga aaagggaaa ctcaagtat gataaaggt aacgaattt ggtgatggt tttgccacg gttgaggag tcagaaatt ggcaaaaac accaaacaa gttgaacta acagacgat ttaatagtg gctcaagct ttacttttc tttttcgct ggtttcgac actgtttcc acaggtgcc agttttctg gcacatgaa ttagccaca aatcctgac gttcaaaaa aaactacaa gaagaaata gacatggta cttgaggaa aatcacgga aaaatttca tacacaagg atacaatcg atgaaatat ctcgaccaa gttatttcc gaaagttta agactttgg ccaccagct ccccaaacc gatcgtttc tgcaacacc gactttgtt cttgagcca acaaaacca cacgaaaga cgatttacg atcgagaaa ggtgttaca acaattatt ccgatttat ggaatacat agagaccct cagtacttt ccaaatccg gataaattc gatcctgaa cggtttagt gacgaaaat aaggccaaa attgtgcca gggacttat atgccgttt ggtgtcggt ccaagaaat tgcattgca tggcctttg gtcggaaac atgtggagt acatttatc caaaagaaa aatttcaat gaaatatct aacgatatc tacaaaaaa tacccaaac gacagatac gtgggttat atgcagttt acagaccca gtcctccta atcagagac ttggacttg attaaacaa atcggagtg aaagttttc gacaatttt cgcgaccat cacgctgtt gccggtttt aaaacagaa cccttgttc aataaaagt ctcgcattc ctggaagga gaagaatgg aagcaaatg agggccact ttaagccca gctttcact agcagtaaa atgaaaaat atgtatcac ttgatttac gaatgtgcc gaaaatttc gccaaacat tttcaaggc aaaaaacag gacgttgaa gtgaaggat gttttttcg agatttacc aacgatata attgctacg gtggcattt ggcattcaa attaattct ttggaagaa cgtaacaat gagttttat ttgagtgga aaatcggtg actacgttc agcggcaaa caattactc aagtttttt gcagcacaa gtaattccc caagttttt gagtttttc aaagtgggt gtgtttgat caaaaaatt gtaaagtac tttaacgat atcatcatt gacaatatg cacaagagg gaaaaggaa ggaatcatt cggcccgat ttaattcat ttattgatg gaagctcgt aaaggaaaa cttaaacat gataacagt aatgaaggt ggtgatggt tttgccaca gttgaagaa tccgacatt ggcaaaaat tccaaaaaa atggaacta acagacgaa ctaatgctt tttggcaca tga
- >TC034185 RA
actttggaa caaatcgaa atgtttgaa ccgaaagca attttcata attatcctg acacgtgcc agtccccat attttccaa aacctggcc caacatttc atttacaaa gttgttgtc ttgacccct cagaacgat ttgttaact tacgacccc tttgtcatt gaggacgcc cttgcaaaa ggagtggcg ccaaaaaat ttgggcaaa tgcatcaat tttgatccc tcgcgtaaa attttcacc aaaagtttg cccttttat tggcgcaac acaaccgtt tccaccact tttatccaa cactaccct tacgttttt ctcgagggc ggctccctc aaagggcaa tttttcgac attttggac gtggtggcc aaaaaattg caatttcaa gtgaatctg accgaattt cgcagtcaa aataacacg tccgaagcc gccctaagt tttttacag gctgggggc tcccacatg atgctaaac caccgtcgg accgacgtc cacaactac ttcaaattc gacctgagt cagatcaat ttacccgcg catttttac tggtttgcc ccgaattgt tcctactcg cccgtttgg cgcattttt ttccgccag tttgacttc cttacgctt tttttcacc acgtttttc ctcgtcttt ttgagcttg atttggtac aaagtctcg gaacgcaac gatttggtt tacaccact ttgctgaac ttgctaatg ctggtggag gctccgatt tttgagttt gcgaaaatt cgcaaaatt tcggggcgc attttggcc acttctatg tttgggttt ttgattctt tcggctgtt ggcaaaaat gagttgctt cgttcgttc acaacgcat ttgactcag tgtccgtac aaaaatttg caggacatt atcaaaaat aatttaaag tgttacgtt agcagtgat atgaaagat tattatcaa tttcacgac gaagttcat attaaaagt ttcattcat caaatgccc gaaatcgtc gtaataaca gccaccgaa atcggtcaa gacctaaca actttggaa aaaaccgag ctattgaac ccgcgagcc aaattcgtg attatctca aacgaaacc gcaaagttc cccgaattg tttcaaaca ctcgctttg tacttcatc tacaaagtt gtggtaatc acccaaggg cgggaaata gtgacttac gaccctttc cgtaacgaa aacataaat aataaagtt gaaccgtac ccactgtgt gactgtttt aaccataat cttgcaaaa atcttcgac aagaagttg ccaaaattt tggcgcaac acaacggtt cgggtcatt ttcagcgag cattttccc tacttgtat tacgaaaag ggggcgcta cttggcgaa aatttcaaa gtcctgaac ttaattaaa gaaaaactc aagtttaag ttagttatt gaagaacta gacgaacac aaatcctgg cgttttgtc gatttgtta caagaccgg aagttccac caaatcgca aatctccaa actttgaag gttcacaac cacttgaaa gttgatttg gtgccaatt gacttggcg gcccatttt tactgggtg gttccaaac gggggccaa gtcccttac tggagaatc tttttgcaa aatgtggac ccccttgtc tggctttca atcgttttt ttccaactt gtggtcaca cttttgtgg tgtttcatc gaaggcaag gacttgctt tactcgttt ttcacccac ttccagctt ttttccgaa tcaaacagt ttcaacgtt ttgaaaatc aggaaaact tcaagccgg gttttcatc gcctcgtcc atgttcgcc tgtttgatt ctttcgacg agtttcaag agcgaaatg ttgaactcg ttcaccaca attatctac gattatccg atcaaaagt cttgacgat attattgcc aaaaacttg tcctgtttt gtggcacaa gacatgaag cttttgtac aaatcagtc ggggatttt tactacaag tacgtctca agctgtgag gttttggac gatttggac gaccaacaa gacatttta cttaaaatc gcgctcgag aaaaaatca gcgacgatt tcgcgcaat ttgaagttt aaatttgcg gccaatacg ttgctacag atggggtac aagaaacca ccgatgttt ttgatcagg aaacaagtc aagtttgat tttttgtac atttatcta acgaagggg tttccccta tacgaccgc attaccgaa gttgtgagt cggatgcat tcgagtggc ttcacagct tacttccgg agaatcgtc gattataaa atagcaagt gctttaaaa tcgaatgag aagattcag tcgaaaaat ttggaactt gaaaacgtc tccatgggg ttttacacg cgcaaagcc cagattttg atgaatttc cggcaagcc aacctggcc gcatatttc gattttgag ttggtacca ttgaatttg ggcgcgacg tattcatgg gtggtacca aaagcccgc cgtgtgccc ttttggaaa tcaattttc ctaactttt aacattttc ctctggttg gttattttg gtgttttta gtattgctt gcaaccact tggcgtttt ttgcaaaac cagggtttt gtcacgtct tttttgaca attttccaa ctgattttg gaatcaaca actgttgaa ataaccaga ctgcggacg aattcagcc agaattgtg atttattca acacttttg ccctttctg gtcatttct aacatgttc aagtcggag atgattcgc tcattgact agtttttcc ttcgagaaa caaattaat tctctcagg gatattgtt gagaatgga ttatcctgc agtgttaca aacaccgtc aaattgtcc tactttcac tcaaatgat ccttacaag gactacgtc cagaactgt gcagttcat cagtttgag gaaaaaaag gatgagatt tttgaccgg attgccttc aaacgtgat catgcaacc gtttcaaga accactcaa tttaaattt gctttgagc aaattttac aacatggga cacaaggaa ccattgctc catatggta ccaggtcct atggcttac tattacatt tatgtggca aaagggagt cctttttac caccgtttt ctcgacatt agtagtcga ttacaagcc ttcgggttt aagaaaatg ttatttgaa cggaacaaa tccaaagct gagactgtt ctaaaaata catgaaagg atcaaaacg gacgatcta accattgaa cacattgct gttgctttc tatttgtgg ttttttggg ataggtctg gcctttgtg gcctttttg ggggaaata atagacaag aagcgagtt tcgaataaa cgaccagat ttgtacgta atcacagtc aagggcgat ttaaaaccc acccttgaa tatctggca agtttcgat attttcaac cctcgggca caatttatt attttaatc aaaacaacc aactacaag ttttatttc cgagaatta gcccagttt tttatttat cgtgcaatt ttggtcgac actaaggga aacattgtt accaataat atcaaaatc ataagatgc actcaagca aaagaattc cctaaagat ttaacacat catttgtgg aaaaatact acaattcca gttgtcttc taccaggat tatccttac ctttatgtg aaaaatgga aaactgggc ggttttttt tacaaccat tttaccacc gttcagaaa tttctaaaa ttccggaca gaaatgttc gagtttgtt aatgggggg tacgacaat cgcagcacc acgttagag ttgttaaaa agtcgcaaa tacgcagtc atgatcaat ttcgaaaat gcaaattat tacacacag ttcgattcc gtttcgtta aacctcgga atgcaatac ttttgggtg gttttaaaa gccgattat tcacccggc tggaaagta ctttaccgc agttttgat ttattcacc tggctttca attctattt tttctaatt tttttgagt tttttccac aagaaaaac gacttggtc cgaatgatt ttactccac taccagtta ctcctcgaa aatggttta aacttgaac aatttacgc aaaaactgg attacttta gccacgtta ttcgctttc atggtctta accacaacc tttcagacc ttgatgatc gtctcattt gagaaacgc ccaaaagaa cgtcaaata aacacactc agagggatc attgattcg ggaatgaag tgttacaca agtgccgaa gtcaaatta ttgtatgcc acgttaaaa gagcccttc aaaagttac attggcaat tgtaccacc ttgacggac acacaccga aaccttcta gtccaagaa ttcgcccag aaaaaaccc acagcggtc acactcaga agaatagaa tcgaaacat ttcgcctac aaattctat gaaatgggt tttgaggac gggcttttg tacaacatt ccggcaagg attaagcta gaccctgcc tatatttat ttcgcaaaa ggacatcca ctggttgcc agatatcgg caaatagtg gctaggatg ttcgactgt gggtttagg aaatatttg ttagccaaa gctgactac aagattgat cggttgctc caagccaaa agctacatt cggccgcat aatctgact tttgagcag atttcggtg gctttttac gttttgtta gccggcttg ggggtcgct atagctgtg tttttggcc gaaattttg gatgcaact gaaaaatgt atcgagaaa ataattaat ttttcgtac aaacccgac tctttgatt tatttaatt gacacaggc ttgaaatta ccattccct gtcgtttac cagacgagt tccagcccc gtattgcaa ttattcgag taccagaaa ccacaattt tacgttatt agtgacaaa acaaacctg gcaaattta accaagttt gagattttt aacccgcgg gcaaagttc atacttttg gtgaattcg actgagtac gggacgtat tttcaagaa ctggccaat tattacgtc cacagtgtg gttttggtg actttggac ggtcaagtc ataacacca agtcacaaa attttaaat tgtttggac aataagttg ggggttttt gaccaggga aacacccaa caggggaac agctcacac aaagtgatt ttttttcaa gactatccg tacgttttt tacgacagg aggaacaat ctgcggggg tttttcaac cgattttta aaaactgtg agtgagggc ttgaaaatt gagctcaaa attgtggaa aacttgcag aatgagact gataatcga actgctgct ttagagcgt ttgcttcgg cgagaattt cccttcatg ctcaatttt aacacagcc aagaggacc gacttttac aaatttgac ttggtccca ctttactta acgttttac ttctattgg gtggttcca aagtctgag cctttaccg gcttggaag tcactgcat cgtaatttt gatatttgc acctggact ttaatcatc tcaactctc ctaattcta tcattactg agacgtctg ttaattaag aaaaacctg aacaactcg tttctcctc cattatcaa attctggtc gaaaatagc aattttgca gttcatggg atacgcaaa agttcgact cgacttgtg gtctttctt acaattttc tccttttta attttatcg acaagtttt aagacttta atgattcgt tcgtttacg aacgatttg cgcgagcaa caaattgac agttttggg caaatagtc gagtcgcaa atgtcgtgt tttacgact gatgaagtt aaggagatt tatgatttg tggtacgtt tcaaattgt gccactttg agtgatcgc aacaaaaag caagtcctt gccaggttt gcccggaag gttccaact gcgttaaca cttcgaagg attgagtac aaatacgca gaatatgag ttttatttg atgggtttt aatgaacct ctcttgcat ttgataaga caaaggatt aagctggat tatgcctat atttatttt tcgaagggc catccactt tatttcagg tttaaggag cttgtgaaa cgattggcc aattcgggg atttccagc tggcttgtc tcaaaagcc aattaccgg attgatcga atactaaaa accaaaagt gcgattttt tccaaaact ctcgacttt caccaaatt tcggtcgct ttttattta ctagtgaca gggttgtcg gttagtgta ctagttttt agtgtagaa attacaaaa atgaaacta ctctttgcc tgtatttct ttgaccctt tgtgttaac gaatcccac tttatcgac atggaaaca acatgtgtt gagagaatt atcacaaaa tttccaaat tctgtgatc tacacaatt gacacaaac ctcaaactt aactatccg gtgatttac caaaccacc attcgacaa tacctgact ttgttccaa ttccgctat cccgacctc tacgttatc gatttggaa gcgctggac attgaccaa atgttgcaa tttttggag cagtttgac attttcaac tcgcgggct aaatacatc attttgagc aaaaacgcc aaacctgga gtgattttg cagaaatta ttaaagtac tttatttac aacgctgtt gttttaaca ccaaatggt accacagtg actttcaac cctttcatc aacgaaaac gtctcacct gacgacatt caaccaata gttttgggt tcttgttta gaactagaa ccaagcgaa ctttttaac acttatctc ccaaaagtt tggaggaac acaacaatc ccagccgtt ttcttcaac atgtaccca tttttgtac gccgaccag aacaacgaa atccaagga atggccttc cggaacttc aaactgatc caagacgtg ctaaaattc cggataaac gtcacccgg actaaagtt ggggcgttc ggagctgtc cgaatcaac cggacttac accaacgtg ctaggtttg ttgcagact cgccaacaa cacctaatg ctcaacaac ttcatgttg ctgacaagc cagcaagtg ctggagttt gatttgacc actttttgg ggcgttttg tcccaacac tgggtggta cctaaagcc cagcctgtg cccgactgg cttgcaatt ttctacgcc tttaacccg atcatttgg gtgctagtt ttcttttcc ttgattgtg ttaagtgtg gtttggcat tattttggc aacgacagt ttgctggag tctttggag ctccacttc cagattttg ctcgaatct agcaattct aaagtactc aaaattaca ccgttgtca agtcgggtc ttggcctgt gttgcaatt ttttccttt tatgtcgtt tcgacggct ttcaggcgc gaaatggtc cggattttt acgagttat ttgtaccag gaccagaca aattccttg aaaggttta gttaagaag cgaattccg tgcactgtt tatggagaa attaaggaa ttgtatcaa tccaaagag gagattttt ggtgattat gtcaataat gtgtgttcg acgattgtt ccaaatacc gatattgcg gacactttt actagaatt gcctctggg gaaaatctg gccacggtt ttgacgaaa atgacgttt aaaagtctc aagaaccgg atgtacctc aaagggggg aaagagccg caagtttac ttaattccg aaagcgatc aagtccgag tttatttat agttttttg gcgaaagga cacccgttc caccaacgg tttaaaata gtgactgga cggttggtt tcgtctgga attacaagt tggttcagg aaacagttt caacagtcg cttgaaaaa gagttggaa agtaaagaa attgtgaat gcggatgat ctgtcgttt gaggaagtt tcagcagcg atttatatt ctctttgct gggtttatt ttgggtttt gttacatac gtgggttat atgcagttt acaaacccg actctttta atccgtgac attgatttg atcaaacaa attggagtg aaaattttc gacagtttt cacgaccac gtcactgca gctgggatt gaaactgaa cctttgctc aaccaaagt cttattagt ttacatgga gaggactgg aagcgaatg agggcaacc ctaagcccg gctttcact agcagtaaa atgaaaaat atgtatcat ttaatctac gaatgttcc gaaaatttc gcccaacat tttcaagat gggaaacag aacgttgaa gttaaggac attttctcg aagtttacc aacgatgtg attgcaact gcggcgttc ggaatccaa gtaaattcg ttaaaagaa cccgacaat gagttttat tcgatggga aaagcactg actacgttt caaggtttt cagctcatc aagtttttt tttgttcaa ttagtgccc aaaattgct gaaatcctg aaggtgcgt tttttgaat ccaaaggtg gcaaatttt ttcacaagt atcattatt aataatatg caaaaaagg gagcgagat ggaattatt cggcccgat ttaattcat ctgttaatg gaagcgaga aaagggaaa ctcaagtat gataaaggt aacgaattt ggtgatggt tttgccacg gttgaggag tcagaaatt ggcaaaaac accaaacaa gttgaacta acagacgat ttaatagtg gctcaagct ttacttttc tttttcgct ggtttcgac actgtttcc acaggtgcc agttttctg gcacatgaa ttagccaca aatcctgac gttcaaaaa aaactacaa gaagaaata gacatggta cttgaggaa aatcacgga aaaatttca tacacaagg atacaatcg atgaaatat ctcgaccaa gttatttcc gaaagttta agactttgg ccaccagct ccccaaacc gatcgtttc tgcaacacc gactttgtt cttgagcca acaaaacca cacgaaaga cgatttacg atcgagaaa ggtgttaca acaattatt ccgatttat ggaatacat agagaccct cagtacttt ccaaatccg gataaattc gatcctgaa cggtttagt gacgaaaat aaggccaaa attgtgcca gggacttat atgccgttt ggtgtcggt ccaagaaat tgcattgca tggcctttg gtcggaaac atgtggagt acatttatc caaaagaaa aatttcaat gaaatatct aacgatatc tacaaaaaa tacccaaac gacagatac gtgggttat atgcagttt acagaccca gtcctccta atcagagac ttggacttg attaaacaa atcggagtg aaagttttc gacaatttt cgcgaccat cacgctgtt gccggtttt aaaacagaa cccttgttc aataaaagt ctcgcattc ctggaagga gaagaatgg aagcaaatg agggccact ttaagccca gctttcact agcagtaaa atgaaaaat atgtatcac ttgatttac gaatgtgcc gaaaatttc gccaaacat tttcaaggc aaaaaacag gacgttgaa gtgaaggat gttttttcg agatttacc aacgatata attgctacg gtggcattt ggcattcaa attaattct ttggaagaa cgtaacaat gagttttat ttgagtgga aaatcggtg actacgttc agcggcaaa caattactc aagtttttt gcagcacaa gtaattccc caagttttt gagtttttc aaagtgggt gtgtttgat caaaaaatt gtaaagtac tttaacgat atcatcatt gacaatatg cacaagagg gaaaaggaa ggaatcatt cggcccgat ttaattcat ttattgatg gaagctcgt aaaggaaaa cttaaacat gataacagt aatgaaggt ggtgatggt tttgccaca gttgaagaa tccgacatt ggcaaaaat tccaaaaaa atggaacta acagacgaa ctaatgctt tttggcaca tgaattggc cacaaaccc tgacgttca gaggaaact acaagaaga aatagactc ggtactt
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