Status
Please wait ...
Gene information
General information
TC005373 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13957881..13968988
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005373
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02883

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality late (L7) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005373 RA
      MVRGGRDRS RSPDRKRRR SRSRSKDRS RSSRHRRSR 
      SRERRSRSR DRRERDRRS YSRDRDRRR GDSKEKRSS 
      GKSRRRDKS RSRSRSTDR KKDDSGLAF DPANLDKEE 
      EQKKLELEM QKRRERIER WRAERKKKE EEMKKDAPN 
      ATTTTDEAT AKKWSLEND SEDEDKDKG ETKEDQVEE 
      ETNGEAPPQ EEKQEAKEE KKEEEEEEE EVDPLDEYM 
      KGVQEEVRK THKLDMKKP KTESGNKKG ALVIVTGVA 
      KSKVNKNKG ELIEQNQDG LEYSSEEEI EDLKDTAAS 
      IVNKQKKEL AKIDHNMVR YLPFRKNFY VEVPEIAKM 
      THEEVEAYK EELEGIRVK GKGCPKPIK TWAQCGVST 
      KELNILKKL GFEKPTPIQ VQAIPAIMS GRDLIGIAK 
      TGSGKTLAF LLPMFRHIL DQPPLEELD GPIAIIMTP 
      TRELCMQIG KDIKKFTKS LNLHAVCVY GGTGISEQI 
      AELKRGAEI IVCTPGRMI DMLAANGGK VTNLRRVTY 
      IVLDEADRM FDMGFEPQV MRIIDNVRP DRQTVMFSA 
      TFPRQMEAL ARRILQKPI EVQVGGRSV VCKDVEQHV 
      VILEEDQKF LKLLELLGL YHEHGSIIV FVDKQENAD 
      ILLKELMKA AYNCLSLHG GIDQFDRDS TIIDFKSGK 
      VKLLIATSV AARGLDVKH LILVVNYDC PNHYEDYVH 
      RCGRTGRAG NKGFAYTFI TPEQSRYAG DIIRAFELA 
      SVPIPDPLR ALWDSYKSK QEAEGKKVH SGGGFSGKG 
      FKFDENEAA AVTERKKFQ KAALGLADS DDEDLEHDI 
      DQHIESMFA TKRTVKEIK APLGLINPA AMNTQTAMD 
      KLELAKRLA SRINFSKNI SLDTKFATQ QAAESILKG 
      SSSSLPITA KTVAEQLAA KLNTKLNYQ PKDDDERDD 
      IDNEQTFRK YEEELEIND FPQQARWRV TSKEALAQI 
      SEYSEAGIT VRGTYVPNG KNPPEGERK LYLAIESTS 
      DIAVAKAKA EITRLIKEE LLKLQTAGH HSFNKARYK 
      VV
    CDS sequence
    • >TC005373 RA
      atggttcgg ggcggacgc gacaggagc cgttctccc 
      gaccggaag cggcgccgt tcgcgctcc aggagcaaa 
      gacaggtcc aggtctagc cgccaccgt cgaagtagg 
      tcccgagag aggcgcagc aggtccaga gacaggaga 
      gagcgtgac aggcgttcg tacagtcga gaccgagac 
      cgtcgaaga ggtgacagt aaggagaag cgcagttct 
      ggcaaatcc agacgtcgg gacaagtcc aggtctcgc 
      tccagatcc acagaccgg aagaaggat gattcaggt 
      ctggctttt gatccggcg aaccttgac aaggaagaa 
      gagcaaaaa aagttggaa cttgagatg caaaaacgc 
      cgcgaacgc attgagcgt tggcgcgcc gaacgcaag 
      aagaaagag gaagaaatg aagaaagat gcaccaaat 
      gcaaccaca acaactgac gaagccact gccaaaaaa 
      tggtcccta gaaaacgac tccgaagac gaggataaa 
      gacaaaggc gaaacgaag gaggatcaa gtcgaggaa 
      gaaacaaac ggggaagca ccgccacag gaagaaaaa 
      caagaagcg aaagaagag aaaaaagaa gaagaagaa 
      gaagaagag gaagtcgat cctctagac gagtatatg 
      aaaggagtt caagaagaa gtgagaaag actcacaaa 
      cttgacatg aaaaagccc aaaaccgag tccgggaat 
      aaaaaaggt gccttagtt attgtaaca ggggtggcc 
      aaatccaag gttaataaa aacaaaggt gagttgatt 
      gagcagaat caggacggt ctggagtac tcctctgaa 
      gaggaaatt gaggacttg aaagacaca gctgcgagt 
      attgttaat aagcagaag aaggaattg gcgaaaatt 
      gatcataac atggttaga tatttaccg tttcggaag 
      aatttctac gtagaagtg cccgaaatt gccaagatg 
      acgcacgaa gaggtggag gcctacaag gaggagttg 
      gaggggatt cgagtcaaa ggcaagggc tgccctaag 
      cccatcaaa acatgggcc caatgcggc gtctcgacc 
      aaagagctc aacatcctc aaaaagctc ggtttcgaa 
      aaacccacc ccaattcaa gtccaagcc attcctgca 
      ataatgtcc ggtcgtgat ttaatcgga atcgcgaaa 
      accggaagc ggaaaaacc cttgctttc ctcctcccc 
      atgttccgc cacatcctt gaccaacct ccgctggag 
      gaactcgac ggccctatc gccatcata atgaccccc 
      acacgcgaa ctgtgcatg caaatcggc aaagacatc 
      aaaaaattc accaagagc ttgaatttg cacgcggtt 
      tgcgtttac ggcggcact ggcatttcg gaacagata 
      gccgagttg aagcgcggg gcggaaatt atcgtatgt 
      accccggga cgcatgatt gatatgttg gcggcaaac 
      ggcggcaaa gtcacgaat ttgcgacgc gttacttac 
      attgtgctt gatgaagcc gatcggatg tttgatatg 
      gggttcgag ccgcaagtg atgcggatt attgataat 
      gtgagacct gaccgccaa acggtgatg ttcagtgcg 
      acttttccg cgccaaatg gaggcgcta gccaggcgg 
      attttgcaa aagccgatc gaggtccag gttggtgga 
      cgctcggtt gtgtgcaaa gatgtggaa caacacgtt 
      gttattctg gaggaggac cagaaattc cttaaatta 
      ttggagctg ttgggtctc taccatgag cacgggagt 
      atcattgtc tttgtcgat aagcaggaa aacgccgat 
      attttgtta aaagaattg atgaaagcg gcgtacaat 
      tgcttgagt cttcacggt ggtatcgac caattcgat 
      cgcgattcg acgattatt gattttaag tcggggaaa 
      gtcaaactt ttgattgct acttcggtg gcggctcga 
      gggctagat gtcaagcat ttgatcctg gtggttaat 
      tacgactgt cctaatcac tatgaagat tacgtccac 
      aggtgtggt cggacagga cgtgcaggt aacaagggc 
      ttcgcctac accttcatc acccccgaa cagtcgcga 
      tatgctgga gatataatc cgagcgttt gaactagct 
      tcagtcccc atccctgac cccctgcgt gcactttgg 
      gactcctac aagtccaag caagaagcc gagggcaaa 
      aaagtgcac tccgggggt ggtttcagt ggcaaaggc 
      ttcaaattc gacgagaat gaagctgcc gcagtcacc 
      gaacgaaag aaattccaa aaagcggcc ttaggtctt 
      gccgattcc gacgatgag gatttggag catgacata 
      gaccaacat atcgagagt atgtttgcc acaaaacgg 
      acggttaaa gagattaaa gcaccgtta ggattgatt 
      aatccagct gccatgaat acgcaaacg gcaatggat 
      aagctagag ttggcgaaa agattagct tcaaggatt 
      aatttttcg aagaatatt agtctggat acgaagttt 
      gccacgcag caagctgct gaaagtata ctgaagggg 
      agcagcagc tcgttgccg attacggcg aagacggtg 
      gcggaacag ttggccgcc aaattgaat accaagctg 
      aattatcag ccgaaggac gatgatgag agggatgat 
      attgataat gaacagacg tttaggaag tatgaggag 
      gagttggag attaatgat tttccgcag caggcgagg 
      tggagggtg acgtcaaag gaggcctta gcccagatt 
      tccgaatat tccgaggct gggattacc gtccgtggt 
      acttacgtt cccaacggt aaaaatccg ccagaaggt 
      gaaagaaaa ctgtacctg gccatcgaa agtacgtcc 
      gatatcgct gtggctaaa gccaaagct gaaataacg 
      cgcttgatt aaggaggag ttgcttaaa ctgcaaact 
      gccggtcac cactcgttc aacaaagct agatacaag 
      gtggtgtga
    mRNA sequence
    • >TC005373 RA
      aaactgtaa ccgtgtaaa taaagatta ggttatgtt 
      tggggcaca aaatcttac ttttagttg atttaggtc 
      caattttaa gaaaaacta aagacttac gatggttcg 
      gggcggacg cgacaggag ccgttctcc cgaccggaa 
      gcggcgccg ttcgcgctc caggagcaa agacaggtc 
      caggtctag ccgccaccg tcgaagtag gtcccgaga 
      gaggcgcag caggtccag agacaggag agagcgtga 
      caggcgttc gtacagtcg agaccgaga ccgtcgaag 
      aggtgacag taaggagaa gcgcagttc tggcaaatc 
      cagacgtcg ggacaagtc caggtctcg ctccagatc 
      cacagaccg gaagaagga tgattcagg tctggcttt 
      tgatccggc gaaccttga caaggaaga agagcaaaa 
      aaagttgga acttgagat gcaaaaacg ccgcgaacg 
      cattgagcg ttggcgcgc cgaacgcaa gaagaaaga 
      ggaagaaat gaagaaaga tgcaccaaa tgcaaccac 
      aacaactga cgaagccac tgccaaaaa atggtccct 
      agaaaacga ctccgaaga cgaggataa agacaaagg 
      cgaaacgaa ggaggatca agtcgagga agaaacaaa 
      cggggaagc accgccaca ggaagaaaa acaagaagc 
      gaaagaaga gaaaaaaga agaagaaga agaagaaga 
      ggaagtcga tcctctaga cgagtatat gaaaggagt 
      tcaagaaga agtgagaaa gactcacaa acttgacat 
      gaaaaagcc caaaaccga gtccgggaa taaaaaagg 
      tgccttagt tattgtaac aggggtggc caaatccaa 
      ggttaataa aaacaaagg tgagttgat tgagcagaa 
      tcaggacgg tctggagta ctcctctga agaggaaat 
      tgaggactt gaaagacac agctgcgag tattgttaa 
      taagcagaa gaaggaatt ggcgaaaat tgatcataa 
      catggttag atatttacc gtttcggaa gaatttcta 
      cgtagaagt gcccgaaat tgccaagat gacgcacga 
      agaggtgga ggcctacaa ggaggagtt ggaggggat 
      tcgagtcaa aggcaaggg ctgccctaa gcccatcaa 
      aacatgggc ccaatgcgg cgtctcgac caaagagct 
      caacatcct caaaaagct cggtttcga aaaacccac 
      cccaattca agtccaagc cattcctgc aataatgtc 
      cggtcgtga tttaatcgg aatcgcgaa aaccggaag 
      cggaaaaac ccttgcttt cctcctccc catgttccg 
      ccacatcct tgaccaacc tccgctgga ggaactcga 
      cggccctat cgccatcat aatgacccc cacacgcga 
      actgtgcat gcaaatcgg caaagacat caaaaaatt 
      caccaagag cttgaattt gcacgcggt ttgcgttta 
      cggcggcac tggcatttc ggaacagat agccgagtt 
      gaagcgcgg ggcggaaat tatcgtatg taccccggg 
      acgcatgat tgatatgtt ggcggcaaa cggcggcaa 
      agtcacgaa tttgcgacg cgttactta cattgtgct 
      tgatgaagc cgatcggat gtttgatat ggggttcga 
      gccgcaagt gatgcggat tattgataa tgtgagacc 
      tgaccgcca aacggtgat gttcagtgc gacttttcc 
      gcgccaaat ggaggcgct agccaggcg gattttgca 
      aaagccgat cgaggtcca ggttggtgg acgctcggt 
      tgtgtgcaa agatgtgga acaacacgt tgttattct 
      ggaggagga ccagaaatt ccttaaatt attggagct 
      gttgggtct ctaccatga gcacgggag tatcattgt 
      ctttgtcga taagcagga aaacgccga tattttgtt 
      aaaagaatt gatgaaagc ggcgtacaa ttgcttgag 
      tcttcacgg tggtatcga ccaattcga tcgcgattc 
      gacgattat tgattttaa gtcggggaa agtcaaact 
      tttgattgc tacttcggt ggcggctcg agggctaga 
      tgtcaagca tttgatcct ggtggttaa ttacgactg 
      tcctaatca ctatgaaga ttacgtcca caggtgtgg 
      tcggacagg acgtgcagg taacaaggg cttcgccta 
      caccttcat cacccccga acagtcgcg atatgctgg 
      agatataat ccgagcgtt tgaactagc ttcagtccc 
      catccctga ccccctgcg tgcactttg ggactccta 
      caagtccaa gcaagaagc cgagggcaa aaaagtgca 
      ctccggggg tggtttcag tggcaaagg cttcaaatt 
      cgacgagaa tgaagctgc cgcagtcac cgaacgaaa 
      gaaattcca aaaagcggc cttaggtct tgccgattc 
      cgacgatga ggatttgga gcatgacat agaccaaca 
      tatcgagag tatgtttgc cacaaaacg gacggttaa 
      agagattaa agcaccgtt aggattgat taatccagc 
      tgccatgaa tacgcaaac ggcaatgga taagctaga 
      gttggcgaa aagattagc ttcaaggat taatttttc 
      gaagaatat tagtctgga tacgaagtt tgccacgca 
      gcaagctgc tgaaagtat actgaaggg gagcagcag 
      ctcgttgcc gattacggc gaagacggt ggcggaaca 
      gttggccgc caaattgaa taccaagct gaattatca 
      gccgaagga cgatgatga gagggatga tattgataa 
      tgaacagac gtttaggaa gtatgagga ggagttgga 
      gattaatga ttttccgca gcaggcgag gtggagggt 
      gacgtcaaa ggaggcctt agcccagat ttccgaata 
      ttccgaggc tgggattac cgtccgtgg tacttacgt 
      tcccaacgg taaaaatcc gccagaagg tgaaagaaa 
      actgtacct ggccatcga aagtacgtc cgatatcgc 
      tgtggctaa agccaaagc tgaaataac gcgcttgat 
      taaggagga gttgcttaa actgcaaac tgccggtca 
      ccactcgtt caacaaagc tagatacaa ggtggtgtg 
      aatacttta cgtcttgag catacatat ttttaaagt 
      tgtctgtat agtgcgtac gaatggata tttatttag 
      gaaaataaa ttttttcat cgact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY