Status
Please wait ...
Gene information
General information
TC006704 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13082277..13089089
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006704
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02881

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature potentially partiallydegenerated - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    head partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    prothorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006704 RA
      MSNAPTSRR NGAQKIRLT ILCARNLVR KDLFRLPDP 
      FAKISVDGS GQCHSTEFV KNTLEPKWN SHYDLYIGR 
      NDSITISVW NHRKVIKKQ GSGFLGCVR IVNSHIQRL 
      RDTGYQCLD LGKACPADS EPVKGQIII SLLSRDGPC 
      SGTPLAVVG PQGELRGPT DRDSSPSNN DDLPPGWEE 
      RRTENGRPY YVNHITRST QWIKPQSTN KTRRRSTHQ 
      NDNNNTNNE NTIGGTQMG VQEILNNNT GETLTPSSS 
      TSPVSPVVS PQKTTSTPQ TTDNRNNFI TLSPTSTSV 
      IPAPNNISC NAPNGENTN VVSPQMGQG QGQRAIVRS 
      ERRQRSAEE RRDGSSRRR SGRNNRNSG GTTAQGPVT 
      QNSKLDLPP GYELRTTQQ GQVYFYHIP TGVSTWHDP 
      RIPKDLAPL SLALDHLGP LPPGWEMRQ TASGRIYFV 
      DHNNRTTQF TDPRLNTQI LNNILRRAS ATSTTTPTT 
      RTTPTTTTA STTSPSTST TTVTTTTTT TAVPQNPAP 
      LSPPPRTRI VEELPQSLL NDCDHLPKY RRDLVAKLK 
      TLRAELTAL QPQSGHCRL EVSRNEVFE ESYRLIMKM 
      RPKDMRKRL MVKFKGEEG LDYGGVARE WLHLLSREM 
      LNPQYGLFQ YSRDDHYTL QINPDSAVN PEHLSYFHF 
      VGRILGIAV FHNHQLEGG FTLPFYKQL LNKPITLQD 
      IEGVDPELH RSLTWMLEN NIDGVLDTT FSVENNSFG 
      IVKVHELKP SGATIPVTE DNKREYVKL YVNYRFMRG 
      IEQQFLALQ KGFTELIPP SLLRPFDER ELELVISGI 
      GSIDIADWR SHTRLKHCT PETPVVQWF WQVVESYSE 
      EMRARLLQF VTGSSRVPL QGFKALQGS TGAAGPRLF 
      TIHCIDCSP QNLPKAHTC FNRIDIPPY DSYQTLADK 
      LTQAVEETC GFAVE
    CDS sequence
    • >TC006704 RA
      atgtccaac gcccccacg agtcgtcga aatggtgcc 
      caaaaaatc cgactgacg attctttgt gcgaggaat 
      ttggtccgg aaggatttg ttccgcctt cccgaccct 
      tttgcgaag atttcggtg gacggtagt ggacagtgc 
      cactccacc gagttcgtt aaaaacacg ttggagccc 
      aagtggaac tcgcactac gacctgtac atcgggcgc 
      aatgattcc atcacgatc tcggtgtgg aaccaccgc 
      aaagtcatc aagaagcag ggcagcggg ttcctgggc 
      tgcgtccgg attgttaat tcgcacatc cagaggctg 
      agggacacc ggataccaa tgtttggat ctgggaaaa 
      gcatgtccg gccgacagc gaaccggtc aaaggtcaa 
      atcatcatt tcgttatta tcacgcgat ggcccttgt 
      agtggtaca cctttggcc gttgttggc ccccaaggc 
      gagttacgg gggccaact gatcgcgat tcttcgccc 
      tcaaataac gacgattta ccgcccggc tgggaggaa 
      cgccgaacg gaaaacgga aggccctac tacgttaat 
      cacatcacc aggtccaca cagtggatt aaaccacaa 
      tcgaccaac aaaacaaga agacgctcc acgcatcaa 
      aacgataat aataacact aataacgaa aatacgata 
      gggggcaca cagatgggt gtacaggaa attctgaat 
      aataacacg ggggagacc ctcacccct agttcgagt 
      acatcgccg gtttcgcca gtggtcagt ccacaaaaa 
      accacatca acgcctcaa acgaccgac aatcgaaac 
      aattttatt acgctatcg ccgacttcg acgtctgtt 
      attccagcg cctaataat atcagttgt aacgcaccg 
      aatggcgaa aatacgaac gttgtgagc ccacaaatg 
      gggcaaggg caaggccaa agggctatc gtgcgcagc 
      gaaaggcgg caaaggagt gcagaggag cgaagggat 
      gggagcagc aggaggagg tcagggagg aataacagg 
      aattcggga gggacgaca gcccaaggg ccggttaca 
      cagaacagc aagctggat ttgcctccc ggttacgag 
      ttgaggaca actcagcag ggacaggtc tatttctac 
      cacatccct accggcgta tcgacatgg cacgacccc 
      cgaataccc aaagacttg gcccccttg agcctggct 
      ttggaccat ttgggcccc cttccgcct ggttgggaa 
      atgcgccaa acagcctca ggccggatt tatttcgtt 
      gatcacaac aacaggacg actcaattc accgaccct 
      cgcctcaac actcaaata ctcaacaat attttacgt 
      cgggctagc gccacctca accacgacg ccaacaact 
      agaactacc ccaactacg acaacagca agtacaaca 
      tcgccttca acgtcaaca acaaccgtt acgacaaca 
      acgacaacg acagcagtg cctcaaaat ccggcacca 
      ctttcaccg ccgcctcgc actcggatt gtggaggag 
      ttgcctcaa agtctactc aacgattgc gatcattta 
      ccgaaatat cggcgggat ttagttgcg aaattgaag 
      actttacga gcggaatta acggcattg cagccgcaa 
      agtggtcat tgtagactt gaagtttcg cgtaatgaa 
      gtgtttgaa gagagttat agacttata atgaaaatg 
      cgaccgaaa gatatgcgc aaaagactg atggtgaag 
      tttaaaggg gaggaggga ttggattat gggggcgtg 
      gcacgcgag tggttgcat ttgttgtcg cgggaaatg 
      ttaaaccca caatatggg ttgtttcaa tacagtcgg 
      gatgatcac tacacgtta caaattaat ccggattcg 
      gcggtaaat ccggaacat ttgtcgtat tttcatttt 
      gttggaagg attttgggg attgcagtg tttcataat 
      catcagtta gagggcggg tttacgttg ccgttttat 
      aaacaattg cttaataaa ccaatcacg ttgcaagat 
      attgaaggg gtggatccg gaactacac cggagtctc 
      acgtggatg ttggaaaac aacatcgac ggcgtcctc 
      gacaccacc ttctcggtg gagaacaac agtttcgga 
      atcgtaaaa gtgcacgaa ttgaagcca agcggcgcc 
      accattcca gtaaccgaa gacaacaaa cgcgaatac 
      gtcaaactt tacgtaaat tatcgtttt atgcgcgga 
      attgagcaa caattcctt gcgcttcag aaaggtttc 
      actgaactt attccaccg tcactactt cgcccgttc 
      gacgaacgc gaactggaa ctagtgatt agtggaatc 
      ggttcgata gatatcgcc gattggcga agtcacaca 
      cgactcaaa cactgcaca cctgaaact cccgttgtt 
      caatggttt tggcaagtg gtggagtcg tactcggag 
      gagatgagg gcgaggttg ttgcagttc gttaccggg 
      tcgtcccgg gtgcctttg caggggttc aaggccctg 
      caggggagc acgggggcg gcgggacct cggctcttc 
      actatacat tgtatagat tgttcaccg cagaatttg 
      cctaaagca cacacttgt tttaatcga attgatatt 
      cctccctat gatagttat cagacgttg gccgataag 
      ttgacgcag gctgtggag gagacgtgc gggtttgcc 
      gtggagtga
    mRNA sequence
    • >TC006704 RA
      ctgtgggaa aaaaatatt cgtattcgg cgcccattt 
      tggtagatg tcgctcgcg tcgccccgt tcgaaaatg 
      gcgttgtgt gtgaaaagt gagtgatgt gttgacaaa 
      aaccagcaa tttgaaaat gtccaacgc ccccacgag 
      tcgtcgaaa tggtgccca aaaaatccg actgacgat 
      tctttgtgc gaggaattt ggtccggaa ggatttgtt 
      ccgccttcc cgacccttt tgcgaagat ttcggtgga 
      cggtagtgg acagtgcca ctccaccga gttcgttaa 
      aaacacgtt ggagcccaa gtggaactc gcactacga 
      cctgtacat cgggcgcaa tgattccat cacgatctc 
      ggtgtggaa ccaccgcaa agtcatcaa gaagcaggg 
      cagcgggtt cctgggctg cgtccggat tgttaattc 
      gcacatcca gaggctgag ggacaccgg ataccaatg 
      tttggatct gggaaaagc atgtccggc cgacagcga 
      accggtcaa aggtcaaat catcatttc gttattatc 
      acgcgatgg cccttgtag tggtacacc tttggccgt 
      tgttggccc ccaaggcga gttacgggg gccaactga 
      tcgcgattc ttcgccctc aaataacga cgatttacc 
      gcccggctg ggaggaacg ccgaacgga aaacggaag 
      gccctacta cgttaatca catcaccag gtccacaca 
      gtggattaa accacaatc gaccaacaa aacaagaag 
      acgctccac gcatcaaaa cgataataa taacactaa 
      taacgaaaa tacgatagg gggcacaca gatgggtgt 
      acaggaaat tctgaataa taacacggg ggagaccct 
      cacccctag ttcgagtac atcgccggt ttcgccagt 
      ggtcagtcc acaaaaaac cacatcaac gcctcaaac 
      gaccgacaa tcgaaacaa ttttattac gctatcgcc 
      gacttcgac gtctgttat tccagcgcc taataatat 
      cagttgtaa cgcaccgaa tggcgaaaa tacgaacgt 
      tgtgagccc acaaatggg gcaagggca aggccaaag 
      ggctatcgt gcgcagcga aaggcggca aaggagtgc 
      agaggagcg aagggatgg gagcagcag gaggaggtc 
      agggaggaa taacaggaa ttcgggagg gacgacagc 
      ccaagggcc ggttacaca gaacagcaa gctggattt 
      gcctcccgg ttacgagtt gaggacaac tcagcaggg 
      acaggtcta tttctacca catccctac cggcgtatc 
      gacatggca cgacccccg aatacccaa agacttggc 
      ccccttgag cctggcttt ggaccattt gggccccct 
      tccgcctgg ttgggaaat gcgccaaac agcctcagg 
      ccggattta tttcgttga tcacaacaa caggacgac 
      tcaattcac cgaccctcg cctcaacac tcaaatact 
      caacaatat tttacgtcg ggctagcgc cacctcaac 
      cacgacgcc aacaactag aactacccc aactacgac 
      aacagcaag tacaacatc gccttcaac gtcaacaac 
      aaccgttac gacaacaac gacaacgac agcagtgcc 
      tcaaaatcc ggcaccact ttcaccgcc gcctcgcac 
      tcggattgt ggaggagtt gcctcaaag tctactcaa 
      cgattgcga tcatttacc gaaatatcg gcgggattt 
      agttgcgaa attgaagac tttacgagc ggaattaac 
      ggcattgca gccgcaaag tggtcattg tagacttga 
      agtttcgcg taatgaagt gtttgaaga gagttatag 
      acttataat gaaaatgcg accgaaaga tatgcgcaa 
      aagactgat ggtgaagtt taaagggga ggagggatt 
      ggattatgg gggcgtggc acgcgagtg gttgcattt 
      gttgtcgcg ggaaatgtt aaacccaca atatgggtt 
      gtttcaata cagtcggga tgatcacta cacgttaca 
      aattaatcc ggattcggc ggtaaatcc ggaacattt 
      gtcgtattt tcattttgt tggaaggat tttggggat 
      tgcagtgtt tcataatca tcagttaga gggcgggtt 
      tacgttgcc gttttataa acaattgct taataaacc 
      aatcacgtt gcaagatat tgaaggggt ggatccgga 
      actacaccg gagtctcac gtggatgtt ggaaaacaa 
      catcgacgg cgtcctcga caccacctt ctcggtgga 
      gaacaacag tttcggaat cgtaaaagt gcacgaatt 
      gaagccaag cggcgccac cattccagt aaccgaaga 
      caacaaacg cgaatacgt caaacttta cgtaaatta 
      tcgttttat gcgcggaat tgagcaaca attccttgc 
      gcttcagaa aggtttcac tgaacttat tccaccgtc 
      actacttcg cccgttcga cgaacgcga actggaact 
      agtgattag tggaatcgg ttcgataga tatcgccga 
      ttggcgaag tcacacacg actcaaaca ctgcacacc 
      tgaaactcc cgttgttca atggttttg gcaagtggt 
      ggagtcgta ctcggagga gatgagggc gaggttgtt 
      gcagttcgt taccgggtc gtcccgggt gcctttgca 
      ggggttcaa ggccctgca ggggagcac gggggcggc 
      gggacctcg gctcttcac tatacattg tatagattg 
      ttcaccgca gaatttgcc taaagcaca cacttgttt 
      taatcgaat tgatattcc tccctatga tagttatca 
      gacgttggc cgataagtt gacgcaggc tgtggagga 
      gacgtgcgg gtttgccgt ggagtgata agaaatcgt 
      ccgcatatc atttggtct tttgcaaga ctgaagagt 
      gccttgctc tcacggtta cggttcaaa agtaagtgc 
      ctaagctag tcatttcac gctcgtagc tcgcttcaa 
      aaccaaatt cgctttccg attaaacca acactttgc 
      ctaaatgtg acatattgg tgatagaaa atgcctaag 
      cgatggcca aaaagtgct ttaaatcaa caataaaga 
      ttctattgt agctgttgt taattgatg atgtttatc 
      tcggtaaaa agacaaatc tatttttat gtaatttcc 
      tcggaggaa tggagtatt tgttttgtg attgtttct 
      ctgtaaata ttgcatacg aactgatta tatgattag 
      gttaatgat ttgataagc ggtattgtg attttttct 
      ataatttat cgacttgta catagtttt aacttttgc 
      accttttgt tatctactt tgtacattt ttttctatt 
      tctaaagta aggatacac aaagcaata ttgtaacgt 
      tatagtgat acgagtttt taaccgcac ttgattata 
      tcacgtaat gaaacgttt cgtaatgat attttttaa 
      gttactgtt taattcttt catgatcac tacaagaat 
      actccacga tgtacataa gctggctgg ttttgttcc 
      ctttctcaa tacgtctgt tcattttag tgataattt 
      aattgtggt gtttatgtt aagatttat ttgtatatt 
      gcattcaga tgaatatat tttaaacta tttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY