Status
Please wait ...
Gene information
General information
TC006640 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12255571..12259007
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006640
    iBeetle screen iB_02879

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006640 RA
      MCSKFSALA LLCAASCVL GAEVKDKRQ 
      VGSVTLQKD DHQVAETHG FGAPAAVHA 
      GSKYFQYAH VPAKDHYEH GHKRGNEHH 
      FIERHEKAH PHAGEFKTK VRWGDKHGG 
      YGEHYWDYN HAGHHDGGG EESQQKEEY 
      VAYDESQQS PTYVAGGRG KRQPNVDGD 
      VEFINENAR ALVLSGVTG KHGGNYKRE 
      RDYERGKRE GKKLDGRDL VYDQKMGLI 
      VDQKTGIAY ELKPVVEY
    CDS sequence
    • >TC006640 RA
      atgtgctca aagttttca gcgttggct ttgctctgc 
      gccgcctcc tgcgtcctg ggggcggaa gtgaaggac 
      aaaagacaa gttggaagc gtcacgttg caaaaggac 
      gaccaccaa gtggcggag acgcacggc ttcggcgcc 
      ccggcggcc gtacatgcg ggcagcaaa tatttccaa 
      tacgcccat gtgcccgct aaagaccat tacgagcac 
      ggccacaag cgaggcaac gaacaccac tttatcgag 
      cgtcatgag aaagcccat ccgcatgcc ggagagttc 
      aaaactaaa gtgagatgg ggtgacaag cacgggggg 
      tacggggag cactattgg gactacaac catgctggt 
      catcatgat ggcggtggc gaggagagc cagcagaag 
      gaggaatat gtggcgtac gacgagagc cagcagagt 
      ccgacctat gtggctgga gggagaggc aaaaggcag 
      ccgaatgtg gacggagat gtggagttt attaatgaa 
      aatgccagg gctttggtt ctgagtgga gtaacaggc 
      aaacacggc gggaattac aagagggaa agggattac 
      gagcggggc aagcgagaa gggaagaag ttagacggc 
      agggacttg gtctacgac cagaagatg ggcctgatt 
      gttgaccag aaaactggg attgcgtac gagttgaag 
      ccggttgtt gaatattaa
    mRNA sequence
    • >TC006640 RA
      tcttgcaaa atgtgctca aagttttca gcgttggct 
      ttgctctgc gccgcctcc tgcgtcctg ggggcggaa 
      gtgaaggac aaaagacaa gttggaagc gtcacgttg 
      caaaaggac gaccaccaa gtggcggag acgcacggc 
      ttcggcgcc ccggcggcc gtacatgcg ggcagcaaa 
      tatttccaa tacgcccat gtgcccgct aaagaccat 
      tacgagcac ggccacaag cgaggcaac gaacaccac 
      tttatcgag cgtcatgag aaagcccat ccgcatgcc 
      ggagagttc aaaactaaa gtgagatgg ggtgacaag 
      cacgggggg tacggggag cactattgg gactacaac 
      catgctggt catcatgat ggcggtggc gaggagagc 
      cagcagaag gaggaatat gtggcgtac gacgagagc 
      cagcagagt ccgacctat gtggctgga gggagaggc 
      aaaaggcag ccgaatgtg gacggagat gtggagttt 
      attaatgaa aatgccagg gctttggtt ctgagtgga 
      gtaacaggc aaacacggc gggaattac aagagggaa 
      agggattac gagcggggc aagcgagaa gggaagaag 
      ttagacggc agggacttg gtctacgac cagaagatg 
      ggcctgatt gttgaccag aaaactggg attgcgtac 
      gagttgaag ccggttgtt gaatattaa gtgtcttta 
      tagtttttg tacgtttgt tgactagtt tttaataaa 
      tacttttac gaaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY