| General information | |||
| TC010211 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 15656078..15669691 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC010211 | ||
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02878 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC010211 RA
MSNSFQEVL LGFYVAHKN RESFLRLHN YDTVGDLKS EIFKKLNVP PTRQIIDCW STNPESDND ILGACCDYH TNYLVLYDS IDLREDDIV MTEETINNF KELEHLAAI EFIDYFSTN FDDKGITFH ACCLEDAIR EACFTSIEK RNVLLLYLH NKNDTFAKI FSENLVTPE VKDVISKRF FLLGWNLEE ESYHEALIE ALSNRSELS ALVNFVKSK TCAALLIVP IKNSFTLFS CMKNKVSKK DFLETLKRA KEFLVTEND IEKDLEKIE DSVNDFGSV NYQQLMLDG LGDRDYDNY DHFEIHLLK QKIGYALFG PPVEENGYD KKQMKKINS LSEVIVNQN NRFAKWTDR VILSIIYNC TEPLPSVKQ KRKKNTKYE DYNPNTDLT PVPIFILRK CKNSDNPCR VIIDDGGRV YRTWNSYLT ENKLPECEM ILPLNGRYQ ADERGRVIL ERHLSPVCG VGSKILQGT DIANTAAGI ISGGIFIAA AIPAITVAP VALVAAGAT GLGVGLYSL GRSAYTLYD RKAHKETIS FANSEARGA YLNIVAGSL GFVGAGANI AISQLAAKG FNIGQGATV TVNMINVAN IGASGISVA NSGYDVFDQ WWSENQTPS MLTIIQLSS SILFFGHAV YNFKSVGAM VEESQARTL KDYQDSLRS NRHRKTFNK MMKETIRQN NGNVQKGQA EVIKTVINI PNKDEVFAT LTRLNKQMN KNNVKFSAN NGDILLNGV SIDIAKFNT MDSNEVATF LTTLPATPE PSPDQIQAM SSKLQLSFR KVNKQEIAG LAIKILRMF SKTDENIKE KIINAVAAV ISRLIKETP GCFAEFTRM FPGLDKYLR LVSMVNSHF QQLIDAMEE QYKKWLATK NPDIEEPIF VKLAFDYSR RVVQLFDFV TKAYFMGNN ITEVGLREL IKYFYTWFA KQAIEYDEY MTRKQERRI HSGTPKVEM QCPDCGGVY YLMIIR
- >TC010211 RA
atgtccaac tcatttcag gaggtgtta ctagggttt tatgtcgct cataagaac cgtgaaagt tttcttcgg ttgcacaat tatgatacc gtcggtgat ttgaaaagt gagattttt aaaaaactg aatgtgcct cctacgaga caaatcatc gattgttgg tccacaaat ccggagagt gataatgat attttaggt gcctgttgt gattatcac acaaattat ttagttttg tatgactca attgattta cgtgaagac gacatagta atgacggaa gaaacaata aataatttc aaagaatta gagcattta gctgcgatt gagtttatt gattatttt tcaactaat tttgacgat aaaggaata acgttccat gcgtgttgt ctggaagat gccataaga gaagcttgt ttcacgtct atagaaaag cgaaatgtc ttactactt tacctccac aataaaaat gacactttt gcaaaaata ttttctgaa aatttagtc acaccggag gtgaaggat gtaataagt aaacgattt ttccttctt ggttggaat ttagaagaa gaaagctac catgaagcg ttaattgaa gctttaagt aatcgcagt gaattatca gctcttgtg aatttcgta aaatcgaag acttgcgcg gccttacta atagtgcct atcaaaaat tctttcact ttattttca tgtatgaaa aataaagtg tcaaagaaa gattttttg gaaacttta aaacgagct aaagagttc ctagtcaca gaaaatgat attgagaag gatcttgag aaaatcgaa gactctgtg aacgatttt ggttcagta aattatcaa caactcatg ttggatggc cttggagac cgtgattac gacaattac gaccatttc gaaatccat ttattaaaa cagaaaatt ggttatgct ttatttgga cctcctgtt gaagaaaac ggttatgac aagaaacaa atgaaaaaa attaattcc ttaagcgaa gttattgta aaccaaaac aatcgtttc gcaaaatgg acagacaga gttatactc tccataatt tacaactgt actgaaccc cttccaagt gttaaacaa aaacgtaag aaaaatacg aaatacgaa gattacaac cccaacacc gacctcact cccgttcca attttcatc ctacgaaaa tgcaaaaac agcgacaat ccctgtcgc gttataatc gacgacggt ggtcgggtc tacagaacc tggaactcc tacttgacg gaaaacaaa ttgcctgaa tgtgaaatg attcttcca ctcaatggc cgttatcaa gccgacgaa cgtggcaga gttattctc gagcgtcac ctatcacct gtctgcggc gtaggctcg aagattctt caaggtact gacatagca aacacggcc gcaggaatt atttcagga gggattttt atcgctgct gctattccg gccatcact gtagcccct gtagctttg gtggcggca ggagccacg ggactaggc gtggggctg tattccttg ggtcgcagc gcctacaca ctttacgac agaaaggca cataaggag actataagt tttgctaat tcagaagcc agaggagcg tacttaaac atcgtggca ggttctttg ggatttgtg ggggcagga gccaacatc gcaatcagt caacttgcc gccaagggt ttcaacatt ggccagggg gccacagtg accgtcaac atgataaac gtggcaaat attggtgct agtggcatc agcgttgcc aattcaggt tatgatgtc ttcgaccag tggtggagc gagaaccag acgccatct atgttgacg ataattcaa ctcagttcg tcgattctg ttcttcggc cacgctgtg tataatttc aaatcggtg ggggccatg gttgaagag tctcaagcg cgcactttg aaagactat caagactct ttgcgcagc aatcggcat agaaagact ttcaataaa atgatgaaa gaaactata agacagaac aatggtaat gtccaaaaa ggtcaagcc gaagtgatt aaaaccgtt atcaatatc ccgaacaag gatgaagtt tttgctact ttgaccaga cttaataaa caaatgaat aaaaacaac gtcaaattt tcggccaac aatggcgac attttgtta aatggcgtc agtatagat atagcgaaa ttcaatact atggattcg aatgaagtg gcgacattt ttaacaact cttcctgcc acaccagag ccatctcca gaccaaatc caagcgatg agctctaaa ctacaactc tctttccgt aaagtgaac aagcaagaa atcgccggt ttggccatt aaaattttg cggatgttt agtaaaacc gacgaaaat atcaaagag aaaataatc aacgctgtt gcggccgtt atttcgagg ttgataaag gaaacgcca ggttgcttt gccgaattc actcgcatg tttcccggc ctggacaaa tacctaaga ctggtgtcg atggtcaat agtcatttc caacaactg atcgatgcg atggaagaa cagtacaag aagtggttg gctaccaaa aatccagat attgaagaa ccgattttt gtcaaattg gcgttcgat tattcgaga cgggttgtt caattattt gattttgtt accaaagct tactttatg ggaaacaat attaccgaa gtcggatta agagaatta attaagtat ttttatacc tggtttgcg aaacaagca atcgagtat gatgaatat atgacgagg aaacaggaa agaaggatt cacagtgga acgcctaaa gtagaaatg cagtgtcct gattgtggc ggagtttat tatctaatg ataattaga taa
- >TC010211 RA
ttatttgtg attccaacg tatttatga caatgtcca actcatttc aggaggtgt tactagggt tttatgtcg ctcataaga accgtgaaa gttttcttc ggttgcaca attatgata ccgtcggtg atttgaaaa gtgagattt ttaaaaaac tgaatgtgc ctcctacga gacaaatca tcgattgtt ggtccacaa atccggaga gtgataatg atattttag gtgcctgtt gtgattatc acacaaatt atttagttt tgtatgact caattgatt tacgtgaag acgacatag taatgacgg aagaaacaa taaataatt tcaaagaat tagagcatt tagctgcga ttgagttta ttgattatt tttcaacta attttgacg ataaaggaa taacgttcc atgcgtgtt gtctggaag atgccataa gagaagctt gtttcacgt ctatagaaa agcgaaatg tcttactac tttacctcc acaataaaa atgacactt ttgcaaaaa tattttctg aaaatttag tcacaccgg aggtgaagg atgtaataa gtaaacgat ttttccttc ttggttgga atttagaag aagaaagct accatgaag cgttaattg aagctttaa gtaatcgca gtgaattat cagctcttg tgaatttcg taaaatcga agacttgcg cggccttac taatagtgc ctatcaaaa attctttca ctttatttt catgtatga aaaataaag tgtcaaaga aagattttt tggaaactt taaaacgag ctaaagagt tcctagtca cagaaaatg atattgaga aggatcttg agaaaatcg aagactctg tgaacgatt ttggttcag taaattatc aacaactca tgttggatg gccttggag accgtgatt acgacaatt acgaccatt tcgaaatcc atttattaa aacagaaaa ttggttatg ctttatttg gacctcctg ttgaagaaa acggttatg acaagaaac aaatgaaaa aaattaatt ccttaagcg aagttattg taaaccaaa acaatcgtt tcgcaaaat ggacagaca gagttatac tctccataa tttacaact gtactgaac cccttccaa gtgttaaac aaaaacgta agaaaaata cgaaatacg aagattaca accccaaca ccgacctca ctcccgttc caattttca tcctacgaa aatgcaaaa acagcgaca atccctgtc gcgttataa tcgacgacg gtggtcggg tctacagaa cctggaact cctacttga cggaaaaca aattgcctg aatgtgaaa tgattcttc cactcaatg gccgttatc aagccgacg aacgtggca gagttattc tcgagcgtc acctatcac ctgtctgcg gcgtaggct cgaagattc ttcaaggta ctgacatag caaacacgg ccgcaggaa ttatttcag gagggattt ttatcgctg ctgctattc cggccatca ctgtagccc ctgtagctt tggtggcgg caggagcca cgggactag gcgtggggc tgtattcct tgggtcgca gcgcctaca cactttacg acagaaagg cacataagg agactataa gttttgcta attcagaag ccagaggag cgtacttaa acatcgtgg caggttctt tgggatttg tgggggcag gagccaaca tcgcaatca gtcaacttg ccgccaagg gtttcaaca ttggccagg gggccacag tgaccgtca acatgataa acgtggcaa atattggtg ctagtggca tcagcgttg ccaattcag gttatgatg tcttcgacc agtggtgga gcgagaacc agacgccat ctatgttga cgataattc aactcagtt cgtcgattc tgttcttcg gccacgctg tgtataatt tcaaatcgg tgggggcca tggttgaag agtctcaag cgcgcactt tgaaagact atcaagact ctttgcgca gcaatcggc atagaaaga ctttcaata aaatgatga aagaaacta taagacaga acaatggta atgtccaaa aaggtcaag ccgaagtga ttaaaaccg ttatcaata tcccgaaca aggatgaag tttttgcta ctttgacca gacttaata aacaaatga ataaaaaca acgtcaaat tttcggcca acaatggcg acattttgt taaatggcg tcagtatag atatagcga aattcaata ctatggatt cgaatgaag tggcgacat ttttaacaa ctcttcctg ccacaccag agccatctc cagaccaaa tccaagcga tgagctcta aactacaac tctctttcc gtaaagtga acaagcaag aaatcgccg gtttggcca ttaaaattt tgcggatgt ttagtaaaa ccgacgaaa atatcaaag agaaaataa tcaacgctg ttgcggccg ttatttcga ggttgataa aggaaacgc caggttgct ttgccgaat tcactcgca tgtttcccg gcctggaca aatacctaa gactggtgt cgatggtca atagtcatt tccaacaac tgatcgatg cgatggaag aacagtaca agaagtggt tggctacca aaaatccag atattgaag aaccgattt ttgtcaaat tggcgttcg attattcga gacgggttg ttcaattat ttgattttg ttaccaaag cttacttta tgggaaaca atattaccg aagtcggat taagagaat taattaagt atttttata cctggtttg cgaaacaag caatcgagt atgatgaat atatgacga ggaaacagg aaagaagga ttcacagtg gaacgccta aagtagaaa tgcagtgtc ctgattgtg gcggagttt attatctaa tgataatta gataaaatt aatattttg tcggaggct tttcaaaac acacaaatc aaacatatc caaagagtt agaatttat agtggttac accatataa catatagtg attgaagaa tatttttag aaatgaatt ttgcaacga gatttccac tttcagata ggtgctgtt atctaaatg gaaatttaa gtttttcgt aaaaaaatt atttgttat tttttaaat gtatttatt aaattgtgc actaaataa atgttaatt ctttta
