Status
Please wait ...
Gene information
General information
TC010211 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 15656078..15669691
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010211
    iBeetle screen iB_02878

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010211 RA
      MSNSFQEVL LGFYVAHKN RESFLRLHN YDTVGDLKS 
      EIFKKLNVP PTRQIIDCW STNPESDND ILGACCDYH 
      TNYLVLYDS IDLREDDIV MTEETINNF KELEHLAAI 
      EFIDYFSTN FDDKGITFH ACCLEDAIR EACFTSIEK 
      RNVLLLYLH NKNDTFAKI FSENLVTPE VKDVISKRF 
      FLLGWNLEE ESYHEALIE ALSNRSELS ALVNFVKSK 
      TCAALLIVP IKNSFTLFS CMKNKVSKK DFLETLKRA 
      KEFLVTEND IEKDLEKIE DSVNDFGSV NYQQLMLDG 
      LGDRDYDNY DHFEIHLLK QKIGYALFG PPVEENGYD 
      KKQMKKINS LSEVIVNQN NRFAKWTDR VILSIIYNC 
      TEPLPSVKQ KRKKNTKYE DYNPNTDLT PVPIFILRK 
      CKNSDNPCR VIIDDGGRV YRTWNSYLT ENKLPECEM 
      ILPLNGRYQ ADERGRVIL ERHLSPVCG VGSKILQGT 
      DIANTAAGI ISGGIFIAA AIPAITVAP VALVAAGAT 
      GLGVGLYSL GRSAYTLYD RKAHKETIS FANSEARGA 
      YLNIVAGSL GFVGAGANI AISQLAAKG FNIGQGATV 
      TVNMINVAN IGASGISVA NSGYDVFDQ WWSENQTPS 
      MLTIIQLSS SILFFGHAV YNFKSVGAM VEESQARTL 
      KDYQDSLRS NRHRKTFNK MMKETIRQN NGNVQKGQA 
      EVIKTVINI PNKDEVFAT LTRLNKQMN KNNVKFSAN 
      NGDILLNGV SIDIAKFNT MDSNEVATF LTTLPATPE 
      PSPDQIQAM SSKLQLSFR KVNKQEIAG LAIKILRMF 
      SKTDENIKE KIINAVAAV ISRLIKETP GCFAEFTRM 
      FPGLDKYLR LVSMVNSHF QQLIDAMEE QYKKWLATK 
      NPDIEEPIF VKLAFDYSR RVVQLFDFV TKAYFMGNN 
      ITEVGLREL IKYFYTWFA KQAIEYDEY MTRKQERRI 
      HSGTPKVEM QCPDCGGVY YLMIIR
    CDS sequence
    • >TC010211 RA
      atgtccaac tcatttcag gaggtgtta ctagggttt 
      tatgtcgct cataagaac cgtgaaagt tttcttcgg 
      ttgcacaat tatgatacc gtcggtgat ttgaaaagt 
      gagattttt aaaaaactg aatgtgcct cctacgaga 
      caaatcatc gattgttgg tccacaaat ccggagagt 
      gataatgat attttaggt gcctgttgt gattatcac 
      acaaattat ttagttttg tatgactca attgattta 
      cgtgaagac gacatagta atgacggaa gaaacaata 
      aataatttc aaagaatta gagcattta gctgcgatt 
      gagtttatt gattatttt tcaactaat tttgacgat 
      aaaggaata acgttccat gcgtgttgt ctggaagat 
      gccataaga gaagcttgt ttcacgtct atagaaaag 
      cgaaatgtc ttactactt tacctccac aataaaaat 
      gacactttt gcaaaaata ttttctgaa aatttagtc 
      acaccggag gtgaaggat gtaataagt aaacgattt 
      ttccttctt ggttggaat ttagaagaa gaaagctac 
      catgaagcg ttaattgaa gctttaagt aatcgcagt 
      gaattatca gctcttgtg aatttcgta aaatcgaag 
      acttgcgcg gccttacta atagtgcct atcaaaaat 
      tctttcact ttattttca tgtatgaaa aataaagtg 
      tcaaagaaa gattttttg gaaacttta aaacgagct 
      aaagagttc ctagtcaca gaaaatgat attgagaag 
      gatcttgag aaaatcgaa gactctgtg aacgatttt 
      ggttcagta aattatcaa caactcatg ttggatggc 
      cttggagac cgtgattac gacaattac gaccatttc 
      gaaatccat ttattaaaa cagaaaatt ggttatgct 
      ttatttgga cctcctgtt gaagaaaac ggttatgac 
      aagaaacaa atgaaaaaa attaattcc ttaagcgaa 
      gttattgta aaccaaaac aatcgtttc gcaaaatgg 
      acagacaga gttatactc tccataatt tacaactgt 
      actgaaccc cttccaagt gttaaacaa aaacgtaag 
      aaaaatacg aaatacgaa gattacaac cccaacacc 
      gacctcact cccgttcca attttcatc ctacgaaaa 
      tgcaaaaac agcgacaat ccctgtcgc gttataatc 
      gacgacggt ggtcgggtc tacagaacc tggaactcc 
      tacttgacg gaaaacaaa ttgcctgaa tgtgaaatg 
      attcttcca ctcaatggc cgttatcaa gccgacgaa 
      cgtggcaga gttattctc gagcgtcac ctatcacct 
      gtctgcggc gtaggctcg aagattctt caaggtact 
      gacatagca aacacggcc gcaggaatt atttcagga 
      gggattttt atcgctgct gctattccg gccatcact 
      gtagcccct gtagctttg gtggcggca ggagccacg 
      ggactaggc gtggggctg tattccttg ggtcgcagc 
      gcctacaca ctttacgac agaaaggca cataaggag 
      actataagt tttgctaat tcagaagcc agaggagcg 
      tacttaaac atcgtggca ggttctttg ggatttgtg 
      ggggcagga gccaacatc gcaatcagt caacttgcc 
      gccaagggt ttcaacatt ggccagggg gccacagtg 
      accgtcaac atgataaac gtggcaaat attggtgct 
      agtggcatc agcgttgcc aattcaggt tatgatgtc 
      ttcgaccag tggtggagc gagaaccag acgccatct 
      atgttgacg ataattcaa ctcagttcg tcgattctg 
      ttcttcggc cacgctgtg tataatttc aaatcggtg 
      ggggccatg gttgaagag tctcaagcg cgcactttg 
      aaagactat caagactct ttgcgcagc aatcggcat 
      agaaagact ttcaataaa atgatgaaa gaaactata 
      agacagaac aatggtaat gtccaaaaa ggtcaagcc 
      gaagtgatt aaaaccgtt atcaatatc ccgaacaag 
      gatgaagtt tttgctact ttgaccaga cttaataaa 
      caaatgaat aaaaacaac gtcaaattt tcggccaac 
      aatggcgac attttgtta aatggcgtc agtatagat 
      atagcgaaa ttcaatact atggattcg aatgaagtg 
      gcgacattt ttaacaact cttcctgcc acaccagag 
      ccatctcca gaccaaatc caagcgatg agctctaaa 
      ctacaactc tctttccgt aaagtgaac aagcaagaa 
      atcgccggt ttggccatt aaaattttg cggatgttt 
      agtaaaacc gacgaaaat atcaaagag aaaataatc 
      aacgctgtt gcggccgtt atttcgagg ttgataaag 
      gaaacgcca ggttgcttt gccgaattc actcgcatg 
      tttcccggc ctggacaaa tacctaaga ctggtgtcg 
      atggtcaat agtcatttc caacaactg atcgatgcg 
      atggaagaa cagtacaag aagtggttg gctaccaaa 
      aatccagat attgaagaa ccgattttt gtcaaattg 
      gcgttcgat tattcgaga cgggttgtt caattattt 
      gattttgtt accaaagct tactttatg ggaaacaat 
      attaccgaa gtcggatta agagaatta attaagtat 
      ttttatacc tggtttgcg aaacaagca atcgagtat 
      gatgaatat atgacgagg aaacaggaa agaaggatt 
      cacagtgga acgcctaaa gtagaaatg cagtgtcct 
      gattgtggc ggagtttat tatctaatg ataattaga 
      taa
    mRNA sequence
    • >TC010211 RA
      ttatttgtg attccaacg tatttatga caatgtcca 
      actcatttc aggaggtgt tactagggt tttatgtcg 
      ctcataaga accgtgaaa gttttcttc ggttgcaca 
      attatgata ccgtcggtg atttgaaaa gtgagattt 
      ttaaaaaac tgaatgtgc ctcctacga gacaaatca 
      tcgattgtt ggtccacaa atccggaga gtgataatg 
      atattttag gtgcctgtt gtgattatc acacaaatt 
      atttagttt tgtatgact caattgatt tacgtgaag 
      acgacatag taatgacgg aagaaacaa taaataatt 
      tcaaagaat tagagcatt tagctgcga ttgagttta 
      ttgattatt tttcaacta attttgacg ataaaggaa 
      taacgttcc atgcgtgtt gtctggaag atgccataa 
      gagaagctt gtttcacgt ctatagaaa agcgaaatg 
      tcttactac tttacctcc acaataaaa atgacactt 
      ttgcaaaaa tattttctg aaaatttag tcacaccgg 
      aggtgaagg atgtaataa gtaaacgat ttttccttc 
      ttggttgga atttagaag aagaaagct accatgaag 
      cgttaattg aagctttaa gtaatcgca gtgaattat 
      cagctcttg tgaatttcg taaaatcga agacttgcg 
      cggccttac taatagtgc ctatcaaaa attctttca 
      ctttatttt catgtatga aaaataaag tgtcaaaga 
      aagattttt tggaaactt taaaacgag ctaaagagt 
      tcctagtca cagaaaatg atattgaga aggatcttg 
      agaaaatcg aagactctg tgaacgatt ttggttcag 
      taaattatc aacaactca tgttggatg gccttggag 
      accgtgatt acgacaatt acgaccatt tcgaaatcc 
      atttattaa aacagaaaa ttggttatg ctttatttg 
      gacctcctg ttgaagaaa acggttatg acaagaaac 
      aaatgaaaa aaattaatt ccttaagcg aagttattg 
      taaaccaaa acaatcgtt tcgcaaaat ggacagaca 
      gagttatac tctccataa tttacaact gtactgaac 
      cccttccaa gtgttaaac aaaaacgta agaaaaata 
      cgaaatacg aagattaca accccaaca ccgacctca 
      ctcccgttc caattttca tcctacgaa aatgcaaaa 
      acagcgaca atccctgtc gcgttataa tcgacgacg 
      gtggtcggg tctacagaa cctggaact cctacttga 
      cggaaaaca aattgcctg aatgtgaaa tgattcttc 
      cactcaatg gccgttatc aagccgacg aacgtggca 
      gagttattc tcgagcgtc acctatcac ctgtctgcg 
      gcgtaggct cgaagattc ttcaaggta ctgacatag 
      caaacacgg ccgcaggaa ttatttcag gagggattt 
      ttatcgctg ctgctattc cggccatca ctgtagccc 
      ctgtagctt tggtggcgg caggagcca cgggactag 
      gcgtggggc tgtattcct tgggtcgca gcgcctaca 
      cactttacg acagaaagg cacataagg agactataa 
      gttttgcta attcagaag ccagaggag cgtacttaa 
      acatcgtgg caggttctt tgggatttg tgggggcag 
      gagccaaca tcgcaatca gtcaacttg ccgccaagg 
      gtttcaaca ttggccagg gggccacag tgaccgtca 
      acatgataa acgtggcaa atattggtg ctagtggca 
      tcagcgttg ccaattcag gttatgatg tcttcgacc 
      agtggtgga gcgagaacc agacgccat ctatgttga 
      cgataattc aactcagtt cgtcgattc tgttcttcg 
      gccacgctg tgtataatt tcaaatcgg tgggggcca 
      tggttgaag agtctcaag cgcgcactt tgaaagact 
      atcaagact ctttgcgca gcaatcggc atagaaaga 
      ctttcaata aaatgatga aagaaacta taagacaga 
      acaatggta atgtccaaa aaggtcaag ccgaagtga 
      ttaaaaccg ttatcaata tcccgaaca aggatgaag 
      tttttgcta ctttgacca gacttaata aacaaatga 
      ataaaaaca acgtcaaat tttcggcca acaatggcg 
      acattttgt taaatggcg tcagtatag atatagcga 
      aattcaata ctatggatt cgaatgaag tggcgacat 
      ttttaacaa ctcttcctg ccacaccag agccatctc 
      cagaccaaa tccaagcga tgagctcta aactacaac 
      tctctttcc gtaaagtga acaagcaag aaatcgccg 
      gtttggcca ttaaaattt tgcggatgt ttagtaaaa 
      ccgacgaaa atatcaaag agaaaataa tcaacgctg 
      ttgcggccg ttatttcga ggttgataa aggaaacgc 
      caggttgct ttgccgaat tcactcgca tgtttcccg 
      gcctggaca aatacctaa gactggtgt cgatggtca 
      atagtcatt tccaacaac tgatcgatg cgatggaag 
      aacagtaca agaagtggt tggctacca aaaatccag 
      atattgaag aaccgattt ttgtcaaat tggcgttcg 
      attattcga gacgggttg ttcaattat ttgattttg 
      ttaccaaag cttacttta tgggaaaca atattaccg 
      aagtcggat taagagaat taattaagt atttttata 
      cctggtttg cgaaacaag caatcgagt atgatgaat 
      atatgacga ggaaacagg aaagaagga ttcacagtg 
      gaacgccta aagtagaaa tgcagtgtc ctgattgtg 
      gcggagttt attatctaa tgataatta gataaaatt 
      aatattttg tcggaggct tttcaaaac acacaaatc 
      aaacatatc caaagagtt agaatttat agtggttac 
      accatataa catatagtg attgaagaa tatttttag 
      aaatgaatt ttgcaacga gatttccac tttcagata 
      ggtgctgtt atctaaatg gaaatttaa gtttttcgt 
      aaaaaaatt atttgttat tttttaaat gtatttatt 
      aaattgtgc actaaataa atgttaatt ctttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY