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Gene information
General information
TC010210 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid50: 228279..246560
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010210
    iBeetle screen iB_02877
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010210 RA
      MNAGETIVN DLNKESLDY IEKGLQSFI EFQETFRDL 
      CKDITRVIN EYTATGRLP AFDHLTDFE EYKKLRSRI 
      SNEEGSVSS KENHEPDDI PTTKKKARR SRRTAFKEA 
      TQSISGLNE RRHRTTLKK EKISMISGE ESVPEKISV 
      KTELEEMPA PTRPAPKKR RKNTTSKQI KQEKIEDPL 
      ENPRESDVI IEEAVVPLV NLVDSLEEE TNKTEVESS 
      KRTRTASDD GQMENKEKR PKSSEDETQ FEDAVSTVL 
      SENADKNNA TFVTASKLE VQGGNLNST VVVQDPKYL 
      KSLIPKTVD DLMTDDESE EEKETLKKS KVQGTIPKR 
      AKQIFSPFE QSPVKKKVQ AFEKLQEEA AAMPVRITR 
      TKTRNQAKE QDSAAEEPN QTEKVKSNV KEKAKIFTP 
      VVSKFLPKA ASTTVKNKN LKNSSSTEN LMIKSASAL 
      KASHAEYRE IEMRRHEKE QEALKKREA MLQAQSEEK 
      RRKREEKQL KAQQQREMI EKEKQKALE AQRLKEERH 
      RQHIAEQEE KKLKLKEEL EKKRLIVKN RAIEQKKYE 
      EEQKREEER KAAELAAKL AKQDEDITK MLQKQKERK 
      APIYMREPA PPLPSDDCH DSDSEEYKN QKVILKNWE 
      KEVNVMLMQ KYESAIGHA IKNTFFSLQ ARTPDLQEI 
      LEHIEPRKL KRTSSALWD QPPRFTMIP GLETHDETE 
      EFI
    CDS sequence
    • >TC010210 RA
      atgaacgcc ggtgaaact atagtaaac gacttgaat 
      aaagagtct ctcgattat atcgaaaag ggactgcag 
      tcctttatc gagttccaa gagacgttt cgagatcta 
      tgtaaggat attactagg gttattaac gagtacacc 
      gctacagga aggttaccg gcttttgat catctgact 
      gatttcgaa gagtataaa aagctgagg tcacgtatt 
      tcaaatgag gaaggatca gtatcatct aaagaaaac 
      cacgaacct gatgatatc cccacaaca aagaagaaa 
      gctcgccgt tctcggcga actgcattt aaagaagct 
      actcaaagc atcagcggt ctcaacgaa agacgtcac 
      cgcaccacc ctcaaaaag gagaaaata tcaatgatc 
      agtggtgaa gaatcggtg cctgagaaa atttcagtt 
      aagaccgaa cttgaagaa atgcctgct ccaactcga 
      ccagctcct aaaaagaga agaaaaaat actacaagt 
      aaacaaatc aaacaagaa aaaatagaa gatccatta 
      gagaatccg cgtgaatca gacgtcatc attgaagaa 
      gctgtggtt cctttggtt aatcttgtg gactcgttg 
      gaagaggaa accaataaa acagaagtg gagtcttca 
      aagcgaaca agaactgcg tccgatgat ggacaaatg 
      gaaaacaaa gaaaaaaga ccaaaatcc agtgaagat 
      gaaactcag tttgaagac gctgtttca actgtgttg 
      tcggaaaat gcagacaaa aataacgca acttttgtc 
      accgcttct aagcttgag gtccagggt ggtaattta 
      aattcaaca gtggttgta caagatccg aaatatctc 
      aagagcctg attccaaag accgtagat gatctgatg 
      accgacgat gaatcagaa gaagaaaaa gaaacatta 
      aagaaatcg aaggtccaa gggactatt cccaagcgc 
      gctaaacaa attttcagt ccgtttgaa caaagccct 
      gtgaagaaa aaggtccag gcttttgaa aaacttcaa 
      gaggaagcc gctgccatg cctgttcga atcacccga 
      actaaaaca agaaatcaa gcgaaagaa caagattcc 
      gcagcggaa gaaccgaat cagaccgaa aaggtgaaa 
      agtaacgtc aaagagaaa gcgaaaata tttactcca 
      gtcgtttcg aaatttcta ccaaaagcc gccagcaca 
      accgtaaaa aacaagaac ctaaagaac tcgagttcc 
      accgaaaac ctgatgatt aaaagtgca agcgcttta 
      aaagcatct cacgccgaa tatcgagaa attgagatg 
      cgccgtcac gaaaaggaa caagaagca ctgaaaaaa 
      cgcgaagcg atgctccaa gcgcaaagt gaagaaaaa 
      agacgtaaa agagaagaa aaacaactc aaggcgcaa 
      cagcaacgt gaaatgatt gaaaaagaa aaacagaaa 
      gctttagaa gcacaacgg ttgaaagag gaacgccat 
      cgacaacat atcgccgaa caagaagaa aagaaactg 
      aaattgaaa gaagaactg gaaaagaag agattgatc 
      gtgaaaaac cgagcgatt gaacagaag aaatacgag 
      gaagaacag aagagggaa gaggaaaga aaagctgca 
      gaattggct gcaaaattg gcaaagcag gacgaagat 
      attacaaag atgcttcag aagcaaaag gaaagaaag 
      gcaccgatt tatatgaga gagccggca ccgcctttg 
      ccgtcagat gactgccat gattcagac agtgaagaa 
      tataaaaat caaaaagtc atactaaaa aactgggaa 
      aaagaagtg aatgtaatg cttatgcaa aagtatgaa 
      tcggcgata ggtcatgca attaaaaac acattcttc 
      tcacttcaa gcacgaacg ccggatcta caagagatt 
      ttggaacat atcgaaccg agaaaattg aaaagaaca 
      tcgagtgct ctctgggac cagccgcca cgattcact 
      atgattccg gggttagag actcatgat gagacagag 
      gaatttatt taa
    mRNA sequence
    • >TC010210 RA
      aatagtgag aaagtggtg gggatccaa tcaattgct 
      cagttttca aacatcttg ttcaacggt cgtttccct 
      gtcaaatat tctgtgtgg tgtggtggt gtcgaatgt 
      gaacgtgaa tattgtatt ctattatca taaacaaaa 
      tgaacgccg gtgaaacta tagtaaacg acttgaata 
      aagagtctc tcgattata tcgaaaagg gactgcagt 
      cctttatcg agttccaag agacgtttc gagatctat 
      gtaaggata ttactaggg ttattaacg agtacaccg 
      ctacaggaa ggttaccgg cttttgatc atctgactg 
      atttcgaag agtataaaa agctgaggt cacgtattt 
      caaatgagg aaggatcag tatcatcta aagaaaacc 
      acgaacctg atgatatcc ccacaacaa agaagaaag 
      ctcgccgtt ctcggcgaa ctgcattta aagaagcta 
      ctcaaagca tcagcggtc tcaacgaaa gacgtcacc 
      gcaccaccc tcaaaaagg agaaaatat caatgatca 
      gtggtgaag aatcggtgc ctgagaaaa tttcagtta 
      agaccgaac ttgaagaaa tgcctgctc caactcgac 
      cagctccta aaaagagaa gaaaaaata ctacaagta 
      aacaaatca aacaagaaa aaatagaag atccattag 
      agaatccgc gtgaatcag acgtcatca ttgaagaag 
      ctgtggttc ctttggtta atcttgtgg actcgttgg 
      aagaggaaa ccaataaaa cagaagtgg agtcttcaa 
      agcgaacaa gaactgcgt ccgatgatg gacaaatgg 
      aaaacaaag aaaaaagac caaaatcca gtgaagatg 
      aaactcagt ttgaagacg ctgtttcaa ctgtgttgt 
      cggaaaatg cagacaaaa ataacgcaa cttttgtca 
      ccgcttcta agcttgagg tccagggtg gtaatttaa 
      attcaacag tggttgtac aagatccga aatatctca 
      agagcctga ttccaaaga ccgtagatg atctgatga 
      ccgacgatg aatcagaag aagaaaaag aaacattaa 
      agaaatcga aggtccaag ggactattc ccaagcgcg 
      ctaaacaaa ttttcagtc cgtttgaac aaagccctg 
      tgaagaaaa aggtccagg cttttgaaa aacttcaag 
      aggaagccg ctgccatgc ctgttcgaa tcacccgaa 
      ctaaaacaa gaaatcaag cgaaagaac aagattccg 
      cagcggaag aaccgaatc agaccgaaa aggtgaaaa 
      gtaacgtca aagagaaag cgaaaatat ttactccag 
      tcgtttcga aatttctac caaaagccg ccagcacaa 
      ccgtaaaaa acaagaacc taaagaact cgagttcca 
      ccgaaaacc tgatgatta aaagtgcaa gcgctttaa 
      aagcatctc acgccgaat atcgagaaa ttgagatgc 
      gccgtcacg aaaaggaac aagaagcac tgaaaaaac 
      gcgaagcga tgctccaag cgcaaagtg aagaaaaaa 
      gacgtaaaa gagaagaaa aacaactca aggcgcaac 
      agcaacgtg aaatgattg aaaaagaaa aacagaaag 
      ctttagaag cacaacggt tgaaagagg aacgccatc 
      gacaacata tcgccgaac aagaagaaa agaaactga 
      aattgaaag aagaactgg aaaagaaga gattgatcg 
      tgaaaaacc gagcgattg aacagaaga aatacgagg 
      aagaacaga agagggaag aggaaagaa aagctgcag 
      aattggctg caaaattgg caaagcagg acgaagata 
      ttacaaaga tgcttcaga agcaaaagg aaagaaagg 
      caccgattt atatgagag agccggcac cgcctttgc 
      cgtcagatg actgccatg attcagaca gtgaagaat 
      ataaaaatc aaaaagtca tactaaaaa actgggaaa 
      aagaagtga atgtaatgc ttatgcaaa agtatgaat 
      cggcgatag gtcatgcaa ttaaaaaca cattcttct 
      cacttcaag cacgaacgc cggatctac aagagattt 
      tggaacata tcgaaccga gaaaattga aaagaacat 
      cgagtgctc tctgggacc agccgccac gattcacta 
      tgattccgg ggttagaga ctcatgatg agacagagg 
      aatttattt aattttatt ttatccatt ggttaacag 
      ttttattgc atatcattt catgttttt taaattaca 
      aattatcac caagaatgc gctttccta acaagtttg 
      tccctgttt aacattcag attgcaaat cctttcctg 
      aaattggtt aatcttgca acaaagtcg aggatatta 
      cgctccgtt ttgcagacc cttagacca gttaaaggt 
      agtatgtat tacaataaa aatcgcaca atcac