| General information | |||
| TC033858
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 13840691..13859345 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC033858 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02875 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Days post injection
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
embryo/egg not developed/not fertilized -
Penetrance (percentage of animals/eggs on slide): less than 30%
embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head partially remaining -
Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
posterior terminus not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%
| Comment: usually at least the antenna is visible |
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
posterior terminus not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
- >TC033858 RA
MSRDAYVGG SRSPRGVRL PTVDRSPSR VYPGGSPVG RKPLRQTRS GNNSPEHTY GYHHSPYYR DEDLGSPML EERGRPVAV GPGHHRSRS ATRPANPMS VRYQSLDRT VVDHDREFL PIRDRRDRS LDRGLYFDD EPYSTRSAR QSPTSHQPF IGELQHQNS DLQRELGNL KKELELTNQ KLGSSMHSI KTFWSPELK KERALRKEE SAKYSLIND QLKLLSSEN QVSAITAQL IFIGSVTNS LPENALPNR NNTNDVLLQ FPKHTKKLN QTGGPQILE LNFPAYTKW TQKTR
- >TC033858 RB
MSRDAYVGG SRSPRGVRL PTVDRSPSR VYPGGSPVG RKPLRQTRS GNNSPEHTY GYHHSPYYR DEDLGSPML EERGRPVAV GPGHHRSRS ATRPANPMS VRYQSLDRT VVDHDREFL PIRDRRDRS LDRGLYFDD EPYSTRSAR QSPTSHQPF IGELQHQNS DLQRELGNL KKELELTNQ KLGSSMHSI KTFWSPELK KERALRKEE SAKYSLIND QLKLLSSEN QKQAMLVRQ LEEELRMRM RGPSIEMQQ QMEALYQEN EHLTREIAI LRDTIKELE LRIETQKQT LQARDESIK KLLEMLQNK GMGKEEERQ MFQQMQAMA QKQRASERS AAQACDRCF PVQELQAAN ETLRVLQSQ LELVPSPSS STVRLLLDT KNARIATLE REVNLLESE VERLGGGTQ PPLPSSTAD IPQALPIKR QVSFLLNDV QIGKDGAVI GRLFTTSLS LGTHVSRMD SACLRSCVQ LDEFRVEIQ RRDQEILAM GAKMKTLEE QHQDYQRHI AVLKESLCA KEEHYNMLQ TDVEELRQR LEEKNRHIE KKTQQHQQE RARAAAEIA ELREHMDIK DRKINVLQR KVENLEDLL KEKDNQVDM ARARLSAMQ AHHCSSEGA LSSLEEAIG DKEKQMNQL REQRDRAEQ EKQEERELH EREIAEYKM KIHALNSEV EKLSARLER AQTDRDRLE SKLESSQSE LGKSKAELD KATIEVGRS GADWEQARQ RLARLELEN ERLRQDNER LRQDADRSQ ITFGRNTFS SSHELDRAQ ERVDKTSSD LRRCQAELR VTQADAERA RAEASALQD KLEKSQGEV YRLKARLEN SHQEQDSLR EELERAQST TARLHADKD KAYAELEKA REELERVQA TLGKAQLQQ DKLQNALDK AQTEVDKLQ ERLDKSAGE TRRIQLEKE KLGYDLENI QSQLDKALG QSARIQKER ETAQLEADR LRDKCEKCQ MALTRLQKE KDAYQDDYE KLKEKVEMQ LNQINKIQR ERSDIEHEL DVIKERWEK GHILQQKLQ MERDEAFTE IDILKEKLE KAIYASQKA IDDRENMHK EFEKVLEKY DRSQSDLYR IQNKLDTVQ AEKDRLELE VEKQQLLAT KTREDQRKV QDELQRTQE LYDRASIQL SRTKELEEK SKEELQRMG MDLEMVRDR YEKCQMELR RLQSEKEKF QSENERLQY ELERVHAQS GKAQAAYEK SQEEIARLQ VEVEKAHDK HDKLQNEFR KIVAEYDAL RETNTGPTS RYSKYDRDD RTKEENDRL RAEVERLRE RLDKTLTDL DRSRKDLAL AESTRTKYS YEQEKERSV ELDRLRDEL QRTMGSNQQ LETKLHEVT MQLDLARQE VAKVSGGQD KQRHELERA MIECEKLRD RHDKLKMQI EKYEKENEK LRLELAQAE RRQTLAADK VRNDERLEI ERLKEKLEK AIQARDATE LEAGRLAQE LEKSQMHLA KALETNEAT KIEFERMAN ELARMHERI ERDKLDWKT MEQERDVLR AELQQVRSG LDTQRRTMD HRTQETIVK LQQELAQAS REKEKMASL LEKQGRQGD SIEKQIIKY EADIKQLTM ERDQLVIQL EKSQDMLMN FQQELNQSE AELEKHKAE VARLKAEQK KMSQDVERG TKEIIENRD REITKLRQE LAAVQKERD NHRQRAEKA EKRLQESGA RGDSELEQW RKVVEQETN RADQAEKTA QDLQKRIQV MEKQLQQQL QQMAQYQKE RGIQPPPQD DKELNRLRK ELEKAQMEI KNSSTEKER LQSQLEMLV QELERNQLE LHETTKKMQ SMGAQRGAE DVSAQRRQL EEERKRFEE HRKQVEEQR KAVESKQRQ IEEKERAFA EVDKQLKKR KEQMDQLEI SLQKAGGSA AAAGELNKK LSEAEKNLE KAQEEAKRS AAEMERLLQ LVQMSQEEQ NAKEKQIMD LQQALKTAQ AKLKSQQQV NAQLEEQRK KEEVNKEGD LVIFNLQEK DSELREELL SKQKYIVEL EDTLDSYEQ AMKEYKNEI KILETKLEN KEVTINDLQ NSCIKADDA RRLQKEVDQ KSNIILQLK GQLEGLEQS QLTTDGDFE DLARSSEEK DVRISELEE ALRESMIIS TKREAVLHQ EESKRKQIL EKVSKLEQR LLSLQSAQA MRCHACRPR EALESAISE KDAHLALLE MTGIRNARH ADEADRLRT DKRRLVDRL KKETELSVS LNETDEDER SFYSLYEED EAQKDSLDP T
- >TC033858 RC
MLVRQLEEE LRMRMRGPS IEMQQQMEA LYQENEHLT REIAILRDT IKELELRIE TQKQTLQAR DESIKKLLE MLQNKGMGK EEERQMFQQ MQAMAQKQR ASERSAAQA CDRCFPVQE LQAANETLR VLQSQLELV PSPSSSTVR LLLDTKNAR IATLEREVN LLESEVERL GGGTQPPLP SSTADIPQA LPIKRQVSF LLNDVQIGK DGAVIGRLF TTSLSLGTH VSRMDSACL RSCVQLDEF RVEIQRRDQ EILAMGAKM KTLEEQHQD YQRHIAVLK ESLCAKEEH YNMLQTDVE ELRQRLEEK NRHIEKKTQ QHQQERARA AAEIAELRE HMDIKDRKI NVLQRKVEN LEDLLKEKD NQVDMARAR LSAMQAHHC SSEGALSSL EEAIGDKEK QMNQLREQR DRAEQEKQE ERELHEREI AEYKMKIHA LNSEVEKLS ARLERAQTD RDRLESKLE SSQSELGKS KAELDKATI EVGRSGADW EQARQRLAR LELENERLR QDNERLRQD ADRSQITFG RNTFSSSHE LDRAQERVD KTSSDLRRC QAELRVTQA DAERARAEA SALQDKLEK SQGEVYRLK ARLENSHQE QDSLREELE RAQSTTARL HADKDKAYA ELEKAREEL ERVQATLGK AQLQQDKLQ NALDKAQTE VDKLQERLD KSAGETRRI QLEKEKLGY DLENIQSQL DKALGQSAR IQKERETAQ LEADRLRDK CEKCQMALT RLQKEKDAY QDDYEKLKE KVEMQLNQI NKIQRERSD IEHELDVIK ERWEKGHIL QQKLQMERD EAFTEIDIL KEKLEKAIY ASQKAIDDR ENMHKEFEK VLEKYDRSQ SDLYRIQNK LDTVQAEKD RLELEVEKQ QLLATKTRE DQRKVQDEL QRTQELYDR ASIQLSRTK ELEEKSKEE LQRMGMDLE MVRDRYEKC QMELRRLQS EKEKFQSEN ERLQYELER VHAQSGKAQ AAYEKSQEE IARLQVEVE KAHDKHDKL QNEFRKIVA EYDALRETN TGPTSRYSK YDRDDRTKE ENDRLRAEV ERLRERLDK TLTDLDRSR KDLALAEST RTKYSYEQE KERSVELDR LRDELQRTM GSNQQLETK LHEVTMQLD LARQEVAKV SGGQDKQRH ELERAMIEC EKLRDRHDK LKMQIEKYE KENEKLRLE LAQAERRQT LAADKVRND ERLEIERLK EKLEKAIQA RDATELEAG RLAQELEKS QMHLAKALE TNEATKIEF ERMANELAR MHERIERDK LDWKTMEQE RDVLRAELQ QVRSGLDTQ RRTMDHRTQ ETIVKLQQE LAQASREKE KMASLLEKQ GRQGDSIEK QIIKYEADI KQLTMERDQ LVIQLEKSQ DMLMNFQQE LNQSEAELE KHKAEVARL KAEQKKMSQ DVERGTKEI IENRDREIT KLRQELAAV QKERDNHRQ RAEKAEKRL QESGARGDS ELEQWRKVV EQETNRADQ AEKTAQDLQ KRIQVMEKQ LQQQLQQMA QYQKERGIQ PPPQDDKEL NRLRKELEK AQMEIKNSS TEKERLQSQ LEMLVQELE RNQLELHET TKKMQSMGA QRGAEDVSA QRRQLEEER KRFEEHRKQ VEEQRKAVE SKQRQIEEK ERAFAEVDK QLKKRKEQM DQLEISLQK AGGSAAAAG ELNKKLSEA EKNLEKAQE EAKRSAAEM ERLLQLVQM SQEEQNAKE KQIMDLQQA LKTAQAKLK SQQQVNAQL EEQRKKEEV NKEGDLVIF NLQEKDSEL REELLSKQK YIVELEDTL DSYEQAMKE YKNEIKILE TKLENKEVT INDLQNSCI KADDARRLQ KEVDQKSNI ILQLKGQLE GLEQSQLTT DGDFEDLAR SSEEKDVRI SELEEALRE SMIISTKRE AVLHQEESK RKQILEKVS KLEQRLLSL QSAQAMRCH ACRPREALE SAISEKDAH LALLEMTGI RNARHADEA DRLRTDKRR LVDRLKKET ELSVSLNET DEDERSFYS LYEEDEAQK DSLDPT
- >TC033858 RA
atgtcccga gacgcgtac gtggggggc agccggtcc ccgcggggc gtgagactg cccaccgtg gaccggtcc cccagccga gtgtaccct ggcggcagc cccgtcggc cggaagccc ctgcggcag acccgctcc ggcaacaac tcccccgag cacacctac ggctaccac cactcgccg tactaccgc gacgaggac ctgggcagc cccatgctg gaggagcgc gggcggccc gtagccgtg gggcccggc caccaccgg tcgcggagc gccacgcgg cccgccaac cccatgtcg gtgcgctac cagtcgctg gaccgcacc gtcgtcgac cacgaccgc gagttcctg cccatccgc gaccgccgc gaccgctcc ctcgaccgc ggactctac ttcgacgac gagccctac agcacccgg tccgcgcgg cagtcgccc acctcgcac cagcccttc atcggcgag ctccagcac cagaactcg gacctccag cgcgagctc ggcaacctc aagaaggaa ctcgaactc accaaccaa aagctcggc tcctccatg cactccatc aagaccttc tggagcccg gagctcaag aaggagcgg gcgctcagg aaagaggaa tccgccaag tacagcctc atcaacgat cagctcaaa ttactcagc agtgagaat caggtcagt gccatcact gcgcagctg atatttatt ggctcagtc acaaactca ctcccagag aacgcttta cccaacagg aataacacc aacgatgta ttacttcaa tttcccaaa cacactaag aagcttaat caaactggc ggccctcaa atcctcgag cttaatttc ccagcttat actaaatgg acacagaag acgagataa
- >TC033858 RB
atgtcccga gacgcgtac gtggggggc agccggtcc ccgcggggc gtgagactg cccaccgtg gaccggtcc cccagccga gtgtaccct ggcggcagc cccgtcggc cggaagccc ctgcggcag acccgctcc ggcaacaac tcccccgag cacacctac ggctaccac cactcgccg tactaccgc gacgaggac ctgggcagc cccatgctg gaggagcgc gggcggccc gtagccgtg gggcccggc caccaccgg tcgcggagc gccacgcgg cccgccaac cccatgtcg gtgcgctac cagtcgctg gaccgcacc gtcgtcgac cacgaccgc gagttcctg cccatccgc gaccgccgc gaccgctcc ctcgaccgc ggactctac ttcgacgac gagccctac agcacccgg tccgcgcgg cagtcgccc acctcgcac cagcccttc atcggcgag ctccagcac cagaactcg gacctccag cgcgagctc ggcaacctc aagaaggaa ctcgaactc accaaccaa aagctcggc tcctccatg cactccatc aagaccttc tggagcccg gagctcaag aaggagcgg gcgctcagg aaagaggaa tccgccaag tacagcctc atcaacgat cagctcaaa ttactcagc agtgagaat cagaaacaa gcaatgctg gttcggcag ctggaagag gagttgagg atgcggatg cgtggtcca agcatagag atgcagcag cagatggaa gcgttgtac caagagaat gagcattta actagagag atagccatc ctgcgcgat accataaag gagttggag cttcggatt gagacacaa aagcagact ctgcaagct cgcgatgag agcatcaag aagcttctg gagatgctc cagaacaag ggaatgggt aaagaggag gagaggcag atgttccag caaatgcag gccatggcc caaaagcag cgagcgagc gagcggtcg gcagcacag gcttgtgac cgctgcttc cctgtgcag gagcttcaa gcggccaac gagacgttg cgcgtgctg caatcgcag ctggagctg gtgccctcg ccctccagc agcacggtc agactgctc ctggatacg aaaaacgct agaatagcg actttggag cgcgaggtc aacttgctg gaaagcgag gtggagcgg ctgggcggg ggcacccaa cctcccctt ccgtcgagt accgcggac attcctcag gctcttcct attaagcga caagtaagt tttctgtta aacgatgtg cagatcggg aaggatggc gccgttatt ggtagactt tttaccacc tcactgtct ctcggcacg catgtgtcc cgcatggat agtgcatgc ctccgctca tgtgtgcag ctggacgag ttccgggtg gagatccaa cgacgtgac caggagatc ctcgccatg ggagccaaa atgaaaaca cttgaggaa caacaccaa gactaccag cgccatatc gctgtcctc aaggagtcg ctatgcgct aaagaggaa cactacaac atgctccaa accgatgtc gaggaactc cggcaacga ctcgaagaa aaaaacaga cacatcgaa aagaaaacc caacagcac caacaggaa cgcgctcgg gccgccgcc gaaattgct gaactaaga gaacacatg gacatcaaa gaccgcaaa atcaacgtc cttcaaaga aaggtcgaa aacctcgaa gacctcctt aaagaaaaa gacaaccaa gtcgatatg gccagagcc aggctttcg gcaatgcaa gcccatcat tgctcttcc gaaggtgca ttatcatct ctcgaagag gcaatcggt gacaaggaa aagcaaatg aatcaatta cgcgaacag cgagatcgc gccgaacaa gaaaagcaa gaagagcgc gaactccac gaacgcgaa atagccgaa tacaaaatg aaaatccac gctctaaac agcgaagta gagaaactc agcgcccgc ctcgagcgg gcacaaacc gaccgcgac cgcctcgaa tccaaactg gaaagctct cagtcggaa ttgggcaaa tcaaaggcc gaactcgac aaagcaacg atagaagta ggtcgcagc ggcgccgac tgggagcaa gcccgccag cgtttggcg cgtttagag ctggaaaac gaacggctg agacaggat aacgagagg ctcagacag gatgcggac cggtcgcaa atcacattt ggcagaaat acgttcagc agctcccac gagctggac cgagcgcag gaacgcgtc gataagacg tcctcggac ttgaggaga tgccaggcc gagttaaga gtgacgcaa gccgacgct gagagagcc cgagctgaa gcttcagcc ctgcaggac aagctggaa aagtcgcag ggggaagtg taccgcctc aaggcgcgc ctagagaat tcccatcag gaacaagac agccttcgc gaggagctc gagagagct caatcgact accgccaga ttgcacgca gacaaggac aaagcgtat gcagaattg gagaaggct cgagaggaa ttggagcgt gtacaggcg accttggga aaggctcaa ttacaacaa gacaaatta cagaacgct ctagacaaa gcacagaca gaggtcgac aagttgcaa gaaaggtta gacaagtca gctggggag accagaaga atacaactg gagaaggaa aagctagga tacgattta gagaatatc cagtcgcag ttggacaag gctctaggc cagtctgct cgcattcag aaagaaagg gaaaccgcc caactagaa gcagatcgt cttagagat aagtgtgaa aaatgtcag atggcttta acacgtctc cagaaagag aaagacgcc tatcaagat gattacgaa aaactcaag gaaaaggtt gaaatgcaa ttaaatcaa atcaacaaa attcaaaga gaacgatcc gacatagaa cacgaatta gacgtaatc aaagaaaga tgggagaaa ggacatatc ctccaacaa aaattacaa atggaaaga gacgaggca tttacagaa atcgatatt ttgaaagag aaactagaa aaagccata tatgcctca caaaaggca atagacgat cgagaaaat atgcataaa gaatttgaa aaggtcttg gagaaatac gataggtcc caaagtgat ctctacagg attcaaaat aaattagat acagtgcaa gctgaaaaa gaccgatta gaattagaa gtcgaaaaa caacaatta ctggcaacg aaaacacgc gaggaccaa cgtaaagta caagacgaa ttacagaga actcaggaa ttgtacgat cgagcgtcg attcagtta agccgaacg aaagaattg gaagaaaag tctaaagaa gagctgcag cgtatgggc atggatttg gaaatggtc cgcgacaga tacgaaaaa tgtcaaatg gagcttcgg agattacaa agcgaaaaa gaaaagttc caatcagag aacgaaaga cttcaatac gagctcgaa cgtgtgcac gcacaaagc ggcaaagca caggccgct tacgaaaag agtcaggaa gaaatcgca agattgcaa gtcgaagtt gaaaaagca cacgacaag catgacaaa ctgcaaaac gaattccgt aaaattgtt gccgagtat gacgcctta cgggagacc aacactggt cccacgagt agatactcg aaatatgac cgagatgac agaacgaag gaggaaaat gatagatta cgggccgag gtcgagagg ctgagagag agattagac aagactcta actgatttg gacagatct aggaaagac ttagctttg gccgaaagc acgcgaact aaatacagt tacgagcag gagaaggaa cgctctgtc gaattggat cgtctcaga gacgaatta cagaggact atgggcagt aaccaacag ctcgaaact aaattacac gaagttacg atgcaactg gacctggcg aggcaagaa gtggcgaag gtgtcaggt ggacaagac aaacagcga catgagctg gagcgtgcg atgatcgaa tgcgagaaa ctccgagac agacatgac aagctcaag atgcaaatt gaaaaatat gagaaagag aatgagaaa ttacggctg gagttggct caagccgag cggcggcag acgcttgct gctgacaaa gtgaggaac gacgagcga ttagaaatt gaacgtctt aaagagaaa ctcgagaaa gctatccag gctagagac gccactgaa ctggaagcc ggcagattg gcacaagaa ttggaaaag tcgcagatg catctggcc aaggctttg gaaactaac gaagcaacg aagattgaa ttcgaacgt atggcgaac gaattagcc cgcatgcat gaacgaatc gaaagagac aagctggat tggaagaca atggagcaa gaacgtgac gtgttacga gccgaactg caacaagtg cgaagcgga cttgacact caaagacgc accatggat caccgaaca caagaaaca atcgtcaaa ctccaacaa gagctggct caagcgtcc cgagagaag gaaaaaatg gcctcactc ctagaaaaa caaggccga caaggcgac tccatcgag aaacaaata atcaaatac gaagcggac attaaacaa ttaaccatg gaacgagac caattagta atacaatta gaaaaatcg caagacatg ttaatgaac ttccagcaa gaactcaac cagagtgaa gcggaactg gagaagcac aaagcagaa gtggcccga cttaaagcc gaacagaag aagatgtcg caagacgtg gagcgtgga accaaagaa atcatcgaa aatcgcgac cgggagatc acaaaactg cgccaagaa ctggcggct gtccagaaa gaacgcgat aaccacaga caacgtgca gagaaagct gagaaacga ttacaagag tctggagct cgtggcgat tctgaattg gaacagtgg cgcaaagtt gtcgaacag gaaaccaac agagccgac caagcggag aaaactgcc caagatttg caaaaacgt attcaagtt atggaaaaa caactgcaa cagcagttg caacaaatg gcgcagtac cagaaagaa cgaggtatc caaccacct ccccaggat gataaggaa cttaacagg ctgcgcaaa gaactggag aaggcccaa atggaaatc aaaaatagc agcacggaa aaagaacgg ttacaatcg caacttgaa atgcttgtg caagaattg gaacgaaat cagctcgaa cttcacgaa actacgaag aaaatgcaa agcatgggt gcgcagaga ggcgctgaa gacgtgtca gcacaaagg agacaactg gaggaagaa agaaaacgg tttgaggaa cacagaaaa caagtcgag gagcaaagg aaagccgtg gaaagtaag cagagacag atagaagaa aaggaaaga gcgtttgcc gaggttgac aaacaatta aagaagagg aaagaacaa atggaccaa ttagaaatt tcgttacaa aaagccggc ggaagtgca gctgcagct ggagaacta aacaagaaa ttgtcggaa gccgaaaag aatttagaa aaagctcaa gaagaagca aaacgatca gctgcagaa atggagaga ctattacaa ttagtacaa atgagtcaa gaagagcaa aatgcaaaa gaaaagcag attatggat ttacaacag gcattaaag actgcacag gccaaacta aagagccag caacaagtg aatgcacaa ttagaagaa caaagaaaa aaggaggag gtaaataaa gagggggat cttgtaatt ttcaattta caagaaaaa gatagcgag ttaagagaa gaactttta tcgaaacaa aaatacatt gttgaattg gaggatacc ctggattca tacgagcaa gcaatgaaa gagtacaaa aacgaaatt aaaatactc gaaaccaag ttggagaac aaagaagtg acaattaac gacctgcag aacagctgt atcaaagca gacgatgcg agaagactc cagaaggaa gttgaccag aaatcgaat atcatccta caattgaag ggacaattg gaaggatta gaacaaagt caacttacg actgacgga gattttgaa gatttagct agatcatca gaagaaaaa gatgtcaga attagtgag ttggaagag gcgcttaga gaaagtatg atcatttct actaagcgg gaagcggtt ttgcatcaa gaggagtcg aaaaggaaa caaattttg gaaaaagta tcaaaatta gaacaaaga ctgctgtct ttgcaatct gctcaagca atgcgttgt catgcttgt cgtccacgg gaagctttg gaatctgca atcagtgaa aaggatgca catttagcc ttgctggaa atgacgggg attcgaaat gcaagacac gctgacgaa gccgatcgt cttcgaacg gacaagcga agactggtc gaccgactt aagaaagag actgagttg agcgtttcc ttgaacgaa acggacgaa gatgagcga tccttttac agtttatac gaagaagac gaagctcaa aaagattcc ctcgaccca acttga
- >TC033858 RC
atgctggtt cggcagctg gaagaggag ttgaggatg cggatgcgt ggtccaagc atagagatg cagcagcag atggaagcg ttgtaccaa gagaatgag catttaact agagagata gccatcctg cgcgatacc ataaaggag ttggagctt cggattgag acacaaaag cagactctg caagctcgc gatgagagc atcaagaag cttctggag atgctccag aacaaggga atgggtaaa gaggaggag aggcagatg ttccagcaa atgcaggcc atggcccaa aagcagcga gcgagcgag cggtcggca gcacaggct tgtgaccgc tgcttccct gtgcaggag cttcaagcg gccaacgag acgttgcgc gtgctgcaa tcgcagctg gagctggtg ccctcgccc tccagcagc acggtcaga ctgctcctg gatacgaaa aacgctaga atagcgact ttggagcgc gaggtcaac ttgctggaa agcgaggtg gagcggctg ggcgggggc acccaacct ccccttccg tcgagtacc gcggacatt cctcaggct cttcctatt aagcgacaa gtaagtttt ctgttaaac gatgtgcag atcgggaag gatggcgcc gttattggt agacttttt accacctca ctgtctctc ggcacgcat gtgtcccgc atggatagt gcatgcctc cgctcatgt gtgcagctg gacgagttc cgggtggag atccaacga cgtgaccag gagatcctc gccatggga gccaaaatg aaaacactt gaggaacaa caccaagac taccagcgc catatcgct gtcctcaag gagtcgcta tgcgctaaa gaggaacac tacaacatg ctccaaacc gatgtcgag gaactccgg caacgactc gaagaaaaa aacagacac atcgaaaag aaaacccaa cagcaccaa caggaacgc gctcgggcc gccgccgaa attgctgaa ctaagagaa cacatggac atcaaagac cgcaaaatc aacgtcctt caaagaaag gtcgaaaac ctcgaagac ctccttaaa gaaaaagac aaccaagtc gatatggcc agagccagg ctttcggca atgcaagcc catcattgc tcttccgaa ggtgcatta tcatctctc gaagaggca atcggtgac aaggaaaag caaatgaat caattacgc gaacagcga gatcgcgcc gaacaagaa aagcaagaa gagcgcgaa ctccacgaa cgcgaaata gccgaatac aaaatgaaa atccacgct ctaaacagc gaagtagag aaactcagc gcccgcctc gagcgggca caaaccgac cgcgaccgc ctcgaatcc aaactggaa agctctcag tcggaattg ggcaaatca aaggccgaa ctcgacaaa gcaacgata gaagtaggt cgcagcggc gccgactgg gagcaagcc cgccagcgt ttggcgcgt ttagagctg gaaaacgaa cggctgaga caggataac gagaggctc agacaggat gcggaccgg tcgcaaatc acatttggc agaaatacg ttcagcagc tcccacgag ctggaccga gcgcaggaa cgcgtcgat aagacgtcc tcggacttg aggagatgc caggccgag ttaagagtg acgcaagcc gacgctgag agagcccga gctgaagct tcagccctg caggacaag ctggaaaag tcgcagggg gaagtgtac cgcctcaag gcgcgccta gagaattcc catcaggaa caagacagc cttcgcgag gagctcgag agagctcaa tcgactacc gccagattg cacgcagac aaggacaaa gcgtatgca gaattggag aaggctcga gaggaattg gagcgtgta caggcgacc ttgggaaag gctcaatta caacaagac aaattacag aacgctcta gacaaagca cagacagag gtcgacaag ttgcaagaa aggttagac aagtcagct ggggagacc agaagaata caactggag aaggaaaag ctaggatac gatttagag aatatccag tcgcagttg gacaaggct ctaggccag tctgctcgc attcagaaa gaaagggaa accgcccaa ctagaagca gatcgtctt agagataag tgtgaaaaa tgtcagatg gctttaaca cgtctccag aaagagaaa gacgcctat caagatgat tacgaaaaa ctcaaggaa aaggttgaa atgcaatta aatcaaatc aacaaaatt caaagagaa cgatccgac atagaacac gaattagac gtaatcaaa gaaagatgg gagaaagga catatcctc caacaaaaa ttacaaatg gaaagagac gaggcattt acagaaatc gatattttg aaagagaaa ctagaaaaa gccatatat gcctcacaa aaggcaata gacgatcga gaaaatatg cataaagaa tttgaaaag gtcttggag aaatacgat aggtcccaa agtgatctc tacaggatt caaaataaa ttagataca gtgcaagct gaaaaagac cgattagaa ttagaagtc gaaaaacaa caattactg gcaacgaaa acacgcgag gaccaacgt aaagtacaa gacgaatta cagagaact caggaattg tacgatcga gcgtcgatt cagttaagc cgaacgaaa gaattggaa gaaaagtct aaagaagag ctgcagcgt atgggcatg gatttggaa atggtccgc gacagatac gaaaaatgt caaatggag cttcggaga ttacaaagc gaaaaagaa aagttccaa tcagagaac gaaagactt caatacgag ctcgaacgt gtgcacgca caaagcggc aaagcacag gccgcttac gaaaagagt caggaagaa atcgcaaga ttgcaagtc gaagttgaa aaagcacac gacaagcat gacaaactg caaaacgaa ttccgtaaa attgttgcc gagtatgac gccttacgg gagaccaac actggtccc acgagtaga tactcgaaa tatgaccga gatgacaga acgaaggag gaaaatgat agattacgg gccgaggtc gagaggctg agagagaga ttagacaag actctaact gatttggac agatctagg aaagactta gctttggcc gaaagcacg cgaactaaa tacagttac gagcaggag aaggaacgc tctgtcgaa ttggatcgt ctcagagac gaattacag aggactatg ggcagtaac caacagctc gaaactaaa ttacacgaa gttacgatg caactggac ctggcgagg caagaagtg gcgaaggtg tcaggtgga caagacaaa cagcgacat gagctggag cgtgcgatg atcgaatgc gagaaactc cgagacaga catgacaag ctcaagatg caaattgaa aaatatgag aaagagaat gagaaatta cggctggag ttggctcaa gccgagcgg cggcagacg cttgctgct gacaaagtg aggaacgac gagcgatta gaaattgaa cgtcttaaa gagaaactc gagaaagct atccaggct agagacgcc actgaactg gaagccggc agattggca caagaattg gaaaagtcg cagatgcat ctggccaag gctttggaa actaacgaa gcaacgaag attgaattc gaacgtatg gcgaacgaa ttagcccgc atgcatgaa cgaatcgaa agagacaag ctggattgg aagacaatg gagcaagaa cgtgacgtg ttacgagcc gaactgcaa caagtgcga agcggactt gacactcaa agacgcacc atggatcac cgaacacaa gaaacaatc gtcaaactc caacaagag ctggctcaa gcgtcccga gagaaggaa aaaatggcc tcactccta gaaaaacaa ggccgacaa ggcgactcc atcgagaaa caaataatc aaatacgaa gcggacatt aaacaatta accatggaa cgagaccaa ttagtaata caattagaa aaatcgcaa gacatgtta atgaacttc cagcaagaa ctcaaccag agtgaagcg gaactggag aagcacaaa gcagaagtg gcccgactt aaagccgaa cagaagaag atgtcgcaa gacgtggag cgtggaacc aaagaaatc atcgaaaat cgcgaccgg gagatcaca aaactgcgc caagaactg gcggctgtc cagaaagaa cgcgataac cacagacaa cgtgcagag aaagctgag aaacgatta caagagtct ggagctcgt ggcgattct gaattggaa cagtggcgc aaagttgtc gaacaggaa accaacaga gccgaccaa gcggagaaa actgcccaa gatttgcaa aaacgtatt caagttatg gaaaaacaa ctgcaacag cagttgcaa caaatggcg cagtaccag aaagaacga ggtatccaa ccacctccc caggatgat aaggaactt aacaggctg cgcaaagaa ctggagaag gcccaaatg gaaatcaaa aatagcagc acggaaaaa gaacggtta caatcgcaa cttgaaatg cttgtgcaa gaattggaa cgaaatcag ctcgaactt cacgaaact acgaagaaa atgcaaagc atgggtgcg cagagaggc gctgaagac gtgtcagca caaaggaga caactggag gaagaaaga aaacggttt gaggaacac agaaaacaa gtcgaggag caaaggaaa gccgtggaa agtaagcag agacagata gaagaaaag gaaagagcg tttgccgag gttgacaaa caattaaag aagaggaaa gaacaaatg gaccaatta gaaatttcg ttacaaaaa gccggcgga agtgcagct gcagctgga gaactaaac aagaaattg tcggaagcc gaaaagaat ttagaaaaa gctcaagaa gaagcaaaa cgatcagct gcagaaatg gagagacta ttacaatta gtacaaatg agtcaagaa gagcaaaat gcaaaagaa aagcagatt atggattta caacaggca ttaaagact gcacaggcc aaactaaag agccagcaa caagtgaat gcacaatta gaagaacaa agaaaaaag gaggaggta aataaagag ggggatctt gtaattttc aatttacaa gaaaaagat agcgagtta agagaagaa cttttatcg aaacaaaaa tacattgtt gaattggag gataccctg gattcatac gagcaagca atgaaagag tacaaaaac gaaattaaa atactcgaa accaagttg gagaacaaa gaagtgaca attaacgac ctgcagaac agctgtatc aaagcagac gatgcgaga agactccag aaggaagtt gaccagaaa tcgaatatc atcctacaa ttgaaggga caattggaa ggattagaa caaagtcaa cttacgact gacggagat tttgaagat ttagctaga tcatcagaa gaaaaagat gtcagaatt agtgagttg gaagaggcg cttagagaa agtatgatc atttctact aagcgggaa gcggttttg catcaagag gagtcgaaa aggaaacaa attttggaa aaagtatca aaattagaa caaagactg ctgtctttg caatctgct caagcaatg cgttgtcat gcttgtcgt ccacgggaa gctttggaa tctgcaatc agtgaaaag gatgcacat ttagccttg ctggaaatg acggggatt cgaaatgca agacacgct gacgaagcc gatcgtctt cgaacggac aagcgaaga ctggtcgac cgacttaag aaagagact gagttgagc gtttccttg aacgaaacg gacgaagat gagcgatcc ttttacagt ttatacgaa gaagacgaa gctcaaaaa gattccctc gacccaact tga
- >TC033858 RA
gatcagaat tcggaacgg aaagtagag tgcttgagc ggagcgcgg acgacatct tcagtggcc gtgagtgtc atgtcatga gatgtcccg agacgcgta cgtgggggg cagccggtc cccgcgggg cgtgagact gcccaccgt ggaccggtc ccccagccg agtgtaccc tggcggcag ccccgtcgg ccggaagcc cctgcggca gacccgctc cggcaacaa ctcccccga gcacaccta cggctacca ccactcgcc gtactaccg cgacgagga cctgggcag ccccatgct ggaggagcg cgggcggcc cgtagccgt ggggcccgg ccaccaccg gtcgcggag cgccacgcg gcccgccaa ccccatgtc ggtgcgcta ccagtcgct ggaccgcac cgtcgtcga ccacgaccg cgagttcct gcccatccg cgaccgccg cgaccgctc cctcgaccg cggactcta cttcgacga cgagcccta cagcacccg gtccgcgcg gcagtcgcc cacctcgca ccagccctt catcggcga gctccagca ccagaactc ggacctcca gcgcgagct cggcaacct caagaagga actcgaact caccaacca aaagctcgg ctcctccat gcactccat caagacctt ctggagccc ggagctcaa gaaggagcg ggcgctcag gaaagagga atccgccaa gtacagcct catcaacga tcagctcaa attactcag cagtgagaa tcaggtcag tgccatcac tgcgcagct gatatttat tggctcagt cacaaactc actcccaga gaacgcttt acccaacag gaataacac caacgatgt attacttca atttcccaa acacactaa gaagcttaa tcaaactgg cggccctca aatcctcga gcttaattt cccagctta tactaaatg gacacagaa gacgagata aatcaaatg cgaggccac accagaaaa taacgagag caaaggcat agagcaaga accaaaaat ttctaaaaa aactgtgcc ccttgtcct ttcacctta tccaatttt cacgaggag taaaccgca attccaatc gctacacca gttttcctt ttttgcttc gttttctct atgattttc cctgttttt tttttcaag attatttca tgttctttt ttaataatg taatataaa tattagaac aaa
- >TC033858 RB
gatcagaat tcggaacgg aaagtagag tgcttgagc ggagcgcgg acgacatct tcagtggcc gtgagtgtc atgtcatga gatgtcccg agacgcgta cgtgggggg cagccggtc cccgcgggg cgtgagact gcccaccgt ggaccggtc ccccagccg agtgtaccc tggcggcag ccccgtcgg ccggaagcc cctgcggca gacccgctc cggcaacaa ctcccccga gcacaccta cggctacca ccactcgcc gtactaccg cgacgagga cctgggcag ccccatgct ggaggagcg cgggcggcc cgtagccgt ggggcccgg ccaccaccg gtcgcggag cgccacgcg gcccgccaa ccccatgtc ggtgcgcta ccagtcgct ggaccgcac cgtcgtcga ccacgaccg cgagttcct gcccatccg cgaccgccg cgaccgctc cctcgaccg cggactcta cttcgacga cgagcccta cagcacccg gtccgcgcg gcagtcgcc cacctcgca ccagccctt catcggcga gctccagca ccagaactc ggacctcca gcgcgagct cggcaacct caagaagga actcgaact caccaacca aaagctcgg ctcctccat gcactccat caagacctt ctggagccc ggagctcaa gaaggagcg ggcgctcag gaaagagga atccgccaa gtacagcct catcaacga tcagctcaa attactcag cagtgagaa tcagaaaca agcaatgct ggttcggca gctggaaga ggagttgag gatgcggat gcgtggtcc aagcataga gatgcagca gcagatgga agcgttgta ccaagagaa tgagcattt aactagaga gatagccat cctgcgcga taccataaa ggagttgga gcttcggat tgagacaca aaagcagac tctgcaagc tcgcgatga gagcatcaa gaagcttct ggagatgct ccagaacaa gggaatggg taaagagga ggagaggca gatgttcca gcaaatgca ggccatggc ccaaaagca gcgagcgag cgagcggtc ggcagcaca ggcttgtga ccgctgctt ccctgtgca ggagcttca agcggccaa cgagacgtt gcgcgtgct gcaatcgca gctggagct ggtgccctc gccctccag cagcacggt cagactgct cctggatac gaaaaacgc tagaatagc gactttgga gcgcgaggt caacttgct ggaaagcga ggtggagcg gctgggcgg gggcaccca acctcccct tccgtcgag taccgcgga cattcctca ggctcttcc tattaagcg acaagtaag ttttctgtt aaacgatgt gcagatcgg gaaggatgg cgccgttat tggtagact ttttaccac ctcactgtc tctcggcac gcatgtgtc ccgcatgga tagtgcatg cctccgctc atgtgtgca gctggacga gttccgggt ggagatcca acgacgtga ccaggagat cctcgccat gggagccaa aatgaaaac acttgagga acaacacca agactacca gcgccatat cgctgtcct caaggagtc gctatgcgc taaagagga acactacaa catgctcca aaccgatgt cgaggaact ccggcaacg actcgaaga aaaaaacag acacatcga aaagaaaac ccaacagca ccaacagga acgcgctcg ggccgccgc cgaaattgc tgaactaag agaacacat ggacatcaa agaccgcaa aatcaacgt ccttcaaag aaaggtcga aaacctcga agacctcct taaagaaaa agacaacca agtcgatat ggccagagc caggctttc ggcaatgca agcccatca ttgctcttc cgaaggtgc attatcatc tctcgaaga ggcaatcgg tgacaagga aaagcaaat gaatcaatt acgcgaaca gcgagatcg cgccgaaca agaaaagca agaagagcg cgaactcca cgaacgcga aatagccga atacaaaat gaaaatcca cgctctaaa cagcgaagt agagaaact cagcgcccg cctcgagcg ggcacaaac cgaccgcga ccgcctcga atccaaact ggaaagctc tcagtcgga attgggcaa atcaaaggc cgaactcga caaagcaac gatagaagt aggtcgcag cggcgccga ctgggagca agcccgcca gcgtttggc gcgtttaga gctggaaaa cgaacggct gagacagga taacgagag gctcagaca ggatgcgga ccggtcgca aatcacatt tggcagaaa tacgttcag cagctccca cgagctgga ccgagcgca ggaacgcgt cgataagac gtcctcgga cttgaggag atgccaggc cgagttaag agtgacgca agccgacgc tgagagagc ccgagctga agcttcagc cctgcagga caagctgga aaagtcgca gggggaagt gtaccgcct caaggcgcg cctagagaa ttcccatca ggaacaaga cagccttcg cgaggagct cgagagagc tcaatcgac taccgccag attgcacgc agacaagga caaagcgta tgcagaatt ggagaaggc tcgagagga attggagcg tgtacaggc gaccttggg aaaggctca attacaaca agacaaatt acagaacgc tctagacaa agcacagac agaggtcga caagttgca agaaaggtt agacaagtc agctgggga gaccagaag aatacaact ggagaagga aaagctagg atacgattt agagaatat ccagtcgca gttggacaa ggctctagg ccagtctgc tcgcattca gaaagaaag ggaaaccgc ccaactaga agcagatcg tcttagaga taagtgtga aaaatgtca gatggcttt aacacgtct ccagaaaga gaaagacgc ctatcaaga tgattacga aaaactcaa ggaaaaggt tgaaatgca attaaatca aatcaacaa aattcaaag agaacgatc cgacataga acacgaatt agacgtaat caaagaaag atgggagaa aggacatat cctccaaca aaaattaca aatggaaag agacgaggc atttacaga aatcgatat tttgaaaga gaaactaga aaaagccat atatgcctc acaaaaggc aatagacga tcgagaaaa tatgcataa agaatttga aaaggtctt ggagaaata cgataggtc ccaaagtga tctctacag gattcaaaa taaattaga tacagtgca agctgaaaa agaccgatt agaattaga agtcgaaaa acaacaatt actggcaac gaaaacacg cgaggacca acgtaaagt acaagacga attacagag aactcagga attgtacga tcgagcgtc gattcagtt aagccgaac gaaagaatt ggaagaaaa gtctaaaga agagctgca gcgtatggg catggattt ggaaatggt ccgcgacag atacgaaaa atgtcaaat ggagcttcg gagattaca aagcgaaaa agaaaagtt ccaatcaga gaacgaaag acttcaata cgagctcga acgtgtgca cgcacaaag cggcaaagc acaggccgc ttacgaaaa gagtcagga agaaatcgc aagattgca agtcgaagt tgaaaaagc acacgacaa gcatgacaa actgcaaaa cgaattccg taaaattgt tgccgagta tgacgcctt acgggagac caacactgg tcccacgag tagatactc gaaatatga ccgagatga cagaacgaa ggaggaaaa tgatagatt acgggccga ggtcgagag gctgagaga gagattaga caagactct aactgattt ggacagatc taggaaaga cttagcttt ggccgaaag cacgcgaac taaatacag ttacgagca ggagaagga acgctctgt cgaattgga tcgtctcag agacgaatt acagaggac tatgggcag taaccaaca gctcgaaac taaattaca cgaagttac gatgcaact ggacctggc gaggcaaga agtggcgaa ggtgtcagg tggacaaga caaacagcg acatgagct ggagcgtgc gatgatcga atgcgagaa actccgaga cagacatga caagctcaa gatgcaaat tgaaaaata tgagaaaga gaatgagaa attacggct ggagttggc tcaagccga gcggcggca gacgcttgc tgctgacaa agtgaggaa cgacgagcg attagaaat tgaacgtct taaagagaa actcgagaa agctatcca ggctagaga cgccactga actggaagc cggcagatt ggcacaaga attggaaaa gtcgcagat gcatctggc caaggcttt ggaaactaa cgaagcaac gaagattga attcgaacg tatggcgaa cgaattagc ccgcatgca tgaacgaat cgaaagaga caagctgga ttggaagac aatggagca agaacgtga cgtgttacg agccgaact gcaacaagt gcgaagcgg acttgacac tcaaagacg caccatgga tcaccgaac acaagaaac aatcgtcaa actccaaca agagctggc tcaagcgtc ccgagagaa ggaaaaaat ggcctcact cctagaaaa acaaggccg acaaggcga ctccatcga gaaacaaat aatcaaata cgaagcgga cattaaaca attaaccat ggaacgaga ccaattagt aatacaatt agaaaaatc gcaagacat gttaatgaa cttccagca agaactcaa ccagagtga agcggaact ggagaagca caaagcaga agtggcccg acttaaagc cgaacagaa gaagatgtc gcaagacgt ggagcgtgg aaccaaaga aatcatcga aaatcgcga ccgggagat cacaaaact gcgccaaga actggcggc tgtccagaa agaacgcga taaccacag acaacgtgc agagaaagc tgagaaacg attacaaga gtctggagc tcgtggcga ttctgaatt ggaacagtg gcgcaaagt tgtcgaaca ggaaaccaa cagagccga ccaagcgga gaaaactgc ccaagattt gcaaaaacg tattcaagt tatggaaaa acaactgca acagcagtt gcaacaaat ggcgcagta ccagaaaga acgaggtat ccaaccacc tccccagga tgataagga acttaacag gctgcgcaa agaactgga gaaggccca aatggaaat caaaaatag cagcacgga aaaagaacg gttacaatc gcaacttga aatgcttgt gcaagaatt ggaacgaaa tcagctcga acttcacga aactacgaa gaaaatgca aagcatggg tgcgcagag aggcgctga agacgtgtc agcacaaag gagacaact ggaggaaga aagaaaacg gtttgagga acacagaaa acaagtcga ggagcaaag gaaagccgt ggaaagtaa gcagagaca gatagaaga aaaggaaag agcgtttgc cgaggttga caaacaatt aaagaagag gaaagaaca aatggacca attagaaat ttcgttaca aaaagccgg cggaagtgc agctgcagc tggagaact aaacaagaa attgtcgga agccgaaaa gaatttaga aaaagctca agaagaagc aaaacgatc agctgcaga aatggagag actattaca attagtaca aatgagtca agaagagca aaatgcaaa agaaaagca gattatgga tttacaaca ggcattaaa gactgcaca ggccaaact aaagagcca gcaacaagt gaatgcaca attagaaga acaaagaaa aaaggagga ggtaaataa agaggggga tcttgtaat tttcaattt acaagaaaa agatagcga gttaagaga agaactttt atcgaaaca aaaatacat tgttgaatt ggaggatac cctggattc atacgagca agcaatgaa agagtacaa aaacgaaat taaaatact cgaaaccaa gttggagaa caaagaagt gacaattaa cgacctgca gaacagctg tatcaaagc agacgatgc gagaagact ccagaagga agttgacca gaaatcgaa tatcatcct acaattgaa gggacaatt ggaaggatt agaacaaag tcaacttac gactgacgg agattttga agatttagc tagatcatc agaagaaaa agatgtcag aattagtga gttggaaga ggcgcttag agaaagtat gatcatttc tactaagcg ggaagcggt tttgcatca agaggagtc gaaaaggaa acaaatttt ggaaaaagt atcaaaatt agaacaaag actgctgtc tttgcaatc tgctcaagc aatgcgttg tcatgcttg tcgtccacg ggaagcttt ggaatctgc aatcagtga aaaggatgc acatttagc cttgctgga aatgacggg gattcgaaa tgcaagaca cgctgacga agccgatcg tcttcgaac ggacaagcg aagactggt cgaccgact taagaaaga gactgagtt gagcgtttc cttgaacga aacggacga agatgagcg atcctttta cagtttata cgaagaaga cgaagctca aaaagattc cctcgaccc aacttgagc taaaaccaa atttaccaa agattccaa tgatctcag caataaaga atatttata ggcaaccgt tatcaggtt gccgccatt atttcagaa gcactgcct gtaaacttg tattaagta ccacataaa cattgtgaa cgtgttagt ttattattg ttacctgtt cgagtttag acatgacaa taattaatg gaatggggc agaattgtt tccggtagt tcggttgtg ttactgaaa catttaaaa acactcaac attggcgaa ttttttgtg atctgtttt attctgcag tgttggact tgatttcga tgctctctt gtaaagcgg ctataattt ttttataga tcattttta aattacaaa tcgatgcgg ccgacccga gtccggtta ctttaagtg cgctgtgcg tgtatgtgt gaatttttg ccaccgttt gcggccctg ataagtatt aaatatttt atacaaaac tagttcagc gaaaattag aaaaaccca aatttgagt gacattcct gtagcaacg tttcgaaca ttccgttag agtttaggt tgaaaacga cgctgcaag tggtggcat agcgcctcc tattttgta aaaagctgg tatgttata ctttgagta caaatgaaa tattgtatt tctccatgc aaatacgta ttacacatt tgtcatcta aaacgttcg gagtaaata atttcaaat gccttgtat atcaatttg tacattcat tatcatatc ataggccga gcaacactg ccatctcgt gccattatc ataataaac agcgtctga gagcccctg taaatagtg cgcccagcc agcttctcc acgttgtgt ctctccgta cgtagcggt agaatttac aagagtaga tagatgaag accaagtgc gcaaacgca cattttatt aattattat ctctattta ttaattctc ttccgtgtc gcttttaga acatctact ggttgggcg aacatgcgg gtgtcgttt ctacttgtg cccgcattt tagggactg atgagccct ggtccgcaa ctctccact cttcctctg ctgttttgt tgccatcca atgaataac atttcaaat ttgaaccaa acgcctgca aacgtcctt acatcaagt ctaagaggg ttcctacag ggagttgcg gaccagcgc gccctaaac tttgttaag cgaatttaa ctctaatct ataataaaa tttcatccc tcagtgtta aagtatatt atggcaaat attaaacta agcacaata agtatgtaa caacggtta tgatgtata gttagggta gtcacgact tgaaagatt cattatcag aggttttga tgtaaataa ttttgttga tttcttcct aatcgcggt tcaagcttc gtttcattg taacaggat caaacaaca gttttcgtt tggtttgct taatcgcat tgtgtgtct gttatgaat aataacgca tctaatata taatttaat ggcataata tcatctctt cctcttgac acccatcga aacctcacc acttaatgt cacctaaca tatctcatc caaacttat atcaaattg ctttttata taatattaa taaaatttg gaacttgga ataggatgg ataaaattg ttagcagtt acaataata aatgttaat tgttgagta cagacgttg tacgaaata atttcgtct gcagttgag gttttaacg acgtaagca atctattaa ccgggacag ctaccactt cagtcggaa cccttcaag tgccatgtg tgcttccga aattacccc atgagggct cttatgcat ctgttaatg gcttgctta cattttaaa atgtataga aaactagtc taagtagtt catgttact ttcttaagt tggtactga tttatttat gtcacgctg ttgtaaaaa catgttaag aacgatgta tgaaagtgt gaaaagaaa gtgatacaa aaaca
- >TC033858 RC
tctcatgaa tatggcgtg gtgggtaaa ttttattga agcttgatg ttttcgaca ctttatgat ttatgaatt ttctaattt ttctaccta attgcgtat ttatactaa aaacagagg acactttta tagtttgcc tgtcgttca tgaacataa agtcttggc acggaaagc accgacgaa tcgcttcca ccgcagaac tgaaacaaa attaaatat tcagggata aataacaaa gaaaatgtc cccaacaag gaacaaaga caaacaagc aatgctggt tcggcagct ggaagagga gttgaggat gcggatgcg tggtccaag catagagat gcagcagca gatggaagc gttgtacca agagaatga gcatttaac tagagagat agccatcct gcgcgatac cataaagga gttggagct tcggattga gacacaaaa gcagactct gcaagctcg cgatgagag catcaagaa gcttctgga gatgctcca gaacaaggg aatgggtaa agaggagga gaggcagat gttccagca aatgcaggc catggccca aaagcagcg agcgagcga gcggtcggc agcacaggc ttgtgaccg ctgcttccc tgtgcagga gcttcaagc ggccaacga gacgttgcg cgtgctgca atcgcagct ggagctggt gccctcgcc ctccagcag cacggtcag actgctcct ggatacgaa aaacgctag aatagcgac tttggagcg cgaggtcaa cttgctgga aagcgaggt ggagcggct gggcggggg cacccaacc tccccttcc gtcgagtac cgcggacat tcctcaggc tcttcctat taagcgaca agtaagttt tctgttaaa cgatgtgca gatcgggaa ggatggcgc cgttattgg tagactttt taccacctc actgtctct cggcacgca tgtgtcccg catggatag tgcatgcct ccgctcatg tgtgcagct ggacgagtt ccgggtgga gatccaacg acgtgacca ggagatcct cgccatggg agccaaaat gaaaacact tgaggaaca acaccaaga ctaccagcg ccatatcgc tgtcctcaa ggagtcgct atgcgctaa agaggaaca ctacaacat gctccaaac cgatgtcga ggaactccg gcaacgact cgaagaaaa aaacagaca catcgaaaa gaaaaccca acagcacca acaggaacg cgctcgggc cgccgccga aattgctga actaagaga acacatgga catcaaaga ccgcaaaat caacgtcct tcaaagaaa ggtcgaaaa cctcgaaga cctccttaa agaaaaaga caaccaagt cgatatggc cagagccag gctttcggc aatgcaagc ccatcattg ctcttccga aggtgcatt atcatctct cgaagaggc aatcggtga caaggaaaa gcaaatgaa tcaattacg cgaacagcg agatcgcgc cgaacaaga aaagcaaga agagcgcga actccacga acgcgaaat agccgaata caaaatgaa aatccacgc tctaaacag cgaagtaga gaaactcag cgcccgcct cgagcgggc acaaaccga ccgcgaccg cctcgaatc caaactgga aagctctca gtcggaatt gggcaaatc aaaggccga actcgacaa agcaacgat agaagtagg tcgcagcgg cgccgactg ggagcaagc ccgccagcg tttggcgcg tttagagct ggaaaacga acggctgag acaggataa cgagaggct cagacagga tgcggaccg gtcgcaaat cacatttgg cagaaatac gttcagcag ctcccacga gctggaccg agcgcagga acgcgtcga taagacgtc ctcggactt gaggagatg ccaggccga gttaagagt gacgcaagc cgacgctga gagagcccg agctgaagc ttcagccct gcaggacaa gctggaaaa gtcgcaggg ggaagtgta ccgcctcaa ggcgcgcct agagaattc ccatcagga acaagacag ccttcgcga ggagctcga gagagctca atcgactac cgccagatt gcacgcaga caaggacaa agcgtatgc agaattgga gaaggctcg agaggaatt ggagcgtgt acaggcgac cttgggaaa ggctcaatt acaacaaga caaattaca gaacgctct agacaaagc acagacaga ggtcgacaa gttgcaaga aaggttaga caagtcagc tggggagac cagaagaat acaactgga gaaggaaaa gctaggata cgatttaga gaatatcca gtcgcagtt ggacaaggc tctaggcca gtctgctcg cattcagaa agaaaggga aaccgccca actagaagc agatcgtct tagagataa gtgtgaaaa atgtcagat ggctttaac acgtctcca gaaagagaa agacgccta tcaagatga ttacgaaaa actcaagga aaaggttga aatgcaatt aaatcaaat caacaaaat tcaaagaga acgatccga catagaaca cgaattaga cgtaatcaa agaaagatg ggagaaagg acatatcct ccaacaaaa attacaaat ggaaagaga cgaggcatt tacagaaat cgatatttt gaaagagaa actagaaaa agccatata tgcctcaca aaaggcaat agacgatcg agaaaatat gcataaaga atttgaaaa ggtcttgga gaaatacga taggtccca aagtgatct ctacaggat tcaaaataa attagatac agtgcaagc tgaaaaaga ccgattaga attagaagt cgaaaaaca acaattact ggcaacgaa aacacgcga ggaccaacg taaagtaca agacgaatt acagagaac tcaggaatt gtacgatcg agcgtcgat tcagttaag ccgaacgaa agaattgga agaaaagtc taaagaaga gctgcagcg tatgggcat ggatttgga aatggtccg cgacagata cgaaaaatg tcaaatgga gcttcggag attacaaag cgaaaaaga aaagttcca atcagagaa cgaaagact tcaatacga gctcgaacg tgtgcacgc acaaagcgg caaagcaca ggccgctta cgaaaagag tcaggaaga aatcgcaag attgcaagt cgaagttga aaaagcaca cgacaagca tgacaaact gcaaaacga attccgtaa aattgttgc cgagtatga cgccttacg ggagaccaa cactggtcc cacgagtag atactcgaa atatgaccg agatgacag aacgaagga ggaaaatga tagattacg ggccgaggt cgagaggct gagagagag attagacaa gactctaac tgatttgga cagatctag gaaagactt agctttggc cgaaagcac gcgaactaa atacagtta cgagcagga gaaggaacg ctctgtcga attggatcg tctcagaga cgaattaca gaggactat gggcagtaa ccaacagct cgaaactaa attacacga agttacgat gcaactgga cctggcgag gcaagaagt ggcgaaggt gtcaggtgg acaagacaa acagcgaca tgagctgga gcgtgcgat gatcgaatg cgagaaact ccgagacag acatgacaa gctcaagat gcaaattga aaaatatga gaaagagaa tgagaaatt acggctgga gttggctca agccgagcg gcggcagac gcttgctgc tgacaaagt gaggaacga cgagcgatt agaaattga acgtcttaa agagaaact cgagaaagc tatccaggc tagagacgc cactgaact ggaagccgg cagattggc acaagaatt ggaaaagtc gcagatgca tctggccaa ggctttgga aactaacga agcaacgaa gattgaatt cgaacgtat ggcgaacga attagcccg catgcatga acgaatcga aagagacaa gctggattg gaagacaat ggagcaaga acgtgacgt gttacgagc cgaactgca acaagtgcg aagcggact tgacactca aagacgcac catggatca ccgaacaca agaaacaat cgtcaaact ccaacaaga gctggctca agcgtcccg agagaagga aaaaatggc ctcactcct agaaaaaca aggccgaca aggcgactc catcgagaa acaaataat caaatacga agcggacat taaacaatt aaccatgga acgagacca attagtaat acaattaga aaaatcgca agacatgtt aatgaactt ccagcaaga actcaacca gagtgaagc ggaactgga gaagcacaa agcagaagt ggcccgact taaagccga acagaagaa gatgtcgca agacgtgga gcgtggaac caaagaaat catcgaaaa tcgcgaccg ggagatcac aaaactgcg ccaagaact ggcggctgt ccagaaaga acgcgataa ccacagaca acgtgcaga gaaagctga gaaacgatt acaagagtc tggagctcg tggcgattc tgaattgga acagtggcg caaagttgt cgaacagga aaccaacag agccgacca agcggagaa aactgccca agatttgca aaaacgtat tcaagttat ggaaaaaca actgcaaca gcagttgca acaaatggc gcagtacca gaaagaacg aggtatcca accacctcc ccaggatga taaggaact taacaggct gcgcaaaga actggagaa ggcccaaat ggaaatcaa aaatagcag cacggaaaa agaacggtt acaatcgca acttgaaat gcttgtgca agaattgga acgaaatca gctcgaact tcacgaaac tacgaagaa aatgcaaag catgggtgc gcagagagg cgctgaaga cgtgtcagc acaaaggag acaactgga ggaagaaag aaaacggtt tgaggaaca cagaaaaca agtcgagga gcaaaggaa agccgtgga aagtaagca gagacagat agaagaaaa ggaaagagc gtttgccga ggttgacaa acaattaaa gaagaggaa agaacaaat ggaccaatt agaaatttc gttacaaaa agccggcgg aagtgcagc tgcagctgg agaactaaa caagaaatt gtcggaagc cgaaaagaa tttagaaaa agctcaaga agaagcaaa acgatcagc tgcagaaat ggagagact attacaatt agtacaaat gagtcaaga agagcaaaa tgcaaaaga aaagcagat tatggattt acaacaggc attaaagac tgcacaggc caaactaaa gagccagca acaagtgaa tgcacaatt agaagaaca aagaaaaaa ggaggaggt aaataaaga gggggatct tgtaatttt caatttaca agaaaaaga tagcgagtt aagagaaga acttttatc gaaacaaaa atacattgt tgaattgga ggataccct ggattcata cgagcaagc aatgaaaga gtacaaaaa cgaaattaa aatactcga aaccaagtt ggagaacaa agaagtgac aattaacga cctgcagaa cagctgtat caaagcaga cgatgcgag aagactcca gaaggaagt tgaccagaa atcgaatat catcctaca attgaaggg acaattgga aggattaga acaaagtca acttacgac tgacggaga ttttgaaga tttagctag atcatcaga agaaaaaga tgtcagaat tagtgagtt ggaagaggc gcttagaga aagtatgat catttctac taagcggga agcggtttt gcatcaaga ggagtcgaa aaggaaaca aattttgga aaaagtatc aaaattaga acaaagact gctgtcttt gcaatctgc tcaagcaat gcgttgtca tgcttgtcg tccacggga agctttgga atctgcaat cagtgaaaa ggatgcaca tttagcctt gctggaaat gacggggat tcgaaatgc aagacacgc tgacgaagc cgatcgtct tcgaacgga caagcgaag actggtcga ccgacttaa gaaagagac tgagttgag cgtttcctt gaacgaaac ggacgaaga tgagcgatc cttttacag tttatacga agaagacga agctcaaaa agattccct cgacccaac ttgagctaa aaccaaatt taccaaaga ttccaatga tctcagcaa taaagaata tttataggc aaccgttat caggttgcc gccattatt tcagaagca ctgcctgta aacttgtat taagtacca cataaacat tgtgaacgt gttagttta ttattgtta cctgttcga gtttagaca tgacaataa ttaatggaa tggggcaga attgtttcc ggtagttcg gttgtgtta ctgaaacat ttaaaaaca ctcaacatt ggcgaattt tttgtgatc tgttttatt ctgcagtgt tggacttga tttcgatgc tctcttgta aagcggcta taatttttt tatagatca tttttaaat tacaaatcg atgcggccg acccgagtc cggttactt taagtgcgc tgtgcgtgt atgtgtgaa tttttgcca ccgtttgcg gccctgata agtattaaa tattttata caaaactag ttcagcgaa aattagaaa aacccaaat ttgagtgac attcctgta gcaacgttt cgaacattc cgttagagt ttaggttga aaacgacgc tgcaagtgg tggcatagc gcctcctat tttgtaaaa agctggtat gttatactt tgagtacaa atgaaatat tgtatttct ccatgcaaa tacgtatta cacatttgt catctaaaa cgttcggag taaataatt tcaaatgcc ttgtatatc aatttgtac attcattat catatcata ggccgagca acactgcca tctcgtgcc attatcata ataaacagc gtctgagag cccctgtaa atagtgcgc ccagccagc ttctccacg ttgtgtctc tccgtacgt agcggtaga atttacaag agtagatag atgaagacc aagtgcgca aacgcacat tttattaat tattatctc tatttatta attctcttc cgtgtcgct tttagaaca tctactggt tgggcgaac atgcgggtg tcgtttcta cttgtgccc gcattttag ggactgatg agccctggt ccgcaactc tccactctt cctctgctg ttttgttgc catccaatg aataacatt tcaaatttg aaccaaacg cctgcaaac gtccttaca tcaagtcta agagggttc ctacaggga gttgcggac cagcgcgcc ctaaacttt gttaagcga atttaactc taatctata ataaaattt catccctca gtgttaaag tatattatg gcaaatatt aaactaagc acaataagt atgtaacaa cggttatga tgtatagtt agggtagtc acgacttga aagattcat tatcagagg ttttgatgt aaataattt tgttgattt cttcctaat cgcggttca agcttcgtt tcattgtaa caggatcaa acaacagtt ttcgtttgg tttgcttaa tcgcattgt gtgtctgtt atgaataat aacgcatct aatatataa tttaatggc ataatatca tctcttcct cttgacacc catcgaaac ctcaccact taatgtcac ctaacatat ctcatccaa acttatatc aaattgctt tttatataa tattaataa aatttggaa cttggaata ggatggata aaattgtta gcagttaca ataataaat gttaattgt tgagtacag acgttgtac gaaataatt tcgtctgca gttgaggtt ttaacgacg taagcaatc tattaaccg ggacagcta ccacttcag tcggaaccc ttcaagtgc catgtgtgc ttccgaaat taccccatg agggctctt atgcatctg ttaatggct tgcttacat tttaaaatg tatagaaaa ctagtctaa gtagttcat gttactttc ttaagttgg tactgattt atttatgtc acgctgttg taaaaacat gttaagaac gatgtatga aagtgtgaa aagaaagtg atacaaaaa ca
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