Status
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Gene information
General information
TC010119 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14428740..14446266
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010119
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02874

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    iBeetle screen iB_01651

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010119 RA
      MATLPPRRN PTPTKISKQ HLTPLQILL QMGFPKHRA 
EKALAATGN RGVQLASDW LLAHVNDPL LDDNSPREY 
ILYACPTGP FLEQLQTFW EKSHRECAW NGAHNFTPH 
ITLVSFFKA PDEDALHLA QSLKSVMER QGAMLNEPL 
KLETYTSPN FMGFFVAEE HADYLKRIA MQYVKEVSN 
AIISDTYEH FDALTACFP WCTTTTARC IPRGSRSIS 
LEPHVKSLH LTLAYQFPS THYTHLKSL VDELDPSDS 
AGWELRLYS RDPRVNGKQ VHKVIHPYQ PTECDELDL 
RTGDYVYIS SEALANSPD GWVEGTSWL TGLTGLLPE 
SYTERTAES DAWTLHKKV PLNHITQPE IKYTLPQKA 
LEMEEDASK PSAKDGSES KNGVEDYCF PTSPRIENL 
YENVFKTGP QKEQDKKSR KLYLMRHGE RIDFTFGVW 
IPYCFDESG RYTRKDLNM PSTVPERSH GPAGYTKDT 
PLTNVGLFQ ATTVGDALK EAQLDIAYA YSSPSFRCV 
QTCDALLKG YNKRDEIKI KIEPGLFEW MAWYPDGLP 
DWMTPAELA AAGYNIDLD YQPFVTVTE LKDARETCE 
QYYLRNTFV TRGALNAHL DGNVLFVGH ASTLEVCSR 
ELVGKKPRA AYEMTDVIQ KVAYCSLLQ LAQEGDKWE 
IAELPFPPI THTTNHRFD YKIFLN
    • >TC010119 RB
      MATLPPRRN PTPTKISKQ HLTPLQILL QMGFPKHRA 
EKALAATGN RGVQLASDW LLAHVNDPL LDDNSPREY 
ILYACPTGP FLEQLQTFW EKSHRECAW NGAHNFTPH 
ITLVSFFKA PDEDALHLA QSLKSVMER QGAMLNEPL 
KLETYTSPN FMGFFVAEE HADYLKRIA MQYVKEVSN 
ATISLEPHV KSLHLTLAY QFPSTHYTH LKSLVDELD 
PSDSAGWEL RLYSRDPRV NGKQVHKVI HPYQPTECD 
ELDLRTGDY VYISSEALA NSPDGWVEG TSWLTGLTG 
LLPESYTER TAESDAWTL HKKVPLNHI TQPEIKYTL 
PQKALEMEE DASKPSAKD GSESKNGVE DYCFPTSPR 
IENLYENVF KTGPQKEQD KKSRKLYLM RHGERIDFT 
FGVWIPYCF DESGRYTRK DLNMPSTVP ERSHGPAGY 
TKDTPLTNV GLFQATTVG DALKEAQLD IAYAYSSPS 
FRCVQTCDA LLKGYNKRD EIKIKIEPG LFEWMAWYP 
DGLPDWMTP AELAAAGYN IDLDYQPFV TVTELKDAR 
ETCEQYYLR NTFVTRGAL NAHLDGNVL FVGHASTLE 
VCSRELVGK KPRAAYEMT DVIQKVAYC SLLQLAQEG 
DKWEIAELP FPPITHTTN HRFDYKIFL N
    CDS sequence
    • >TC010119 RA
      atggcaaca ctaccccct aggaggaac cccacacct 
acaaaaata tccaaacag cacctgacc ccgctacaa 
atcctcctc cagatgggg ttccccaaa cacagagcc 
gaaaaggcc ttagctgcc actggtaac aggggcgtg 
caattggcc tccgactgg ttactggcc cacgtaaat 
gacccattg ctggacgat aactctcca agggagtac 
attttatat gcgtgtccc acggggcct ttcctggaa 
cagttacag accttctgg gagaagtcg cacagggaa 
tgcgcctgg aacggggcc cacaatttc acgccgcac 
attacgcta gtttcgttt tttaaggcg cctgatgaa 
gacgcccta catctggcg caaagccta aagtccgta 
atggaaagg cagggcgct atgttgaac gagccccta 
aaactagaa acgtatact tctcccaat tttatgggg 
ttttttgtg gcggaggag cacgctgat tatttaaaa 
aggattgcc atgcaatat gttaaagaa gtttcaaat 
gctattatt agtgacaca tacgaacat ttcgatgct 
ctaacagcc tgttttccg tggtgtacc acaacaact 
gctcgttgc attcctcgg ggaagtaga tctataagt 
cttgaaccc cacgtaaaa tcgctccat ttgacgttg 
gcgtaccaa ttccccagc acccactac acccacctc 
aaatccctc gtggacgag ctcgaccct agcgactcc 
gccggctgg gagttgcgt ctctactcc cgcgatccc 
cgcgtaaac ggtaaacaa gtccacaaa gtgattcac 
ccctaccaa ccaaccgag tgtgacgaa ttggatctt 
cgcacgggc gattacgtc tacataagc agcgaagcc 
ttggccaat tcgcccgac ggttgggtc gagggcact 
tcctggttg acgggactg acgggactc ttgccagag 
agctatacg gaacggacg gcggagtcc gatgcgtgg 
actctgcac aaaaaagtg ccactgaat catatcact 
caaccggaa attaagtac accttaccc caaaaagct 
ttagagatg gaggaggac gcctccaag ccaagtgcc 
aaggatggg agcgaatcg aagaatggg gttgaagat 
tactgcttt cccacgtcg ccgaggatc gagaatttg 
tacgaaaat gtgttcaaa acggggcct caaaaggag 
caagacaaa aaatcccgc aaactctac ctgatgcgt 
cacggggag cgcatagac ttcaccttc ggcgtctgg 
atcccctat tgctttgac gagtctggc cgctacacc 
cggaaagac ctgaatatg ccatccaca gtacctgag 
cgatcgcac ggccccgcc ggctacacc aaggacacc 
cccttgacc aacgtcggt ctcttccag gccaccacc 
gtcggggac gccctcaaa gaagcgcaa ctcgacatc 
gcatacgcc tactcctcg ccctcgttc cggtgcgtc 
cagacctgc gacgccctc ctcaaaggc tacaacaaa 
cgcgacgaa atcaaaatc aaaatcgag cctggactt 
ttcgaatgg atggcctgg tatccggac gggctgccc 
gactggatg acaccggcc gaactggcc gcagctggc 
tacaacata gacctggac tatcagccg ttcgtgacc 
gtaacggag ctgaaagac gccagggag acttgcgag 
cagtattat ttgcgcaac actttcgtc acgagaggg 
gctctcaat gcgcacctc gacggaaac gtgctgttt 
gtgggccac gcgtccact ttggaagtg tgttcgagg 
gaattggtc gggaagaag ccgcgggct gcgtatgaa 
atgaccgat gttatacag aaagtggcc tattgtagt 
ttactgcaa ctggcgcaa gagggcgac aagtgggag 
attgccgag ctgcccttc ccgcccatc acacacaca 
accaatcat aggttcgat tataagatt ttcctgaat 
taa
    • >TC010119 RB
      atggcaaca ctaccccct aggaggaac cccacacct 
acaaaaata tccaaacag cacctgacc ccgctacaa 
atcctcctc cagatgggg ttccccaaa cacagagcc 
gaaaaggcc ttagctgcc actggtaac aggggcgtg 
caattggcc tccgactgg ttactggcc cacgtaaat 
gacccattg ctggacgat aactctcca agggagtac 
attttatat gcgtgtccc acggggcct ttcctggaa 
cagttacag accttctgg gagaagtcg cacagggaa 
tgcgcctgg aacggggcc cacaatttc acgccgcac 
attacgcta gtttcgttt tttaaggcg cctgatgaa 
gacgcccta catctggcg caaagccta aagtccgta 
atggaaagg cagggcgct atgttgaac gagccccta 
aaactagaa acgtatact tctcccaat tttatgggg 
ttttttgtg gcggaggag cacgctgat tatttaaaa 
aggattgcc atgcaatat gttaaagaa gtttcaaat 
gctactata agtcttgaa ccccacgta aaatcgctc 
catttgacg ttggcgtac caattcccc agcacccac 
tacacccac ctcaaatcc ctcgtggac gagctcgac 
cctagcgac tccgccggc tgggagttg cgtctctac 
tcccgcgat ccccgcgta aacggtaaa caagtccac 
aaagtgatt cacccctac caaccaacc gagtgtgac 
gaattggat cttcgcacg ggcgattac gtctacata 
agcagcgaa gccttggcc aattcgccc gacggttgg 
gtcgagggc acttcctgg ttgacggga ctgacggga 
ctcttgcca gagagctat acggaacgg acggcggag 
tccgatgcg tggactctg cacaaaaaa gtgccactg 
aatcatatc actcaaccg gaaattaag tacacctta 
ccccaaaaa gctttagag atggaggag gacgcctcc 
aagccaagt gccaaggat gggagcgaa tcgaagaat 
ggggttgaa gattactgc tttcccacg tcgccgagg 
atcgagaat ttgtacgaa aatgtgttc aaaacgggg 
cctcaaaag gagcaagac aaaaaatcc cgcaaactc 
tacctgatg cgtcacggg gagcgcata gacttcacc 
ttcggcgtc tggatcccc tattgcttt gacgagtct 
ggccgctac acccggaaa gacctgaat atgccatcc 
acagtacct gagcgatcg cacggcccc gccggctac 
accaaggac acccccttg accaacgtc ggtctcttc 
caggccacc accgtcggg gacgccctc aaagaagcg 
caactcgac atcgcatac gcctactcc tcgccctcg 
ttccggtgc gtccagacc tgcgacgcc ctcctcaaa 
ggctacaac aaacgcgac gaaatcaaa atcaaaatc 
gagcctgga cttttcgaa tggatggcc tggtatccg 
gacgggctg cccgactgg atgacaccg gccgaactg 
gccgcagct ggctacaac atagacctg gactatcag 
ccgttcgtg accgtaacg gagctgaaa gacgccagg 
gagacttgc gagcagtat tatttgcgc aacactttc 
gtcacgaga ggggctctc aatgcgcac ctcgacgga 
aacgtgctg tttgtgggc cacgcgtcc actttggaa 
gtgtgttcg agggaattg gtcgggaag aagccgcgg 
gctgcgtat gaaatgacc gatgttata cagaaagtg 
gcctattgt agtttactg caactggcg caagagggc 
gacaagtgg gagattgcc gagctgccc ttcccgccc 
atcacacac acaaccaat cataggttc gattataag 
attttcctg aattaa
    mRNA sequence
    • >TC010119 RA
      cctcgatca aatttcttt ccctcagga tcaatatct 
ttcacgttg aatatatct tcaaacagg acacctgcc 
atcgtcgaa taacgtgta aataataaa aaattaaca 
cactttaat actttctta tgaaaatgg gcaaatttt 
gacagacat caacacttg acagtgttc caaccagtg 
ccgccatct gaaaggcca attccccaa acaaaacca 
aaacaaaaa cgtcactgt ttaaatgtc aaaggtcca 
ctagaagtg tcaacacca aactttaac ctagttgaa 
attaataat ttattaaat gaaaatggc aacactacc 
ccctaggag gaaccccac acctacaaa aatatccaa 
acagcacct gaccccgct acaaatcct cctccagat 
ggggttccc caaacacag agccgaaaa ggccttagc 
tgccactgg taacagggg cgtgcaatt ggcctccga 
ctggttact ggcccacgt aaatgaccc attgctgga 
cgataactc tccaaggga gtacatttt atatgcgtg 
tcccacggg gcctttcct ggaacagtt acagacctt 
ctgggagaa gtcgcacag ggaatgcgc ctggaacgg 
ggcccacaa tttcacgcc gcacattac gctagtttc 
gttttttaa ggcgcctga tgaagacgc cctacatct 
ggcgcaaag cctaaagtc cgtaatgga aaggcaggg 
cgctatgtt gaacgagcc cctaaaact agaaacgta 
tacttctcc caattttat ggggttttt tgtggcgga 
ggagcacgc tgattattt aaaaaggat tgccatgca 
atatgttaa agaagtttc aaatgctat tattagtga 
cacatacga acatttcga tgctctaac agcctgttt 
tccgtggtg taccacaac aactgctcg ttgcattcc 
tcggggaag tagatctat aagtcttga accccacgt 
aaaatcgct ccatttgac gttggcgta ccaattccc 
cagcaccca ctacaccca cctcaaatc cctcgtgga 
cgagctcga ccctagcga ctccgccgg ctgggagtt 
gcgtctcta ctcccgcga tccccgcgt aaacggtaa 
acaagtcca caaagtgat tcaccccta ccaaccaac 
cgagtgtga cgaattgga tcttcgcac gggcgatta 
cgtctacat aagcagcga agccttggc caattcgcc 
cgacggttg ggtcgaggg cacttcctg gttgacggg 
actgacggg actcttgcc agagagcta tacggaacg 
gacggcgga gtccgatgc gtggactct gcacaaaaa 
agtgccact gaatcatat cactcaacc ggaaattaa 
gtacacctt accccaaaa agctttaga gatggagga 
ggacgcctc caagccaag tgccaagga tgggagcga 
atcgaagaa tggggttga agattactg ctttcccac 
gtcgccgag gatcgagaa tttgtacga aaatgtgtt 
caaaacggg gcctcaaaa ggagcaaga caaaaaatc 
ccgcaaact ctacctgat gcgtcacgg ggagcgcat 
agacttcac cttcggcgt ctggatccc ctattgctt 
tgacgagtc tggccgcta cacccggaa agacctgaa 
tatgccatc cacagtacc tgagcgatc gcacggccc 
cgccggcta caccaagga caccccctt gaccaacgt 
cggtctctt ccaggccac caccgtcgg ggacgccct 
caaagaagc gcaactcga catcgcata cgcctactc 
ctcgccctc gttccggtg cgtccagac ctgcgacgc 
cctcctcaa aggctacaa caaacgcga cgaaatcaa 
aatcaaaat cgagcctgg acttttcga atggatggc 
ctggtatcc ggacgggct gcccgactg gatgacacc 
ggccgaact ggccgcagc tggctacaa catagacct 
ggactatca gccgttcgt gaccgtaac ggagctgaa 
agacgccag ggagacttg cgagcagta ttatttgcg 
caacacttt cgtcacgag aggggctct caatgcgca 
cctcgacgg aaacgtgct gtttgtggg ccacgcgtc 
cactttgga agtgtgttc gagggaatt ggtcgggaa 
gaagccgcg ggctgcgta tgaaatgac cgatgttat 
acagaaagt ggcctattg tagtttact gcaactggc 
gcaagaggg cgacaagtg ggagattgc cgagctgcc 
cttcccgcc catcacaca cacaaccaa tcataggtt 
cgattataa gattttcct gaattaatt gaaacggtg 
ccctccatc gtggagcaa atcttagaa ttcaaaatt 
ggcgaaacg tccaatggg tggttcacc aattttgac 
taggatttg gccacaatg taatgtaat ccatttacc 
aaagaacgc ctgcatatt ttatttttg ctatatttt 
gttgcatag acttgatac aataagaat aaagaaagc 
attaattgt tgtggaaaa ccgatttac gggggactc 
agatattaa ggggcctaa ctaaaaatc tgagaaaca 
aatcaaaac ttatggtat atttagtct gttttgctc 
gatttagtt caaaaataa gccaaaaaa ttaccaaat 
ttaaacaag acagtgtaa tttttacct ctttaccaa 
aaaatcacg aaattaaat tatatttta taaaatttt 
gtttaaaca agtcaaaat cgaccgaaa atggtgtta 
tttttgaca gttttgggc gctccttca gcgagatat 
ttttaaagt agagcgaaa gtttaggaa agtaagtca 
aaattttca atatttttt cctttttca accattttg 
ggacgcgat tattttgag gaattttgt ttaagataa 
gctgagtca aagtgctag taacacgtt ttttgtcca 
aaagcgcat aattttgtt taaaattac attaaaaat 
gattaaatt gggtcacaa aaagtgtta tttttacct 
ttttgctct ttttaacct aaaagctct atacctgta 
aatgcttcg aagaatcaa aatacaaaa atgtcatta 
ttttgcctt tcggcgttt tagtatttg aaaatattt 
ttcgtccaa agcccgaaa ttgccgaat tgggccgaa 
aaagtcgtt ttacgagcg aaatttaca gatttgttg 
aattgtatt aaaagtgaa attttaacc tttttctag 
ttcttaaac acttatttt aaataaaaa acttcaaga 
agtttcatt aaatcggaa aaaatcagt aaatgaggt 
caaaattgt catttttct cgaattctt agaacagaa 
aggtaatca tttttatat tgaattgta ttaaatttc 
tttcttttt aatatttat tgtaagggt cccttttcc 
gcactgatc atctcagac aatatgcat attttttca 
tat
    • >TC010119 RB
      cctcgatca aatttcttt ccctcagga tcaatatct 
ttcacgttg aatatatct tcaaacagg acacctgcc 
atcgtcgaa taacgtgta aataataaa aaattaaca 
cactttaat actttctta tgaaaatgg gcaaatttt 
gacagacat caacacttg acagtgttc caaccagtg 
ccgccatct gaaaggcca attccccaa acaaaacca 
aaacaaaaa cgtcactgt ttaaatgtc aaaggtcca 
ctagaagtg tcaacacca aactttaac ctagttgaa 
attaataat ttattaaat gaaaatggc aacactacc 
ccctaggag gaaccccac acctacaaa aatatccaa 
acagcacct gaccccgct acaaatcct cctccagat 
ggggttccc caaacacag agccgaaaa ggccttagc 
tgccactgg taacagggg cgtgcaatt ggcctccga 
ctggttact ggcccacgt aaatgaccc attgctgga 
cgataactc tccaaggga gtacatttt atatgcgtg 
tcccacggg gcctttcct ggaacagtt acagacctt 
ctgggagaa gtcgcacag ggaatgcgc ctggaacgg 
ggcccacaa tttcacgcc gcacattac gctagtttc 
gttttttaa ggcgcctga tgaagacgc cctacatct 
ggcgcaaag cctaaagtc cgtaatgga aaggcaggg 
cgctatgtt gaacgagcc cctaaaact agaaacgta 
tacttctcc caattttat ggggttttt tgtggcgga 
ggagcacgc tgattattt aaaaaggat tgccatgca 
atatgttaa agaagtttc aaatgctac tataagtct 
tgaacccca cgtaaaatc gctccattt gacgttggc 
gtaccaatt ccccagcac ccactacac ccacctcaa 
atccctcgt ggacgagct cgaccctag cgactccgc 
cggctggga gttgcgtct ctactcccg cgatccccg 
cgtaaacgg taaacaagt ccacaaagt gattcaccc 
ctaccaacc aaccgagtg tgacgaatt ggatcttcg 
cacgggcga ttacgtcta cataagcag cgaagcctt 
ggccaattc gcccgacgg ttgggtcga gggcacttc 
ctggttgac gggactgac gggactctt gccagagag 
ctatacgga acggacggc ggagtccga tgcgtggac 
tctgcacaa aaaagtgcc actgaatca tatcactca 
accggaaat taagtacac cttacccca aaaagcttt 
agagatgga ggaggacgc ctccaagcc aagtgccaa 
ggatgggag cgaatcgaa gaatggggt tgaagatta 
ctgctttcc cacgtcgcc gaggatcga gaatttgta 
cgaaaatgt gttcaaaac ggggcctca aaaggagca 
agacaaaaa atcccgcaa actctacct gatgcgtca 
cggggagcg catagactt caccttcgg cgtctggat 
cccctattg ctttgacga gtctggccg ctacacccg 
gaaagacct gaatatgcc atccacagt acctgagcg 
atcgcacgg ccccgccgg ctacaccaa ggacacccc 
cttgaccaa cgtcggtct cttccaggc caccaccgt 
cggggacgc cctcaaaga agcgcaact cgacatcgc 
atacgccta ctcctcgcc ctcgttccg gtgcgtcca 
gacctgcga cgccctcct caaaggcta caacaaacg 
cgacgaaat caaaatcaa aatcgagcc tggactttt 
cgaatggat ggcctggta tccggacgg gctgcccga 
ctggatgac accggccga actggccgc agctggcta 
caacataga cctggacta tcagccgtt cgtgaccgt 
aacggagct gaaagacgc cagggagac ttgcgagca 
gtattattt gcgcaacac tttcgtcac gagaggggc 
tctcaatgc gcacctcga cggaaacgt gctgtttgt 
gggccacgc gtccacttt ggaagtgtg ttcgaggga 
attggtcgg gaagaagcc gcgggctgc gtatgaaat 
gaccgatgt tatacagaa agtggccta ttgtagttt 
actgcaact ggcgcaaga gggcgacaa gtgggagat 
tgccgagct gcccttccc gcccatcac acacacaac 
caatcatag gttcgatta taagatttt cctgaatta 
attgaaacg gtgccctcc atcgtggag caaatctta 
gaattcaaa attggcgaa acgtccaat gggtggttc 
accaatttt gactaggat ttggccaca atgtaatgt 
aatccattt accaaagaa cgcctgcat attttattt 
ttgctatat tttgttgca tagacttga tacaataag 
aataaagaa agcattaat tgttgtgga aaaccgatt 
tacggggga ctcagatat taaggggcc taactaaaa 
atctgagaa acaaatcaa aacttatgg tatatttag 
tctgttttg ctcgattta gttcaaaaa taagccaaa 
aaattacca aatttaaac aagacagtg taattttta 
cctctttac caaaaaatc acgaaatta aattatatt 
ttataaaat tttgtttaa acaagtcaa aatcgaccg 
aaaatggtg ttatttttg acagttttg ggcgctcct 
tcagcgaga tatttttaa agtagagcg aaagtttag 
gaaagtaag tcaaaattt tcaatattt tttcctttt 
tcaaccatt ttgggacgc gattatttt gaggaattt 
tgtttaaga taagctgag tcaaagtgc tagtaacac 
gttttttgt ccaaaagcg cataatttt gtttaaaat 
tacattaaa aatgattaa attgggtca caaaaagtg 
ttattttta cctttttgc tctttttaa cctaaaagc 
tctatacct gtaaatgct tcgaagaat caaaataca 
aaaatgtca ttattttgc ctttcggcg ttttagtat 
ttgaaaata tttttcgtc caaagcccg aaattgccg 
aattgggcc gaaaaagtc gttttacga gcgaaattt 
acagatttg ttgaattgt attaaaagt gaaatttta 
acctttttc tagttctta aacacttat tttaaataa 
aaaacttca agaagtttc attaaatcg gaaaaaatc 
agtaaatga ggtcaaaat tgtcatttt tctcgaatt 
cttagaaca gaaaggtaa tcattttta tattgaatt 
gtattaaat ttctttctt tttaatatt tattgtaag 
ggtcccttt tccgcactg atcatctca gacaatatg 
catattttt tcatat
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