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Gene information
General information
TC010119 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14428740..14446266
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010119
    iBeetle screen iB_02874

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    iBeetle screen iB_01651

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010119 RA
      MATLPPRRN PTPTKISKQ HLTPLQILL QMGFPKHRA 
      EKALAATGN RGVQLASDW LLAHVNDPL LDDNSPREY 
      ILYACPTGP FLEQLQTFW EKSHRECAW NGAHNFTPH 
      ITLVSFFKA PDEDALHLA QSLKSVMER QGAMLNEPL 
      KLETYTSPN FMGFFVAEE HADYLKRIA MQYVKEVSN 
      AIISDTYEH FDALTACFP WCTTTTARC IPRGSRSIS 
      LEPHVKSLH LTLAYQFPS THYTHLKSL VDELDPSDS 
      AGWELRLYS RDPRVNGKQ VHKVIHPYQ PTECDELDL 
      RTGDYVYIS SEALANSPD GWVEGTSWL TGLTGLLPE 
      SYTERTAES DAWTLHKKV PLNHITQPE IKYTLPQKA 
      LEMEEDASK PSAKDGSES KNGVEDYCF PTSPRIENL 
      YENVFKTGP QKEQDKKSR KLYLMRHGE RIDFTFGVW 
      IPYCFDESG RYTRKDLNM PSTVPERSH GPAGYTKDT 
      PLTNVGLFQ ATTVGDALK EAQLDIAYA YSSPSFRCV 
      QTCDALLKG YNKRDEIKI KIEPGLFEW MAWYPDGLP 
      DWMTPAELA AAGYNIDLD YQPFVTVTE LKDARETCE 
      QYYLRNTFV TRGALNAHL DGNVLFVGH ASTLEVCSR 
      ELVGKKPRA AYEMTDVIQ KVAYCSLLQ LAQEGDKWE 
      IAELPFPPI THTTNHRFD YKIFLN
    • >TC010119 RB
      MATLPPRRN PTPTKISKQ HLTPLQILL QMGFPKHRA 
      EKALAATGN RGVQLASDW LLAHVNDPL LDDNSPREY 
      ILYACPTGP FLEQLQTFW EKSHRECAW NGAHNFTPH 
      ITLVSFFKA PDEDALHLA QSLKSVMER QGAMLNEPL 
      KLETYTSPN FMGFFVAEE HADYLKRIA MQYVKEVSN 
      ATISLEPHV KSLHLTLAY QFPSTHYTH LKSLVDELD 
      PSDSAGWEL RLYSRDPRV NGKQVHKVI HPYQPTECD 
      ELDLRTGDY VYISSEALA NSPDGWVEG TSWLTGLTG 
      LLPESYTER TAESDAWTL HKKVPLNHI TQPEIKYTL 
      PQKALEMEE DASKPSAKD GSESKNGVE DYCFPTSPR 
      IENLYENVF KTGPQKEQD KKSRKLYLM RHGERIDFT 
      FGVWIPYCF DESGRYTRK DLNMPSTVP ERSHGPAGY 
      TKDTPLTNV GLFQATTVG DALKEAQLD IAYAYSSPS 
      FRCVQTCDA LLKGYNKRD EIKIKIEPG LFEWMAWYP 
      DGLPDWMTP AELAAAGYN IDLDYQPFV TVTELKDAR 
      ETCEQYYLR NTFVTRGAL NAHLDGNVL FVGHASTLE 
      VCSRELVGK KPRAAYEMT DVIQKVAYC SLLQLAQEG 
      DKWEIAELP FPPITHTTN HRFDYKIFL N
    CDS sequence
    • >TC010119 RA
      atggcaaca ctaccccct aggaggaac cccacacct 
      acaaaaata tccaaacag cacctgacc ccgctacaa 
      atcctcctc cagatgggg ttccccaaa cacagagcc 
      gaaaaggcc ttagctgcc actggtaac aggggcgtg 
      caattggcc tccgactgg ttactggcc cacgtaaat 
      gacccattg ctggacgat aactctcca agggagtac 
      attttatat gcgtgtccc acggggcct ttcctggaa 
      cagttacag accttctgg gagaagtcg cacagggaa 
      tgcgcctgg aacggggcc cacaatttc acgccgcac 
      attacgcta gtttcgttt tttaaggcg cctgatgaa 
      gacgcccta catctggcg caaagccta aagtccgta 
      atggaaagg cagggcgct atgttgaac gagccccta 
      aaactagaa acgtatact tctcccaat tttatgggg 
      ttttttgtg gcggaggag cacgctgat tatttaaaa 
      aggattgcc atgcaatat gttaaagaa gtttcaaat 
      gctattatt agtgacaca tacgaacat ttcgatgct 
      ctaacagcc tgttttccg tggtgtacc acaacaact 
      gctcgttgc attcctcgg ggaagtaga tctataagt 
      cttgaaccc cacgtaaaa tcgctccat ttgacgttg 
      gcgtaccaa ttccccagc acccactac acccacctc 
      aaatccctc gtggacgag ctcgaccct agcgactcc 
      gccggctgg gagttgcgt ctctactcc cgcgatccc 
      cgcgtaaac ggtaaacaa gtccacaaa gtgattcac 
      ccctaccaa ccaaccgag tgtgacgaa ttggatctt 
      cgcacgggc gattacgtc tacataagc agcgaagcc 
      ttggccaat tcgcccgac ggttgggtc gagggcact 
      tcctggttg acgggactg acgggactc ttgccagag 
      agctatacg gaacggacg gcggagtcc gatgcgtgg 
      actctgcac aaaaaagtg ccactgaat catatcact 
      caaccggaa attaagtac accttaccc caaaaagct 
      ttagagatg gaggaggac gcctccaag ccaagtgcc 
      aaggatggg agcgaatcg aagaatggg gttgaagat 
      tactgcttt cccacgtcg ccgaggatc gagaatttg 
      tacgaaaat gtgttcaaa acggggcct caaaaggag 
      caagacaaa aaatcccgc aaactctac ctgatgcgt 
      cacggggag cgcatagac ttcaccttc ggcgtctgg 
      atcccctat tgctttgac gagtctggc cgctacacc 
      cggaaagac ctgaatatg ccatccaca gtacctgag 
      cgatcgcac ggccccgcc ggctacacc aaggacacc 
      cccttgacc aacgtcggt ctcttccag gccaccacc 
      gtcggggac gccctcaaa gaagcgcaa ctcgacatc 
      gcatacgcc tactcctcg ccctcgttc cggtgcgtc 
      cagacctgc gacgccctc ctcaaaggc tacaacaaa 
      cgcgacgaa atcaaaatc aaaatcgag cctggactt 
      ttcgaatgg atggcctgg tatccggac gggctgccc 
      gactggatg acaccggcc gaactggcc gcagctggc 
      tacaacata gacctggac tatcagccg ttcgtgacc 
      gtaacggag ctgaaagac gccagggag acttgcgag 
      cagtattat ttgcgcaac actttcgtc acgagaggg 
      gctctcaat gcgcacctc gacggaaac gtgctgttt 
      gtgggccac gcgtccact ttggaagtg tgttcgagg 
      gaattggtc gggaagaag ccgcgggct gcgtatgaa 
      atgaccgat gttatacag aaagtggcc tattgtagt 
      ttactgcaa ctggcgcaa gagggcgac aagtgggag 
      attgccgag ctgcccttc ccgcccatc acacacaca 
      accaatcat aggttcgat tataagatt ttcctgaat 
      taa
    • >TC010119 RB
      atggcaaca ctaccccct aggaggaac cccacacct 
      acaaaaata tccaaacag cacctgacc ccgctacaa 
      atcctcctc cagatgggg ttccccaaa cacagagcc 
      gaaaaggcc ttagctgcc actggtaac aggggcgtg 
      caattggcc tccgactgg ttactggcc cacgtaaat 
      gacccattg ctggacgat aactctcca agggagtac 
      attttatat gcgtgtccc acggggcct ttcctggaa 
      cagttacag accttctgg gagaagtcg cacagggaa 
      tgcgcctgg aacggggcc cacaatttc acgccgcac 
      attacgcta gtttcgttt tttaaggcg cctgatgaa 
      gacgcccta catctggcg caaagccta aagtccgta 
      atggaaagg cagggcgct atgttgaac gagccccta 
      aaactagaa acgtatact tctcccaat tttatgggg 
      ttttttgtg gcggaggag cacgctgat tatttaaaa 
      aggattgcc atgcaatat gttaaagaa gtttcaaat 
      gctactata agtcttgaa ccccacgta aaatcgctc 
      catttgacg ttggcgtac caattcccc agcacccac 
      tacacccac ctcaaatcc ctcgtggac gagctcgac 
      cctagcgac tccgccggc tgggagttg cgtctctac 
      tcccgcgat ccccgcgta aacggtaaa caagtccac 
      aaagtgatt cacccctac caaccaacc gagtgtgac 
      gaattggat cttcgcacg ggcgattac gtctacata 
      agcagcgaa gccttggcc aattcgccc gacggttgg 
      gtcgagggc acttcctgg ttgacggga ctgacggga 
      ctcttgcca gagagctat acggaacgg acggcggag 
      tccgatgcg tggactctg cacaaaaaa gtgccactg 
      aatcatatc actcaaccg gaaattaag tacacctta 
      ccccaaaaa gctttagag atggaggag gacgcctcc 
      aagccaagt gccaaggat gggagcgaa tcgaagaat 
      ggggttgaa gattactgc tttcccacg tcgccgagg 
      atcgagaat ttgtacgaa aatgtgttc aaaacgggg 
      cctcaaaag gagcaagac aaaaaatcc cgcaaactc 
      tacctgatg cgtcacggg gagcgcata gacttcacc 
      ttcggcgtc tggatcccc tattgcttt gacgagtct 
      ggccgctac acccggaaa gacctgaat atgccatcc 
      acagtacct gagcgatcg cacggcccc gccggctac 
      accaaggac acccccttg accaacgtc ggtctcttc 
      caggccacc accgtcggg gacgccctc aaagaagcg 
      caactcgac atcgcatac gcctactcc tcgccctcg 
      ttccggtgc gtccagacc tgcgacgcc ctcctcaaa 
      ggctacaac aaacgcgac gaaatcaaa atcaaaatc 
      gagcctgga cttttcgaa tggatggcc tggtatccg 
      gacgggctg cccgactgg atgacaccg gccgaactg 
      gccgcagct ggctacaac atagacctg gactatcag 
      ccgttcgtg accgtaacg gagctgaaa gacgccagg 
      gagacttgc gagcagtat tatttgcgc aacactttc 
      gtcacgaga ggggctctc aatgcgcac ctcgacgga 
      aacgtgctg tttgtgggc cacgcgtcc actttggaa 
      gtgtgttcg agggaattg gtcgggaag aagccgcgg 
      gctgcgtat gaaatgacc gatgttata cagaaagtg 
      gcctattgt agtttactg caactggcg caagagggc 
      gacaagtgg gagattgcc gagctgccc ttcccgccc 
      atcacacac acaaccaat cataggttc gattataag 
      attttcctg aattaa
    mRNA sequence
    • >TC010119 RA
      cctcgatca aatttcttt ccctcagga tcaatatct 
      ttcacgttg aatatatct tcaaacagg acacctgcc 
      atcgtcgaa taacgtgta aataataaa aaattaaca 
      cactttaat actttctta tgaaaatgg gcaaatttt 
      gacagacat caacacttg acagtgttc caaccagtg 
      ccgccatct gaaaggcca attccccaa acaaaacca 
      aaacaaaaa cgtcactgt ttaaatgtc aaaggtcca 
      ctagaagtg tcaacacca aactttaac ctagttgaa 
      attaataat ttattaaat gaaaatggc aacactacc 
      ccctaggag gaaccccac acctacaaa aatatccaa 
      acagcacct gaccccgct acaaatcct cctccagat 
      ggggttccc caaacacag agccgaaaa ggccttagc 
      tgccactgg taacagggg cgtgcaatt ggcctccga 
      ctggttact ggcccacgt aaatgaccc attgctgga 
      cgataactc tccaaggga gtacatttt atatgcgtg 
      tcccacggg gcctttcct ggaacagtt acagacctt 
      ctgggagaa gtcgcacag ggaatgcgc ctggaacgg 
      ggcccacaa tttcacgcc gcacattac gctagtttc 
      gttttttaa ggcgcctga tgaagacgc cctacatct 
      ggcgcaaag cctaaagtc cgtaatgga aaggcaggg 
      cgctatgtt gaacgagcc cctaaaact agaaacgta 
      tacttctcc caattttat ggggttttt tgtggcgga 
      ggagcacgc tgattattt aaaaaggat tgccatgca 
      atatgttaa agaagtttc aaatgctat tattagtga 
      cacatacga acatttcga tgctctaac agcctgttt 
      tccgtggtg taccacaac aactgctcg ttgcattcc 
      tcggggaag tagatctat aagtcttga accccacgt 
      aaaatcgct ccatttgac gttggcgta ccaattccc 
      cagcaccca ctacaccca cctcaaatc cctcgtgga 
      cgagctcga ccctagcga ctccgccgg ctgggagtt 
      gcgtctcta ctcccgcga tccccgcgt aaacggtaa 
      acaagtcca caaagtgat tcaccccta ccaaccaac 
      cgagtgtga cgaattgga tcttcgcac gggcgatta 
      cgtctacat aagcagcga agccttggc caattcgcc 
      cgacggttg ggtcgaggg cacttcctg gttgacggg 
      actgacggg actcttgcc agagagcta tacggaacg 
      gacggcgga gtccgatgc gtggactct gcacaaaaa 
      agtgccact gaatcatat cactcaacc ggaaattaa 
      gtacacctt accccaaaa agctttaga gatggagga 
      ggacgcctc caagccaag tgccaagga tgggagcga 
      atcgaagaa tggggttga agattactg ctttcccac 
      gtcgccgag gatcgagaa tttgtacga aaatgtgtt 
      caaaacggg gcctcaaaa ggagcaaga caaaaaatc 
      ccgcaaact ctacctgat gcgtcacgg ggagcgcat 
      agacttcac cttcggcgt ctggatccc ctattgctt 
      tgacgagtc tggccgcta cacccggaa agacctgaa 
      tatgccatc cacagtacc tgagcgatc gcacggccc 
      cgccggcta caccaagga caccccctt gaccaacgt 
      cggtctctt ccaggccac caccgtcgg ggacgccct 
      caaagaagc gcaactcga catcgcata cgcctactc 
      ctcgccctc gttccggtg cgtccagac ctgcgacgc 
      cctcctcaa aggctacaa caaacgcga cgaaatcaa 
      aatcaaaat cgagcctgg acttttcga atggatggc 
      ctggtatcc ggacgggct gcccgactg gatgacacc 
      ggccgaact ggccgcagc tggctacaa catagacct 
      ggactatca gccgttcgt gaccgtaac ggagctgaa 
      agacgccag ggagacttg cgagcagta ttatttgcg 
      caacacttt cgtcacgag aggggctct caatgcgca 
      cctcgacgg aaacgtgct gtttgtggg ccacgcgtc 
      cactttgga agtgtgttc gagggaatt ggtcgggaa 
      gaagccgcg ggctgcgta tgaaatgac cgatgttat 
      acagaaagt ggcctattg tagtttact gcaactggc 
      gcaagaggg cgacaagtg ggagattgc cgagctgcc 
      cttcccgcc catcacaca cacaaccaa tcataggtt 
      cgattataa gattttcct gaattaatt gaaacggtg 
      ccctccatc gtggagcaa atcttagaa ttcaaaatt 
      ggcgaaacg tccaatggg tggttcacc aattttgac 
      taggatttg gccacaatg taatgtaat ccatttacc 
      aaagaacgc ctgcatatt ttatttttg ctatatttt 
      gttgcatag acttgatac aataagaat aaagaaagc 
      attaattgt tgtggaaaa ccgatttac gggggactc 
      agatattaa ggggcctaa ctaaaaatc tgagaaaca 
      aatcaaaac ttatggtat atttagtct gttttgctc 
      gatttagtt caaaaataa gccaaaaaa ttaccaaat 
      ttaaacaag acagtgtaa tttttacct ctttaccaa 
      aaaatcacg aaattaaat tatatttta taaaatttt 
      gtttaaaca agtcaaaat cgaccgaaa atggtgtta 
      tttttgaca gttttgggc gctccttca gcgagatat 
      ttttaaagt agagcgaaa gtttaggaa agtaagtca 
      aaattttca atatttttt cctttttca accattttg 
      ggacgcgat tattttgag gaattttgt ttaagataa 
      gctgagtca aagtgctag taacacgtt ttttgtcca 
      aaagcgcat aattttgtt taaaattac attaaaaat 
      gattaaatt gggtcacaa aaagtgtta tttttacct 
      ttttgctct ttttaacct aaaagctct atacctgta 
      aatgcttcg aagaatcaa aatacaaaa atgtcatta 
      ttttgcctt tcggcgttt tagtatttg aaaatattt 
      ttcgtccaa agcccgaaa ttgccgaat tgggccgaa 
      aaagtcgtt ttacgagcg aaatttaca gatttgttg 
      aattgtatt aaaagtgaa attttaacc tttttctag 
      ttcttaaac acttatttt aaataaaaa acttcaaga 
      agtttcatt aaatcggaa aaaatcagt aaatgaggt 
      caaaattgt catttttct cgaattctt agaacagaa 
      aggtaatca tttttatat tgaattgta ttaaatttc 
      tttcttttt aatatttat tgtaagggt cccttttcc 
      gcactgatc atctcagac aatatgcat attttttca 
      tat
    • >TC010119 RB
      cctcgatca aatttcttt ccctcagga tcaatatct 
      ttcacgttg aatatatct tcaaacagg acacctgcc 
      atcgtcgaa taacgtgta aataataaa aaattaaca 
      cactttaat actttctta tgaaaatgg gcaaatttt 
      gacagacat caacacttg acagtgttc caaccagtg 
      ccgccatct gaaaggcca attccccaa acaaaacca 
      aaacaaaaa cgtcactgt ttaaatgtc aaaggtcca 
      ctagaagtg tcaacacca aactttaac ctagttgaa 
      attaataat ttattaaat gaaaatggc aacactacc 
      ccctaggag gaaccccac acctacaaa aatatccaa 
      acagcacct gaccccgct acaaatcct cctccagat 
      ggggttccc caaacacag agccgaaaa ggccttagc 
      tgccactgg taacagggg cgtgcaatt ggcctccga 
      ctggttact ggcccacgt aaatgaccc attgctgga 
      cgataactc tccaaggga gtacatttt atatgcgtg 
      tcccacggg gcctttcct ggaacagtt acagacctt 
      ctgggagaa gtcgcacag ggaatgcgc ctggaacgg 
      ggcccacaa tttcacgcc gcacattac gctagtttc 
      gttttttaa ggcgcctga tgaagacgc cctacatct 
      ggcgcaaag cctaaagtc cgtaatgga aaggcaggg 
      cgctatgtt gaacgagcc cctaaaact agaaacgta 
      tacttctcc caattttat ggggttttt tgtggcgga 
      ggagcacgc tgattattt aaaaaggat tgccatgca 
      atatgttaa agaagtttc aaatgctac tataagtct 
      tgaacccca cgtaaaatc gctccattt gacgttggc 
      gtaccaatt ccccagcac ccactacac ccacctcaa 
      atccctcgt ggacgagct cgaccctag cgactccgc 
      cggctggga gttgcgtct ctactcccg cgatccccg 
      cgtaaacgg taaacaagt ccacaaagt gattcaccc 
      ctaccaacc aaccgagtg tgacgaatt ggatcttcg 
      cacgggcga ttacgtcta cataagcag cgaagcctt 
      ggccaattc gcccgacgg ttgggtcga gggcacttc 
      ctggttgac gggactgac gggactctt gccagagag 
      ctatacgga acggacggc ggagtccga tgcgtggac 
      tctgcacaa aaaagtgcc actgaatca tatcactca 
      accggaaat taagtacac cttacccca aaaagcttt 
      agagatgga ggaggacgc ctccaagcc aagtgccaa 
      ggatgggag cgaatcgaa gaatggggt tgaagatta 
      ctgctttcc cacgtcgcc gaggatcga gaatttgta 
      cgaaaatgt gttcaaaac ggggcctca aaaggagca 
      agacaaaaa atcccgcaa actctacct gatgcgtca 
      cggggagcg catagactt caccttcgg cgtctggat 
      cccctattg ctttgacga gtctggccg ctacacccg 
      gaaagacct gaatatgcc atccacagt acctgagcg 
      atcgcacgg ccccgccgg ctacaccaa ggacacccc 
      cttgaccaa cgtcggtct cttccaggc caccaccgt 
      cggggacgc cctcaaaga agcgcaact cgacatcgc 
      atacgccta ctcctcgcc ctcgttccg gtgcgtcca 
      gacctgcga cgccctcct caaaggcta caacaaacg 
      cgacgaaat caaaatcaa aatcgagcc tggactttt 
      cgaatggat ggcctggta tccggacgg gctgcccga 
      ctggatgac accggccga actggccgc agctggcta 
      caacataga cctggacta tcagccgtt cgtgaccgt 
      aacggagct gaaagacgc cagggagac ttgcgagca 
      gtattattt gcgcaacac tttcgtcac gagaggggc 
      tctcaatgc gcacctcga cggaaacgt gctgtttgt 
      gggccacgc gtccacttt ggaagtgtg ttcgaggga 
      attggtcgg gaagaagcc gcgggctgc gtatgaaat 
      gaccgatgt tatacagaa agtggccta ttgtagttt 
      actgcaact ggcgcaaga gggcgacaa gtgggagat 
      tgccgagct gcccttccc gcccatcac acacacaac 
      caatcatag gttcgatta taagatttt cctgaatta 
      attgaaacg gtgccctcc atcgtggag caaatctta 
      gaattcaaa attggcgaa acgtccaat gggtggttc 
      accaatttt gactaggat ttggccaca atgtaatgt 
      aatccattt accaaagaa cgcctgcat attttattt 
      ttgctatat tttgttgca tagacttga tacaataag 
      aataaagaa agcattaat tgttgtgga aaaccgatt 
      tacggggga ctcagatat taaggggcc taactaaaa 
      atctgagaa acaaatcaa aacttatgg tatatttag 
      tctgttttg ctcgattta gttcaaaaa taagccaaa 
      aaattacca aatttaaac aagacagtg taattttta 
      cctctttac caaaaaatc acgaaatta aattatatt 
      ttataaaat tttgtttaa acaagtcaa aatcgaccg 
      aaaatggtg ttatttttg acagttttg ggcgctcct 
      tcagcgaga tatttttaa agtagagcg aaagtttag 
      gaaagtaag tcaaaattt tcaatattt tttcctttt 
      tcaaccatt ttgggacgc gattatttt gaggaattt 
      tgtttaaga taagctgag tcaaagtgc tagtaacac 
      gttttttgt ccaaaagcg cataatttt gtttaaaat 
      tacattaaa aatgattaa attgggtca caaaaagtg 
      ttattttta cctttttgc tctttttaa cctaaaagc 
      tctatacct gtaaatgct tcgaagaat caaaataca 
      aaaatgtca ttattttgc ctttcggcg ttttagtat 
      ttgaaaata tttttcgtc caaagcccg aaattgccg 
      aattgggcc gaaaaagtc gttttacga gcgaaattt 
      acagatttg ttgaattgt attaaaagt gaaatttta 
      acctttttc tagttctta aacacttat tttaaataa 
      aaaacttca agaagtttc attaaatcg gaaaaaatc 
      agtaaatga ggtcaaaat tgtcatttt tctcgaatt 
      cttagaaca gaaaggtaa tcattttta tattgaatt 
      gtattaaat ttctttctt tttaatatt tattgtaag 
      ggtcccttt tccgcactg atcatctca gacaatatg 
      catattttt tcatat
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