General information | |||
TC010119 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 14428740..14446266 | |
Sequence information | |||
Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC010119 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02874 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01651 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC010119 RA
MATLPPRRN PTPTKISKQ HLTPLQILL QMGFPKHRA EKALAATGN RGVQLASDW LLAHVNDPL LDDNSPREY ILYACPTGP FLEQLQTFW EKSHRECAW NGAHNFTPH ITLVSFFKA PDEDALHLA QSLKSVMER QGAMLNEPL KLETYTSPN FMGFFVAEE HADYLKRIA MQYVKEVSN AIISDTYEH FDALTACFP WCTTTTARC IPRGSRSIS LEPHVKSLH LTLAYQFPS THYTHLKSL VDELDPSDS AGWELRLYS RDPRVNGKQ VHKVIHPYQ PTECDELDL RTGDYVYIS SEALANSPD GWVEGTSWL TGLTGLLPE SYTERTAES DAWTLHKKV PLNHITQPE IKYTLPQKA LEMEEDASK PSAKDGSES KNGVEDYCF PTSPRIENL YENVFKTGP QKEQDKKSR KLYLMRHGE RIDFTFGVW IPYCFDESG RYTRKDLNM PSTVPERSH GPAGYTKDT PLTNVGLFQ ATTVGDALK EAQLDIAYA YSSPSFRCV QTCDALLKG YNKRDEIKI KIEPGLFEW MAWYPDGLP DWMTPAELA AAGYNIDLD YQPFVTVTE LKDARETCE QYYLRNTFV TRGALNAHL DGNVLFVGH ASTLEVCSR ELVGKKPRA AYEMTDVIQ KVAYCSLLQ LAQEGDKWE IAELPFPPI THTTNHRFD YKIFLN
- >TC010119 RB
MATLPPRRN PTPTKISKQ HLTPLQILL QMGFPKHRA EKALAATGN RGVQLASDW LLAHVNDPL LDDNSPREY ILYACPTGP FLEQLQTFW EKSHRECAW NGAHNFTPH ITLVSFFKA PDEDALHLA QSLKSVMER QGAMLNEPL KLETYTSPN FMGFFVAEE HADYLKRIA MQYVKEVSN ATISLEPHV KSLHLTLAY QFPSTHYTH LKSLVDELD PSDSAGWEL RLYSRDPRV NGKQVHKVI HPYQPTECD ELDLRTGDY VYISSEALA NSPDGWVEG TSWLTGLTG LLPESYTER TAESDAWTL HKKVPLNHI TQPEIKYTL PQKALEMEE DASKPSAKD GSESKNGVE DYCFPTSPR IENLYENVF KTGPQKEQD KKSRKLYLM RHGERIDFT FGVWIPYCF DESGRYTRK DLNMPSTVP ERSHGPAGY TKDTPLTNV GLFQATTVG DALKEAQLD IAYAYSSPS FRCVQTCDA LLKGYNKRD EIKIKIEPG LFEWMAWYP DGLPDWMTP AELAAAGYN IDLDYQPFV TVTELKDAR ETCEQYYLR NTFVTRGAL NAHLDGNVL FVGHASTLE VCSRELVGK KPRAAYEMT DVIQKVAYC SLLQLAQEG DKWEIAELP FPPITHTTN HRFDYKIFL N
- >TC010119 RA
atggcaaca ctaccccct aggaggaac cccacacct acaaaaata tccaaacag cacctgacc ccgctacaa atcctcctc cagatgggg ttccccaaa cacagagcc gaaaaggcc ttagctgcc actggtaac aggggcgtg caattggcc tccgactgg ttactggcc cacgtaaat gacccattg ctggacgat aactctcca agggagtac attttatat gcgtgtccc acggggcct ttcctggaa cagttacag accttctgg gagaagtcg cacagggaa tgcgcctgg aacggggcc cacaatttc acgccgcac attacgcta gtttcgttt tttaaggcg cctgatgaa gacgcccta catctggcg caaagccta aagtccgta atggaaagg cagggcgct atgttgaac gagccccta aaactagaa acgtatact tctcccaat tttatgggg ttttttgtg gcggaggag cacgctgat tatttaaaa aggattgcc atgcaatat gttaaagaa gtttcaaat gctattatt agtgacaca tacgaacat ttcgatgct ctaacagcc tgttttccg tggtgtacc acaacaact gctcgttgc attcctcgg ggaagtaga tctataagt cttgaaccc cacgtaaaa tcgctccat ttgacgttg gcgtaccaa ttccccagc acccactac acccacctc aaatccctc gtggacgag ctcgaccct agcgactcc gccggctgg gagttgcgt ctctactcc cgcgatccc cgcgtaaac ggtaaacaa gtccacaaa gtgattcac ccctaccaa ccaaccgag tgtgacgaa ttggatctt cgcacgggc gattacgtc tacataagc agcgaagcc ttggccaat tcgcccgac ggttgggtc gagggcact tcctggttg acgggactg acgggactc ttgccagag agctatacg gaacggacg gcggagtcc gatgcgtgg actctgcac aaaaaagtg ccactgaat catatcact caaccggaa attaagtac accttaccc caaaaagct ttagagatg gaggaggac gcctccaag ccaagtgcc aaggatggg agcgaatcg aagaatggg gttgaagat tactgcttt cccacgtcg ccgaggatc gagaatttg tacgaaaat gtgttcaaa acggggcct caaaaggag caagacaaa aaatcccgc aaactctac ctgatgcgt cacggggag cgcatagac ttcaccttc ggcgtctgg atcccctat tgctttgac gagtctggc cgctacacc cggaaagac ctgaatatg ccatccaca gtacctgag cgatcgcac ggccccgcc ggctacacc aaggacacc cccttgacc aacgtcggt ctcttccag gccaccacc gtcggggac gccctcaaa gaagcgcaa ctcgacatc gcatacgcc tactcctcg ccctcgttc cggtgcgtc cagacctgc gacgccctc ctcaaaggc tacaacaaa cgcgacgaa atcaaaatc aaaatcgag cctggactt ttcgaatgg atggcctgg tatccggac gggctgccc gactggatg acaccggcc gaactggcc gcagctggc tacaacata gacctggac tatcagccg ttcgtgacc gtaacggag ctgaaagac gccagggag acttgcgag cagtattat ttgcgcaac actttcgtc acgagaggg gctctcaat gcgcacctc gacggaaac gtgctgttt gtgggccac gcgtccact ttggaagtg tgttcgagg gaattggtc gggaagaag ccgcgggct gcgtatgaa atgaccgat gttatacag aaagtggcc tattgtagt ttactgcaa ctggcgcaa gagggcgac aagtgggag attgccgag ctgcccttc ccgcccatc acacacaca accaatcat aggttcgat tataagatt ttcctgaat taa
- >TC010119 RB
atggcaaca ctaccccct aggaggaac cccacacct acaaaaata tccaaacag cacctgacc ccgctacaa atcctcctc cagatgggg ttccccaaa cacagagcc gaaaaggcc ttagctgcc actggtaac aggggcgtg caattggcc tccgactgg ttactggcc cacgtaaat gacccattg ctggacgat aactctcca agggagtac attttatat gcgtgtccc acggggcct ttcctggaa cagttacag accttctgg gagaagtcg cacagggaa tgcgcctgg aacggggcc cacaatttc acgccgcac attacgcta gtttcgttt tttaaggcg cctgatgaa gacgcccta catctggcg caaagccta aagtccgta atggaaagg cagggcgct atgttgaac gagccccta aaactagaa acgtatact tctcccaat tttatgggg ttttttgtg gcggaggag cacgctgat tatttaaaa aggattgcc atgcaatat gttaaagaa gtttcaaat gctactata agtcttgaa ccccacgta aaatcgctc catttgacg ttggcgtac caattcccc agcacccac tacacccac ctcaaatcc ctcgtggac gagctcgac cctagcgac tccgccggc tgggagttg cgtctctac tcccgcgat ccccgcgta aacggtaaa caagtccac aaagtgatt cacccctac caaccaacc gagtgtgac gaattggat cttcgcacg ggcgattac gtctacata agcagcgaa gccttggcc aattcgccc gacggttgg gtcgagggc acttcctgg ttgacggga ctgacggga ctcttgcca gagagctat acggaacgg acggcggag tccgatgcg tggactctg cacaaaaaa gtgccactg aatcatatc actcaaccg gaaattaag tacacctta ccccaaaaa gctttagag atggaggag gacgcctcc aagccaagt gccaaggat gggagcgaa tcgaagaat ggggttgaa gattactgc tttcccacg tcgccgagg atcgagaat ttgtacgaa aatgtgttc aaaacgggg cctcaaaag gagcaagac aaaaaatcc cgcaaactc tacctgatg cgtcacggg gagcgcata gacttcacc ttcggcgtc tggatcccc tattgcttt gacgagtct ggccgctac acccggaaa gacctgaat atgccatcc acagtacct gagcgatcg cacggcccc gccggctac accaaggac acccccttg accaacgtc ggtctcttc caggccacc accgtcggg gacgccctc aaagaagcg caactcgac atcgcatac gcctactcc tcgccctcg ttccggtgc gtccagacc tgcgacgcc ctcctcaaa ggctacaac aaacgcgac gaaatcaaa atcaaaatc gagcctgga cttttcgaa tggatggcc tggtatccg gacgggctg cccgactgg atgacaccg gccgaactg gccgcagct ggctacaac atagacctg gactatcag ccgttcgtg accgtaacg gagctgaaa gacgccagg gagacttgc gagcagtat tatttgcgc aacactttc gtcacgaga ggggctctc aatgcgcac ctcgacgga aacgtgctg tttgtgggc cacgcgtcc actttggaa gtgtgttcg agggaattg gtcgggaag aagccgcgg gctgcgtat gaaatgacc gatgttata cagaaagtg gcctattgt agtttactg caactggcg caagagggc gacaagtgg gagattgcc gagctgccc ttcccgccc atcacacac acaaccaat cataggttc gattataag attttcctg aattaa
- >TC010119 RA
cctcgatca aatttcttt ccctcagga tcaatatct ttcacgttg aatatatct tcaaacagg acacctgcc atcgtcgaa taacgtgta aataataaa aaattaaca cactttaat actttctta tgaaaatgg gcaaatttt gacagacat caacacttg acagtgttc caaccagtg ccgccatct gaaaggcca attccccaa acaaaacca aaacaaaaa cgtcactgt ttaaatgtc aaaggtcca ctagaagtg tcaacacca aactttaac ctagttgaa attaataat ttattaaat gaaaatggc aacactacc ccctaggag gaaccccac acctacaaa aatatccaa acagcacct gaccccgct acaaatcct cctccagat ggggttccc caaacacag agccgaaaa ggccttagc tgccactgg taacagggg cgtgcaatt ggcctccga ctggttact ggcccacgt aaatgaccc attgctgga cgataactc tccaaggga gtacatttt atatgcgtg tcccacggg gcctttcct ggaacagtt acagacctt ctgggagaa gtcgcacag ggaatgcgc ctggaacgg ggcccacaa tttcacgcc gcacattac gctagtttc gttttttaa ggcgcctga tgaagacgc cctacatct ggcgcaaag cctaaagtc cgtaatgga aaggcaggg cgctatgtt gaacgagcc cctaaaact agaaacgta tacttctcc caattttat ggggttttt tgtggcgga ggagcacgc tgattattt aaaaaggat tgccatgca atatgttaa agaagtttc aaatgctat tattagtga cacatacga acatttcga tgctctaac agcctgttt tccgtggtg taccacaac aactgctcg ttgcattcc tcggggaag tagatctat aagtcttga accccacgt aaaatcgct ccatttgac gttggcgta ccaattccc cagcaccca ctacaccca cctcaaatc cctcgtgga cgagctcga ccctagcga ctccgccgg ctgggagtt gcgtctcta ctcccgcga tccccgcgt aaacggtaa acaagtcca caaagtgat tcaccccta ccaaccaac cgagtgtga cgaattgga tcttcgcac gggcgatta cgtctacat aagcagcga agccttggc caattcgcc cgacggttg ggtcgaggg cacttcctg gttgacggg actgacggg actcttgcc agagagcta tacggaacg gacggcgga gtccgatgc gtggactct gcacaaaaa agtgccact gaatcatat cactcaacc ggaaattaa gtacacctt accccaaaa agctttaga gatggagga ggacgcctc caagccaag tgccaagga tgggagcga atcgaagaa tggggttga agattactg ctttcccac gtcgccgag gatcgagaa tttgtacga aaatgtgtt caaaacggg gcctcaaaa ggagcaaga caaaaaatc ccgcaaact ctacctgat gcgtcacgg ggagcgcat agacttcac cttcggcgt ctggatccc ctattgctt tgacgagtc tggccgcta cacccggaa agacctgaa tatgccatc cacagtacc tgagcgatc gcacggccc cgccggcta caccaagga caccccctt gaccaacgt cggtctctt ccaggccac caccgtcgg ggacgccct caaagaagc gcaactcga catcgcata cgcctactc ctcgccctc gttccggtg cgtccagac ctgcgacgc cctcctcaa aggctacaa caaacgcga cgaaatcaa aatcaaaat cgagcctgg acttttcga atggatggc ctggtatcc ggacgggct gcccgactg gatgacacc ggccgaact ggccgcagc tggctacaa catagacct ggactatca gccgttcgt gaccgtaac ggagctgaa agacgccag ggagacttg cgagcagta ttatttgcg caacacttt cgtcacgag aggggctct caatgcgca cctcgacgg aaacgtgct gtttgtggg ccacgcgtc cactttgga agtgtgttc gagggaatt ggtcgggaa gaagccgcg ggctgcgta tgaaatgac cgatgttat acagaaagt ggcctattg tagtttact gcaactggc gcaagaggg cgacaagtg ggagattgc cgagctgcc cttcccgcc catcacaca cacaaccaa tcataggtt cgattataa gattttcct gaattaatt gaaacggtg ccctccatc gtggagcaa atcttagaa ttcaaaatt ggcgaaacg tccaatggg tggttcacc aattttgac taggatttg gccacaatg taatgtaat ccatttacc aaagaacgc ctgcatatt ttatttttg ctatatttt gttgcatag acttgatac aataagaat aaagaaagc attaattgt tgtggaaaa ccgatttac gggggactc agatattaa ggggcctaa ctaaaaatc tgagaaaca aatcaaaac ttatggtat atttagtct gttttgctc gatttagtt caaaaataa gccaaaaaa ttaccaaat ttaaacaag acagtgtaa tttttacct ctttaccaa aaaatcacg aaattaaat tatatttta taaaatttt gtttaaaca agtcaaaat cgaccgaaa atggtgtta tttttgaca gttttgggc gctccttca gcgagatat ttttaaagt agagcgaaa gtttaggaa agtaagtca aaattttca atatttttt cctttttca accattttg ggacgcgat tattttgag gaattttgt ttaagataa gctgagtca aagtgctag taacacgtt ttttgtcca aaagcgcat aattttgtt taaaattac attaaaaat gattaaatt gggtcacaa aaagtgtta tttttacct ttttgctct ttttaacct aaaagctct atacctgta aatgcttcg aagaatcaa aatacaaaa atgtcatta ttttgcctt tcggcgttt tagtatttg aaaatattt ttcgtccaa agcccgaaa ttgccgaat tgggccgaa aaagtcgtt ttacgagcg aaatttaca gatttgttg aattgtatt aaaagtgaa attttaacc tttttctag ttcttaaac acttatttt aaataaaaa acttcaaga agtttcatt aaatcggaa aaaatcagt aaatgaggt caaaattgt catttttct cgaattctt agaacagaa aggtaatca tttttatat tgaattgta ttaaatttc tttcttttt aatatttat tgtaagggt cccttttcc gcactgatc atctcagac aatatgcat attttttca tat
- >TC010119 RB
cctcgatca aatttcttt ccctcagga tcaatatct ttcacgttg aatatatct tcaaacagg acacctgcc atcgtcgaa taacgtgta aataataaa aaattaaca cactttaat actttctta tgaaaatgg gcaaatttt gacagacat caacacttg acagtgttc caaccagtg ccgccatct gaaaggcca attccccaa acaaaacca aaacaaaaa cgtcactgt ttaaatgtc aaaggtcca ctagaagtg tcaacacca aactttaac ctagttgaa attaataat ttattaaat gaaaatggc aacactacc ccctaggag gaaccccac acctacaaa aatatccaa acagcacct gaccccgct acaaatcct cctccagat ggggttccc caaacacag agccgaaaa ggccttagc tgccactgg taacagggg cgtgcaatt ggcctccga ctggttact ggcccacgt aaatgaccc attgctgga cgataactc tccaaggga gtacatttt atatgcgtg tcccacggg gcctttcct ggaacagtt acagacctt ctgggagaa gtcgcacag ggaatgcgc ctggaacgg ggcccacaa tttcacgcc gcacattac gctagtttc gttttttaa ggcgcctga tgaagacgc cctacatct ggcgcaaag cctaaagtc cgtaatgga aaggcaggg cgctatgtt gaacgagcc cctaaaact agaaacgta tacttctcc caattttat ggggttttt tgtggcgga ggagcacgc tgattattt aaaaaggat tgccatgca atatgttaa agaagtttc aaatgctac tataagtct tgaacccca cgtaaaatc gctccattt gacgttggc gtaccaatt ccccagcac ccactacac ccacctcaa atccctcgt ggacgagct cgaccctag cgactccgc cggctggga gttgcgtct ctactcccg cgatccccg cgtaaacgg taaacaagt ccacaaagt gattcaccc ctaccaacc aaccgagtg tgacgaatt ggatcttcg cacgggcga ttacgtcta cataagcag cgaagcctt ggccaattc gcccgacgg ttgggtcga gggcacttc ctggttgac gggactgac gggactctt gccagagag ctatacgga acggacggc ggagtccga tgcgtggac tctgcacaa aaaagtgcc actgaatca tatcactca accggaaat taagtacac cttacccca aaaagcttt agagatgga ggaggacgc ctccaagcc aagtgccaa ggatgggag cgaatcgaa gaatggggt tgaagatta ctgctttcc cacgtcgcc gaggatcga gaatttgta cgaaaatgt gttcaaaac ggggcctca aaaggagca agacaaaaa atcccgcaa actctacct gatgcgtca cggggagcg catagactt caccttcgg cgtctggat cccctattg ctttgacga gtctggccg ctacacccg gaaagacct gaatatgcc atccacagt acctgagcg atcgcacgg ccccgccgg ctacaccaa ggacacccc cttgaccaa cgtcggtct cttccaggc caccaccgt cggggacgc cctcaaaga agcgcaact cgacatcgc atacgccta ctcctcgcc ctcgttccg gtgcgtcca gacctgcga cgccctcct caaaggcta caacaaacg cgacgaaat caaaatcaa aatcgagcc tggactttt cgaatggat ggcctggta tccggacgg gctgcccga ctggatgac accggccga actggccgc agctggcta caacataga cctggacta tcagccgtt cgtgaccgt aacggagct gaaagacgc cagggagac ttgcgagca gtattattt gcgcaacac tttcgtcac gagaggggc tctcaatgc gcacctcga cggaaacgt gctgtttgt gggccacgc gtccacttt ggaagtgtg ttcgaggga attggtcgg gaagaagcc gcgggctgc gtatgaaat gaccgatgt tatacagaa agtggccta ttgtagttt actgcaact ggcgcaaga gggcgacaa gtgggagat tgccgagct gcccttccc gcccatcac acacacaac caatcatag gttcgatta taagatttt cctgaatta attgaaacg gtgccctcc atcgtggag caaatctta gaattcaaa attggcgaa acgtccaat gggtggttc accaatttt gactaggat ttggccaca atgtaatgt aatccattt accaaagaa cgcctgcat attttattt ttgctatat tttgttgca tagacttga tacaataag aataaagaa agcattaat tgttgtgga aaaccgatt tacggggga ctcagatat taaggggcc taactaaaa atctgagaa acaaatcaa aacttatgg tatatttag tctgttttg ctcgattta gttcaaaaa taagccaaa aaattacca aatttaaac aagacagtg taattttta cctctttac caaaaaatc acgaaatta aattatatt ttataaaat tttgtttaa acaagtcaa aatcgaccg aaaatggtg ttatttttg acagttttg ggcgctcct tcagcgaga tatttttaa agtagagcg aaagtttag gaaagtaag tcaaaattt tcaatattt tttcctttt tcaaccatt ttgggacgc gattatttt gaggaattt tgtttaaga taagctgag tcaaagtgc tagtaacac gttttttgt ccaaaagcg cataatttt gtttaaaat tacattaaa aatgattaa attgggtca caaaaagtg ttattttta cctttttgc tctttttaa cctaaaagc tctatacct gtaaatgct tcgaagaat caaaataca aaaatgtca ttattttgc ctttcggcg ttttagtat ttgaaaata tttttcgtc caaagcccg aaattgccg aattgggcc gaaaaagtc gttttacga gcgaaattt acagatttg ttgaattgt attaaaagt gaaatttta acctttttc tagttctta aacacttat tttaaataa aaaacttca agaagtttc attaaatcg gaaaaaatc agtaaatga ggtcaaaat tgtcatttt tctcgaatt cttagaaca gaaaggtaa tcattttta tattgaatt gtattaaat ttctttctt tttaatatt tattgtaag ggtcccttt tccgcactg atcatctca gacaatatg catattttt tcatat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
