Status
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Gene information
General information
TC033827
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008762
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9617307..9620932
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033827
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02871

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033827 RA
      MGLFSKFAF FLGVRKKEV NVLVVGLNN 
      SGKSTVVNH FKNEEERVT EIVPTVGFS 
      VEKFQNQNL AFTAFDMSG HGRYRDLWE 
      HYYKDCHGI IFVVDSSDR LRLVVVKEE 
      LDLLLQHPD ICNRKIPVL FFANKMDCK 
      DALSSVKIA AGLGLDKIM DKPWHIAAS 
      NALTGEGLQ EGVEWLTQQ IRDVVLNKS
    CDS sequence
    • >TC033827 RA
      atgggtttg tttagtaaa tttgccttt ttccttggt 
      gtgcgaaaa aaagaagtt aatgttttg gtagttgga 
      cttaataat agtggtaaa tcgacagtt gtcaaccat 
      ttcaaaaat gaagaagaa cgcgttact gagatcgta 
      ccaacagtg gggttcagc gtcgaaaaa tttcaaaat 
      caaaatttg gctttcaca gcctttgat atgtctggg 
      catggacgc taccgcgac ctgtgggaa cattattac 
      aaggactgc cacgggata atttttgtg gtggacagc 
      agtgaccgc cttcgactg gttgtcgtt aaagaagaa 
      ttggattta ttgttgcaa cacccagac atatgtaat 
      cgcaaaatt ccagttctc ttttttgcg aacaaaatg 
      gactgcaaa gacgcttta agcagtgtt aaaattgcc 
      gcaggactg gggttggat aaaataatg gataagccg 
      tggcatatc gctgcaagt aacgctctg actggggaa 
      ggcctgcag gaaggagtt gaatggttg acacagcaa 
      atcagagat gttgtcttg aataagagt taa
    mRNA sequence
    • >TC033827 RA
      actgttgta gaaaagctc atttcaaac tgttcttca 
      ccttatttt gatggtagc agtcccatt cttcgtggt 
      aatggtcgc gctttactt agcgtttgc ctttggggc 
      ctttgggcc tgcctcgga aagatgtga cgctctatg 
      ctgttattt agcaaataa aactctaaa tatttacga 
      caaacgcga tcgcgaata catagaata tgaatttga 
      acttgatgc ccttaacaa aagatctat tagttctct 
      tagccaaac cttcactct tgcaaagtg taaggaatc 
      agagaatcc agcaattgc cgctcttct cggaaagcg 
      atatattga tgcaaaaga aggattttt caaatgtgc 
      tgttggtgt tattgcgcg aaactgggc cctagctgt 
      tgatgactg tgttgaaaa tttgggcgt tctgagatt 
      agggtcgtc atggtggtc tttcggggg agtgacaaa 
      aagggtcaa taatgtcat tttttctaa ttttggttt 
      ggttttgct acattttaa tgcaactga cgataaggc 
      atttgaaat tgcgaaact tgatcgatt tgtacagag 
      ctttacttg ggataggag agtggatct cccgttggc 
      acaaaggca aaaattcaa caaattgct cagttttga 
      tgttcgtgt ttatcaaca cgaggactg tacgggttt 
      catcaaaca gaagcatgt ttcttgtta catttcaag 
      caggaacga gttttgtgg aaaaagggt taatgctcc 
      aatgatcga ccaaagtaa caacttggt gggaatgca 
      tttggagac tttttgccc aaaaaatga aagttatgc 
      gattgtttt ttacattga tgtttattc ttacaaaac 
      ctggatttt ggacatggt taataaatt ttgaacaca 
      caaatttcg tttttttta atcacctag tgaccgttt 
      cttgagtga ccacaagtg cgtaccaag tgacaagca 
      agtcaaatt tcgtagaat gtttttgat accacaaga 
      cccaaaagg tgttcggcc caaagtaaa gcaacttaa 
      aagatctta atttgtcat tagcaagtt ttatagaca 
      tgttggacg aaaagatct gcctttcaa acgggtctc 
      attttgata ctttttgtg gcagttcca tttttcata 
      gtaatggtt gctctgtac gggcattct ctaacggcc 
      tggaagcaa acagaaaaa tgctttcgg ttgtgcact 
      ttgcttcgc aattggaaa atgaaacga cagcttaca 
      gatcccacg ggtaaaaga aaaatttct aatgtttat 
      ttccaaagt tttctaatt tcataatag attatccca 
      gttttcttg aagagatag catcgaaag acatagaag 
      acatggaaa gacgcaaac gggatgctg cgactttga 
      atcaacgcg gcaacgaca atgagatgc tcagtatga 
      aactggagc aaaaaagaa aagaaaccg aagaaaaat 
      atgaagaag aaggcttga gtattcaac attactttc 
      ctgtggcag ctttactag aaaagatgt actttgacg 
      gaaatgaag atcgattta aatgcacaa ttgcaaaac 
      tttcaacca actgggcac tgacaacga ctgattgtt 
      ttaattata atagcgaca attgggact tgttatcag 
      ttcgcagca actcgcatt actggtcta aaggtggct 
      taataaaaa aaatagaaa atgtttttt ggtacgaga 
      agctcgtgg caatcaaat taaaatctg atcaagctt 
      ctacacgat tttacatct cccagtttg taaatgttt 
      caaatagag aacaaatat tctattggg acgttaatc 
      tacatcttc cattacaaa aaagagtga aatttggtg 
      aactccaaa ttacaaata tgcccctag agaaagcac 
      tgcatgtgc ccatatttg attacggca aacatttga 
      ttggtttcc agtcgttta aattcactt acaaaaatg 
      taaccgcct atcgttctt ggttaaggg aactgggag 
      ctttcccat aaatgtgtt tctcctgct gttcatcga 
      ttgtttcaa accatggta acgctgggc tataataca 
      aacacacgt catgcgtta tgggtttgt ttagtaaat 
      ttgcctttt tccttggtg tgcgaaaaa aagaagtta 
      atgttttgg tagttggac ttaataata gtggtaaat 
      cgacagttg tcaaccatt tcaaaaatg aagaagaac 
      gcgttactg agatcgtac caacagtgg ggttcagcg 
      tcgaaaaat ttcaaaatc aaaatttgg ctttcacag 
      cctttgata tgtctgggc atggacgct accgcgacc 
      tgtgggaac attattaca aggactgcc acgggataa 
      tttttgtgg tggacagca gtgaccgcc ttcgactgg 
      ttgtcgtta aagaagaat tggatttat tgttgcaac 
      acccagaca tatgtaatc gcaaaattc cagttctct 
      tttttgcga acaaaatgg actgcaaag acgctttaa 
      gcagtgtta aaattgccg caggactgg ggttggata 
      aaataatgg ataagccgt ggcatatcg ctgcaagta 
      acgctctga ctggggaag gcctgcagg aaggagttg 
      aatggttga cacagcaaa tcagagatg ttgtcttga 
      ataagagtt aatgttatt tgtttgcat attaaaact 
      tttatatac tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY