Status
Please wait ...
Gene information
General information
TC033818
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008782
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9842563..9845054
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033818
    iBeetle screen iB_02869

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033818 RA
      MPKHDYSSH RSRSPVRSR KSTNKKYSS DSSDGYKRH 
      DSKKRSHSR KDKKSGHRS DSSRKDHKK HKRRDSESD 
      SSSSSTSSD DSLVLLQKL KEERIKALE ERKREKELR 
      KATETPEEK RLRRLAKKQ AKERKRKER MGWDNEYLH 
      YTNTDNPFG DGNLLSTFV WTKKLAKEG LTGVTPEEL 
      EARNRHKQE ENKRELEKV KKRRLEREL ERQKREEEM 
      QLLQRSKEA AQFEEWERQ EDQFHLEQA RLRSHIRIQ 
      DGRAKPIDL LAKYISAEE EVDAVEMHE PYTYLNGLH 
      IKDLEDLVE DIKVYEELE RGKNLDYWN DITVIVEDE 
      LQKLRKLEK QNEFDMGVN RREGINQAV AADVTSVFK 
      GKTSSQLAA LQKQIESKI NGKADGIDI GYWESLLSQ 
      LKAHMARAR LRDRHQDSL RRKLQVLKA EQAVTTPAP 
      EETTPSTES HVKEEPEPK PGTSQEEKS DDENEKTAD 
      EHASDILNE YFDAYEAGG YSPKFLSPE DIEPGTIVV 
      TEEDDLKRL EFARMQMSG GGKKHEHAI TKEEILLQR 
      EARKGMGDD EAQFSVEAA LDNQVYLWS DKYRPRKPR 
      YFNRVHTGF EWNKYNQTH YDMDNPPPK IVQGYKFNI 
      FYPDLIDKN STPEYFLTP CPENREFAI LRFHAGPPY 
      EDIAFKIVN REWEYSYKR GFRCQFHNN IFQLWFHFK 
      RYRYRR
    CDS sequence
    • >TC033818 RA
      atgccaaaa catgactac tccagtcat cgctccagg 
      tcaccagtt cgctcccgc aaatcaaca aacaaaaaa 
      tactcgtca gattcaagt gacggttac aaaaggcac 
      gattcaaag aaacgttcg cattccaga aaagataag 
      aaaagtggc catcggagt gactcttct agaaaagat 
      cataaaaaa cataaacga cgcgactca gagtcagac 
      tcaagcagt agcagcacc tcctctgat gactcttta 
      gtattgctc caaaaattg aaagaagaa cgaattaaa 
      gctcttgaa gagcgcaaa cgtgagaaa gaactaagg 
      aaggccact gaaacgcca gaggagaag cgtttgaga 
      cgtttagcc aaaaaacaa gcgaaagag aggaaacgc 
      aaagaacgt atggggtgg gacaatgaa tatctccat 
      tataccaat acagataac ccgtttggt gacgggaat 
      ctactatca acgtttgtg tggacaaaa aaattggcg 
      aaagagggg ctaactggt gtgactcct gaagaacta 
      gaagcgagg aatcgccac aaacaggag gagaacaaa 
      cgagaattg gaaaaggta aagaagcga agattagag 
      agggaacta gagagacag aagcgggag gaagaaatg 
      cagttacta caaaggagt aaagaggcg gcgcagttt 
      gaagaatgg gaacgtcaa gaggaccag tttcattta 
      gaacaagcc agactaaga tcacacatt cgaatacaa 
      gacgggcga gctaaaccg attgattta ctcgcaaaa 
      tatatcagt gcagaggaa gaagtagac gccgttgag 
      atgcacgaa ccttacact tacctaaac gggttacac 
      atcaaagat ttagaagac ttggttgaa gacattaaa 
      gtttatgag gagctagaa cgggggaaa aacctagac 
      tattggaac gacattacc gtaatcgtc gaagacgag 
      ctgcaaaaa ctccgaaaa ttagaaaag caaaatgaa 
      ttcgacatg ggggtgaac agaagagaa ggcatcaat 
      caggcagtt gccgccgac gtcacctca gtcttcaaa 
      ggaaagact tcgtcccag ctggcggcc ctgcagaaa 
      caaatcgaa agcaagata aacggcaaa gctgatggc 
      atagatata ggctactgg gagtcgcta ctgtcgcaa 
      ctaaaagct cacatggcc agagcccgc ttgcgagac 
      cgccaccag gacagtctc cgacgcaaa ctgcaagtc 
      cttaaagcc gaacaagcg gtcacaacc ccagcccct 
      gaggaaaca accccatct acggaatcc cacgtcaag 
      gaagaacct gaacccaaa cccgggact tcccaggag 
      gagaaaagt gacgatgaa aatgaaaag accgcggac 
      gaacacgcc agcgacatc ctgaacgaa tacttcgac 
      gcgtacgaa gccggcggc tacagcccc aagttcctc 
      tcacccgaa gacattgag cccgggact atcgtcgtg 
      accgaagaa gatgacctc aaacgcctg gaattcgca 
      cgcatgcag atgtctgga ggcggcaaa aagcacgaa 
      cacgccata actaaggag gaaatttta ctacaacgc 
      gaagcccgc aagggcatg ggtgacgat gaggcccag 
      ttttcggtc gaagcggct ctggacaac caagtgtac 
      ttgtggtcc gataagtac agaccgagg aaacccaga 
      tacttcaat agggtgcac acaggtttt gagtggaat 
      aagtacaac cagacccat tatgacatg gacaatccg 
      cccccgaaa atcgtacag ggctacaag tttaacatt 
      ttttacccg gatttgatc gacaagaat agcacgccc 
      gagtatttt ttgactccg tgtccagaa aatcgggaa 
      tttgccatt ttgcggttc cacgcaggg ccgccgtat 
      gaagacatt gcgtttaag atcgtgaac agggagtgg 
      gaatattcg tacaagagg gggttcagg tgccagttt 
      cacaacaac atttttcaa ttgtggttc cactttaag 
      aggtacaga taccgaaga tag
    mRNA sequence
    • >TC033818 RA
      agaaaaaca ggataaagc aggtaagtt ggatttctg 
      cccaaaggt tggttgcga tggaagaat tctcgtgag 
      cgtacattc tcgtctgcc atacacgac gtatgatct 
      acgattgac agtagtttg acactcact aaccttaaa 
      atcaaaaca tttgttcag aaataaaat gccaaaaca 
      tgactactc cagtcatcg ctccaggtc accagttcg 
      ctcccgcaa atcaacaaa caaaaaata ctcgtcaga 
      ttcaagtga cggttacaa aaggcacga ttcaaagaa 
      acgttcgca ttccagaaa agataagaa aagtggcca 
      tcggagtga ctcttctag aaaagatca taaaaaaca 
      taaacgacg cgactcaga gtcagactc aagcagtag 
      cagcacctc ctctgatga ctctttagt attgctcca 
      aaaattgaa agaagaacg aattaaagc tcttgaaga 
      gcgcaaacg tgagaaaga actaaggaa ggccactga 
      aacgccaga ggagaagcg tttgagacg tttagccaa 
      aaaacaagc gaaagagag gaaacgcaa agaacgtat 
      ggggtggga caatgaata tctccatta taccaatac 
      agataaccc gtttggtga cgggaatct actatcaac 
      gtttgtgtg gacaaaaaa attggcgaa agaggggct 
      aactggtgt gactcctga agaactaga agcgaggaa 
      tcgccacaa acaggagga gaacaaacg agaattgga 
      aaaggtaaa gaagcgaag attagagag ggaactaga 
      gagacagaa gcgggagga agaaatgca gttactaca 
      aaggagtaa agaggcggc gcagtttga agaatggga 
      acgtcaaga ggaccagtt tcatttaga acaagccag 
      actaagatc acacattcg aatacaaga cgggcgagc 
      taaaccgat tgatttact cgcaaaata tatcagtgc 
      agaggaaga agtagacgc cgttgagat gcacgaacc 
      ttacactta cctaaacgg gttacacat caaagattt 
      agaagactt ggttgaaga cattaaagt ttatgagga 
      gctagaacg ggggaaaaa cctagacta ttggaacga 
      cattaccgt aatcgtcga agacgagct gcaaaaact 
      ccgaaaatt agaaaagca aaatgaatt cgacatggg 
      ggtgaacag aagagaagg catcaatca ggcagttgc 
      cgccgacgt cacctcagt cttcaaagg aaagacttc 
      gtcccagct ggcggccct gcagaaaca aatcgaaag 
      caagataaa cggcaaagc tgatggcat agatatagg 
      ctactggga gtcgctact gtcgcaact aaaagctca 
      catggccag agcccgctt gcgagaccg ccaccagga 
      cagtctccg acgcaaact gcaagtcct taaagccga 
      acaagcggt cacaacccc agcccctga ggaaacaac 
      cccatctac ggaatccca cgtcaagga agaacctga 
      acccaaacc cgggacttc ccaggagga gaaaagtga 
      cgatgaaaa tgaaaagac cgcggacga acacgccag 
      cgacatcct gaacgaata cttcgacgc gtacgaagc 
      cggcggcta cagccccaa gttcctctc acccgaaga 
      cattgagcc cgggactat cgtcgtgac cgaagaaga 
      tgacctcaa acgcctgga attcgcacg catgcagat 
      gtctggagg cggcaaaaa gcacgaaca cgccataac 
      taaggagga aattttact acaacgcga agcccgcaa 
      gggcatggg tgacgatga ggcccagtt ttcggtcga 
      agcggctct ggacaacca agtgtactt gtggtccga 
      taagtacag accgaggaa acccagata cttcaatag 
      ggtgcacac aggttttga gtggaataa gtacaacca 
      gacccatta tgacatgga caatccgcc cccgaaaat 
      cgtacaggg ctacaagtt taacatttt ttacccgga 
      tttgatcga caagaatag cacgcccga gtatttttt 
      gactccgtg tccagaaaa tcgggaatt tgccatttt 
      gcggttcca cgcagggcc gccgtatga agacattgc 
      gtttaagat cgtgaacag ggagtggga atattcgta 
      caagagggg gttcaggtg ccagtttca caacaacat 
      ttttcaatt gtggttcca ctttaagag gtacagata 
      ccgaagata gctcagttt tgctgtaat gatatctgt 
      cttaatgta tataataaa gttttatga aaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY