Status
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Gene information
General information
TC033798
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008813,TC008814
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10235531..10333324
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033798
    iBeetle screen iB_06259

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_02867

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033798 RA
      MRWVLVFGA ICCCVTCQT LVPDEEEAA IVSSVDAVL 
      GRSATLPCD IDPGIREDR VYMVLWYRD THTKPIYSF 
      DVRGRPFNK AIHWSDPMV FGPRAYFVT VSKPAALTL 
      DGVTLNDEG IYRCRVDFR TTPTRNFAI NLTVIVPPH 
      QVLVYDNSG RDIVGIVGP LEEGADLVL TCEVRGGRP 
      TPTVSWFMN ERLVEGDVE AIGDTLMVN RLAVKAVRR 
      EHLNSTYKC QASNTKLMM PAEKTVRLE LLLRPLSVV 
      IPYKPRQMV AKQDYTIQC QVTGSRPKA TVTWTRDNR 
      DFRRGKFLE EGNETVVIS SVTFSPEPE DDGTFLKCL 
      GDNPKLPGF SQEDSFKLN VVSCGEIFT SNGYSHGPD 
      VRQHFKQTS VEKETDRRL FADVYWENR NE
    CDS sequence
    • >TC033798 RA
      atgcgttgg gttctcgtt tttggggcg atttgctgc 
      tgtgtgact tgtcagacg ttggtgccc gacgaggag 
      gaagcagcg atcgtctca tcggtggac gcagttctg 
      ggtcggagc gccacacta ccatgtgac atcgaccca 
      ggcatcagg gaagaccgg gtgtacatg gtgctgtgg 
      tacagagac acccacacc aagcccatc tacagcttc 
      gacgtccgc ggccgccct ttcaacaaa gcgatccac 
      tggtcggac cccatggtg ttcggcccc cgagcttac 
      ttcgtgacg gtctcaaag cccgccgcc ctcaccctg 
      gacggggtg actctgaac gacgaaggc atctaccga 
      tgtcgggtg gatttccgc acaacgccg accaggaat 
      ttcgccatc aatcttacg gtcattgtt cctccgcac 
      caggtgctg gtgtacgat aactccgga cgggacatc 
      gttgggatc gtgggtcct ctggaggag ggtgcggac 
      ctggtgcta acttgcgag gttcgagga gggcggccg 
      acgccgacg gtctcctgg ttcatgaac gagcggctg 
      gtcgagggg gacgtggag gccatcggc gacaccctg 
      atggtgaac cggctggcg gtaaaggcg gtgcgacgg 
      gagcacctc aactccacg tacaagtgc caggccagc 
      aacacaaag ttgatgatg cccgcagag aagactgta 
      agactagaa cttctcctg cggcctctc agtgttgtc 
      attccttac aaaccccgg caaatggta gccaaacag 
      gactacacc atacaatgc caagtgacg gggtcaagg 
      cctaaggcc acggtgacc tggacgaga gacaatcgg 
      gacttcagg agaggaaag tttttggag gagggcaac 
      gagactgtg gtaataagc tcggtgacg ttctccccg 
      gagcccgaa gacgacggc acattcctc aagtgtctg 
      ggtgacaac ccgaagctg cccggcttc agccaggaa 
      gactcgttc aaactgaac gtcgtctcc tgtggcgaa 
      atcttcact tcaaatggg tattcgcac ggccccgac 
      gtaaggcaa cactttaag cagacatcg gtggaaaaa 
      gaaaccgac cgccgttta tttgccgat gtgtattgg 
      gagaaccgg aacgaataa
    mRNA sequence
    • >TC033798 RA
      ggaattttc cggtcgggg tttcctccc ttaactcac 
      agaatgcct ggcgtcagt aacgtttcg gtgttgagc 
      aggggtggt tttttcgcg ccactttga cgcggactc 
      gggttacgt tccgtcaat gacataatc aacaatttg 
      gcgagattt cactcccgg attcgggac gactctgtg 
      cgcgattct gtgaaactg tgaggtgaa aatgcgttg 
      ggttctcgt ttttggggc gatttgctg ctgtgtgac 
      ttgtcagac gttggtgcc cgacgagga ggaagcagc 
      gatcgtctc atcggtgga cgcagttct gggtcggag 
      cgccacact accatgtga catcgaccc aggcatcag 
      ggaagaccg ggtgtacat ggtgctgtg gtacagaga 
      cacccacac caagcccat ctacagctt cgacgtccg 
      cggccgccc tttcaacaa agcgatcca ctggtcgga 
      ccccatggt gttcggccc ccgagctta cttcgtgac 
      ggtctcaaa gcccgccgc cctcaccct ggacggggt 
      gactctgaa cgacgaagg catctaccg atgtcgggt 
      ggatttccg cacaacgcc gaccaggaa tttcgccat 
      caatcttac ggtcattgt tcctccgca ccaggtgct 
      ggtgtacga taactccgg acgggacat cgttgggat 
      cgtgggtcc tctggagga gggtgcgga cctggtgct 
      aacttgcga ggttcgagg agggcggcc gacgccgac 
      ggtctcctg gttcatgaa cgagcggct ggtcgaggg 
      ggacgtgga ggccatcgg cgacaccct gatggtgaa 
      ccggctggc ggtaaaggc ggtgcgacg ggagcacct 
      caactccac gtacaagtg ccaggccag caacacaaa 
      gttgatgat gcccgcaga gaagactgt aagactaga 
      acttctcct gcggcctct cagtgttgt cattcctta 
      caaaccccg gcaaatggt agccaaaca ggactacac 
      catacaatg ccaagtgac ggggtcaag gcctaaggc 
      cacggtgac ctggacgag agacaatcg ggacttcag 
      gagaggaaa gtttttgga ggagggcaa cgagactgt 
      ggtaataag ctcggtgac gttctcccc ggagcccga 
      agacgacgg cacattcct caagtgtct gggtgacaa 
      cccgaagct gcccggctt cagccagga agactcgtt 
      caaactgaa cgtcgtctc ctgtggcga aatcttcac 
      ttcaaatgg gtattcgca cggccccga cgtaaggca 
      acactttaa gcagacatc ggtggaaaa agaaaccga 
      ccgccgttt atttgccga tgtgtattg ggagaaccg 
      gaacgaata agtatcgat tttgaacct ggctaacgc 
      gaaatcgct tattcgacg gcgcgagtg tgtgctttt 
      caaatttcg aacgactcg gtaattgga aatttccgc 
      ttcatttcg ctacgcaat tttataaaa tttatttaa 
      cacgttgta attattaag cgccacgtc aaaacggga 
      ccgaattgc gttgccgga tttattgag gcactcatt 
      gtgatcgct tcctcagca gaaaatcca agctttccg 
      gctccacga taagtcaag atgcgataa tttcaattg 
      caaccggat taccaaagt ctttttaca actttcgat 
      tcggtacat aagccatgc cgaaacatg atctaaaat 
      tccctttag caactatgt aaaagtcag tacaggaac 
      aagcaatca aacagcatt tttttattc ccattaaat 
      aaatatttc attaatcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY