Status
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Gene information
General information
TC033790
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008831
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10548854..10556881
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033790
    iBeetle screen iB_02865

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033790 RA
      MTEEKHKRH GMNGNADGV IKDEPPKTS YVQGGRLKF 
      FKDGKFILE LERAREGER VSWVSVPRK TFWPPQGTA 
      ASTPSYRQE SSASLSVSD DNSSIQSSP WQRDHSWKQ 
      PSPRRNQSK ELVFFFWRP KHKRSFSKI RKSRRPYTE 
      IVDEVGVVN ERTSKDEVS NNNCKSRVR ASLLVIVQN 
      LSEKAAGGG AGRGDVVVS PRKRFLREM EKDKVQSED 
      GCQKRSRNK TPQVTAKIG SPVSANGVT EDVKPARNC 
      SYSITSLLA EDRNVKRSP SSSPSHFNT VTQSQYCSP 
      SSEDRWYSE SVDRLRSIE LSQADKRGY PPYPHPSYL 
      PHYMYPYPL SPYYGPGVY GRGYVMPPV YQAPPMSIT 
      MRHDTPSCS WAADPPRDL ERRDDNITD MPLNLSKHA 
      G
    CDS sequence
    • >TC033790 RA
      atgacagaa gaaaagcac aaaagacat ggcatgaat 
      ggaaatgct gatggggtt atcaaagac gagcctccg 
      aagaccagc tatgtgcag ggagggcga ctcaagttc 
      ttcaaagat ggaaaattt attctggaa ctggagagg 
      gccagagag ggcgaacgg gtctcgtgg gtctccgtg 
      cccaggaaa acattctgg ccaccgcaa ggcactgcc 
      gcctccacg ccctcgtac cgacaagaa agcagtgct 
      tcgctgagt gtttcagac gacaacagt tcgatccag 
      tcgtcgcct tggcagcgc gaccactcg tggaagcag 
      ccgtcgccg cgccgcaac cagtcgaaa gagttggtg 
      ttctttttc tggcgtccg aaacacaaa cgttcattt 
      agcaaaatt cgaaagtca cgtcgtccg tacacagaa 
      atcgtcgac gaagtgggt gttgtgaat gaaaggact 
      tcgaaagat gaggtgtca aataacaat tgcaagagt 
      cgcgtgcgt gcttccttg ctcgtcatc gtgcaaaac 
      ctgtcggag aaggcggcg ggcggcggt gcgggccgc 
      ggcgacgtc gtcgtctct ccgcgaaaa cgcttcttg 
      cgcgaaatg gaaaaggac aaggtgcaa tcggaggac 
      ggttgccag aagagaagc aggaacaag acgccacag 
      gtgactgcc aaaattggc agtccggtt agtgcgaac 
      ggggttaca gaagacgtg aagccggcg cgcaattgc 
      agctacagc atcacttcc ttgttggct gaagatcgt 
      aacgttaag cggtcacct agcagctct cctagtcac 
      tttaatact gtgacgcaa tcgcagtac tgttcgcct 
      tcctcagag gataggtgg tattcggaa agcgttgat 
      aggttgcgt tccattgaa ctctcacag gccgataaa 
      agaggttat cctccttat ccccatcct tcatactta 
      ccccattat atgtaccct taccctcta tcaccatac 
      tatggtccc ggcgtttac ggaagaggt tacgttatg 
      ccgcctgtc taccaggct ccccccatg tccataaca 
      atgcgtcat gatactcct tcctgttcc tgggcagcg 
      gaccctccc agggattta gaacgtcgg gatgataat 
      attacagat atgccgttg aatctgtct aaacacgcc 
      ggttga
    mRNA sequence
    • >TC033790 RA
      taaggaggc aagctgctg gtgtacgtt tgtcgatgt 
      tgtcgttcg tcggtgcgg aataaacaa gtggtgtgt 
      gcggtaaat aatgcccga aatatgagg aaggcgtat 
      ttagtacga tgtgatctc gagcgtgta ccatcagca 
      gacgcagtg gaggtgaaa ttgcgagaa cggttcgcg 
      gtagtgtca gcaggtgga atggaatga aacatcaca 
      ttcgaagaa ttgggcaat ttaaagatg gtaacttgc 
      aacgtatgg acccgaatt ccgcaggaa acgctatga 
      gatgcatat atccgaatc acgccagtc ttcaaacac 
      catcttgac aaaatgttc aaaaatgac agaagaaaa 
      gcacaaaag acatggcat gaatggaaa tgctgatgg 
      ggttatcaa agacgagcc tccgaagac cagctatgt 
      gcagggagg gcgactcaa gttcttcaa agatggaaa 
      atttattct ggaactgga gagggccag agagggcga 
      acgggtctc gtgggtctc cgtgcccag gaaaacatt 
      ctggccacc gcaaggcac tgccgcctc cacgccctc 
      gtaccgaca agaaagcag tgcttcgct gagtgtttc 
      agacgacaa cagttcgat ccagtcgtc gccttggca 
      gcgcgacca ctcgtggaa gcagccgtc gccgcgccg 
      caaccagtc gaaagagtt ggtgttctt tttctggcg 
      tccgaaaca caaacgttc atttagcaa aattcgaaa 
      gtcacgtcg tccgtacac agaaatcgt cgacgaagt 
      gggtgttgt gaatgaaag gacttcgaa agatgaggt 
      gtcaaataa caattgcaa gagtcgcgt gcgtgcttc 
      cttgctcgt catcgtgca aaacctgtc ggagaaggc 
      ggcgggcgg cggtgcggg ccgcggcga cgtcgtcgt 
      ctctccgcg aaaacgctt cttgcgcga aatggaaaa 
      ggacaaggt gcaatcgga ggacggttg ccagaagag 
      aagcaggaa caagacgcc acaggtgac tgccaaaat 
      tggcagtcc ggttagtgc gaacggggt tacagaaga 
      cgtgaagcc ggcgcgcaa ttgcagcta cagcatcac 
      ttccttgtt ggctgaaga tcgtaacgt taagcggtc 
      acctagcag ctctcctag tcactttaa tactgtgac 
      gcaatcgca gtactgttc gccttcctc agaggatag 
      gtggtattc ggaaagcgt tgataggtt gcgttccat 
      tgaactctc acaggccga taaaagagg ttatcctcc 
      ttatcccca tccttcata cttacccca ttatatgta 
      cccttaccc tctatcacc atactatgg tcccggcgt 
      ttacggaag aggttacgt tatgccgcc tgtctacca 
      ggctccccc catgtccat aacaatgcg tcatgatac 
      tccttcctg ttcctgggc agcggaccc tcccaggga 
      tttagaacg tcgggatga taatattac agatatgcc 
      gttgaatct gtctaaaca cgccggttg aaccggaca 
      cgtgccttc catttgtta caactatat caatatttt 
      tatattaat taaagtaat ttttattct catatcgtt 
      ctattaaac gaaggaaca agacggcat tcggaattt 
      tttttcaat attttgatg ttgttattt tgttgtgtg 
      atacatatt ttgtgggta aatacttgt tgttttgga 
      aaaataata aaacgaaaa aatttagaa aagtctgtg 
      tggttttca tgtgtaaaa atagtaaat atttatagc 
      tttaaagca tactattgt gcaattgac agctgaaca 
      agcaaaaag tgtaattat gattggagg tgtattcga 
      aattttcta aattcgctt ctgttttat ttggtatat 
      tttaatagt tagttttat atattttct ctttaatcg 
      ccaaatgta tatatcaac agtcatttt tgttatttt 
      tgtaaatat taaatatat tttagagtg ttaatttat 
      cagtgtgtt gccatgttg ctcgtactt gcttttgca 
      ttggaatcc tctcgtctt tgcgtaaaa cagcagcgt 
      tttaattgt tcattatta ttttaccat cgtttttat 
      tttaaacaa tgacaaatt tgatacata atacattaa 
      ttttcgctt ttttttaaa cactagtcc aaggttaag 
      gcatgtatg aacaactcg agatgtaaa tttattttg 
      cccatcaaa aaatttaaa gtattcatt ttatgcagt 
      acaaccttc aaattattt taatgaatt gttcaagtt 
      ccttttctt atggtctaa aatgtaatt ttgagaaat 
      taagttgtg taaatactt acgtgtgac attttacca 
      ttaagataa caaatttgt ataaatatc tatattttc 
      gtacggttc attcttctt gaaaagatt caacgcgta 
      atctttaaa agacaatag ataggtcta tgtaaaata 
      tattttttt taattagga gactctatt tatagtcag 
      gatatttat agatttatt tcttcatat ttaatacat 
      ttattatgt agagtattc aacatgttt ttgtgccat 
      ttgctatta acataatgt tcttcaaaa taatgtatt 
      tttttaatg gtgcgattt atgactaaa ttacgttaa 
      ggataactt atgaggaag ttacagtta taattattt 
      ttaatgcca aagcgagta ttaactctt ttgagatca 
      tttagttgc cttgtttta ttttcatgt taataacat 
      ttaacacta taactttat tggcactta acttattgt 
      gatatgagt aaatattga aacttgtat aattacaag 
      agtccaacc cttgaactg agtatttat acttgtatt 
      tctatgatt gattaaact ctacagtgt ata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY