Status
Please wait ...
Gene information
General information
TC031208 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 4069177..4076124
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031208
    iBeetle screen iB_02864
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031208 RA
      MSAEEVFSK LRSNSEQNC DEAVRLLVK IAENIVKDP 
      ANLKVRSLQ KSNSTIKNK ILSVKGGSE CLKLMGFQE 
      NESSFTLPS TTSMEKLQE VKKCLLNFK NDEKRQGAV 
      PKLKQETMI RSNSTNFEV NGDDLDNVK RFVLPTLKK 
      LFTNPFLQR IETEFHRAL AFDDKECQK RAKKLIPLD 
      RLEKNAQRN LRYVQTRIK NERVQDPEF SVQDMLLIE 
      LLKWFKEEF FSWVDSPGC EKCGGNTAM SHMSSDKTD 
      LVYTSRVEV YKCKTCNAF TKFPRFNDL NILLETRRG 
      RCGEWANVF TLLCRSLGW DARYVVDEL DHVWTEVYS 
      VTQKRWVHC DCCENACDT PLMYETGWN KKLTYIIAY 
      SPDEVQDVT WRYSSNHRE VRTRRNKCS EDELVNALI 
      TLRNERQKS FSAPKRDYL TKRLVTELI ELMVERKAN 
      DSEKFGRIS GSKEWRSAR GEIKNENMY VWIVPSKHI 
      VNNKLTIKY STAFDKYEF VKGDNSVLG VNGWQYGVY 
      DFSNVFRKE EKDWKTVYL ARNEGTDQG SISWRFELD 
      KGVKKCIDN LSLKFNYQL YESGVVQIQ LISDDKCVD 
      FPKTEGALE TQAFSGVRH FTIKATLSG GKGNVAWQH 
      AQLFRQSSD SKDFPFVLS VTFRNVE
    CDS sequence
    • >TC031208 RA
      atgagtgca gaagaagtg ttttcgaag ctaagatcc 
      aactcggaa caaaactgt gatgaggct gtacgtctc 
      ctagttaaa atagccgaa aatattgtt aaagatcca 
      gctaaccta aaagtacga tctctacaa aaaagcaat 
      tccacaata aaaaataaa attttgtcc gtgaaggga 
      ggttcggaa tgtttgaaa ttaatgggc tttcaagag 
      aatgagtct agtttcaca ctaccttca acaacttca 
      atggaaaaa ttacaagaa gtaaaaaaa tgtctcttg 
      aattttaaa aatgatgag aaacgccag ggggctgtg 
      cctaaactg aagcaagaa actatgatc agaagtaat 
      agcaccaat ttcgaagtt aatggcgat gatttagat 
      aatgtgaag agatttgtt ttacctacc cttaaaaag 
      ctctttacc aaccctttt ttgcaaagg atcgaaacg 
      gaattccat cgtgcactc gctttcgac gataaagaa 
      tgtcaaaaa cgtgccaaa aagctcatt ccattggac 
      aggcttgaa aaaaatgca cagagaaat ttgcgttat 
      gtccaaacc cgcataaaa aacgaaaga gtccaagac 
      ccggagttt tccgtccag gatatgtta ctcatagaa 
      ttgttaaaa tggttcaaa gaagagttt ttctcgtgg 
      gttgattcc cccggctgt gagaaatgc gggggaaac 
      actgcgatg tctcacatg agttcggat aagaccgat 
      ttagtttat acaagcagg gttgaagtc tacaaatgt 
      aaaacttgc aacgcattt actaaattt ccccgtttt 
      aacgattta aatatctta ttggagact cgaagaggg 
      aggtgtggg gaatgggca aatgttttt acgttactt 
      tgccgctct ttggggtgg gatgctcgc tatgtggtt 
      gatgaactt gaccacgtg tggactgaa gtttattca 
      gttacgcaa aaacgttgg gttcattgc gactgctgc 
      gaaaacgca tgtgatacg cccctaatg tacgaaaca 
      gggtggaat aaaaaatta acatacatt atagcgtat 
      tctcctgat gaagtccag gatgtcacc tggcgttat 
      tccagcaat cacagagaa gttcggaca cgaagaaac 
      aaatgttca gaagacgaa cttgtgaat gccttaatt 
      acgcttaga aatgagcgc cagaagtca tttagtgca 
      cctaaacgt gattattta acgaaaaga ttggttact 
      gagttgata gaattgatg gtggagaga aaagcgaat 
      gatagtgaa aagttcggc cgtatttcg ggatcgaaa 
      gaatggaga agtgcaaga ggggaaata aaaaatgag 
      aacatgtat gtgtggatt gttccgagt aaacatatc 
      gttaataat aaattgaca attaaatat tccaccgcg 
      tttgataag tacgagttt gtgaagggg gataatagt 
      gttttgggg gtaaacggc tggcaatat ggtgtctat 
      gatttcagt aatgtgttc agaaaagaa gagaaagat 
      tggaaaacg gtttatttg gcccgaaat gaagggacc 
      gatcaaggg agtatatca tggagattc gaattagat 
      aaaggggtt aaaaagtgt atagataac ttatcatta 
      aaattcaat tatcaacta tacgaaagt ggagtcgta 
      caaatacaa ctaatttcc gatgataaa tgtgtggat 
      tttccaaaa actgaaggg gctctagaa actcaggca 
      ttttcggga gtgaggcat tttacaatt aaggcaact 
      ttaagtgga ggcaagggc aatgtggcc tggcagcat 
      gcccaatta ttccgtcag tccagtgat agcaaagat 
      tttccattt gttctttct gtaactttt agaaacgtt 
      gagtaa
    mRNA sequence
    • >TC031208 RA
      tccaaaaaa aagaattaa tcagcggtt taactaatt 
      aatgtgtta aaatgagtg cagaagaag tgttttcga 
      agctaagat ccaactcgg aacaaaact gtgatgagg 
      ctgtacgtc tcctagtta aaatagccg aaaatattg 
      ttaaagatc cagctaacc taaaagtac gatctctac 
      aaaaaagca attccacaa taaaaaata aaattttgt 
      ccgtgaagg gaggttcgg aatgtttga aattaatgg 
      gctttcaag agaatgagt ctagtttca cactacctt 
      caacaactt caatggaaa aattacaag aagtaaaaa 
      aatgtctct tgaatttta aaaatgatg agaaacgcc 
      agggggctg tgcctaaac tgaagcaag aaactatga 
      tcagaagta atagcacca atttcgaag ttaatggcg 
      atgatttag ataatgtga agagatttg ttttaccta 
      cccttaaaa agctcttta ccaaccctt ttttgcaaa 
      ggatcgaaa cggaattcc atcgtgcac tcgctttcg 
      acgataaag aatgtcaaa aacgtgcca aaaagctca 
      ttccattgg acaggcttg aaaaaaatg cacagagaa 
      atttgcgtt atgtccaaa cccgcataa aaaacgaaa 
      gagtccaag acccggagt tttccgtcc aggatatgt 
      tactcatag aattgttaa aatggttca aagaagagt 
      ttttctcgt gggttgatt cccccggct gtgagaaat 
      gcgggggaa acactgcga tgtctcaca tgagttcgg 
      ataagaccg atttagttt atacaagca gggttgaag 
      tctacaaat gtaaaactt gcaacgcat ttactaaat 
      ttccccgtt ttaacgatt taaatatct tattggaga 
      ctcgaagag ggaggtgtg gggaatggg caaatgttt 
      ttacgttac tttgccgct ctttggggt gggatgctc 
      gctatgtgg ttgatgaac ttgaccacg tgtggactg 
      aagtttatt cagttacgc aaaaacgtt gggttcatt 
      gcgactgct gcgaaaacg catgtgata cgcccctaa 
      tgtacgaaa cagggtgga ataaaaaat taacataca 
      ttatagcgt attctcctg atgaagtcc aggatgtca 
      cctggcgtt attccagca atcacagag aagttcgga 
      cacgaagaa acaaatgtt cagaagacg aacttgtga 
      atgccttaa ttacgctta gaaatgagc gccagaagt 
      catttagtg cacctaaac gtgattatt taacgaaaa 
      gattggtta ctgagttga tagaattga tggtggaga 
      gaaaagcga atgatagtg aaaagttcg gccgtattt 
      cgggatcga aagaatgga gaagtgcaa gaggggaaa 
      taaaaaatg agaacatgt atgtgtgga ttgttccga 
      gtaaacata tcgttaata ataaattga caattaaat 
      attccaccg cgtttgata agtacgagt ttgtgaagg 
      gggataata gtgttttgg gggtaaacg gctggcaat 
      atggtgtct atgatttca gtaatgtgt tcagaaaag 
      aagagaaag attggaaaa cggtttatt tggcccgaa 
      atgaaggga ccgatcaag ggagtatat catggagat 
      tcgaattag ataaagggg ttaaaaagt gtatagata 
      acttatcat taaaattca attatcaac tatacgaaa 
      gtggagtcg tacaaatac aactaattt ccgatgata 
      aatgtgtgg attttccaa aaactgaag gggctctag 
      aaactcagg cattttcgg gagtgaggc attttacaa 
      ttaaggcaa ctttaagtg gaggcaagg gcaatgtgg 
      cctggcagc atgcccaat tattccgtc agtccagtg 
      atagcaaag attttccat ttgttcttt ctgtaactt 
      ttagaaacg ttgagtaaa taaatattt gttacaatt 
      tctgttttg tatt