Status
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Gene information
General information
TC033770
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009961,TC009962
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11294073..11298529
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033770
    iBeetle screen iB_02863

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033770 RA
      MMALNEDKK TVIQDFKLD QDNELRFEV ESKNEKVYV 
      TLKSGKAEV FGTELVKGK TYEFTSGAK VAVYTWHGC 
      TIEVKGKTD VSYVAKETP MVTYSNCHA ALEFMRIEA 
      ERENKKGPT VMLVGPNDV GKSTVCRIL LNYAVRMGR 
      RPIFVDLDV GQGQISIPG TIGALLIER PASIDEGFS 
      QEAPLVYHT GHKSPQPNI ALYSMLVTQ LANTVKDRL 
      EVNKKTRAS GVIINTCGW IKGTGYKQI LHSAKAFEV 
      DVILVLDQE RLYNELVRD MPNFVKVIF LQKSGGVVE 
      RSKSVRSEA RDQRIREYF YGTPKNSMY PHSFDVKWS 
      EIKIYKIGA PALPDSCLP LGMKAEDHL TKLVPVTPN 
      PGILHHLLA VSFSEGEDE DIISSHVAG FVCVTNVDT 
      DRQIVTLLS PQPKPLPNN ILLLSELQF MDSDEPAQV 
      TGEQIAQPS EEIKEEQNE ETQEPQIET EIKIEDLAS 
      IEPSLLASI DTAPSLLPS IKESDQVTE SEQVVIPAE 
      PQEPEINED SMEAKLVLL RQFSSDYFL YKHSPSFKC 
      FEQALCSQS DLRLTLYFF PPLTDPGLD EAFNYIPPE 
      EEHDPDGAD KYINMCKDL AIVPISRVI RSLKTDTLN 
      LKYYGLTLK QMRGIADAL KVNSNIKYL IFEDNWMSP 
      EATALIGEV LRESNSIRF LNLKECRIG EDGAEKLCE 
      GISSTQFLQ ELDLSYNSL GDKGLKILQ PSLIENTSI 
      KKLNISHNN LTEDSGATL EAILLENKY LEELDLSWN 
      GFFTAPGNK KLCNGLAKN ELIKWLNLS WNGIGTGPA 
      MRPITKYLR KTQVLQFLD LSWNRITQR SLILLRAAL 
      IRNKSLNGI KLGHNIYTP DEAYFLASL FSRVKNNNF 
      TFLDMEDMC VNKDFLPLK RKFQREGRQ IPHGHVLSN 
      YEIYGPDIN KLLFQRCRY LAMKPKKKK KKKDFGHFV 
      LSLPDKNVT PQEFQELLK KKKIKKIDK DLLNELMAR 
      FQTKKKKID SVAMKADYM RLYPDTVLP EEKKKKKKK 
      KKRRKGKKP KKKE
    CDS sequence
    • >TC033770 RA
      atgatggca ttaaacgaa gacaagaaa actgtaatt 
      caagacttt aaactggac caagacaac gagctccgc 
      ttcgaagtt gaatccaaa aatgaaaaa gtctacgtc 
      acactcaaa agcggaaag gccgaagta ttcggtacc 
      gaactggtc aaaggcaag acgtacgag ttcacatct 
      ggggccaaa gttgctgtg tacacctgg cacggctgt 
      actatcgaa gtcaaagga aaaaccgac gtgagttac 
      gtggcgaaa gagacccca atggtcact tattcgaac 
      tgtcacgcg gctctagaa ttcatgcga attgaggca 
      gagcgggag aacaaaaag ggccccact gtgatgcta 
      gtggggcca aatgatgtt ggtaaatca acggtgtgc 
      cgcattttg cttaactat gccgtgagg atggggcgg 
      aggcccatt ttcgtggat ctggatgtg gggcaagga 
      cagatttca attccgggg accataggt gccctactt 
      attgaaaga ccggcctct attgatgag gggttttca 
      caagaggcc ccacttgtg taccacacg gggcataaa 
      tcgccgcaa ccgaacatc gcactttat tccatgtta 
      gtgacacaa cttgcaaac acagtaaaa gacaggctc 
      gaagtcaac aaaaaaacc agggcgtct ggtgttatt 
      attaacact tgtggctgg attaagggc actggatac 
      aaacaaatc ctccactcg gcgaaagct ttcgaagtt 
      gatgttatt ttagttctg gaccaggaa aggctttac 
      aatgagtta gtgcgtgat atgccaaat tttgttaaa 
      gtcatattt ttacaaaaa agtggtggc gtggttgaa 
      cgctcaaaa agtgttaga tctgaagca agagatcaa 
      cgaattcgg gaatatttc tacggaacc cccaaaaat 
      tcaatgtat ccacactcg tttgacgtc aagtggagc 
      gaaattaaa atttacaaa attggggcg ccagctttg 
      ccagactcg tgcctgcct ttgggtatg aaggcagag 
      gatcatctg acgaaatta gtgccagtg acgccaaat 
      ccggggatt ttgcatcat ctacttgca gtcagtttt 
      tcagaggga gaagatgaa gatatcatc tcctcacac 
      gtcgccgga ttcgtttgt gtaaccaac gtggacacg 
      gatcgccaa atcgttacg ctgttatcg cctcagccg 
      aaaccatta cctaacaac atacttctt ctgtcagag 
      ttacagttc atggacagt gacgaacca gcgcaagtc 
      actggtgaa caaatcgca caaccatct gaagaaatc 
      aaagaagag cagaatgaa gaaactcaa gaaccacaa 
      attgaaact gaaataaaa atagaagat ctcgcaagc 
      atagaacct agtctgctc gcgagcatc gacacagca 
      ccaagtctg ctcccgagc attaaagag agtgaccaa 
      gtcactgaa agtgagcaa gtggttata ccagcagaa 
      ccccaggaa cctgaaata aatgaagat tcaatggaa 
      gctaaacta gttctcctg cggcagttt tcaagcgac 
      tatttcttg tacaaacac agcccttct ttcaaatgt 
      ttcgaacag gccctgtgc tcacagagc gacctccga 
      ctgactttg tattttttt ccaccgctc actgacccc 
      ggcttggat gaagccttc aactacata ccgcctgag 
      gaagagcat gaccctgac ggtgctgat aagtacatc 
      aacatgtgc aaagacttg gccattgtg cccatttct 
      cgcgtgata agatcacta aaaacggac actttaaat 
      ttaaagtat tacggactg actttaaaa caaatgagg 
      ggcatagca gacgcgttg aaggtgaac agtaacatc 
      aagtattta atttttgaa gacaattgg atgtcgccg 
      gaggcaacg gccctcatt ggcgaggtt ctacgtgaa 
      agtaactcg attcggttt ttaaatttg aaagagtgc 
      cgaattggc gaagacggg gctgaaaaa ttgtgcgaa 
      ggcatatca tcaacgcag ttcttgcaa gaactggat 
      ttaagttat aacagtttg ggggataaa ggtttgaaa 
      atacttcag cccagttta attgaaaat acatcgatt 
      aaaaaacta aatatcagt cataataat ttaactgag 
      gactcgggg gcgactttg gaggcaatt ttgttagaa 
      aataaatat ttggaagag ttagatctc tcctggaat 
      ggatttttc acagcccct gggaataaa aaactgtgc 
      aacggtttg gctaaaaat gaattaatt aaatggtta 
      aatctgtct tggaatggg attggcaca ggtcctgca 
      atgcggccc atcacaaaa tacttgcgg aagactcaa 
      gtgttacag tttttagat ttgagctgg aacagaata 
      acacagcgt agtttaatc ttattgaga gcggcttta 
      atacgaaac aagtcactg aatggaatt aagctggga 
      cataatatt tacactcct gacgaagct tactttcta 
      gcgtcgctt ttttcaaga gtaaaaaac aataatttt 
      acatttttg gacatggaa gacatgtgc gttaacaaa 
      gattttttg ccgttgaaa cgaaaattt caaagagaa 
      ggcagacaa atcccccat gggcatgtt ttaagcaat 
      tatgagatt tacggtcct gatataaac aaactgctc 
      tttcagcga tgtcgctat ttagcaatg aagccaaag 
      aagaagaag aaaaagaaa gactttgga cattttgta 
      ttgtcgctg cctgataaa aatgtgaca ccgcaagag 
      ttccaagaa ttgttgaaa aagaagaaa atcaaaaaa 
      attgacaag gacttactc aatgaatta atggcaagg 
      tttcaaacc aagaaaaag aaaattgat tcagttgcc 
      atgaaagcc gattacatg agactgtac cccgacacg 
      gtactacct gaagaaaag aagaagaaa aagaagaag 
      aagaagagg agaaaaggc aagaaaccg aaaaagaag 
      gaataa
    mRNA sequence
    • >TC033770 RA
      tttgtgttt aatttattt ttattgtct tgttacaaa 
      ctgtagtcg aaaaagatg tcataaagt tttatgatg 
      atggcatta aacgaagac aagaaaact gtaattcaa 
      gactttaaa ctggaccaa gacaacgag ctccgcttc 
      gaagttgaa tccaaaaat gaaaaagtc tacgtcaca 
      ctcaaaagc ggaaaggcc gaagtattc ggtaccgaa 
      ctggtcaaa ggcaagacg tacgagttc acatctggg 
      gccaaagtt gctgtgtac acctggcac ggctgtact 
      atcgaagtc aaaggaaaa accgacgtg agttacgtg 
      gcgaaagag accccaatg gtcacttat tcgaactgt 
      cacgcggct ctagaattc atgcgaatt gaggcagag 
      cgggagaac aaaaagggc cccactgtg atgctagtg 
      gggccaaat gatgttggt aaatcaacg gtgtgccgc 
      attttgctt aactatgcc gtgaggatg gggcggagg 
      cccattttc gtggatctg gatgtgggg caaggacag 
      atttcaatt ccggggacc ataggtgcc ctacttatt 
      gaaagaccg gcctctatt gatgagggg ttttcacaa 
      gaggcccca cttgtgtac cacacgggg cataaatcg 
      ccgcaaccg aacatcgca ctttattcc atgttagtg 
      acacaactt gcaaacaca gtaaaagac aggctcgaa 
      gtcaacaaa aaaaccagg gcgtctggt gttattatt 
      aacacttgt ggctggatt aagggcact ggatacaaa 
      caaatcctc cactcggcg aaagctttc gaagttgat 
      gttatttta gttctggac caggaaagg ctttacaat 
      gagttagtg cgtgatatg ccaaatttt gttaaagtc 
      atattttta caaaaaagt ggtggcgtg gttgaacgc 
      tcaaaaagt gttagatct gaagcaaga gatcaacga 
      attcgggaa tatttctac ggaaccccc aaaaattca 
      atgtatcca cactcgttt gacgtcaag tggagcgaa 
      attaaaatt tacaaaatt ggggcgcca gctttgcca 
      gactcgtgc ctgcctttg ggtatgaag gcagaggat 
      catctgacg aaattagtg ccagtgacg ccaaatccg 
      gggattttg catcatcta cttgcagtc agtttttca 
      gagggagaa gatgaagat atcatctcc tcacacgtc 
      gccggattc gtttgtgta accaacgtg gacacggat 
      cgccaaatc gttacgctg ttatcgcct cagccgaaa 
      ccattacct aacaacata cttcttctg tcagagtta 
      cagttcatg gacagtgac gaaccagcg caagtcact 
      ggtgaacaa atcgcacaa ccatctgaa gaaatcaaa 
      gaagagcag aatgaagaa actcaagaa ccacaaatt 
      gaaactgaa ataaaaata gaagatctc gcaagcata 
      gaacctagt ctgctcgcg agcatcgac acagcacca 
      agtctgctc ccgagcatt aaagagagt gaccaagtc 
      actgaaagt gagcaagtg gttatacca gcagaaccc 
      caggaacct gaaataaat gaagattca atggaagct 
      aaactagtt ctcctgcgg cagttttca agcgactat 
      ttcttgtac aaacacagc ccttctttc aaatgtttc 
      gaacaggcc ctgtgctca cagagcgac ctccgactg 
      actttgtat ttttttcca ccgctcact gaccccggc 
      ttggatgaa gccttcaac tacataccg cctgaggaa 
      gagcatgac cctgacggt gctgataag tacatcaac 
      atgtgcaaa gacttggcc attgtgccc atttctcgc 
      gtgataaga tcactaaaa acggacact ttaaattta 
      aagtattac ggactgact ttaaaacaa atgaggggc 
      atagcagac gcgttgaag gtgaacagt aacatcaag 
      tatttaatt tttgaagac aattggatg tcgccggag 
      gcaacggcc ctcattggc gaggttcta cgtgaaagt 
      aactcgatt cggttttta aatttgaaa gagtgccga 
      attggcgaa gacggggct gaaaaattg tgcgaaggc 
      atatcatca acgcagttc ttgcaagaa ctggattta 
      agttataac agtttgggg gataaaggt ttgaaaata 
      cttcagccc agtttaatt gaaaataca tcgattaaa 
      aaactaaat atcagtcat aataattta actgaggac 
      tcgggggcg actttggag gcaattttg ttagaaaat 
      aaatatttg gaagagtta gatctctcc tggaatgga 
      tttttcaca gcccctggg aataaaaaa ctgtgcaac 
      ggtttggct aaaaatgaa ttaattaaa tggttaaat 
      ctgtcttgg aatgggatt ggcacaggt cctgcaatg 
      cggcccatc acaaaatac ttgcggaag actcaagtg 
      ttacagttt ttagatttg agctggaac agaataaca 
      cagcgtagt ttaatctta ttgagagcg gctttaata 
      cgaaacaag tcactgaat ggaattaag ctgggacat 
      aatatttac actcctgac gaagcttac tttctagcg 
      tcgcttttt tcaagagta aaaaacaat aattttaca 
      tttttggac atggaagac atgtgcgtt aacaaagat 
      tttttgccg ttgaaacga aaatttcaa agagaaggc 
      agacaaatc ccccatggg catgtttta agcaattat 
      gagatttac ggtcctgat ataaacaaa ctgctcttt 
      cagcgatgt cgctattta gcaatgaag ccaaagaag 
      aagaagaaa aagaaagac tttggacat tttgtattg 
      tcgctgcct gataaaaat gtgacaccg caagagttc 
      caagaattg ttgaaaaag aagaaaatc aaaaaaatt 
      gacaaggac ttactcaat gaattaatg gcaaggttt 
      caaaccaag aaaaagaaa attgattca gttgccatg 
      aaagccgat tacatgaga ctgtacccc gacacggta 
      ctacctgaa gaaaagaag aagaaaaag aagaagaag 
      aagaggaga aaaggcaag aaaccgaaa aagaaggaa 
      taattaaaa tacacaaat aaaaatcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY