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Gene information
General information
TC033746 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 12087916..12098948
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033746
    iBeetle screen iB_02862

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Adult morphology
    wings shortened - Number of animals showing the phenotype: 3

    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovariole decreased - Number of animals showing the phenotype: 2

    tropharium size decreased - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033746 RA
      MSWIPPNIA ESCAVYTNL TSCQPPTFM FLQLITQLF 
      GQSLDSEYD IYGQSGYDF GSNIYGYPG GGGGGSDEY 
      HQPRGREYD FIVLGAGSA GCVLANRLT EIPSWSVLL 
      LEAGDEEPE VADVPAFAP VLQQSSIDW GFSTQPDPN 
      SCLARQNGQ CSWARGKVM GGSSTINYM IYIRGNPRD 
      YDEWAEAGN PGWSWREVL PYFMKSEDN HNIDTVERQ 
      AHGVGGYLS VERFQFQEN NVRSLFEAF QELGLPVVD 
      QNAGRQIGT MMLQTTTRS GRRESANLA FIRPIRRKR 
      KNLTIETKA YIIRVLIDP HTKVAYGVE YEKNGKLFQ 
      ARARKEVLV TCGTIMTPK VLMLSGVGP AQHLQNLGI 
      QVIKDLPVG YNLMDHPTI DGVMFQISN ESATLVEPE 
      QITRDVFYY REEQAGPLS STGPLQVNT FVQTKYELE 
      PGRPDIQYS IDTANVVDY VTDLILAST TKVSPLSYY 
      NGFIIRPIL LNPVSRGVI KLNSTDPIY GYPIIYANT 
      FNEQIDALT MVEGIKQSL NLLKTRAMQ RMGVSLITT 
      PVAACDGYS FGTEDYWLC LVRSYTSTM YHYAGTCKM 
      GPKHDPFAV VDPKLRVYG IKNLRVIDT SIMPRVTRG 
      NTNAPTIMI AEKGADFIK ETWLKKKFS LPKIPKIPQ 
      IPKMDFKQM IKTFFFNH
    CDS sequence
    • >TC033746 RA
      atgtcttgg atacctccg aatatagca gaatcatgt 
      gcggtgtac accaacctc accagttgt cagccgccg 
      acgttcatg ttcctgcag ctcatcacg caattattt 
      ggacagtcg cttgactcc gaatacgac atttacggg 
      caaagtggt tacgatttt ggttcaaat atttacggg 
      taccccggc ggcggcggc ggcggcagt gacgagtac 
      catcagccc agagggcga gagtacgat ttcattgtg 
      ctaggtgca ggaagtgca ggatgtgtg ctggctaac 
      cgacttacg gagataccc tcatggagc gttctgctg 
      ctggaagct ggagacgag gagccggag gtagcggac 
      gtgcccgcc ttcgcgccc gtcttgcag cagtccagt 
      atcgattgg ggcttttcg acgcaacct gaccccaac 
      agttgtcta gcccgccag aacgggcaa tgttcatgg 
      gcgagaggc aaagtgatg ggtggctcc tccacgatc 
      aactacatg atctacatc cggggaaac ccgcgggat 
      tacgacgaa tgggccgaa gcgggcaac cctgggtgg 
      tcctggcgg gaagtcctc ccttacttc atgaagtcg 
      gaagataac cacaacatt gacaccgtc gagcgccag 
      gcccacggg gttggtggt tacctcagc gtggaacga 
      ttccagttc caggaaaac aacgtgcgt tcgcttttc 
      gaagctttc caagagttg gggttgcct gtggttgac 
      caaaacgcc ggcaggcag attggaacc atgatgctt 
      caaaccacc acaaggagt ggacggcgg gagagcgcg 
      aatctggcg tttatccga ccgattagg aggaagcga 
      aagaatttg accattgag accaaggcg tacattatc 
      cgagtgctg attgacccg cacaccaag gtcgcgtat 
      ggtgtggag tacgagaag aatggtaaa ttgttccag 
      gcgagagca aggaaagag gttcttgtc acatgtggt 
      acgatcatg acgcccaaa gtcctgatg ttgagtgga 
      gttgggcca gcgcaacat ttgcaaaat ttggggatc 
      caggtcatt aaggacctc ccagtgggt tacaatttg 
      atggatcac cccaccatc gacggtgtt atgttccaa 
      ataagcaac gaatcggcg actcttgtc gaaccggag 
      caaataact cgcgatgtt ttctactac agggaggaa 
      caagcgggt cctttatcc tcgacaggt cctttacaa 
      gtaaacact ttcgtgcaa acgaagtac gaactagaa 
      ccaggccga cccgacatc caatattcc attgacacc 
      gccaatgtc gtggactac gtcactgac ctcattcta 
      gcctccacg accaaagtt agccccctt tcctattac 
      aacggcttt atcatccgt ccgattttg ctcaaccct 
      gtaagcaga ggcgtcatc aagctgaat agcactgac 
      cctatctac ggctacccg ataatttac gcaaacacg 
      tttaacgaa caaatcgac gccttgacc atggttgag 
      ggaattaag caaagtttg aatttgctc aaaactcgc 
      gccatgcaa agaatgggc gtgagcttg attaccact 
      ccggtggcc gcctgtgat ggttacagc tttggtact 
      gaggactac tggttgtgc ttagtccgg tcctacact 
      agcaccatg taccattat gctggtacc tgcaaaatg 
      ggccccaaa cacgatcca tttgcggtg gtcgatccc 
      aagttgcga gtgtatggc atcaaaaat ttgagagtt 
      atcgacacc tccattatg cccagggtt actagaggt 
      aacacaaac gctcccact attatgatc gctgagaaa 
      ggagccgat tttatcaag gagacgtgg ttgaagaag 
      aagttttcg cttccgaaa attccaaaa atcccgcaa 
      atcccgaaa atggatttt aaacaaatg attaaaacg 
      tttttcttt aatcattaa
    mRNA sequence
    • >TC033746 RA
      ttatttcca tttcttgca ttgtgacag ggaagtttg 
      ttgatggaa acgtaatta gcatgtact ttctgcacc 
      tgatcggaa agcgcttag ttcagtgta cgccttagt 
      ggcagcatg tcttggata cctccgaat atagcagaa 
      tcatgtgcg gtgtacacc aacctcacc agttgtcag 
      ccgccgacg ttcatgttc ctgcagctc atcacgcaa 
      ttatttgga cagtcgctt gactccgaa tacgacatt 
      tacgggcaa agtggttac gattttggt tcaaatatt 
      tacgggtac cccggcggc ggcggcggc ggcagtgac 
      gagtaccat cagcccaga gggcgagag tacgatttc 
      attgtgcta ggtgcagga agtgcagga tgtgtgctg 
      gctaaccga cttacggag ataccctca tggagcgtt 
      ctgctgctg gaagctgga gacgaggag ccggaggta 
      gcggacgtg cccgccttc gcgcccgtc ttgcagcag 
      tccagtatc gattggggc ttttcgacg caacctgac 
      cccaacagt tgtctagcc cgccagaac gggcaatgt 
      tcatgggcg agaggcaaa gtgatgggt ggctcctcc 
      acgatcaac tacatgatc tacatccgg ggaaacccg 
      cgggattac gacgaatgg gccgaagcg ggcaaccct 
      gggtggtcc tggcgggaa gtcctccct tacttcatg 
      aagtcggaa gataaccac aacattgac accgtcgag 
      cgccaggcc cacggggtt ggtggttac ctcagcgtg 
      gaacgattc cagttccag gaaaacaac gtgcgttcg 
      cttttcgaa gctttccaa gagttgggg ttgcctgtg 
      gttgaccaa aacgccggc aggcagatt ggaaccatg 
      atgcttcaa accaccaca aggagtgga cggcgggag 
      agcgcgaat ctggcgttt atccgaccg attaggagg 
      aagcgaaag aatttgacc attgagacc aaggcgtac 
      attatccga gtgctgatt gacccgcac accaaggtc 
      gcgtatggt gtggagtac gagaagaat ggtaaattg 
      ttccaggcg agagcaagg aaagaggtt cttgtcaca 
      tgtggtacg atcatgacg cccaaagtc ctgatgttg 
      agtggagtt gggccagcg caacatttg caaaatttg 
      gggatccag gtcattaag gacctccca gtgggttac 
      aatttgatg gatcacccc accatcgac ggtgttatg 
      ttccaaata agcaacgaa tcggcgact cttgtcgaa 
      ccggagcaa ataactcgc gatgttttc tactacagg 
      gaggaacaa gcgggtcct ttatcctcg acaggtcct 
      ttacaagta aacactttc gtgcaaacg aagtacgaa 
      ctagaacca ggccgaccc gacatccaa tattccatt 
      gacaccgcc aatgtcgtg gactacgtc actgacctc 
      attctagcc tccacgacc aaagttagc cccctttcc 
      tattacaac ggctttatc atccgtccg attttgctc 
      aaccctgta agcagaggc gtcatcaag ctgaatagc 
      actgaccct atctacggc tacccgata atttacgca 
      aacacgttt aacgaacaa atcgacgcc ttgaccatg 
      gttgaggga attaagcaa agtttgaat ttgctcaaa 
      actcgcgcc atgcaaaga atgggcgtg agcttgatt 
      accactccg gtggccgcc tgtgatggt tacagcttt 
      ggtactgag gactactgg ttgtgctta gtccggtcc 
      tacactagc accatgtac cattatgct ggtacctgc 
      aaaatgggc cccaaacac gatccattt gcggtggtc 
      gatcccaag ttgcgagtg tatggcatc aaaaatttg 
      agagttatc gacacctcc attatgccc agggttact 
      agaggtaac acaaacgct cccactatt atgatcgct 
      gagaaagga gccgatttt atcaaggag acgtggttg 
      aagaagaag ttttcgctt ccgaaaatt ccaaaaatc 
      ccgcaaatc ccgaaaatg gattttaaa caaatgatt 
      aaaacgttt ttctttaat cattaactg aaagaggcc 
      taaaaagtg cctcgtctt ccgcttaca tcactacac 
      aaaacatta gatgcatta gaaatatct ataataatt 
      tttggtaat tataaattt tctatttac attcagaaa 
      ttgcactaa aattgttga tatttatgc tgcatctac 
      cttaccaac agatgggtc atactatcc agcaacata 
      taaaaagtg tggtaatag aaaagaaac gaaaaatcg 
      atcattttg tttggcctc agttctttg tttatattt 
      tttaagcaa cgttgcggt attcactta ccagaaacg 
      atcgtcagc gatgcaaaa agaatttac gttgagcaa 
      aagaaatca acggtttgt gttttctgg agtaattgc 
      aacttgaag ttaattgat tttaaaatt tgccattaa 
      tttgtaaac acttttcca gccttacca agcttttaa 
      caaaactcg gtagtaatg gcaaagtac tattttatt 
      tagtttcgg acagcactc taaacgact aacgccgtc 
      ttagtctat tttttagac tgcttttgt tgcaatttt 
      ctttccgaa atacttcca aataaaata atatttcga 
      tatccgtta agtgtacca aatcattta ccagttcga 
      acggatatc cgtgtttgt ttctattgt ttggtatga 
      aggttgaaa ttcgtctat tttgcacaa aacaattac 
      tgatccatc tgttaatta acttattta tttattcag 
      gtcttgtac aaaaatcga accgtttaa aacattatt 
      taacacaca atacatatt ttgttataa gtaacttat 
      ttttgtaga attgcttac cacagtagt taccaataa 
      taattcagg tttaataag tgggtacgt atgtagaag 
      agggtttat taaattaca acaagcttt tgtgaatat 
      cttttcagg ttcagcatg attttgaat caagcttac 
      ctaagcgac tttattgtt acaattttt atattatag 
      ctagaggta aagtctact gtgctaacg ttatgtagc 
      atagtagga gtaatatat ttttaagtc atgtagttg 
      tttctggtt acgcatgta tataataat ccttacatt 
      gttacatta agaagctac caaatgtgt tatcaagtc 
      aacattgta gtctaacta agcatcaag tagagtaga 
      tttgttaca gatcctatt gtattgtta taatccagt 
      cttgtaaat aaacaagtt tattaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY