Status
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Gene information
General information
TC008956 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 12690022..12702645
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008956
    iBeetle screen iB_02860

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008956 RA
      MSKLSFRAR ALDASKPMP IYMAEELPD LPDYSAINR 
      AVPQMPSGM EKEEECEHH LQRAICAGL IIPTPEVSD 
      MPDKEFYDK VYPANYKQP RQLIHMQPF TMEQDIPDY 
      DMDSEDERW LQSQAQRLD LTPLKFEEM MDRLEKSSG 
      QTVVNLNEA KALLKEDDD LIIAVFDYW LNKRLKTQH 
      PLILTVKTE QRAGAAPNN PYLAFRRRT EKMQTRKNR 
      KNDETSYEK MLKLRRDLH RAVTLLELV KKREKMKRE 
      HVYLTVETF EKRFHARDF SGAIYAEAA AIKTTRPAF 
      TPIFHNHYP NQSWSNKAI LKDEVIPRK EKRQYKKRK 
      HKSLGHTRS GGIGAAGLD GLGALSSDE DTMPQLSPE 
      PEELEDEGQ FAFRRNKLC SYHMPLPTE GNWPWCSKE 
      ENGLADKRF RFTLTSLSN PRRCIGFAR RRIGRGGRV 
      ILDRIGTDH DDFWRTLDF TIVEPNAKK EEQQNETNL 
      SSDGVVKMD ASFVNNNTN VNNNLRTDI KSEYGYVSN 
      SFSRTNVND VGGESESGG QIDVKKEVE EDGGPQPET 
      FAHSKSDED EMVEILRSV RRDWLHFRP KTPPPDEES 
      PCSLLPFSE SYFVPGANM SFSIEVQSL ETPSTLLDT 
      SAFLTDPFT FDNLDSIED VPVTDTRQS SDKIVQTGI 
      NLNVNVGLN TLTPSVNDN SSSVFSNSN ANLTDSIKL 
      EDATSSSTS DENDERTIG CSRFYVSDL GVTKKNQTS 
      KTQTVTSTL TSPKFNSVN AVSEGWSRS TATEGPLTN 
      TNGLLSQFG LDGRTKNNK ISYAYSGGV YQTVPPINA 
      SSGTLSLHT SAHTLTLNS HSANILEIP ITDDTEPVS 
      NKLVNSQCT EPGTATTNN KTKTIVRKN NLVMEVT
    CDS sequence
    • >TC008956 RA
      atgtccaag ctttcgttc agggcgagg gccctggac 
      gccagcaag cccatgccc atatacatg gcggaggag 
      ctcccggac ctcccggac tactcggcg atcaaccga 
      gcggtcccc cagatgccc tcgggcatg gaaaaggag 
      gaggaatgc gagcaccat ctgcaaagg gcgatatgt 
      gcggggctc atcatcccc acaccggaa gtttcggac 
      atgccagac aaggaattc tacgacaag gtttatccc 
      gccaactat aaacagccc cggcagctc atccacatg 
      caacccttc acgatggaa caagacatc cctgactac 
      gacatggac tcggaagac gaacgttgg ttgcaatcg 
      caggcgcag aggctggat ctgactcct ctaaagttc 
      gaggagatg atggaccgt ttggagaag agcagcggc 
      cagactgtg gtgaatctg aacgaggcc aaagctctg 
      ctgaaggag gatgacgat ctcataatc gcagtattc 
      gattattgg ttaaacaaa cgcctgaaa acccaacat 
      cccctaatc ctgacggtg aagacggaa cagagagcc 
      ggcgccgcc cccaacaac ccgtacctg gcgttccga 
      cggcgaacg gagaagatg cagacccgc aagaaccgc 
      aaaaacgac gaaacatcg tacgaaaaa atgctaaaa 
      cttcgtagg gatctccat cgggccgtc acgttgctc 
      gaactcgtc aagaaacgg gagaagatg aagcgggaa 
      cacgtctat ctcacagtg gaaacgttc gagaagaga 
      ttccatgcg agggacttc agtggtgcc atctatgcg 
      gaagcagca gcgatcaag acgacgagg ccggccttc 
      acaccgatc tttcacaat cactatccg aaccagagc 
      tggtcgaat aaggcgata ctcaaagac gaagttata 
      cccaggaaa gagaaaaga cagtataaa aagaggaaa 
      cacaaatca ctggggcac actcggagc ggtggcatc 
      ggagcggcg ggattggat gggttgggg gcgctgtcg 
      tcggatgaa gacactatg ccgcagctt tcacccgaa 
      cctgaagaa ctcgaggat gaaggacag ttcgctttc 
      cgcaggaat aagctgtgc tcttatcac atgccgttg 
      cccacggag ggtaactgg ccgtggtgt tcgaaagaa 
      gagaacggc ttggcggac aagcgcttc cgcttcacg 
      ttgacgtct ctgtcgaac cctcgccgt tgcataggt 
      ttcgcccga agacgcatc ggtcgagga ggaagggtc 
      atcctggac cgcatcgga accgaccac gatgatttc 
      tggcgaacg ttggatttc acgatcgtc gaaccgaac 
      gctaaaaaa gaagaacaa caaaacgaa acgaatttg 
      tcgtcggac ggtgtagtt aaaatggac gctagtttt 
      gttaataat aatacgaac gtaaacaat aatttacga 
      actgatatt aaaagtgag tatggttat gtcagtaac 
      agtttttca cggacgaat gtgaatgat gtcggtggt 
      gagagtgaa agtgggggc caaatcgat gtcaaaaaa 
      gaggtcgaa gaggatgga ggaccacaa ccggaaact 
      tttgcacat agcaaaagt gacgaggac gaaatggtt 
      gaaattttg cggtctgtt cggagggat tggctgcac 
      ttccgaccg aagacgccg ccccccgac gaagagtcc 
      ccgtgcagt ttgctcccg ttcagcgag agttatttc 
      gtccccggt gccaacatg tcgttctca atcgaggtc 
      cagtcgttg gaaacgcca agtacactc ctggacact 
      tccgctttc ttaaccgat ccattcacg tttgataat 
      ttggacagt atagaagac gtaccggta accgacacg 
      cggcaatcc agtgataaa atcgtgcaa acgggcatt 
      aacttgaac gttaatgtc ggtctcaat acgcttact 
      cctagtgtt aacgataat agtagtagt gttttttca 
      aatagtaac gcgaacctc actgatagt atcaagctc 
      gaggatgcg acgtcatcg tcgacgtcg gacgaaaac 
      gacgaaagg actatcgga tgttcgagg ttttacgtg 
      tcggatttg ggcgtcacg aagaaaaat caaacgtcc 
      aaaacgcaa accgttacg agtaccttg actagtcct 
      aagtttaat agtgtcaac gccgtgtct gagggatgg 
      agtaggagt acagccacc gaagggccc ttgactaac 
      accaacgga ctgctctcg cagtttggc ctggatggt 
      aggacaaaa aacaataaa atatcttac gcttactca 
      ggcggagtt taccaaact gtaccaccc atcaatgct 
      tcctctggc acattgagt cttcacact tctgcacac 
      actcttacg cttaatagt catagtgcc aacatttta 
      gaaataccg ataaccgac gacacggaa cccgtctcg 
      aacaaactg gtgaattct caatgtaca gaaccaggc 
      acagctacg acaaacaac aaaacgaaa accatcgtc 
      cggaagaac aatctggta atggaagtg acgtga
    mRNA sequence
    • >TC008956 RA
      tgttggtag taaatttct atggttcca tgacagagc 
      cgccatgat ggctttctg tataacacg ctcaacatg 
      caaagtttc aaagttgtt ttactgtat ttgtgactg 
      agtgcgtgt aaacaaaaa caatgggaa cgtactaag 
      gaccgcgaa gcaccgttt agtgtccgt ctcggacgg 
      tccgagacg cgggaaggc cgttttcgg gcgcgtttt 
      atgttggcc ctgtcgtgt gttgtgtgc tgtcaacaa 
      atatgtcca agctttcgt tcagggcga gggccctgg 
      acgccagca agcccatgc ccatataca tggcggagg 
      agctcccgg acctcccgg actactcgg cgatcaacc 
      gagcggtcc cccagatgc cctcgggca tggaaaagg 
      aggaggaat gcgagcacc atctgcaaa gggcgatat 
      gtgcggggc tcatcatcc ccacaccgg aagtttcgg 
      acatgccag acaaggaat tctacgaca aggtttatc 
      ccgccaact ataaacagc cccggcagc tcatccaca 
      tgcaaccct tcacgatgg aacaagaca tccctgact 
      acgacatgg actcggaag acgaacgtt ggttgcaat 
      cgcaggcgc agaggctgg atctgactc ctctaaagt 
      tcgaggaga tgatggacc gtttggaga agagcagcg 
      gccagactg tggtgaatc tgaacgagg ccaaagctc 
      tgctgaagg aggatgacg atctcataa tcgcagtat 
      tcgattatt ggttaaaca aacgcctga aaacccaac 
      atcccctaa tcctgacgg tgaagacgg aacagagag 
      ccggcgccg cccccaaca acccgtacc tggcgttcc 
      gacggcgaa cggagaaga tgcagaccc gcaagaacc 
      gcaaaaacg acgaaacat cgtacgaaa aaatgctaa 
      aacttcgta gggatctcc atcgggccg tcacgttgc 
      tcgaactcg tcaagaaac gggagaaga tgaagcggg 
      aacacgtct atctcacag tggaaacgt tcgagaaga 
      gattccatg cgagggact tcagtggtg ccatctatg 
      cggaagcag cagcgatca agacgacga ggccggcct 
      tcacaccga tctttcaca atcactatc cgaaccaga 
      gctggtcga ataaggcga tactcaaag acgaagtta 
      tacccagga aagagaaaa gacagtata aaaagagga 
      aacacaaat cactggggc acactcgga gcggtggca 
      tcggagcgg cgggattgg atgggttgg gggcgctgt 
      cgtcggatg aagacacta tgccgcagc tttcacccg 
      aacctgaag aactcgagg atgaaggac agttcgctt 
      tccgcagga ataagctgt gctcttatc acatgccgt 
      tgcccacgg agggtaact ggccgtggt gttcgaaag 
      aagagaacg gcttggcgg acaagcgct tccgcttca 
      cgttgacgt ctctgtcga accctcgcc gttgcatag 
      gtttcgccc gaagacgca tcggtcgag gaggaaggg 
      tcatcctgg accgcatcg gaaccgacc acgatgatt 
      tctggcgaa cgttggatt tcacgatcg tcgaaccga 
      acgctaaaa aagaagaac aacaaaacg aaacgaatt 
      tgtcgtcgg acggtgtag ttaaaatgg acgctagtt 
      ttgttaata ataatacga acgtaaaca ataatttac 
      gaactgata ttaaaagtg agtatggtt atgtcagta 
      acagttttt cacggacga atgtgaatg atgtcggtg 
      gtgagagtg aaagtgggg gccaaatcg atgtcaaaa 
      aagaggtcg aagaggatg gaggaccac aaccggaaa 
      cttttgcac atagcaaaa gtgacgagg acgaaatgg 
      ttgaaattt tgcggtctg ttcggaggg attggctgc 
      acttccgac cgaagacgc cgccccccg acgaagagt 
      ccccgtgca gtttgctcc cgttcagcg agagttatt 
      tcgtccccg gtgccaaca tgtcgttct caatcgagg 
      tccagtcgt tggaaacgc caagtacac tcctggaca 
      cttccgctt tcttaaccg atccattca cgtttgata 
      atttggaca gtatagaag acgtaccgg taaccgaca 
      cgcggcaat ccagtgata aaatcgtgc aaacgggca 
      ttaacttga acgttaatg tcggtctca atacgctta 
      ctcctagtg ttaacgata atagtagta gtgtttttt 
      caaatagta acgcgaacc tcactgata gtatcaagc 
      tcgaggatg cgacgtcat cgtcgacgt cggacgaaa 
      acgacgaaa ggactatcg gatgttcga ggttttacg 
      tgtcggatt tgggcgtca cgaagaaaa atcaaacgt 
      ccaaaacgc aaaccgtta cgagtacct tgactagtc 
      ctaagttta atagtgtca acgccgtgt ctgagggat 
      ggagtagga gtacagcca ccgaagggc ccttgacta 
      acaccaacg gactgctct cgcagtttg gcctggatg 
      gtaggacaa aaaacaata aaatatctt acgcttact 
      caggcggag tttaccaaa ctgtaccac ccatcaatg 
      cttcctctg gcacattga gtcttcaca cttctgcac 
      acactctta cgcttaata gtcatagtg ccaacattt 
      tagaaatac cgataaccg acgacacgg aacccgtct 
      cgaacaaac tggtgaatt ctcaatgta cagaaccag 
      gcacagcta cgacaaaca acaaaacga aaaccatcg 
      tccggaaga acaatctgg taatggaag tgacgtgat 
      gctcatctt cagacctgc gtttgtcga aagcgcagg 
      atggctttt tactatcaa caaggtgtt tcattttct 
      gctggttat acgttttca agatttgaa atgcctgtc 
      agaattttt acgttctcg tcattcaga ttgtacaca 
      agtattagt aagaagaaa aaaagttgc aaagattat 
      gggaaaagt gagcagggc gtttatttc gtaatcgat 
      ttgtttatc aagccgaga acgcggcgc tttatggga 
      tataatggt gttattttt cctcgatcg attttattc 
      agtacctac gattacaca taagttgaa ttttaatgg 
      cttctcaga ttttagatg tgcataatt gaatacatt 
      tgtttaaag tgtagacag tcgtgctgg agtaacatc 
      gttatattt catagtcta ttgttcatc tgtcgtgct 
      gtttgtata catttcgta tgaatgtat taatttcag 
      tgtataccg cataatctc cagaactgt tgcataata 
      ttttaattt tttaagacg aacatattc taactttat 
      agtattatt gtgttcatt aagtgatca taaattact 
      cagttttag ttgaataaa tttgtagag aaagcaggc 
      attttcgca aaatcttac caataaact agacttcta 
      ttttaagga gttgttaat ttattttct attgtaatt 
      ataacccta gtgttaata attcatgag gctgttgaa 
      ctattatta gtaacttat tacattttg taaatgaaa 
      ttttgtgaa atgtactta caccattag accactgta 
      ttttaaagc gattaaatt ttttagcca tcatttcca 
      cattttgtg ttcttaata catatgtgc aagcggaac 
      gaaaacaag ttaacaaat gtaagaagt tagtctgag 
      tctcgtgaa cttctatct gtataaaat attggcacg 
      ttatgagtt taaatagtt aacaatttg tcaaaagtg 
      gtgttacgt ctttcaata taagcttct ctgtgtcta 
      tttaactat tacaatcct acattggca gatcgttaa 
      cggtataaa acgttttta aaatttaat ttgaaaaca 
      agtttattt cattgtatg ttttataaa caaactaaa 
      aaggctgtt agaaaattg gtataaata cgctctctt 
      aatttctta taagtaaat tcgaaaatt tttactatt 
      ttgaataac acttttgct cgtttatta aaaactatt 
      gtaaataat acttttgtt tgtatataa ttatcaaga 
      ctagcagta ttttttgta caaaagttt gtcaattat 
      gttttaggt gttaagtat cataattta gggcaaagg 
      tattattta aaacaagtt taaatttaa aattagaat 
      caatggtgc aagctatta gataataag tcacattcc 
      tacttctgt atgtcattg tttgaatta acggtttaa 
      atgcatcca tgtgattgt cgattttct tatgttaaa 
      atttagtaa tttgatatt tagactgtt aattcatta 
      atttattat aaacgaaaa aaataataa agagccgga 
      cattcaaca atgtagaac ttgtctatg ttgttttat 
      gttgagaaa atcccaaaa aaatataat tgcttcaac 
      attgattgt ggcacttat tacaactaa aattttagc 
      attcacact ataaaaaac tgaggtttt cttttaccg 
      taatgtgaa tgttcgaat ttttagtgc ggataagtt 
      gtagaatgt ggtatttaa tttataata tacataaaa 
      tggaaaaaa attaataac gcgattact atatacggg 
      tctattaat gaacgttct gggtttttg attaggtcc 
      tctgaatgt gaatatttg taaatatgt gattagttt 
      atgtgtatg aatgatcaa taaaatctg tcatgcagg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY