Status
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Gene information
General information
TC009861 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 12966414..12973809
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009861
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02859

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     larva bristle shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    antenna antennal flagellum shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009861 RA
      MATPRSALK HGLFLFLAF IFVEGVDDY SNSFISPNS 
ARDSHSKIE EIKSGARTS APDIILVTS STISSHSHK 
FKRSPDPIL SRTDVDSFL TYKNVGESL DNNDVELSG 
RVLNINPSK TDHQQARSE VEQTVLSGE GPGAPQGRS 
LKEPQEISP TATAPATTA TALPKGRQA NPDIQDIIT 
GIVKLLNGN VNVQANTAP VMGRPLRPL STRINNRGP 
PRITDVPAL PPDFDIPAP PLPPPPLGQ KPPPVTSTR 
IPTPYPFDI PPHNTSPVK RPGLYRPVT IPPWNRRKP 
PSRRPIPPY KPMPPINSD TISLTTEKP IQDILTLDL 
GSQLQPTEE ELNYDGYEE NKSNQTFTN SNESSAVQS 
NDKEITDFE KNKEKSTSK MNKHAASTT KTTTTVKSQ 
TTTPTSTST STAASTTED QISSTSTST STLSSTLSS 
ISPTSTATI EPTSVLSST STQTTSLAL STNITNSTM 
SFIKSEVTP VLESSIQEV LQTLKDDLL TSTTLASTP 
TEQLPKISS SKPDKTVTE ASLQTNLAS ASDNKTSSQ 
GFPYYPYRP RPGIVLDDT EYKPGTHRQ PILTRPPIG 
QLGDIFDIT VSAIQGPGG STSDQGKPY IISVDLENV 
HHLGQGGDV ITSPVGDEG FVSIDGKRT YLNLFGDGS 
VSLTSAAPV IKPSAPPSN QVTGTGYAV VDEKPKPNL 
GQVKPTQRR PVFGRPRPS QPPVRIDTC IVGDDSTCD 
GSQHEVCKT EAGVSACHC RPGHARRKH REPCRKIVS 
VLLSLRVDR LYERRIVWA NELADKSST SYQQLAYEA 
ERALDSAMS MTPFSDEFL GAKVNSIYR GERSAGQPG 
VFVNLTLQL DENAETARS SVRGDIQKH LLGVIQRRS 
NNVGNSALW VDSPPGSIS NLQDLDECS SPELHDCHA 
LAKCINIFG GFRCECGDG YRDIWADNK HRAGRHCEQ 
CSAQHCNNR GECKYQNGL EVCICSGNY YGNQCELDG 
EVLGVAIGA SVAAVIIIG LTLVCLVMW SRRWSREQK 
AAVGSPVFG YMATASNTV KAPVVGAPP YQLTLEDRL 
RWAQIADVM AQANHYAPE PVTGPTRPS SAMFGYPNL 
SGTLPHGSM HGTLPPVPL PRLNLQAQM ASRAASVHG 
MRPLDNSSS SEEEDKADL LGRNFQVPR PKSRSNASI 
ANQSGIYYD VDYDQNEIY KQSGGIPLS TYSMGRPPY 
YRN
    CDS sequence
    • >TC009861 RA
      atggctaca cctaggtct gcactgaag catgggctt 
ttcctcttc ctggctttc atcttcgtt gaaggagtg 
gacgattac tccaactcg ttcatctcc ccgaactcc 
gctcgagac tcacactca aaaattgag gaaattaaa 
tcgggcgct cgcacttcc gcccctgat atcattttg 
gtcacttca tcgacgata tcttcgcac tcgcacaaa 
ttcaagcgg tcgcccgac cctatcttg tcacgtacg 
gacgttgac agcttcctc acgtacaaa aacgtgggg 
gagtccctg gacaacaac gacgtggag ttgtccggc 
cgcgtcctc aacataaat ccctccaag accgaccac 
cagcaggcc cgcagcgag gtggagcag accgtcctc 
tcgggggaa ggcccgggg gctcctcaa ggccggtcc 
ttgaaggag ccccaggag atctcgccg actgccacg 
gccccggcg actactgct accgccctc ccgaaagga 
cggcaagcc aaccctgac atccaggac atcatcacc 
ggaatcgtg aagctgctc aacggcaat gtcaacgtg 
caggcaaac acggctccg gtaatgggg cggcctctg 
cggccgctg tcaacacgg ataaataac aggggccct 
ccgaggatt acggacgtg cccgctttg ccgcccgat 
ttcgacatc cccgcgccg ccgctgccg ccgccgccg 
cttggccaa aaaccgccg ccagtcact tcgacaaga 
attcccacg ccttatcct tttgatatc ccgccgcat 
aatacttcg ccggttaag aggccgggg ctttatagg 
cccgttacg attccgccc tggaaccgc agaaaaccg 
ccaagtcgg agacccatc ccgccgtac aagcctatg 
ccgccgatt aactccgac actatcagt ttgacgact 
gaaaaaccc attcaggac attttaacg ctggatttg 
ggctcgcag ctgcagccc accgaagag gaacttaat 
tacgacggt tatgaggaa aataaaagc aatcagact 
tttactaac tcgaatgaa agtagtgcg gtgcaatct 
aacgataag gaaatcacc gactttgaa aagaataaa 
gagaaaagc acgtcaaag atgaacaaa catgcggct 
tcaactacg aaaaccacg acaactgtt aaatcacaa 
actacaacc cctacttcg acttcgacc tcaacggct 
gcttcaaca accgaagac caaatatct tcaacgtcc 
acctcaact tccaccttg tcgtcaaca ctatccagc 
atttctcca actagtaca gcaactata gaaccaacg 
agtgttctt tctagtacc agtactcaa actacgagt 
ttagccttg tcaacaaat atcacaaat agcacaatg 
tctttcatt aaaagtgaa gtgacccca gtgttagaa 
tcctcaatt caagaagtt ctacaaact ctgaaggac 
gatttgttg acttctacc actctagca agcacacca 
accgaacag ttgcccaaa atttcgtcc tccaaacct 
gataaaacc gtaacagaa gctagcttg caaactaat 
ttagcatca gcttcggat aataaaaca tccagccaa 
ggttttccc tattacccc taccgcccg cgcccgggc 
atagtcctc gacgacact gaatacaag cccgggacc 
caccgccag cccatcctg acccgtcca cccatcggc 
cagctgggc gacatcttc gacataacc gtctccgcc 
atccaaggc cctggcggc tcgacttcc gaccaaggc 
aaaccctac atcatctca gtcgactta gaaaacgtg 
catcattta ggccagggc ggtgatgtc atcaccagc 
cccgtgggc gacgagggc ttcgtttcc atcgacggc 
aaacgcacc tacttgaac ctcttcggc gacggaagt 
gtgtcgcta acttcggca gcccccgtc atcaagccc 
agcgcaccg cccagcaac caagtcacc ggaacgggc 
tatgccgtg gtcgacgaa aaacccaaa cccaacctg 
ggacaagtg aaaccgacc cagaggagg cccgttttt 
ggccgacca cgacctagc cagccacct gtgcgcatt 
gatacgtgc atagtgggt gatgactcg acctgtgat 
gggtcccaa cacgaggtg tgcaagacg gaggctggg 
gtcagcgcc tgtcattgt cggccagga catgccagg 
cggaagcac cgagagccg tgcaggaaa atcgtctcg 
gtgcttctg tctctgaga gttgaccgc ttgtacgaa 
aggagaatt gtttgggcc aacgaactg gccgataag 
tccagcacg agttatcaa caactggcc tacgaggcc 
gaaagagct ttggattcc gccatgtcg atgacgcct 
ttcagcgac gagtttctg ggtgctaaa gtgaacagt 
atctatcga ggtgaaagg tcagcaggt cagcctgga 
gtctttgtc aacttgact ctccaactc gacgaaaac 
gccgaaact gccagatct tcagtccgg ggagacatc 
caaaaacac ttattagga gtcatccaa cggagaagt 
aacaatgtt ggtaacagc gcattatgg gttgatagt 
cctccaggt tccatctcc aatttgcaa gacttggac 
gagtgttcc tcacccgaa ctgcacgac tgtcacgct 
ttggccaaa tgcatcaac atttttggg ggctttaga 
tgcgaatgt ggtgacggg taccgagac atttgggcc 
gacaacaag cacagagct gggagacac tgtgagcag 
tgctctgcc caacactgt aacaacaga ggggagtgt 
aaataccaa aacggacta gaagtttgc atatgttcc 
ggcaactac tacgggaac cagtgcgag ctggacggc 
gaggttctt ggagtggcg atcggggcc agtgtagca 
gccgtgatc ataatcggg ctgactctc gtctgtttg 
gtcatgtgg agccgtcgt tggagccgc gagcagaaa 
gcggccgtt ggcagcccc gtgttcggc tacatggcc 
actgcgagt aacacagtc aaggctccg gtcgtggga 
gcgcctccg tatcaactg accctagaa gatagactg 
agatgggct caaatcgcc gatgttatg gcccaagcc 
aatcattac gctccggaa ccggtaact ggaccaaca 
agaccgtca agtgccatg tttggttat cctaattta 
agcggtact cttccgcat ggttcaatg catggaact 
ctaccacct gtgcctctg ccacggctt aatttgcaa 
gcgcaaatg gcaagtcga gctgccagt gttcatggg 
atgcggccg ttggacaat tccagttcg agtgaagag 
gaagacaaa gcggacttg ctagggcgg aacttccaa 
gtgccgagg cccaaaagc aggagcaat gcatcgatt 
gctaatcaa agtggtatt tattatgac gtagactac 
gaccagaat gagatatac aaacagtcc gggggaatc 
cccttgagt acctacagc atggggcgc cctccctat 
tacaggaat taa
    mRNA sequence
    • >TC009861 RA
      gtaaaaccg agccacata cctcctgaa caaggtagg 
aacacactc aagtgttct tcaccaacg cgtcccgtt 
atttcacca gtcttctga agaaacgtt gtgcatcgt 
catcaaacg ctgttgcga aataattcc acgggaaat 
aggctcaat ggggcgctt gggccaaaa gtgacccat 
caaagggtg aaaacagtg ccttatcgg gaaaagaaa 
gttggtgct ttgatgagt gttatggtg gttttgaac 
aattacatc tccggatga cctaaatag ccgtttctg 
acaaacacg accgtgtct cacggccgg tttcattgt 
tgttcctta agacaccat taacataac gtgtgtgtt 
cgttcggtc accggagat gacccccgg agtgagtta 
tcgcgcttt ctgctgcgg gggctgctg attagaccg 
gctaaatgt ggtgtgatg tgattgaac tgtgacaaa 
atggctaca cctaggtct gcactgaag catgggctt 
ttcctcttc ctggctttc atcttcgtt gaaggagtg 
gacgattac tccaactcg ttcatctcc ccgaactcc 
gctcgagac tcacactca aaaattgag gaaattaaa 
tcgggcgct cgcacttcc gcccctgat atcattttg 
gtcacttca tcgacgata tcttcgcac tcgcacaaa 
ttcaagcgg tcgcccgac cctatcttg tcacgtacg 
gacgttgac agcttcctc acgtacaaa aacgtgggg 
gagtccctg gacaacaac gacgtggag ttgtccggc 
cgcgtcctc aacataaat ccctccaag accgaccac 
cagcaggcc cgcagcgag gtggagcag accgtcctc 
tcgggggaa ggcccgggg gctcctcaa ggccggtcc 
ttgaaggag ccccaggag atctcgccg actgccacg 
gccccggcg actactgct accgccctc ccgaaagga 
cggcaagcc aaccctgac atccaggac atcatcacc 
ggaatcgtg aagctgctc aacggcaat gtcaacgtg 
caggcaaac acggctccg gtaatgggg cggcctctg 
cggccgctg tcaacacgg ataaataac aggggccct 
ccgaggatt acggacgtg cccgctttg ccgcccgat 
ttcgacatc cccgcgccg ccgctgccg ccgccgccg 
cttggccaa aaaccgccg ccagtcact tcgacaaga 
attcccacg ccttatcct tttgatatc ccgccgcat 
aatacttcg ccggttaag aggccgggg ctttatagg 
cccgttacg attccgccc tggaaccgc agaaaaccg 
ccaagtcgg agacccatc ccgccgtac aagcctatg 
ccgccgatt aactccgac actatcagt ttgacgact 
gaaaaaccc attcaggac attttaacg ctggatttg 
ggctcgcag ctgcagccc accgaagag gaacttaat 
tacgacggt tatgaggaa aataaaagc aatcagact 
tttactaac tcgaatgaa agtagtgcg gtgcaatct 
aacgataag gaaatcacc gactttgaa aagaataaa 
gagaaaagc acgtcaaag atgaacaaa catgcggct 
tcaactacg aaaaccacg acaactgtt aaatcacaa 
actacaacc cctacttcg acttcgacc tcaacggct 
gcttcaaca accgaagac caaatatct tcaacgtcc 
acctcaact tccaccttg tcgtcaaca ctatccagc 
atttctcca actagtaca gcaactata gaaccaacg 
agtgttctt tctagtacc agtactcaa actacgagt 
ttagccttg tcaacaaat atcacaaat agcacaatg 
tctttcatt aaaagtgaa gtgacccca gtgttagaa 
tcctcaatt caagaagtt ctacaaact ctgaaggac 
gatttgttg acttctacc actctagca agcacacca 
accgaacag ttgcccaaa atttcgtcc tccaaacct 
gataaaacc gtaacagaa gctagcttg caaactaat 
ttagcatca gcttcggat aataaaaca tccagccaa 
ggttttccc tattacccc taccgcccg cgcccgggc 
atagtcctc gacgacact gaatacaag cccgggacc 
caccgccag cccatcctg acccgtcca cccatcggc 
cagctgggc gacatcttc gacataacc gtctccgcc 
atccaaggc cctggcggc tcgacttcc gaccaaggc 
aaaccctac atcatctca gtcgactta gaaaacgtg 
catcattta ggccagggc ggtgatgtc atcaccagc 
cccgtgggc gacgagggc ttcgtttcc atcgacggc 
aaacgcacc tacttgaac ctcttcggc gacggaagt 
gtgtcgcta acttcggca gcccccgtc atcaagccc 
agcgcaccg cccagcaac caagtcacc ggaacgggc 
tatgccgtg gtcgacgaa aaacccaaa cccaacctg 
ggacaagtg aaaccgacc cagaggagg cccgttttt 
ggccgacca cgacctagc cagccacct gtgcgcatt 
gatacgtgc atagtgggt gatgactcg acctgtgat 
gggtcccaa cacgaggtg tgcaagacg gaggctggg 
gtcagcgcc tgtcattgt cggccagga catgccagg 
cggaagcac cgagagccg tgcaggaaa atcgtctcg 
gtgcttctg tctctgaga gttgaccgc ttgtacgaa 
aggagaatt gtttgggcc aacgaactg gccgataag 
tccagcacg agttatcaa caactggcc tacgaggcc 
gaaagagct ttggattcc gccatgtcg atgacgcct 
ttcagcgac gagtttctg ggtgctaaa gtgaacagt 
atctatcga ggtgaaagg tcagcaggt cagcctgga 
gtctttgtc aacttgact ctccaactc gacgaaaac 
gccgaaact gccagatct tcagtccgg ggagacatc 
caaaaacac ttattagga gtcatccaa cggagaagt 
aacaatgtt ggtaacagc gcattatgg gttgatagt 
cctccaggt tccatctcc aatttgcaa gacttggac 
gagtgttcc tcacccgaa ctgcacgac tgtcacgct 
ttggccaaa tgcatcaac atttttggg ggctttaga 
tgcgaatgt ggtgacggg taccgagac atttgggcc 
gacaacaag cacagagct gggagacac tgtgagcag 
tgctctgcc caacactgt aacaacaga ggggagtgt 
aaataccaa aacggacta gaagtttgc atatgttcc 
ggcaactac tacgggaac cagtgcgag ctggacggc 
gaggttctt ggagtggcg atcggggcc agtgtagca 
gccgtgatc ataatcggg ctgactctc gtctgtttg 
gtcatgtgg agccgtcgt tggagccgc gagcagaaa 
gcggccgtt ggcagcccc gtgttcggc tacatggcc 
actgcgagt aacacagtc aaggctccg gtcgtggga 
gcgcctccg tatcaactg accctagaa gatagactg 
agatgggct caaatcgcc gatgttatg gcccaagcc 
aatcattac gctccggaa ccggtaact ggaccaaca 
agaccgtca agtgccatg tttggttat cctaattta 
agcggtact cttccgcat ggttcaatg catggaact 
ctaccacct gtgcctctg ccacggctt aatttgcaa 
gcgcaaatg gcaagtcga gctgccagt gttcatggg 
atgcggccg ttggacaat tccagttcg agtgaagag 
gaagacaaa gcggacttg ctagggcgg aacttccaa 
gtgccgagg cccaaaagc aggagcaat gcatcgatt 
gctaatcaa agtggtatt tattatgac gtagactac 
gaccagaat gagatatac aaacagtcc gggggaatc 
cccttgagt acctacagc atggggcgc cctccctat 
tacaggaat taaacgttg ttttgtaaa tatttaaca 
taaaacact aaaaacaga tatttatgg aatgaataa 
aagactatg gatagtatt attttagta gaatatttt 
acattgtta taatttgta ttttactgt cagtaagcg 
aataaaagg caattaatt agcgggtag ttgttatgt 
atccaactg tgtaatttc ttgtaaata tcaaaatcg 
attgagttt ggccgtttt gtattttgt gtaattaat 
aaaaagctc caaacaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY