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Gene information
General information
TC009861 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 12966414..12973809
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009861
    iBeetle screen iB_02859

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva bristle shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    antenna antennal flagellum shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009861 RA
      MATPRSALK HGLFLFLAF IFVEGVDDY SNSFISPNS 
      ARDSHSKIE EIKSGARTS APDIILVTS STISSHSHK 
      FKRSPDPIL SRTDVDSFL TYKNVGESL DNNDVELSG 
      RVLNINPSK TDHQQARSE VEQTVLSGE GPGAPQGRS 
      LKEPQEISP TATAPATTA TALPKGRQA NPDIQDIIT 
      GIVKLLNGN VNVQANTAP VMGRPLRPL STRINNRGP 
      PRITDVPAL PPDFDIPAP PLPPPPLGQ KPPPVTSTR 
      IPTPYPFDI PPHNTSPVK RPGLYRPVT IPPWNRRKP 
      PSRRPIPPY KPMPPINSD TISLTTEKP IQDILTLDL 
      GSQLQPTEE ELNYDGYEE NKSNQTFTN SNESSAVQS 
      NDKEITDFE KNKEKSTSK MNKHAASTT KTTTTVKSQ 
      TTTPTSTST STAASTTED QISSTSTST STLSSTLSS 
      ISPTSTATI EPTSVLSST STQTTSLAL STNITNSTM 
      SFIKSEVTP VLESSIQEV LQTLKDDLL TSTTLASTP 
      TEQLPKISS SKPDKTVTE ASLQTNLAS ASDNKTSSQ 
      GFPYYPYRP RPGIVLDDT EYKPGTHRQ PILTRPPIG 
      QLGDIFDIT VSAIQGPGG STSDQGKPY IISVDLENV 
      HHLGQGGDV ITSPVGDEG FVSIDGKRT YLNLFGDGS 
      VSLTSAAPV IKPSAPPSN QVTGTGYAV VDEKPKPNL 
      GQVKPTQRR PVFGRPRPS QPPVRIDTC IVGDDSTCD 
      GSQHEVCKT EAGVSACHC RPGHARRKH REPCRKIVS 
      VLLSLRVDR LYERRIVWA NELADKSST SYQQLAYEA 
      ERALDSAMS MTPFSDEFL GAKVNSIYR GERSAGQPG 
      VFVNLTLQL DENAETARS SVRGDIQKH LLGVIQRRS 
      NNVGNSALW VDSPPGSIS NLQDLDECS SPELHDCHA 
      LAKCINIFG GFRCECGDG YRDIWADNK HRAGRHCEQ 
      CSAQHCNNR GECKYQNGL EVCICSGNY YGNQCELDG 
      EVLGVAIGA SVAAVIIIG LTLVCLVMW SRRWSREQK 
      AAVGSPVFG YMATASNTV KAPVVGAPP YQLTLEDRL 
      RWAQIADVM AQANHYAPE PVTGPTRPS SAMFGYPNL 
      SGTLPHGSM HGTLPPVPL PRLNLQAQM ASRAASVHG 
      MRPLDNSSS SEEEDKADL LGRNFQVPR PKSRSNASI 
      ANQSGIYYD VDYDQNEIY KQSGGIPLS TYSMGRPPY 
      YRN
    CDS sequence
    • >TC009861 RA
      atggctaca cctaggtct gcactgaag catgggctt 
      ttcctcttc ctggctttc atcttcgtt gaaggagtg 
      gacgattac tccaactcg ttcatctcc ccgaactcc 
      gctcgagac tcacactca aaaattgag gaaattaaa 
      tcgggcgct cgcacttcc gcccctgat atcattttg 
      gtcacttca tcgacgata tcttcgcac tcgcacaaa 
      ttcaagcgg tcgcccgac cctatcttg tcacgtacg 
      gacgttgac agcttcctc acgtacaaa aacgtgggg 
      gagtccctg gacaacaac gacgtggag ttgtccggc 
      cgcgtcctc aacataaat ccctccaag accgaccac 
      cagcaggcc cgcagcgag gtggagcag accgtcctc 
      tcgggggaa ggcccgggg gctcctcaa ggccggtcc 
      ttgaaggag ccccaggag atctcgccg actgccacg 
      gccccggcg actactgct accgccctc ccgaaagga 
      cggcaagcc aaccctgac atccaggac atcatcacc 
      ggaatcgtg aagctgctc aacggcaat gtcaacgtg 
      caggcaaac acggctccg gtaatgggg cggcctctg 
      cggccgctg tcaacacgg ataaataac aggggccct 
      ccgaggatt acggacgtg cccgctttg ccgcccgat 
      ttcgacatc cccgcgccg ccgctgccg ccgccgccg 
      cttggccaa aaaccgccg ccagtcact tcgacaaga 
      attcccacg ccttatcct tttgatatc ccgccgcat 
      aatacttcg ccggttaag aggccgggg ctttatagg 
      cccgttacg attccgccc tggaaccgc agaaaaccg 
      ccaagtcgg agacccatc ccgccgtac aagcctatg 
      ccgccgatt aactccgac actatcagt ttgacgact 
      gaaaaaccc attcaggac attttaacg ctggatttg 
      ggctcgcag ctgcagccc accgaagag gaacttaat 
      tacgacggt tatgaggaa aataaaagc aatcagact 
      tttactaac tcgaatgaa agtagtgcg gtgcaatct 
      aacgataag gaaatcacc gactttgaa aagaataaa 
      gagaaaagc acgtcaaag atgaacaaa catgcggct 
      tcaactacg aaaaccacg acaactgtt aaatcacaa 
      actacaacc cctacttcg acttcgacc tcaacggct 
      gcttcaaca accgaagac caaatatct tcaacgtcc 
      acctcaact tccaccttg tcgtcaaca ctatccagc 
      atttctcca actagtaca gcaactata gaaccaacg 
      agtgttctt tctagtacc agtactcaa actacgagt 
      ttagccttg tcaacaaat atcacaaat agcacaatg 
      tctttcatt aaaagtgaa gtgacccca gtgttagaa 
      tcctcaatt caagaagtt ctacaaact ctgaaggac 
      gatttgttg acttctacc actctagca agcacacca 
      accgaacag ttgcccaaa atttcgtcc tccaaacct 
      gataaaacc gtaacagaa gctagcttg caaactaat 
      ttagcatca gcttcggat aataaaaca tccagccaa 
      ggttttccc tattacccc taccgcccg cgcccgggc 
      atagtcctc gacgacact gaatacaag cccgggacc 
      caccgccag cccatcctg acccgtcca cccatcggc 
      cagctgggc gacatcttc gacataacc gtctccgcc 
      atccaaggc cctggcggc tcgacttcc gaccaaggc 
      aaaccctac atcatctca gtcgactta gaaaacgtg 
      catcattta ggccagggc ggtgatgtc atcaccagc 
      cccgtgggc gacgagggc ttcgtttcc atcgacggc 
      aaacgcacc tacttgaac ctcttcggc gacggaagt 
      gtgtcgcta acttcggca gcccccgtc atcaagccc 
      agcgcaccg cccagcaac caagtcacc ggaacgggc 
      tatgccgtg gtcgacgaa aaacccaaa cccaacctg 
      ggacaagtg aaaccgacc cagaggagg cccgttttt 
      ggccgacca cgacctagc cagccacct gtgcgcatt 
      gatacgtgc atagtgggt gatgactcg acctgtgat 
      gggtcccaa cacgaggtg tgcaagacg gaggctggg 
      gtcagcgcc tgtcattgt cggccagga catgccagg 
      cggaagcac cgagagccg tgcaggaaa atcgtctcg 
      gtgcttctg tctctgaga gttgaccgc ttgtacgaa 
      aggagaatt gtttgggcc aacgaactg gccgataag 
      tccagcacg agttatcaa caactggcc tacgaggcc 
      gaaagagct ttggattcc gccatgtcg atgacgcct 
      ttcagcgac gagtttctg ggtgctaaa gtgaacagt 
      atctatcga ggtgaaagg tcagcaggt cagcctgga 
      gtctttgtc aacttgact ctccaactc gacgaaaac 
      gccgaaact gccagatct tcagtccgg ggagacatc 
      caaaaacac ttattagga gtcatccaa cggagaagt 
      aacaatgtt ggtaacagc gcattatgg gttgatagt 
      cctccaggt tccatctcc aatttgcaa gacttggac 
      gagtgttcc tcacccgaa ctgcacgac tgtcacgct 
      ttggccaaa tgcatcaac atttttggg ggctttaga 
      tgcgaatgt ggtgacggg taccgagac atttgggcc 
      gacaacaag cacagagct gggagacac tgtgagcag 
      tgctctgcc caacactgt aacaacaga ggggagtgt 
      aaataccaa aacggacta gaagtttgc atatgttcc 
      ggcaactac tacgggaac cagtgcgag ctggacggc 
      gaggttctt ggagtggcg atcggggcc agtgtagca 
      gccgtgatc ataatcggg ctgactctc gtctgtttg 
      gtcatgtgg agccgtcgt tggagccgc gagcagaaa 
      gcggccgtt ggcagcccc gtgttcggc tacatggcc 
      actgcgagt aacacagtc aaggctccg gtcgtggga 
      gcgcctccg tatcaactg accctagaa gatagactg 
      agatgggct caaatcgcc gatgttatg gcccaagcc 
      aatcattac gctccggaa ccggtaact ggaccaaca 
      agaccgtca agtgccatg tttggttat cctaattta 
      agcggtact cttccgcat ggttcaatg catggaact 
      ctaccacct gtgcctctg ccacggctt aatttgcaa 
      gcgcaaatg gcaagtcga gctgccagt gttcatggg 
      atgcggccg ttggacaat tccagttcg agtgaagag 
      gaagacaaa gcggacttg ctagggcgg aacttccaa 
      gtgccgagg cccaaaagc aggagcaat gcatcgatt 
      gctaatcaa agtggtatt tattatgac gtagactac 
      gaccagaat gagatatac aaacagtcc gggggaatc 
      cccttgagt acctacagc atggggcgc cctccctat 
      tacaggaat taa
    mRNA sequence
    • >TC009861 RA
      gtaaaaccg agccacata cctcctgaa caaggtagg 
      aacacactc aagtgttct tcaccaacg cgtcccgtt 
      atttcacca gtcttctga agaaacgtt gtgcatcgt 
      catcaaacg ctgttgcga aataattcc acgggaaat 
      aggctcaat ggggcgctt gggccaaaa gtgacccat 
      caaagggtg aaaacagtg ccttatcgg gaaaagaaa 
      gttggtgct ttgatgagt gttatggtg gttttgaac 
      aattacatc tccggatga cctaaatag ccgtttctg 
      acaaacacg accgtgtct cacggccgg tttcattgt 
      tgttcctta agacaccat taacataac gtgtgtgtt 
      cgttcggtc accggagat gacccccgg agtgagtta 
      tcgcgcttt ctgctgcgg gggctgctg attagaccg 
      gctaaatgt ggtgtgatg tgattgaac tgtgacaaa 
      atggctaca cctaggtct gcactgaag catgggctt 
      ttcctcttc ctggctttc atcttcgtt gaaggagtg 
      gacgattac tccaactcg ttcatctcc ccgaactcc 
      gctcgagac tcacactca aaaattgag gaaattaaa 
      tcgggcgct cgcacttcc gcccctgat atcattttg 
      gtcacttca tcgacgata tcttcgcac tcgcacaaa 
      ttcaagcgg tcgcccgac cctatcttg tcacgtacg 
      gacgttgac agcttcctc acgtacaaa aacgtgggg 
      gagtccctg gacaacaac gacgtggag ttgtccggc 
      cgcgtcctc aacataaat ccctccaag accgaccac 
      cagcaggcc cgcagcgag gtggagcag accgtcctc 
      tcgggggaa ggcccgggg gctcctcaa ggccggtcc 
      ttgaaggag ccccaggag atctcgccg actgccacg 
      gccccggcg actactgct accgccctc ccgaaagga 
      cggcaagcc aaccctgac atccaggac atcatcacc 
      ggaatcgtg aagctgctc aacggcaat gtcaacgtg 
      caggcaaac acggctccg gtaatgggg cggcctctg 
      cggccgctg tcaacacgg ataaataac aggggccct 
      ccgaggatt acggacgtg cccgctttg ccgcccgat 
      ttcgacatc cccgcgccg ccgctgccg ccgccgccg 
      cttggccaa aaaccgccg ccagtcact tcgacaaga 
      attcccacg ccttatcct tttgatatc ccgccgcat 
      aatacttcg ccggttaag aggccgggg ctttatagg 
      cccgttacg attccgccc tggaaccgc agaaaaccg 
      ccaagtcgg agacccatc ccgccgtac aagcctatg 
      ccgccgatt aactccgac actatcagt ttgacgact 
      gaaaaaccc attcaggac attttaacg ctggatttg 
      ggctcgcag ctgcagccc accgaagag gaacttaat 
      tacgacggt tatgaggaa aataaaagc aatcagact 
      tttactaac tcgaatgaa agtagtgcg gtgcaatct 
      aacgataag gaaatcacc gactttgaa aagaataaa 
      gagaaaagc acgtcaaag atgaacaaa catgcggct 
      tcaactacg aaaaccacg acaactgtt aaatcacaa 
      actacaacc cctacttcg acttcgacc tcaacggct 
      gcttcaaca accgaagac caaatatct tcaacgtcc 
      acctcaact tccaccttg tcgtcaaca ctatccagc 
      atttctcca actagtaca gcaactata gaaccaacg 
      agtgttctt tctagtacc agtactcaa actacgagt 
      ttagccttg tcaacaaat atcacaaat agcacaatg 
      tctttcatt aaaagtgaa gtgacccca gtgttagaa 
      tcctcaatt caagaagtt ctacaaact ctgaaggac 
      gatttgttg acttctacc actctagca agcacacca 
      accgaacag ttgcccaaa atttcgtcc tccaaacct 
      gataaaacc gtaacagaa gctagcttg caaactaat 
      ttagcatca gcttcggat aataaaaca tccagccaa 
      ggttttccc tattacccc taccgcccg cgcccgggc 
      atagtcctc gacgacact gaatacaag cccgggacc 
      caccgccag cccatcctg acccgtcca cccatcggc 
      cagctgggc gacatcttc gacataacc gtctccgcc 
      atccaaggc cctggcggc tcgacttcc gaccaaggc 
      aaaccctac atcatctca gtcgactta gaaaacgtg 
      catcattta ggccagggc ggtgatgtc atcaccagc 
      cccgtgggc gacgagggc ttcgtttcc atcgacggc 
      aaacgcacc tacttgaac ctcttcggc gacggaagt 
      gtgtcgcta acttcggca gcccccgtc atcaagccc 
      agcgcaccg cccagcaac caagtcacc ggaacgggc 
      tatgccgtg gtcgacgaa aaacccaaa cccaacctg 
      ggacaagtg aaaccgacc cagaggagg cccgttttt 
      ggccgacca cgacctagc cagccacct gtgcgcatt 
      gatacgtgc atagtgggt gatgactcg acctgtgat 
      gggtcccaa cacgaggtg tgcaagacg gaggctggg 
      gtcagcgcc tgtcattgt cggccagga catgccagg 
      cggaagcac cgagagccg tgcaggaaa atcgtctcg 
      gtgcttctg tctctgaga gttgaccgc ttgtacgaa 
      aggagaatt gtttgggcc aacgaactg gccgataag 
      tccagcacg agttatcaa caactggcc tacgaggcc 
      gaaagagct ttggattcc gccatgtcg atgacgcct 
      ttcagcgac gagtttctg ggtgctaaa gtgaacagt 
      atctatcga ggtgaaagg tcagcaggt cagcctgga 
      gtctttgtc aacttgact ctccaactc gacgaaaac 
      gccgaaact gccagatct tcagtccgg ggagacatc 
      caaaaacac ttattagga gtcatccaa cggagaagt 
      aacaatgtt ggtaacagc gcattatgg gttgatagt 
      cctccaggt tccatctcc aatttgcaa gacttggac 
      gagtgttcc tcacccgaa ctgcacgac tgtcacgct 
      ttggccaaa tgcatcaac atttttggg ggctttaga 
      tgcgaatgt ggtgacggg taccgagac atttgggcc 
      gacaacaag cacagagct gggagacac tgtgagcag 
      tgctctgcc caacactgt aacaacaga ggggagtgt 
      aaataccaa aacggacta gaagtttgc atatgttcc 
      ggcaactac tacgggaac cagtgcgag ctggacggc 
      gaggttctt ggagtggcg atcggggcc agtgtagca 
      gccgtgatc ataatcggg ctgactctc gtctgtttg 
      gtcatgtgg agccgtcgt tggagccgc gagcagaaa 
      gcggccgtt ggcagcccc gtgttcggc tacatggcc 
      actgcgagt aacacagtc aaggctccg gtcgtggga 
      gcgcctccg tatcaactg accctagaa gatagactg 
      agatgggct caaatcgcc gatgttatg gcccaagcc 
      aatcattac gctccggaa ccggtaact ggaccaaca 
      agaccgtca agtgccatg tttggttat cctaattta 
      agcggtact cttccgcat ggttcaatg catggaact 
      ctaccacct gtgcctctg ccacggctt aatttgcaa 
      gcgcaaatg gcaagtcga gctgccagt gttcatggg 
      atgcggccg ttggacaat tccagttcg agtgaagag 
      gaagacaaa gcggacttg ctagggcgg aacttccaa 
      gtgccgagg cccaaaagc aggagcaat gcatcgatt 
      gctaatcaa agtggtatt tattatgac gtagactac 
      gaccagaat gagatatac aaacagtcc gggggaatc 
      cccttgagt acctacagc atggggcgc cctccctat 
      tacaggaat taaacgttg ttttgtaaa tatttaaca 
      taaaacact aaaaacaga tatttatgg aatgaataa 
      aagactatg gatagtatt attttagta gaatatttt 
      acattgtta taatttgta ttttactgt cagtaagcg 
      aataaaagg caattaatt agcgggtag ttgttatgt 
      atccaactg tgtaatttc ttgtaaata tcaaaatcg 
      attgagttt ggccgtttt gtattttgt gtaattaat 
      aaaaagctc caaacaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY