| General information | |||
| TC009848 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 9042301..9064520 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009848 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02858 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Days post injection
Fertility
mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 -
Number of animals showing the phenotype: 2
mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) -
Penetrance (percentage of animals/eggs on slide): 50% - 80%
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
appendages random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
larva segment not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva constricted - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
legs shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
legs podomer potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
appendages random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
larva segment not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva constricted - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
legs shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
legs podomer potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
Pupal morphology
Adult morphology
- >TC009848 RA
MAEDPWLLN EDGYLNADT DIKEIIYHP TLNVILICT NSGIVRVLD VNSGVVLQS SYLSAINQN EVTCKYIPG QDRVLFCDG QAIGVRSDY NSVLLLDSI LQKPVSDPK KEIKLELPL SEAIILKQS LTSANVSGI EHVINELTD VILNAQKQS KKGIKAQKW NTVCLQLPL EMLQMAAMS SVSELLLKN QHTPELSVA SAVQERLSE LSGEQGSAS DRRSMASEA KRRETFTHW PHMDYKWAL PDQMAQAGF YHQPNASGD DRAMCFTCT VCLVCWERT DEPWSEHER HSPSCPFVM GEYTQNVPL SVTYATNPA IDATYRGVN VKILGTSTL SNLLPSANA EGLISVFDI SGKISRTHS FFVTQFDSH ILEKYTQDF GVPGTWSDL DEEKRITSL AIVSTAETT STRPTIICG LQIRCRAQK YQSPVIGSY DDQNKNLME VVESATSST SFSCLYLVV YDFMYSKEK EDKDHESKP NDVNAISEV TAPKNESTQ NENQKNSDY VHPDAVFVE HLSSFYKTL IPGESDEVF LPPPMLVNK PQHSGPRVL PIDMQITET DASQTEPFI INQEYSFMN GQLSSILSE TFEKSISDH ITEMKNNKT AKKLNYSRA VQCVSIPAP YKSRQDLEI SDIIATQDS RHVLVVLKS SLDGKSVLI LYSLDFSQK MVKLVEEPV LVRELNNNE KPTEVSMLP MVDKLGEFA EQGKNGVVG NIVMVCVDG AVRIVELST MKTICMAQL ESEKFVSAA YCNSLERLC ASTAKGSLH FYALNDTDN ESSEDHEED DLFGMTTDG TSSNPAQTS DSLDGSEMT RCFHDPPEI ISLIDLKRL HALCQFEPL RAGYCAVVP PCWSEIQQA QRQRRYPQS LKTDCDQYT KTWRLQTDT TTWDEHIFE ITLPSPMSL GHVDVHFSL QSSTALPHV EVTLLRQNT SGIGHKKDV KFAVDETIT IDMLQQWAD NPVISQEYL RAHNADILA GPVNIASCL DLSEQCGVV TLTSPKLFK SRNRTLLLH IRAVYSKEE ANNKTASKR KRSESKDKT GQCASHKNE FYMGCDCIH ELSITVYSS KHTEIPHER SQRNFMLES ISCVQSLLL TAMNNSSNE SVSLSLDVL TWIASIRLI RNRSNNGEV PNQQMEFLY VIEYNLAKL LQQCLLLGE RSIAHKCMK LIEVCCNGA KNMSETGRS HFEFSILKA LLSLLNTIT DVRSPGGLQ WIFALLLKV ISKNAQYVA TKCVTLLNK MSEELENRT NPYHLLLRS RYGLYGTPL ELELFDIEP PPYAKSTST YVTYATAVS GDSANQNND FFENYSFNK ESINPKDVL SGTETKLKW RNLAPPKIY RGLIETVPL HFTCVSASE GTRLERADA SNNINNIVI NEIPFSITP FHGQGSLDK MDHSKFKSY LVDFVEEPT VTVKEYKNS NPQATTSSV GTWDDNGCE VLIGELPVD VGNHLLKKL DSMYQDLKS SMENKSSAV KNVWQPESL LSPPTNSSL PWQHLLVTP PQQVIVVER MHSGARRYV VLDFGQPIL LTDILLPSC NDLVSVSID IWLKSEDVD GTRLVVAAD IGSKNLVLN DLQPAPLCR YMKITTVGR YGMTTARCR IPIGYFYGH VVVLPEEVP PDLSQNVSN LACTDLDKQ LTILSKLFE DISCRYSLA CSKLKELLR PFLVADMKN ASHLATYMN IMKDKALNT SNPEHSKIF SAYQESITY QRQLNTIRN VMNRLEGSI SKMSVSPRN PKDIPNAST DKLRAIAEG LLEVLLTLD SAIEIDPVT CRKLFQGLC VSQASRLQL LTAIFLDKS CRTKSYWGN FLADTLTEM FATSNTTKF PQDRVFILL AYLSRKSPE RSAVIDAGL RVVSQTLAP LTGDRSNLL AVSVDLPLL SWLLMYLSL QLDLSKNSL QSATRWDWV FGEIVGKVN AESAKIGSR KKNYKRISK QQIDNLDAA QKLVQSSAQ ALSTLSNHA ACLTSKLDA ALKTQQFLM KKVKYKFMD MKNSPAENP LAPKKLKET AAEPVIEKI TEKKMPQHI DVNHCLTVA KGLLTLVLS MDHSSSADM LLLSFKVMA RLVSMAKIK LGQLMNQEQ LLNLFRFCL ASKIPWAPH ALACLLQDV VENENDTDS GASTSWATN DFLSDVANL CEFTDDETF VHEPVITAP PKTGNNNNQ LPSVYESDD SDLEEIVDG FLDKEAMST PKKIPKPTN YSHTTNSVA LDSRLDLSV DVSAEISLR KLINKNTQG LLQSVNAPV VGDESEDAL APWPTVVLP LQPESLLSN TAMLTQCFD SLFENLPMQ DSANIEHIL QLWLTLNNP YKDDKFDPS AVAQIVLKE SSVKALISA IAWTPGLSL ITWCVALQT LTLVCNSNI TSESSLYWS DSYGMASCI VNHPDFVQV FMRLLSGTG LTFIDKVLA GPSLCKALN DFLDRLLMR CYVESPRSK AGKSLKTLL LKIVHQLVL PTGPIALRQ GPLDVQCKL LKIMLDIDY FNADMTIAM SIFESTCVL VHSYFVNVD KIKCINIGE KHNVVTTTF NEIFACVMG SDPIKMDLQ VSTEVLFLR LLKFLGKLV QTQFRQGID QPEAMDVEP LATSQTDES KAEQIQQDS MRNRGENFA DLVLQYHAN IMRLCQSLA ACKSSSLCM LANVSQKAA FSNLSEPSN VGDAVFHVL ALLAKRTSD KKLVLPPLL QFLAHAPQL SEPLLWFLL QILNNEEAI RNFLTAGGI EILGRSFVT SSNTPNTIS KSGTISIVM QHFSGINPT SDASTAIAS ASASRKLQQ ATIENKLSL VNFAPCCTI SCPSGTAQP AEVLIQGTA ATHRRARTP QWSYHFYPD EAHTELTLQ LPSAILLRE VHLQPHLSA LATCPSAVA LEVSANGPS RLVPVCPPL PTSGMVFIR LHLPVPEVV NCVLIRLYR PRDANNIGL SQIRLLGNL AFGGNTNQN LADPVEDES HCRHSLGWL RLLHHCFTL PTEQDLAKQ VIACASGVP RLLSTCCEL LLVPSHVLP IYLPCLEKV LRELSLHNL ENGNNVIKI LLNSRSSII EPLNFADNA WQDRLLINA TGYQSACEL LYQICEHQD SDTFYRVKM MLEWLQSVA VEAMQTGNT QGCNPAYIS SIASILWSA NQARVSYDL PSMITLDFF DTIYNLKTC VENNIALKY SLDSLLCSL CYIRPELFP VLLQRIGVL VPNLSTDHD ASISDDRKD SESMTDDNK QTFELDSEW YGHLVMGEL SKLSLSNEQ LETVALVSR SPSAIQQLL DSGLPKMLN SAIYEFCTS NEDNSVPMA KLENVTAIL KFFTDVSDE KTMRDWLGS SDGSSFWLE LLNWLCKKP FLKKSNLQS EAHAQLEEV CVKFLSKCC LCHPSNQAR LANVLCKVI DLQSNGISG FMRRLTLQL LLENEKIPV SIKADETLY KSVKLTQAY LPVHPAFKQ TYNRALLYL STNTTLADI LEQHIYFNT TYKMETNNS SKRPFVTKK DLFKGWFPE DSDLSMAAG VTAKDKRAK DAKNQAAST PQSKKKRYT ATESLTSGD VIGGRLIKC EAYSDQPLP LNLNLGQLL RLIESKGTT ADWPCVHLV VCQNKNGDK KSESSEVDS SLYQQQPFS SALQVFSSM GGLALLARH LPTVYPEAV RPPLKEKTT SEQSDSEWI KVEDCDDIY EDVEETVGT SSPSKSSGL ISNVPAHSL TAFGLFLRL PGYAEVLLK DMKKALCLL RLVLGVTDD GEGGDIFQS PIADSLPTL PFEVLRKLY DATPLSTDD GRLLRRISI NTGVVHLLL ACLGIFTHQ TQNNEKEGQ KDAKGKDER SQLYWAKGT GYGTGSTQQ IWNVEQALL KQKSEEEHV TVLLQVLAS YISPNGETA DELMEDVLP SSFHDLLAN SSLLPALSS YLRNDSVLD MARHIPLYR AALQLLRAL ATSSQLVDL LLPQKTRIN EPSISSLLK SMKTCVDTY ASKLRLNTK TNTKFKTKL REQLEELEQ GEGLATLMP DIQDTANLV SKVTSGLVD SDHDSEGEV AVDRVIFAS LEEKYLKIM KSLQFGSYE MITELPEGG VKFAVSHHF ENNAKSTGE QSHPARVKR IAQEAVTLS TSLPLSYSS SVFVRYDTS RLDVMKVLM TGPADTPYA NGCFEFDVF FPPDYPLSP MMINLETTG HHTIRFNPN LYNDGKVCL SVLNTWHGR PEEKWNAQT SNFLQVLVS IQSLILVPE PYFNEPGYE RSRGTPAGT QSSREYNAN VSQATVRWA MLEQILNPC LCFKDVIYT HFYLKRHEI LAQVEGWIK DLENDITEK RISRTANKR SPTANIDSF KKIFQQLKE QLTKLKPPE GVVEEDPAP KMETTSASV NGLEGNEQE MDADIDMEK MVKEMCEFD AT
- >TC009848 RA
atggctgag gatccttgg ttattaaac gaagacggt tacttaaat gcggacaca gacatcaag gaaatcatt taccacccg acactgaac gttattttg atatgtacc aacagtgga atcgttcgg gtgttggat gtcaactcg ggtgttgtt ctccaatcc agctatctc tcagcaata aaccaaaac gaagtaact tgtaagtac attcctgga caagacagg gtgctgttt tgtgatgga caagctatt ggagtcaga tcagactac aacagtgtg cttttactc gacagtatt ctgcagaag ccagtatcg gatcccaag aaggaaatc aagctcgaa ttaccacta tctgaggca atcatcttg aaacaaagc ttgacttca gccaacgtc agcggtatt gaacatgtg ataaacgaa ttaaccgac gttattttg aacgctcaa aaacagtcc aagaagggt attaaagct caaaaatgg aacactgtc tgtctccaa ctccctttg gagatgctc caaatggct gccatgagc agcgtctca gaactactc ctaaaaaac caacacacc ccagaattg agtgttgca tcagccgtg caagagcgc ctctcggag ctcagtggg gaacaaggg agcgcttca gaccgcagg tctatggcc agcgaggcc aaacgacgc gaaacgttc acccattgg ccccacatg gactacaaa tgggccttg ccggaccaa atggcccag gcgggcttc taccaccag cccaacgcg tctggggac gaccgcgcc atgtgcttc acttgcacc gtgtgcctg gtctgttgg gagcgcacc gacgagccc tggagcgag cacgagcgg cattcgccc agttgtccg tttgtcatg ggcgaatac acccagaat gtcccatta tcggtcact tacgccacc aacccggct attgacgcg acctatcga ggggtcaac gtgaaaatc cttggtacg agcaccttg agcaacctt ctgccatcg gcgaacgcc gaaggcctg atctcggtg ttcgacatc tcggggaag atctcaagg acacactct tttttcgtc acacaattt gattcccac attctggag aagtacaca caggatttc ggggtccca ggcacctgg agcgacctc gacgaagaa aagcgaata acatcattg gcgattgtg agcactgcg gagacgacg tccacgcgc cctactatt atttgcggc ctccaaatc cgatgtcgg gcccagaaa taccagtca ccggtgatt ggaagttac gacgatcag aataaaaac ttgatggag gtggtggag tcggcaacg tcttcgacg agtttcagt tgtttgtat ttggtcgtt tacgatttt atgtacagt aaggagaag gaggataag gatcacgag agtaaaccg aacgatgtg aacgccatc tctgaagtg actgcgccc aagaatgaa tcaacgcaa aacgagaac cagaaaaac agtgattac gtgcatccc gacgccgtc ttcgtcgag catttatcg tcgttttac aaaactttg atacctggt gaaagtgac gaggtgttt ctcccgcct ccgatgctc gttaacaaa ccccaacat tcaggtcct agagtatta cccattgac atgcaaatt accgaaact gatgcttcg caaaccgag ccttttata atcaaccag gaatattcg tttatgaac ggccagttg agcagcatt ctttcggag acgttcgaa aagagcatt tccgaccac attaccgaa atgaaaaac aacaaaacg gccaaaaaa ttgaattat agtcgagcg gtgcagtgt gtgtcgata ccggcgcct tataaatcg cgacaggac ttggagatt tccgatatt atcgcgacg caagacagt cgacatgtt ctagtcgtg ttgaaaagc tcgttggac ggtaaaagc gttttgata ctctattct ttggacttc tcacagaag atggtgaag ttggttgaa gagccggtt ttggtgcgc gaattgaat aataatgaa aagccgaca gaagttagt atgttgccg atggtggat aagttgggg gagtttgcc gaacaaggg aagaatgga gtcgtaggg aacatcgtc atggtgtgc gtcgatggg gctgtgaga atcgtggag ttgtcgacg atgaagacc atatgcatg gcgcagttg gagagcgag aagtttgtt tcagcggca tattgcaac agtttggag cgcttgtgc gcctccact gcgaaaggt tccctgcac ttctatgcc cttaacgac accgacaac gaatcctct gaagaccac gaggaagac gacttgttc gggatgacc accgacggt acatcatca aaccccgcc caaaccagc gattcactc gacggaagt gaaatgacg cgttgtttc cacgaccct cccgaaata atttctttg atagacttg aaacgattg cacgctttg tgtcaattc gaaccgtta cgagcaggg tattgcgct gtagttcct ccctgttgg agcgaaatc cagcaggct caaagacag agaaggtac ccccaaagc ctcaaaacc gactgcgac cagtatacg aaaacgtgg cgactgcaa acggacaca accacctgg gatgagcac atcttcgag atcacattg cccagtccc atgtctctg ggccacgtg gacgtgcac ttcagtctg caatcgtcg acggcgttg ccgcatgtc gaagtgacg cttttgcgg caaaacacg agcgggatt gggcacaaa aaagacgtt aagtttgct gtggacgaa accataact atagatatg ttgcaacag tgggccgat aatcccgtt atcagtcag gagtatcta agggcacac aatgccgat attctggca ggtccggtg aacattgcc tcgtgtttg gacttgtcg gaacaatgc ggtgttgtt actttgacc agtccgaaa ttgttcaag tcgaggaat cggaccttg ctgttgcac atcagggct gtttattcc aaagaagag gctaataac aaaactgct tccaagagg aaacgctcc gagagcaag gacaagacg ggtcagtgt gccagccac aaaaatgag ttttacatg gggtgtgat tgcattcac gagttgagt attacggtg tatagttcc aaacatact gaaattccg catgagagg agtcagagg aatttcatg ttggagtct atttcctgc gtccagtcg ctgctgcta acagcaatg aataattcg tcaaatgag tcggtttct ttgtcgttg gatgtactc acgtggatt gcgtccatt cgtttgatt aggaatagg agtaacaac ggggaggtg cctaatcag cagatggaa tttttatac gtgattgag tacaacttg gcgaagctc ctccaacag tgtcttctc ctcggcgaa cgaagcatt gcacataag tgcatgaag ttaatcgaa gtttgttgc aatggcgct aagaacatg agcgagacg gggcgctcc cacttcgag tttagcatc ctgaaggcc cttctctcg cttttgaac acaatcacc gatgtccgg tcacctggc ggcctccag tggatcttc gcacttctc cttaaagtc atttcgaaa aacgctcaa tacgttgca accaaatgc gtaactttg ttgaataaa atgtccgaa gagttagaa aatcgaacg aatccgtac cacttactt ctcaggagt cgttacggc ctatacggc actcctcta gaactagaa ttatttgat atcgaaccg ccaccctac gctaaaagt accagtaca tacgtcact tacgctaca gcggtgtct ggggacagc gccaatcag aataacgac tttttcgag aactattcg ttcaataaa gagagtatt aaccctaaa gatgtgttg tcaggcact gagacgaag ctgaagtgg cgaaattta gcaccaccg aaaatatac agagggtta atagaaacc gttcctttg catttcact tgcgtttcg gcaagtgaa gggacgaga ctagaaagg gccgatgcc tcaaataac atcaataac atcgttatc aacgagata ccgttttca attacgcca ttccatggc cagggctct ttagacaaa atggaccac agcaagttt aaatcctac ttggttgat tttgtggaa gaaccgacc gtcaccgtt aaagaatac aaaaattcg aatccacaa gcaaccact tcgagtgtg ggcacgtgg gacgataac ggctgtgaa gtgttaatc ggagagtta ccagtggat gtcggcaac cacctctta aagaaatta gattccatg tatcaagat ttgaaatct tcaatggaa aataaaagt agcgcagta aagaatgtg tggcaacca gagagttta ctttcgccg ccgactaat agtagtttg ccgtggcaa cacttacta gtaactccg ccacaacag gttattgta gtggaacgg atgcattcg ggggctcgc agatacgtg gttttggat ttcgggcaa ccgattttg ctcactgat attcttcta ccgtcttgc aatgacctg gtctctgtt agtattgac atttggctg aagagtgaa gatgttgat gggactcga ttggtggtt gctgcagat attgggtcg aagaatttg gtgttgaat gatctgcag ccggcgccg ctgtgccga tacatgaag attaccaca gttggtcgt tacggaatg actacagcg agatgcaga atccctatt ggttacttt tacggccac gtggtagtt ctccctgaa gaagtacca ccggatttg tcccaaaac gtttcgaac ttggcttgc actgactta gataagcaa ctaacaatt ttatcgaaa cttttcgaa gatatcagc tgtcgatac agtttggct tgctccaaa ttaaaagag cttttacga ccgttcttg gtggccgac atgaaaaat gcatctcat ctggcgact tatatgaac atcatgaaa gacaaagcc cttaatacc agcaacccc gaacacagt aaaatcttt agcgcttat caggaatca atcacatac cagcgtcag ttgaacacg attcgaaat gtgatgaat cgacttgaa gggagtatt agtaaaatg tcggtctcg ccgagaaat cctaaagac attccgaac gcctcaacc gataaactt agagccatc gctgaaggt ttgcttgag gtgctacta acgctggat tcagcaatc gaaatcgat cccgtcacc tgtcgcaag ctcttccaa gggctatgc gtgtcgcaa gcttcacgt ctgcagtta ctaaccgcc attttcctg gacaagtcc tgcaggacg aagagttac tggggcaat ttcctcgcc gacactttg accgaaatg ttcgcgaca tccaacaca acaaaattc ccccaagac cgcgttttc atcctcttg gcttacttg agccggaaa agccccgaa cggagcgcc gtcattgac gccggtctt cgagtggtt tcgcaaact ttagctcct ttgactgga gatcgctcc aatttactt gcggtctcg gtggatctc cctctactc tcctggctg ttgatgtat ctctcgctc cagttggac ctaagcaag aacagtctc caatcggcc acgagatgg gactgggtg tttggggaa atcgtcggg aaggtcaat gccgaaagt gcgaaaatc ggaagccgg aagaaaaat tacaaacgc atcagcaag caacaaatc gacaatttg gatgcggca cagaagctg gtccagtct tcggctcag gctttgtcg acgttgtcc aaccatgcg gcgtgtctc acatcgaag ttggatgcg gctctgaag acgcaacag tttctgatg aagaaagtt aagtacaag ttcatggat atgaagaat tcgccggcg gagaatcca ctcgcgccg aagaagctg aaggagacg gcggcggaa ccagtaatc gagaaaatt accgagaag aagatgccg cagcatatt gacgttaat cattgtttg actgtggcc aaagggctg ctgactttg gtgctctcg atggaccat tcgagcagt gccgacatg ctcttgctt tcgtttaaa gtaatggcc cgcctggtc agcatggct aaaatcaaa ttgggccag ctgatgaac caggagcag ctcttgaac ttgttccgc ttttgcctc gcttcgaaa attccatgg gcccctcat gctctcgcc tgtctcttg caagacgtc gttgaaaac gaaaacgac accgactct ggggcttcc acaagctgg gccactaac gatttcctc tctgacgtg gcaaacctg tgcgaattc acggacgac gagacattc gttcacgaa cctgtaata acagcgcca cctaaaact ggaaataac aacaaccag ttgccttcg gtttacgaa tccgacgat tcggattta gaggaaatt gttgacggg tttttggac aaggaagcc atgtcgact ccgaagaag ataccgaaa ccaacgaat tactcacac accactaat tcagtagcg ctggattcg agacttgat ctcagtgtt gacgtatca gcggaaata tcgctacgg aagttgatt aataaaaat actcaaggg ttgttgcaa agtgtcaac gcgcctgtg gttggtgac gaaagtgaa gatgcgtta gcgccatgg cctactgtg gtattgccg ctgcaaccg gagtctctt ctgagtaac actgcgatg ctgactcag tgcttcgat tcgttgttt gagaacttg ccgatgcaa gactcggca aacattgag cacattttg cagttgtgg ctgactttg aataacccg tacaaggac gataagttt gatccttca gccgttgct caaatcgtt ctcaaagaa agctcagtg aaggctttg atttcagcg atagcctgg actccgggc ttgtctcta attacgtgg tgtgtcgcc ttacagact ttgactttg gtctgtaat agtaatata acgtcagag tcttccctc tattggtca gattcgtac ggaatggcc tcttgtata gtcaatcac ccggatttc gtccaggtc ttcatgagg ttgttatcg gggactggg cttaccttc attgataaa gttttggcg ggaccttcg ttatgtaaa gctttgaac gattttctc gaccggctt ttgatgagg tgttacgtg gaaagtccc cgaagcaaa gcaggaaaa tcgttaaag actctcttg ttgaaaatt gtgcaccag ttagtgcta cctacaggg ccaattgcc ctacgacaa gggcctcta gatgtgcag tgtaaattg ttgaaaatt atgttagat atagattat tttaacgcc gatatgact atcgcgatg agtattttc gagagtacg tgtgttctg gttcattcg tattttgtt aatgttgat aaaattaaa tgtattaat atcggggag aagcacaac gtggtcacg acgacgttt aatgagatt tttgcctgt gttatgggc tcggacccc atcaaaatg gatctacag gtctccact gaagtgctc tttttgcgg ctcttgaag tttttgggg aaattggtg caaacacag tttagacaa ggcatcgat caaccggaa gcaatggac gttgagccc ttggcgact tcgcaaacc gacgaaagc aaggccgag caaatccaa caagactcg atgcgcaat cgtggcgaa aatttcgcc gatcttgtc ctccaatac cacgctaat attatgcgt ctgtgccag tctttagca gcttgcaaa tcctcgtca ttgtgtatg ctagcaaat gtctcgcaa aaagcggcc ttttccaac ttgagcgaa ccgagtaac gttggagac gcagtgttc cacgtttta gccctgtta gctaagcga acctctgat aaaaagttg gtgttacca ccgttgctt caattctta gcacatgcg cctcaactg agcgagcct ttgttgtgg ttcctattg caaattttg aacaatgaa gaggctatt cggaatttc ctcaccgca ggtggtatt gaaattttg ggccgaagt ttcgtaacg agtagcaac actccgaat accatttcc aagtcaggg actatttcc atagtcatg caacacttc agtgggatt aatcccact tctgacgct agcacagcc atcgcgtca gcatcggct tcaaggaag ctgcaacaa gcaaccatc gaaaataaa ttgtctttg gttaatttc gccccttgc tgcactata agttgccca tcagggaca gcccaaccg gcggaagtc ctaattcaa ggaacggct gcgacccac cgccgggca cgaactccc caatggagt taccacttt taccccgac gaagcccac actgaactc actttgcaa ctcccgagt gcgatttta ctgcgtgag gtgcatttg cagccacac ttgagtgcc ctggcgact tgtccttca gctgtagcc ttggaagtc tcagccaat ggtccttcg cgtttagtc cctgtgtgt ccgccactt cctaccagt ggtatggtt ttcatcagg ctacacttg ccggttccg gaagtcgtc aattgtgtc ttgattcgt ttgtatcgt ccacgtgat gctaacaat atcgggctt tcgcaaatc cgtcttttg ggtaatttg gcttttggc ggaaatacc aatcagaac ttggccgat ccggtcgaa gatgagtca cactgtcgc cacagttta ggctggtta aggttacta caccactgt tttacgtta ccgacagag caggatttg gcaaaacaa gtgatagcg tgtgcgtct ggagtaccg agattgttg agtacttgt tgcgagttg cttctagta ccatcccac gtgcttccg atctattta ccgtgtttg gagaaggtt ttaagggag ttgagtttg cacaatttg gaaaacgga aacaatgtt atcaaaatt ctgctgaac agtcggtcg agtatcata gaaccgcta aatttcgcc gataatgcc tggcaagac aggttgttg ataaatgca acggggtac caatcagct tgtgaattg ttgtaccag atttgcgaa catcaagat tctgatact ttctaccgc gttaagatg atgctggag tggttgcaa agtgtcgca gtcgaagcg atgcaaacc ggaaatacg caagggtgc aaccctgcg tacatatct agcatagct tcgattctg tggtcggcc aatcaggct cgagtcagc tacgactta ccgtcaatg atcacttta gatttcttc gacacgatc tacaacctt aaaacctgc gtggaaaac aacatcgcg cttaaatac tccctcgac tccctgttg tgcagtttg tgttacata aggcctgaa cttttcccg gtgctttta caacgaatc ggagtgtta gttccgaat ttgtccacc gatcatgac gcttcgatc tccgacgac cgcaaagac tccgaaagt atgaccgac gataacaaa caaaccttc gagctagat tcggaatgg tacggccac ctggtaatg ggcgaactc tcgaaactt agtctttcg aacgaacaa ctggaaacc gtggccttg gtgagtcgc tcaccctcg gccatccaa cagttgcta gactccggt ctcccgaag atgttgaac agtgccata tacgagttt tgtacttca aacgaggat aattcagtc ccaatggct aaactggag aacgtgact gcgatttta aagttcttc actgatgtt agcgacgag aaaacgatg cgtgattgg ctggggtcg tccgatggc tcgtcgttt tggctcgag ttgctcaac tggttgtgc aaaaaaccg ttcctgaaa aagtcgaat ctccagtca gaagcgcac gcccaattg gaggaagtg tgtgtcaag tttttgtcg aagtgttgc ctttgtcac cctagtaat caagcgcga ctggcgaat gttttatgc aaagttata gatttgcaa agtaatgga atctccggt ttcatgcgt cggctgact cttcagcta ctgctggaa aacgagaag atccccgtc agtattaaa gcggacgag actttgtat aaaagcgtc aagttgact caggcgtac ttgccggtg catccggcc ttcaaacag acctacaac cgggctttg ctctacttg agcactaat acgactcta gccgacatc ctagagcag cacatttat tttaacacg acctacaag atggagacg aataactcg agtaagcga ccttttgtc actaagaaa gatctattc aaaggttgg ttccccgaa gattcagac ttgagcatg gctgcgggt gttactgcc aaagataaa cgtgccaaa gacgccaaa aatcaagcc gcctcgacg ccccagtcg aagaagaag aggtacacg gcgacggaa agtctgact agtggggac gttattggg ggacgcctg atcaagtgc gaggcgtac tcggaccag cccttgccc ctcaacttg aatctgggg caactgctg cggttgatt gagtcgaag gggacgact gccgattgg ccgtgtgtc catttagtc gtttgtcaa aacaaaaat ggtgataaa aaaagtgaa agtagtgaa gtagattct agtttgtac cagcaacaa ccattcagt agtgcctta caagtgttc agttcgatg ggtgggttg gcgctactg gctcggcat ttgcctacg gtgtaccca gaggcagtg cggccacca cttaaggag aaaaccacg tccgaacaa tcggactcc gagtggatt aaagttgaa gactgcgac gatatctac gaagatgtc gaagaaaca gtcgggacc agttcgcct tcaaagtca tcagggctt atttcaaac gttccagct cactctctc actgccttt ggtctcttt ctccgactg ccgggctac gctgaagtc cttctcaaa gacatgaag aaggctcta tgtctgttg cgcttagta ttgggtgtg accgacgac ggcgaaggc ggtgatata ttccagtcc ccgattgcc gactcgctc cccacgttg ccttttgaa gtgctccga aagctctac gatgccaca cctttgagc accgacgat ggccggctt ctccgacgc atcagcatt aatacgggc gtggtccac ttgctctta gcttgtttg gggattttc acccatcag actcaaaac aacgagaaa gagggccag aaagacgca aaagggaag gacgaacgc tcccagttg tactgggcc aaaggtaca ggctacggt acgggttcg actcaacaa atctggaac gttgaacaa gccctactt aaacaaaag agcgaagag gagcacgtg acagtcctg ttacaagtt ttggcgagc tatatcagt cccaacggg gaaactgcg gatgagtta atggaagat gttttgccg tcgtcgttt catgacctt ttggccaat agctcgctt ttgccggcg cttagctcg tatctgagg aacgattca gttttagat atggcccgg catataccg ctctacaga gcggccttg caactatta agagccttg gctactagt tcgcaactg gtggatttg ctcttgcct caaaaaacc cgcatcaac gaaccatct attagttca ttattgaaa agcatgaag acttgcgtt gatacttat gcttctaaa ttaagactc aacactaaa acaaataca aaattcaaa accaaactt cgggaacaa ctcgaagaa cttgaacag ggtgaaggt ctggcgact ttaatgccc gacatccag gacaccgcc aatttggtg tctaaggta acaagcggc ctcgtagac tcggaccac gatagcgaa ggggaagtt gccgtggat cgggtcata tttgcgtct ttggaagag aaatatctc aagataatg aagagtctg cagtttggt tcgtacgaa atgatcacg gaattgcct gaaggtggt gtaaagttt gcggtgtcg caccatttt gaaaacaac gcgaaatcg acgggggag cagtcgcac ccggcgcgt gtcaagcgg atagcgcag gaggccgta accctatcc acgagtctg cctttgagt tacagcagt tctgttttc gttcgttac gacacctcc cggttggac gtcatgaag gtgctgatg accggccct gcggacact ccctacgcc aacggctgc ttcgaattc gacgttttt ttcccgccg gattacccc ttgtccccc atgatgatc aacctggaa acgacggga caccacact attaggttt aatccgaat ttatataac gacggcaag gtttgtctg tccgtgctg aacacctgg cacggacgg cctgaggag aagtggaac gcccaaacc tccaacttc ctgcaggtc ctggtgtct atccagagc ctgatcctt gtgccagag ccctacttc aacgagccg ggctatgaa aggtcccgc gggacgccg gccggcacg cagagttct agagaatac aacgcgaac gtgtcccaa gccacggtc aggtgggcc atgttagaa caaattctg aacccttgt ttgtgtttt aaagatgtt atttacacg catttctat ttgaaacgt cacgagatc ttggcgcag gtcgagggt tggatcaaa gacctagag aacgatatc acggagaag aggatatcg cggactgcg aataagagg tcgccgacg gcgaatatc gacagtttc aaaaagata ttccagcag ttgaaggag cagttgacc aagttgaag ccgccggaa ggcgtggtg gaggaagac ccggccccg aagatggag accacgagt gcgtcggtt aacgggctg gagggcaac gagcaggag atggacgcg gatatcgac atggagaag atggttaag gaaatgtgc gagtttgac gcaacatag
- >TC009848 RA
taacttcca caaatggtg gttttcaat ggcgcaaga tgtgtttag caaaatggc tgaggatcc ttggttatt aaacgaaga cggttactt aaatgcgga cacagacat caaggaaat catttacca cccgacact gaacgttat tttgatatg taccaacag tggaatcgt tcgggtgtt ggatgtcaa ctcgggtgt tgttctcca atccagcta tctctcagc aataaacca aaacgaagt aacttgtaa gtacattcc tggacaaga cagggtgct gttttgtga tggacaagc tattggagt cagatcaga ctacaacag tgtgctttt actcgacag tattctgca gaagccagt atcggatcc caagaagga aatcaagct cgaattacc actatctga ggcaatcat cttgaaaca aagcttgac ttcagccaa cgtcagcgg tattgaaca tgtgataaa cgaattaac cgacgttat tttgaacgc tcaaaaaca gtccaagaa gggtattaa agctcaaaa atggaacac tgtctgtct ccaactccc tttggagat gctccaaat ggctgccat gagcagcgt ctcagaact actcctaaa aaaccaaca caccccaga attgagtgt tgcatcagc cgtgcaaga gcgcctctc ggagctcag tggggaaca agggagcgc ttcagaccg caggtctat ggccagcga ggccaaacg acgcgaaac gttcaccca ttggcccca catggacta caaatgggc cttgccgga ccaaatggc ccaggcggg cttctacca ccagcccaa cgcgtctgg ggacgaccg cgccatgtg cttcacttg caccgtgtg cctggtctg ttgggagcg caccgacga gccctggag cgagcacga gcggcattc gcccagttg tccgtttgt catgggcga atacaccca gaatgtccc attatcggt cacttacgc caccaaccc ggctattga cgcgaccta tcgaggggt caacgtgaa aatccttgg tacgagcac cttgagcaa ccttctgcc atcggcgaa cgccgaagg cctgatctc ggtgttcga catctcggg gaagatctc aaggacaca ctctttttt cgtcacaca atttgattc ccacattct ggagaagta cacacagga tttcggggt cccaggcac ctggagcga cctcgacga agaaaagcg aataacatc attggcgat tgtgagcac tgcggagac gacgtccac gcgccctac tattatttg cggcctcca aatccgatg tcgggccca gaaatacca gtcaccggt gattggaag ttacgacga tcagaataa aaacttgat ggaggtggt ggagtcggc aacgtcttc gacgagttt cagttgttt gtatttggt cgtttacga ttttatgta cagtaagga gaaggagga taaggatca cgagagtaa accgaacga tgtgaacgc catctctga agtgactgc gcccaagaa tgaatcaac gcaaaacga gaaccagaa aaacagtga ttacgtgca tcccgacgc cgtcttcgt cgagcattt atcgtcgtt ttacaaaac tttgatacc tggtgaaag tgacgaggt gtttctccc gcctccgat gctcgttaa caaacccca acattcagg tcctagagt attacccat tgacatgca aattaccga aactgatgc ttcgcaaac cgagccttt tataatcaa ccaggaata ttcgtttat gaacggcca gttgagcag cattctttc ggagacgtt cgaaaagag catttccga ccacattac cgaaatgaa aaacaacaa aacggccaa aaaattgaa ttatagtcg agcggtgca gtgtgtgtc gataccggc gccttataa atcgcgaca ggacttgga gatttccga tattatcgc gacgcaaga cagtcgaca tgttctagt cgtgttgaa aagctcgtt ggacggtaa aagcgtttt gatactcta ttctttgga cttctcaca gaagatggt gaagttggt tgaagagcc ggttttggt gcgcgaatt gaataataa tgaaaagcc gacagaagt tagtatgtt gccgatggt ggataagtt gggggagtt tgccgaaca agggaagaa tggagtcgt agggaacat cgtcatggt gtgcgtcga tggggctgt gagaatcgt ggagttgtc gacgatgaa gaccatatg catggcgca gttggagag cgagaagtt tgtttcagc ggcatattg caacagttt ggagcgctt gtgcgcctc cactgcgaa aggttccct gcacttcta tgcccttaa cgacaccga caacgaatc ctctgaaga ccacgagga agacgactt gttcgggat gaccaccga cggtacatc atcaaaccc cgcccaaac cagcgattc actcgacgg aagtgaaat gacgcgttg tttccacga ccctcccga aataatttc tttgataga cttgaaacg attgcacgc tttgtgtca attcgaacc gttacgagc agggtattg cgctgtagt tcctccctg ttggagcga aatccagca ggctcaaag acagagaag gtaccccca aagcctcaa aaccgactg cgaccagta tacgaaaac gtggcgact gcaaacgga cacaaccac ctgggatga gcacatctt cgagatcac attgcccag tcccatgtc tctgggcca cgtggacgt gcacttcag tctgcaatc gtcgacggc gttgccgca tgtcgaagt gacgctttt gcggcaaaa cacgagcgg gattgggca caaaaaaga cgttaagtt tgctgtgga cgaaaccat aactataga tatgttgca acagtgggc cgataatcc cgttatcag tcaggagta tctaagggc acacaatgc cgatattct ggcaggtcc ggtgaacat tgcctcgtg tttggactt gtcggaaca atgcggtgt tgttacttt gaccagtcc gaaattgtt caagtcgag gaatcggac cttgctgtt gcacatcag ggctgttta ttccaaaga agaggctaa taacaaaac tgcttccaa gaggaaacg ctccgagag caaggacaa gacgggtca gtgtgccag ccacaaaaa tgagtttta catggggtg tgattgcat tcacgagtt gagtattac ggtgtatag ttccaaaca tactgaaat tccgcatga gaggagtca gaggaattt catgttgga gtctatttc ctgcgtcca gtcgctgct gctaacagc aatgaataa ttcgtcaaa tgagtcggt ttctttgtc gttggatgt actcacgtg gattgcgtc cattcgttt gattaggaa taggagtaa caacgggga ggtgcctaa tcagcagat ggaattttt atacgtgat tgagtacaa cttggcgaa gctcctcca acagtgtct tctcctcgg cgaacgaag cattgcaca taagtgcat gaagttaat cgaagtttg ttgcaatgg cgctaagaa catgagcga gacggggcg ctcccactt cgagtttag catcctgaa ggcccttct ctcgctttt gaacacaat caccgatgt ccggtcacc tggcggcct ccagtggat cttcgcact tctccttaa agtcatttc gaaaaacgc tcaatacgt tgcaaccaa atgcgtaac tttgttgaa taaaatgtc cgaagagtt agaaaatcg aacgaatcc gtaccactt acttctcag gagtcgtta cggcctata cggcactcc tctagaact agaattatt tgatatcga accgccacc ctacgctaa aagtaccag tacatacgt cacttacgc tacagcggt gtctgggga cagcgccaa tcagaataa cgacttttt cgagaacta ttcgttcaa taaagagag tattaaccc taaagatgt gttgtcagg cactgagac gaagctgaa gtggcgaaa tttagcacc accgaaaat atacagagg gttaataga aaccgttcc tttgcattt cacttgcgt ttcggcaag tgaagggac gagactaga aagggccga tgcctcaaa taacatcaa taacatcgt tatcaacga gataccgtt ttcaattac gccattcca tggccaggg ctctttaga caaaatgga ccacagcaa gtttaaatc ctacttggt tgattttgt ggaagaacc gaccgtcac cgttaaaga atacaaaaa ttcgaatcc acaagcaac cacttcgag tgtgggcac gtgggacga taacggctg tgaagtgtt aatcggaga gttaccagt ggatgtcgg caaccacct cttaaagaa attagattc catgtatca agatttgaa atcttcaat ggaaaataa aagtagcgc agtaaagaa tgtgtggca accagagag tttactttc gccgccgac taatagtag tttgccgtg gcaacactt actagtaac tccgccaca acaggttat tgtagtgga acggatgca ttcgggggc tcgcagata cgtggtttt ggatttcgg gcaaccgat tttgctcac tgatattct tctaccgtc ttgcaatga cctggtctc tgttagtat tgacatttg gctgaagag tgaagatgt tgatgggac tcgattggt ggttgctgc agatattgg gtcgaagaa tttggtgtt gaatgatct gcagccggc gccgctgtg ccgatacat gaagattac cacagttgg tcgttacgg aatgactac agcgagatg cagaatccc tattggtta cttttacgg ccacgtggt agttctccc tgaagaagt accaccgga tttgtccca aaacgtttc gaacttggc ttgcactga cttagataa gcaactaac aattttatc gaaactttt cgaagatat cagctgtcg atacagttt ggcttgctc caaattaaa agagctttt acgaccgtt cttggtggc cgacatgaa aaatgcatc tcatctggc gacttatat gaacatcat gaaagacaa agcccttaa taccagcaa ccccgaaca cagtaaaat ctttagcgc ttatcagga atcaatcac ataccagcg tcagttgaa cacgattcg aaatgtgat gaatcgact tgaagggag tattagtaa aatgtcggt ctcgccgag aaatcctaa agacattcc gaacgcctc aaccgataa acttagagc catcgctga aggtttgct tgaggtgct actaacgct ggattcagc aatcgaaat cgatcccgt cacctgtcg caagctctt ccaagggct atgcgtgtc gcaagcttc acgtctgca gttactaac cgccatttt cctggacaa gtcctgcag gacgaagag ttactgggg caatttcct cgccgacac tttgaccga aatgttcgc gacatccaa cacaacaaa attccccca agaccgcgt tttcatcct cttggctta cttgagccg gaaaagccc cgaacggag cgccgtcat tgacgccgg tcttcgagt ggtttcgca aactttagc tcctttgac tggagatcg ctccaattt acttgcggt ctcggtgga tctccctct actctcctg gctgttgat gtatctctc gctccagtt ggacctaag caagaacag tctccaatc ggccacgag atgggactg ggtgtttgg ggaaatcgt cgggaaggt caatgccga aagtgcgaa aatcggaag ccggaagaa aaattacaa acgcatcag caagcaaca aatcgacaa tttggatgc ggcacagaa gctggtcca gtcttcggc tcaggcttt gtcgacgtt gtccaacca tgcggcgtg tctcacatc gaagttgga tgcggctct gaagacgca acagtttct gatgaagaa agttaagta caagttcat ggatatgaa gaattcgcc ggcggagaa tccactcgc gccgaagaa gctgaagga gacggcggc ggaaccagt aatcgagaa aattaccga gaagaagat gccgcagca tattgacgt taatcattg tttgactgt ggccaaagg gctgctgac tttggtgct ctcgatgga ccattcgag cagtgccga catgctctt gctttcgtt taaagtaat ggcccgcct ggtcagcat ggctaaaat caaattggg ccagctgat gaaccagga gcagctctt gaacttgtt ccgcttttg cctcgcttc gaaaattcc atgggcccc tcatgctct cgcctgtct cttgcaaga cgtcgttga aaacgaaaa cgacaccga ctctggggc ttccacaag ctgggccac taacgattt cctctctga cgtggcaaa cctgtgcga attcacgga cgacgagac attcgttca cgaacctgt aataacagc gccacctaa aactggaaa taacaacaa ccagttgcc ttcggttta cgaatccga cgattcgga tttagagga aattgttga cgggttttt ggacaagga agccatgtc gactccgaa gaagatacc gaaaccaac gaattactc acacaccac taattcagt agcgctgga ttcgagact tgatctcag tgttgacgt atcagcgga aatatcgct acggaagtt gattaataa aaatactca agggttgtt gcaaagtgt caacgcgcc tgtggttgg tgacgaaag tgaagatgc gttagcgcc atggcctac tgtggtatt gccgctgca accggagtc tcttctgag taacactgc gatgctgac tcagtgctt cgattcgtt gtttgagaa cttgccgat gcaagactc ggcaaacat tgagcacat tttgcagtt gtggctgac tttgaataa cccgtacaa ggacgataa gtttgatcc ttcagccgt tgctcaaat cgttctcaa agaaagctc agtgaaggc tttgatttc agcgatagc ctggactcc gggcttgtc tctaattac gtggtgtgt cgccttaca gactttgac tttggtctg taatagtaa tataacgtc agagtcttc cctctattg gtcagattc gtacggaat ggcctcttg tatagtcaa tcacccgga tttcgtcca ggtcttcat gaggttgtt atcggggac tgggcttac cttcattga taaagtttt ggcgggacc ttcgttatg taaagcttt gaacgattt tctcgaccg gcttttgat gaggtgtta cgtggaaag tccccgaag caaagcagg aaaatcgtt aaagactct cttgttgaa aattgtgca ccagttagt gctacctac agggccaat tgccctacg acaagggcc tctagatgt gcagtgtaa attgttgaa aattatgtt agatataga ttattttaa cgccgatat gactatcgc gatgagtat tttcgagag tacgtgtgt tctggttca ttcgtattt tgttaatgt tgataaaat taaatgtat taatatcgg ggagaagca caacgtggt cacgacgac gtttaatga gatttttgc ctgtgttat gggctcgga ccccatcaa aatggatct acaggtctc cactgaagt gctcttttt gcggctctt gaagttttt ggggaaatt ggtgcaaac acagtttag acaaggcat cgatcaacc ggaagcaat ggacgttga gcccttggc gacttcgca aaccgacga aagcaaggc cgagcaaat ccaacaaga ctcgatgcg caatcgtgg cgaaaattt cgccgatct tgtcctcca ataccacgc taatattat gcgtctgtg ccagtcttt agcagcttg caaatcctc gtcattgtg tatgctagc aaatgtctc gcaaaaagc ggccttttc caacttgag cgaaccgag taacgttgg agacgcagt gttccacgt tttagccct gttagctaa gcgaacctc tgataaaaa gttggtgtt accaccgtt gcttcaatt cttagcaca tgcgcctca actgagcga gcctttgtt gtggttcct attgcaaat tttgaacaa tgaagaggc tattcggaa tttcctcac cgcaggtgg tattgaaat tttgggccg aagtttcgt aacgagtag caacactcc gaataccat ttccaagtc agggactat ttccatagt catgcaaca cttcagtgg gattaatcc cacttctga cgctagcac agccatcgc gtcagcatc ggcttcaag gaagctgca acaagcaac catcgaaaa taaattgtc tttggttaa tttcgcccc ttgctgcac tataagttg cccatcagg gacagccca accggcgga agtcctaat tcaaggaac ggctgcgac ccaccgccg ggcacgaac tccccaatg gagttacca cttttaccc cgacgaagc ccacactga actcacttt gcaactccc gagtgcgat tttactgcg tgaggtgca tttgcagcc acacttgag tgccctggc gacttgtcc ttcagctgt agccttgga agtctcagc caatggtcc ttcgcgttt agtccctgt gtgtccgcc acttcctac cagtggtat ggttttcat caggctaca cttgccggt tccggaagt cgtcaattg tgtcttgat tcgtttgta tcgtccacg tgatgctaa caatatcgg gctttcgca aatccgtct tttgggtaa tttggcttt tggcggaaa taccaatca gaacttggc cgatccggt cgaagatga gtcacactg tcgccacag tttaggctg gttaaggtt actacacca ctgttttac gttaccgac agagcagga tttggcaaa acaagtgat agcgtgtgc gtctggagt accgagatt gttgagtac ttgttgcga gttgcttct agtaccatc ccacgtgct tccgatcta tttaccgtg tttggagaa ggttttaag ggagttgag tttgcacaa tttggaaaa cggaaacaa tgttatcaa aattctgct gaacagtcg gtcgagtat catagaacc gctaaattt cgccgataa tgcctggca agacaggtt gttgataaa tgcaacggg gtaccaatc agcttgtga attgttgta ccagatttg cgaacatca agattctga tactttcta ccgcgttaa gatgatgct ggagtggtt gcaaagtgt cgcagtcga agcgatgca aaccggaaa tacgcaagg gtgcaaccc tgcgtacat atctagcat agcttcgat tctgtggtc ggccaatca ggctcgagt cagctacga cttaccgtc aatgatcac tttagattt cttcgacac gatctacaa ccttaaaac ctgcgtgga aaacaacat cgcgcttaa atactccct cgactccct gttgtgcag tttgtgtta cataaggcc tgaactttt cccggtgct tttacaacg aatcggagt gttagttcc gaatttgtc caccgatca tgacgcttc gatctccga cgaccgcaa agactccga aagtatgac cgacgataa caaacaaac cttcgagct agattcgga atggtacgg ccacctggt aatgggcga actctcgaa acttagtct ttcgaacga acaactgga aaccgtggc cttggtgag tcgctcacc ctcggccat ccaacagtt gctagactc cggtctccc gaagatgtt gaacagtgc catatacga gttttgtac ttcaaacga ggataattc agtcccaat ggctaaact ggagaacgt gactgcgat tttaaagtt cttcactga tgttagcga cgagaaaac gatgcgtga ttggctggg gtcgtccga tggctcgtc gttttggct cgagttgct caactggtt gtgcaaaaa accgttcct gaaaaagtc gaatctcca gtcagaagc gcacgccca attggagga agtgtgtgt caagttttt gtcgaagtg ttgcctttg tcaccctag taatcaagc gcgactggc gaatgtttt atgcaaagt tatagattt gcaaagtaa tggaatctc cggtttcat gcgtcggct gactcttca gctactgct ggaaaacga gaagatccc cgtcagtat taaagcgga cgagacttt gtataaaag cgtcaagtt gactcaggc gtacttgcc ggtgcatcc ggccttcaa acagaccta caaccgggc tttgctcta cttgagcac taatacgac tctagccga catcctaga gcagcacat ttattttaa cacgaccta caagatgga gacgaataa ctcgagtaa gcgaccttt tgtcactaa gaaagatct attcaaagg ttggttccc cgaagattc agacttgag catggctgc gggtgttac tgccaaaga taaacgtgc caaagacgc caaaaatca agccgcctc gacgcccca gtcgaagaa gaagaggta cacggcgac ggaaagtct gactagtgg ggacgttat tgggggacg cctgatcaa gtgcgaggc gtactcgga ccagccctt gcccctcaa cttgaatct ggggcaact gctgcggtt gattgagtc gaaggggac gactgccga ttggccgtg tgtccattt agtcgtttg tcaaaacaa aaatggtga taaaaaaag tgaaagtag tgaagtaga ttctagttt gtaccagca acaaccatt cagtagtgc cttacaagt gttcagttc gatgggtgg gttggcgct actggctcg gcatttgcc tacggtgta cccagaggc agtgcggcc accacttaa ggagaaaac cacgtccga acaatcgga ctccgagtg gattaaagt tgaagactg cgacgatat ctacgaaga tgtcgaaga aacagtcgg gaccagttc gccttcaaa gtcatcagg gcttatttc aaacgttcc agctcactc tctcactgc ctttggtct ctttctccg actgccggg ctacgctga agtccttct caaagacat gaagaaggc tctatgtct gttgcgctt agtattggg tgtgaccga cgacggcga aggcggtga tatattcca gtccccgat tgccgactc gctccccac gttgccttt tgaagtgct ccgaaagct ctacgatgc cacaccttt gagcaccga cgatggccg gcttctccg acgcatcag cattaatac gggcgtggt ccacttgct cttagcttg tttggggat tttcaccca tcagactca aaacaacga gaaagaggg ccagaaaga cgcaaaagg gaaggacga acgctccca gttgtactg ggccaaagg tacaggcta cggtacggg ttcgactca acaaatctg gaacgttga acaagccct acttaaaca aaagagcga agaggagca cgtgacagt cctgttaca agttttggc gagctatat cagtcccaa cggggaaac tgcggatga gttaatgga agatgtttt gccgtcgtc gtttcatga ccttttggc caatagctc gcttttgcc ggcgcttag ctcgtatct gaggaacga ttcagtttt agatatggc ccggcatat accgctcta cagagcggc cttgcaact attaagagc cttggctac tagttcgca actggtgga tttgctctt gcctcaaaa aacccgcat caacgaacc atctattag ttcattatt gaaaagcat gaagacttg cgttgatac ttatgcttc taaattaag actcaacac taaaacaaa tacaaaatt caaaaccaa acttcggga acaactcga agaacttga acagggtga aggtctggc gactttaat gcccgacat ccaggacac cgccaattt ggtgtctaa ggtaacaag cggcctcgt agactcgga ccacgatag cgaagggga agttgccgt ggatcgggt catatttgc gtctttgga agagaaata tctcaagat aatgaagag tctgcagtt tggttcgta cgaaatgat cacggaatt gcctgaagg tggtgtaaa gtttgcggt gtcgcacca ttttgaaaa caacgcgaa atcgacggg ggagcagtc gcacccggc gcgtgtcaa gcggatagc gcaggaggc cgtaaccct atccacgag tctgccttt gagttacag cagttctgt tttcgttcg ttacgacac ctcccggtt ggacgtcat gaaggtgct gatgaccgg ccctgcgga cactcccta cgccaacgg ctgcttcga attcgacgt ttttttccc gccggatta ccccttgtc ccccatgat gatcaacct ggaaacgac gggacacca cactattag gtttaatcc gaatttata taacgacgg caaggtttg tctgtccgt gctgaacac ctggcacgg acggcctga ggagaagtg gaacgccca aacctccaa cttcctgca ggtcctggt gtctatcca gagcctgat ccttgtgcc agagcccta cttcaacga gccgggcta tgaaaggtc ccgcgggac gccggccgg cacgcagag ttctagaga atacaacgc gaacgtgtc ccaagccac ggtcaggtg ggccatgtt agaacaaat tctgaaccc ttgtttgtg ttttaaaga tgttattta cacgcattt ctatttgaa acgtcacga gatcttggc gcaggtcga gggttggat caaagacct agagaacga tatcacgga gaagaggat atcgcggac tgcgaataa gaggtcgcc gacggcgaa tatcgacag tttcaaaaa gatattcca gcagttgaa ggagcagtt gaccaagtt gaagccgcc ggaaggcgt ggtggagga agacccggc cccgaagat ggagaccac gagtgcgtc ggttaacgg gctggaggg caacgagca ggagatgga cgcggatat cgacatgga gaagatggt taaggaaat gtgcgagtt tgacgcaac atagatcaa ctgtttgca tttattttg tttttacca aaatttaga caatacatt tttacaaat tcccatagc ttttcggta tttctcatc gttgattca tcaatacgt cttggtaat cgtattatg atttttaca ttattatta aaactgcat ggctgatca gagaagtgg ttaccagtt ttacttgtg tcataagtg attttttta cagtgattt gtaattaaa ttttttgta ttatgactg taaagttcg ttcatttga aattactgt taagacaca aatgtgatt taaggaaaa taaaataat tttgtttta
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