| General information | |||
| TC009013 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 8768501..8783828 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC009013 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02857 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
Fertility
egg production: number <50% of expected -
mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 2
ovary without detectable defects - Number of animals showing the phenotype: 2
mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 2
ovary without detectable defects - Number of animals showing the phenotype: 2
- >TC009013 RA
MVTVCFKQK STLIKCLIF LVSVLIMIL VGLAIVLVK RNEDHHVCK SDHCIKLSA ELLRSLDQS RHPCDDFYA FTCGGLSRN RKEIPPAST KEVLNFMVG EMVQSTDSK PFIMEKSFY LACSNLAGI ELDDDEWLL DQFFSLGDW PVLRGSNWD GSYFEWTEI VKKSRRLGM KIDWFVDFS IYEDVNDTR VNVLKIDIP ALTQLKESE VFPRQKLLT KIAVTLGAP EKLAHYETG RTIEFEKEL RMLAHVYRS RKTHMNANT LPKIPISEV QKLCPHISW LDLINAVTG VNMQETDVV IFDEMVDYI KDLNKLIYG TPKRIVADY MMWKVVEEN WQFLTQPVR QKFEEYYEE QHQIQPELD RQKFCINAS RKQFPLIVE SEYIRRHVT LQKRQLVKE MLINIQRNY RRLLHSATW MNRKRRELA LGKLDELKF LIGGPREVF DEFGFAEDL GLYEFVFKS TNIIDMLNQ RKLRFYDHY FSSVNKAVT NRTQKFYEK VININRFTV INDDTLVIP AALLKDNFY DPNLPNNFN YATLGKSLA HKIFEHIFE RNNVTIIDE ETQNYNTWW ISDGFDQAV KCLIHEASD FHNPELKRQ KKMRELLAH FVSTEVAYE SFKELLTYR QDSPQSKLQ SLQYTPEQL FWIHTNMNP TNYEDERRL HGCKKNKLA TCLAITITT LIFALFFGF FGYFGFPEI STYCNTPEC LNASAELSE IIDDSIDPC TDFYSFVCG KLIKNQKTQ QNSLKQEYE KIEQTLDAL IVGPETPDD SQEIRMQKQ YFQACLNTT NIDSNKEIT LIRELNSVG GWPFLQNSR WIEKEFDWI KFMIRARKL RLPFEWFLN VGLFFDRGQ YQLKLSHPD DCGLNINED YFTMQRKIL EQLRKPGID AIVEFTQLE NFERKLNSI CANMTDNDI SVRRASNLW LDGIGPISN FLTSVTGLR GWKSNSWVA YNHNYLFEL NHLLKLFSK KTQALFILW KIIHFYAPY LSKPIREKY FNYLEIMMQ RNISREEFC LEDTKEKFK YVSEIKYLT ETVDPETRE EGKGFVASL VNEITDRIE NDTIRRLHS ETYSDLEEV IVGPEEIFD DTFIEEYLQ IGQLHFFTD NIILMLNTW SQRIFEKKM SMIGKPFLS LDSIFANDG EYSMTLDEL LSVPSVTFY TFFNTDVQK YLEYGSLGT IVGTEIVRL EHYLNGGNQ KLETNKNCM IENIKSFNR RFNASIVYE KNFLAWLGQ ELSYQAYQK YTDEEGAEA PLPDLPYTP NQLFWIKSS LTWCQAALL SPENSFRTF QMLRIKVTE SVKHSEFFG KDFNCPTDS PMNPENKCQ LFVYHVSEK PKSGKKWTR LELFLALVV IGLGIYVFY LRSYGQNDE ELTNKCGDI SCYKTVTEL QKFIEPSVD PCEDFHQFS CGKFLETVH TQEITSRLD QEQTKLENE LEAMIVGPI LNKNSRPVQ IQKMFFQSC LNLEDIEAD KNGKFLSLV KEIGGWPLV EGKKWNESN FDWKEVAVK CRKLGLPFG WFLEFGVYE EDRNYLKIA CPTHVNRTN SVDKIIVNL MGKKANIKD VIDFERRLL HISRPETPE INLRTISEL QKIHPPVDW LDFLNNITE ESYEFTEET EIASNIDDY ILRLHMLLT STPKSTQAN YIVWKLLEH FAPFIQSGV EKKLDRFEF CKQTTKEMF PDFSPIEYI RKHPTSKAR KSVKEIADL VKKEFVVRI QDATWWNET EKQVYLELF QNSGFLIGN RIENFEDDF LDDQYLHVK TMILEENSD VIEILTAKS RSYFNSIFG KINESSFIR EESFSEFMK VDIFFDKNL NKMVLPPPA LHGLLFDHS RPQFINFAS LGDSLAKEY VNTLRILTH NSSDVVFQT SDKCLEADY LSLGGKNEF PSELISDIL GRDISYKAY QKWIATHVD ESQLPLLPY TPNQLFWIK SSLINCQLI DSTLNHLSI RHSRHFGKD FSCDSSPMN PREKLTEPQ FCVTLSCVE SSKIMNKMV DPRKNPCED FHAYVCGKS QSPRDDSQR VLKEVLSEG DKVNDTASL KLQKQFYHS CNDLKAIDM DNDTTFAHL IAKLGGWPV VSGNNWNES AFDFAEFMV NLRELGFQY DWFLDVSVY SNLGENIIL EINVPDNVN KIEEKDEQN YADLMVDVA MAFGAHKSV AKSQIPEVV YLERKLANL VDEIQTENE ELAKQKSFH LLTVAEVQQ KWQKLNWLE FLNHITQKK LSDTQLVAF YVESYMPKL DKLLKQTTK RTQVNYVIW KLIETFSPY LSKPIRDLM QNYSIKANE TETWYQDRS TFCFEESKS FFQYSLEAE FVRRYVTAT KRRNVEELI ERILTHLEE HFRDSKWLD FETRKAAIY KIQNISYII GGPEEMYYD KQFDKDFGI GELEFNSSN IIHINMKLF EKGLDLLFN KKYKETNYL KYTFYELVP SREIRFMQR FNLIWVPAG ALHGYLYED TRPNYLNYG TLGTTIAQQ LIEAMYFSI LRNVSEVTF SNFKNMTHC LPRCGSDSE LIDCNESLI TIMSNYLAV DIAYLAYKR WEKQNFVEK NLHGLHFSG DQLFWLAYV CILIALFCV FTLHVKNGN RHFTRPVVQ NVSCPTPSP LRVKKSTPC MTQSCIKAA SQIIDFIDP SVNPCDNFY DFVCGNFLK KPKVSNEIT PLSALEAVT LQQFNSLIT EPINETLPK SWALQRKYY NTCMNTSAI EENNSESFL EMLKFLGDW PLLHGRFWD ERKFDWLNS MVLCRKVGF TFEYFLKIG AKDAANETG TIVLSINPP AVTDLPFHL RNFYVELMA NISIELGAP PYLVTHEFN RVLQFEDEI KAAVVDEVQ NGQEIITTI DTLENQFPS IPWVHFLTN ITMNSNNIS SSSWIHFRA GRYLRNLQT LLHRTPKSI QANYIVWKI IEHFRNYLK ESVRSLYES FLIHLNPNL AFVNNRHLY CLQKSKFYF PSVTELAYV RTFLKPQSR ENIKDMALE IKYRLWKKI TANEWLDKN SKIFLLNKV AKIENIIGA SDHAFDQNF DQVVEMDGF DLDSDNIIE MATKLNAKR ADNYFNIIN QKSNEVAKE VLEKSPVLY INAFYAEDF NAMIVPASF LQGVIYDLD RPQYMNYGA LGSVIGHEY THSFTTFAE ENQTKTAES ADKYEGKIQ CLLEEYKEY GKHLSEFTT NAKSALEEN IADLIGPDL AYEAYQQWK WKNGPEPTL PGLNYTQNQ LFWIMSGAF KCYQPKRGF NIKFDQHHG VPSFAVTFS MRNSPFFAA DFNCPVGAN MNPEKKCRV L
- >TC009013 RA
atggtcaca gtttgtttc aaacaaaaa agcactttg atcaagtgc ttaattttc ctagtgtcc gtccttatc atgatttta gtcggattg gctatagtt ttggtgaag cgcaacgaa gatcaccac gtgtgcaag tcggatcac tgcatcaaa ctttcggcc gaactttta cgctccttg gaccagtcc agacacccc tgtgatgac ttctacgct ttcacatgc ggaggactt tcgcgaaat cgtaaggaa attcctccg gcttcaacg aaagaagtg ctgaatttt atggtggga gaaatggtc caaagcacc gactcgaag cctttcatt atggaaaaa tcgttttat ttagcttgt agtaacttg gctgggatt gagttggac gacgacgag tggttgctg gaccaattt tttagtctg ggagattgg ccggttctg cgtgggtct aactgggac ggttcgtac ttcgagtgg acggaaatc gtcaaaaag agtcgcaga ttaggcatg aaaatcgac tggtttgta gattttagt atctatgag gacgttaac gacacgaga gtcaatgtc ttgaagatt gatatacca gctctgacg cagctcaaa gaatcggaa gttttcccc agacagaaa ctgctcacc aaaattgcg gtcactttg ggcgccccg gaaaaactc gctcattat gaaacgggg agaaccatc gagtttgaa aaggaattg agaatgctt gcacatgta tacagaagc aggaaaaca catatgaac gcaaatact ctcccgaaa atcccaatc agtgaagtc cagaagctt tgcccccac atttcctgg ctggacttg atcaatgct gtgaccgga gtcaacatg caggaaacc gacgttgtg atttttgac gaaatggtc gactacatc aaagacttg aacaaattg atttacggg actccaaag aggattgtt gcggactac atgatgtgg aaagtggtc gaagaaaac tggcagttt ctcacgcaa ccggtccgc cagaagttc gaagaatac tacgaagaa cagcaccag atccaaccg gaacttgac cgtcaaaag ttttgcata aatgctagt agaaaacag tttccattg attgtcgag tccgagtat attcgtcgt catgtgacc ctccaaaaa agacaatta gtcaaggaa atgttaatt aatattcag cgcaactac cggcgatta ttacattcc gccacttgg atgaaccga aaacggaga gaattggcg ttggggaaa ttagacgag ctgaagttt ctaatcgga ggaccgcgg gaggttttc gacgaattt gggttcgct gaggatcta ggtctctat gagtttgtt ttcaaatca accaacatc attgatatg ctcaaccag agaaaactc cgattctac gaccactat ttcagttcg gttaacaag gccgttacg aaccggacg cagaaattc tacgagaaa gttatcaat ataaataga tttacggta ataaatgac gacacacta gtaatacca gccgcgtta ttaaaggac aacttttac gacccgaac cttccgaat aatttcaat tacgccaca ttggggaag tcgctagcc cacaaaata ttcgagcat attttcgag cgcaataat gtgaccatt attgacgag gagacgcag aattataac acgtggtgg atttcggac gggtttgat caagccgtc aaatgcctg attcacgaa gcctcggat ttccacaat ccggaatta aagaggcag aaaaaaatg cgtgaatta ttggcacat tttgtctca actgaggtc gcgtacgag tcgttcaag gaactactg acgtatcga caagattct ccacaatcc aagctgcaa tctttgcaa tacactccc gaacaatta ttctggata cacacgaac atgaatcca acaaactac gaagacgag cggagactt cacggttgt aagaaaaat aaattagct acctgtcta gccatcact attacgaca cttattttt gccctattt tttggcttt ttcggatat ttcggcttt ccagaaatt agcacttat tgtaatact ccggagtgt ctcaacgcc tcggcagaa ctctccgaa ataatcgac gactcgatc gatccctgt accgatttt tacagtttt gtgtgcgga aaactgatc aaaaatcaa aaaacccaa caaaattct ttaaaacaa gagtatgaa aaaatcgag caaacgctc gacgctctg attgtcggg ccggaaact ccggacgat tcccaagaa atccggatg caaaaacag tacttccaa gcgtgttta aacaccact aacattgat tcgaataag gaaatcact ttaatccga gaactaaac tcggtgggt ggatggccg ttcctccag aacagccga tggattgaa aaagagttc gactggatc aagtttatg atcagggct aggaaactg agattacct tttgagtgg tttctcaat gtgggactt tttttcgac aggggccaa tatcagttg aagctgtca caccccgac gattgtggg ctaaatatc aacgaggat tatttcaca atgcaacga aaaatcctg gagcaattg cgcaaacca ggcattgat gctatagtt gaattcacg caactggaa aactttgaa agaaaatta aattcgatt tgtgcgaac atgaccgat aacgatatt tccgtccgg agagcttcg aatctctgg ctggatggt attggaccc atctcgaat tttttaact tctgttact ggcctaaga gggtggaaa tcaaactcg tgggtggct tacaaccac aactatctc ttcgaattg aaccattta ttgaaactt ttttccaaa aaaactcaa gctttgttt attttgtgg aaaataatt cacttctac gccccttat ttatcaaaa ccgattcgt gaaaagtat ttcaattat ttggaaatc atgatgcaa agaaatatc agtcgggag gaattttgt cttgaagac actaaagaa aagtttaaa tacgtttct gaaattaaa tatctaaca gaaacggtt gatcctgag acacgagaa gaaggcaaa gggtttgtg gcgtcgctt gttaacgaa ataaccgac agaattgaa aatgataca attagaagg ttgcattcg gaaacttac agtgatttg gaggaagtt atagttgga ccagaagaa atttttgat gatacgttt atcgaagag tatttacaa ataggtcaa ttacacttc ttcactgat aacattatt ctaatgttg aatacgtgg tcccagaga atttttgaa aagaaaatg agtatgatt ggaaaaccc tttttatca ctagattca atatttgca aacgacggc gagtatagt atgacactg gatgaactc ttatctgta ccatctgtg acgttttac acttttttt aacaccgac gtgcaaaag tatttggaa tatggcagt ttgggaaca attgttggt acagaaatt gtccgatta gaacattac ctgaacggt gggaatcag aaacttgaa actaataaa aactgcatg attgaaaat atcaaaagt ttcaataga cgtttcaat gcatctatt gtttacgaa aaaaatttc ttagcctgg cttggtcaa gaattatcg taccaggcc taccagaaa tatacagat gaagaagga gcggaagca ccccttcca gatttgccc tacactccc aaccagctt ttctggata aaatcgagc ctgacgtgg tgccaggcc gccctcctt tcgcccgaa aattccttc aggacgttc caaatgttg cgaatcaaa gtcaccgaa tcggtgaaa cattcggag ttttttggg aaagatttc aactgtccc acagactcg cccatgaat ccagaaaac aagtgccaa ttattcgtt taccatgta agcgagaaa ccaaaaagt ggcaagaaa tggacgaga ctggaatta tttttggct ttagtggtc ataggcttg ggaatttat gttttttat ttgaggagt tacggtcaa aatgatgaa gaattgacg aacaagtgt ggagacatt tcttgctat aaaaccgta acagagctc caaaaattt atcgaacca tcggtcgat ccttgcgaa gattttcat cagttttcg tgtggcaag tttctagaa acagtccac acacaagaa ataacttct cgattagac caagaacaa acaaaatta gaaaacgag ttagaagcc atgattgtt gggcctatt ttaaataaa aattctcga cctgtgcaa atccaaaaa atgtttttc caatcttgc ctcaatttg gaggacatt gaagcggac aaaaatggg aaattcttg tctttggtc aaagagatt ggaggatgg cctcttgtt gaaggcaaa aaatggaac gaatcaaat tttgattgg aaagaagtt gccgtcaaa tgtagaaag ctgggattg ccgtttggg tggtttttg gagtttggc gtttacgaa gaagatcga aattatctt aaaattgcg tgcccaact catgtaaat cgcacaaac agtgtggac aaaattatt gtaaacttg atgggtaaa aaagcaaat attaaagac gtgatcgat tttgagcgg cgattactg catatctca cgtcctgaa acgcctgaa ataaatcta agaacaatt tcagaactg caaaaaata catccgcca gtggattgg ctagacttc ctcaataac ataacggaa gaaagttac gagtttacg gaagaaact gaaattgcc tctaatata gatgattac attttaaga ttgcacatg ctattaaca tcgactcca aagagcact caggctaac tacatcgtt tggaaactt ctagaacac tttgcgccc tttatacag agcggtgtc gaaaaaaaa ttagacagg ttcgagttt tgtaagcaa acgacaaag gaaatgttc cctgacttc tctcccatc gaatacatc cgaaaacac ccaacttcc aaagcccga aaatcagtt aaagaaatt gcagacttg gtaaaaaaa gaatttgtg gttcggatt caagatgcc acgtggtgg aatgaaacg gaaaagcag gtgtatttg gaactattt caaaactct ggctttctc ataggaaac cggattgaa aattttgag gatgatttt ctcgacgat cagtattta cacgtcaaa accatgatt ttagaagaa aattcggac gttattgaa atcttaact gcaaaatcg cgatcgtat tttaattcc atttttggc aaaataaac gaaagtagt tttatcaga gaagaaagt ttttccgaa tttatgaag gttgacata ttctttgac aaaaatttg aacaaaatg gttttgcca cctcctgca cttcatggg ttgcttttt gaccactcc cgcccccaa ttcatcaat tttgcgagt cttggcgat tctttggca aaagaatat gtgaataca cttcgtatt ctgactcac aactcgtcc gatgtggtg ttccaaacc agcgacaag tgcctggaa gccgactat ttaagtttg ggtggaaaa aacgaattt ccgtcggag ctaatttct gatattttg ggccgcgat atatcttac aaagcttat caaaaatgg atcgcgacc cacgtcgac gaatcccaa ctaccactc ttaccctac acaccaaac cagttgttt tggatcaag tcgagttta attaactgc caactaatc gattcgact ttaaaccat ctctcgatc agacattcg cgtcatttc ggcaaagat ttttcatgc gatagcagc ccgatgaat cctcgagaa aaattgacg gaaccccag ttttgcgtg actttgtcc tgcgtcgaa agctccaaa attatgaat aaaatggtc gatccgcgc aaaaatccg tgtgaggac tttcacgcg tacgtttgc ggaaagtct caatcaccc agagatgac agtcaaaga gtcctcaaa gaggtgctc tccgaaggc gacaaagtc aatgacaca gcatccttg aagctgcaa aagcaattc tatcactct tgcaacgat ttaaaggcc atagatatg gataacgat acgaccttt gcgcactta attgctaaa ttgggagga tggccggtc gtcagcggc aataattgg aacgaaagc gctttcgat tttgccgaa tttatggtg aatttgcgc gaattgggg ttccaatac gactggttt ttggacgtt tccgtctac tcgaatctg ggcgagaac atcattctt gagattaat gtgcccgac aatgtcaac aaaatcgaa gaaaaggac gaacaaaac tatgctgac ttgatggta gacgtagct atggctttc ggggcccac aaaagtgtg gcaaaatct caaatcccc gaagtagtt tatttggag cgtaaattg gctaatctt gtggatgaa attcaaacg gaaaacgaa gaattagcg aaacaaaaa tcgttccat ttgctgact gtagcggaa gtgcaacaa aaatggcaa aaattgaac tggttggag tttttaaat cacataact caaaagaag ctgtcagat acgcaatta gtggcgttt tatgtggag agttatatg ccaaaactt gacaagttg ttgaaacaa accacgaaa agaacacag gttaattac gttatctgg aaactaatc gagactttt tctccttat ttatccaaa ccaatccgc gacttgatg caaaactat tccataaaa gcaaacgaa actgaaacg tggtaccaa gaccgcagt accttctgt tttgaagag tccaagagt ttttttcag tacagtcta gaagccgaa ttcgttcgt cggtatgtt actgccacc aaaagaaga aacgtcgaa gagttaatt gagaggata ctaacccat ctcgaagaa cattttcgt gattccaaa tggttagat tttgaaaca cgaaaagcg gcgatttac aaaatacaa aacataagt tacattatt ggcggtccg gaggaaatg tattatgac aaacagttt gataaggat ttcgggatc ggagagctt gaattcaat tcgtcgaat ataatccac ataaacatg aaactgttt gaaaaagga ctcgacttg ctatttaat aagaaatac aaagagacg aattacctc aaatacacg ttttacgaa ctagtaccg tcgagggaa attcgattt atgcaacga tttaacttg atttgggta ccagctggg gccctccat ggttacttg tatgaggat accagaccc aattacctc aattatggt actttaggc accaccata gcccagcaa ttaatcgag gcaatgtat ttctcaatt ttgagaaat gtgtctgaa gttacgttc tccaatttc aaaaacatg actcattgt ttgcctcga tgtggatca gattccgag ctgatagat tgcaatgag agcctaata acaattatg tcgaattat ctcgctgtt gacatagca tacttggct tacaaaaga tgggagaag cagaatttc gtcgagaaa aacctgcac ggtttgcat tttagcggc gatcaatta ttctggttg gcttatgtg tgtatttta atcgcgttg ttttgtgtt ttcacgtta catgttaaa aacggtaat aggcacttt actcgacca gtggtgcaa aacgtgtcg tgtcctacg ccgagtcct ctccgagtc aaaaagtca actccctgt atgacccaa agctgcatc aaagccgcg tcgcaaata atcgacttc atcgaccct tctgtaaac ccgtgcgac aatttttac gattttgtg tgtggcaat tttctcaaa aaacctaaa gtctccaac gagataacg ccactgagc gccttagag gcagttact ttacaacaa ttcaattcg ttgatcacc gaacccatc aacgagaca ctgccaaaa tcatgggct ttgcaaagg aagtactac aacacgtgc atgaacact agtgccatc gaagaaaac aatagtgaa tctttctta gagatgttg aagtttctg ggagactgg ccactgctt catgggcgc ttctgggac gaaagaaaa ttcgattgg ttgaattcg atggtttta tgtcggaaa gttgggttt acatttgaa tattttctg aaaattgga gctaaagat gcggcaaat gaaaccgga acgattgtt ttatcaata aatccacca gctgtaacc gacttgccc tttcatttg agaaatttt tacgtcgag cttatggca aacatttcg atcgagttg ggggcgcct ccatatttg gtcactcat gagtttaac cgagtcttg caatttgaa gacgaaatc aaagcggcg gttgtggac gaggtgcaa aatggccaa gaaataatc acaacaatt gacacttta gaaaaccag tttccgtcg attccttgg gttcatttc ctcactaat atcacaatg aattcgaac aatatcagc tcaagctct tggatccat tttcgcgcc ggtcgctat ttgcgaaac ttgcaaact ttgctgcac cggacgccg aaatcaatc caagctaat tacatcgtg tggaaaatt atcgaacac tttcgcaac tacttgaaa gaaagcgtc cgttcgctt tacgaatca tttttaatc cacctcaac ccaaattta gctttcgta aacaatcgc catttgtac tgtttacag aaatcgaaa ttctatttt ccttcagtt acggaatta gcctacgtt cggacattc ctcaagccc cagtcgcgc gaaaacatc aaagacatg gcgctggag ataaaatac cgcctctgg aagaaaatc acagcaaat gagtggctc gacaaaaac tccaagatc ttcctcctg aacaaagtc gccaaaatc gaaaacata atcggagct tccgaccac gcgtttgac caaaacttc gaccaagtc gtcgagatg gacgggttt gacctcgat tcggataac atcatcgaa atggcaact aaactgaac gcgaaacga gccgacaac tacttcaac ataatcaac caaaagtcc aacgaggtg gccaaagaa gtgttggaa aaatcgccg gtcttgtac ataaacgcg ttttacgcc gaggatttt aacgcaatg atcgtaccg gcttcgttt ttgcaaggt gtaatctac gatcttgac cggccccaa tacatgaat tacggggct ttgggaagc gtcatcgga cacgagtac actcacagc tttacgacc tttgcggag gaaaaccaa accaagacc gcggaaagc gcggacaag tacgagggc aaaattcag tgcctcctg gaggagtac aaggagtac ggcaagcat ctgagcgag ttcacgacc aacgcgaaa tcggccctg gaagagaac atcgccgac ctaatcggc cccgattta gcctatgaa gcctaccag cagtggaaa tggaaaaac ggcccggag ccgacgctc cccggcctc aactacacc caaaaccag cttttctgg ataatgtcc ggagctttc aaatgctac cagcccaag cgcggtttt aacataaaa ttcgatcaa caccacggc gttccatcg ttcgccgtc actttcagt atgagaaac tctcctttt ttcgccgcc gacttcaat tgtcctgtc ggtgccaac atgaatccg gagaaaaaa tgtcgggtc ttgtga
- >TC009013 RA
ataattatt tttttcgtc ctacgcaca tgtgcatgg taaaatacc gaaaagtat cactcaata gcaacactt gtcgtatgt gtgaagaca ccaaacaca acaatggtc acagtttgt ttcaaacaa aaaagcact ttgatcaag tgcttaatt ttcctagtg tccgtcctt atcatgatt ttagtcgga ttggctata gttttggtg aagcgcaac gaagatcac cacgtgtgc aagtcggat cactgcatc aaactttcg gccgaactt ttacgctcc ttggaccag tccagacac ccctgtgat gacttctac gctttcaca tgcggagga ctttcgcga aatcgtaag gaaattcct ccggcttca acgaaagaa gtgctgaat tttatggtg ggagaaatg gtccaaagc accgactcg aagcctttc attatggaa aaatcgttt tatttagct tgtagtaac ttggctggg attgagttg gacgacgac gagtggttg ctggaccaa ttttttagt ctgggagat tggccggtt ctgcgtggg tctaactgg gacggttcg tacttcgag tggacggaa atcgtcaaa aagagtcgc agattaggc atgaaaatc gactggttt gtagatttt agtatctat gaggacgtt aacgacacg agagtcaat gtcttgaag attgatata ccagctctg acgcagctc aaagaatcg gaagttttc cccagacag aaactgctc accaaaatt gcggtcact ttgggcgcc ccggaaaaa ctcgctcat tatgaaacg gggagaacc atcgagttt gaaaaggaa ttgagaatg cttgcacat gtatacaga agcaggaaa acacatatg aacgcaaat actctcccg aaaatccca atcagtgaa gtccagaag ctttgcccc cacatttcc tggctggac ttgatcaat gctgtgacc ggagtcaac atgcaggaa accgacgtt gtgattttt gacgaaatg gtcgactac atcaaagac ttgaacaaa ttgatttac gggactcca aagaggatt gttgcggac tacatgatg tggaaagtg gtcgaagaa aactggcag tttctcacg caaccggtc cgccagaag ttcgaagaa tactacgaa gaacagcac cagatccaa ccggaactt gaccgtcaa aagttttgc ataaatgct agtagaaaa cagtttcca ttgattgtc gagtccgag tatattcgt cgtcatgtg accctccaa aaaagacaa ttagtcaag gaaatgtta attaatatt cagcgcaac taccggcga ttattacat tccgccact tggatgaac cgaaaacgg agagaattg gcgttgggg aaattagac gagctgaag tttctaatc ggaggaccg cgggaggtt ttcgacgaa tttgggttc gctgaggat ctaggtctc tatgagttt gttttcaaa tcaaccaac atcattgat atgctcaac cagagaaaa ctccgattc tacgaccac tatttcagt tcggttaac aaggccgtt acgaaccgg acgcagaaa ttctacgag aaagttatc aatataaat agatttacg gtaataaat gacgacaca ctagtaata ccagccgcg ttattaaag gacaacttt tacgacccg aaccttccg aataatttc aattacgcc acattgggg aagtcgcta gcccacaaa atattcgag catattttc gagcgcaat aatgtgacc attattgac gaggagacg cagaattat aacacgtgg tggatttcg gacgggttt gatcaagcc gtcaaatgc ctgattcac gaagcctcg gatttccac aatccggaa ttaaagagg cagaaaaaa atgcgtgaa ttattggca cattttgtc tcaactgag gtcgcgtac gagtcgttc aaggaacta ctgacgtat cgacaagat tctccacaa tccaagctg caatctttg caatacact cccgaacaa ttattctgg atacacacg aacatgaat ccaacaaac tacgaagac gagcggaga cttcacggt tgtaagaaa aataaatta gctacctgt ctagccatc actattacg acacttatt tttgcccta ttttttggc tttttcgga tatttcggc tttccagaa attagcact tattgtaat actccggag tgtctcaac gcctcggca gaactctcc gaaataatc gacgactcg atcgatccc tgtaccgat ttttacagt tttgtgtgc ggaaaactg atcaaaaat caaaaaacc caacaaaat tctttaaaa caagagtat gaaaaaatc gagcaaacg ctcgacgct ctgattgtc gggccggaa actccggac gattcccaa gaaatccgg atgcaaaaa cagtacttc caagcgtgt ttaaacacc actaacatt gattcgaat aaggaaatc actttaatc cgagaacta aactcggtg ggtggatgg ccgttcctc cagaacagc cgatggatt gaaaaagag ttcgactgg atcaagttt atgatcagg gctaggaaa ctgagatta ccttttgag tggtttctc aatgtggga ctttttttc gacaggggc caatatcag ttgaagctg tcacacccc gacgattgt gggctaaat atcaacgag gattatttc acaatgcaa cgaaaaatc ctggagcaa ttgcgcaaa ccaggcatt gatgctata gttgaattc acgcaactg gaaaacttt gaaagaaaa ttaaattcg atttgtgcg aacatgacc gataacgat atttccgtc cggagagct tcgaatctc tggctggat ggtattgga cccatctcg aatttttta acttctgtt actggccta agagggtgg aaatcaaac tcgtgggtg gcttacaac cacaactat ctcttcgaa ttgaaccat ttattgaaa cttttttcc aaaaaaact caagctttg tttattttg tggaaaata attcacttc tacgcccct tatttatca aaaccgatt cgtgaaaag tatttcaat tatttggaa atcatgatg caaagaaat atcagtcgg gaggaattt tgtcttgaa gacactaaa gaaaagttt aaatacgtt tctgaaatt aaatatcta acagaaacg gttgatcct gagacacga gaagaaggc aaagggttt gtggcgtcg cttgttaac gaaataacc gacagaatt gaaaatgat acaattaga aggttgcat tcggaaact tacagtgat ttggaggaa gttatagtt ggaccagaa gaaattttt gatgatacg tttatcgaa gagtattta caaataggt caattacac ttcttcact gataacatt attctaatg ttgaatacg tggtcccag agaattttt gaaaagaaa atgagtatg attggaaaa cccttttta tcactagat tcaatattt gcaaacgac ggcgagtat agtatgaca ctggatgaa ctcttatct gtaccatct gtgacgttt tacactttt tttaacacc gacgtgcaa aagtatttg gaatatggc agtttggga acaattgtt ggtacagaa attgtccga ttagaacat tacctgaac ggtgggaat cagaaactt gaaactaat aaaaactgc atgattgaa aatatcaaa agtttcaat agacgtttc aatgcatct attgtttac gaaaaaaat ttcttagcc tggcttggt caagaatta tcgtaccag gcctaccag aaatataca gatgaagaa ggagcggaa gcacccctt ccagatttg ccctacact cccaaccag cttttctgg ataaaatcg agcctgacg tggtgccag gccgccctc ctttcgccc gaaaattcc ttcaggacg ttccaaatg ttgcgaatc aaagtcacc gaatcggtg aaacattcg gagtttttt gggaaagat ttcaactgt cccacagac tcgcccatg aatccagaa aacaagtgc caattattc gtttaccat gtaagcgag aaaccaaaa agtggcaag aaatggacg agactggaa ttatttttg gctttagtg gtcataggc ttgggaatt tatgttttt tatttgagg agttacggt caaaatgat gaagaattg acgaacaag tgtggagac atttcttgc tataaaacc gtaacagag ctccaaaaa tttatcgaa ccatcggtc gatccttgc gaagatttt catcagttt tcgtgtggc aagtttcta gaaacagtc cacacacaa gaaataact tctcgatta gaccaagaa caaacaaaa ttagaaaac gagttagaa gccatgatt gttgggcct attttaaat aaaaattct cgacctgtg caaatccaa aaaatgttt ttccaatct tgcctcaat ttggaggac attgaagcg gacaaaaat gggaaattc ttgtctttg gtcaaagag attggagga tggcctctt gttgaaggc aaaaaatgg aacgaatca aattttgat tggaaagaa gttgccgtc aaatgtaga aagctggga ttgccgttt gggtggttt ttggagttt ggcgtttac gaagaagat cgaaattat cttaaaatt gcgtgccca actcatgta aatcgcaca aacagtgtg gacaaaatt attgtaaac ttgatgggt aaaaaagca aatattaaa gacgtgatc gattttgag cggcgatta ctgcatatc tcacgtcct gaaacgcct gaaataaat ctaagaaca atttcagaa ctgcaaaaa atacatccg ccagtggat tggctagac ttcctcaat aacataacg gaagaaagt tacgagttt acggaagaa actgaaatt gcctctaat atagatgat tacatttta agattgcac atgctatta acatcgact ccaaagagc actcaggct aactacatc gtttggaaa cttctagaa cactttgcg ccctttata cagagcggt gtcgaaaaa aaattagac aggttcgag ttttgtaag caaacgaca aaggaaatg ttccctgac ttctctccc atcgaatac atccgaaaa cacccaact tccaaagcc cgaaaatca gttaaagaa attgcagac ttggtaaaa aaagaattt gtggttcgg attcaagat gccacgtgg tggaatgaa acggaaaag caggtgtat ttggaacta tttcaaaac tctggcttt ctcatagga aaccggatt gaaaatttt gaggatgat tttctcgac gatcagtat ttacacgtc aaaaccatg attttagaa gaaaattcg gacgttatt gaaatctta actgcaaaa tcgcgatcg tattttaat tccattttt ggcaaaata aacgaaagt agttttatc agagaagaa agtttttcc gaatttatg aaggttgac atattcttt gacaaaaat ttgaacaaa atggttttg ccacctcct gcacttcat gggttgctt tttgaccac tcccgcccc caattcatc aattttgcg agtcttggc gattctttg gcaaaagaa tatgtgaat acacttcgt attctgact cacaactcg tccgatgtg gtgttccaa accagcgac aagtgcctg gaagccgac tatttaagt ttgggtgga aaaaacgaa tttccgtcg gagctaatt tctgatatt ttgggccgc gatatatct tacaaagct tatcaaaaa tggatcgcg acccacgtc gacgaatcc caactacca ctcttaccc tacacacca aaccagttg ttttggatc aagtcgagt ttaattaac tgccaacta atcgattcg actttaaac catctctcg atcagacat tcgcgtcat ttcggcaaa gatttttca tgcgatagc agcccgatg aatcctcga gaaaaattg acggaaccc cagttttgc gtgactttg tcctgcgtc gaaagctcc aaaattatg aataaaatg gtcgatccg cgcaaaaat ccgtgtgag gactttcac gcgtacgtt tgcggaaag tctcaatca cccagagat gacagtcaa agagtcctc aaagaggtg ctctccgaa ggcgacaaa gtcaatgac acagcatcc ttgaagctg caaaagcaa ttctatcac tcttgcaac gatttaaag gccatagat atggataac gatacgacc tttgcgcac ttaattgct aaattggga ggatggccg gtcgtcagc ggcaataat tggaacgaa agcgctttc gattttgcc gaatttatg gtgaatttg cgcgaattg gggttccaa tacgactgg tttttggac gtttccgtc tactcgaat ctgggcgag aacatcatt cttgagatt aatgtgccc gacaatgtc aacaaaatc gaagaaaag gacgaacaa aactatgct gacttgatg gtagacgta gctatggct ttcggggcc cacaaaagt gtggcaaaa tctcaaatc cccgaagta gtttatttg gagcgtaaa ttggctaat cttgtggat gaaattcaa acggaaaac gaagaatta gcgaaacaa aaatcgttc catttgctg actgtagcg gaagtgcaa caaaaatgg caaaaattg aactggttg gagttttta aatcacata actcaaaag aagctgtca gatacgcaa ttagtggcg ttttatgtg gagagttat atgccaaaa cttgacaag ttgttgaaa caaaccacg aaaagaaca caggttaat tacgttatc tggaaacta atcgagact ttttctcct tatttatcc aaaccaatc cgcgacttg atgcaaaac tattccata aaagcaaac gaaactgaa acgtggtac caagaccgc agtaccttc tgttttgaa gagtccaag agttttttt cagtacagt ctagaagcc gaattcgtt cgtcggtat gttactgcc accaaaaga agaaacgtc gaagagtta attgagagg atactaacc catctcgaa gaacatttt cgtgattcc aaatggtta gattttgaa acacgaaaa gcggcgatt tacaaaata caaaacata agttacatt attggcggt ccggaggaa atgtattat gacaaacag tttgataag gatttcggg atcggagag cttgaattc aattcgtcg aatataatc cacataaac atgaaactg tttgaaaaa ggactcgac ttgctattt aataagaaa tacaaagag acgaattac ctcaaatac acgttttac gaactagta ccgtcgagg gaaattcga tttatgcaa cgatttaac ttgatttgg gtaccagct ggggccctc catggttac ttgtatgag gataccaga cccaattac ctcaattat ggtacttta ggcaccacc atagcccag caattaatc gaggcaatg tatttctca attttgaga aatgtgtct gaagttacg ttctccaat ttcaaaaac atgactcat tgtttgcct cgatgtgga tcagattcc gagctgata gattgcaat gagagccta ataacaatt atgtcgaat tatctcgct gttgacata gcatacttg gcttacaaa agatgggag aagcagaat ttcgtcgag aaaaacctg cacggtttg cattttagc ggcgatcaa ttattctgg ttggcttat gtgtgtatt ttaatcgcg ttgttttgt gttttcacg ttacatgtt aaaaacggt aataggcac tttactcga ccagtggtg caaaacgtg tcgtgtcct acgccgagt cctctccga gtcaaaaag tcaactccc tgtatgacc caaagctgc atcaaagcc gcgtcgcaa ataatcgac ttcatcgac ccttctgta aacccgtgc gacaatttt tacgatttt gtgtgtggc aattttctc aaaaaacct aaagtctcc aacgagata acgccactg agcgcctta gaggcagtt actttacaa caattcaat tcgttgatc accgaaccc atcaacgag acactgcca aaatcatgg gctttgcaa aggaagtac tacaacacg tgcatgaac actagtgcc atcgaagaa aacaatagt gaatctttc ttagagatg ttgaagttt ctgggagac tggccactg cttcatggg cgcttctgg gacgaaaga aaattcgat tggttgaat tcgatggtt ttatgtcgg aaagttggg tttacattt gaatatttt ctgaaaatt ggagctaaa gatgcggca aatgaaacc ggaacgatt gttttatca ataaatcca ccagctgta accgacttg ccctttcat ttgagaaat ttttacgtc gagcttatg gcaaacatt tcgatcgag ttgggggcg cctccatat ttggtcact catgagttt aaccgagtc ttgcaattt gaagacgaa atcaaagcg gcggttgtg gacgaggtg caaaatggc caagaaata atcacaaca attgacact ttagaaaac cagtttccg tcgattcct tgggttcat ttcctcact aatatcaca atgaattcg aacaatatc agctcaagc tcttggatc cattttcgc gccggtcgc tatttgcga aacttgcaa actttgctg caccggacg ccgaaatca atccaagct aattacatc gtgtggaaa attatcgaa cactttcgc aactacttg aaagaaagc gtccgttcg ctttacgaa tcattttta atccacctc aacccaaat ttagctttc gtaaacaat cgccatttg tactgttta cagaaatcg aaattctat tttccttca gttacggaa ttagcctac gttcggaca ttcctcaag ccccagtcg cgcgaaaac atcaaagac atggcgctg gagataaaa taccgcctc tggaagaaa atcacagca aatgagtgg ctcgacaaa aactccaag atcttcctc ctgaacaaa gtcgccaaa atcgaaaac ataatcgga gcttccgac cacgcgttt gaccaaaac ttcgaccaa gtcgtcgag atggacggg tttgacctc gattcggat aacatcatc gaaatggca actaaactg aacgcgaaa cgagccgac aactacttc aacataatc aaccaaaag tccaacgag gtggccaaa gaagtgttg gaaaaatcg ccggtcttg tacataaac gcgttttac gccgaggat tttaacgca atgatcgta ccggcttcg tttttgcaa ggtgtaatc tacgatctt gaccggccc caatacatg aattacggg gctttggga agcgtcatc ggacacgag tacactcac agctttacg acctttgcg gaggaaaac caaaccaag accgcggaa agcgcggac aagtacgag ggcaaaatt cagtgcctc ctggaggag tacaaggag tacggcaag catctgagc gagttcacg accaacgcg aaatcggcc ctggaagag aacatcgcc gacctaatc ggccccgat ttagcctat gaagcctac cagcagtgg aaatggaaa aacggcccg gagccgacg ctccccggc ctcaactac acccaaaac cagcttttc tggataatg tccggagct ttcaaatgc taccagccc aagcgcggt tttaacata aaattcgat caacaccac ggcgttcca tcgttcgcc gtcactttc agtatgaga aactctcct tttttcgcc gccgacttc aattgtcct gtcggtgcc aacatgaat ccggagaaa aaatgtcgg gtcttgtga atagattgt tttcgtgtg tgttcacac atcctcatt attcaacgg gcaacggtt aattataat gat
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